Search

Interstitial-free (IF) steel with ultrafine-grained (UFG) size was used to clarify the underlying mechanisms of the ‘extra-hardening’ behavior from the Hall-Petch plot and the discontinuous yielding behavior from axial tensile tests. Using transmission electron microscopy (TEM) at different tensile strains, it was found that the TEM micrographs for the bulk sample of the UFG IF steel showed a decrease in dislocation density in the grain interior after experiencing a micro tensile strain of 1.0% before macro-yielding. Additionally, an increase in dislocation density was found in the grain interior, with a micro tensile strain of 15% in the necking region after macro-yielding. In-situ compression tests in a TEM for micropillars were performed to directly observe the motion of dislocations and corresponding interactions with grain boundaries in UFG IF steel. Before macro-yielding, pre-existing dislocations in the grain interior moved toward the grain boundary, wherein they were then absorbed. The dislocation density decreased significantly due to the annihilation, which was accompanied by an increase in load during pre-yielding. The decrease in dislocation density in the grain interior of the micropillar is consistent with the results obtained from the bulk sample. After macro-yielding, the burst-like dislocation emission emerged from the grain boundary, leading to a yield drop and a discontinuous yielding behavior. Furthermore, the higher yield stress and the yield drop in the UFG IF steel can be understood by the decrease in dislocation density according to a combination of the Orowan model as well as the Johnston-Gilman model.

Biochar additions to agricultural soil have been shown to result in numerous potential benefits; however, most studies have been conducted in greenhouse or laboratory trials with few being conducted in the field and particularly in association with organic farming systems. Herein, we address this gap by conducting on-farm studies on the efficacy of locally produced biochar as a soil amendment in small-scale organic agriculture on ten farms in San Juan County, WA. Biochar produced from local timber harvest residues in the San Juan Islands was applied in factorial combination with a poultry litter based fertilizer to replicated plots on all ten farms. Dry beans (Phaseolus vulgaris L.) were grown on eight of the farms with green beans and cauliflower being grown on the other two. Soils were examined for nitrogen (N), phosphorus (P), and carbon (C) pools during the growing season. Dry bean samples were evaluated for metal and nutrient uptake. Biochar additions increased soil total C by 32–33%, soil available NH4+ by 45–54% through mid-season, pontentially mineralizable N by 48–110%, and citrate extractable P by 29%; biochar additions enhanced soil NO3−-N, NH4+-N, and P retention in the rooting zone by 33%, 53% and 39% respectively. Increased availability of soil P, Fe, Mg, Zn was reflected in the nutrient concentration of harvested dry beans. Our study demonstrates that locally produced wood biochar has the potential to increase soil nutrient availability and uptake. By producing biochar from timber harvest residues and applying them on neighboring organic farms on the San Juan Islands, WA, this study leveraged the local resources and community readiness to drive forest restoration and sustainable agricultural practices in addition to the demonstrated potential short-term benefits of biochar additions for organic farms on the sandy soils of the San Juan Islands.

Turmeric is commonly used as a medicinal herb and dietary supplement. Its active ingredient, curcumin, has been shown to possess antitumor effects in colorectal cancer patients. However, poor absorption of curcumin in intestine impedes its wide clinical application. Our previous findings showed that the presence of turmerones increased the accumulation of curcumin inside colonic cells. Hence, we hypothesized that curcumin with turmerones or present in turmeric ethanolic extract would augment its anti-tumor activities in tumor-bearing mice. The pharmacokinetics of curcumin in different preparations (containing same amount of curcumin) were studied in mice. The anti-tumor efficacies of curcumin or turmeric extract (with absorbable curcumin) in combination with bevacizumab were further investigated in HT29 colon tumor-bearing mice. Pharmacokinetic results showed that the plasma curcumin level of turmeric extract-fed mice was the highest, suggesting turmeric extract had the best bioavailability of curcumin. Besides, combined turmeric extract plus bevacizumab treatment significantly inhibited the tumor growth. Such inhibitory effects were stronger than those of curcumin plus bevacizumab or bevacizumab alone and were comparable with those of 5-fluorouracil+leucovorin+oxaliplatin (FOLFOX) plus bevacizumab. Notably, there was no observable side effect induced by turmeric extract treatment while significant side effects were found in FOLFOX-treated mice. In conclusion, combination of turmeric extract with bevacizumab possessed potent anti-tumor effects without observable side effects, strongly suggesting the adjuvant use of turmeric extract in colorectal cancer therapy. Our current findings warrant the confirmation regarding the benefits arising from the combined use of bevacizumab and turmeric in colorectal cancer patients in the near future.

Transcription factors (TFs) play critical roles in mediating defense of plants to abiotic stresses through regulating downstream defensive genes. In this study, a wheat C2H2-ZFP (zinc finger protein) type TF gene designated as TaZAT8 was functionally characterized in mediating tolerance to the inorganic phosphate (Pi)-starvation stress. TaZAT8 bears conserved motifs harboring in the C2H2-ZFP type counterparts across vascular plant species. The expression of TaZAT8 was shown to be induced in roots upon Pi deprivation, with a Pi concentration- and temporal-dependent manner. Overexpression of TaZAT8 in tobacco conferred plants improved tolerance to Pi deprivation; the transgenic lines exhibited enlarged phenotype and elevated biomass and phosphorus (P) accumulation relative to wild-type (WT) after Pi-starvation treatment. NtPT1 and NtPT2, the tobacco phosphate transporter (PT) genes, showed increased transcripts in the Pi-deprived transgenic lines, indicative of their transcriptional regulation by TaZAT8. Overexpression analysis of these PT genes validated their function in mediating Pi acquisition under the Pi deprivation conditions. Additionally, the TaZAT8-overexpressing lines also behaved enhanced antioxidant enzyme (AE) activities and enlarged root system architecture (RSA) with respect to WT. Evaluation of the transcript abundance of tobacco genes encoding AE and PIN proteins, including NtMnSOD1, NtSOD1, NtPOD1;2, NtPOD1;5, NtPOD1;6, and NtPOD1;9, and NtPIN1 and NtPIN4 are upregulated in the TaZAT8-overexpressing lines. Overexpression of NtPIN1 and NtPIN4 conferred plants to enlarged RSA and elevated biomass under the Pi-starvation stress conditions. Our investigation provides insights into plant adaptation to the Pi-starvation stress mediated by distinct ZFP TFs through modulation of Pi acquisition and cellular ROS detoxicity.

An improved high-resolution satellite enthalpy flux dataset is employed to study the composites of initial (i.e., t = 0 h) latent heat flux (LHF), sensible heat flux (SHF), and their bulk variables associated with four intensity-change categories of tropical cyclones (TCs) over the western North Pacific Ocean—rapidly intensifying (RI), slowly intensifying, neutral, and weakening—in a vertical wind shear–relative coordinate system with horizontal dimensions normalized by the radius of maximum wind. Results show that RI TCs are associated with significantly higher LHF and SHF in all TC environments than non-RI TCs, which are mainly attributable to the air–sea humidity difference (DQ) and the air–sea temperature difference (DT), respectively. Higher DQ and DT are primarily due to significantly higher sea surface temperature (SST) underlying RI TCs, emphasizing the crucial role of SST in supplying more energy to TCs that undergo rapid intensification, in which LHF plays a more important role than SHF. Relative to non-RI TCs, LHF and SHF for RI TCs show a more symmetric pattern. The magnitude and pattern of air–sea enthalpy flux could serve as potential predictors for rapid intensification of TCs.

Tropical cyclone (TC) intensity prediction, especially in the warning time frame of 24–48 h and for the prediction of rapid intensification (RI), remains a major operational challenge. Sea surface temperature (SST) based empirical or theoretical maximum potential intensity (MPI) is the most important predictor in statistical intensity prediction schemes and rules derived by data mining techniques. Since the underlying SSTs during TCs usually cannot be observed well by satellites because of rain contamination and cannot be produced on a timely basis for operational statistical prediction, an ocean coupling potential intensity index (OC_PI), which is calculated based on pre-TC averaged ocean temperatures from the surface down to 100 m, is demonstrated to be important in building the decision tree for the classification of 24-h TC intensity change ΔV24, that is, RI (ΔV24 ≥ 25 kt, where 1 kt = 0.51 m s−1) and non-RI (ΔV24 < 25 kt). Cross validations using 2000–10 data and independent verification using 2011 data are performed. The decision tree with the OC_PI shows a cross-validation accuracy of 83.5% and an independent verification accuracy of 89.6%, which outperforms the decision tree excluding the OC_PI with corresponding accuracies of 83.2% and 83.9%. Specifically for RI classification in independent verification, the former decision tree shows a much higher probability of detection and a lower false alarm ratio than the latter example. This study is of great significance for operational TC RI prediction as pre-TC OC_PI can skillfully reduce the overestimation of storm potential intensity by traditional SST-based MPI, especially for the non-RI TCs.

microRNAs (miRNAs) are involved in regulating various plant developmental processes and mediating plant-adaptive responses to nutrient deprivation. In this study, the characterization of a wheat miRNA member TaMIR444a and the role of this miRNA in mediating plant tolerance to the N-starvation stress were investigated. Results indicated that the expression levels of TaMIR444a and NtMIR444a, the homologue of TaMIR444a in tobacco, were upregulated in roots and leaves under N deprivation, whereas the transcription of their target genes showed reverse expression patterns in above tissues. These results suggest that miR444a is conserved across plant species of dicots and monocots and can possibly establish the miRNA/target modules for mediating plant response to N deficiency. Overexpression of TaMIR444a in tobacco improved the plant growth feature, biomass, N content, photosynthetic parameters, and antioxidant enzymatic activities under N deprivation. Based on microarray analyses, a large number of genes were identified to be differentially expressed in the TaMIR444a-overexpressing plants; these differential genes are categorized into functional groups of signal perception and transduction, transcription regulation, primary and secondary metabolism, phytohormone response, cellular protection and defensive responsiveness, etc. qPCR analyses revealed that the nitrate transporter (NRT) genes NtNRT1.1-s, NtNET1.1-t, and NtNRT2.1 and the antioxidant enzyme genes (AEEs) NtCAT1;1, NtPOD1;3, and NtPOD4 were significantly upregulated by TaMIR444a, suggesting that the altered transcription of these NRT and AEE genes is associated with the improvement of the N acquisition and the cellular ROS detoxification in the N-deprived transgenic plants. Together, our findings demonstrate that miR444a acts as one critical regulator in mediating plant tolerance to the N-starvation stress through modulation of the regulatory networks associated with N acquisition, cellular ROS homeostasis, and carbon assimilation. Our findings have provided insights into the mechanisms of plant tolerance to N deficiency mediated by the distinct miRNA pathways.

Application of biochar to soils has been proposed as a novel approach to managing wood residuals, enhancing soil carbon (C) storage and improving soil fertility; however, the majority of biochar studies have been conducted in agricultural systems that rely on tillage and nutrient inputs associated with annual cropping schemes. Few studies have evaluated the influence of biochar on soil processes in semi-natural rangeland ecosystems that feature more complex plant communities, lack deep soil disturbance, and have relatively few external nutrient inputs. In August 2018, biochar produced using wood waste from a lumber mill in Columbia Falls, MT, USA was applied to surface soils in replicated plots at an experimental ranch in western Montana to test the impact of biochar on soil C storage and nutrient management. A series of soil biochemical properties including total soil C and nitrogen (N), microbial N functional genes, available phosphorus (P) and the net accumulation of nutrients below surface soil layer were evaluated over a one-year period following biochar addition with or without a poultry litter based organic fertilizer. Biochar used alone slightly reduced soil NH4+, significantly increased soil nitrification potential, increased the relative abundance of the bacterial amoA gene, and increased the soil nitrate (NO3−) pool size, while having no net effect on soil inorganic N accumulation below surface soil. By contrast, biochar charged with poultry litter (termed “charged biochar”) had no effect on NH4+ availability, but had a positive effect on amoA abundance. Charged biochar significantly reduced NH4+ accumulation below 25 cm depth compared to poultry litter alone. Biochar additions led to a shift towards a more fungal dominated community and a general increase in P availability. However, biochar alone also contributed to a greater amount of soluble P collected below surface soil, an effect slightly attenuated when biochar was applied with poultry litter. Soil pH increased from 5.7 to 6.9 in response to biochar addition and was one of the dominant factors governing the observed changes in soil processes. Charged biochar helped retain soil nutrients and promoted soil C storage in this semi-natural rangeland system over one growing season. Changes in these soil pools and fluxes may influence various trophic groups affecting ecosystem functioning over time.

The current study evaluated the influence of a multi-carbohydrase and phytase complex (MCPC) on the ileal and total tract digestibility of nutrients in growing pigs. A total of eight barrows (initial BW = 30.7 &plusmn, 1.1 kg) were surgically fitted with a T-cannula at the distal ileum and randomly allotted to four groups. The experiment was conducted according to a 4 &times, 4 Latin square design, each period lasting 10 days. Pigs were fed four experimental diets, which consisted of two basal diets (BD1, low phytate, BD2, high phytate) with or without MCPC containing at least 1800 U xylanase, 6600 U &alpha, arabinofuranosidase, 1244 U &beta, glucanase, and 1000 U phytase per/kg corn&ndash, soybean meal with 15% corn distillers based diet. The high phytate diet reduced (p &lt, 0.05) the apparent ileal digestibility (AID) of crude protein by 1.4% and the apparent total tract digestibility (ATTD) of organic matter, crude protein, and gross energy by 1.7, 2.3, and 1.9%, respectively, and tended to decrease (p = 0.10) the ATTD of Ca by 17.3%, relative to the low phytate diet. The dietary supplementation of the MCPC increased (p &lt, 0.05) the AID of phosphorus (P) and calcium (Ca) by 34.2% and 31.1% for BD1 and 26.7% and 41.3% for BD2, respectively, and increased (p &lt, 0.05) ATTD of crude fat, P, and Ca by 1.4%, 45.6%, and 9.6% for BD1 and 3.1%, 66.0%, and 52.7% for BD2, respectively. The MCPC supplementation did not significantly increase the AID and (or) ATTD of crude protein, organic matter, and starch. In conclusion, the dietary supplementation of the MCPC could improve the AID of P and Ca and the ATTD of crude fat, P, and Ca.

We propose a precise diamond micromachining method based on ultraviolet femtosecond laser direct writing and a mixed acid heating chemical treatment. The chemical composition of the attached clusters generated during laser ablation and their effects on morphologies were investigated in experiments. The averaged roughness of pristine and processed regions reduced to 0.64 nm and 9.4 nm from 20.5 nm and 37.4 nm, respectively. With this method, spiral zone plates (SZPs) were inscribed on a high-pressure high-temperature diamond surface as micro-optical vortex generators. The optical performances of the diamond SZPs were characterized in both experiments and simulations, which were very consistent with each other. This chemical auxiliary processing method will contribute greatly to the wide application of integration and miniaturization of diamond surface optical components.

Multilayered aluminum composites (ARB6-2N/4 N) with alternating layers of commercially purity aluminum (2N–Al) and high-purity aluminum (4N–Al) were fabricated by six cycles of accumulative roll bonding (ARB) processing. The ARB processed specimens were annealed at different temperatures. It was found that heterogeneous lamellar microstructures composed of fine-grained 2 N (hard) layers and coarse-grained 4 N (soft) layers formed in the specimens. The specimen with the heterogeneous layered structure showed a large tensile elongation with a strength following the rule of mixture. Digital image correlation analyses revealed that the deformation bands with high local strains formed in the 4 N layers but were terminated at the interfaces between the 2 N and the 4 N layers in the multilayered aluminum composites, accommodating the deformation between the hard (2 N) layers and the soft (4 N) layers during tensile deformation. Termination of the strain localization delayed macroscopic necking of the materials, leading to a better combination of high strength and good ductility in the multilayered aluminum composites.

ObjectiveTo develop and validate a set of risk scores for the prediction of gestational diabetes mellitus (GDM) before the 15th gestational week using an established population-based prospective cohort.MethodsFrom October 2010 to August 2012, 19 331 eligible pregnant women were registered in the three-tiered antenatal care network in Tianjin, China, to receive their antenatal care and a two-step GDM screening. The whole dataset was randomly divided into a training dataset (for development of the risk score) and a test dataset (for validation of performance of the risk score). Logistic regression was performed to obtain coefficients of selected predictors for GDM in the training dataset. Calibration was estimated using Hosmer-Lemeshow test, while discrimination was checked using area under the receiver operating characteristic curve (AUC) in the test dataset.ResultsIn the training dataset (total=12 887, GDM=979 or 7.6%), two risk scores were developed, one only including predictors collected at the first antenatal care visit for early prediction of GDM, like maternal age, body mass index, height, family history of diabetes, systolic blood pressure, and alanine aminotransferase; and the other also including predictors collected during pregnancy, that is, at the time of GDM screening, like physical activity, sitting time at home, passive smoking, and weight gain, for maximum performance. In the test dataset (total=6444, GDM=506 or 7.9%), the calibrations of both risk scores were acceptable (both p for Hosmer-Lemeshow test >0.25). The AUCs of the first and second risk scores were 0.710 (95% CI: 0.680 to 0.741) and 0.712 (95% CI: 0.682 to 0.743), respectively (p for difference: 0.9273).ConclusionBoth developed risk scores had adequate performance for the prediction of GDM in Chinese pregnant women in Tianjin, China. Further validations are needed to evaluate their performance in other populations and using different methods to identify GDM cases.

In this work, lignin-based nanofibers were produced from corn stalk lignin (CSL)/lignin-derived bio-oil (LBO) and polyacrylonitrile (PAN) co-polymers via electrospinning. During depolymerization, the addition of phosphotungstic acid (PTA) promoted the CSL catalytic conversion to some extent, the decrease of β-O-4 aryl ether bond, molecular weight (Mw) and polydispersity (PDI) was obvious. Compared with CSL, LBO can promotes the formation of uniform and smooth lignin-based nanofibers under high replacement rates condition and maintaining good morphology. Subsequent reactions between LBO and PAN fragments formed a better-integrated co-polymers structure containing linear PAN backbone. The Td of LBO-g-PAN was improved to 153 °C and the Tg was decreased to 96.5 °C. Rheological conclusions displayed that the viscosity of PAN/LBO/DMF was 0.082 Pa s, illustrating the polymer substance could be continuously spun into filament without causing degradation of the polymer substance. LBO-g-PAN also had high thermal stability, yielding of residual mass was 56.32 wt% at 700 °C.

A 400 nm femtosecond laser was used to ablate the surface of a high-pressure and high-temperature diamond, and subwavelength surface micro structures with a period of 100 nm were achieved. A variety of micro-nano composite surface structures were prepared by changing the polarization direction and laser scanning direction. By dynamically adjusting the laser polarization and the laser scanning tracks, a maskless direct writing fabrication of micro-nano complex structures was realized. The micro-nano patterning on an ultra-hard and super-stabile diamond provides a new idea for the preparation of friction reducing surfaces, nano imprint transfer templates, surface enhanced Raman scattering test substrates, and micro-nano optical structures.

Inverse synthetic aperture ladar(ISAL)operates at light wavelength and utilizes the synthetic aperture technique, which can obtain very high resolution two-dimensional or three dimensional image of a moving target and thus have many important applications for target detection and recognition. With a very short wavelength, 10000 times smaller compared with that of microwave radar, ISAL can get a high angle resolution with a small telescope in microseconds. However, ISAL is more sensitive to many errors, such as ladar vibration, atmospheric turbulence, moving target jitter, etc., which bring many difficulties to implement an ISAL equipment. In this article, we present an ISAL demonstration and some outdoor experimental results. The ISAL transmits wideband coherent laser which is created by modulating a phase coded signal onto a continuous laser waveform at 1550nm, and detects weak echoes reflected from moving targets by a coherent detection system. A modified phased gradient autofocus algorithm(MPGA)is utilized to suppress errors and improve image quality. The ISAL demonstration acquires high-resolution images of 3-centimeter with a distance about 200m.

Biochar is a carbon (C) rich product of thermochemical conversion of organic material that is used as a soil amendment due to its resistance to decomposition and its influence on nutrient dynamics; however, individual studies on biochar effects on phosphorus (P) and nitrogen (N) have proven inconsistent. Herein, we performed a meta-analysis of 124 published studies to evaluate the influence of biochar on available P, microbial biomass P (MBP), and inorganic N (NO3−-N and NH4+-N) in global agricultural ecosystems. Overall, the results showed that biochar applications significantly increased surface soil available P by 45% and MBP by 48% across the full range of biochar characteristics, soil type, or experimental conditions. By contrast, biochar addition to soil reduced NO3−-N concentrations by 12% and NH4+-N by 11%, but in most cases biochar added in combination with organic fertilizer significantly increased soil NH4+-N compared to controls. Biochar C:N ratio and biochar source (feedstock) strongly influenced soil P availability response to biochar where inorganic N was most influenced by biochar C:N ratio and soil pH. Biochar made from manure or other low C:N ratio materials, generated at low temperatures, or applied at high rates were generally more effective at enhancing soil available P. It is important, however, to note that most negative results were observed in short-term ( 12 months) tended to result in neutral to modest positive effects on both P and N. This meta-analysis indicates that biochar generally enhances soil P availability when added to soils alone or in combination with fertilizer. These findings provide a scientific basis for developing more rational strategies toward widespread adoption of biochar as a soil amendment for agricultural P and N management.

The clinical application of doxorubicin (DOX) in cancer chemotherapy is limited by its life-threatening cardiotoxic effects. Chrysophanol (CHR), an anthraquinone compound isolated from the rhizome of Rheum palmatum L., is considered to play a broad role in a variety of biological processes. However, the effects of CHR׳s cardioprotection in DOX-induced cardiomyopathy is poorly understood. In this study, we found that the cardiac apoptosis, mitochondrial injury and cellular PARylation levels were significantly increased in H9C2 cells treated by Dox, while these effects were suppressed by CHR. Similar results were observed when PARP1 activity was suppressed by its inhibitors 3-aminobenzamide (3AB) and ABT888. Ectopic expression of PARP1 effectively blocked this CHR׳s cardioprotection against DOX-induced cardiomyocyte injury in H9C2 cells. Furthermore, pre-administration with both CHR and 3AB relieved DOX-induced cardiac apoptosis, mitochondrial impairment and heart dysfunction in Sprague–Dawley rat model. These results revealed that CHR protects against DOX-induced cardiotoxicity by suppressing cellular PARylation and provided critical evidence that PARylation may be a novel target for DOX-induced cardiomyopathy., Graphical abstract CHR protected against doxorubicin (DOX)-induced cardiomyocytes apoptosis, mitochondrial injury and heart dysfunction by suppressing cellular PARylation in vitro and in vivo.fx1, Highlights • CHR protected against DOX-induced apoptosis, mitochondrial injury and heart dysfunction in vivo and in vitro. • PARP1 inhibition significantly suppressed DOX-induced cardiomyocyte injury. • Ectopic expression of PARP1 blocked the CHR’s cardioprotection.

Background An increasing number of breast cancer patients in Asian countries has been found to consume dietary supplements including phytoestrogen-rich Chinese herbal medicines with an expectation to alleviate the side effects of conventional cancer therapies. Purpose The question of whether estrogenic Chinese herbal medicines are beneficial or detrimental to the health of breast cancer patients remains uncertain. Study Design The present study aimed at establishing a systematic approach to look at the safety profiles of estrogenic Chinese herbal medicines (CHM). Methods The effects of estrogenic CHM on the growth of human breast cancer cells as well as the progression of breast tumors in mice have been investigated. Results Our results demonstrated that among 10 selected estrogenic CHM, the aqueous extracts of Cistanche deserticola (CD) and Dioscorea opposita (DO) at 0.4 to 1.6 mg/ml significantly stimulated cell viability in both estrogen receptor (ER)-positive (MDA-MB-361 and MCF-7) and ER-negative (SKBR3 and MDA-MB-231) breast cancer cells. However, results from animal studies showed that no significant difference was found on the size of mouse 4T1 breast tumors in CD- and DO-treated mice when compared with the control group, while the number of proliferative cells were found to be increased in DO-treated group. Besides, CD and DO treatments induced significant immunomodulatory effects on 4T1 tumor-bearing mice by increasing the production of cytokines IL-2 and IFN-γ and modulation of regulatory T-cells. Furthermore, CD and DO treatments did not stimulate, but in fact suppressed human triple-negative MDA-MB-231 breast xenografts growth in immunodeficiency mice. Conclusion The considerable concerns on the use of CD and DO in breast cancer patients could be relieved to some extents upon the findings of this pre-clinical study. The potential harmful effects of estrogenic Chinese herbal medicines on breast cancer growth should be verified in both cell-based and tumor-bearing mice models.

This study focused on the structural differences of lignin after pyridine&ndash, acetic acid&ndash, water (PAW) and dioxane&ndash, acidic water (DAW) purification processes. These structural differences included the S/G ratio, condensed structure, weight-average (MW) molecular weights, &beta, O-4 linkages and sugar content. The chemical structure of the isolated crude lignin (CL), PAW purified lignin (PPL) and DAW purified lignin (DPL) was elucidated using quantitative 13C NMR, 2D-HSQC NMR spectra, thermogravimetric analysis (TGA), gel permeation chromatography (GPC) and Fourier transform infrared spectroscopy (FTIR). The results showed that the PPL fractions contain fewer condensed structures, higher S/G ratios, more &beta, O-4 linkages, higher average MW and lower thermal degradation properties compared to the CL and DPL fractions. Furthermore, the PAW process was more selective in removing condensed units and enriching S-type lignin from CL compared to the DAW process. These results provide valuable information for understanding which purification process is more suitable to be applied for lignin.

With the stable development of new countryside construction and new-type urbanization, the changing of the landscape pattern in countryside attracts more attention, especially in the ethnic townships which are hardly accessible. To explore the development of these areas, it is crucial to understand the spatial and temporal variation of the landscape pattern. In this paper, the landscape pattern change was analyzed at both patch type level and landscape level based on the landscape ecology theory. The land use data (format: vector) got from Aerla Town (a typical Daur ethnic township in Inner Mongolia) for the duration from 2008 to 2013 was studied by ArcGIS platform and Fragstats. For the type level, the grassland, farmland, and forest turned into building land gra-dually. Regarding the landscape level, the landscape diversity index and landscape connectivity index were relatively low, the heterogeneity index and the landscape fragmentation were relatively high. With considering the correlation analysis and grey correlation of the above indexes as well as the social and economic development in Aerla Town, the results indicated that population change and GDP growth were the main driving forces of landscape pattern change. Finally, the driving forces which resulted in the variation of landscape pattern with the incorporation of the economic, cultural, policy, and natural effects were discussed. The research could provide basic information and theoretical foundation for the development of minority areas in Northeast China.随着国家新农村建设和新型城市化的稳步推进,乡村景观格局的变化开始受到广泛关注,特别是相对闭塞的少数民族乡镇,理解其景观格局的时空变化对于探索民族乡镇发展至关重要.本研究基于景观生态学理论,利用典型达斡尔族少数民族乡镇——阿尔拉镇2008—2013年间的土地利用矢量数据,通过ArcGIS和 Fragstats软件计算景观格局指数,从类型水平和景观水平上对景观格局变化进行分析.结果表明: 在类型水平上,研究区农、林、草地逐渐向建筑用地转移;在景观水平上,景观多样性指数偏低,异质性较高,连通性不强,景观破碎化程度较高.将景观指数与社会经济指标进行典型性相关分析和灰色关联分析,发现人口的变化和GDP的增长是研究区景观格局变化的最主要驱动因子.最后,结合经济、文化、政策、自然等因素探讨了导致阿尔拉镇景观格局演变的主要驱动因素,并给出相应对策,该结果可为我国东北少数民族聚居区的发展提供基础信息和理论依据.

Homocysteine (Hcy) can induce atherosclerosis through the inflammatory response and DNA methylation disorder. Our recent study has reported a novel epigenetic modified gene related to atherosclerosis -SMAD7. To further understand the pathogenesis of atherosclerosis, the current study was designed to investigate an inflammatory role of Hcy in human umbilical vein smooth muscle cells (HUVSMCs) through interfering with SMAD7 methylation. Using MALDI-TOF MS, we found that Hcy increased DNA methylation levels of SMAD7 promoter in a dose and time-dependent manner in HUVSMCs. Meanwhile, both SMAD7 mRNA and protein levels were decreased along with the increase of Hcy concentrations and treating time. Decreased SMAD7 levels led to up regulation of pro-inflammatory cytokines (TNF-α and IL-1β) expression in HUVSMCs. Furthermore, we found that activation of NF-κB pathway was the mechanism by which reduced Smad7 levels enhanced vascular inflammation. Thus, Hcy is able to activate NF-κB-mediated vascular inflammatory response via inducing hypermethylation of SMAD7 promoter in HUVSMCs. The in vitro findings supplement our recent clinical study that SMAD7 methylation as a novel marker in atherosclerosis and further elucidate the role of Hcy in atherogenesis.

Engineering material structures at the atomic level is a promising way to tune the physicochemical properties of materials and optimize their performance in various potential applications. Here, we show that the lithiation-induced amorphization of layered crystalline Pd3P2S8 activates this otherwise electrochemically inert material as a highly efficient hydrogen evolution catalyst. Electrochemical lithiation of the layered Pd3P2S8 crystal results in the formation of amorphous lithium-incorporated palladium phosphosulfide nanodots with abundant vacancies. The structure change during the lithiation-induced amorphization process is investigated in detail. The amorphous lithium-incorporated palladium phosphosulfide nanodots exhibit excellent electrocatalytic activity towards the hydrogen evolution reaction with an onset potential of −52 mV, a Tafel slope of 29 mV dec−1 and outstanding long-term stability. Experimental and theoretical investigations reveal that the tuning of morphology and structure of Pd3P2S8 (for example, dimension decrease, crystallinity loss, vacancy formation and lithium incorporation) contribute to the activation of its intrinsically inert electrocatalytic property. This work provides a unique way for structure tuning of a material to effectively manipulate its catalytic properties and functionalities. The structural modification of inactive materials to effectively engineer active catalysts is very attractive. Here, layered crystalline Pd3P2S8 is transformed by electrochemical lithiation into amorphous Li-incorporated nanodots. This process turns the inert parent material into a highly active and stable hydrogen-evolving catalyst.

Background: Angelica sinensis (AS) has long been regarded to stimulate breast cancer growth, hence the use of AS in breast cancer patients remains a major concern for both patients and practitioners. Since safety studies of herbs would be unethical to carry out in patients, the present study aimed to investigate the potential unsafe effects of AS in a systematic pre-clinical approach. Methods: Human breast cancer cells, breast orthotopic tumor-bearing mouse models as well as primary breast cancer cells from patients' tumors were used to evaluate the effect of AS hot water extract on the progression of breast tumors and/or growth of breast cancer cells. Results: We showed that AS is not that stimulatory in breast cancer both in vitro and in vivo, though AS should still be used with caution in estrogen receptor-positive breast cancer patients. This novel approach of applying breast cancer cell lines, xenograft and syngeneic tumors models, as well as primary breast cancer cells from patients' tumors in Chinese medicines safety evaluation was proven feasible. Conclusions: Our finding is important information for patients, Chinese medicine practitioners and clinicians on the safety use of AS in breast cancer, which will affect future clinical practice. Funding Statement: This study was financially supported by Food and Health Bureau HKSAR, Health and Medical Research Fund no. 12130471. Declaration of Interests: The authors declare that there is no conflict of interest. Ethics Approval Statement: The study protocol was approved by the Joint Chinese University of Hong Kong–New Territories East Cluster Clinical Research Ethics Committee (CREC Ref. No.: 2014.336) and Union Hospital Ethics Committee (EC Ref. No. EC013).

In this study, 14 wheat cultivars with contrasting yield and N use efficiency (NUE) were used to investigate the agronomic and NUE-related traits, and the N assimilation-associated enzyme activities under low and high N conditions. Under deficient-N, the cultivars with high N uptake efficiency (UpE) and high N utilization efficiency (UtE) exhibited higher plant biomass, yields, and N contents than those with medium and low NUEs. The high UpE cultivars accumulated more N than other NUE type cultivars. Under sufficient-N, the tested cultivars showed similar patterns in biomass, yield, and N content to those under deficient-N, but the varietal variations in above traits were smaller. In addition, the high UpE cultivars displayed much more of root biomass and larger of root length, surface area, and volume than other NUE type cultivars, indicating that the root morphological traits under N deprivation are closely associated with the plant biomass through its improvement of the N acquisition. The high UtE cultivars showed higher activities of nitrate reductase (NR), nitrite reductase (NIR), and glutamine synthetase (GS) at stages of seediling, heading and filling than other NUE type cultivars under both low and high N conditions. Moreover, the high UpE and UtE cultivars also displayed higher photosynthetic rate under deficient-N than the medium and low NUE cultivars. Together, our results indicated that the tested wheat cultivars possess dramatically genetic variations in biomass, yield, and NUE. The root morphological traits and the N assimilation enzymatic acitivities play critical roles in regulating N accumulation and internal N translocation under the N-starvation stress, respectively. They can be used as morphological and biochemical references for evaluation of UpE and UtE in wheat.

The standard assumption in macroeconomics that government spending is unproductive can have substantive implications for tax and spending policy. Productive government spending introduces a positive feedback between the tax rate, the productive capacity of the economy, and tax revenue. We allow marginal tax revenue to be optimally allocated between productive subsidies to human capital and utility-enhancing government consumption and calculate Laffer Curves for the US. Productive government spending yields higher revenue-maximizing tax rates, steeper slopes at low tax rates and higher peaks. The differences are particularly pronounced for the labor-tax Laffer curve. The use of tax revenue is an important determinant of the actual revenue that a tax rate increase generates.

In previous studies, we demonstrated that the green tea Camellia sinensis (CS) water extract had potent antitumor and antimetastatic effects on 4T1 breast cancer. The metronomic regimen (0.0125 mg/kg twice a week for 4 weeks) of zoledronate (ZOL) was found to be effective in decreasing tumor burden and metastasis as compared with conventional regimen. The aim of the present study was to investigate the antitumor, antimetastatic and anti-osteolytic effects of the combined use of CS water extract and metronomic ZOL against 4T1 breast carcinoma in vitro and in vivo. The results demonstrated that the combination of CS+ZOL exerted a more potent effect on lung and liver by decreasing tumor burden and metastasis, when compared to CS or metronomic ZOL as monotherapies. The combination of CS+ZOL demonstrated optimal bone protection against breast cancer-induced osteolysis for the three groups of CS, ZOL and CS+ZOL. The in vitro results further demonstrated that ZOL enhanced CS-induced apoptosis in 4T1 cells as assessed by the Annexin V-FITC/PI staining and caspase-3 activity assays. In addition, the combined use of CS+ZOL significantly inhibited 4T1 cell migration. Mechanistic studies showed that the enzyme levels of matrix metalloproteinases (MMP)-2 and MMP-9 were suppressed significantly by CS+ZOL. In conclusion, to the best of our knowledge, this is the first study to investigate the novel combined application of herbal extract CS and chemotherapy ZOL in 4T1 breast cancer. The combination of CS plus metronomic ZOL demonstrated significant antitumor, antimetastatic and anti-osteolytic effects against breast cancer, and suggested potential clinical application for breast cancer patients.

Background and Purpose The orphan nuclear receptor NOR1 belongs to the NR4A subfamily of the nuclear hormone receptor superfamily, and is involved in glucose and fat metabolism. However, its potential contribution to cardiovascular diseases remains to be assessed. Here, the roles of NOR1 in cardiac hypertrophy induced by isoprenaline and the underlying molecular mechanisms were investigated. Experimental Approach NOR1 was expressed in cardiomyocytes treated with isoprenaline. After NOR1 overexpression or knockdown in neonatal rat cardiomyocytes, cellular hypertrophy was monitored by measuring cell surface area and the mRNA of hypertrophic biomarkers. Interactions between NOR1 and PARP-1 were investigated by co-immunoprecipitation. NOR1 expression and PARP-1 activity were measured in rats with cardiac hypertrophy induced by isoprenaline. Key Results Treatment with isoprenaline significantly up-regulated NOR1 expression and PARP-1 activity both in vivo and in vitro. Specific gene silencing of NOR1 attenuated isoprenaline-induced cardiomyocyte hypertrophy, whereas NOR1 overexpression exacerbated cardiac hypertrophy. We identified a physical interaction between NOR1 and PARP-1, which was enhanced by NOR1 transfection and thereby led to PARP-1 activation. Overexpression of NOR1, but not C293Y, a NOR1 mutant lacking the PARP-1 binding activity, increased cellular surface area and the mRNA levels of atrial natriuretic factor and brain natriuretic polypeptide, effects blocked by the PARP-1 inhibitor 3-aminobenzamide or siRNA for PARP-1. Conclusions and Implications This is the first evidence that NOR1 was involved in isoprenaline-induced cardiac hypertrophy. The pro-hypertrophic effect of NOR1 can be partly attributed to its regulation of PARP-1 enzymic activity.

Background and Purpose Cryptotanshinone (CTS) is a major bioactive diterpenoid isolated from Danshen, an eminent medicinal herb that is used to treat cardiovascular disorders in Asian medicine. However, it is not known whether CTS can prevent experimental atherosclerosis. The present study was designed to investigate the protective effects of CTS on atherosclerosis and its molecular mechanisms of action. Experimental Approach Apolipoprotein E-deficient (ApoE−/−) mice, fed an atherogenic diet, were dosed daily with CTS (15, 45 mg kg−1 day−1) by oral gavage. In vitro studies were carried out in oxidized LDL (oxLDL)-stimulated HUVECs treated with or without CTS. Key Results CTS significantly attenuated atherosclerotic plaque formation and enhanced plaque stability in ApoE−/− mice by inhibiting the expression of lectin-like oxLDL receptor-1 (LOX-1) and MMP-9, as well as inhibiting reactive oxygen species (ROS) generation and NF-κB activation. CTS treatment significantly decreased the levels of serum pro-inflammatory mediators without altering the serum lipid profile. In vitro, CTS decreased oxLDL-induced LOX-1 mRNA and protein expression and, thereby, inhibited LOX-1-mediated adhesion of monocytes to HUVECs, by reducing the expression of adhesion molecules (intracellular adhesion molecule 1 and vascular cellular adhesion molecule 1). Furthermore, CTS inhibited NADPH oxidase subunit 4 (NOX4)-mediated ROS generation and consequent activation of NF-κB in HUVECs. Conclusions and Implications CTS was shown to have anti-atherosclerotic activity, which was mediated through inhibition of the LOX-1-mediated signalling pathway. This suggests that CTS is a vasculoprotective drug that has potential therapeutic value for the clinical treatment of atherosclerotic cardiovascular diseases.

This study investigated the change in microstructure and mechanical properties by tempering a low carbon dual phase steel composed of ferrite and martensite. The microstructure analysis revealed that tempering process resulted in the carbide precipitation and coarsening of martensite structures. The nanohardness of each phase (martensite and ferrite) decreased with increasing the tempering temperature. The ultimate tensile strength had a linear relationship with nanohardness ratio of martensite and ferrite. On the other hand, the uniform elongation firstly did not change by tempering at the temperature below 400 °C, but then decreased by tempering at the temperature above 400 °C with decreasing the nanohardness ratio. It was concluded that the nanohardness ratio can be a good parameter for controlling the mechanical properties of dual phase steels.

Runs distribution test is an important statistical test based on Golomb's randomness postulates. It is an important randomness test included in some famous evaluation standards and statistical test suites such as Application Notes and Interpretation of the Scheme (AIS20), the Federal Information Processing Standards (FIPS140), CryptX, and so on. However, when it is applied on some well-known good Deterministic Random Bit Generators (DRBGs), the test results show apparent bias from randomness. This shows that there exist some inaccuracies in the runs distribution test. The problem is solved and the runs distribution test through two ways is corrected. First, the degree of freedom of statistical value V is adjusted. Secondly, the expected number of different lengths runs is modified. The experiment results show that the corrected runs distribution test is more accurate under two-level evaluation approach proposed by National Institute of Standards and Technology (NIST) Special Publication (SP) 800–22.

Cardiac hypertrophy often refers to the abnormal growth of heart muscle through a variety of factors. The mechanisms of cardiomyocyte hypertrophy have been extensively investigated using neonatal rat cardiomyocytes treated with phenylephrine. α-Enolase is a glycolytic enzyme with “multifunctional jobs” beyond its catalytic activity. Its possible contribution to cardiac dysfunction remains to be determined. The present study aimed to investigate the change of α-enolase during cardiac hypertrophy and explore its role in this pathological process. We revealed that mRNA and protein levels of α-enolase were significantly upregulated in hypertrophic rat heart induced by abdominal aortic constriction and in phenylephrine-treated neonatal rat cardiomyocytes. Furthermore, knockdown of α-enolase by RNA interference in cardiomyocytes mimicked the hypertrophic responses and aggravated phenylephrine-induced hypertrophy without reducing the total glycolytic activity of enolase. In addition, knockdown of α-enolase led to an increase of GATA4 expression in the normal and phenylephrine-treated cardiomyocytes. Our results suggest that the elevation of α-enolase during cardiac hypertrophy is compensatory. It exerts a catalytic independent role in protecting cardiomyocytes against pathological hypertrophy.

In side channel attack (SCA) studies, it is widely believed that unprotected implementations leak information about the intermediate states of the internal cryptographic process. However, directly recovering the intermediate states is not common practice in today’s SCA study. Instead, most SCAs exploit the leakages in a “guess-and-determine” way, where they take a partial key guess, compute the corresponding intermediate states, then try to identify which one fits the observed leakages better. In this paper, we ask whether it is possible to take the other way around—directly learning the intermediate states from the side channel leakages. Under certain circumstances, we find that the intermediate states can be efficiently recovered with the well-studied Independent Component Analysis (ICA). Specifically, we propose several methods to convert the side channel leakages into effective ICA observations. For more robust recovery, we also present a specialized ICA algorithm which exploits the specific features of circuit signals. Experiments confirm the validity of our analysis in various circumstances, where most intermediate states can be correctly recovered with only a few hundred traces. Our approach brings new possibilities to the current SCA study, including building an alternative SCA distinguisher, directly attacking the middle encryption rounds and reverse engineering with fewer restrictions. Considering its potential in more advanced applications, we believe our ICA-based SCA deserves more research attention in the future study.

SPECK is a new family of lightweight block ciphers proposed by the U.S. National Security Agency in 2013. So far, there exist several fault analysis results on this family. In this paper, we propose an improved fault analysis on SPECK under the random byte fault model, which only needs to induce faults at one intermediate round to retrieve the whole master key. In this attack, the fault propagation properties of SPECK are fully utilized, not only to determine the locations and the values of the faults, but also to eliminate incorrect candidates of the key. Moreover, compared with the previous approaches, more characteristics of the nonlinear modular addition operation are exploited, and the relations between different pairs of ciphertexts are also taken into account, which greatly enhance the efficiency of the key recovery. Finally, the experimental results confirm the correctness and the effectiveness of our proposed attack.

In this study, the yields, yield components, agronomic, and nitrogen use efficiency (NUE) traits of the eighty-four winter wheat cultivars were investigated under nitrogen (N)-sufficient and -deficient conditions. Dramatic variations were observed in the yield and agronomic traits among the cultivars. Based on absolute yields under the N treatments, the cultivars were categorized into N efficiency groups of high, close high, medium, and low, in which the high efficiency group cultivars generally exhibited improved agronomic traits under these contrasting N-supply conditions. Additionally, the wheat cultivars were classified into various N response groups, including toleration, relative toleration, sensitive, and most sensitive based on the ratios of yield under N deficiency to that under N sufficiency. Regression correlation analyses revealed that straw biomass, total biomass, grain N amount, and straw N amount were significantly correlated with yield, suggesting that these traits can be acted as indices in evaluating yield potentials in wheat.

In previous studies, we demonstrated that green tea (Camellia sinensis, CS) water extract had potent anti-tumor and anti-metastasis effects in the 4T1 mouse breast cancer xenograft model, and the metronomic regimen (0.0125 mg/kg twice a week for 4 weeks) of zoledronic acid (ZOL) was also effective in decreasing tumor burden and metastasis when compared with the conventional regimen. This study aimed to investigate the combined use of CS water extract and metronomic ZOL against tumor metastasis and bone destruction in MDA-MB-231-TXSA human breast cancer.Female nude mice were injected with MDA-MB-231-TXSA cells into the marrow space of tibia and were treated with CS water extract and/or metronomic ZOL for 4 weeks. Tumor growth and metastasis to lungs and livers were assessed by in vivo bioluminescence imaging. Abilities of migration and invasion of MDA-MB-231-TXSA cells were also evaluated in vitro.Our results demonstrated that combination of CS and ZOL had the most potent effects on tumor burden and metastasis to bone, lung and liver, while treatment with CS or ZOL alone significantly protected the bone from cancer-induced osteolysis. In vitro, the combined use of CS + ZOL significantly inhibited MDA-MB-231-TXSA cell migration and invasion. Mechanistic zymography studies showed that the enzyme activities of MMP-9 and MMP-2 were significantly suppressed by CS and CS + ZOL.The combination of CS plus metronomic ZOL demonstrated potent anti-tumor, anti-metastasis and anti-osteolysis effects against breast cancer, suggesting the potential clinical application against breast cancer patients.

We propose that solvent-vapor annealing is an efficient method for fabricating large [6,6]-phenyl C61 butyric acid methyl (PCBM) single crystals. The morphology and size of PCBM crystals are influenced by different substrates and annealing solvents. Hexagonal PCBM crystals >150 μm in size were obtained when crystallization was performed on bare SiO2 substrate under the saturated vapor of chloroform for annealing. The proposed method was also applied to a bi-component system, which led to the spontaneous formation of PCBM and dioctylbenzothienobenzothiophene single crystals.

In the present study, IF steel specimens with different grain sizes ranging from 12 to 0.45 μm were fabricated by accumulative roll-bonding process and subsequent annealing. Tensile tests revealed that by decreasing the mean grain size down to an ultrafine range smaller than approximately 1.5 μm, yielding behavior of the IF steel gradually changed from continuous yielding to discontinuous yielding. An abrupt loss in the uniform elongation occurred, when the average grain size was smaller than about 1 μm. Hall–Petch analysis on the yield stress and uniform elongation implied that the abrupt loss in the uniform elongation in the UFG grain size range corresponded to the appearance of the discontinuous yielding behavior. As it has been found in many UFG materials, discontinuous yielding is believed to be a unique mechanical behavior of UFG materials, and it has significant importance on the uniform elongation of UFG materials.

Endothelin-1 (ET-1) is a critical molecule that involved in heart failure. It has been proved that ET-1 stimulation results in cardiac hypertrophy both in vitro and in vivo, but the mechanisms underlying remain largely unknown. In this study, we reported that cyclooxygenase-2 (COX-2) might be an important mediator of hypertrophic responses to ET-1 stimulation. In the cultured rat neonatal cardiomyocytes, ET-1 significantly upregulated the expression and activity of COX-2, which was accompanied by increase in cell surface area and BNP mRNA level. In contrast, ET-1-dependent cardiomyocyte hypertrophy was abolished by COX-2 selective inhibitors, NS-398 and celecoxib, or by COX-2 RNA interference, but the inhibitory effects could be diminished by pretreatment with PGE2. Furthermore, cyclosporin A (CsA) and knockdown of nuclear factor of activated T-cells c3 (NFATc3) inhibited the expression of COX-2 induced by ET-1, and NFATc3 could also bound to the -GGAAA- sequence in the promoter region of rat COX-2 gene, indicating that calcineurin/NFATc3 signaling participated in the transcriptional regulation of COX-2 following ET-1 treatment. These findings provided further insight into the roles of ET-1 in cardiac hypertrophy and suggested a potential therapeutic strategy against cardiac hypertrophy by inhibiting COX-2.

Accumulation of foam cells in the neointima represents a key event in atherosclerosis. We previously demonstrated that Tanshinone IIA (Tan), a lipophilic bioactive compound extracted from Salvia miltiorrhiza Bunge, inhibits experimental atherogenesis, yet the detailed mechanisms are not fully understood. In this study, we sought to explore the potential effects of Tan on lipid accumulation in macrophage foam cells and the underlying molecular mechanisms. Our data indicate that Tan treatment reduced the content of macrophages, cholesterol accumulation, and the development of atherosclerotic plaque in apolipoprotein E-deficient mice. In human macrophages, Tan ameliorated oxidized low density lipoporotein (oxLDL)-elicited foam cell formation by inhibiting oxLDL uptake and promoting cholesterol efflux. Mechanistically, Tan markedly reduced the expression of scavenger receptor class A and increased the expression of ATP-binding cassette transporter A1 (ABCA1) and ABCG1 in lipid-laden macrophages via activation of the extracellular signal-regulated kinase (ERK)/nuclear factor-erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway. Tan treatment induced the phosphorylation and nuclear translocation of Nrf2 and subsequently increased the expression of HO-1, and these effects were abolished by the specific ERK inhibitors, PD98059 and U0126. Moreover, HO-1 small interfering RNA or zinc protoporphyrin (a HO-1 inhibitor) abrogated Tan-mediated suppression of lipid accumulation in macrophages. Our current findings demonstrate that a novel HO-1-dependent mechanism is involved in the regulation of cholesterol balance by Tan.

High purity iron specimens containing 11 ppm carbon and 8 ppm nitrogen with different grain sizes were fabricated by cold rolling and subsequent annealing. It was found that the specimens exhibited entirely different yielding behavior in tensile tests depending on different cooling processes after annealing. The water-cooled specimens exhibited continuous yielding while the air-cooled ones exhibited discontinuous yielding. It was found that the Hall­Petch slope, ky, significantly changed depending on the different yielding behaviors. [doi:10.2320/matertrans.MA201326]

Nanocrystalline austenite having fully annealed and equiaxed morphology, grain sizes varying from 400nm to 1.7µm, and a large fraction of high-angle grain boundaries was prepared in Fe­24Ni­0.3C (mass%) alloy by high-pressure torsion and subsequent heat treatment. The nanostructures were formed through reverse transformation of deformation-induced martensite and recovery/recrystallization of retained austenite. The austenitic grain size decreased with increasing shear strain below the strain of 50, while it increased above the shear strain of 50 due to decreased austenite-transformation starting temperature. [doi:10.2320/matertrans.M2013324]

Interstitial free (IF) steel specimens with different mean grain sizes ranging from 0.4 to 12µm were fabricated by the accumulative roll bonding (ARB) process and subsequent annealing. Tensile tests at room temperature have revealed that by decreasing the mean grain size down to an ultra-fine range, the yielding behavior gradually changes from the continuous yielding to the discontinuous yielding, accompanying a yield drop phenomenon. It has been found that the yield stress of specimens having fine grain sizes shows extra-hardening, deviated from the original Hall­Petch relation for coarse-grained specimens in accordance with the discontinuous yielding. The Hall­Petch analysis also has indicated that the loss in the uniform elongation in the ultrafine grain size range is related to the appearance of the discontinuous yielding behavior. [doi:10.2320/matertrans.MA201317]