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INTRODUCTION The primary goal of this study was to compare gastrointestinal symptom reduction in patients on bismuth-containing quadruple eradication therapy supplemented with Lactobacillus reuteri strains (DSM 17938 and ATCC PTA 6475) or placebo. MATERIALS AND METHODS This was randomized, double-blind, parallel-arm, placebo-controlled clinical trial. Patients received first-line an eradication regimen based on bismuth subcitrate potassium, metronidazole, tetracycline hydrochloride (three-in-one capsules), and omeprazole 40 mg twice a day for ten days, plus a probiotic or placebo tablet for 30 days. During follow-up gastrointestinal symptoms were assessed using an evaluation scale (GSRS), and adverse events were collected at 0, 14, 28, and 56 days. RESULTS A total of 80 patients were included from February 2018 to May 2019 at a single site. Eradication therapy was effective for 85% of patients, with no differences between treatment arms. In the group receiving the probiotic, abdominal pain decreased in 42% of patients, compared with 19% in the control group (OR: 0.27; CI, 0.13-0.58; p < 0.001), and abdominal distension decreased in 25% versus 17% in the control group (OR: 0.24; IC, 0.19-0.84; p < 0.001); Conclusions: Treatment with L. reuteri only reduced abdominal pain and distension. Further studies are needed to establish the role of probiotics as adjuvant therapy in H. pylori eradication.

Acute myocarditis is an inflammatory disease of the myocardium, and it can present as severe heart failure in children. Differential diagnosis with genetic cardiomyopathy can be difficult. The objective of this study is to identify patterns of clinical presentation and to assess invasive and non-invasive measures to differentiate patients with acute myocarditis from patients with dilated genetic cardiomyopathy. We performed a retrospective descriptive study of all paediatric patients (0–16 years old) that presented with new-onset heart failure with left ventricle ejection fraction

Neuropilin-1 (NRP1) is a transmembrane protein involved in numerous cellular functions which has had increasing interest from cancer researchers. Liver cancer and colorectal cancer (CRC) are two of the most frequent and deadly tumors with a complex pharmacological framework. Here, we assessed the prognostic, diagnostic and clinicopathological value of NRP1 in liver cancer and CRC patients. We searched PubMed, Scopus, Web of Science, Embase and Cochrane Library databases for articles evaluating the NRP1 correlation with survival parameters, tumor development or clinicopathological features. Hazard ratios and odds ratios with 95% confidence intervals were extracted or estimated by Parmar method and pooled to evaluate the overall effect size with STATA 16 software. Heterogeneity was analyzed by chi-square-based Q test and I2 statistic, along with meta-regression and subgroup analysis, and publication bias was assessed by funnel plot asymmetry and Egger’s test. The study protocol was registered in PROSPERO (CRD42022307062). NRP1 overexpression was significantly correlated with lower survival in liver cancer patients and with tumor development in hepatocarcinoma patients, and was strongly correlated with an increased risk of vascular invasion in liver cancer and metastasis in CRC and liver tumors. These results support the role of NRP1 as a potential and useful biomarker in both types of cancer.

Yolanda Arroyo Pizarro es una reconocida escritora puertorriqueña y activista por los derechos de las comunidades LGBTI y afrodescendientes. Su obra literaria se centra en retratar las subjetividades cuir y en celebrar la afroidentidad. En este artículo vinculo las distintas arenas en las que la autora refrenda su compromiso político con las comunidades vulnerables para proponer nuevas lecturas del género autobiográfico, que tienen en cuenta plataformas de construcción del yo tan paradigmáticas como Facebook. Esta estrategia, a su vez, entra en coherencia con los propósitos de la autora fundados en la creación de redes de apoyo entre grupos excluidos y en la descolonización profunda. De manera que análisis y visibilización de distintas formas de representación de cuerpos y subjetividades se unen en un estudio que, en última instancia, cuestiona los parámetros en los que se asientan instituciones tan emparentadas como escritura, género y nación. Yolanda Arroyo Pizarro is a renowned Puerto Rican writer and activist for LGBTI rights and Afro-Caribbean communities. Her literature focuses on the portrait of queer subjecti- vities and the celebration of Afro-American identity. In this paper, I connect the different arenas in which the author corroborates her political engagement in order to propose a new perspective on autobiography as a literary genre, including such a paradigmatic platform for self-representation as Facebook. This strategy, in turn, is coherent with the author’s proposal, since it is founded on the creation of a network of support for vulnerable communities and on the defense of a deep decolonization. Accordingly, the analysis and visibility of different ways to represent bodies and subjectivities converge in a study that, ultimately, questions the parameters in which writing, genre and nation are founded and interconnected.

Background Infections are among the leading causes of maternal mortality and morbidity. The Global Maternal Sepsis and Neonatal Initiative, launched in 2016 by WHO and partners, sought to reduce the burden of maternal infections and sepsis and was the basis upon which the Global Maternal Sepsis Study (GLOSS) was implemented in 2017. In this Article, we aimed to describe the availability of facility resources and services and to analyse their association with maternal outcomes. Methods GLOSS was a facility-based, prospective, 1-week inception cohort study implemented in 713 health-care facilities in 52 countries and included 2850 hospitalised pregnant or recently pregnant women with suspected or confirmed infections. All women admitted for or in hospital with suspected or confirmed infections during pregnancy, childbirth, post partum, or post abortion at any of the participating facilities between Nov 28 and Dec 4 were eligible for inclusion. In this study, we included all GLOSS participating facilities that collected facility-level data (446 of 713 facilities). We used data obtained from individual forms completed for each enrolled woman and their newborn babies by trained researchers who checked the medical records and from facility forms completed by hospital administrators for each participating facility. We described facilities according to country income level, compliance with providing core clinical interventions and services according to women's needs and reported availability, and severity of infection-related maternal outcomes. We used a logistic multilevel mixed model for assessing the association between facility characteristics and infection-related maternal outcomes. Findings We included 446 facilities from 46 countries that enrolled 2560 women. We found a high availability of most services and resources needed for obstetric care and infection prevention. We found increased odds for severe maternal outcomes among women enrolled during the post-partum or post-abortion period from facilities located in low-income countries (adjusted odds ratio 1middot84 [95% CI 1middot05-3middot22]) and among women enrolled during pregnancy or childbirth from non-urban facilities (adjusted odds ratio 2middot44 [1middot02-5middot85]). Despite compliance being high overall, it was low with regards to measuring respiratory rate (85 [24%] of 355 facilities) and measuring pulse oximetry (184 [57%] of 325 facilities). Interpretation While health-care facilities caring for pregnant and recently pregnant women with suspected or confirmed infections have access to a wide range of resources and interventions, worse maternal outcomes are seen among recently pregnant women located in low-income countries than among those in higher-income countries; this trend is similar for pregnant women. Compliance with cost-effective clinical practices and timely care of women with particular individual characteristics can potentially improve infection-related maternal outcomes. Funding UNDP/UNFPA/UNICEF/WHO/World Bank Special Programme of Research, Development and Research Training in Human Reproduction, WHO, Merck for Mothers, and US Agency for International Development. Copyright (c) 2021 World Health Organization; licensee Elsevier. This is an Open Access article under the CC BY 3.0 IGO license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In any use of this article, there should be no suggestion that WHO endorses any specific organisation, products, or services. The use of the WHO logo is not permitted. This notice should be preserved along with the article's original URL.

Achieving awareness of a company’s corporate social responsibility (CSR) activities is a necessary requisite for CSR’s potentially positive effect on customer behavior. CSR awareness is particularly relevant since consumers and other stakeholders have generally little knowledge of most company’s CSR involvement.

We wish to thank the Associate Editor and two Anonymous Referees for their comments, which have helped to significantly improve this work. Any remaining shortcomings are the responsibility of the authors. Research funded by the Spanish Ministry of Ciencia e Innovaci?n under project ECO2017-86402-C2-1-R.

In recent decades, packaged fruit has gained market ground over loose fruit, and fruit containers have become a source of information for consumers. This study approaches three objectives related to consumer information needs for decision making when purchasing fruit: (1) Determine if consumers’ choice of packaged fruit rather than loose fruit is motivated by their interest in the information provided on packaging; (2) identify information gaps on fruit packaging labels; (3) identify those sensory attributes that consumers consider to be of major interest to be included in sensory labels of different fruit types. The study was based on an online questionnaire answered by 394 fruit consumers. Ninety percent of the participants stated having an interest in receiving information when purchasing fruit, but their choice between packaged or loose fruit was not conditioned by their information needs. Moreover, a gap between information interest and information use was detected as their final purchase decisions were not always based on the provided information. ‘Harvest date’, ‘production method’, ‘percentage of the price received by the farmer’, ‘applied treatments’, ‘sensory characteristics’, and ‘environmental information’ were identified as the major information gaps, as these labels were unavailable for a high percentage of consumers, who stated their interest in them. According to consumers, sensory labels should include information about ‘sweetness’ and ‘flavor intensity’ irrespectively of fruit type. ‘Sourness’ and ‘juiciness’ attributes were particularly interesting for citrus, as ‘sourness’ and ‘firmness’ were for kiwi. Information about texture properties was required for pome and stone fruit. Other attributes, such as easiness to peel, were important only for citrus fruit.

A new subcutaneous formulation of the infliximab biosimilar CT-P13 has recently been developed for the treatment of inflammatory bowel disease (IBD), providing response rates similar to intravenous treatment. In an effort to limit patient attendance at intravenous infusion centers and to maintain biological treatment during the COVID-19 pandemic, the use of this new formulation was requested. The objective of this observational, retrospective, and descriptive study was to assess CT-P13 efficacy and safety after switching from intravenous to subcutaneous formulation in patients with IBD receiving maintenance therapy. This article shows preliminary results after six months of follow-up.

Melatonin (N-acetyl-5-methoxytryptamine) is an indoleamine with antioxidant, chronobiotic and anti-inflammatory properties; reduced levels of this hormone are associated with higher risk of cancer. Several beneficial effects of melatonin have been described in a broad number of tumors, including liver cancers. In this work we systematically reviewed the publications of the last 15 years that assessed the underlying mechanisms of melatonin activities against liver cancers, and its role as coadjuvant in the treatment of these tumors. Literature research was performed employing PubMed, Scopus and Web of Science (WOS) databases and, after screening, 51 articles were included. Results from the selected studies denoted the useful actions of melatonin in preventing carcinogenesis and as a promising treatment option for the primary liver tumors hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), either alone or in combination with other compounds. Different processes were modulated by the indole, such as inhibition of oxidative stress, proliferation, angiogenesis and invasion, promotion of immune system response, cell cycle arrest and apoptosis, as well as recovery of circadian rhythms and autophagy modulation. Taken together, the present systematic review highlights the evidence that document the potential role of melatonin in improving the landscape of liver tumor treatment.

The acquisition of scientific competencies for the application of evidence-based practice (EBP) is considered an essential part of healthcare education programs in order to improve clinical effectiveness. An examination of scientific skills in occupational therapists may be helpful in understanding their current practice as well as being useful in providing a basis for applying suitable approaches to the development and implementation of EBP. Hence, this study was designed with a double main objective: (1) to describe the level of acquisition of scientific skills and academic achievement in Spanish-speaking occupational therapists; (2) to examine the factors associated with these skills. The screening for Scientific Skills in Occupational Therapists (HAbilidades Científicas en Terapeutas Ocupacionales), the HACTO-Screen, is an online cross-sectional survey divided into five sections: sociodemographic data, academic and professional background; assessment of scientific skills; research training and development needs; experience as a researcher and/or academic. A total sample of 1159 occupational therapists finally participated. Main associations will be analyzed using multiple linear and/or Poisson regression models with/without robust variance. Our findings will provide valuable insights on the research skills and associated factors in a large sample of Spanish-speaking occupational therapists. The results will also be helpful to enhance research training and research career development in occupational therapy in order to promote the use of EBP.

We report the case of a 67-year-old male who was referred to our hospital due to severe abdominal pain and fever. His past medical history included a total gastrectomy surgery for stomach cancer. Abdominal examination showed intense pain on deep palpation in the epigastric region. Laboratory test revealed elevated C-reactive protein (235 mg/l) and amylase (1,789 U/l). Computerized tomography reported a pancreatic collection with the presence of air bubbles inside and areas of necrotic pancreatic parenchyma.

A proportion of de novo variants in patients affected by genetic disorders, particularly those with autosomal dominant (AD) inheritance, could be the consequence of somatic mosaicism in one of the progenitors. There is growing evidence that germline and somatic mosaicism are more common and play a greater role in genetic disorders than previously acknowledged. In Marfan syndrome (MFS), caused by pathogenic variants in the fibrillin-1 gene (Employing an amplicon-based deep sequencing (ADS) method, we carried out a systematic analysis of 60 parents of 30Out of the 60 parents studied (30 families), the majority (n=51, 85%) had a systemic score of 0, seven had a score of 1 and two a score of 2, all due to minor criteria common in the normal population. We detected two families with somatic mosaicism in one of the progenitors, with a rate of 6.6% (2/30) of apparently de novo cases.The search for parental somatic mosaicism should be routinely implemented in de novo cases of MFS, to offer appropriate genetic and reproductive counselling as well as to reveal masked, isolated clinical signs of MFS in progenitors that may require specific follow-up.

STAT3 and STAT5B (STAT3/STAT5B) mutations are the most common mutations in T-cell large granular lymphocytic leukemia (T-LGLL) and chronic lymphoproliferative disorders of NK cells (CLPD-NK), but their clinical impact remains unknown. We investigated the frequency and type of STAT3/STAT5B mutations in FACS-sorted populations of expanded T/NK-LGL from 100 (82 clonal, 6 oligoclonal, 12 polyclonal) patients, and its relationship with disease features. Seventeen non-LGL T-CLPD patients and 628 age-matched healthy donors were analyzed as controls. STAT3 (n = 30) and STAT5B (n = 1) mutations were detected in 28/82 clonal T/NK-LGLL patients (34%), while absent (0/18, 0%) among oligoclonal/polyclonal LGL-lymphocytosis. Mutations were found across all diagnostic subgroups: TCD8+-LGLL, 36%, CLPD-NK, 38%, TCD4+-LGLL, 7%, T&alpha, &beta, +DP-LGLL, 100%, +DN-LGLL, 50%, T&gamma, &delta, +-LGLL, 44%. STAT3-mutated T-LGLL/CLPD-NK showed overall reduced (p &lt, 0.05) blood counts of most normal leukocyte subsets, with a higher rate (vs. nonmutated LGLL) of neutropenia (p = 0.04), severe neutropenia (p = 0.02), and cases requiring treatment (p = 0.0001), together with a shorter time-to-therapy (p = 0.0001), particularly in non-Y640F STAT3-mutated patients. These findings confirm and extend on previous observations about the high prevalence of STAT3 mutations across different subtypes of LGLL, and its association with a more marked decrease of all major blood-cell subsets and a shortened time-to-therapy.

We assessed the association between body mass index (BMI) and sensory processing in 445 Spanish children aged 3&ndash, 7 from the InProS project. Child sensory processing was measured using the short sensory profile (SSP), an atypical sensory performance was defined as an SSP total score &lt, 155 and scores of tactile sensitivity &lt, 30, taste/smell sensitivity &lt, 15, movement sensitivity &lt, 13, under-responsive/seeks sensation &lt, 27, auditory filtering &lt, 23, low energy/weak &lt, 26, and visual/auditory sensitivity &lt, 19. The BMI was calculated according to the cutoffs by the World Health Organization for children aged 0&ndash, 5 and 5&ndash, 19 years. We used multiple Poisson regression models with robust variance to obtain prevalence ratios (PR). No associations between children&rsquo, s overweight and obesity and the prevalence of atypical sensory outcomes were observed. A one-point increase in BMI was significantly associated with a higher prevalence of atypical tactile sensitivity (PR = 1.07, 95% CI: 1.02, 1.12). A statistically marginal association was also observed for atypical total SSP (PR = 1.03, 95% CI: 1.00, 1.07) and atypical movement sensitivity (PR = 1.05, 95% CI: 1.00, 1.10). To our knowledge, this is the first time the association between children&rsquo, s BMI and sensory processing has been reported. Our findings suggest that sensory processing issues may play a part in the complex context of childhood obesity. Further research is required to confirm these findings.

Highlights • First record of presence the RYR1 deleterious allele in wild pigs from Argentina. • The second work that reports it in wild pigs worldwide. • We found 6.6% of wild pigs carried the RYR1 gene mutation. • Evidence on a possible genetic flow between wild and domestic pigs. • Wild pigs could be a possible reservoir of the deleterious allele of the RYR1 gene., Porcine Stress Syndrome (PSS) is a disorder codified by the ryanodine receptor 1 gene (RYR1) and affects both animal welfare and the quality of the meat product. As a consequence, individuals with this syndrome generate great worldwide economic losses in the porcine industry. In Argentina, the Buenos Aires Province is the most involved on this activity, and productions are to be in open field with a higher frequency of pigs with diverse pathologies. On the other hand, the biggest and oldest wild pigs population is located on the Atlantic coast of Buenos Aires Province, which presents a continuous bidirectional flow of individuals with the productive areas nearby. The aim of this study is to detect the presence of the RYR1 deleterious allele in the wild population from the Atlantic coast of Buenos Aires, in order to evaluate its possible role as a genetic reservoir for said allele. For this purpose, 106 wild pigs from 28 sites were studied, finding a 6.6% of carrier individuals, indicating that the wild population is not free of this allele. This constitutes the first analysis to detect the presence of the RYR1 deleterious allele, associated to the PSS in wild pigs from Argentina, being one of the few studies to report it worldwide and suggesting wild pigs populations to be a possible genetic reservoir for this disease.

In recent years, a decreasing trend in fruit consumption has been detected in Mediterranean countries, with the consequent risk for the population’s health. The objective of this study was to obtain consumer knowledge that can be useful to promote fruit consumption by designing specific interventions. This study was conducted in Spain as its inhabitants have traditionally adhered to the Mediterranean diet. Firstly, four fresh fruit types were identified based on the consumer perception of the fruit characteristics that condition the eating process (fruit size, the need for cutlery to peel/eat fruit, and susceptibility to be spoiled during transportation). Then consumer perception of situational appropriateness of six different fruit types (the 4 types of fresh fruit previously identified, dehydrated non-traditional fruit (DF), and fresh-cut fruit ready to eat on the go (FCF)) was investigated by the Item-By-Use method using Check-All-That Apply (CATA) questions. The potential of DF and FCF to broaden fruit consumption situations, and barriers for their consumption, were evaluated. Fresh fruits, particularly ‘easy-to-peel’ ones like mandarins or bananas, were those preferred by consumers in most evaluated contexts. DF were considered mainly appropriate to be consumed ‘As an ingredient’ and ‘As a healthy snack’, while FCF were more suitable ‘To be included in school lunchboxes’ and ‘To eat immediately’. According to our results, these two processed fruit types can help to increase the fruit consumption of a non-negligible percentage of the population (38% of participants), but it is necessary to overcome the barriers related mostly to sensory properties, plastic packaging and consumer misperception of fewer healthy properties compared to fresh fruit.

Cercospora species cause cercospora leaf blight (CLB) and purple seed stain (PSS) on soybean. Because there are few resistant soybean varieties available, CLB/PSS management relies heavily upon fungicide applications. Sensitivity of 62 Argentinian Cercospora isolates to demethylation inhibitor (DMI), methyl benzimidazole carbamate (MBC), quinone outside inhibitor (QoI), succinate dehydrogenase inhibitor (SDHI) fungicides, and mancozeb was determined in this study. All isolates were sensitive to difenoconazole, epoxiconazole, prothioconazole, tebuconazole, and cyproconazole (EC50 values ranged from 0.006 to 2.4 µg/ml). In contrast, 51% of the tested isolates were sensitive (EC50 values ranged from 0.003 to 0.2 µg/ml), and 49% were highly resistant (EC50 > 100 µg/ml) to carbendazim. Interestingly, all isolates were completely resistant to azoxystrobin, trifloxystrobin, and pyraclostrobin, and insensitive to boscalid, fluxapyroxad, and pydiflumetofen (EC50 > 100 µg/ml). The G143A mutation was detected in 82% (53) of the QoI-resistant isolates and the E198A mutation in 97% (31) of the carbendazim-resistant isolates. No apparent resistance mutations were detected in the succinate dehydrogenase genes (subunits sdhB, sdhC, and sdhD). Mancozeb completely inhibited mycelial growth of the isolates evaluated at a concentration of 100 µg/ml. All Argentinian Cercospora isolates were sensitive to the DMI fungicides tested, but we report for the first time resistance to QoI and MBC fungicides. Mechanism(s) other than fungicide target-site modification may be responsible for resistance of Cercospora to QoI and MBC fungicides. Moreover, based on our results and on the recent introduction of SDHI fungicides on soybean in Argentina, Cercospora species causing CLB/PSS are insensitive (naturally resistant) to SDHI fungicides. Insensitivity must be confirmed under field conditions. Fil: Sautua, Francisco J.. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina Fil: Doyle, Vinson P.. No especifíca; Fil: Price, Paul P.. No especifíca; Fil: Porfiri, Alejandro. No especifíca; Fil: Fernández, Paula del Carmen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Scandiani, María Mercedes. Universidad Nacional de Rosario; Argentina Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Fitopatología; Argentina

Congenital adrenal hyperplasia (CAH) consists of several autosomal recessive disorders that inhibit steroid biosynthesis. We describe a case report diagnosed with adrenal insufficiency due to low adrenal steroids and adrenocorticotropic hormone excess due to lack of cortisol negative feedback signaling to the pituary gland. Genetic work up revealed two missense variants, p.Thr204Arg and p.Leu260Arg in the STAR gene, inherited by both parents (non-consanguineous). The StAR protein supports CYP11A1 enzyme to cleave the side chain of cholesterol and synthesize pregnenolone which is metabolized to all steroid hormones. We used bioinformatics to predict the impact of the variants on StAR activity and then we performed functional tests to characterize the two novel variants. In a cell system we tested the ability of variants to support cholesterol conversion to pregnenolone and measured their mRNA and protein expression. For both variants, we observed loss of StAR function, reduced protein expression and categorized them as pathogenic variants according to guidelines of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. These results fit the phenotype of the girl during diagnosis. This study characterizes two novel variants and expands the list of missense variants that cause CAH.

This study analyzes the effectiveness of different information criteria for the selection of covariance structures, extending it to different missing data mechanisms, the maintenance and adjustment of the mean structures, and matrices.The Monte Carlo method was used with 1,000 simulations, SAS 9.4 statistical software and a partially repeated measures design (p=2; q=5). The following variables were manipulated: a) the complexity of the model; b) sample size; c) matching of covariance matrices and sample size; d) dispersion matrices; e) the type of distribution of the variable; f) the non-response mechanism.The results show that all information criteria worked well in Scenario 1 for normal and non-normal distributions with heterogeneity of variance. However, in Scenarios 2 and 3, all were accurate with the ARH matrix, whereas AIC, AICCR and HQICR worked better with TOEP and UN. When the distribution was not normal, AIC and AICCR were only accurate in Scenario 3, more heterogeneous and unstructured matrices, with complete cases, MAR and MCAR.In order to correctly select the matrix it is advisible to analyze the heterogeneity, sample size and distribution of the data.

RESUMEN En el contexto de la ciencia abierta, la gestión, el intercambio y el reúso de datos científicos se han convertido en una práctica tan presente en los procesos de investigación, que actualmente se considera un elemento inherente al oficio del investigador. Para la realidad de Colombia, este es un tema relativamente nuevo en la práctica científica nacional. La ausencia de trabajos académicos que revelen el estado actual del contexto científico colombiano en relación con este tema, generó el siguiente cuestionamiento: ¿cómo los investigadores, adjuntos a los grupos de investigación clasificados por Colciencias, en el área de economía y negocios, practican la gestión, intercambio y reúso de datos de investigación? Para desvendar este fenómeno, la investigación adoptó un enfoque cuantitativo, de naturaleza descriptiva, bajo el método de investigación survey. El análisis de datos se realizó mediante estadística descriptiva y se interpretó bajo el marco teórico establecido. Se concluyó que los investigadores no aplican secuencialmente el ciclo de vida de los datos, usando apenas parte de sus fases, al igual que parte de las actividades que conforman las fases implementadas. En ese aspecto, se evidenció que las prácticas de gestión de datos que con mayor ocurrencia realizan los investigadores están implícitamente dentro del proceso tradicional de investigación. Ya las prácticas exclusivas de la curaduría como la documentación de un plan de gestión de datos, creación de metadatos, y preservación tienden a ser realizadas por los investigadores ocasionalmente. El grado de ocurrencia con que se implementa la gestión de datos descifró que las fases consideradas fundamentales para generar datos de investigación de calidad, en términos de curaduría, preservación y acceso abierto, no son constantes en la cotidianidad investigativa de los profesores. Por otra parte, la práctica de intercambiar datos abarca inúmeras formas que varían de investigador a investigador. El uso de datos propios generados en anteriores investigaciones y el reúso de datos de investigación bajo autoría de terceros, no es una práctica común entre los investigadores. En esa dirección se concibe que el acceso abierto a datos se desarrolla de modo informal y limitado al contexto y red profesional del investigador. Finalmente, la presente investigación es una contribución para futuramente trazar estrategias y acciones que permitan una mayor productividad investigativa y una buena gestión de los recursos científicos en el contexto estudiado. Nenhuma No contexto da ciência aberta, a gestão, compartilhamento e reúso de dados de pesquisa tornou-se uma prática tão presente nos processos de pesquisa que atualmente é vista como elemento inerente ao ofício do pesquisador. Para a realidade da Colômbia trate-se de um tema relativamente novo na prática científica nacional. A ausência de trabalhos acadêmicos que revelem o estado atual do contexto científico colombiano perante esse tema, permite que se questione: Como os pesquisadores, pertencentes a grupos de pesquisa classificados pela Colciencias na área de economia e negócios, praticam a gestão, compartilhamento e reúso de dados de pesquisa? Com o objetivo de desvendar tal fenômeno, a pesquisa adotou uma abordagem quantitativa, de natureza descritiva, sob o método de pesquisa Survey. A análise dos dados foi realizada através de estatística descritiva e foi interpretada sob o arcabouço teórico estabelecido. Concluiu-se que os pesquisadores não aplicam sequencialmente o ciclo de vida dos dados, utilizando apenas parte de suas fases, bem como parte das atividades que compõem as fases implementadas. Nesse aspecto, evidencia-se que as práticas de gestão de dados que os pesquisadores realizam com mais frequência encontram-se implicitamente dentro do processo tradicional de pesquisa. Já as práticas exclusivas da curadoria, como documentação de um plano de gestão de dados, criação de metadados e preservação, tendem a ser realizadas ocasionalmente pelos pesquisadores. O grau de ocorrência com que a gestão de dados é implementada desvendou que as fases consideradas fundamentais para a geração de dados de pesquisa de qualidade, em termos de curadoria, preservação e acesso aberto, não são constantes no cotidiano investigativo dos professores. Por outro lado, a prática de compartilhamento de dados abrange inúmeros métodos que variam de pesquisador para pesquisador. O uso de dados próprios gerados em pesquisas anteriores e o reúso de dados de pesquisa sob autoria de terceiros não é uma prática comum entre os pesquisadores. Nessa direção, concebe-se que o acesso aberto a dados é desenvolvido de maneira informal e limitado ao contexto e à rede professional do pesquisador. Finalmente, a presente pesquisa é uma contribuição para a elaboração futura de estratégias e ações que permitam maior produtividade investigativa e boa gestão dos recursos científicos no contexto estudado.

In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina. Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Ríos. Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernandez, Paula del Carmen. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina

Peripheral membrane proteins are required for signal propagation upon ligand-induced receptor activation at the plasma membrane.The translocation of this amphitropic peripheral proteins from or to the plasma membrane enables signal cascade propagation into the cells. This translocationgreatly depends on the membrane’s lipid composition and, consequently, regulation of the lipid bilayer emerges as a novel therapeutic strategy. Indeed, relevant changes in membrane lipids can induce massive translocation of peripheral signaling proteins from or to the plasma membrane, which controls how cells behave. We called these changes “lipid switches”, as they alter the cell’s status (e.g., proliferation, differentiation, death, etc.) in response to the modulation of membrane lipids. This discovery enables therapeutic interventions focused on modifying the bilayer’s lipids, an approach known as membrane-lipid therapy (MLT) or melitherapy.

The relationship between biofilm formation and RpoS status was assessed in nine field isolates of C. sakazakii. Their ability to form biofilms was studied in BHI and minimum media with different pH values and supplemented or not with the amino acids arginine, lysine and glutamic acid. Biofilm formation, both on polystyrene and stainless steel, was measured by spectrometric determination of the fixed crystal violet and the biofilms were visualized by confocal laser scanning microscopy and scanning electron microscopy. Despite the existing heterogeneity among the different strains, biofilm formation was generally higher in buffered minimum media (pH 7.0) supplemented with lysine than in other culture media and on stainless steel plates than on polystyrene. The results showed a lower ability to form biofilms for a strain with a loss-of-function mutation in the rpoS gene, the general stress response regulator of Gram-negative bacteria, when compared to the rest of the strains, which harboured a functional rpoS. The complementation of this strain with a functional rpoS gene resulted in an increase in its biofilm formation ability up to levels comparable to those observed for strains with a functional rpoS. However, the differences were markedly reduced when the incubation time was increased from 24 to 48 h, indicating that the loss of RpoS caused a delay in the development of mature biofilms, rather than a complete inhibition of biofilm production in C. sakazakii.

The long-term managing from the geological hazard point of view of the Hontomín onshore pilot-plant for CO2 storage, located in Spain and recognized as the first and only key-test facility in Europe, is one of the main objectives stated in the ENOS European project. This project is led and funded by the European Network of Excellence on the Geological Storage of CO2 (CO2GeoNet).The complex geological emplacement of the Hontomín Carbon capture and storage plant is considered rather relevant to analyse the impact of fracturing and both local and regional strain field on the reservoir parameters. The reservoir of Hontomín pilot-plant is formed by highly fractured Middle Jurassic dolomites with associated secondary porosity. This parameter is one of the main concerns when managing CO2 storage and monitoring.In order to characterize the fracture pattern and its implications on a proper CO2 monitoring, we characterized the surface structural elements through the study area and their relationship with fractures affecting the reservoir porosity. The methodology followed in this work is mainly based on detailed geological mapping (field work complimented with orthophoto analysis), adding missing information from previous works. This analysis does not increase the cost for long-term monitoring, given that they are low-cost and the results are acquired in a few months.The main structural trend in the study area, concerning faults with a wide range of displacement and metric to decametric folds, follows a regional E-W orientation. On the other hand, fractures show two main sets of trends, from NW-SE to NE-SW.This fracturing pattern, considered as a conjugate fracture system, corresponds to the tectonic stress recorded in both Mesozoic and Cenozoic sedimentary successions where the Hontomín pilot-plant is placed. Riddle faults formed from a nearby regional right-lateral strike slip fault (Ubierna Fault) are the responsible structures for the fracture system affecting the area and the reservoir. Moreover, this fracturing pattern is in agreement with local to regional active tectonic field from Cenozoic times to present-day, when the Ubierna Fault recorded its maximum right-lateral displacement (15 km).Secondary porosity within the reservoir can be produced from this fracture pattern, highly increasing the permeable migration paths for CO2 migration after the injection. Therefore, we state that a combination between fracture analysis and structural and tectonic study, should be considered as mandatory in the monitoring phases of the CO2 plume, during and after injection operations.

Background Secondary hyperparathyroidism (SHPT) is a matter of concern after biliopancreatic diversion (BPD). The aim of this study was to investigate the relationship between SHPT, 25(OH)D, and calcium after BPD. Design A retrospective analysis in obese patients after BPD performed between 1998 and 2016. Methods Patients with at least 1 year of follow-up were included. SHPT was considered when PTH > 65 pg/mL in the absence of an elevated corrected calcium. 25(OH)D (ng/mL) status was defined as: deficiency Results In total, 321 patients were included (76.6% women), with mean age 43.0 (10.5) years. Median follow-up was 6.0 (IQR 3.0-9.0) years. Mean body mass index was 49.8 (7.0) kg/m2. SHPT increased to a maximum of 81.9% in the ninth year of follow-up (95% CI: 1.5-9.1). Two years after surgery, 33.9% of patients with 25(OH)D sufficiency had SHPT (p = 0.001). Corrected calcium levels were lower in patients with PTH > 65 pg/mL when compared with PTH 65 pg/mL and 25(OH)D sufficiency had lower corrected calcium levels when compared with subjects with PTH and 25(OH)D in normal range. Two years: 9.0 vs 9.2 mg/dL (p Conclusions Once 25(OH)D is sufficient, the increase in PTH persists associated with a decrease in serum corrected calcium. It is important to ensure a sufficient calcium intake in these patients in order to avoid SHPT and osteomalacia in the future.

Background: RUNX1 is a crucial transcription factor for hematological stem cells and well-known for its association with acute lymphoblastic leukemia (ALL) and acute myelogenous leukemia (AML). Besides the translocation t(8; 21) that leads to the RUNX1-RUNX1T1 fusion, somatic mutations of RUNX1 have been discovered. Methods: Four bone marrow trephine biopsies of patients with CD79a-positive and/or PAX5-positive acute leukemias were investigated by immunohistochemistry (IHC), karyotyping, and next-generation sequencing-based genetic analysis. Data were then compared to a historical collective of AML (n = 42) and 42 cases of AML newly diagnosed at our institution between June 2017 and May 2018. Results: We report on 4 cases of acute leukemia with an equivocal immunophenotype showing expression of CD79a and/or PAX5, which led to a preliminary histopathologic classification as probable ALL/unclassifiable acute leukemia. All cases were positive for CD34 and TdT but negative for several myeloid markers on IHC. Mutational analysis revealed point mutations and indels of RUNX1 and further mutations typical for AML such as TET2, DNMT3A, and SRSF2, and 2 cases had tetrasomy 13 characteristic of RUNX1 mutant AML. Conclusion: Aberrant CD79a and/or PAX5 expression can be found in AML cases with RUNX1 mutations even without the translocation t(8; 21). Our series shows the expression of CD79a and PAX5 to be a potential pitfall in the classification of RUNX1 mutant acute leukemia.

There are few studies that explore the involvement of the cerebellum in the affective area. This study aims to evaluate the affective component and, specifically, the emotional states of depression and anxiety associated with the Chiari malformation, controlling perceived pain. This study included a Chiari group and two control groups, one healthy, the other suffering from Myasthenia gravis, which exhibits a physical component without cerebellar impairment. Each participant completed the Hospital Anxiety and Depression Scale and the Numeric Pain Rating Scale. Scores were significantly higher for the anxiety variable in the Chiari group, either when compared to the control group or the group affected by Myasthenia gravis-controlling the level of headache. The results obtained support previous studies in which the important contribution of the cerebellum to emotional regulation was emphasized.

espanolLa malformacion de Chiari (MC) es una patologia rara de baja prevalencia y cuya patogenia actualmente sigue siendo objeto de debate. La MC esta dentro del grupo de las malformaciones de la union craneocervical, es una alteracion anatomica de la base craneal. A dia de hoy se considera que hay 6 tipos de MC, siendo la MC tipo I la mas comun. La sintomatologia puede ser muy variada habiendose reportado casos de pacientes totalmente asintomaticos y otros con graves afecciones de tipo neurologicas. Recientes investigaciones manifiestan que las patologias congenitas del cerebelo como la MC, pueden acompanarse de deficits neuropsicologicos. Es de dificil diagnostico debido a la complejidad de la lesion y sintomatologia presente en los pacientes. No obstante, el diagnostico se realiza mediante tecnicas de neuroimagen siendo la tecnica mas utilizada la resonancia magnetica. En cuanto al tratamiento, la cirugia es una de las principales actuaciones a llevar a cabo ante el diagnostico de MC. Esta revision presenta un analisis exhaustivo de la literatura existente sobre las funciones cognitivas afectadas de la MC tipo I. Asimismo, realiza una descripcion de los efectos de los tratamientos quirurgicos en la sintomatologia neuropsicologica. Los resultados evidencian la presencia de deficits cognitivos; sin embargo, no hay acuerdo sobre cuales son exactamente las funciones cognitivas afectadas. EnglishChiari malformation (CM) is a rare pathology with low prevalence. CM is within the group of malformations of the craniocervical junction, it is an anatomical alteration of the cranial base. There are considered to be 6 types of CM, being MC type I the most common one. The symptomatology can be very varied, having been reported cases of totally asymptomatic patients and others with serious neurological affections. Furthermore, recent research shows that congenital pathologies of the cerebellum such as CM can be accompanied by neuropsychological deficits. It is difficult to diagnose it due to the complexity of the lesion and the symptoms present in patients. However, the diagnosis is made by neuroimaging techniques, being the most widely used technique the magnetic resonance. Regarding to treatment, surgery is one of the main actions to be carried out after the diagnosis of CM. This review presents an exhaustive analysis of the existing literature on the cognitive functions affected of type I MC. It also describes the effects of surgical treatments on neuropsychological symptoms. The results show the presence of cognitive deficits; however, there is no agreement about exactly what cognitive functions are affected.

During pregnancy, thyroid peroxidase (TPO) antibodies may increase the risk of developing subclinical hypothyroidism (SCH). Both conditions appear to be associated to maternal-fetal complications. The objectives of this study were to analyze if a relationship exists between TSH and TPO levels during pregnancy and the potential effects on gestational and perinatal complications, and to assess whether detectable, but not positive, TPO levels have an impact on development of gestational SCH.A prospective study was conducted at the Leon Health Area (CAULE), where universal screening for gestational thyroid dysfunction is performed between weeks 7-13 of pregnancy. Data on TSH and TPO levels and gestational and perinatal complications were collected for all 2016 deliveries. Positive TPO antibodies were defined as values≥35IU/mL. In a previous study, a TSH level3.72mU/L was established as the cut-off value for gestational SCH.Records corresponding to 1,980 deliveries at CAULE, 21 abortions, and 18 deliveries outside the hospital were analyzed. Of the 1,670 pregnant women screened (84.34%), 142 (8.50%) had positive TPO antibodies and their presence was associated to diagnosis of SCH (P0.01) and to significantly higher mean TSH levels (3.51mU/L vs. 2.46mU/L, P=0.03). There were no significant differences in gestational or neonatal complications. In the group with undetectable TPO antibodies (10lU/mL), the mean TSH levels was slightly lower than in the group with TPO values ranging from 10-35 IU/mL, but the difference was not significant (P=0.89).Presence of positive TPO antibodies is associated to higher TSH levels and higher risk of gestational SCH, but does not increase the rate of maternal-fetal complications.

Resumen Introduccion Los anticuerpos antiperoxidasa tiroidea (ATPO) en la gestacion pueden influir en el desarrollo de hipotiroidismo subclinico gestacional (HSG). Ambas entidades parecen asociarse a complicaciones maternas y fetales. Los objetivos de este estudio son analizar si existe relacion entre los valores de TSH y ATPO durante el embarazo, los posibles efectos sobre complicaciones gestacionales y perinatales, y valorar si los ATPO detectables, pero no positivos, influyen en el desarrollo de HSG. Metodologia Estudio prospectivo realizado en el area sanitaria del Complejo Asistencial Universitario de Leon (CAULE), donde se realiza cribado universal para disfuncion tiroidea gestacional entre la semana 7-13 de gestacion. Se recogieron datos de TSH, ATPO, obstetricos y neonatales de los partos de 2016. Se considera ATPO positivo si ≥ 35 UI/ml. En estudio previo se establecio valor TSH > 3,72 mU/L como corte para HSG. Resultados Se analizaron registros correspondientes a 1.980 partos en CAULE, 21 abortos y 18 partos fuera del centro. Se realizo cribado a 1.670 gestantes (84,34%): 142(8,50%) tuvieron ATPO positivos. La deteccion de ATPO positivo se asocio con el diagnostico de HSG (p Conclusion La presencia de ATPO positivo se asocia con valores de TSH mas elevados y con mayor riesgo de HSG, pero no incrementa la tasa de complicaciones materno-fetales.

Background: In 2013 the Chilean regulatory sanitary agency issued a warning concerning dose adjustment and use restriction to avoid severe adverse effects of metoclopramide such tardive dyskinesia. Aim: To study dyskinesia type adverse effects in a population using metoclopramide. Material and Methods: A cross sectional observational study was conducted among patients pertaining to palliative care and diabetes mellitus programs and consuming 10 mg/day or more of metoclopramide. Patients were interrogated looking for extrapiramidal signs and symptoms using a questionnaire validated by two neurologists. Results: In 40% of diabetic patients with gastroparesia and 35% of palliative care patients, extrapyramidal adverse reactions to metoclopramide were suspected. Palliative Care patients suffered the largest number of adverse events. The period of use and individual doses of the drug were largely above Chilean regulatory agency recommendations in all cases. Conclusions: A significant number of patients using metoclopramide could experience extrapyramidal adverse reactions.

The transcription factor SOX18 has been shown to play a role in the development of hair, blood and lymphatic vessels. Mutations in SOX18 result in hereditary lymphedema, with the unique clinical association of hypotrichosis and telangiectasia (HLTS). Some patients present with additional disease features which may be explained by the location of SOX18 mutation. We report a patient with hypotrichosis-lymphedema-telangiectasia syndrome (HLTS) confirmed by detection of a novel mutation in the SOX18 gene. Few cases of HTLS have been reported in the literature. We reviewed all cases reported to date to delineate the clinical manifestations that allow us to prompt diagnosis of this syndrome for appropriate management and genetic counseling.