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The Cerrado domain has numerous plant species that naturally produce essential oils, that are include in one of several classes of secondary metabolites. The objective of this study was to characterize the chemical composition and to evaluate the antioxidant and antifungal activities of the essential oil from Cochlospermum regium flowers. The flowers from C. regium were collected in an area of Cerrado, in Rio Verde/GO, Brazil. To extract the essential oil, 100 g of flowers were used, and then they were subjected to hydrodistillation in Clevenger-type apparatus, and the essential oil yield, determined in percentage. Then, the essential oil was analyzed for composition by gas chromatography with coupled mass spectrometry GCMS, and determined as to the antioxidant potential in reducing DPPH and, as antifungal by agar diffusion in the isolate from Sclerotinia sclerotiorum. The essential oil was analyzed by gas chromatograph associated with mass spectrometry CG-MS and its antioxidant potential was evaluated by the free radical sequestration technique DPPH. The average yield of essential oil was 0.10%. 32 compounds were identified, in especially majority caryophyllene E 9.76%, γ-muurolene 16.69% and bicyclogermacrene 39.82%. The antioxidant activity showed 100% reduction in the DPPH radical, for concentrations between 50 to 2 mg mL-1, and reductions of 85.77 to 13.18% (IC50) for concentrations 1 to 0.031 mg mL-1 of the essential oil. It is concluded that the essential oil from C. regium showed low yield, however, it contains many hydrocarbon and oxygenated sesquiterpenes compounds, presenting itself as an exceptional natural antioxidant and antifungal agent in the fight against Sclerotinia sclerotiorum. Thus, the essential oil of the flowers from C. regium presents itself as strong candidate for new research that can be applied in the areas from health, agricultural and foods. [ABSTRACT FROM AUTHOR]

The quality and content of essential oils extracted from medicinal and culinary plants can be affected by harvesting time conditions. This study focused on the antioxidant activity and chemical composition of Origanum dictamnus essential oils under different harvesting time conditions. Samples from the same area and different harvesting times were treated by hydrodistillation. Gas Chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS) were used to determine the different compounds found in essential oils. In all the essential oil samples, the phenolic monoterpenoid Carvacrol was prevalent. Through different seasons, we could observe a relatively high yield ratio. However, the lowest and highest yield values were found in plants collected during winter and summer. These essential oils showed high level radical scavenging properties level, as exhibited in the DPPH assay. Essential oils from sample C (collected on 10/02/18) showed the highest antioxidant activity. [ABSTRACT FROM AUTHOR]

The essential oil obtained by microwave-assisted hydro-distillation (MAHD) and hydrodistillation (HD) techniques from the peel of grapefruit (Citrus paradisi. L) from Algeria was analyzed by gas chromatography-flame ionization detector (GC-FID) and gas chromatography ⁄ mass spectrometry (GC⁄ MS). Twenty-one constituents were identified in essential oils obtained by HD and twentyeight constituents in essential oils by MAHD, representing respectively 99.11% and 99.74% of the total oil. The main constituents were limonene (85.54%-87.51%) for MAHD and HD, β-myrcene (2.99%-3.24%), nootkatone (1.78%-1.80%). The antimicrobial activity of the essential oils was evaluated by disc diffusion method. The results showed interested inhibition growth against the tested bacteria (Escherichia coli, Pseudomonas aeruginos, and Staphylococcus aureus)and the yeast (Candida albicans),with an inhibition zone ranging from 4 to 20 mm.Both essentials oils showed no effect against Aspergilusniger, Verticillium sp and Thielaviopsis sp. The insecticidal activity was investigated against adults ceratitis capitata. Both essential oils showed a toxic effect against this insect (LD50:9.12μl EO/ml of acetone; LD90: 13.18 μl EO/ml of acetone). [ABSTRACT FROM AUTHOR]

Syzygium cumini (L.) Skeels (Jambolan) is a widespread summer fruit plant, well-known for various therapeutic uses such as anti-diabetic, antimicrobial, antioxidant properties. The fruit helps to convert starch into energy which helps in regulating blood sugar levels and possesses a low glycemic index. The present study is aimed to estimate the chemical composition of the essential oil of leaves of Syzygium cumini through GC/MS. The essential oil was extracted by hydrodistillation using a Clevenger apparatus which was further analyzed through GC/MS. The GC/MS analysis identified twenty-five components with a total area percentage of 95.66 %. The major identified components were τ-cadinol (21.44 %) and tau-muurolol (12.01 %). The compounds of intermediate concentration were globulol (7.98 %), caryophyllene (6.69 %), δ-cadinene (6.56%) and α-pinene (6.32 %). While β-eudesmol (4.42 %), β-pinene (4.32 %), γ-cadinene (3.87 %), camphene (2.98 %), α-terpinenol (2.72 %), camphor (2.23 %), humulene 6,7-epoxide (2.17 %), cubeban-11-ol (1.98 %), α-muurolene (1.81 %), epicubenol (1.43 %), α-copaene (1.26 %), viridiflorene (1.02 %), β-guanine (0.65 %), β-bourbonene (0.23 %), terpinen-4-ol (0.13 %), endo-borneol (0.12 %), levoverbenone (0.12 %) and isobornyl acetate (0.12 %) were the compounds present in minor concentrations. Among the identified compounds there were monoterpene hydrocarbons (13.62 %), bicyclic-monoterpenoid (0.36 %), terpenoid ketones (2.23 %), monocyclic monoterpenoid alkene alcohol (2.85 %), non-oxygenated sesquiterpenes (25.17 %) and oxygenated sesquiterpenes (51.43 %). The present investigation also discusses the applications of various components found in S. cumini leaf essential oil and a comparative view on the essential oil composition of the leaves of the plant was reported earlier. [ABSTRACT FROM AUTHOR]

The chemical compositions and functional properties of Zanthoxylum chalybeum (Knob wood) leave essential oils varied as growing locations. In this study, the variation in chemical composition and antimicrobial activities of the hydro distilled essential oils of the plant fresh leaves collected from three different growing places in Eastern Ethiopia (Awale, Dire Dawa Administration; Awaday, Haramaya subdistrict, and Lange, Kersa Subdistrict, East Hararghe Zone of Oromia Region), were investigated. The chemical composition of the respected essential oils, as determined by the GC-MS analysis, 13, 12 and 17 compounds representing 99.98%, 100.00% and 100.00% of the total leaves essential oil from the plant growing in Awale, Awaday, and Lange, respectively, were identified. The essential oils of Awale, Awaday and Lange were mostly β-phellanderene (23.96%, 45.31%, 23.45% respectively), whereas highest amount of other components in the essential oils of Lange: terpine-4-ol (25.02%) and sabinene (11.73%); Awaday: α-phellanderene (12.85%) and Awale: the sole β-myrcene (29.33%) was identified. In vitro antimicrobial activity in 20 μL dose of each essential oil evaluated by the disc diffusion method indicated strong antibacterial activities against the test bacteria (S.thyphi, Str. agalactia, S.aureus and E.coli ) and slight antifungal activities against the test fungi (A.niger, F.oxysporium). The essential oils' major constituents, the monoterpenoids, such as phellandrenes (α and β), terpinen-4-ol and sabinene could be responsible for the significant antimicrobial activities in this study. The plant essential oils' chemical composition and the antimicrobial activities significantly differ with growing locations. The findings confirm the plant's medicinal properties in traditional uses. The results open the many possibilities for further research on biocidal and antimicrobial activities of the essential oil and its individual components of the species growing in different regions of Ethiopia. [ABSTRACT FROM AUTHOR]

This study investigated the potential use of microwave energy as an effective technique for the extraction of essential oil (EO) from some plant species belonging to Lamiaceae family growing in Egypt, involving Origanum majorana L., Mentha pipereta L., Mentha longifolia L., Origanum syriacum L., Lavandula angustifolia L., Rosmarinus officinalis L., and Thymus vulgaris L., in comparison with conventional extraction using hydrodistillation (HD) approach, in terms of extraction time, oil yields, and chemical composition. The results showed that the use of microwave in a relevant technique, namely microwave‐assisted hydrodistillation (MAHD) required an extraction of 60 minutes, while HD required 180 minutes. In general, EO yield being extracted by MAHD was higher than those extracted by HD. Gas chromatography in combination with mass spectrometry (GC/MS) analysis indicated noticeable quantitative changes in EOs composition extracted by MAHD and HD extraction methods. Except for O majorana L., GC/MS analysis of extracted oils using MAHD showed higher amounts of oxygenated constituents compared to HD extraction. The results of this study introduced MAHD as rapid, economical and environmental friendly extraction method. A comparative analysis was stated here between the EOs of all plant species under study on chemometric tools such as principal components analysis (PCA) and agglomerative hierarchical clustering (AHC). In general, the PCA results showed that there is significant variation between species in the quality and quantity of EO composition. However, no significant difference is observed based on the extraction method used (HD and MAHD). [ABSTRACT FROM AUTHOR]

Chemical composition of essential oils hydrodistilled from aerial parts of Achillea fragrantissima (Forssk.) Sch. Bip. and Achillea santolina L. growing in Jordan was analysed using GC and GC/MS. The analysis identified 30 compounds in the oil isolated from A. santolina, and 23 and 22 constituents in the oils hydrodistilled from A. fragrantissima collected from north and south Jordan, respectively. Eucalyptol was the chief oil constituent of A. santolina (25.2 %) oil, while, β-sesquiphellandrene (28.6 %) and α-thujone (33.8 %) were the principal constituents of A. fragrantissima oils collected from north and south Jordan, respectively. Achillea santolina oil had a higher overall percentage of monoterpenoid content compared to the oils of A. fragrantissima of north and south Jordan, with oxygenated monoterpenes comprising the largest portion of this monoterpenoid content. On the other hand, essential oils obtained from A. fragrantissima were richer in sesquiterpenoids, with sesquiterpene hydrocarbons constituting the highest percentage of this content. [ABSTRACT FROM AUTHOR]

The yield and composition of Japanese peppermint (Mentha arvensis L.) essential oil obtained by solvent-free microwave extraction were determined and compared with those obtained by traditional hydrodistillation. The essential oil was obtained in 0.88-0.95 % yield by treatment at 100-500 W for 20-90 min, and these yields are similar to those obtained by hydrodistillation. Thus, solvent-free microwave extraction is a suitable distillation method for the extraction of essential oils from Japanese peppermint. [ABSTRACT FROM AUTHOR]

This paper reports the chemical constituents of essential oils from various parts of Decaspermum parviflorum (Lam.) J. Scott. (Myrtaceae) from Vietnam. The essential oils were obtained by hydrodistillation and analyzed by gas chromatography with flame ionization detection (GC-FID) and gas chromatography coupled to mass spectrometry (GC/MS). The main constituents of the oils were β-elemene (1.68-4.14%), caryophyllene (14.53-43.98%), humulene (3.99-10.74%), eudesma-4(14),(11)-diene (4.83-17.46%), a-selinene (3.65-13.60%), cadina- 1(10),4-diene (1.0-3.17%), and seline-3,7(11)-diene (1.13-3.20%). Caryophyllene oxide (2.38-3.63%), ylangene (1.22-3.20%), guaia-3,9- diene(1.46-4.50%), eudesma-4(14)-en-11-ol (2.07-2.24%), neointermedeol (1.39-3.25%), aromadendrene oxide-(2) (1.05-1.65%), and naphthalene,1,2,3,4,4a,5,6,7-octahydro-4a,8-dimethyl-2-(1-methylethenyl)- (0.73-2.47%), which were identified only in the flower and fruit. Ocimene (11.87%) and -elemene (37.02%) were identified only in the flower. Copaene (8.27%) was identified only in the leaf. This is the first report of the chemical constituents of essential oils from the leaf, fruit, and flower of D. parviflorum, and these constituents differ from those reported for other Decaspermum oils. [ABSTRACT FROM AUTHOR]

Background and Aim: The rhizomes of Curcuma caesia have traditional applications for pain healing and are an imperiled species of India. A study on investigation of in vitro anti-oxidant and acetylcholinesterase (AChE) inhibitory efficacy of essential oil from the rhizome part was carried out through extraction by hydrodistillation. Materials and Methods: The phyto components present in this volatile oil were identified by gas chromatography-mass spectrometric analysis and based on Spearman's correlation, the cooccurrence network of the 21 compounds were analyzed. In vitro anti-oxidant, anti-AChE activities were assessed with different concentrations of essential oil by spectrophotometric analysis, following the standard protocols. Results: The studies revealed that major compounds identified were Androsta-1,4-dien-3-one,17-(acetyloxy)-, (17.beta.)-Santanol acetate (16.11%), Eucalyptol (12.98%), Cycloprop[e]indene-1a,2(1H)-dicarboxaldehyde,3a,4,5,6,6a,6b-hexahydro-5,5,6b-trimethyl, (1a. alpha., 3a. beta., 6a. beta., 6b. alpha) (8.96%), methyl 7,12-octadecadienoate (6.75%), and (+)-2-Bornanone (6.60%). The major interactions of terpenoidal compounds with other compounds give a picture that terpenoids have a very significant role as anti-oxidants and cholinesterase inhibitors. The essential oil showed the total phenolic content of 26.72 ± 0.38 mg/g of gallic acid equivalent and total flavonoid content of 18.92 ± 0.27 mg/g of quercetin equivalent (QE). 2, 2-diphenyl-1-picrylhydrazyl and 2, 2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid radical scavenging 50% inhibitory concentration (IC50) values of the oil were 186.33 ± 0.46 μg/mL and 109.41 ± 0.43 μg/mL. The hydrogen peroxide and nitric oxide radical scavenging IC50swere 103.45 ± 0.58 μg/mL and 190.55 ± 0.75 μg/mL, respectively. The AChE inhibitory IC50value was 156.33 ± 0.44 μg/mL. Conclusion: The study demonstrated on essential oil extracted from rhizomes of C. caesia indigenous to Arunachal Pradesh, India, has moderate anti-oxidant property and would be a valuable non-toxic source for managing neurodegenerative diseases. [ABSTRACT FROM AUTHOR]

We determined the chemical composition and explored the hypothesis that the essential oil of the fruits of Pimenta dioica inhibits the mycelial development of fungi Fusarium oxysporum f. sp. lycopersici, F. oxysporum f. sp. passiflorae, F. subglutinans f. sp. ananas, F. oxysporum f. sp. vasinfectum e F. oxysporum f. sp. Cubense. To do this, we extracted the oil by hydrodistillation, identified its components by gas chromatography coupled to mass spectrometry (GC-MS) and determined the fungal activity against five special forms of Fusarium species. The results showed that the oil had 76.88% of eugenol and inhibited the mycelial development of fungi up to 97.78% in an average of 7.2 days. Therefore, oil is a potential natural fungicide. [ABSTRACT FROM AUTHOR]

In this present study, we determined the chemical composition and we tested the toxicity and the molluscicidal activity of the essential oil extracted from the barks of Citrus sinensis (L.) Osbeck. For this, the essential oil was extracted quantitatively by hydrodistillation. Then, quantifications of its components were performed by gas chromatography coupled to mass spectrometry (CG-MS) and the toxicity and molluscicidal activity were tested, respectively, against Artemia salina and snails Biomphalaria glabrata. The results showed that the oil contains 81.50% of D-limonene (main constituent) and 0.06% of citronellal (minor component) and it has lethal activity (LC50) of 100.08 mg.L-1 and a toxicity, with LC50 of 321.84 mg.L -1 at a 95% confidence level. Therefore, the oil is active against the snail Biomphalaria glabrata and non-toxic to other living bein. [ABSTRACT FROM AUTHOR]

Amphiphilic compounds have the ability to alter the plant morphology which may improve the conventional process for isolating essential oil. In the present study, various hydrotropes, surfactants, ionic liquids, and sodium salt have been assessed in extracting essential oil from Cymbopogon winterianus grass using hydrodistillation. The highest yield (4.18%) of oil was attained using 5% NaCl solution without affecting the quality of oil. This approach has reduced the extraction time, which further reduced the utility consumption, power consumption, and carbon footprints. Moreover, incorporation of sonication has provided 4.08% yield in 15 min, thereby further improving the process. [ABSTRACT FROM AUTHOR]

Acmella oleracea is an important food and medicinal plant in the Amazon; it is used to treat toothaches, hepatitis, malaria, fever is also described as an anticancer plant. This work reports for the first time the comparison of chemical profiles, antioxidant, and cytotoxic capacities of inflorescence (INF) and leaf (LEA) A. oleracea essential oils (EOs) obtained by hydrodistillation (HD) and steam distillation (SD). The essential oils were obtained by hydrodistillation and steam distillation and analyzed by GC and GC-MS. The antioxidant potential was evaluated by the co-oxidation method of the β-carotene/linoleic acid, and the cytotoxic activity against healthy human embryonic kidney (HEK-293), gastric ascites (AGP-01), melanoma (SK-MEL-19), lung carcinoma (A549) malignant cell lines by the MTT method. The essential oils obtained from INF by HD and SD showed the highest yields (HD = 0.68; SD = 0.5%). The monoterpene hydrocarbons (0–49.97%) and sesquiterpene hydrocarbons (25.79–50.63%) were predominant in the analyzed oils. The main constituents (>10%) identified in the EO samples were caryophyllene oxide (0.3–28.11%), E -caryophyllene (6.71–27.31%), myrcene (0–25.03%), germacrene D (0.06–19.56%), guaiol (0–14.23%), 1-pentadecene (0–13.88%), and β-pinene (0–10.04%). The chemometric analysis displayed the formation of three distinct groups of chemical compositions as follows: group I, LEA EOs (HD and SD); group II, INF EO (HD); and group III, INF EO (SD). The INF (14.3%) and LEA (9.0%) EOs showed antioxidant action with no significant difference (p < 0.05). The INF EO (HD) showed higher cytotoxicity against the malignant lineages of gastric ascites (IC50 5.31 μg/mL), melanoma (IC50 6.43 μg/mL), while the LEA EO (SD) was more cytotoxic against the lung carcinoma cell line (13.39 μg/mL), and higher toxicity was also observed against the healthy embryonic kidney cell line (IC50 5.14 μg/mL). Acmella oleracea is chemically influenced by the volatile extraction methods, and this influences on the antioxidant or cytotoxic capacity of the species. • Acmela oleracea is a food with ethnopharmacological interest in the Amazon region. • It is used in different countries to treat toothache, hepatitis, malaria, and fever. • The inflorescence essential oil (EO) is rich in myrcene and E -caryophyllene. • The leaf EO contains abundantly caryophyllene oxide. • The oils were anti-lipoperoxidative and were cytotoxic towards human cancer cells. [ABSTRACT FROM AUTHOR]

Helichrysum decorum DC, Helichrysum lepidissimum S. Moore, and Helichrysum umbraculigerum are three species traditionally used in the South African medicine. The present work deals with the investigation of the spontaneous emission and the essential oils obtained from these plants cultivated in open field under uniform conditions. Fractions of the volatile organic compounds of the three species were rich in monoterpene hydrocarbons, representing more than 70% of the total composition. Pinene isomers were the most representative compounds: β-pinene in H. decorum (53.0%), and α-pinene in H. lepidissimum (67.9%) and H. umbraculigerum (54.8%). These latter two species evidenced an important amount of sesquiterpene hydrocarbons (SH) especially represented by γ-curcumene (H. lepidissimum) and α- and β-selinene (H. umbraculigerum). On the contrary, in the EOs, sesquiterpenes compounds prevailed, representing more than 64% of the identified fraction to reach more than 82 and 87% in H. umbraculigerum and H. lepidissimum, respectively. Although the chemical classes and their relative abundances were comparable among the three species, the individual compounds of EOs showed large differences. In fact, caryophyllene oxide (26.7%) and γ-curcumene (17.4%) were the main constituents in H. decorum, and H. lepidissimum respectively, while neo-intermedeol (11.2%) and viridiflorol (10.6%) characterized H. umbraculigerum.

The essential oil (EO) of the desert cotton (Aerva javanica (Burm. f.) Juss. ex Schul.) was extracted by hydrodistillation, from A. javanica flowers growing in the sandy soils of the United Arab Emirates (UAE) wild desert. The influence of seasonal variation on flowers’ EO yield was studied. The flowers’ EO yield obtained from spring samples (0.011%) was significantly the highest followed by early summer (0.009%), winter (0.007%), and autumn samples (0.006%), respectively. The flowers’ EO antioxidant analysis were tested by DPPH, FRAP and ABTS assays (in vitro). Results proved that A. javanica flowers’ EO, isolated during the four seasons, is a good source of natural bioactive antioxidants. Based on the three tested assays, the highest antioxidant activity was recorded in the spring. Testing of the chemical composition of the flowers’ EO was conducted for the season with the highest yield and the best antioxidant performance, recorded in spring, by a combination of gas chromatograph (GC) and gas chromatograph-mass spectrometer (GC-MS). This led to the identification of 29 volatile components, in which the flowers’ oil was characterized by angustione as a major compound. Photos by scanning electron microscope (SEM) showed prominent availability of star-shaped trichomes in the epidermis of the flowers.

Four taxa of the Lamiaceae family growing in Apulia (Clinopodium suaveolens, Satureja montana subsp. montana, Thymbra capitata, and Salvia fruticosa subsp. thomasii) that had not been previously studied for their potential use in the food sector, were analyzed for their essential oils (EOs) composition and antioxidant and antimicrobial properties against some microorganisms, isolated from bread and bakery products, including molds (Aspergillus niger, Penicillium roqueforti) and spore-forming bacteria (Bacillus amyloliquefaciens and Bacillus subtilis). Two different sites were considered for each plant species, and the strongest antimicrobial EOs, which were active against all of the microorganisms tested, were those from one S. montana subsp. montana sample (Sm2) and both T. capitata EOs (Tc1 and Tc2) with Minimal Inhibitory Concentration (MIC) values ranging between 0.093% and 0.375% (v/v) against molds, while higher values were registered for bacteria (0.75–1%). In particular, the biological activity of EOs from T. capitata and S. montana subsp. montana was maybe due to the high amount of thymol and carvacrol, which were also responsible for the highest antioxidant activity. S. fruticosa subsp. thomasii EOs had different chemical profiles but showed only a slight antibacterial effect and no antifungal activity. C. suaveolens showed no significant changes between EOs with an interesting antifungal activity (MIC 0.093%÷0.187% v/v), which may be due to the presence of pulegone. These plant species can be considered as promising sources of bioactive compounds to be exploited as biopreservatives in bread and bakery products mainly considering the low concentration needed to inhibit microorganism’s growth.

Frangipani oil is one of the most expensive essential oils. Conventional extraction methods has many disadvantages, so the microwave hydrodistillation method is used as an alternative to optimize the quality and quantity of frangipani oil. The purpose of this study was to investigate the effect of microwave power on the yield of frangipani oil. The research stage was carried out by inserting frangipani flower that has been cut into the flask and mixed with different aquadest solvent volumes of 300, 400, 500, and 600 ml. The flask put into the microwave with different powers of 300, 450, and 600 W for three hours. The resulting vapor is collected and separated in a separating funnel. The process is carried out at atmospheric pressure and the operating temperature is 100 °C. Finally, the yield percentage determined from resulting product. The results showed that microwave power had a significant effect on the yield percentage of frangipani oil. The yield percentage increased with increasing the microwave power. The highest yield percentage was generated at 600 Watt, 25 gram flower mass, and 600 ml volume, which was 1,612%.

The glucosinolate rich hot and cold hexane extracts of Brassica juncea had a negative effect on the development of Spodoptera litura larvae when they were fed on different concentrations of the extracts. Both larval growth index and pupal growth index declined with treatment. However the hot extract with 3-butenyl isothiocyanate as the predominant compound had a more deleterious effect as at 3125ppm the larvae were unable to complete their development. The nutritional indices too were more adversely affected with hot extract as compared to cold extract. The RGR, RCR, ECI and ECD declined significantly with increase in concentration of the hot hexane extract. The activity of antioxidant enzymes, SOD and catalase decreased while that of phosphatases, GSTs, phenol oxidases increased during the initial treatment duration but decreased on prolonged treatment of the larvae with LC50 concentration of hot extract. A similar trend was observed for glutathione and lipid peroxides but a decrease in ascorbate content was observed as compared to control. The findings reveal a toxic effect of 3-butenylisothiocyanate rich hot hexane extract of B. juncea on S. litura larvae.

Abstract Background The volatile constituents from the aerial parts of Mentha piperita L. (peppermint) which were collected from cultivate growing plants in Kermanshah (Garreban; at the east of Kermanshah City) of Iran were extracted by hydrodistillation and headspace/solid-phase micro-extraction (HS/SPME) methods and were analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Findings A total of 39 and 41 compounds were identified in the essential oil by hydrodistillation and HS/SPME methods, respectively. The main components in the hydrodistillation method were menthol (45.34%), menthone (16.04%), menthofuran (8.91%), cis-carane (8.70%), 1,8-cineole (4.46%), neo-menthol (4.24%), and limonene (2.22%). The main components in the HS/SPME method were menthol (29.38%), menthone (16.88%), cis-carane (14.39%), menthofuran (11.38%), 1,8-cineole (9.45%), trans-caryophyllene (2.76%), neo-menthol (2.37%), β-Pinene (2.26%), α-Pinene (1.55%), germacrene-D (1.41%), trans-sabinene hydrate (1.28%), and neoisomenthyl acetate (1.02%). Conclusions The medicinal herb Mentha piperita L. (peppermint) would be changed during hydrodistillation method by chemical changes on the main essential oil of this herb before heat effects in presence of water vapor matrix (WVM).

This paper presents kinetics modeling of essential oil hydrodistillation from juniper berries (Juniperus communis L.) by using a non-linear regression methodology. The proposed model has the polynomial-logarithmic form. The initial equation of the proposed non-linear model is q = q∞•(a•(logt)2 + b•logt + c) and by substituting a1=q∞•a, b1 = q∞•b and c1 = q∞•c, the final equation is obtained as q = a1•(logt)2 + b1•logt + c1. In this equation q is the quantity of the obtained oil at time t, while a1, b1 and c1 are parameters to be determined for each sample. From the final equation it can be seen that the key parameter q∞, which presents the maximal oil quantity obtained after infinite time, is already included in parameters a1, b1 and c1. In this way, experimental determination of this parameter is avoided. Using the proposed model with parameters obtained by regression, the values of oil hydrodistillation in time are calculated for each sample and compared to the experimental values. In addition, two kinetic models previously proposed in literature were applied to the same experimental results. The developed model provided better agreements with the experimental values than the two, generally accepted kinetic models of this process. The average values of error measures (RSS, RSE, AIC and MRPD) obtained for our model (0.005; 0.017; –84.33; 1.65) were generally lower than the corresponding values of the other two models (0.025; 0.041; –53.20; 3.89) and (0.0035; 0.015; –86.83; 1.59). Also, parameter estimation for the proposed model was significantly simpler (maximum 2 iterations per sample) using the non-linear regression than that for the existing models (maximum 9 iterations per sample). [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. TR-35026]

This paper deals with fractionation of the essential oil from juniper (Juniperus communis L.) berries by hydrodistillation (HD) and simultaneous hydrodistillation and rectification (SHDR) at atmospheric pressure. A rectification column was filled with the NORMAG packing A type and had 36 theoretical stages. In the present study, higher essential oil yield was obtained by HD (1.34 mass %) than by SHDR (1.17 mass %). However, mass fraction distributions of monoterpene hydrocarbons (MHs), oxygenated monoterpenes (OMs) and sesquiterpene hydrocarbons (SHs), observed for the two separation methods, differed from each other. In the SHDR process increased contents of high volatile components (predominantly MHs) were obtained in the initial fractions as well as increased contents of low volatile components (dominantly SHs) in the last fractions. Also, this method increased the separation degree of OMs. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 033007 and Grant no. 173021]

Fresh leaves of N. glauca were collected from the northern region of Tunisia. The leaves were submitted to water distillation for 4 h, using a Clevenger-type apparatus. The obtained essential oils were dried over anhydrous sodium sulphate and after filtration, stored at 4 °C until use. The chemical composition of the isolated essential oil was analyzed by gas chromatography–mass spectrometry (GC–MS). Eighteen compounds were identified: eugenol, the major compound in the essential oil, was present at a high level (58.49%), followed by nonadecane, eugenyl acetate and tridecane, 3-methyl at 6.38; 5.57 and 5.19%, respectively. The percentage of compounds dodecane, 2, 6, 11 trimethyl, tetradecane, docosane, tricosane and 1, 2-benzene dicarboxilic, dibutyl ester varied between 1 and 2%; whereas the other compounds (including limonene, and saturated hydrocarbons) remained at low percentages, not exceeding 1%. This study could be very useful for the characterization, pharmaceutical and therapeutic applications of the essential oil from N. glauca.

Background: Essential oils have been used for medical purposes because of their medicinal properties and are sourced from natural products. Aim: The comparative study of essential oils from Clausena anisata leaves obtained through two methods of extraction was undertaken. Setting: C. anisata leaves were collected from Akinsola village in Ido Local Government Area of Oyo State, Nigeria. Methods: Essential oil was extracted using the methods of solvent-free microwave extraction (SFME) and hydrodistillation (HD) and then analysed using gas chromatography coupled with mass spectrometer (GC-MS). Results: The result shows that the essential oils obtained from the leaves of C. anisata through SFME and HD are pale yellow and colourless, respectively. A higher oil yield of 5.2 mL/100 g was obtained through SFME compared to 3.7 mL/100 g obtained through HD. There was an abundance of monoterpenes in the oil obtained using HD, while sesquiterpenes were the dominant compounds in the oil extracted using SFME. Other compounds present were α-cubebene, α-copaene and α-pinene with 1091.63, 1091.64 and 979.35, respectively. Conclusion: The compounds obtained which are very important in the cosmeceutical and pharmaceutical industries, is dependent on the method of essential oil extraction.

The aim of this study was to provide a chemical profile and determine the antioxidant and antimicrobial activity of the essential oil (EO) and lipid extracts of Thymus serpyllum L. herbal dust obtained via conventional (hydrodistillation (HD) and Soxhlet extraction (SOX)) and novel extraction techniques (supercritical fluid extraction (SFE)). In addition, a comparative analysis of the chemical profiles of the obtained EO and extracts was carried out, as well as the determination of antioxidant, antibacterial and antifungal activity of the lipid extracts. According to the aforementioned antioxidant and antimicrobial activities and the monoterpene yield and selectivity, SFE provided significant advantages compared to the traditional techniques. In addition, SFE extracts could be considered to have great potential in terms of their utilization in the pharmaceutical and cosmetic industries, as well as appropriate replacements for synthetic additives in the food industry.

This study explores the impact of plasma treatment on Lavandin Grosso flowers and its influence on the extraction of essential oils (EOs) via hydrodistillation. Short plasma treatment times enhance the yield of EO extraction from 3.19% in untreated samples to 3.44%, corresponding to 1 min of plasma treatment, while longer treatment times (10 min) show diminishing returns to 3.07% of yield extraction. Chemical characterization (GC/MS and ATR-FTIR) indicates that plasma treatments do not significantly alter the chemical composition of the extracted EOs, preserving their aromatic qualities. Investigations into plasma-surface interactions reveal changes at the nanometer level, with XPS confirming alterations in the surface chemistry of Lavandin Grosso flowers by reducing surface carbon and increasing oxygen content, ultimately resulting in an increased presence of hydrophilic groups. The presence of hydrophilic groups enhances the interaction between the surface membrane of the glandular trichomes on Lavandin Grosso flowers and water vapor, consequently increasing the extraction of EOs. Furthermore, microscopic SEM examinations demonstrate that plasma treatments do not affect the morphology of glandular trichomes, emphasizing that surface modifications primarily occur at the nanoscale. This study underscores the potential of plasma technology as a tool to enhance EO yields from botanical sources while maintaining their chemical integrity.

Essential oils are of great interest due to their potent pharmaceutical and biological activities. In this study, essential oils extracted from Origanum compactum and Thymus zygis originating from the Middle Atlas of Morocco were investigated. Their chemical compositions were analyzed using gas chromatography and mass spectrometry, while the assessment of the trapping power of the radical (DPPH: 1,1-diphenyl-2-picrylhydrazyl) and the reducing antioxidant potential of ferric ions (FRAP: Ferric Reducing Antioxidant Power) were performed in order to evaluate the antioxidant activity. Their antibacterial potency was tested against six bacterial strains through the disk diffusion method. The chromatography analyses of the extracted essential oils highlighted the presence of two main components, namely carvacrol at 75.70% in O. compactum and thymol at 40.67% in T. zygis . The antioxidant activity tests showed that both essential oils demonstrated a significant antioxidant activity comparable to the positive control (e.g., ascorbic acid). The antibacterial activity results showed a strong antimicrobial effect for both essential oils, compared to synthetic antibiotics. This study affirms the presence of bioactive components with interesting antioxidant and antibacterial activities in the essential oils extracted from Origanum compactum and Thymus zygis , which could find several applications in the food and pharmaceutical industries through the substitution of synthetic antioxidants and antibiotics.

Introduction: Larch oleoresin has been described regarding several biological activities and medicinal applications, such as wound healing and treatment of ulcers, but little is known about its chemical composition., Material and Methods: Eight oleoresins from Larix decidua Mill. obtained from four companies and one adulterated control were therefore investigated to determine their content of essential oils and to verify possible differences in their composition in relation to the harvest and manufacturing processes. Essential oils (EOs) were isolated by distillation and the yield was analysed., Results and Discussion: The yield of EO varied among all samples. The yield of the pure larch samples covered a range of 7.8% to 15.5%. A higher yield (19.0%) was observed for adulterated control, which contained oleoresins from different Pinaceae trees. Age of samples had no impact on yield. However, there was a significant statistical variation (p<0.05) in the yields of the mid-summer oleoresins (>10%) compared to early or late summer (<10%), emphasising the importance of the time of collection. Samples were subsequently analysed by GC-MS. EO samples confirmed the presence of various chemical classes, such as monoterpenes, sesquiterpenes, and diterpenes. α-pinene was the compound with the highest concentrations (>50%), followed by β-pinene (>6%), D-limonene (>2.5%), α-terpineol (>0.9%), β-myrcene (>0.2%), and 3-carene (>0.05%). Samples were grouped using multivariate data analysis (MVDA) with respect to the chemical variation between the oleoresins' EOs. The resulting four clusters were named low (low yield obtained for the samples), mixed (mixed oleoresin from different Pinaceae species, adulteration control), old (old oleoresin kept in the institute), and normal (other oleoresins) samples, each presenting distinct chemical biomarkers. There were considerable differences between site and time of collection. Essential oil yield did not always meet requirements as defined by the German Homeopathic Pharmacopoeia. In addition, adulterated or aged samples could be identified as compared to pure and fresh larch oleoresins., Conclusion: We conclude that larch oleoresin used for pharmaceutical applications has to be carefully analysed and standardised to guarantee reproducible product quality., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Batista, Melo, Holandino, Maier, Huwyler, Baumgartner and Boylan.)

Consumers are becoming more mindful of their well-being. Increasing awareness of the many beneficial properties of peppermint essential oil (EO) has significantly increased product sales in recent years. Hydrodistillation (HD), a proven conventional method, and a possible alternative in the form of microwave-assisted hydrodistillation (MWHD) have been used to isolate peppermint EO. Standard Soxhlet and alternatively supercritical fluid (SFE), microwave-assisted, and ultrasound-assisted extraction separated the lipid extracts. The distillations employed various power settings, and the EO yield varied from 0.15 to 0.80%. The estimated environmental impact in terms of electricity consumption and CO2 emissions suggested that MWHD is an energy efficient way to reduce CO2 emissions. Different extraction methods and solvent properties affected the lipid extract yield, which ranged from 2.55 to 5.36%. According to the corresponding values of statistical parameters, empiric mathematical models were successfully applied to model the kinetics of MWHD and SFE processes.

The objective of this work was to determine the chemical composition, the antioxidant and antibacterial activities of the essential oils (EOs) extracted by hydrodistillation from different organs of the D. viscosa: flowers, leaves and aerial parts. The main compounds identified by GC/MS are oxygenated sesquiterpenes. Among these compounds, (E)-nerolidol (40.7%) is the most abundant constituent of flowers' essential oil while caryophyllene oxide (9.9%), isolongifolan-7-α-ol (10.3%) and α -eudesmol (9.1%) are the major constituents of the leaves' essential oil. The presence of these compounds in the aerial parts' essential oil is solely due to those of the flowers and leaves that constitute these aerial parts. The volatile extracts showed antioxidant effects with IC50 values ranging between 9.25 and 9.75 mg.mL−1. On the other hand, EOs showed antibacterial effects on both Gram-positive and Gram-negative bacteria. The highest activity was obtained with flowers' essential oil against Enterococcus faecalis and Escherichia coli. [ABSTRACT FROM AUTHOR]

The chemical compositions were investigated for pine essential oils obtained through a solvent-free microwave assistance extraction system (ME) and a conventional hydrodistillation system (HD). The essential oils of P. pinea, P. nigra, P. brutia, and P. sylvestris sawdust were analyzed by gas chromatography and mass spectroscopy (GCMS). The main components of the pine essential oils were D-limonene (52.8% for ME and 76.6% for HD), β-caryophyllene (12.4% for ME), β-myrcene (2.89% for ME and 1.48% for HD), sesquiterpene hydrocarbons (25% for ME and 9.79% for HD), and total sesquiterpene (25.25% for ME and 9.79% for HD) for P. pinea; D-limonene (28.1% for ME and 79.2% for HD) for P. nigra; α-pinene (76.6% for ME and 77.3% for HD), diterpene hydrocarbons (94.17% for ME and 95.62% for HD), and total diterpenes (94.94% for ME and 96.3% for HD) for P. brutia; β-pinene (36.7% for ME and 42.4% for HD), terpineol, (13.8% for ME and 6.06% for HD) diterpene alcohol (26% for ME and 12.57% for HD), and total oxygenated terpenes (26% for ME and 12.57% for HD)for P. sylvestris. Moreover, the ME was able to produce more diterpene alcohols and sesquiterpene hydrocarbons while the HD showed higher potential for the diterpene hydrocarbons. According to the heat-map correlation, P. pinea showed high similarity with P. nigra, while P. sylvestris was related to P. brutia. [ABSTRACT FROM AUTHOR]

Subcritical water extraction has been considered due to advantages such as high speed, high efficiency and reduced amount of organic solvents used in the traditional way as well as due to its compatibility with the environment. In this study, the essences extraction from fennel seeds was performed by using subcritical water extraction and was compared with hydrodistillation method. Trans-anethole as the main combination has been carried out as a main goal of next experiments. Separating and diagnosis of the extracted trans-anethole were performed by GC-FID and GC / MS. The effect of temperature parameters, Average of particle size and flow intensity was studied on the extraction rate in subcritical water method. Taguchi experimental design was used to facilitate the process of testing and comparing the effective parameters. The ratio of the area of trans-anethole to the obtained internal standard from gas chromatograpHy was calculated as evaluated criteria of parameters and extraction methods of essence from fennel seeds. The results of Analysis of variance showed that the temperature and, Average of particle size have a significant effect on the amount of trans-anethole extraction. The highest ratio of trans-anethole to internal standard was obtained at 175 °C, an average value of 0.398 mm, and a flow rate of 3 ml /min. The results of this study showed that the extraction by subcritical water in compare with hydro distillation method is a fast-high-efficiency method for extraction of trans-anethole as a valuable oxygen-containing compound. [ABSTRACT FROM AUTHOR]

• Essential oils (EOs) were obtained for the first time from aromatic plant byproducts. • Solvent-free microwave extraction was applied to obtain EOs from byproducts. • Thirteen volatile compounds were quantified in EOs of S. montana by GC–MS. • Carvacrol was the most abundant component (825–950 μg/mg) of S. montana EOs. • S. montana EOs exhibited antimicrobial activity against poultry pathogenic bacteria. Satureja montana is a worldwide consumed aromatic plant whose essential oils (EOs) are used as spice and preservative by food industry. Only the leaves are marketed, generating a large amount of stems as byproducts. It is possible that the EOs present in these byproducts represent a source of compounds with antimicrobial activity, as observed for the leaves. In this work, dried S. montana byproducts were used to extract EOs. Although differences were observed for the harvest year (0.42 ± 0.11 mg/g in 2016 and 0.14 ± 0.07 mg/g in 2017), monoterpenoids were the major components, accounting for 84.4–97.6%, being carvacrol the most abundant component (825–950 μg/mg). Sesquiterpenoids accounted for 0.3 to 0.5%. Similar EOs yield and composition were obtained using solvent-free microwave extraction (16 min using 100 g of S. montana stems, previously, soaked in water and drained) or hydrodistillation (120 min using 1000 mL of water/ 100 g of dried S. montana stems). The in vitro minimal inhibitory concentrations (MICs) of S. montana EOs against Escherichia coli ATCC 25922, Salmonella enterica sv Anatum SF2, and Staphylococcus aureus ATCC 6538, which belong to pathogenic species with economic impact in poultry industry, were 225 μg/mL, 250 μg/mL, and 150 μg/mL, respectively. As these MICs are similar to the ones reported for carvacrol against the same or related strains, it can be concluded that carvacrol is the active compound in S. montana byproducts. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the effect of low-pressure dielectric barrier discharge (DBD) plasma on microwave-assisted hydrodistillation of lemon peel oil extraction. Microwave pre-treated lemon peel powder was exposed to plasma treatment (1.0, 1.5, 2.0, and 2.5 kV) for 10 min. The treated lemon peel powders were subjected to hydrodistillation to extract the essential oil and the extraction yields were calculated. The extracted oil was analyzed for chemical composition with gas chromatography-mass spectrometry (GC-MS). Effect of plasma on the surface morphology of the lemon peel was observed in a scanning electron microscope (SEM) which revealed the formation of fissures and cracks owing to the higher extraction yield. Plasma treatment at 2.5 kV was observed higher extraction yield than conventional hydrodistillation (149.34 % rise) and the chemical composition of plasma treated sample essential oil remains significantly unchanged. Thus, DBD plasma could be a promising technique to enhance the lemon peel essential oil extraction. [ABSTRACT FROM AUTHOR]

In the present study, peel oils were extracted through hydrodistillation and cold pressing from three Citrus species (Valencia orange, Ponkan and Eureka lemon) to investigate their volatile constituents and antioxidant activities. A total of 47 volatile components were identified by GC–MS, and then grouped by principal component analysis. The extraction methods were found to have an obvious effect on the proportion of terpenes and oxygenated compounds in the six Citrus oils, especially for Eureka lemon oils. The major fractions in the Citrus oils were found to be monoterpenes (78.65–96.57%), with limonene occupying a dominant percentage (51.22–86.65%). Furthermore, γ-terpinene and terpinolene displayed strong DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging abilities and efficient inhibition of lipid peroxidation, while oxygenated compounds of α-terpineol and terpinen-4-ol showed poor DPPH radical-scavenging abilities. Therefore, hydrodistillated Eureka lemon oil with high levels of α-terpineol (9.11%) and terpinen-4-ol (4.69%) presented low radical scavenging capability. Citral displayed a high pro-oxidant ability against thiobarbituric acid reactive species formation, which might lead to the decreased ability of the Eureka lemon oils in inhibition of lipid peroxidation, since citral was significantly high in Eureka lemon oils. This study facilitated the understanding of volatile constituents and antioxidant activities in different Citrus peel oils. [ABSTRACT FROM AUTHOR]

The present work aimed to evaluate the effect of drying air temperature on the essential oil content of lemongrass leaves, as well as the adjustment of different mathematical models to the experimental values of water content ratio. The treatments used prior to oil extraction were: four drying temperatures (35, 45, 55 and 65 °C) and one control (fresh plant). For the extraction of the essential oil the hydrodistillation method was employed, using the apparatus called Clevenger. It was concluded that the drying time of lemongrass leaves decreases as the air temperature increases. The temperature of 55 °C for the lemongrass drying air provides higher oil yield compared to the other drying treatments (35 45 and 65 °C). The Midilli model best represents the drying kinetics of lemongrass leaves. The diffusion coefficient will increase as the drying air temperature rises from 1.2746 x 10-11 to 5.9217 x 1011 m² s-1. The activation energy for liquid diffusion during drying of lemongrass leaves is 63.75 kJ mol-1. [ABSTRACT FROM AUTHOR]

The rising discourse over concerns on climate change due to non environmental compatibility of traditional industrial processes have fuelled the development of digitized, high performing, ecofriendly technologies which are in tandem with environment. In the context of extraction of essential oil, drawbacks of classical hydrodistillation such as thermal degradation, high energy consumption, time consuming, low yield have made way for more greener approach. The novelty in the proposed work lies in the development of a rapid, solvent free, gravity assisted microwave based extraction model. The model ensures complete bio-refinery of the residual biomass for extraction of other non-volatile principles with special emphasis to nutraceuticals. The developed working protocol is based on a novel approach of delivering a high power microwave surge concept. An initial high power microwave surge at 60% power level was applied for 2 min, followed by a surge at 40% power level for 2 min and finally followed by sustained microwave firing at 20% power level for 6 min. The above conditions resulted in extraction of 10 mL (dehydrated) of oil from 60 g fresh mentha leaves. The yield of oil was 5 times higher than conventional hydrodistillation method for 6 h. The results of extraction yield were found to be in tandem with the oil quality (volatile principles and biological activity). The biomass obtained after extraction of oil retained the phenolics, flavonoids and other nutraceutical principles thus making it suitable for re-extraction. Image 104176 •The research advocates "Turn GREEN, be sustainable". •The proposed method is a solvent free technique producing more yield in lesser time. •The proposed method supports refinery of biomass and ensure safe and high quality products. [ABSTRACT FROM AUTHOR]

The objective of the present study was the optimization of the parameters affecting the hydrodistillation of Ruta chalepensis L. essential oil using response surface design type Box- Behnken. After an appropriate choice of three parameters, 15 experiments were performed leading to a mathematical second-degree model relating the response function (yield of essential oil) to parameters and allowing a good control of the extraction process. The realization of the experiments and data analysis was carried out by response surface methodology (RSM). A deduced second-order polynomial expression was used to determine the optimal conditions necessary to obtain a better essential oil yield. These optimized operating conditions were: a granulometry of 2 mm, a condensation-water flow rate of 3.4 mL/min and an extraction time of 204 min. Analysis of variance (ANOVA) indicates that the generated second-order polynomial model was highly significant with R2=0.9589 and P<0.006. The gas chromatography-mass spectrometry analysis of essential oil extracted from the Ruta chalepensis L. aerial parts revealed the presence of 2-undecanone, 2- nonanone and 2-decanone as major components. [ABSTRACT FROM AUTHOR]

The aim of this research was to investigate the effect of different extraction methods and chemical composition of the essential oil and solid-phase microextraction (SPME) from Rhododendrum caucasium Pall. The volatiles of R. caucasicum have been isolated by hydro distillation (HD) and SPME. The compositions of the volatiles were characterized by GC-FID/MS. A total of twenty-five and thirty-one compounds were identified constituting over 89.25%, and 90.33% of volatiles obtained with HD and SPME, respectively. The main volatile constituents of R. caucasicum were found to be calarene (46.13% (HD) and 54.91% (SPME)) and sandaracopimaradiene (25.93% (HD) and 8.16% (SPME)). Furthermore, the obtained essential oil (EO) and solvent extracts (n-hexane and methanol) of R. caucasicum were tested against the following nine bacteria: Escherichia coli, Yersinia pseudotuberculosis, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus, Mycobacterium smegmatis, Candida albicans, and Saccharomyces cerevisiae. The EO showed moderate antimicrobial activities with the inhibition zone from 6 to18 mm against E. faecalis, S. aureus, B. cereus and M. smegmatis, respectively. Methanol extract gave better antimicrobial activity against the P. aeruginosa, E. faecalis, S. aureus, and B. cereus with the almost 15 mm inhibition zones. [ABSTRACT FROM AUTHOR]

Basil essential oil (BEO) contains a wide range of chemical compounds whose content may vary depending on chemotypes, environmental conditions, agronomic techniques and particularly the origin of the plant. In our present study, essential oils (EOs) were isolated by hydrodistillation method from dry herbs of three basil cultivars and analyzed by GC-FID and GC-MS. Two of the tested cultivars belong to sweet basil group (B1 and B2) while the third one was large leafed 'Genovese' basil (B3). EO content in the dry herb was 0.65%, 0.41% and 0.62% respectively. The main classes of compounds of B1EO and B3EO were sesquiterpene hydrocarbons (38.39% and 37.95%), oxygenated monoterpenes (25.44% and 28.04%) and phenylpropanoids (17.43% and 15.71%). The main constituents of both EOs were monoterpene alcohol linalool (13.68% and 15.38%), phenoyl derivate eugenol (10.83% and 8.97%) and sesquiterpene hydrocarbon α-bergamotene (8.12% and 9.25%). In both EOs, epi-bicyclosesquiphellandrene was detected in considerable amount (7.03% and 8.07%). The most abundant compound classes in B2EO were oxygenated monoterpenes (52.07%), sesquiterpene hydrocarbons (24.27%) and phenylpropanoids (10.95%). Linalool was the dominant compound (40.97%), followed by epi-bicyclosesquiphellandrene (8.70%) and methyl chavicol (7.92%). The results showed complex chemical composition of BEOs and pointed out the presence of biologically active compounds of importance for different branches of the pharmaceutical, chemical and food industry. Although there are differences in the chemical composition of the BEOs, the obtained results show that all of the tested cultivars are rich in compounds which are responsible for biological activities. [ABSTRACT FROM AUTHOR]

Fresh leaves of N. glauca were collected from the northern region of Tunisia. The leaves were submitted to water distillation for 4 h, using a Clevenger-type apparatus. The obtained essential oils were dried over anhydrous sodium sulphate and after filtration, stored at 4 °C until use. The chemical composition of the isolated essential oil was analyzed by gas chromatography--mass spectrometry (GC--MS). Eighteen compounds were identified: eugenol, the major compound in the essential oil, was present at a high level (58.49%), followed by nonadecane, eugenyl acetate and tridecane, 3-methyl at 6.38; 5.57 and 5.19%, respectively. The percentage of compounds dodecane, 2, 6, 11 trimethyl, tetradecane, docosane, tricosane and 1, 2-benzene dicarboxilic, dibutyl ester varied between 1 and 2%; whereas the other compounds (including limonene, and saturated hydrocarbons) remained at low percentages, not exceeding 1%. This study could be very useful for the characterization, pharmaceutical and therapeutic applications of the essential oil from N. glauca. [ABSTRACT FROM AUTHOR]

The essential oil of Persian black cumin was extracted using static supercritical CO2 technique at different temperatures, extraction times, and number of extractions. The composition of extracted oil was measured by GC-MS analysis and 25 components were identified. It was found that the oil recovery was reduced by increasing the temperature. Maximum recovery was achieved at two-step extraction that was about 49%. Both dissolution rate and solubility limited the oil recovery at the tested extraction conditions. The oxygenate components have less dissolution rate than the terpenes. The application of experimental data for design of an efficient dynamic extraction system was discussed. [ABSTRACT FROM AUTHOR]

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Background: Essential oils are chemical products produced by odoriferous glands from a variety of plants. These essential oil have many health benefits i.e. antiseptic, anti-inflammatory and antimicrobial activities. So due to these medicinal properties present study was designed to analyze essential oil of Thymus vulgaris and Mentha arvensis for their chemical composition and biological activities. Materials and methods: Essential oil from these plants were extracted by hydrodistillation method, and analyzed by GC-MS. To test the microbial activity of these oil disk diffusion method and micro wells method were used. For free radical scavenging DPPH assay was used. However total phenolic content was measured by colorimetric method. Results: The GC-MS analysis of T. vulgaris oil showed the presence of 47 chemical compounds among which thymol, terpinene, p-cymene and carvacrol were major. However essential oil of M. arvensis showed the presence of 28 constituents, among which the Menthone, Menthol, Isomenthone, Eucalyptol, neo-Menthol, cis-Piperitone oxide, Linalool, Thymol, Limonene, and α-Phellandrene were major. Essential oil from both these plant tested for antimicrobial activity showed that the T. vulgaris oil was effective against seven bacterial strains and the essential oil of M. arvensis was effective against six bacterial strain. The antioxidant activity of both samples by DPPH assay which showed positive result. Conclusion: As both species showed the presence of active components, positive microbial activities, and antioxidant activity so, research should be carried on for further biological activities of these oil for betterment of living beings. [ABSTRACT FROM AUTHOR]

Highlights • Mace contains useful essential oils. • Mace extraction using MAHD is more efficient than HD. • More oxygenate compositions are found in essential oils extraction using MAHD. • Diffusion-controlled model can describe the mechanism of mace extraction well. • Microwave power plays an significant role on essential oils diffusivity. Abstract Mace essential oil extractions were carried out using hydrodistillation (HD) and microwave assisted hydrodistillation (MAHD). The powers used were varied from 300, 600, and 800 W. The amounts of oil from dew resulted from condensation were measured every 1 min. The condensation was done using 12 °C cold water. From the experimental results, MAHD is seen to be superior to HD. The extraction using MAHD at 800 W stopped after 20 min (8.63% yield), while the one using HD stopped after 73 min (7.03% yield). The power used affected the yields of essential oils obtained. From the various powers used, in 10 min, the yields obtained were 2.68, 4.56, 5.41%, in 15 min 4.12, 6.20, 6.83%, and in 20 min 5.13, 7.39, 6.83% at 300, 600, and 800 W, respectively. It can also be seen that time has a significant effect on the essential oil yields obtained. The main components of the essential oil obtained from HD and MAHD were beta pinene, alpha pinene, myristicin, 4-terpineol, and gamma terpinene. The mechanism of mace essential oil mass transfer using HD and MAHD methods was controlled by intra-particle diffusion following the Fick's law. The diffusion coefficient (De) of HD was 4.98 × 10−14 m2/s and the diffusion coefficients of MAHD were 9.17 × 10−14, 1.39 × 10−13, and 1.65 × 10−13 m2/s at 300, 600, and 800 W, respectively. The empirical correlation of diffusion coefficient and MAHD power can be approximated by De = 3.02 × 10−15 × P 0.5985. [ABSTRACT FROM AUTHOR]

The bioactive ingredients of many essential oils are complex and difficult to reproduce synthetically. In the present study, the influence of extraction method on relative extraction of bioactive compounds of essential oil of Platycladus orientalis (L.) Franco (Thuja orientalis L. is a synonym of it) leaves gathered from semi-arid climate was studied. A higher yield of essential oil was obtained by hydrodistillation (0.1%) as compared to steam distillation (0.07%) under newly optimized conditions. Initial analysis of these oils by TLC showed the presence of different polarity groups ranging from non-polar terpene hydrocarbons to polar terpenoid alcohols. GC-MS analysis revealed that major component of hydrodistilled essential oil of Platycladus orientalis (L.) Franco leaves was α-pinene (17.83%) and steam distilled essential oil contained α-cedrol (12.44%). The results obtained in the present study emphasize that suitable extraction technique should be used to obtain particular component of interest. [ABSTRACT FROM AUTHOR]