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1,439 results on '"Scorilas, Andreas"'

256. A comprehensive nomenclature for serine proteases with homology to tissue kallikreins

Author

Lundwall, Åke, Band, Vimla, Blaber, Michael, Clements, Judith A., Courty, Yves, Diamandis, Eleftherios P., Fritz, Hans, Lilja, Hans, Malm, Johan, Maltais, Lois J., Olsson, A. Yvonne, Petraki, Constantina, Scorilas, Andreas, Sotiropoulou, Georgia, Stenman, Ulf-Håkan, Stephan, Carsten, Talieri, Maroulio, and Yousef, George M.

Published
2006

274. A 3′ tRNA‐derived fragment produced by tRNALeuAAG and tRNALeuTAG is associated with poor prognosis in B‐cell chronic lymphocytic leukemia, independently of classical prognostic factors.

Author

Katsaraki, Katerina, Adamopoulos, Panagiotis G., Papageorgiou, Sotirios G., Pappa, Vasiliki, Scorilas, Andreas, and Kontos, Christos K.

Subjects
CHRONIC lymphocytic leukemia, PROGNOSIS, CHRONIC leukemia
Abstract

Objective: 3′ tRNA‐derived fragments (3′ tRFs) are important epigenetic regulators in normal and pathological conditions. In this study, we aimed to explore the potential value of a 3′ tRF as a prognostic and/or screening biomarker for B‐cell chronic lymphocytic leukemia (B‐CLL). Methods: Publicly available next‐generation sequencing data from 20 B‐CLL cases were analyzed, followed by prediction of targets of the most abundantly and ubiquitously expressed 3′ tRFs, leading to selection of tRF‐LeuAAG/TAG. PBMCs were isolated from blood samples of 91 B‐CLL patients and 43 non‐leukemic donors, followed by total RNA extraction, in‐vitro polyadenylation, and first‐strand cDNA synthesis. Next, a real‐time quantitative PCR (qPCR) assay was developed for the accurate quantification of tRF‐LeuAAG/TAG and applied in all samples, prior to biostatistical analysis. Results: High tRF‐LeuAAG/TAG levels are associated with inferior overall survival (OS) of B‐CLL patients. The unfavorable significance of tRF‐LeuAAG/TAG was independent of established prognostic factors in B‐CLL. Stratified Kaplan‐Meier OS analysis uncovered the unfavorable prognostic role of high tRF‐LeuAAG/TAG levels for patients in Binet A or Rai I stage, negative CD38 expression, mutated, or unmutated IGHV genomic locus. Conclusion: Our approach revealed the independent prognostic value of a particular 3′ tRF, derived from tRNALeuAAG and tRNALeuTAG (tRF‐LeuAAG/TAG) in B‐CLL. [ABSTRACT FROM AUTHOR]

Published
2021
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275. L-DOPA decarboxylase mRNA expression is associated with tumor stage and size in head and neck squamous cell carcinoma: a retrospective cohort study

Author

Geomela Panagiota-Aikaterini, Kontos Christos K, Yiotakis Ioannis, Fragoulis Emmanuel G, and Scorilas Andreas

Subjects
Oral cancer, Larynx, Tongue, Tumor biomarkers, Quantitative real-time PCR, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background Head and neck squamous cell carcinoma (HNSCC) represents one of the most commonly diagnosed malignancies worldwide. The DDC gene encodes L-DOPA decarboxylase, an enzyme catalyzing the decarboxylation of L-DOPA to dopamine. We have recently shown that DDC mRNA is a significant predictor of patients’ prognosis in colorectal adenocarcinoma and prostate cancer. The aim of the current study was to analyze the DDC mRNA expression in HNSCC patients. Methods 53 malignant tumors were resected from the larynx, pharynx, tongue, buccal mucosa, parotid glands, and nasal cavity, as well as from 34 adjacent non-cancerous tissues of HNSCC patients, and were homogenized. Total RNA was isolated and converted into first-strand cDNA. An ultrasensitive real-time PCR method based on the SYBR Green chemistry was used for DDC mRNA quantification in head and neck tissue specimens. Relative quantification was performed using the comparative Ct (2-ddCt) method. Results DDC mRNA levels were lower in squamous cell carcinomas (SCCs) of the larynx and tongue than in adjacent non-cancerous tissue specimens. Furthermore, low DDC mRNA expression was noticed in laryngeal and tongue tumors of advanced TNM stage or bigger size, compared to early-stage or smaller tumors, respectively. No statistically significant differences were observed between SCCs resected from pharynx, buccal mucosa, or nasal cavity, and their normal counterparts. Conclusion This is the first study examining the DDC mRNA expression in HNSCC. According to our results, DDC mRNA expression may constitute a potential prognostic biomarker in tongue and/or larynx SCCs, which principally represent the overwhelming majority of HNSCC cases.

Published
2012
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277. Alternative Splicing Detection Tool-a novel PERL algorithm for sensitive detection of splicing events, based on next-generation sequencing data analysis

Author

Adamopoulos, Panagiotis G. Theodoropoulou, Margarita C. and Scorilas, Andreas

Abstract

Next-generation sequencing (NGS) can provide researchers with high impact information regarding alternative splice variants or transcript identifications. However, the enormous amount of data acquired from NGS platforms make the analysis of alternative splicing events hard to accomplish. For this reason, we designed the “Alternative Splicing Detection Tool” (ASDT), an algorithm that is capable of identifying alternative splicing events, including novel ones from high-throughput NGS data. ASDT is available as a PERL script at http://aias.biol.uoa.gr/similar to mtheo and can be executed on any system with PERL installed. In addition to the detection of annotated and novel alternative splicing events from high-throughput NGS data, ASDT can also analyze the intronic regions of genes, thus enabling the detection of novel cryptic exons residing in annotated introns, extensions of previously annotated exons, or even intron retentions. Consequently, ASDT demonstrates many innovative and unique features that can efficiently contribute to alternative splicing analysis of NGS data.

Published
2018

278. Quantitative analysis and study of the mRNA expression levels of apoptotic genes BCL2, BAX and BCL2L12 in the articular cartilage of an animal model of osteoarthritis

Author

Kourtis, Aristotelis Adamopoulos, Panagiotis G. Papalois, Apostolos Iliopoulos, Diinit Rios C. Babis, George C. and Scorilas, Andreas

Abstract

Background: Given that apoptosis of chondrocytes is one of the most important factors related to the pathogenesis of osteoarthritis (OA), the recent research interest adds progress not only to the knowledge of the molecular signals that mediate apoptosis but also to find new therapeutic targets. This study attempts to investigate the differential expression of BCL2 family genes in the articular cartilage of an experimental animal model of OA. Methods: In total, 26 New Zealand white rabbits underwent an anterior cruciate ligament transaction, 26 more were subjected to a placebo surgery and 18 specimens constituted the control non-operated group. Thirteen weeks later, samples of cartilage from the osteoarthritic and non-osteoarthritic knees were collected and subjected to analysis of the BCL2, BAX and BCL2L12 gene expression at the mRNA level. Results: Installed osteoarthritic alterations of varied intensity and of grade 1 up to grade 5, were confirmed according to the OARSI system. Contrary to the physiologically healthy samples, in the osteoarthritic samples the mRNA expression levels of MX and BCL2L12 genes were found significantly upregulated by signals which can activate apoptosis. However, the difference between BCL2 mRNA expression levels in healthy and osteoarthritic samples was not supported statistically. Conclusions: Since apoptosis is the main feature of the cartilage degeneration in OA, the effective inhibition of apoptosis of chondrocytes can provide novel and interesting therapeutic strategies for the treatment of OA. Therefore, BAX and BCL2L12 are highlighted as potential therapeutic targets in OA.

Published
2018

279. miR-203 is an independent molecular predictor of prognosis and treatment outcome in ovarian cancer: a multi-institutional study

Author

Panoutsopoulou, Konstantina, primary, Avgeris, Margaritis, primary, Mavridis, Konstantinos, primary, Dreyer, Tobias, primary, Dorn, Julia, primary, Obermayr, Eva, primary, Reinthaller, Alexander, primary, Michaelidou, Kleita, primary, Mahner, Sven, primary, Vergote, Ignace, primary, Vanderstichele, Adriaan, primary, Braicu, Ioana, primary, Sehouli, Jalid, primary, Zeillinger, Robert, primary, Magdolen, Viktor, primary, and Scorilas, Andreas, primary

Published
2019
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284. HPV16 E6/E7 expression in circulating tumor cells in oropharyngeal squamous cell cancers: A pilot study

Author

Economopoulou, Panagiota, primary, Koutsodontis, George, additional, Avgeris, Margaritis, additional, Strati, Areti, additional, Kroupis, Christos, additional, Pateras, Ioannis, additional, Kirodimos, Euthymios, additional, Giotakis, Evangelos, additional, Kotsantis, Ioannis, additional, Maragoudakis, Pavlos, additional, Gorgoulis, Vassilis, additional, Scorilas, Andreas, additional, Lianidou, Evi, additional, and Psyrri, Amanda, additional

Published
2019
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287. Revisiting Histone Deacetylases in Human Tumorigenesis: The Paradigm of Urothelial Bladder Cancer

Author

Giannopoulou, Aikaterini, primary, Velentzas, Athanassios, additional, Konstantakou, Eumorphia, additional, Avgeris, Margaritis, additional, Katarachia, Stamatia, additional, Papandreou, Nikos, additional, Kalavros, Nikolas, additional, Mpakou, Vassiliki, additional, Iconomidou, Vassiliki, additional, Anastasiadou, Ema, additional, Kostakis, Ioannis, additional, Papassideri, Issidora, additional, Voutsinas, Gerassimos, additional, Scorilas, Andreas, additional, and Stravopodis, Dimitrios, additional

Published
2019
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289. Gene-Specific Intron Retention Serves as Molecular Signature that Distinguishes Melanoma from Non-Melanoma Cancer Cells in Greek Patients

Author

Giannopoulou, Aikaterini, primary, Konstantakou, Eumorphia, additional, Velentzas, Athanassios, additional, Avgeris, Socratis, additional, Avgeris, Margaritis, additional, Papandreou, Nikos, additional, Zoi, Ilianna, additional, Filippa, Vicky, additional, Katarachia, Stamatia, additional, Lampidonis, Antonis, additional, Prombona, Anastasia, additional, Syntichaki, Popi, additional, Piperi, Christina, additional, Basdra, Efthimia, additional, Iconomidou, Vassiliki, additional, Papadavid, Evangelia, additional, Anastasiadou, Ema, additional, Papassideri, Issidora, additional, Papavassiliou, Athanasios, additional, Voutsinas, Gerassimos, additional, Scorilas, Andreas, additional, and Stravopodis, Dimitrios, additional

Published
2019
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290. The lysine‐specific methyltransferase KMT 2C/ MLL 3 regulates DNA repair components in cancer

Author

Rampias, Theodoros, primary, Karagiannis, Dimitris, additional, Avgeris, Margaritis, additional, Polyzos, Alexander, additional, Kokkalis, Antonis, additional, Kanaki, Zoi, additional, Kousidou, Evgenia, additional, Tzetis, Maria, additional, Kanavakis, Emmanouil, additional, Stravodimos, Konstantinos, additional, Manola, Kalliopi N, additional, Pantelias, Gabriel E, additional, Scorilas, Andreas, additional, and Klinakis, Apostolos, additional

Published
2019
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293. Down-regulation of kallikrein-related peptidase 5 (KLK5) expression in breast cancer patients: a biomarker for the differential diagnosis of breast lesions

Author

Avgeris Margaritis, Papachristopoulou Georgia, Polychronis Athanasios, and Scorilas Andreas

Subjects
KLK5, Breast Cancer, Cancer Biomarkers, Tumor Markers, KLKs, Kallikreins, Serine Proteases, Proteolysis, Medicine
Abstract

Abstract Background Kallikrein-related peptidase 5 (KLK5) is a secreted trypsin-like protease of the KLK family, encoded by the KLK5 gene. KLK5 has been found to cleave various extracellular matrix components, as well as to activate several other KLK proteases, triggering the stimulation of tissue microenvironment proteolytic cascades. Material and Methods KLK5 expression levels were quantified in 102 cancerous and benign breast tissue specimens, obtained by randomly chosen patients, using RT-qPCR assay. Subsequently, advanced biostatistics were applied in order to analyze the KLK5 expression profile in the two patients' cohorts and also to evaluate its clinical significance for the discrimination of breast tumors. Results A statistically significant (p < 0.001) down-regulation of the KLK5 expression levels were observed in the malignant specimens compared to the benign ones. Logistic regression and ROC curve analysis revealed the significant (p < 0.001) and the independent (p < 0.001) value of the KLK5 expression quantification, for the discrimination of the malignant from the benign mammary gland biopsies. Moreover, KLK5 expression levels correlate with the pre-menopausal status (p < 0.005) as well as the ER-negative staining (p = 0.028) of women with breast cancer. Conclusions The quantification of KLK5 expression in breast tissue biopsies may be considered as a novel and independent biomarker for the differential diagnosis between malignant and benign tumors of the mammary gland.

Published
2011
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295. BCL2L12 improves risk stratification and prediction of BFM-chemotherapy response in childhood acute lymphoblastic leukemia

Author

Avgeris, Margaritis, primary, Stamati, Lamprini, additional, Kontos, Christos K., additional, Piatopoulou, Despina, additional, Marmarinos, Antonios, additional, Xagorari, Marieta, additional, Baka, Margarita, additional, Doganis, Dimitrios, additional, Anastasiou, Theodora, additional, Kosmidis, Helen, additional, Gourgiotis, Dimitrios, additional, and Scorilas, Andreas, additional

Published
2018
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