251. A putative new order of methanogenic Archaea inhabiting the human gut, as revealed by molecular analyses of the mcrA gene
- Author
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Brugere, Jean-François, Mihajlovski, Agnès, ALRIC, Monique, Brugère, Jean-François, Conception, Ingénierie et Développement de l'Aliment et du Médicament (CIDAM), and Université d'Auvergne - Clermont-Ferrand I (UdA)
- Subjects
Male ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,Methanobacteriales ,[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] ,Gut flora ,[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy ,Feces ,RNA, Ribosomal, 16S ,[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,Phylogeny ,ComputingMilieux_MISCELLANEOUS ,Phylotype ,Genetics ,0303 health sciences ,biology ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,Methanobrevibacter smithii ,General Medicine ,Middle Aged ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] ,DNA, Archaeal ,Female ,Oxidoreductases ,Methane ,Adult ,animal structures ,Methanogenesis ,Archaeal Proteins ,Molecular Sequence Data ,Microbiology ,DNA, Ribosomal ,03 medical and health sciences ,[SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems ,[SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system ,Phylogenetics ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Humans ,Molecular Biology ,030304 developmental biology ,Aged ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,030306 microbiology ,[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Ribosomal RNA ,biology.organism_classification ,Archaea ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Gastrointestinal Tract ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,[SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis - Abstract
The diversity of methanogenic Archaea from the gut of 6 humans was investigated by targeting mcrA, a molecular metabolic marker of methanogenesis. Three operational taxonomic units (OTUs) were recovered from about 400 clones analyzed, two of which were attributed to the expected Methanobacteriales Methanobrevibacter smithii (4 volunteers) and Methanosphaera stadtmanae (1 volunteer). The third OTU (1 volunteer) corresponded to a distant phylotype that does not cluster with any of the five methanogenic orders. This result, also supported by 16S archaeal sequences retrieved from the same volunteer, strongly suggests there may be a sixth order and hence potential underestimation of the role of methanogens in gut physiology.
- Published
- 2008
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