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Imidazole compounds are important constituents of atmospheric brown carbon. The imidazole components of aqueous secondary organic aerosol (aqSOA) that are generated from the evaporation of droplets containing pyruvaldehyde and inorganic ammonium are on-line characterized by an aerosol laser time-of-flight mass spectrometer (ALTOFMS) and off-line detected by optical spectrometry in this study. The results demonstrated that the laser desorption/ionization mass spectra of aqSOA particles that were detected by ALTOFMS contained the characteristic mass peaks of imidazoles at m/z = 28 (CH2N+), m/z = 41 (C2H3N+) and m/z = 67 (C3H4N2+). Meanwhile, the extraction solution of the aqSOA particles that were measured by off-line techniques showed that the characteristic absorption peaks at 217 nm and 282 nm appeared in the UV-Vis spectrum, and the stretching vibration peaks of C-N bond and C=N bond emerged in the infrared spectrum. Based on these spectral information, 4-methyl-imidazole and 4-methyl-imidazole-2-carboxaldehyde are identified as the main products of the reaction between pyruvaldehyde and ammonium ions. The water evaporation accelerates the formation of imidazoles inside the droplets, possibly owing to the highly concentrated environment. Anions, such as F−, CO32−, NO3−, SO42− and Cl− in the aqueous phase promote the reaction of pyruvaldehyde and ammonium ions to produce imidazole products, resulting in the averaged mass absorption coefficient () in the range of 200–600 nm of aqSOA increases, and the order of promotion is: F− > CO32− > SO42− ≈ NO3− ≈ Cl−. These results will help to analyze the constituents and optics of imidazoles and provide a useful basis for evaluating the formation process and radiative forcing of aqSOA particles.

Retinal vessel segmentation is extremely important for risk prediction and treatment of many major diseases. Therefore, accurate segmentation of blood vessel features from retinal images can help assist physicians in diagnosis and treatment. Convolutional neural networks are good at extracting local feature information, but the convolutional block receptive field is limited. Transformer, on the other hand, performs well in modeling long-distance dependencies. Therefore, in this paper, a new network model MTPA_Unet is designed from the perspective of extracting connections between local detailed features and making complements using long-distance dependency information, which is applied to the retinal vessel segmentation task. MTPA_Unet uses multi-resolution image input to enable the network to extract information at different levels. The proposed TPA module not only captures long-distance dependencies, but also focuses on the location information of the vessel pixels to facilitate capillary segmentation. The Transformer is combined with the convolutional neural network in a serial approach, and the original MSA module is replaced by the TPA module to achieve finer segmentation. Finally, the network model is evaluated and analyzed on three recognized retinal image datasets DRIVE, CHASE DB1, and STARE. The evaluation metrics were 0.9718, 0.9762, and 0.9773 for accuracy; 0.8410, 0.8437, and 0.8938 for sensitivity; and 0.8318, 0.8164, and 0.8557 for Dice coefficient. Compared with existing retinal image segmentation methods, the proposed method in this paper achieved better vessel segmentation in all of the publicly available fundus datasets tested performance and results.

Laser deposition (LD) is a useful repair technique and in-situ microstructure control is significant. However, the ability of in-situ microstructural control is inevitably limited in LD. Introducing induction heating into laser deposition is a potential solution for this challenging issue. In this research, the synchronous induction assisted laser deposition of Ti-6Al-4 V alloy were conducted and a thermal analysis model was established to investigate the influences of laser and induction energy on the morphology and size of the grains, the phase, and the Vickers hardness. Results showed that both of the thickness of equiaxed grain zone and the width of columnar grains increased with increasing laser energy and induction energy. The fraction of α phase increases with increasing induction energy due to sharply reduction of cooling rate during the cooling period caused by the post-heating, while the fraction of martensite increased with increasing laser energy. The “800 W-600A” and “1000 W-300A” samples have similar β grain morphology and size but consist of different phase, which is very distinctive from usual laser deposition process. This novel discovery indicates a great potential of microstructure control, the grains and the phase can be controlled separately by adjusting the laser parameters and induction parameters. Keywords: Synchronous induction assisted laser deposition, Ti-6Al-4 V alloy, Microstructure, Thermal behavior, Mathematical model analysis

This paper proposes a strategy to optimize the design of the substrate structures used in Additive Manufacturing (AM) by Directed Energy Deposition (DED) to minimize the residual stresses induced by this fabrication process. To this end, several numerical analyses were performed to analyse different substrate designs in order: (i) to reduce the sensitivity to the initial non-steady stage when the first layers of material are deposited, (ii) to optimize the heat flux through the substrate to reduce the Maximum Temperature Gradients (MTG) and, (iii) to modify the substrate stiffness and its mechanical constraining to the thermal deformations during the building process and the cooling phase. To ensure the reliability of the numerical simulations, an in-house software is calibrated to allow for an accurate analysis of DED. Thus, an experimental setting is undergone to feed the numerical model with suitable values of both material and process parameters through temperature and displacement measurements and numerical fitting. Once calibrated, the software is used to evaluate the performance of several substrate designs to mitigate the residual stresses induced by the DED process. A thin-walled rectangular part selected as industrial demonstrator showed a significant reduction (up to 62%) of the maximum tensile stresses.

Atherosclerosis is the basic pathological process of many diseases, such as coronary atherosclerosis and stroke. Nutrients can affect the occurrence and development of atherosclerosis. At present, in nutrition science, the research on atherosclerosis focuses on which nutrients play an important role in its prevention strategy, and what are the possible mechanisms of its action. In the current study, the process of atherosclerosis can be affected by adjusting the proportion of nutrients in the diet. In this review, we pay attention to the effects of phytosterols, omega-3-polyunsaturated fatty acids, polyphenol, vitamin, and other nutrients on atherosclerosis, pay attention to their current epidemiological status, current nutritional research results, and prevention or a possible mechanism to reduce the risk of development of atherosclerosis. So that more personalized nutritional approaches may be more effective in terms of nutritional intervention responses to atherosclerosis.

Background: There are several approaches that can be used for the pre-treatment identification of malnutrition in oncology populations including the Patient-Generated Subjective Global Assessment (PG-SGA), the 2015 consensus statement by the European Society for Clinical Nutrition and Metabolism (ESPEN 2015) and the Global Leadership Initiative on Malnutrition (GLIM).Aims: This study aimed to evaluate whether malnutrition, as defined by these three methods, can be used to predict complications in esophageal cancer (EC) patients after esophagectomy.Methods: We performed a single center, observational cohort study that included 360 EC patients undergoing esophagectomy from December 2014 to November 2019 at Daping Hospital in China. The prevalence of malnutrition in the study population was prospectively defined using the PG-SGA (≥9 defined malnutrition), and retrospectively defined using the ESPEN 2015 and the GLIM. The prevalence of malnutrition and association with postoperative complications were compared in parallel for the three methods.Results: The prevalence of malnutrition before surgery was 23.1% (83/360), 12.2% (44/360), and 33.3% (120/360) in the study population, as determined by the PG-SGA, the ESPEN 2015 and the GLIM, respectively. The PG-SGA and GLIM had higher diagnostic concordance (Kappa = 0.519, P < 0.001) compared to the ESPEN 2015 vs. GLIM (Kappa = 0.361, P < 0.001) and PG-SGA vs. ESPEN 2015 (Kappa = 0.297, P < 0.001). The overall incidence of postoperative complications for the study population was 58.1% (209/360). GLIM- and ESPEN 2015-defined malnutrition were both associated with the total number of postoperative complications in multivariable analyses. Moreover, GLIM-defined malnutrition exhibited the highest power to identify the incidence of complications among all independent predictors in a pooled analysis.Conclusion: Among the PG-SGA, the ESPEN 2015 and the GLIM, the GLIM framework defines the highest prevalence rate of malnutrition and appears to be the optimal method for predicting postoperative complications in EC patients undergoing esophagectomy. These results support the importance of preoperatively identifying malnutrition using appropriate assessment tools, because it can facilitate the selection of management strategies that will optimize the clinical outcomes of EC patients.

ObjectiveTransarterial chemoembolization (TACE) stands for an ideal therapy for patients with intermediate stage HCC. This study was carried out to observe the effect of microparticles-transarterial chemoembolization (microparticles-TACE, m-TACE) on the immune function of hepatocellular carcinoma (HCC) patients by detecting the proportion of regulatory (Treg) cells in the peripheral blood of HCC patients before and after m-TACE, and to determine whether m-TACE has a positive regulatory effect on the immune function of HCC patients.Methods33 HCC patients treated with Gelatn Sponge Microparticles (GSMs-TACE) were enrolled. Flow cytometry was used to determine the proportion of Treg cells and CD4+/CD8+ T cells in peripheral blood of HCC patients 1 day before GSMs-TACE, 1 to 2 weeks and 3 to 5 weeks after GSMs-TACE, respectively.ResultsThe Tregs cell proportion of HCC patients was significantly higher than that of the healthy and cirrhosis controls and was associated with various clinical indicators of HCC patients. The Treg cell proportion in HCC patients with BCLC stage C was higher than that of stage B patients; The Treg cell proportion at 1 to 2 weeks postoperatively was 8.54 ± 1.27%, which was significantly lower than that before the GSMs-TACE. The Treg cell proportion at 3 to 5 weeks postoperatively was 7.59 ± 1.27%, which continued to decline. The ratio of CD4+/CD8+ T cells was 1.31 ± 0.56, 1.86 ± 0.73, 1.76 ± 0.58% (P

An intact lung epithelial barrier is essential for lung homeostasis. The Na+, K+-ATPase (NKA), primarily serving as an ion transporter, also regulates epithelial barrier function via modulation of tight junctions. However, the underlying mechanism is not well understood. Here, we show that overexpression of the NKA β1 subunit upregulates the expression of tight junction proteins, leading to increased alveolar epithelial barrier function by an ion transport–independent mechanism. Using IP and mass spectrometry, we identified a number of unknown protein interactions of the β1 subunit, including a top candidate, myotonic dystrophy kinase–related cdc42-binding kinase α (MRCKα), which is a protein kinase known to regulate peripheral actin formation. Using a doxycycline-inducible gene expression system, we demonstrated that MRCKα and its downstream activation of myosin light chain is required for the regulation of alveolar barrier function by the NKA β1 subunit. Importantly, MRCKα is expressed in both human airways and alveoli and has reduced expression in patients with acute respiratory distress syndrome (ARDS), a lung illness that can be caused by multiple direct and indirect insults, including the infection of influenza virus and SARS-CoV-2. Our results have elucidated a potentially novel mechanism by which NKA regulates epithelial tight junctions and have identified potential drug targets for treating ARDS and other pulmonary diseases that are caused by barrier dysfunction.

A daily East Asia–Pacific teleconnection (EAP) index was constructed to investigate the impact of the intraseasonal variability (ISV) of this index on the genesis of multiple tropical cyclones (MTC) in boreal summer over the western North Pacific (WNP). The result indicates that the EAP index has dominant intraseasonal periods of 10–20 days, 20–40 days and 50–70 days, respectively. The ISV of the EAP during 1979–2019 can be classified into three types, a single-period-domination type (37%), a multiple period coexistence type (24%) and a transition type (39%). It is found that during El Niño (La Niña) summers, the ISV of the EAP is dominated by a higher-frequency (lower-frequency) oscillation with a period of around 20–30 (50–70) days. The distinctive ISV characteristics during the different ENSO years were accompanied with different dynamic and thermodynamic background conditions over the WNP and the South China Sea, which modulated the frequency and location of MTC genesis. By examining the relative contributions of individual environmental variables of the Genesis Potential Index, we found that the low-level absolute vorticity and mid-level relative humidity are two important environmental factors modulating MTC genesis. However, the relative role of these variables tends to change with the EAP ISV phase. The environmental condition over the SCS appears less influenced by ENSO. A more southern location of MTC genesis during El Niño is attributed to the change of the environmental humidity.

Against the backdrop of frequent food safety problems, the importance of establishing food traceability systems has become increasingly important and urgent to address the contradiction between consumer information on safe food choices and the proliferation of problematic foods. The purpose of this study is to empirically study the influencing factors of Chinese consumers on the food traceability system in the food safety field (hereinafter referred to as FTS). In this study, multiple models—push factor (information system success model), pull factor (ITM theory), mooring factor (TPB), and switching intention—were integrated into the push-pulling-mooring theory (PPM) to form a conceptual PPM comprehensive model framework to study the switching intentions of two-dimensional code traceability technology for dairy products of Chinese consumers. By collecting the questionnaire survey, 305 valid questionnaires were collected from the consumers of middle- and high-end dairy products in China, and the influencing factors of thrust, pull, and mooring force were identified. The results showed that 10 of the 11 hypotheses were positive, but the impact of perceived risk on user satisfaction was negative. The important value of this study is to conduct a comprehensive empirical analysis of the key factors influencing consumer choice of traceable safe food through an integrated multi-model framework to help identify ways to establish and improve consumer willingness to use QR code traceable system products, to increase consumer confidence in the use of traceable and safe food choices.

The building finish layer is a comprehensive structural system including the building exterior insulation system and building exterior finish. Combining with buildings has the advantage of reducing wall heat loss and building deformation caused by large temperature differences. Since the building finish layer is prone to cracking, hollowing, and peeling, during the application process, its safety needs to be studied and certified. This study prepares 20 groups of specimens, 15 anchor bolts in each group. The anchor bolt pull-out strength test is carried out. Anchoring damage evolution law and failure mode of anchor bolts are investigated. And the influence of anchoring methods on the pull-out bearing capacity is analyzed. In addition, ABAQUS finite element data simulation is carried out. The stress state of finish in thermomechanical coupling condition and without the effect of temperature are compared and analyzed. The influence factors of anchor bolt pull-out strength and the influence of temperature load on the long-term performance of building finish layer are obtained. The durability of the building finish layer is analyzed. The results show that the anchoring strength of the anchor bolt is positively correlated with the anchoring depth. The anchoring strength is influenced significantly by anchoring construction sequence and temperature. The stress under the coupled effect of temperature and load is greater than that of the single effect of load, and the stress distribution changes significantly. Due to thermal expansion and contraction, the anchor bolt would loosen, which is more prone to damage the building finish layer in a low temperature environment. The weight relationship of each influencing factor of the building finish layer is proposed. A systematic evaluation index system is established. The results of this study provide a basis for subsequent related research work and engineering applications.

Rapid population growth, climate change, intensive monoculture farming, and resource depletion are among the challenges that threaten the increasingly vulnerable global agri-food system. Heavy reliance on a few major crops is also linked to a monotonous diet, poor dietary habits, and micronutrient deficiencies, which are often associated with diet-related diseases. Diversification—of both agricultural production systems and diet—is a practical and sustainable approach to address these challenges and to improve global food and nutritional security. This strategy is aligned with the recommendations from the EAT-Lancet report, which highlighted the urgent need for increased consumption of plant-based foods to sustain population and planetary health. Bambara groundnut (Vigna subterranea (L.) Verdc.), an underutilized African legume, has the potential to contribute to improved food and nutrition security, while providing solutions for environmental sustainability and equity in food availability and affordability. This paper discusses the potential role of Bambara groundnut in diversifying agri-food systems and contributing to enhanced dietary and planetary sustainability, with emphasis on areas that span the value chain: from genetics, agroecology, nutrition, processing, and utilization, through to its socioeconomic potential. Bambara groundnut is a sustainable, low-cost source of complex carbohydrates, plant-based protein, unsaturated fatty acids, and essential minerals (magnesium, iron, zinc, and potassium), especially for those living in arid and semi-arid regions. As a legume, Bambara groundnut fixes atmospheric nitrogen to improve soil fertility. It is resilient to adverse environmental conditions and can yield on poor soil. Despite its impressive nutritional and agroecological profile, the potential of Bambara groundnut in improving the global food system is undermined by several factors, including resource limitation, knowledge gap, social stigma, and lack of policy incentives. Multiple research efforts to address these hurdles have led to a more promising outlook for Bambara groundnut; however, there is an urgent need to continue research to realize its full potential.

Soil organic matter (SOM) plays pivotal roles in characterizing dryland structure and function; however, remotely sensed spatially-detailed SOM mapping in these regions remains a challenge. Various digital soil mapping approaches based on either single-period remote sensing or spectral indices in other ecosystems usually produce inaccurate, poorly constrained estimates of dryland SOM. Here, a framework for spatially-detailed SOM mapping was proposed based on cross-wavelet transform (XWT) that exploits ecologically meaningful features from intra-annual fractional vegetation and soil-related endmember records. In this framework, paired green vegetation (GV) and soil-related endmembers (i.e., dark surface (DA), saline land (SA), sand land (SL)) sequences were adopted to extract 30 XWT features in temporally and spatially continuous domains of cross-wavelet spectrum. We then selected representative features as exploratory covariates for SOM mapping, integrated with four state-of-the-art machine learning approaches, i.e., ridge regression (RR), least squares-support vector machines (LS-SVM), random forests (RF), and gradient boosted regression trees (GBRT). The results reported that SOM maps from 13 coupled filtered XWT features and four machine learning approaches were consistent with soil-landscape knowledge, as evidenced by a spatially-detailed gradient from oasis to barren. This framework also presented more accurate and reliable results than arithmetically averaged features of intra-annual endmembers and existing datasets. Among the four approaches, both RF and GBRT were more appropriate in the XWT-based framework, showing superior accuracy, robustness, and lower uncertainty. The XWT synthetically characterized soil fertility from the consecutive structure of intra-annual vegetation and soil-related endmember sequences. Therefore, the proposed framework improved the understanding of SOM and land degradation neutrality, potentially leading to more sustainable management of dryland systems.

Background: As a public health emergency of international concern, the COVID-19 outbreak has had a tremendous impact on patients' psychological health. However, studies on psychological interventions in patients with COVID-19 are relatively rare.Objectives: This study examined the effectiveness of Cognitive Behavioral Therapy (CBT) in relieving patients' psychological distress during the COVID-19 epidemic.Methods: Ninety-three eligible participants selected by cluster sampling were randomized to an intervention group (N = 47) and a control group (N = 46). Participants in the control group received routine treatment according to the Chinese Management Guidelines for COVID-19, while participants in the intervention group received routine treatment with additional CBT. The Chinese Version of Depression Anxiety and Stress Scale-21 (DASS-21) was used to evaluate depression, anxiety, and stress for all participants at baseline and post-intervention. Two-sided t-test, and proportion tests were used to examine the differences between the intervention and control group for each DASS-21 indicator. Univariate linear regression was used to examine the association between chronic disease status and change in each DASS-21 indicator after intervention. Two-way scatter plots were generated to show the association of the length of hospital stay and the changes of each DASS-21 indicator by intervention and control groups.Results: Significant decreases in means were found for scales of depression, anxiety, stress and total DASS-21 in both intervention (p < 0.001) and control group (p = 0.001), with participants in the intervention group having a bigger reduction in means. After the intervention, more participants in the intervention group had no depression or anxiety symptoms than in the control group, but no statistical differences were found (p > 0.05). Compared with participants with chronic disease, participants with no chronic disease had a significantly larger reduction of total DASS-21 scale (coefficient = −4.74, 95% CI: −9.31; −0.17).The length of hospital stay was significantly associated with a greater increase in anxiety scale in the intervention group (p = 0.005), whilst no significant association was found in the control group (p = 0.29).Conclusions: The patients with COVID-19 experienced high levels of anxiety, depression and stress. Our study result highlights the effectiveness of CBT in improving the psychological health among patients with COVID-19, also suggests that CBT should be focused on patients with chronic disease and those who have longer hospital stays. These results have important implications in clinical practice in improving psychological health in the context of COVID-19 pandemic.Trial Registration: ISRCTN68675756. Available at: http://www.isrctn.com/ISRCTN68675756.

Bambara groundnut (BG, Vigna subterranea) and moringa (Moringa oleifera) are underutilized crops that improve nutritional quality of diets locally. The objectives of this study were to measure the performance of both crops on a marginal soil, analyze the harvested plant parts and noodles produced from them, and to undertake taste testing and survey consumer reactions. The noodles contained either 100% wheat flour or wheat with either BG flour (20%) or moringa powder (6%). BG yielded 0.70 t ha−1 of dry nuts and moringa 1.54 t ha−1 of dry leaflets. Both plant products were high in nutrients (especially K, and Ca in moringa) and some amino acids. Inclusion in noodles significantly enhanced their nutritional composition particularly energy content, crude fat, crude fiber and carbohydrate. Significant increases (p < 0.05) of Mg, Mn, P, K, and Zn occurred. Total essential amino acid concentration increased from 34.1% in the pure wheat noodles to 38.2% in BG and 34.8% in moringa noodles. Sensory analysis showed acceptability of all three noodle types was above the “Neither like nor dislike” category leaning toward the liking end for almost all the attributes tested. A consumer reaction survey showed there were internal and external factors motivating respondents to choose both products. Weak correlations were found between some of these factors and respondents' willingness to pay more for the BG and moringa noodles. We conclude that adopting a comprehensive research approach from plant to plate can assist transfer of underutilized crops from field studies to acceptable consumer products with enhanced nutritional profiles.

Symbiodiniaceae community structure in corals is crucial for understanding the plasticity of different holobionts under environmental stress. While this relies on molecular analyses, accuracy of molecular quantification, as influenced by DNA extraction efficiency and rDNA copy number variations in particular, has rarely been systematically investigated. Here, we report the development of a set of genus-specific qPCR assays. First, a protocol for efficient DNA isolation and accurate measurements of genome size and rDNA copy number was established. Second, seven newly designed genus-specific ITS2 primer sets were validated using computational and empirical analyses and qPCR assays were developed. We find that while the genome size ranges between 1.75 ± 0.21 and 4.5 ± 0.96 Gbp, rDNA copy number shows over 10-fold variation among Symbiodiniaceae species. Our protocol produced standard curves with high efficiencies (89.8–99.3%; R2 ≥ 0.999) and tight Cq values over different PCR conditions, illustrating high specificity and sensitivity of the qPCR assays. Tested on mock communities of mixed culture species, our qPCR results agreed well with microscopic counts and facilitated calibration of metabarcoding data. To test the applicability of our protocol for field samples, we analyzed three different Hong Kong coral samples. Six Symbiodiniaceae genera were detected in Acropora valida, Oulastrea crispata, and Platygyra acuta, with Breviolum, Effrenium, Fugacium, and Gerakladium sp. being reported for the first time. Our results suggest that aggressively disrupting cells to ensure thorough cell lysis, estimating cell loss and DNA loss, and validating qPCR assays are critical for success. The number of species examined here is limited, but the primers are potentially applicable to most species in respective genera, and the protocol and the approach to develop it provide a base and template toward a standardized procedure for quantitatively characterizing Symbiodiniaceae communities in corals.

Summary: Obesity commonly co-exists with fatty liver disease with increasing health burden worldwide. Family with Sequence Similarity 13, Member A (FAM13A) has been associated with lipid levels and fat mass by genome-wide association studies (GWAS). However, the function of FAM13A in maintaining metabolic homeostasis in vivo remains unclear. Here, we demonstrated that rs2276936 in this locus has allelic-enhancer activity in massively parallel reporter assays (MPRA) and reporter assay. The DNA region containing rs2276936 regulates expression of endogenous FAM13A in HepG2 cells. In vivo, Fam13a−/− mice are protected from high-fat diet (HFD)-induced fatty liver accompanied by increased insulin sensitivity and reduced glucose production in liver. Mechanistically, loss of Fam13a led to the activation of AMP-activated protein kinase (AMPK) and increased mitochondrial respiration in primary hepatocytes. These findings demonstrate that FAM13A mediates obesity-related dysregulation of lipid and glucose homeostasis. Targeting FAM13A might be a promising treatment of obesity and fatty liver disease. : Biological Sciences; Cell Biology; Functional Aspects of Cell Biology Subject Areas: Biological Sciences, Cell Biology, Functional Aspects of Cell Biology

Objectives This study aimed to explore the effects of astragaloside IV on metabolic syndrome induced by a high-fructose/high-fat diet in rats. Methods Rats were randomized into four groups: normal control, metabolic syndrome, metabolic syndrome + intraperitoneal astragaloside 0.5 mg/kg/day, and metabolic syndrome + intraperitoneal astragaloside 2.0 mg/kg/day (n=30 per group) for 14 continuous days. Left ventricular functions were evaluated by hemodynamic and echocardiographic parameters. Results Metabolic syndrome rats had a thicker interventricular septum and left ventricular posterior wall, accompanied by a higher E/A wave ratio, reduced E′ wave, increased A′ wave, decreased E′/A′ wave ratio, and higher E/E′ wave ratio. Astragaloside decreased insulin and triglyceride levels and improved diastolic dysfunction with no effects on systolic function. A high-fructose/high-fat diet also increased oxidative stress and decreased the myocardial endothelial nitric oxide synthase (NOS) dimer ratio, thus impairing nitric oxide (NO) production and reducing cyclic guanosine monophosphate (cGMP) production. Astragaloside increased NO and cGMP production in the myocardium and improved diastolic function. Conclusions Astragaloside alleviated oxidative stress and restored NO signaling, thus improving myocardial left ventricular diastolic dysfunction in rats with metabolic syndrome. The underlying mechanisms could be associated with alleviation of oxidative stress and activation of the endothelial NOS/NO/cGMP pathway.

Brown corundum dust ash(BCFA) is an industrial solid waste from the brown corundum production process. The alkali metal is a key factor limiting its comprehensive use. Currently, BCFA is mainly stockpiled and occupies a large amount of land. Its fine particles are easy to cause air pollution. The addition of NH _4 HSO _4 allows for the effective extraction of K from BCFA and the efficient use of BCFA. Under optimum conditions: NH _4 HSO _4 to BCFA mass ratio of 1.2:1, the roasting temperature of 240 °C, roasting time of 2 h, water leaching time of 90 min, water leaching temperature of 65 °C water leaching liquid to solid ratio of 3:1, the leaching rate of K from BCFA reached 97%. The leachate was crystallised by evaporation to obtain K, N compound fertiliser for agricultural use. The leaching residue is mainly Al and Si, which can be used for the preparation of refractory materials, aluminium and silicon molecular sieves, construction materials and other raw materials.

The fungal pathogen Candida albicans is diploid, which hinders genome-wide studies. Here, Gao et al. present a piggyBac transposon-mediated mutagenesis system using stable haploid C. albicans strains, and use it to identify genes and mechanisms underlying azole resistance.

Herein, we report that the ternary chalcogenide nanosheet exhibits different affinity toward oligonucleotides with different lengths and efficiently quenches the fluorescence of dye-labeled DNA probes. Based on these findings, as a proof-of-concept application, the ternary chalcogenide nanosheet is used as a target cyclic amplification biosensor, showing high specificity in discriminating single-base mismatch. This simple strategy is fast and sensitive for the single nucleotide polymorphism detection. Ultralow detection limit of unlabeled target (250 fM) and high discrimination ratio (5%) in the mixture of perfect match (mutant-type) and single-base mismatch (wild-type) target are achieved. This sensing method is extensively compatible for the single nucleotide polymorphism detection in clinical samples, making it a promising tool for the mutation-based clinical diagnostic and genomic research.

Abstract Background Obesity is a well-established risk factor for atherosclerosis. However, it is unknown which measure of adiposity best relates to atherosclerosis in relatively lean maintenance hemodialysis (MHD) patients. We aimed to explore and compare the associations between different adiposity indices reflecting general, abdominal, visceral adiposity and arteriosclerosis risk with atherogenic index of plasma(AIP) in relatively lean MHD patients. Methods We conducted a multicenter, cross-sectional study in Guizhou Province, Southwest China. General/abdominal adiposity indices like body mass index (BMI), waist circumference(WC), waist-height ratio(WHtR), conicity index (Ci) and visceral obesity indices including visceral adiposity index (VAI), lipid accumulation product (LAP) and the hypertriglyceridemic waist phenotype (HW phenotype) were recorded. Univariate and multivariate linear regression models were used. Results All adiposity indices correlated positively with AIP in univariate analysis both in men and women except for Ci. After adjustment for age and traditional atherosclerosis risk factors, BMI, WC, WHtR, VAI and LAP still had associations with AIP both in men (β = 0.265, 0.153, 0.16, 0.788 and 0.74, respectively, all P

Abstract Arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C) is a genetic cardiac muscle disease that accounts for approximately 30% sudden cardiac death in young adults. The Ser358Leu mutation of transmembrane protein 43 (TMEM43) was commonly identified in the patients of highly lethal and fully penetrant ARVD subtype, ARVD5. Here, we generated TMEM43 S358L mouse to explore the underlying mechanism. This mouse strain showed the classic pathologies of ARVD patients, including structural abnormalities and cardiac fibrofatty. TMEM43 S358L mutation led to hyper-activated nuclear factor κB (NF-κB) activation in heart tissues and primary cardiomyocyte cells. Importantly, this hyper activation of NF-κB directly drove the expression of pro-fibrotic gene, transforming growth factor beta (TGFβ1), and enhanced downstream signal, indicating that TMEM43 S358L mutation up-regulates NF-κB-TGFβ signal cascade during ARVD cardiac fibrosis. Our study partially reveals the regulatory mechanism of ARVD development.

A three-layer Ta2O5-containing coating was successfully fabricated by laser cladding on a pure Ta substrate. The maximum thickness of such a coating is about 1.6 mm. The microstructure, phase constitution and elemental distribution in the coating were investigated. Results show that the coating has been metallurgically bonded to the Ta substrate and the microstructure exhibits a graded change along the deposition direction from Ta substrate to the top of coating. In the layers I and II of the graded coating, the microstructure evolution can be confirmed as a result of hypomonotectic reaction, but in the layer Ⅲ it was formed by hypermonotectic reaction. At the top of coating, the microstructure was still homogeneous although liquid phase separation had occurred,which can be attributed to the fact that the O-rich droplets do not have enough time to float at high cooling rate.The theoretical calculation results show that during laser cladding, the solidification time of the melt pool was less than 0.1 s, which fits well with the results from the experiment.

Abstract Background Although adult human tissue-derived epidermal stem cells are capable of differentiating into enamel-secreting ameloblasts and forming teeth with regenerated enamel when recombined with mouse dental mesenchyme that possesses odontogenic potential, the induction rate is relatively low. In addition, whether the regenerated enamel retains a running pattern of prism identical to and acquires mechanical properties comparable with human enamel indeed warrants further study. Methods Cultured human keratinocyte stem cells (hKSCs) were treated with fibroblast growth factor 8 (FGF8) and Sonic hedgehog (SHH) for 18 h or 36 h prior to being recombined with E13.5 mouse dental mesenchyme with implantation of FGF8 and SHH-soaked agarose beads into reconstructed chimeric tooth germs. Recombinant tooth germs were subjected to kidney capsule culture in nude mice. Harvested samples at various time points were processed for histological, immunohistochemical, TUNEL, and western blot analysis. Scanning electronic microscopy and a nanoindentation test were further employed to analyze the prism running pattern and mechanical properties of the regenerated enamel. Results Treatment of hKSCs with both FGF8 and SHH prior to tissue recombination greatly enhanced the rate of tooth-like structure formation to about 70%. FGF8 and SHH dramatically enhanced stemness of cultured hKSCs. Scanning electron microscopic analysis revealed the running pattern of intact prisms of regenerated enamel is similar to that of human enamel. The nanoindentation test indicated that, although much softer than human child and adult mouse enamel, mechanical properties of the regenerated enamel improved as the culture time was extended. Conclusions Application of FGF8 and SHH proteins in cultured hKSCs improves stemness but does not facilitate odontogenic fate of hKSCs, resulting in an enhanced efficiency of ameloblastic differentiation of hKSCs and tooth formation in human–mouse chimeric tooth germs.

Using the array technique, the multi-channel synthetic aperture radar (SAR) has the capability of high-resolution imaging, detection, and location of the ground moving targets, which is a powerful tool during the remote sensing of smart city. Recently, the theory of compressive sensing has been applied to radar imaging with data of compressive sampling, which can effectively reduce the burden of current radar system. In this paper, we focus on SAR moving targets imaging from sparse aperture (SA) data with accurate target motion compensation. The procedure of motion compensation is decomposed into two steps: the SPECAN processing in the range frequency and azimuth time domain is first applied and then followed by the residue component correction embedded into sparse imaging. To overcome the SA and target motion, a novel parametric sparse imaging approach is proposed by addressing the problems of Doppler ambiguity and phase errors. In the scheme, a parametric and dynamic dictionary is used to include these two important issues. Then, a modified orthogonal matching pursuit method is presented for high-quality imaging with Doppler ambiguity number estimation and phase error correction, which can deal with the case of multiple moving targets. Finally, experimental analysis is performed to confirm the effectiveness of the proposed algorithm.

Cold and freezing stress is one of the most harmful environmental stresses, especially in temperate and subtropical areas, that adversely affects plant growth, development, and yield production. Betula platyphylla Sukaczev, also known as white birch, is one of the most valuable, important, and widely distributed tree species in East Asia. This study explored the effects of cold acclimation (CA) in reducing the destructive effect of freezing stress in B. platyphylla seedlings. We measured the physiological and biochemical characteristics of B. platyphylla seedlings, such as chlorophyll content, electrolyte leakage (EL), malondialdehyde (MDA), antioxidant enzymes (such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)), and proline content before and after freezing stress to observe the contribution of CA in reducing the detrimental effects of freezing stress. The results showed that CA increased physiological and biochemical characteristics of B. platyphylla seedlings before and after freezing stress, except for chlorophyll content. Antioxidant enzymes were significantly positively correlated with proline, MDA, and EL content, and negatively correlated with chlorophyll content. Moreover, histochemical detection (H2O2 and O2−) and cell death were revealed to be induced by cold stress in B. platyphylla seedlings. Furthermore, it was revealed that increased time and decreased temperature of the CA process significantly influenced the physiological and biochemical parameters. Overall, the CA process significantly reduced the detrimental effects of freezing stress compared to the control treatment in B. platyphylla seedlings. Taken together, these findings provide beneficial information toward understanding the mechanism of CA and freezing stress in B. platyphylla. Furthermore, the substantial activity of physiological and biochemical results could be used as selection criteria for screening time and temperature points of cold/freezing stress in further omics analyses. In addition, the combination of current study results, further omics analyses, and genetic engineering techniques directly contribute to sustainable forest management systems, tree plantations, and conservation of tree species, especially non-cold/non-freezing tolerant tree species.

Residual stresses are one of the primary causes for the failure of parts or systems in metal additive manufacturing (AM), since they easily induce crack propagation and structural distortion. Although the formation of residual stresses has been extensively studied, the core factors steering their development in AM have not been completely uncovered. To date, several strategies based on reducing the thermal gradients have been developed to mitigate the manifestation of residual stresses in AM; however, how to choose the optimal processing plan is still unclear for AM designers. In this regard, the concept of the yield temperature, related to the thermal deformation and the mechanical constraint, plays a crucial role for controlling the residual stresses, but it has not been duly investigated, and the corresponding approach to control stresses is also yet lacking. To undertake such study, a three-bar model is firstly used to illustrate the formation mechanism of the residual stress and its key causes. Next, an experimentally calibrated thermomechanical finite element model is used to analyze the sensitivity of the residual stresses to the scan pattern, preheating, energy density, and the part geometry and size, as well as the substrate constraints. Based on the numerical results obtained from this analysis, recommendations on how to minimize the residual stresses during the AM process are provided.

Successive flashover would result in carbonized tracking on insulator surface and cause deterioration to the insulation. Thus, investigation of the tracking can be beneficial in understanding flashover characteristics during long-term operation. In this paper, DC flashover was operated on the insulator, and the image of tracking after successive discharge were captured. Improved differential box-counting method (IDBM) was applied to analyze these images based on fractal theory. Weighted item was suggested during the counting procedure for rectangle image with margin covered by cut-size box. Fractal dimension of the tracking was calculated according to the suggested method. It is claimed that the suggested method could estimate the discharge propagation property and deterioration characteristics on the insulator surface. Moreover, IDBM showed advantages in image pre-processing and deterioration property revealed compared to traditional box-counting method attributing to the consideration of color depth. This image analysis method shows universality in dealing with tracking image and could provide additional information to flashover voltage. This paper suggested a potential approach for the investigation of discharge mechanism and corresponding deterioration in future research.

As an effective optical diagnosis method, tunable diode-laser absorption spectroscopy (TDLAS) has increasingly moved to examine nonuniform flows, such as two-dimensional combustion diagnosis. To investigate the effect of nonuniformity along the line of sight in a measurement using TDLAS, the integrated absorbance (IA, the key intermediate quantity in TDLAS) error was quantified. The error distribution is obtained from the line-shape parameters through the comprehensive analysis of the line-shape function and the fitting method. The effects of the fitting function and the absorption line overlap are also considered. A general method for estimating the error is given. The work illustrates the applicability of TDLAS technology in nonuniform flow fields and provides input parameters for the evaluation of tunable diode laser absorption tomography error.

Free-living (FL) and particle-associated (PA) bacterioplankton communities play critical roles in biogeochemical cycles in the ocean. However, their community composition, assembly process and functions in the continental shelf and slope regions are poorly understood. Based on 16S rRNA gene amplicon sequencing, we investigated bacterial communities’ driving factors, assembly processes and functional potentials at a subtropical marginal sea. The bacterioplankton community showed specific distribution patterns with respect to lifestyle (free living vs. particle associated), habitat (slope vs. shelf) and depth (surface vs. DCM and Bottom). Salinity and water temperature were the key factors modulating turnover in the FL community, whereas nitrite, silicate and phosphate were the key factors for the PA community. Model analyses revealed that stochastic processes outweighed deterministic processes and had stronger influences on PA than FL. Homogeneous selection (Hos) was more responsible for the assembly and turnover of FL, while drift and dispersal limitation contributed more to the assembly of PA. Importantly, the primary contributor to Hos in PA was Gammaproteobacteria:Others, whereas that in FL was Cyanobacteria:Bin6. Finally, the PICRUSt2 analysis indicated that the potential metabolisms of carbohydrates, cofactors, amino acids, terpenoids, polyketides, lipids and antibiotic resistance were markedly enriched in PA than FL.

The temperature properties of real-type direct-current gas-insulated transmission lines (DC-GIL) with a basin-type spacer were investigated by the finite element method in this paper. A horizontally installed model was established and the temperature distribution was obtained with a 3D model. The specific heat capacity and thermal conductivity of the spacer were measured and applied in the simulation. The results show that the temperature of the convex surface was slightly higher than that of the concave surface. With an increase in the SF6 pressure, the temperature of the spacer decreased, which can be attributed to the improvement of convection due to increases in the heat capacity per unit volume. With an increase in the ambient temperature, the temperature of the spacer increased linearly. The temperature difference between the inner and outer parts of the spacer increased with increases in the load current. Besides, an obvious increase in the surface electric field strength appeared under the influence of the thermal gradient compared to the results without the thermal gradient. Thus, special attention should be paid to the insulation properties of the spacer considering the influence of temperature distribution. This study evaluates both the thermal and insulation characteristics of the GIL along with the spacer under various conditions.

Zero liquid discharge (ZLD) aims to minimize liquid waste generation whilst extend water supply, and this industrial strategy has attracted renewed interest worldwide in recent years. In spite of the advantages such as reduced water pollution and resource recovery from waste, there are several challenges to overcome prior to wider applications of ZLD. This study will examine the main processes involved in ZLD, and analyze their limitations and potential solutions. This study also differs from past reviews on the subject, by providing a summary of the challenges that were found light of in prevalent studies. To fulfill the sustainable vision, future research that can bridge the gap between the theoretical study and industrial practice is highlighted.

The OH-initiated reaction of ethylbenzene results in major OH addition, and the formed ethylbenzene-OH adducts subsequently react with O2 and NO2, which determine the components of the oxidation products. In this study, nine possible reaction paths of the most stable ethylbenzene-OH adduct, EB-Ortho (2-ethyl-hydroxycyclohexadienyl radical intermediate), with O2 and NO2 were studied using density functional theory and conventional transition state theory. The calculated results showed that ethyl-phenol formed via hydrogen abstraction was the major product of the EB-Ortho reaction with O2 under atmospheric conditions. Peroxy radicals generated from O2 added to EB-Ortho could subsequently react with NO and O2 to produce 5-ethyl-6-oxo-2,4-hexadienal, furan, and ethyl-glyoxal, respectively. However, nitro-ethylbenzene formed from NO2 addition to EB-Ortho was the predominant product of the EB-Ortho reaction with NO2 at room temperature. The total calculated rate constant of the EB-Ortho reaction with O2 and NO2 was 9.57 × 10−16 and 1.78 × 10−11 cm3 molecule−1 s−1, respectively, approximately equivalent to the experimental rate constants of toluene-OH adduct reactions with O2 and NO2. This study might provide a useful theoretical basis for interpreting the oxygen-containing and nitrogen-containing organics in anthropogenic secondary organic aerosol particles.

In this study, the removal of 2,4-dichlorophenol (2,4-DCP) from aqueous solutions was performed using coconut shell activated carbon prepared by a two-stage self-generated atmosphere method. Coconut shell was first semi-carbonized at 300 °C for an hour followed by chemical activation with zinc chloride (ZnCl2) as activating agent at 500 °C for 2 h. The effect of impregnation ratio on the physical and chemical properties of activated carbons (ACs) was studied. The morphology and surface chemistry of the prepared carbons were characterized using Scanning Electron Microscope (SEM) and Fourier Transform Infrared spectroscopy (FTIR) respectively. The percentage of yield for the prepared AC was found to be in the range of 26.40 – 38.82 %. AC registered the highest adsorption capacity and was used in subsequent batch adsorption studies consisting of parameters such as initial concentration, adsorbent dosage and solution pH. The maximum surface area of the best produced AC was recorded as 1482 m2 g-1. The adsorption capacity was found to increase in proportional to the initial concentration and adsorbent dosage, while acidic solution pH was more favourable for the adsorption of 2,4-DCP by the prepared AC. The equilibrium time for the adsorption of 20 mg L-1 of 2,4-DCP on 0.5 g of AC was achieved in 180 min. Adsorption isotherms such as Langmuir, Freundlich and Temkin isotherm models were employed to examine the experimental isotherms while the reaction kinetic data was analysed using pseudo-first-order, pseudo-second-order and intraparticle diffusion model. The 2,4-DCP adsorption results fitted best in the Freundlich isotherm as indicated by the high correlation coefficient value (R2 > 0.9949) while the adsorption kinetic fitted to the pseudo second-order model (R2 > 0.9621).DOI: http://dx.doi.org/10.5755/j01.ms.23.4.16221

Abstract Background Thymosin β4 (Tβ4) is closely associated with the cytoskeleton, inflammation, wound healing, angiogenesis, apoptosis, and myocardial regeneration, but the effects of Tβ4 treatment on chronic renal tubular interstitial fibrosis (CRTIF) are poorly known. This study aimed to examine the effects of Tβ4 on the renal apoptosis and the expression of transforming growth factor (TGF-β), E-cadherin, and α-smooth muscle actin (α-SMA) in CRTIF rat models. Methods Male SD rats were randomized into four groups (sham group, unilateral ureteral obstruction (UUO) group, UUO + low-dose Tβ4 group, and UUO + high-dose Tβ4 group). The pathological changes of kidney tissue and its function were assessed two weeks after UUO. In renal interstitial tissue,TGF-β, E-cadherin and α-SMA expression was detected by western blot. In tubular epithelial cells, E-cadherin and α-SMA expression was detected using Real-time qPCR and western blot. Cell apoptosis of rat renal interstitial tissue and tubular epithelial cells was evaluated by immunofluorescence and western blot. Results Two weeks after UUO, no differences in blood urea nitrogen and creatinine were observed between the four groups (P > 0.05). Compared to the UUO group, Tβ4 treatment decreased the 24-h proteinuria (P

Background/Aims: Krüppel-like factor (KLF) 7 protein is a member of the KLF transcription factor family, which plays important roles in regulating the expression of genes involved in cell growth, proliferation, differentiation and metabolism. However, the role of KLF7 in gastric cancer (GC) is unknown. The aim of this study is to explore the role of KLF7 in GC and its correlation with clinicopathological characteristics and prognosis of GC patients. Methods: We first systematically evaluated dysregulation of the KLF family in The Cancer Genome Atlas (TCGA) GC database. Then, 252 patients who underwent surgery for GC were enrolled to validate the results from the TCGA. Functional studies were also used to explore the role of KLF7 in GC. Results: In the TCGA database, we found that KLF7 was an independent predictor for survival by both univariate and multivariate analysis (P

Abstract Most risk analysis models systematically underestimate the probability and impact of catastrophic events (e.g., economic crises, natural disasters, and terrorism) by not taking into account interconnectivity and interdependence of risks. To address this weakness, we propose the Cascading Alternating Renewal Process (CARP) to forecast interconnected global risks. However, assessments of the model’s prediction precision are limited by lack of sufficient ground truth data. Here, we establish prediction precision as a function of input data size by using alternative long ground truth data generated by simulations of the CARP model with known parameters. We illustrate the approach on a model of fires in artificial cities assembled from basic city blocks with diverse housing. The results confirm that parameter recovery variance exhibits power law decay as a function of the length of available ground truth data. Using CARP, we also demonstrate estimation using a disparate dataset that also has dependencies: real-world prediction precision for the global risk model based on the World Economic Forum Global Risk Report. We conclude that the CARP model is an efficient method for predicting catastrophic cascading events with potential applications to emerging local and global interconnected risks.

Abstract Backgroud Mycoplasma synoviae (M. synoviae) is widely distributed around the world, and leads to serious economic losses in the world every year. Nevertheless, the incidence and epidemiology of M. synoviae infection in China have remained unclear. Results In this study we demonstrate that over 9773 broiler chicken flocks in 16 Chinese provinces were affected by M. synoviae between 2010 and 2015. Our epidemiological study revealed that M. synoviae was widely prevalent in multi-aged Chinese native breeder chickens, and the prevalence of M. synoviae in embryos of breeders reached up to 16.29%. In addition, our data showed that chickens aged 14 days or younger carried simultaneously high levels of maternal antibody against M. synoviae and high M. synoviae infection (10%), and low M. synoviae antibody levels in breeders and high proportion of M. synoviae infection in embryos could increase the chances of incidence in the offspring. Finally, our results also indicated that 3- to 7-week-old chickens might be most the susceptible to M. synoviae and, therefore, might play a key role in the horizontal transmission of M. synoviae. Conclusion Our findings suggest that M. synoviae is widely circulating in Chinese native chickens, accordingly, effective control measures are urgently needed to control the spread.

Abstract It is well known that the Mathieu series has a wide application in mathematics science. In this paper, we use the elementary method and construct some new inequalities to study the computational problem of the partial reciprocal sums related to the Mathieu series and obtain an interesting inequality and a related identity.

In this study, we selected 9 typical coal samples with different metamorphic grades as the study subjects, measured their initial 30-min gas desorption at 30 °C and different pressure using a self-developed gas adsorption/desorption device. Based on the characteristics of gas desorption from coal samples, we proposed a direct fitting method for measurement of gas content in coalbed, analyzed the effects of sampling time on the measurement results and determined the reasonable sampling time of coal samples with different metamorphic grades at different gas adsorption pressure at equilibrium. The results show that (1) the error of gas contents obtained using the direct fitting method relative to that obtained using indirect method is less than 10%, which meets the actual on-site requirements and verifies the feasibility of the direct fitting method; (2) when the relative error is controlled within ±10%, the reasonable sampling time of coal samples is linearly related to the gas adsorption pressure at equilibrium; (3) the reasonable sampling time of coal samples with the same metamorphic grade exhibits a shortening trend with increasing gas adsorption pressure at equilibrium; (4) for coal samples with similar gas adsorption pressure at equilibrium, the reasonable sampling time of coal samples displays a shortening trend with increasing metamorphic grade. Overall, the study provides a basis for improving the measurement accuracy of gas content in coalbed. Keywords: Gas content, Fitting, Desorption, Metamorphic grade, Sampling time

The mechanical anisotropy of laser directed energy deposition (L-DED) titanium alloys using laser solid forming (LSF) is significantly deteriorated owing to the coarse prior β columnar grains and continuous grain boundary α-laths (αGB). The influence mechanisms of trace boron (up to 0.25 wt%) on the microstructure, tensile properties and anisotropy of Ti6Al4V manufactured by LSF were investigated. The sizes of β grains and α phases both decreased with increasing boron content. Compared with the Ti6Al4V deposit, when the addition of boron was 0.08 wt%, the average width of β grains was reduced by about one order of magnitude owing to the growth restricting effect caused by boron and Zener pinning by TiB. Besides, the average length and aspect ratio of α-laths were reduced by 43% and 33%, respectively, owing to the refinement of β grains and the heterogeneous nucleation of α phases on TiB particles. With an increase in boron content, the strength anisotropy gradually decreased for the disappearance of αGB, the elongation anisotropy initially increased and then significantly decreased for the poor deformation compatibility between the TiB and the matrix. A better combination of strength and elongation could be obtained with the addition of 0.08 wt% boron to Ti6Al4V. Keywords: Laser deposition, Titanium alloys, Microstructure, Mechanical properties, Anisotropy

In recent years, as environmental protection requirements have expanded, SF6/N2 mixed gases have been selected as an insulating medium for high-voltage equipment such as GIL (gas insulated transmission line) without undertaking breaking tasks, which is superior in terms of insulation, economy and environmental protection. The development of streamers and the two-dimensional distribution of positive and negative ions over time in an SF6/N2 mixed gas system are shown in this research. The SF6/N2 mixed gas is adopted as the research object, and the discharge model for a needle-plate electrode under mixed gas conditions is built. COMSOL Multiphysics is used to develop a fluid dynamics model for the SF6/N2 mixed gas discharge in a non-uniform field. The spatio-temporal evolution processes for the electron, positive ion and negative ion densities and the electric field strength during discharge are obtained through numerical simulations. At the same time, the influence of the voltage amplitude and mixed gas ratio on the development process of streamers is studied. The presented simulation results can provide a certain microscopic interpretation for the needle-plate discharge defects that appear in power equipment using SF6/N2 mixed gas.

Diatoms and dinoflagellates dominate coastal marine phytoplankton communities as major players of marine biogeochemical cycles and their seasonal succession often leads to harmful algal blooms (HABs). What regulates their respective dominances and the development of the HABs remains elusive. Here we conducted time-sequential metatranscriptomic profiling on a natural assemblage that evolved from diatom dominance to a dinoflagellate bloom to interrogate the underlying major metabolic and ecological drivers. Data reveals similarity between diatoms and dinoflagellates in exhibiting high capacities of energy production, nutrient acquisition, and stress protection in their respective dominance stages. The diatom-to-dinoflagellate succession coincided with an increase in turbidity and sharp declines in silicate and phosphate availability, concomitant with the transcriptomic shift from expression of silicate uptake and urea utilization genes in diatoms to that of genes for light harvesting, diversified phosphorus acquisition and autophagy-based internal nutrient recycling in dinoflagellates. Furthermore, the diatom-dominant community featured strong potential to carbohydrate metabolism and a strikingly high expression of trypsin potentially promoting frustule building. In contrast, the dinoflagellate bloom featured elevated expression of xanthorhodopsin, and antimicrobial defensin genes, indicating potential importance of energy harnessing and microbial defense in bloom development. This study sheds light on mechanisms potentially governing diatom- and dinoflagellate-dominance and regulating bloom development in the natural environment and raises new questions to be addressed in future studies.

Scaffold proteins are defined as pivotal molecules that connect upstream receptors to specific effector molecules. Caspase recruitment domain protein 10 (CARD10) gene encodes a scaffold protein CARMA3, belongs to the family of CARD and membrane-associated guanylate kinase-like protein (CARMA). During the past decade, investigating the function of CARMA3 has revealed that it forms a complex with BCL10 and MALT1 to mediate different receptors-dependent signaling, including GPCR and EGFR, leading to activation of the transcription factor NF-κB. More recently, CARMA3 and its partners are also reported to be involved in antiviral innate immune response and DNA damage response. In this review, we summarize the biology of CARMA3 in multiple receptor-induced NF-κB signaling. Especially, we focus on discussing the function of CARMA3 in regulating NF-κB activation and antiviral IFN signaling in the context of recent progress in the field.