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Implications of building codes upon design and construction of recreation facilities are investigated (national building codes, recreation facility standards, and misperceptions of design requirements). Recreation professionals can influence architectural designers to correct past deficiencies, but they must understand architectural and engineering factors in order to be administrators of facility projects. (SM)

Pyrodiversity, which describes fire variability over space and time, is believed to increase habitat heterogeneity and thereby promote biodiversity. However, to date there is no standardised metric for quantifying pyrodiversity, and so broad geographic patterns and drivers of pyrodiversity remain unexplored. We present the first generalizable method to quantify pyrodiversity, and use it to address the fundamental questions of what drives pyrodiversity, which fire attributes constrain pyrodiversity under different conditions, and whether pyrodiversity is spatial grain‐dependent. We linked the MODIS burned area and active fire products to measure fire size, seasonal timing, return interval, and intensity for 2.2 million individual fires in sub‐Saharan Africa from 2000–2015. We then quantified pyrodiversity as a four‐dimensional hypervolume described by fire attributes within a grid cell, for any spatial grain of analysis. Environmental (rainfall, vegetation, soils, and topography) and human‐associated (cattle biomass, cropland area, and human population density) variables were assessed as potential drivers of pyrodiversity. Rainfall was the main environmental driver of pyrodiversity, with higher pyrodiversity in drier regions (< 650 mm yr–1). Pyrodiversity was not strongly associated with human‐associated variables across Africa. Rainfall and a human influence index had clear but contrasting effects on the variability of fire size, seasonal timing, return interval, and intensity. Our analyses show that fire size and seasonal timing constrain pyrodiversity in wetter regions, whereas none of the fire attributes pose a strong constraint in drier regions. We found no evidence that pyrodiversity was spatial grain‐dependent when recalculated at 5‐minute grain increments from 15 to 120 minutes. We hypothesise that the strongest positive effect of pyrodiversity on biodiversity in all its forms will occur at intermediate precipitation (650–1300 mm yr–1), where fire plays an important role in shaping vegetation structure and where pyrodiversity is still quite high. [ABSTRACT FROM AUTHOR]

Abstract: Conservation management is strongly shaped by the interpretation of population trends. In the Serengeti ecosystem, Tanzania, aerial total counts indicate a striking increase in elephant abundance compared to all previous censuses. We developed a simple age‐structured population model to guide interpretation of this reported increase, focusing on three possible causes: (1) in situ population growth, (2) immigration from Kenya, and (3) differences in counting methodologies over time. No single cause, nor the combination of two causes, adequately explained the observed population growth. Under the assumptions of maximum in situ growth and detection bias of 12.7% in previous censuses, conservative estimates of immigration from Kenya were between 250 and 1,450 individuals. Our results highlight the value of considering demography when drawing conclusions about the causes of population trends. The issues we illustrate apply to other species that have undergone dramatic changes in abundance, as well as many elephant populations. [ABSTRACT FROM AUTHOR]

Rationale: Acute respiratory distress syndrome (ARDS) remains a major cause of respiratory failure in critically ill patients. Mesenchymal stromal cells (MSCs) are a promising candidate for a cell-based therapy. However, the mechanisms of MSCs' effects in ARDS are not well understood. In this study, we focused on the paracrine effect of MSCs on macrophage polarization and the role of extracellular vesicle (EV)-mediated mitochondrial transfer.Objectives: To determine the effects of human MSCs on macrophage function in the ARDS environment and to elucidate the mechanisms of these effects.Methods: Human monocyte-derived macrophages (MDMs) were studied in noncontact coculture with human MSCs when stimulated with LPS or bronchoalveolar lavage fluid (BALF) from patients with ARDS. Murine alveolar macrophages (AMs) were cultured ex vivo with/without human MSC-derived EVs before adoptive transfer to LPS-injured mice.Measurements and Main Results: MSCs suppressed cytokine production, increased M2 macrophage marker expression, and augmented phagocytic capacity of human MDMs stimulated with LPS or ARDS BALF. These effects were partially mediated by CD44-expressing EVs. Adoptive transfer of AMs pretreated with MSC-derived EVs reduced inflammation and lung injury in LPS-injured mice. Inhibition of oxidative phosphorylation in MDMs prevented the modulatory effects of MSCs. Generating dysfunctional mitochondria in MSCs using rhodamine 6G pretreatment also abrogated these effects.Conclusions: In the ARDS environment, MSCs promote an antiinflammatory and highly phagocytic macrophage phenotype through EV-mediated mitochondrial transfer. MSC-induced changes in macrophage phenotype critically depend on enhancement of macrophage oxidative phosphorylation. AMs treated with MSC-derived EVs ameliorate lung injury in vivo. [ABSTRACT FROM AUTHOR]

Arboviruses are public health threats that cause explosive outbreaks. Major determinants of arbovirus transmission, geographic spread, and pathogenesis are the magnitude and duration of viremia in vertebrate hosts. Previously, we determined that multiple alphaviruses are cleared efficiently from murine circulation by the scavenger receptor MARCO (Macrophage receptor with collagenous structure). Here, we define biochemical features on chikungunya (CHIKV), o'nyong 'nyong (ONNV), and Ross River (RRV) viruses required for MARCO-dependent clearance in vivo. In vitro , MARCO expression promotes binding and internalization of CHIKV, ONNV, and RRV via the scavenger receptor cysteine-rich (SRCR) domain. Furthermore, we observe species-specific effects of the MARCO SRCR domain on CHIKV internalization, where those from known amplification hosts fail to promote CHIKV internalization. Consistent with this observation, CHIKV is inefficiently cleared from the circulation of rhesus macaques in contrast with mice. These findings suggest a role for MARCO in determining whether a vertebrate serves as an amplification or dead-end host following CHIKV infection. [Display omitted] • Specific biochemical features of alphaviruses facilitate MARCO-dependent clearance in mice • Murine MARCO binds and internalizes CHIKV, ONNV, and RRV via the SRCR domain • MARCO SRCR domains from ungulates, but not potential amplification hosts, internalize CHIKV • Unlike mice, rhesus macaques inefficiently clear CHIKV after intravenous inoculation Li et al. find that murine MARCO enhances alphavirus internalization and limits viremia based on specific virion features. Although MARCO from several species promotes CHIKV internalization, MARCO from known or potential CHIKV-amplifying hosts does not, and rhesus macaques failed to clear CHIKV from blood circulation. These findings suggest MARCO impacts CHIKV transmission. [ABSTRACT FROM AUTHOR]

Context: Managers are faced with numerous methods for delineating wildlife movement corridors, and often must make decisions with limited data. Delineated corridors should be robust to different data and models. Objectives: We present a multi-method approach for delineating and validating wildlife corridors using multiple data sources, which can be used conserve landscape connectivity. We used this approach to delineate and validate migration corridors for wildebeest ( Connochaetes taurinus) in the Tarangire Ecosystem of northern Tanzania. Methods: We used two types of locational data (distance sampling detections and GPS collar locations), and three modeling methods (negative binomial regression, logistic regression, and Maxent), to generate resource selection functions (RSFs) and define resistance surfaces. We compared two corridor detection algorithms (cost-distance and circuit theory), to delineate corridors. We validated corridors by comparing random and wildebeest locations that fell within corridors, and cross-validated by data type. Results: Both data types produced similar RSFs. Wildebeest consistently selected migration habitat in flatter terrain farther from human settlements. Validation indicated three of the combinations of data type, modeling, and corridor detection algorithms (detection data with Maxent modeling, GPS collar data with logistic regression modeling, and GPS collar data with Maxent modeling, all using cost-distance) far outperformed the other seven. We merged the predictive corridors from these three data-method combinations to reveal habitat with highest probability of use. Conclusions: The use of multiple methods ensures that planning is able to prioritize conservation of migration corridors based on all available information. [ABSTRACT FROM AUTHOR]

Tree recruitment in savannas proceeds in multiple stages characterized by successive filters occurring at the seed and seedling stages. The 'demographic bottleneck' hypothesis suggests that such filters ultimately restrict tree density and prevent trees from dominating grasses in savannas, but many of the demographic transitions underlying this assumption have not been quantified. We investigated how short- (1-2 years) and long-term (40 + years) rainfall patterns influenced seed production, infestation, and viability for two dominant species, Acacia robusta and Acacia tortilis across the Serengeti ecosystem mean annual precipitation gradient over a two-year period. We found that neither production, nor infestation, nor viability was influenced by rainfall. Pod production differed between species and increased with tree height in A. robusta. Mean infestation proportion in 2013 was higher (mean ± SE; 0.28 ± 0.08) in A. tortilis than in A. robusta (0.11 ± 0.05) but the trend reversed in 2014, when A. tortilis (0.33 ± 0.10) had lower infestation than A. robusta (0.61 ± 0.09). Under laboratory conditions, A. tortilis and A. robusta seeds had maximum germination (= viability) proportions of 70 and 20%, respectively. Mean seed viability was more than five-fold higher (0.46 ± 0.19) in A. tortilis than in A. robusta (0.08 ± 0.10). Our study has produced important estimates for seed stage demographic dynamics that can be used for modeling tree dynamics in Serengeti system, and savannas in general. [ABSTRACT FROM AUTHOR]

Chikungunya virus (CHIKV) is a reemerging alphavirus that causes acute febrile illness and severe joint pain in humans. Although acute symptoms often resolve within a few days, chronic joint and muscle pain can be long lasting. In the last decade, CHIKV has caused widespread outbreaks of unprecedented scale in the Americas, Asia, and the Indian Ocean island regions. Despite these outbreaks and the continued expansion of CHIKV into new areas, mechanisms of chikungunya pathogenesis and disease are not well understood. Experimental animal models are indispensable to the field of CHIKV research. The most commonly used experimental animal models of CHIKV infection are mice and nonhuman primates; each model has its advantages for studying different aspects of CHIKV disease. This review will provide an overview of animal models used to study CHIKV infection and disease and major advances in our understanding of chikungunya obtained from studies performed in these models. [ABSTRACT FROM AUTHOR]

Adolescent Syrian hamsters ( Mesocricetus auratus ) treated with anabolic/androgenic steroids display increased offensive aggression and decreased anxiety correlated with an increase in vasopressin afferent development, synthesis, and neural signaling within the anterior hypothalamus. Upon withdrawal from anabolic/androgenic steroids, this neurobehavioral relationship shifts as hamsters display decreased offensive aggression and increased anxiety correlated with a decrease in anterior hypothalamic vasopressin. This study investigated the hypothesis that alterations in anterior hypothalamic vasopressin neural signaling modulate behavioral shifting between adolescent anabolic/androgenic steroid-induced offensive aggression and anxiety. To test this, adolescent male hamsters were administered anabolic/androgenic steroids and tested for offensive aggression or anxiety following direct pharmacological manipulation of vasopressin V1A receptor signaling within the anterior hypothalamus. Blockade of anterior hypothalamic vasopressin V1A receptor signaling suppressed offensive aggression and enhanced general and social anxiety in hamsters administered anabolic/androgenic steroids during adolescence, effectively reversing the pattern of behavioral response pattern normally observed during the adolescent exposure period. Conversely, activation of anterior hypothalamic vasopressin V1A receptor signaling enhanced offensive aggression in hamsters exposed to anabolic/androgenic steroids during adolescence. Together, these findings suggest that the state of vasopressin neural development and signaling in the anterior hypothalamus plays an important role in behavioral shifting between aggression and anxiety following adolescent exposure to anabolic/androgenic steroids. [ABSTRACT FROM AUTHOR]