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A variety of triangular wavelike FeNi films have been obtained on the annealed single-crystalline Al2O3 substrate by magnetron sputtering and photolithography. Nanoscale patterns and microscale stripes in double-layer FeNi nanofilm have also been studied. Properties relating to the morphology, and static and dynamic magnetic properties of materials were measured by AFM, SEM, vibrating sample magnetometers, and vector network analyzers. The surface of the annealed sample was wavy, and its wavelength and peak were 80 nm and 15.4 nm, respectively. Lithography formed 15-μm-wide lines on the surface. Compared with nano-scale patterns, the micro-scale stripes, and overlay patterns, fr increased to 4.5 GHz after superposition and FeNi was used as a soft magnetic material. Hc should have been small but, after superimposing the pattern, increased significantly. Hc along the easy axis increased to 18 Oe and that along the hard axis to 50 Oe. This was related to the coupling effect of the multilayer film.

In this paper, the microstructure evolution of the rapidly solidified (RS) Mg61.7Zn34Gd4.3 (at%, atomic ratio) alloy at high temperatures was investigated. The hardness and elastic modulus of the main precipitated phases were also analyzed and compared with those of the α-Mg matrix on the basis of nanoindentation tests. The results show that the RS alloy consists of either a petal-like icosahedral quasicrystal (IQC) phase (~20 μm) and block-shaped H1 phase (~15 μm) or IQC particles with an average grain size of ~107 nm as well as a small proportion of amorphous phase, which mainly depends on the holding time at the liquid temperature and the thickness of the ribbons. The IQC phase gradually transforms at 400°C to a short-rod-shaped μ-phase (Mg28.6Zn63.8Gd7.7) with a hexagonal structure. The hardness of the IQC phase is higher than that of H1 phase, and both phases exhibit a higher hardness than the α-Mg matrix and the μ-phase. The elasticity of the H1 phase is superior to that of the α-Mg matrix. The IQC phase possesses a higher elastic modulus than H1 phase. The easily formed H1 phase exhibits the poorest plastic deformation capacity among these phases but a higher elastic modulus than the α-Mg matrix.

The CoNi2S4/CdWO4-3 (CNS/CW-3) was an excellent photocatalyst for hydrogen evolution. The controlled morphology of CNS/CW-3 was successfully by adding thiourea as sulfur source and TBA to control the CoNi2S4 nanoparticles. The photocatalytic hydrogen evolution activity of CoNi2S4 and CdWO4 under visible light irradiation was studied with different CoNi2S4 incorporation ratios. UV–vis absorption spectra showed that the absorbance of CNS/CW-3 composite catalyst increased significantly after doping CoNi2S4. The electrochemical and photoelectrochemical experiments also showed that the charge separation and electron transfer efficiency were higher when CoNi2S4 was attached to CdWO4. CNS/CW-3 exhibited excellent photocatalytic activity for hydrogen evolution under visible light irradiation. Its hydrogen evolution activity reached 269.08 μmol in 5 h, and had good light stability. It was a potential strategy to design photocatalyst with controllable morphology.

Background and objectives: Ovarian cancer is the most fatal primary malignancy among gynecological cancers. Circular RNAs (circRNAs) play an important role in the development of various cancers, but the functions of circRNAs in ovarian cancer development have not been studied. We aim to explore the function and mechanism of CDR1as in the progression of ovarian cancer and to provide a new target for the diagnosis and treatment of ovarian cancer. Methods: Ovarian cancer cell proliferation was assessed according to proliferating cell nuclear antigen (PCNA) and Ki67 protein expression levels and MTT and CCK8 assays. The migration ability of cells was detected by scratch-wound assays, and the invasion ability of the cells was determined by Transwell® assays. qRT-PCR and Western blotting were used to verify miRNA and protein expression. Results: CDR1as expression in ovarian tissues was significantly lower in ovarian cancer patients than in patients without ovarian cancer. CDR1as overexpression inhibited the proliferation, invasion and migration of ovarian cancer cells. Silencing CDR1as increased the expression of miR-135b-5p and decreased the expression of hypoxia-inducible factor 1-alpha inhibitor (HIF1AN), thus increasing the proliferation capacity of ovarian cancer cells. Conclusions: CDR1as, acting as a sponge of miR-135b-5p, promotes the expression of HIF1AN and therefore plays a role in tumor inhibition.

A semisolid slurry of 7075 aluminum alloy was prepared by means of a serpentine channel process (SCP) and rheo-diecasting. The influences of pouring temperature during slurry preparation, the injection pressure and die preheat temperature on the mechanical properties and microstructure of a rheo-diecast 7075 aluminum alloy were investigated. The results show that the as-cast strength and elongation of the tensile samples were 210-260 MPa and 0.2%-1.7%, respectively, with an injection pressure of 130 MPa, a pouring temperature of 700-720 ºC, a preheat temperature of 280-350 ºC, and a plunger speed of 0.5 m·s-1. The results also show that the aged strength and elongation of the tensile samples were 420-453 MPa and 1.0%-1.4%, respectively, when the alloy solution was treated at 470 ºC for 12 h and aged at 120 ºC for 24 h. The substantial shrinkage porosity in the 7075 aluminum alloy tensile samples was the main cause of low elongation.

Osteosarcoma is the most common primary malignant bone tumor. Raddeanin A (RA) is an active oleanane-type triterpenoid saponin extracted from the traditional Chinese herb Anemone raddeana Regel that exerts antitumor activity against several cancer types. However, the effect of RA on osteosarcoma remains unclear. In the present study, we showed that RA inhibited proliferation and induced apoptosis of osteosarcoma cells in a dose- and time-dependent way in vitro and in vivo. RA treatment resulted in excessive reactive oxygen species (ROS) generation and JNK and ERK1/2 activation. Apoptosis induction was evaluated by the activation of caspase-3, caspase-8, and caspase-9 and poly-ADP ribose polymerase (PARP) cleavage. RA-induced cell death was significantly restored by the ROS scavenger glutathione (GSH), the pharmacological inhibitor of JNK SP600125, or specific JNK knockdown by shRNA. Additionally, signal transducer and activator of transcription 3 (STAT3) activation was suppressed by RA in human osteosarcoma, and this suppression was restored by GSH, SP600125, and JNK-shRNA. Further investigation showed that STAT3 phosphorylation was increased after JNK knockdown. In a tibial xenograft tumor model, RA induced osteosarcoma apoptosis and notably inhibited tumor growth. Taken together, our results show that RA suppresses proliferation and induces apoptosis by modulating the JNK/c-Jun and STAT3 signaling pathways in human osteosarcoma. Therefore, RA may be a promising candidate antitumor drug for osteosarcoma intervention.

By analyzing the cortical activation and functional connectivity of the prefrontal cortex (PFC) during dual-task obstacle negotiation in the older adults, cognitive resources allocation and neural regulatory mechanisms of aging brain were shed light on in complex walking conditions.Twenty-eight healthy right-handed subjects participated in the study, including 15 men and 13 women (age: 68.6 ± 4.1 years, height: 162.96 ± 6.05 cm, weight: 63.63 ± 9.64 kg). There were four tasks: Normal Walk (NW), Obstacle Negotiation during Normal Walk (NW + ON), Walk while performing Cognitive Task (WCT), and Obstacle Negotiation during Walk while performing Cognitive Task (WCT + ON). Participants wore functional near-infrared spectroscopy (fNIRS) to collect hemodynamic signals from various regions of interest (ROIs) in the PFC, while the three-dimensional motion capture system was used to test the gait velocity. Cognitive task data was recorded.In WCT + ON, the HbOAs walking difficulty increased, the PFC activation in the older adults changed from right-sided to bilateral activation, indicating that the left PFC cognitive resources compensated for the right PFC in dual-task obstacle negotiation. The cognitive resources recruitment in dual-task obstacle negotiation might be achieved by synchronization and coordination of associated brain areas in the PFC, primarily to maintain dynamic postural balance when walking.

Maternal caretaking and transport of dead infants are widespread among nonhuman primates, having been reported in numerous species of monkeys and apes. By contrast, accounts of such behaviors toward dead juveniles are scarce. Here, we describe responses by the mother and other group members to the death of a juvenile in a wild, multi-level group of Sichuan snub-nosed monkeys (Rhinopithecus roxellana). Following the juvenile’s fatal accident, his mother transported and cared for the corpse for four days. Immature monkeys belonging to the same one-male unit, and some individuals from other social units also showed interest in and tended the corpse. Comparisons of this case with those involving the deaths of infants and an adult female in the same population highlight possible effects of physiological, psychological and emotional factors in primate thanatological responses, and provide an additional perspective on the origin and evolution of compassionate acts.

Adenosine A(2A) receptors are G(olf)-coupled receptors and are predominantly expressed in the striatum of mammalian brains. As a mostly postsynaptic receptor, A(2A) receptors are implicated in the regulation of a variety of intracellular signaling pathways in striatopallidal output neurons and are linked to the pathogenesis of various neuropsychiatric and neurological disorders. This study investigated the possible role of A(2A) receptors in the modulation of the Src family kinase (SFK) in the adult rat striatum. In acutely prepared striatal slices, adding the A(2A) receptor agonist PSB-0777 induced a significant increase in phosphorylation of SFKs at a conserved autophosphorylation site (Y416) in the caudate putamen (CPu). This increase was also seen in the nucleus accumbens (NAc). Another A(2A) agonist CGS-21680 showed the similar ability to elevate SFK Y416 phosphorylation in the striatum. Treatment with the A(2A) receptor antagonist KW-6002 blocked the effect of PSB-0777 on SFK Y416 phosphorylation. In addition, PSB-0777 enhanced kinase activity of two key SFK members (Src and Fyn) immunoprecipitated from the striatum. These data demonstrate a positive linkage from A(2A) receptors to the SFK signaling pathway in striatal neurons. Activation of A(2A) receptors leads to the upregulation of phosphorylation of SFKs (Src and Fyn) at an activation-associated autophosphorylation site and kinase activity of these SFK members.

Rationale: Around 10%-20% patients with glioblastoma (GBM) are diagnosed with more than one tumor lesions or multifocal GBM (mGBM). However, the understanding on genetic, DNA methylomic, and transcriptomic characteristics of mGBM is still limited. Methods: In this study, we collected nine tumor foci from three mGBM patients followed by whole genome sequencing, whole genome bisulfite sequencing, RNA sequencing, and immunohistochemistry. The data were further examined using public GBM databases and GBM cell line. Results: Analysis on genetic data confirmed common features of GBM, including gain of chr.7 and loss of chr.10, loss of critical tumor suppressors, high frequency of PDGFA and EGFR amplification. Through profiling DNA methylome of individual tumor foci, we found that promoter methylation status of genes involved in detection of chemical stimulus, immune response, and Hippo/YAP1 pathway was significantly changed in mGBM. Although both CNV and promoter methylation alteration were involved in heterogeneity of different tumor foci from same patients, more CNV events than promoter hypomethylation events were shared by different tumor foci, implying CNV were relatively earlier than promoter methylation alteration during evolution of different tumor foci from same mGBM. Moreover, different tumor foci from same mGBM assumed different molecular subtypes and mesenchymal subtype was prevalent in mGBM, which might explain the worse prognosis of mGBM than single GBM. Interestingly, we noticed that LIF and CCL2 was tightly correlated with mesenchymal subtype tumor focus in mGBM and predicted poor survival of GBM patients. Treatment with LIF and CCL2 produced mesenchymal-like transcriptome in GBM cells. Conclusions: Together, our work herein comprehensively profiled multi-omics features of mGBM and emphasized that components of extracellular microenvironment, such as LIF and CCL2, contributed to the evolution and prognosis of tumor foci in mGBM patients.

Glioma stem cells (GSCs) are self-renewing tumor cells with multi-lineage differentiation potential and the capacity of construct glioblastoma (GBM) heterogenicity. Mitochondrial morphology is associated with the metabolic plasticity of GBM cells. Previous studies have revealed distinct mitochondrial morphologies and metabolic phenotypes between GSCs and non-stem tumor cells (NSTCs), whereas the molecules regulating mitochondrial dynamics in GBM cells are largely unknown. Herein, we report that carnitine palmitoyltransferase 1A (CPT1A) is preferentially expressed in NSTCs, and governs mitochondrial dynamics and GSC differentiation. Expressions of CPT1A and GSC marker CD133 were mutually exclusive in human GBMs. Overexpression of CPT1A inhibited GSC self-renewal but promoted mitochondrial fusion. In contrast, disruption of CPT1A in NSTCs promoted mitochondrial fission and reprogrammed NSTCs toward GSC feature. Mechanistically, CPT1A overexpression increased the phosphorylation of dynamin-related protein 1 at Ser-637 to promote mitochondrial fusion. In vivo, CPT1A overexpression decreased the percentage of GSCs, impaired GSC-derived xenograft growth and prolonged tumor-bearing mice survival. Our work identified CPT1A as a critical regulator of mitochondrial dynamics and GSC differentiation, indicating that CPT1A could be developed as a molecular target for GBM cell-differentiation strategy.

Major depressive disorder is a chronic psychiatric disease with a high prevalence. Brain mechanisms for depression at cellular and molecular levels are far from clear. Increasing evidence from clinical and preclinical studies reveals critical roles of the non-receptor tyrosine kinase (nRTK) superfamily in the pathophysiology, symptomatology, and therapy of depression. To date, several nRTK members from three nRTK subfamilies, i.e., the Src family kinase (SFK), the Janus tyrosine kinase (JAK) and the focal adhesion kinase (FAK) subfamilies, may connect to the intracellular, intranuclear, and synaptic signaling network linking chronic stress to depression- and anxiety-like behavior. These SFK/JAK/FAK nRTKs are abundantly expressed in the prefrontal cortex and hippocampus, two core limbic regions implicated in depression, and are enriched at synaptic sites. In various acute or chronic animal models of depression, the nRTKs were significantly altered (up- or downregulated) in their phosphorylation, expression, subcellular/subsynaptic distribution, and/or function. Stress that precipitates depressive behavior also influenced the interaction of nRTKs with other signaling molecules and downstream substrates, including ionotropic and metabotropic glutamate receptors. The commonly-used antidepressants showed the ability to alter nRTK activity. In sum, the limbic SFK/JAK/FAK nRTKs are sensitive to stress and undergo drastic adaptations in response to chronic depression. These long-lasting adaptations contribute to the remodeling of signaling network or synaptic plasticity critical for the vulnerability to depression and the therapeutic efficacy of antidepressants.

Objective: Uric acid is thought to be associated with the occurrence of atherosclerosis, which is closely related to cardio-cerebrovascular disease. However, the present study examined serum uric acid (SUA) and its correlation with carotid intima-media thickness (CIMT), which is a major issue. The purpose of this paper is to examine serum uric acid concentration and its correlation with carotid artery atherosclerosis according to age and sex groups.Methods: Individuals who underwent physical examinations at the First Affiliated Hospital of Chongqing Medical University from 2016 to 2020 were selected. The physical examination information of the subjects was recorded, and biochemical indexes such as blood uric acid levels were collected. The intima media thickness of the carotid artery was measured by ultrasound. Using traditional atherosclerosis risk factors as adjustment variables, the association between blood uric acid levels and atherosclerosis was assessed by logistic regression analysis.Results: A total of 15,843 subjects (73.90% male) were included, with an average age of 52±12 (20-92) years. The prevalence of CIMT thickened was 9.51%, and the plaque prevalence was 28.59%. Univariate analysis results showed that there were significant differences in CIMT thickening and plaque occurrence among different SUA level groups in both men and women (P430 μmol/L: OR=1.857, 95% CI: 1.264-2.73;P < 0.05. female: ≤210 μmol/L as the reference group, 260-310 μmol/L: OR=1.419, 95% CI: 1.059-1.901; 310-360 μmol/L: OR=1.432, 95% CI: 1.048-1.957; >360 μmol/L: OR=1.557, 95% CI: 1.113-2.177; P < 0.05). Correlation analysis in each age subgroup showed that CIMT was significantly associated with SUA in men ≥60 years old and women 45-60 years old and ≥60 years old (male: ≤220 μmol/L as the reference group, >430 μmol/L: OR=1.972, 95% CI: [1.2,3.238]. female: ≤210 μmol/L as the reference group, >360 μmol/L (45-60 years old): OR=1.77, 95% CI:[1.107,2.832]; > 360 μmol/L (≥60 years old), OR = 1.65, 95% CI: [1.067, 2.551]. P < 0.05).Conclusions: In both men and women, increased SUA levels are closely associated with thickened CIMT, which is associated with a higher risk of cardio-cerebrovascular disease. The age at which this association was observed in women was lower than in men, and whether this result is due to changes in hormone levels before and after menopause remains to be prospectively studied.

Rationale: The etiology of acute respiratory distress syndrome (ARDS) may play an important role in the failure of noninvasive ventilation (NIV). Objectives: To explore the association between ARDS...

Background Current guidelines indicate we can consider a bridging strategy that uses intravenous, reversible glycoprotein inhibitors for patients that required surgery following recent stent implantation. However, no strong clinical evidence exists that demonstrates the efficacy and safety of this treatment. Therefore, in this study, the efficacy and safety of a bridging strategy that uses intravenous platelet glycoprotein receptor inhibitors will be evaluated. Methods A meta-analysis was performed on preoperative bridging studies in patients undergoing coronary stent surgery. The primary outcome was the success rate of no major adverse cardiovascular events (MACE). The secondary outcomes were the success rate of no reoperations to stop bleeding. Results A total of 10 studies that included 382 patients were used in this meta-analysis. For the primary endpoint, the success rate was 97.7% (95% CI 94.4–98.0%) for glycoprotein IIb/IIIa inhibitors, 98.8% (95% CI 96.0–100%) for tirofiban (6 studies) and 95.8% (95% CI 90.4–99.4%) for eptifibatide (4 studies). For secondary endpoints, the success rate was 98.0% (95% CI 94.8–99.9%) for glycoprotein IIb/IIIa inhibitors, 99.7% (95% CI 97.1–100%) for tirofiban (5 studies), and 95.3% (95% CI 88.5–99.4%) for eptifibatide (4 studies). Conclusion The results of this study showed that the use of intravenous platelet glycoprotein IIb/IIIa inhibitors as a bridging strategy might be safe and effective for patients undergoing coronary stent implantation that require surgery soon after.

Background: Osteosarcoma (OS) is the most common malignant bone cancer. Recently, more studies have shown that TPX2, a potential oncogene considered to be an important prognostic biomarker, is widely expressed in various tumors. However, until now, little is known about the impacts of TPX2 on the pathogenesis of OS. Methods: TPX2 expression of in OS cells and tissues was detected by Western blot and immunohistochemical analyses. Various assays were used to analyze OS cell proliferation and metastasis in vitro and in vivo. The effect of TPX2 on the expression of E2F1 and proteins encoded by E2F1-regulated genes was evaluated by Western blotting. Coimmunoprecipitation, immunofluorescence and in vitro ubiquitination assays were employed to explore the molecular mechanisms underlying the effect of TPX2 on stabilizing E2F1 and maintaining E2F1 signaling. Findings TPX2 is overexpressed in OS and is significantly correlated with the malignant progression of OS. TPX2 probably plays a critical role in the proliferation and metastasis of OS cells in vitro. Furthermore, TPX2 regulates E2F1 signaling by affecting the stability of E2F1, thereby affecting the proliferation and metastasis of OS cells. In addition, PSMD14 impacts the degradation and regulates the stability of E2F1. Most importantly, TPX2 regulates the interaction of E2F1 and PSMD14 to influence E2F1 degradation. Interpretation: Collectively, our research not only identifies TPX2 ground as a promising therapeutic target in cancer but also illuminates its underlying molecular mechanism. We showed that TPX2 promotes the malignant progression of OS by regulating E2F1 signaling. Funding: This project was supported by the NSFC (81802985), and a Research Grant from the Shanghai Hospital Development Center (SHDC2020CR2037B). Declaration of Interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Ethical Approval: All animal experiments were performed under the protocols approved by the Shanghai General Hospital Institutional Animal Care and Use Committee. Six-week-old female BABL/c nude mice were obtained from SLRC laboratory Animal (Shanghai, China). The Central Laboratory of Shanghai General Hospital approved this study.

The goal of this investigation was to measure and analyze the root position of palatally malposed maxillary lateral incisor based on cone-beam CT(CBCT)images, in order to provide references for orthodontists to move this kind of teeth scientifically.CBCT data from 200 patients meeting the selection criteria with palatally malposed maxillary lateral incisor were investigated in this study. The root was divided into eight equal parts by length, then T1 to T8 were orderly pointed from root apex to alveolar ridge crest. The labial and palatal bone thickness at each point was measured; meanwhile, the angle between the long axis of the tooth and that of the alveolar bone was measured.The data was analyzed using SPSS 19.0 software package.The mean labial bone thicknesses at all researched points were less than 1.00 mm, except for point T1,T7 and T8. The mean labial bone thicknesses at point T3, T4 and T5 were the thinnest, which were all less than 0.5 mm(P0.05). The mean thicknesses of labial bone gradually increased from T4 to T8(P0.05). The mean palatal bone thicknesses were all more than 1.00 mm at the eight points,the mean thicknesses of palatal bone gradually increased from T8 to T1(P0.05). All the angulations between the long axes of teeth and those of the alveolar bone were negative, indicating the root was close to the labial alveolar wall. The average angulation was minus 31.06 degrees.Results suggest that the root of palatally malposed maxillary lateral incisor is close to the labial wall of the alveolar bone, its labial alveolar bone is frequently quite thin or even deficient, especially in the zone between 1/4 root length to the root apex and the mid-root. If we move the palatally malposed maxillary lateral incisor labially, it is better to choose tipping movement, instead of bodily movement, in order to avoid serious bone fenestration and dehiscence or root absorption.

In this study, we aimed to investigate treatment options and the prognosis of patients with WM in China. This retrospective study included 1141 patients diagnosed with symptomatic WM between January 2003 and December 2019 at 35 tertiary hospitals in 22 provinces of China. Fifty-four patients (7.3%) received monotherapy, 264 (36.0%) received chemoimmunotherapy, 395 (53.8%) received other combination regimens without rituximab, and 21 (2.9%) received ibrutinib. Using a multivariable Cox regression model, age > 65 years old, platelets 65 years, LDH ≥250 IU/L, ALB

Background The National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) was developed to accurately assess the pain, urinary symptoms, and quality of life related to chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). This study aimed to evaluate the cross-cultural adaptations of the NIH-CPSI. Method PubMed, Embase, CINAHL, and SciELO databases were searched from their established year to September 2020. Cross-cultural adaptations and the quality control of measurement properties of adaptations were conducted by two reviewers independently according to the Guidelines for the Process of Cross-Cultural Adaptation of Self-Report Measures and the Quality Criteria for Psychometric Properties of Health Status Questionnaire. Results Area total of 21 papers with 16 adaptations, and six studies of the original version of the NIH-CPSI were enrolled in the systematic review. Back translation was the weakest process for the quality assessment of the cross-cultural adaptations of the NIH-CPSI. Internal consistency was analyzed for most of the adaptations, but none of them met the standard. Only 11 adaptations reported test reliability, then only the Arabic-Egyptian, Chinese-Mainland, Danish, Italian, Persian, and Turkish adaptations met the criterion. Most adaptations reported the interpretability, but only the Danish adaptation reported the agreement. The other measurement properties, including responsiveness, and floor as well as ceiling effects were not reported in any of the adaptations. Conclusions The overall quality of the NIH-CPSI cross-cultural adaptations was not organized as expected. Only the Portuguese-Brazilian, Italian, and Spanish adaptations reached over half the process for the cross-cultural adaptation. Only the Turkish adaptations finished half of the measurement properties of cross-cultural adaptations.

Background As a new noninvasive diagnostic technique, computed tomography (CT)-based fraction flow reserve (FFR) has been used to identify hemodynamically significant coronary artery stenosis. This meta-analysis used invasive FFR as the standard to evaluate the diagnostic performance of FFRCT. Methods We searched the PubMed, Cochrane library, and EMBASE for articles published between January 2009 and January 2021. The synthesized sensitivity and specificity of invasive FFR and FFRCT were analyzed at both the patient and vessel levels. We generated a summary receiver operating characteristic curve (SROC) and then calculated the area under the curve (AUC). Results We included a total of 23 studies, including 2,178 patients and 3,029 vessels or lesions. Analysis at each patient level demonstrated a synthesized sensitivity of 88%, specificity of 79%, LR+ of 4.16, LR-of 0.15, and AUC of 0.89 for FFRCT. Analysis at the level of each vessel or lesion showed a synthesized sensitivity of 85%, specificity of 81%, LR+ of 4.44, LR-of 0.19, and AUC of 0.87 for FFRCT. Conclusion Our research reveals that FFRCT has high diagnostic performance in patients with coronary artery stenosis, regardless of whether it is at the patient level or the vessel level.

The ADJUVANT study reported the comparative superiority of adjuvant gefitinib over chemotherapy in disease-free survival (DFS) of resected EGFR-mutant stage II-IIIA non-small cell lung cancer (NSCLC). However, not all patients experienced favorable clinical outcomes with TKI, raising the necessity for further biomarker assessment. By comprehensive genomic profiling of 171 tumor tissues from the ADJUVANT trial, five predictive biomarkers were identified (TP53 exon4/5 mutations, RB1 alterations, and copy number gains of NKX2-1, CDK4, and MYC. Then we integrated them into the Multiple-gene INdex to Evaluate the Relative benefit of Various Adjuvant therapies (MINERVA) score, which categorized patients into three subgroups with relative disease-free survival and overall survival benefits from either adjuvant gefitinib or chemotherapy (Highly TKI-Preferable, TKI-Preferable, and Chemotherapy-Preferable groups). This study demonstrates that predictive genomic signatures could potentially stratify resected EGFR-mutant NSCLC patients and provide precise guidance towards future personalized adjuvant therapy.

This study investigated the effects of a 16-week Tai Chi (TC) intervention on cutaneous sensitivity and proprioception among older adults with and without sensory loss. Thirty-six older adults were divided into sensory loss and control groups, and they underwent a 16-week TC intervention. Significant interactions were detected in heel cutaneous sensitivity (

Time to stabilisation (TTS) provides limited information to evaluate the dynamic postural control for individuals with functional ankle instability in single-leg landing task. More information is needed to understand TTS and evaluate the dynamic postural control better. The purpose is to develop a method estimating TTS, initial impact force (IIF) and landing leg stiffness (LLS) through fitting ground reaction force (GRF) decay in single leg landing with exponential vibration decay function (EVDF), and investigate effects of landing directions and GRF components on these parameters. Thirty-nine healthy participants were recruited. EVDF was used to fit GRF decays in different single-leg landings. TTS, IIF, and LLS were compared across landing directions and GRF components. The mean regression determinants of fitting GRF decays with EVDF were greater than 0.50. TTS was sensitive to GRF components (P = 0.041 females, P = 0.028 males). IIF was sensitive to GRF components (P = 0.001) for both genders. LLS was sensitive to GRF components (P = 0.023) for males. This method showed a moderate to strong feasibility for reporting GRF decay during landing, and provided movement characteristic information for better understanding of dynamic postural control together with TTS during landing.

Background: The failure rate of noninvasive ventilation (NIV) remains high in patients with acute respiratory distress syndrome (ARDS). The etiology of ARDS may play an important role in NIV failure.Methods: A multicenter prospective observational study was performed in 17 ICUs in China from September 2017 to December 2019. ARDS patients who used NIV as a first-line therapy were enrolled. The etiology of ARDS was recorded at study entry. Results: A total of 306 patients were enrolled. Of the patients, 146 were classified as having pulmonary ARDS (ARDSp) and 160 were classified as having extrapulmonary ARDS (ARDSexp). NIV improved PaO2/FiO2 from initiation to 24 h of NIV in both groups. However, it improved more slowly in patients with ARDSp than in those with ARDSexp (interaction effect: p < 0.01). ARDSp patients experienced more NIV failure (55% vs. 28%; p < 0.01) and higher 28-day mortality (47% vs. 14%; p < 0.01). The multivariate Cox regression also showed that ARDSp was independently associated with NIV failure (hazard ratio [HR] = 2.81, 95% confidence interval [CI]: 1.89-4.18) and 28-day mortality (HR = 7.49, 95% CI: 4.32-13.01). After propensity matching, 62 patients remained in each group. The baseline data were comparable between the two groups. ARDSp was still independently associated with NIV failure and 28-day mortality (HR = 2.62, 95%CI: 1.49-4.61; and 5.70, 2.59-12.55, respectively). Sensitivity analysis also confirmed these results. Conclusions: Among ARDS patients who used NIV as a first-line therapy, ARDSp was associated with slower improvement in oxygenation, more NIV failure, and higher 28-day mortality than ARDSexp.

The aim of the present study was to investigate oral health status of the elderly in Shanghai using Eichner index dental classification system, and to discuss the relations between tooth loss, prosthodontic treatment and the impact on oral health-related quality of life(OHRQoL).A total of 747 subjects aging 65-74 years old were selected by multistage stratified cluster sampling method. Clinical examination and questionnaires of the Fourth National Oral Health Survey of China were used for the study, and data of tooth loss, OHRQoL were analyzed using non-parametric test and binary logistic regression with SPSS 19.0 software package.79.1% of the subjects had problem of missing teeth, while 35% of them never received any kind of dental prosthesis. Grouping by the Eichner index, only A1, A2 and C3 group were scored above the average, while group B had the lowest general oral health assessment index(GOHAI) score. The scores of GOHAI were significantly correlated to dental prosthodontic treatment, overall health status and Eichner index(P0.05). Except for group C2 due to the small sample size, the scores of GOHAI in most of the untreated subjects were lower than those who had prosthesis. Among the subjects with missing teeth but without prosthesis, 81.2% of the subjects thought they had no problem with their teeth or the problem did not matter.Tooth loss is a serious problem among the elderly in Shanghai, but only a few of them have received dental prosthodontic treatment. The OHRQoL of the elderly is related to tooth loss, dental prosthodontic treatment and overall health. Oral health education should be strengthened for the elderly people with missing teeth, to replace their missing teeth timely and improve their quality of life.

LiF is a low-firing fluoride with excellent microwave dielectric properties, however densification of LiF ceramics is challenging owing to their low surface free energy. In this study, a cold sintering process (150 °C, 250 MPa, 60 min) was employed to pre-densify LiF ceramics to 78 % relative density. Post-annealing treatments between 650 °C and 800 °C led to significant grain growth which was accompanied by an increase in relative density to 92 %. The microwave quality factor (Qf) increased with increasing annealing temperature to a maximum of 110,800 GHz at 800 °C, 1.5 times higher than the value obtained via conventional sintering (78,800 GHz), with relative permittivity εr = 8.2 and temperature coefficient of resonant frequency, τf =–135 ppm/°C. Such high values of Qf and its compatibility with Ag electrode suggest that cold sintered LiF has great potential as a component or additive in low temperature co-fired ceramic formulations.

Epilepsy is a chronic neurological disorder characterized by spontaneous recurrent seizures (SRS) and comorbidities. Kindling through repetitive brief stimulation of a limbic structure is a commonly used model of temporal lobe epilepsy. Particularly, extended kindling over a period up to a few months can induce SRS, which may simulate slowly evolving epileptogenesis of temporal lobe epilepsy. Currently, electroencephalographic (EEG) features of SRS in rodent models of extended kindling remain to be detailed. We explored this using a mouse model of extended hippocampal kindling. Intracranial EEG recordings were made from the kindled hippocampus and unstimulated hippocampal, neocortical, piriform, entorhinal, or thalamic area in individual mice. Spontaneous EEG discharges with concurrent low-voltage fast onsets were observed from the two corresponding areas in nearly all SRS detected, irrespective of associated motor seizures. Examined in brain slices, epileptiform discharges were induced by alkaline artificial cerebrospinal fluid in the hippocampal CA3, piriform and entorhinal cortical areas of extended kindled mice but not control mice. Together, these in vivo and in vitro observations suggest that the epileptic activity involving a macroscopic network may generate concurrent discharges in forebrain areas and initiate SRS in hippocampally kindled mice.

Background. Effective functional family was beneficial for older adults’ health, which may affect the quality of life (QoL) in hypertension patients. This study aimed to clarify the association between family function, anxiety, and QoL for older adults with hypertension in low-income communities. Methods. A questionnaire survey was conducted on 363 older adults with hypertension in low-income communities in Wuhan from September 2019 to November 2019. The relationships among the variables were examined by Pearson’s correlation analysis. Predictor effects were tested using hierarchical multiple regressions, controlling for demographic characteristics. The structural equation model (SEM) was used to test the mediation effects of anxiety on the pathway from family function to QoL. Results. Family function was negatively correlated with the self-rating anxiety scale (SAS) score and positively correlated with the mental component score (MCS), but had no influence on the physical component score (PCS). Both PCS and MCS were negatively correlated with SAS. Anxiety was the negative predictor of MCS and PCS. Family function was the positive predictor of MCS, but had no influence on PCS. The path model indicated that anxiety significantly mediated the link between family function and QoL (R2 = 32.8%), but only partially. Conclusion. A significant correlation between anxiety, family function, and QoL was found. Anxiety had a partial mediating effect on the relationship between family function and QoL. Further research should focus on increasing the level of family function and reducing the perceived anxiety of older adults with hypertension to improve their QoL level.

Data on the efficacy and safety of nonvitamin K antagonist oral anticoagulants (NOACs) in atrial fibrillation (AF) patients with cancer are limited. Therefore, we conducted a meta-analysis to compare the efficacy and safety between NOACs and warfarin in this population. A comprehensive search of the PubMed, Embase, and Cochrane databases for articles published through July 2020 was performed. An evaluation of each study was conducted, and data were extracted. Pooled odds ratio (OR) estimates and 95% CIs were calculated. Eight studies (3 randomized controlled trials (RCTs) and 5 retrospective cohort studies) involving a total of 24,665 patients were included. Among the RCTs, there were no significant differences in the rates of stroke or systemic embolism (OR=0.69; 95% CI, 0.45–1.06; P=0.09), venous thromboembolism (OR=0.91; 95% CI, 0.33–2.52; P=0.86), myocardial infarction (OR=0.74; 95% CI, 0.44–1.23; P=0.24), major bleeding (OR=0.81; 95% CI, 0.61–1.06; P=0.12), or major or nonmajor clinically relevant bleeding (OR= 0.98; 95% CI, 0.82–1.19; P=0.86) between the NOAC and warfarin groups. Among the observational studies, patients who used NOACs had a significantly lower risk than those who used warfarin. The prevalence rates of ischemic stroke (OR=0.51; 95% CI, 0.28–0.92; P=0.02), VTE (OR=0.50; 95% CI, 0.41–0.60; P

BACKGROUND Previous research suggests that formative assessment (FA) enhances learning outcomes, but few studies have evaluated its impact on clinical skills training in China. We conducted this study in a clinical skills integral curriculum to further explore the educational value of FA. MATERIAL AND METHODS Sixty undergraduates from the Second Clinical Medical School of the Southern Medical University in 2016 were selected as the experimental group (consecutive FA), and 50 undergraduates in 2015 were selected as the control term (only final summative assessment, SA). Undergraduates in the FA group completed the after-class questionnaire at each lesson. Teachers, teaching content, assessment objectives, and topics are the same in both groups. RESULTS The results of single-factor covariance (ANCOVA) analysis and Cochran-Mantel-Haenszel (CMH) analysis demonstrated that students of the FA group obtained better performance and higher success rates in summative examination than in the SA group. The students with relatively poor grades benefited more from FA, while the performance of students with higher grades was similar between the FA group and SA group. According to the results of questionnaire for students, the satisfaction of students with the course increased gradually, from 84.4% to 93.0%. CONCLUSIONS Proper use of FA is associated with better learning outcomes for students, especially for those with poorer grades. Our results, together with previous research, indicated that the use of FA may be of great benefit to students' academic performance and satisfaction with the clinical skills training curriculum.

PURPOSE Relapse is a major cause of treatment failure after allogeneic hematopoietic stem-cell transplantation (allo-HSCT) for high-risk acute myeloid leukemia (HR-AML). The aim of this study was to explore the effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) combined with minimal-dose decitabine (Dec) on the prevention of HR-AML relapse after allo-HSCT. PATIENTS AND METHODS We conducted a phase II, open-label, multicenter, randomized controlled trial. Two hundred four patients with HR-AML who had received allo-HSCT 60-100 days before randomization and who were minimal residual disease negative were randomly assigned 1:1 to either rhG-CSF combined with minimal-dose Dec (G-Dec group: 100 µg/m2 of rhG-CSF on days 0-5 and 5 mg/m2 of Dec on days 1-5) or no intervention (non–G-Dec group). The primary outcome was relapse after transplantation, and the secondary outcomes were chronic graft-versus-host disease (cGVHD), safety of the treatment, and survival. RESULTS The estimated 2-year cumulative incidence of relapse in the G-Dec group was 15.0% (95% CI, 8.0% to 22.1%), compared with 38.3% (95% CI, 28.8% to 47.9%) in the non–G-Dec group ( P < .01), with a hazard ratio (HR) of 0.32 (95% CI, 0.18 to 0.57; P < .01). There was no statistically significant difference between the G-Dec and non–G-Dec groups in the 2-year cumulative incidence of cGVHD without relapse (23.0% [95% CI, 14.7% to 31.3%] and 21.7% [95% CI, 13.6% to 29.7%], respectively; P = .82), with an HR of 1.07 (95% CI, 0.60 to 1.92; P = .81). After rhG-CSF combined with minimal-dose Dec maintenance, increasing numbers of natural killer, CD8+ T, and regulatory T cells were observed. CONCLUSION Our findings suggest that rhG-CSF combined with minimal-dose Dec maintenance after allo-HSCT can reduce the incidence of relapse, accompanied by changes in the number of lymphocyte subtypes.

Backgroud Family is the most important social support available to hypertensive patients, which may affect their health-related quality of life (HRQOL) and health outcomes. However, data on the relationship between family function and HRQOL among hypertensive residents are sparse, particularly for those low-income residents with hypertension. In this study, we aimed to examine the effects of family function on physical and mental health among low-income residents with hypertension in Central China, and to explore the independent contributions of socio-demographic variables, health-related factors and family function to each domain of HRQOL. Methods This cross-sectional, community-based survey, studied 295 low-income residents with hypertension. Family function was measured using the Family APGAR Index (Adaptation, Partnership, Growth, Affection, and Resolve). HRQOL was assessed using the SF-12 Questionnaire. Clustered multiple linear regressions were used to analyze the independent contributions of family function to each domain of HRQOL.Results 35.90% of low-income hypertensive residents had highly functional family. Multiple regression analyses showed that those with higher Family APGAR scores obtained higher general health (β=0.168, P=0.008), bodily pain(β=0.167, P=0.008), mental component summary (β=0.330, PP=0.022), mental health (β=0.302, PPPConclusion Family function was significantly associated with HRQOL among low-income hypertensive residents. This relation holds for both mental component summary and each of its individual domains, as well as partial physical domains.

OBJECTIVE To evaluate the distant metastatic patterns and prognostic factors for overall survival (OS) and cancer-specific survival (CSS) in de novo metastatic nasopharyngeal carcinoma (mNPC) using the Surveillance, Epidemiology, and End Results (SEER) database. METHODS Patients with de novo mNPC who had been diagnosed between 2004 and 2016 were identified from the SEER database. Kaplan-Meier analysis was used to calculate OS and CSS. Log-rank tests were employed to measure survival variation among subgroups. Individual predictors of CSS and OS were examined using Cox proportional-hazards regression models in patients with de novo mNPC. RESULTS We evaluated 224 patients with de novo mNPC who matched our inclusion criteria. Three-year CSS and OS for the whole cohort was 29.8% and 27.9%, respectively. Univariate analysis indicated that CSS and OS were influenced by age, histology, radiotherapy, chemotherapy, and liver metastasis. Neither the number of metastatic sites nor their specific location in bone, lungs, distant lymph nodes or brain significantly affected CSS or OS. The aforementioned independent prognosticators continued to significantly influence survival following multivariate analysis. Taking distant metastasis without liver involvement as a reference, liver metastasis was associated significantly with shorter OS at a hazard ratio (HR) of 1.581 (P = .021) and CSS at a HR of 1.643 (P = .016). Older age, keratinizing squamous cell carcinoma, no chemotherapy, and no radiotherapy were also prognosticators for poor OS (P

BACKGROUND: Papillary thyroid cancer (PTC) is the most common pathological type of thyroid cancer and its incidence is still on the rise worldwide. Our study aimed to provide an understanding of papillary thyroid cancer in immunological aspects and prompt its clinical diagnosis and treatment.METHODS: We obtained RNA-FPKM data and clinical data of papillary thyroid cancer (PTC) samples from the TCGA data portal. Immune-related genes (IRGs) were selected from Molecular Signatures Database v7.0. The immune-related lncRNAs were identified by constructing DELs-IRGs co-expression networks. Cox regression and Kaplan-Meier methods were used to construct immune-related lncRNAs prognosis index (IRLPI) and analyze the impact of IRLPI on overall survival. Gene Set Enrichment Analysis (GSEA) was performed by clusterProfiler R package to explore the potential molecular mechanism in TCGA dataset. The relationship between immune cell types and IRLPI were explored by using Pearson correlation analysis.RESULTS: A total of 478 primary PTC samples were collected for the analysis. 162 immune-related lncRNAs were identified in immune-lncRNAs co-expression network. And 7 lncRNAs were extracted to develop the IRLPI. Patients with high-IRLPI showed poor survival probability (P =0.00052) and IRLPI was identified as an independent prognostic factor (HR: 2.329, 95%CI: 1.481-2.664, p =2.54e-4). ROC curve declared a promising prognosis ability of IRLPI. PCA proved that IRLPI reflected different immune environment and biological processes. GSEA indicated that immune system development is the main different biological process between high- and low-IRLPI group. Furthermore, IRLPI was proved that it related with multiple immune cells and immune responses by Pearson correlation analysis.CONCLUSION: Our results showed that immune-related lncRNAs prognosis index (IRLPI) could serve as a potential prognostic model in papillary thyroid cancer and play an important role in the immunotherapies and surveillance of PTC.

A dye-sensitive photocatalytic H

To investigate the prevelence of early childhood caries (ECC) and its related risk factors in children aged 3 to 5 years old in Shanghai.According to the requirements of the Fourth National Epidemiological Survey of Oral Health, multistage, stratified, equal capacity and simple random sampling method was performed. A total of 1 296 children aged 3, 4 and 5 years old in 12 kindergartens in Shanghai were selected to calculate the prevalence and the mean dmft(decayed-missing-filled tooth). Their parents were surveyed with questionnaire on oral hygiene habit and consciousness of oral health and related factors. Statistical analysis were performed using SPSS 21.0 software package.The prevalence of ECC were 58.07% in children aged 3 to 5 years old in Shanghai, with dmft index of 2.99 in average. There were significant differences in the mean dmft and the prevalence between male and female and among different age groups(P0.05). In multivariate logistic regression analysis, more frequent sweet drinks, eating sweets before sleep, less daily tooth brushing, starting tooth brushing after 2 years old and low level of parents' education and oral health knowledge were risk factors for dental caries.The prevalence of ECC in children aged 3 to 5 years old in Shanghai is severe. There is an urgent need for implementation of a systematic education on diet habit and oral health behavior, and the parental education on oral hygiene needs to be further improved.

Scutellariae Radix(Huangqin) is a well-known traditional Chinese medicine(TCM) used for the treatment of clearing heat in clinical application. It is bitter-cold by using directly, but the bitter-cold property can be relieved after wine-frying. The study of taste changes before and after wine-frying of Scutellariae Radix is of great significance in identifying Scutellariae Radix and wine-processed Scutellariae Radix and clarifying the traditional theory of wine-processing. In this experiment, 10 batches of Scutellariae Radix and wine-processed Scutellariae Radix were prepared. The contents of 5 flavonoids were determined by high performance liquid chromatography(HPLC), and principal component analysis(PCA) was performed with 5 flavonoids as variables. As a result, the contents were different in different batches of Scutellariae Radix, but Scutellariae Radix and wine-processed Scutellariae Radix could not be distinguished. Five sensory attributes(sour, salty, fresh, sweet, and bitter) were evaluated by artificial tasting, and the response values of 7 sensors(AHS, AHS, PKS, CTS, NMS, CPS, ANS, SCS) representing the taste of pieces were detected by electronic tongue. The correlation between sensory evaluation and response values of the electronic tongue were analyzed, and the results showed that the sensory evaluation of sour, salty, fresh, sweet, bitter and AHS, CTS, NMS, ANS, SCS sensors had different degrees of correlation, indicating that the electronic tongue technology can be used as an alternative to artificial taste and can serve as a means for quantifying the taste, and it can be used to evaluate the taste of TCM pieces. The taste method was used to analyze the response values of the electronic tongue, and the results showed that the bitterness of wine-processed Scutellariae Radix decreased and the salty taste increased. PCA was used to analyze taste changes before and after wine-processed Scutellariae Radix, and the results showed that taste differences between 2 pieces were divided into 2 categories. PCA loading scattering plots showed that response of saltiness and bitterness were the major factors to affect overall taste in Scutellariae Radix and wine-processed Scutellariae Radix. Based on electronic tongue response values, the Fisher discriminant model for Scutellariae Radix and wine-processed Scutellariae Radix was established, which showed that it could effectively discriminate them with a recognition rate of 100%. The experimental results showed that the electronic tongue combined with multivariate statistical analysis can be used to evaluate taste of TCM, at the same time, it could provide a fast and simple method for identifying different processed products.

To investigate the expression level of miR-181b in CD19+ B lymphocytes of patients with chronic lymphocytic leukemia (CLL), to analyze the relationship between its expression and the prognosis of CLL patients, and to predict the potential target gene of miR-181b in CLL by using bioinformatics.Eight-four patients with CLL treated in People's Hospital of Xinjiang Uygur Autonomous Region from June 2013 to June 2018 were selected. and 20 healthy people were selected as control group. RNA was extracted from CD19+B lymphocytes of peripheral blood by magnetic bead sorting, the expression level of miR-181b was detected, and it's expression differences in different IPI groups were analyzed. The correlation between the expression level of miR-181b and PFS of CLL patients also was analyzed. miR-181b target genes were predicted by online database and literatures, and gene annotation analysis and relevant signal pathway analysis were performed for candidate target genes.The expression level of miR-181b in CLL patients was significantly lower than that in control group (P＜0.01); The expression level of miR-181b in the low-risk group was higher than that in high-risk group and extremely high-risk group (P＜0.05), but there was no statistical difference between low-risk group and medium-risk group (P=1.00). The expression level of miR-181b in medium-risk group was higher than that in high-risk group and extremely high-risk group (P＜0.05), but there was no difference between high-risk group and extremely high-risk group (P=1.00). ROC curve results showed that the area under the curve (AUC) was 0.792 (P＜0.01).When the expression level of miR-181b was at the threshold value of 0.279, it showed a better sensitivity (62.9%) and specificity (91.8%). Survival analysis results suggested that compared with the high expression group, the miR-181b low expression group had poor PFS (log rank: P=0.047). Prediction of miR-181b by using the starBase, targetscan and picTar database and its combination with literature reports indicated that CARD11, ZFP36L1, RUNX1, NR4A3, ATP1B1, PUM1 and PLAG1 related with blood diseases, and up-regulated CARD11 and ZFP36L1 participated in lymphoid tumor formation by promoting cell proliferation and inhibiting cell aging.The expression level of miR-181b in CLL group are significantly lower than that in the controls group, and the low expression of miR-181b relates with poor prognosis of CLL patients. Through bioinformatics prediction and combined with literature reports, it is speculated that CARD11 and ZFP36L1 as target genes of miR-181b may be participated in the occurrence and development of CLL. Further experiments are needed to verify this result.miR-181b在慢性淋巴细胞白血病患者中的表达及其靶基因的预测.探讨miR-181b在慢性淋巴细胞白血病（CLL）患者CD19+B淋巴细胞中的表达水平，分析其表达与CLL预后关系，并利用生物信息学预测miR-181b在CLL中的潜在靶基因.选取2013年6月至2018年6月在新疆自治区人民医院诊治的CLL患者84例、并选取健康者20例为对照组。应用磁珠分选CLL患者和对照组外周血CD19+ B淋巴细胞后进行RNA提取，检测miR-181b的表达水平，分析其在不同IPI分组中表达差异，探讨miR-181b表达水平与CLL患者PFS相关性。使用网上数据库及文献预测miR-181b靶基因,对候选靶基因进行基因注释分析及相关信号通路分析.miR-181b在CLL患者中表达显著低于对照组（P＜0.01）。miR-181b在低危组的表达水平高于高危组和极高危组（P＜0.05），但在低危组与中危组间表达无差异（P=1.00），中危组中miR-181b的表达水平高于高危组和极高危组（P＜0.05）,但在高危组与极高危组间表达无差异。ROC曲线结果显示，曲线下面积（AUC）为0.792（P＜0.01）， 由此显示，miR-181b表达水平处于分界值0.279时，具有较好的敏感性（62.9%）和特异性（91.8%）。生存分析结果提示，与高表达组相比，miR-181b低表达CLL患者具有较差的PFS（log-rank：P=0.047）。使用starBase、Targetscan和PicTar数据库进行靶基因预测，结合文献报道，与血液疾病相关的靶基因有CARD11、ZFP36L1、RUNX1、NR4A3、ATP1B1、PUM1和PLAG1，其中上调的CARD11、ZFP36L1通过促进细胞增殖、抑制细胞衰老参与淋巴系统肿瘤形成.miR-181b在CLL患者中的表达水平明显低于健康对照组，且miR-181b低表达与CLL不良预后有关。通过生物信息学预测并结合文献报道，推测CARD11及ZFP36L1可能作为miR-181b的靶基因参与CLL疾病发生发展，该结果还需要进一步实验进行验证.

Osteosarcoma is the most common primary malignant bone tumor in children and adolescents, with highly aggressive behavior and early systemic metastasis. The survival rates for osteosarcoma remain unchanged over the past two decades. Studies aiming to find new or alternative therapies for patients with refractory osteosarcoma are urgently needed. Anlotinib, a novel multi-targeted tyrosine kinase inhibitor (TKI), has exhibited encouraging clinical activity in NSLCC and soft tissue sarcoma, whereas its effect on osteosarcoma has not been studied. In our study, we investigated the anti-tumor activity and underlying mechanism of anlotinib in osteosarcoma. Various in vitro and in vivo models of human osteosarcoma were used to determine the anti-proliferative, anti-angiogenesis and anti-metastasis efficacy of anlotinib. Our results showed that anlotinib suppressed tumor growth and increased the chemo-sensitivity of osteosarcoma. In addition, anlotinib inhibited migration and invasion in osteosarcoma cells. Furthermore, in order to explore the anti-tumor mechanism of anlotinib, phospho-RTK antibody arrays were performed. These analyses confirmed that anlotinib suppressed the phosphorylation of MET, VEGFR2 and the downstream signaling pathway activation. Moreover, we demonstrated that anlotinib blocked hepatocyte growth factor (HGF)-induced cell migration, invasion and VEGF-induced angiogenesis. Notably, a 143B-Luc orthotopic osteosarcoma model further showed that anlotinib significantly inhibited growth and lung metastasis of implanted tumor cells. Our preclinical work indicates that anlotinib acts as a novel inhibitor of VEGFR2 and MET that blocks tumorigenesis in osteosarcoma, which could be translated into future clinical trials.

The formation of the microstructure in the rheo-diecastings were studied by the experiment of rheo-diecasting with semi-solid A380 Aluminum alloy slurry. The results show that the grain diameter and shape factor of primary α-Al grains decreased and increased along the rheo-diecasting direction, respectively, making the primary α-Al grains gradually distributing uniformly. The combination of injection pressure and narrow gate of the runner and ingate was the main reason for the evolution of the primary α-Al grains, moreover, the collision and friction among primary α-Al grains during the filling were helpful for the refinement and spheroidization of the primary α-Al grains. The residual liquid solidified into the secondary solidification microstructure accompanied by the evolution of the primary α-Al grains. The accumulation of the fragments of the primary α-Al grains, the secondary α2-Al nuclei or grains which formed in previous positions, the effective nucleation rate of the residual liquid, and the collision and fraction among the secondary α2-Al grains and between the secondary α2-Al grain and the primary α-Al grains made the secondary solidification microstructure of the residual liquid present multiplex and complicate characteristics. In this paper, the formation of the microstructure in the rheo-diecastings were studied by the experiment of rheo-diecasting. During the rheo-diecasting, the grain diameter and shape factor of primary α-Al grains decreased and increased, respectively along the rheo-diecasting direction, which made the primary α-Al grains gradually distribute uniformly. The drifting of the fragments of the primary α-Al grains and secondary α2-Al nucleus, and the effective nucleation rate of the residual liquid played an important effect on the morphology of the secondary solidification structure.

Research into catalytic materials that convert light energy into chemical energy by decomposing water still faces great difficulties. CuNi2S4 has attracted public attention due to its unique morphology; however, its high electron-hole recombination rate and weak charge separation efficiency limit its use in the field of catalysis. Herein, CoWO4 material is synthesized in situ on a CuNi2S4 surface by a hydrothermal method; the Z-type heterointerface constructed by the surface contact of the two materials under fusion shows a huge promotion effect on the catalytic ability. The performance (3754.3 μmol g-1 h-1) of the composite material (CW/CNS-12%) reached 42 and 6 times those of the two monomer materials, respectively, and the excellent catalytic ability of the composite is also reflected in its long-term stability. The experimental data proves that the modification of CuNi2S4 by building a heterostructure can improve the specific surface area of the material and the amount of carriers it generates. The charge transfer caused by the existence of the heterojunction hinders the recombination of carriers and holes; fluorescence and electrochemical characterization further confirms the charge transfer process in the Z-type heterojunction. This research provides new insights to promote catalytic performance by building heterostructures between monomer materials.

Esophageal squamous cell carcinoma (ESCC) occurs at a relatively high frequency in China and is one of the most prevalent cancers in the world. Genome-wide association studies (GWAS) have identified 24 single-nucleotide polymorphisms (SNPs) that could be associated with ESCC in Chinese patients. This retrospective study aimed to validate the association between these 24 SNPs and ESCC in a Han Chinese subgroup from East China. A total of 2280 and 1900 patients with ESCC (case group) and non-esophageal cancer (control group) were included from a single center. Genotyping of the 24 polymorphisms was performed using the Sequenom MassARRAY system. Unconditional logistic regression analyses were conducted for every polymorphism. It was found that rs12188136 (P=0.027, OR=1.158, 95% CI=1.016-1.319 for AG/AA) was associated with ESCC. Binary logistic regression analyses revealed a significant negative association of rs875339 in RORA (P=0.014, OR=0.762, 95% CI=0.613-0.947 for TT/CC). Under the dominant model, rs6854472 was slightly associated with ESCC risk (P=0.048, OR=1.192, 95% CI=1.002-1.418). Under the recessive model, a significant negative association was observed for rs875339 (P=0.010, OR=0.758, 95% CI=0.615-0.935). In a word, this large-scale replication study validated that rs12188136 and rs6854472 are associated with ESCC in a Han Chinese subgroup from Eastern China, and that rs875339 is negative associated with ESCC.

Objective: To develop a nomogram to predict the probability of live birth on the basis of the association of patient characteristics in subfertile individuals or couples. Methods: A retrospective study was conducted from January 2014 to December 2015. A nomogram was built from a training cohort and tested on an independent validation cohort. A total of 2,257 patients who had undergone their first nondonor cycle of in vitro fertilization (IVF) (including intracytoplasmic sperm injection) were randomly split 2:1 into training (n = 1,527) and validation (n = 730) cohorts. Results: There were no statistically significant differences in the patients' baseline and cycle characteristics between the training and validation cohorts. On multiple logistic regression analysis, female age, antral follicle count, tubal factor, anovulation, ethnicity, unexplained fertility, and male factor were significantly associated with live birth. The nomogram had a C-index of 0.700 (95% confidence interval [CI]: 0.698–0.701) in the training cohort and 0.684 (95% CI: 0.681–0.687) in the validation cohort. Conclusions: Our nomogram can predict the probability of live birth for infertile women and can be used to guide clinicians and couples to decide on an IVF treatment option.