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Eleven species of anamorphic fungi associated with anthracnose and fruit rot symptoms of Momordica cochinchinensis (Lour.) Spreng. (Ban Kakrol) have been described. The associated fungi were Aspergillus niger Van Tieghem, Colletotrichum gloeosporioides (Penz.) Sacc., C. orbiculare (Berk. & Mont.) Arx., Corynespora cassiicola (Berk. & Curt.) Wei, Curvularia clavata Jain, Dendryphiella vinosa (Berk. & Curt.) Reisinger, Fusarium moniliforme J. Sheld., Lasidiodiplodia theobromae (Pat.) Griff. & Maubl., Pestalotiopsis guepinii (Desm.) Stey., Penicillium digitatum Sacc. and Xylohypha pinicola D. Hawksw. Xylohypha pinicola is a new record for Bangladesh. [ABSTRACT FROM AUTHOR]

Two donor-π-spacer-acceptor (D-π-A) organic dyes were designed as photochromic dyes with the same π-spacer and acceptor but different donors, based on their electron-donating strength. Various structural, electronic, and optical properties, chemical reactivity parameters, and certain crucial factors that affect short-circuit current density (Jsc) and open circuit voltage (Voc) were investigated computationally using density functional theory and time-dependent density functional theory. The trans-cis isomerization of these azobenzene-based dyes and its effect on their properties was studied in detail. Furthermore, the dye-(TiO2)9 anatase nanoparticle system was simulated to understand the electronic structure of the interface. Based on the results, we justified how the trans-cis isomerization and different donor groups influence the physical properties as well as the photovoltaic performance of the resultant dye-sensitized solar cells (DSSCs). These theoretical calculations can be used for the rapid screening of promising dyes and their optimization for photochromic DSSCs.

A series of donor–π-conjugated spacer–acceptor (D–π–A) organic dyes featuring an azobenzene spacer were designed as chromic dyes and investigated computationally. The electron-donating strength was modified by introducing electron-donating units to the donor side. In particular, the trans–cis isomerization of the azobenzene-based dyes and its effect on the optical and electronic properties were further scrutinized. In both trans and cis conformers, a gradual increase in electron-donating strength promoted the natural charge separation between donor and acceptor moieties, thereby allowing the absorption of a longer wavelength of visible light. Importantly, the conformational change of the azobenzene bridge resulted in different absorption spectra and light-harvesting properties. The azobenzene-based dyes will open up a new research path for chromic dye-sensitized solar cells.

BackgroundDhaka City, the capital of Bangladesh, has experienced rapid and unplanned urbanization over the past few decades. This process has brought significant challenges to public health as the urban environment has become a breeding ground for various health risks. Understanding the associations between unplanned urbanization, the urban environment, and public health in Dhaka City is crucial for developing effective interventions and policies.ObjectivesThis review paper aims to uncover the associations between unplanned urbanization and health risks in Dhaka City, with a specific focus on the urban environment and its impact on public health. The objectives of this study are to examine the health challenges faced by the city’s population, explore the specific urban environmental factors contributing to health risks, analyze the socioeconomic determinants of health in unplanned urban areas, evaluate existing policies and governance structures, identify research and data gaps, and provide recommendations for future interventions.MethodsA comprehensive literature review was conducted to gather relevant studies, articles, reports, and policy documents related to unplanned urbanization, the urban environment, and public health in Dhaka City. Various databases and online resources were searched, and the selected literature was critically analyzed to extract key findings and insights.ResultsThe findings reveal that unplanned urbanization in Dhaka City has led to a range of public health risks, including air pollution, inadequate water and sanitation, poor waste management, overcrowding, slums, and substandard housing conditions. These environmental factors are strongly associated with respiratory diseases, waterborne illnesses, and other adverse health outcomes. Socioeconomic determinants such as poverty, income inequality, and limited access to healthcare further exacerbate the health risks faced by the urban population.ConclusionUnplanned urbanization in Dhaka City has significant implications for public health. Addressing the associations between unplanned urbanization, the urban environment, and public health requires comprehensive policies and interventions. Improved urban planning, enhanced infrastructure, and better policy governance are essential for mitigating health risks. Furthermore, addressing socioeconomic disparities and ensuring equitable access to healthcare services are crucial components of effective interventions.

The malignant neoplastic malady known as cancer appears to exhibit a significantly elevated rate of mortality owing to its virulence and pronounced propensity for metastasis. To augment the diagnostic efficacy, research endeavors have been undertaken utilizing complex deep learning architectures. However, the performance of these efforts remains circumscribed by smaller dataset size, quality of the data, the interclass variations present between lung adenocarcinoma and lung squamous cell carcinoma, and the complexity of deploying to mobile devices and failure to address both image and patient level accuracy measurements. To surmount these obstacles, the present study proposes a stage-based method for enhancing the images, in conjunction with utilizing a global context attention-guided convolutional neural network that effectively captures both channel and spatial information and semantic information extracted from the input image. Implementing the proposed methodology increased total image level accuracy to 99.76% and a patient level accuracy of 96.5%, a metric that has yet to be previously quantified. The addition of the global context attention module decreases the model’s parameter count by 0.47 million, reduces the computational costs by saving 10.54 million floating point operations per second (FLOPs) and 10.72 million multiply-accumulate operations (MACs), and results in a 0.03s improvement in inference time. Furthermore, this module enhances both image level and patient level accuracy, boosting them by 2.84% and 3.17%, respectively, compared to using only the convolutional block attention module in the baseline convolutional neural network. Consequently, this modification renders the model highly suitable for deployment on mobile devices due to its adaptability. Our findings provide supporting evidence for the potential of this method to serve as a noninvasive screening tool capable of reliably classifying lung and colon cancer subtypes.

Information on molecular genetic diversity among Hibiscus cannabinus L. lines is crucial for developing a new variety from the existing germplasms based on consumer demand. Based on this premise, the allelic diversity of thirty-one kenaf genotypes were assessed using polymorphic EST-SSR. From the 72 EST-SSR primers tested, 10 showed sharp and clear polymorphic bands ranging from 4 (137 to 225 bp) to 7 (182 to 382 bp) alleles with a mean value of 5.2 per primer. The Polymorphism Information Content (PIC) value ranged from 0.531 to 0.737, with a mean of 0.610, indicating that the genotypes are genetically diverse. Shannon index estimation varied from 0.982 to 1.515, whereas 0.123 to 0.405 was recorded within the groups. Genetic differentiation ranged from 0.67 to 1 alongside the average gene flow (Nm) of 0.024. The AMOVA revealed 76% variation within the population, while the population gain recorded was 24%. UPGMA dendrogram indicated the relationship and categorized the evaluated kenaf mutants into five major groups. This study revealed the existence of significant variation in kenaf mutants based on EST-microsatellites. These results could be used for future breeding programs and conservation of germplasms to enhance Hibiscus cannabinus L. genotypes.

Abstract Background World Health Organization (WHO) has classified drowning as a major public health problem. The most vulnerable victims of drowning are children from low and middle‐income countries. Previously, it was the primary cause of death among children aged between 1 and 17 years in Bangladesh. Aims This study explored the surrounding circumstances and associated factors of child drownings in Bangladesh. Methods A qualitative phenomenological approach has been used to conduct the study. Bangladesh was chosen as the study area, and data were gathered using a semi‐structured, open‐ended questionnaire. Using convenience and snowball sampling methods, we have collected data from Dhaka and seven additional districts in Bangladesh. We reached a total of 44 individuals, where 22 agreed to participate in an interview (face‐to‐face and online interviews). The remaining 22 participants were selected in two focus group discussions via the web platform “ZOOM cloud meeting.” Results Our investigation revealed several factors associated with child drowning, including a lack of adequate parental supervision and monitoring, geographic locations and environment, seasonal factors, low living standards, peer pressure and risky behaviors, social stigma and prejudices, and natural disasters and calamities. According to our findings, a lower socioeconomic position is linked to a higher risk of nonfatal drowning. Moreover, this research also indicates a substantial nexus between child drowning fatalities and the socioeconomic conditions of the families of the victims. Conclusion The study adds to the existing body of knowledge by underlining the associated factors of child drowning fatalities in Bangladesh, which will aid in developing preventive policies. An essential aspect of any drowning prevention program for Bangladesh should be enhanced for community awareness of safe water rescue and resuscitation practices.

Background A previous study identified students taking Bangladeshi university entrance tests as a vulnerable group at a higher risk of suicidal behaviours before the COVID-19 pandemic. However, the impact of the pandemic on the magnitude of these behaviours among this population remains unexplored. Aims This study aimed to investigate the prevalence of suicidal ideation and associated factors among Bangladeshi university entrance test takers following the pandemic. In addition, an approach based on Geographic Information System (GIS) data was used to visualise the distribution of suicidal ideation across the country. Methods A cross-sectional approach was used to collect data among participants taking the entrance test at Jahangirnagar University in September 2022. Using SPSS, data were analysed with chi-squared tests and binary regression, and ArcGIS was used to map the results across the nation. Results The study revealed a prevalence of 14.4% for past-year suicidal ideation, with 7.4% and 7.2% reporting suicide plans and attempts, respectively. Notably, repeat test-takers exhibited a higher prevalence of suicidal behaviours. Significant risk factors for suicidal ideation included urban residence, smoking, drug use, COVID-19 infection and deaths among close relations, depression, anxiety and burnout. The GIS-based distribution indicated significant variation in the prevalence of suicidal ideation across different districts, with higher rates observed in economically and infrastructurally deprived areas. Conclusions Urgent measures are needed to address the high prevalence of suicidal behaviours among students taking university entrance tests students in Bangladesh, particularly in light of the COVID-19 pandemic. Enhanced mental health support, targeted prevention efforts and improved resources in economically disadvantaged regions are crucial to safeguard the well-being of these students.

Abstract Nine morphologically distinct kenaf genotypes were hybridized to produce 36 hybrids following a half diallel mating design. The combining ability and gene action of 15 yield and yield components were assessed in hybrids and their parents across two environments. Except for the mid diameter and plant height traits, there were highly significant differences (p ≤ 0.01) between the environments and the interaction of genotype and environment. Additive gene effects were considerable for the inheritance of these traits, and the expression of these additive genes was heavily influenced by the environment. Significant differences were found for all studied traits for GCA except top diameter and SCA except plant height and top diameter, implying the presence of both additive and non-additive gene action for the inheritance of the concerned characters. For all features except top diameter and number of nodes, the magnitude of GCA variation was significantly higher than that of SCA variance, indicating the additive gene's predominance. The parental lines P1, P3 and P4 were outstanding general combiners for fiber yield and yield-related parameters. Considering combining ability and genetic analysis study, the crosses P1 × P4, P1 × P9, P2 × P3, P2 × P5, P4 × P6, P4 × P7, P4 × P9, P5 × P8, and P7 × P9 were found promising for their heterotic response to higher fiber yield, stick yield, seed yield and could be for future improvement in kenaf breeding programmes.

Kenaf is a fiber crop belonging to the genus Hibiscus (Malvaceae), and the potential of this plant, its multipurpose use, and its environmentally friendly cellulose have placed kenaf at the forefront of both commercial and subsistence farming. Due to inadequate agricultural practices, low production potential, and photoperiodism, the yields of fiber are relatively low. The varietal development in kenaf is hindered by a lack of knowledge of its heredity, which also affects its successful usage and protection of important germplasm. The amount and production potential of kenaf are enhanced by identifying and exploring obtainable genetic resources for the development of high-yield cultivars. Knowledge of existing natural genetic variation and its availability is important for this highly valued crop’s genetic improvement. This review summarized recent advances in kenaf varietal improvement using marker-assisted breeding, genetic linkage maps, and morphological and molecular assessment of genetic diversity. Existing issues were discussed to produce scientific references for identifying photo-insensitive kenaf genotypes.

Dhaka city is experiencing rapid land cover changes, and the effects of climate change are highly visible. Investigating their combined influence on runoff patterns is vital for sustainable urban planning and water resources management. In this work, multi-date land cover classification was performed using a random forest (RF) algorithm. To validate accuracy of land cover classification, an assessment was conducted by employing kappa coefficient, which ranged from 85 to 96%, indicating a high agreement between classified images and the reference dataset. Future land cover changes were forecasted with cellular automata-artificial neural network (CA-ANN) model. Further, soil conservation service -curve number (SCS-CN) rainfall-runoff model combined with CMIP6 climate data was employed to assess how changes in land cover impact runoff within Dhaka metropolitan development plan (DMDP) area. Over the study period (2020–2100), substantial transformations of land cover were observed, i.e., built-up areas expanded to 1146.47 km2 at the end of 2100, while agricultural areas and bare land diminished considerably. Consequently, monsoon runoff increased from 350.14 to 368.24 mm, indicating elevated hydrological responses. These findings emphasized an intricate interplay between urban dynamics and climatic shifts in shaping runoff patterns, underscoring urgency of incorporating these factors into urban planning strategies for sustainable water resources management in a rapidly growing city such as Dhaka.

In the current study, nanocrystalline CoY0.5xLa0.5xFe2-xO4 (where x = 0.00, 0.02, 0.04, 0.06, 0.08, and 0.10) ferrites have been synthesized via a sol-gel auto combustion process. The synthesized powders were pressed into pellet forms and sintered at 900 °C for 4 h in the air. X-ray diffractometry (XRD) confirmed the single-phase cubic spinel structure of the synthesized samples having the mean crystallite domain sizes ranging from 122 and 54 nm. FTIR spectroscopic analyses revealed two strong bands within the range of 600 to 350 cm−1, further confirming the cubic inverse spinel structure of the prepared materials. The surface morphologies and composition were investigated by Field Emission Scanning Electron Microscopy (FE-SEM) and Energy Dispersive X-ray (EDX) Spectroscopy. The magnetic hysteresis curves recorded at room temperature exhibit ferrimagnetic behavior. The highest coercivity (Hc∼1276 Oe) was found at a high doping (x = 0.10) concentration of Y3+ and La3+ in cobalt ferrite. Dielectric constant increase with increased doping concentration whereas real-impedance and dielectric loss decrease with increased in doping concentration and applied frequency. The band gap energy increased from 1.48 to 1.53 eV with increasing Y3+ and La3+concentrations in the UV–Vis region. The elevated levels of magnetic and dielectric substances in the ferrite nanoparticles suggest that the material could be used for magnetic recording media and high-frequency devices.

BackgroundIn order to minimize the maternal and child mortality rate, the presence of skilled birth attendants (SBA) during delivery is essential. By 2022, 4th health, population and nutrition sector programme in Bangladesh aims to increase the percentage of deliveries performed by SBA to 65 percent. The objective of the present study was to determine the rate and associated factors of usage SBA among Bangladeshi mothers during their delivery.MethodsThis study utilized secondary data that was collected by Bangladesh Demographic and Health Survey (BDHS) 2017-18. The usage of SBA was measured by a question to respondent, who assisted during your delivery? It was classified into two classes; (i) skilled birth attendant (qualified doctors, nurses, midwives, or paramedics; family welfare visitors, community skilled birth attendants, and sub-assistant community medical officers) (code 1), and (ii) unskilled birth attendant (untrained traditional birth attendants, trained traditional birth attendants, relatives, friends, or others) (code 0). Two logistic regression model was used to determine the associated factors of SBA after removing the cluster effect of the outcome variable.ResultsThis study found 53.2% mothers were delivered by SBA in Bangladesh, among them 56.33% and 42.24% mothers were delivered by nurse/midwife/paramedic and doctor respectively. The two level logistic model demonstrated that geographical location (division), type of residence, religion, wealth index, mothers' body mass index, mothers' education level, mothers' occupation, total ever born children, mothers' age at first birth (year), number of ANC visits, husbands' education level and husbands' occupation were significant (pConclusionsThis study revealed that still 46.8% mothers are delivered by unskilled birth attendant, this might be treated of Bangladesh Government to achieve SDGs indicator 3.1.2 by 2030. Counseling could be integrated during ANC to increase awareness, and should ensure for every Bangladeshi mothers visit ANC service during their pregnancy at least 4 times.

Abstract Ligamentum flavum hypertrophy (HLF) is the most important component of lumbar spinal canal stenosis (LSCS). Analysis of hypertrophied ligamentum flavum (HLF) samples from patients with LSCS can be an important que. The current study analyzed the surgical samples of HLF samples in patients with LCSC using quantitative and qualitative high performance-liquid chromatography and mass spectrometry. We collected ligamentum flavum (LF) tissue from twelve patients with LSCS and from four patients with lumbar disk herniation (LDH). We defined LF from LSCS patients as HLF and that from LDH patients as non-hypertrophied ligamentum flavum (NHLF). Total lipids were extracted from the LF samples and evaluated for quantity and quality using liquid chromatography and mass spectrometry. The total lipid amount of the HLF group was 3.6 times higher than that of the NHLF group. Phosphatidylcholines (PCs), ceramides (Cers), O-acyl-ω-hydroxy fatty acids (OAHFAs), and triglycerides (TGs) in the HLF group were more than 32 times higher than those of the NHLF group. PC(26:0)+H+, PC(25:0)+H+, and PC(23:0)+H+ increased in all patients in the HLF group compared to the NHLF group. The thickness of the LF correlated significantly with PC(26:0)+H+ in HLF. We identified the enriched specific PCs, Cers, OAHFAs, and TGs in HLF.

Abstract Protonic ceramic fuel cells (PCFCs) have become the most efficient, clean and cost-effective electrochemical energy conversion devices in recent years. While significant progress has been made in developing proton conducting electrolyte materials, mechanical strength and durability still need to be improved for efficient applications. We report that adding 5 mol% Zn to the Y-doped barium cerate-zirconate perovskite electrolyte material can significantly improve the sintering properties, mechanical strength, durability and performance. Using same proton conducting material in anodes, electrolytes and cathodes to make a strong structural backbone shows clear advantages in mechanical strength over other arrangements with different materials. Rietveld analysis of the X-ray and neutron diffraction data of BaCe0.7Zr0.1Y0.15Zn0.05O3−δ (BCZYZn05) revealed a pure orthorhombic structure belonging to the Pbnm space group. Structural and electrochemical analyses indicate highly dense and high proton conductivity at intermediate temperature (400–700 °C). The anode-supported single cell, NiO-BCZYZn05|BCZYZn05|BSCF-BCZYZn05, demonstrates a peak power density of 872 mW cm−2 at 700 °C which is one of the highest power density in an all-protonic solid oxide fuel cell. This observation represents an important step towards commercially viable SOFC technology.

To understand the ultra-early reaction of normal organ lipids during irradiation, we investigated the response of lipids, including polyunsaturated fatty acid (PUFA) chains, which are particularly susceptible to damage by ROS, in mice’s kidneys, lungs, brains, and livers within 5 min of single high-dose irradiation. In this study, we set up three groups of C56BL/6 male mice and conducted whole-body irradiation with 0 Gy, 10 Gy, and 20 Gy single doses. Kidney, lung, brain, and liver tissues were collected within 5 min of irradiation. PUFA-targeted and whole lipidomic analyses were conducted using liquid chromatography–tandem mass spectrometry (LC-MS/MS). The results showed that PUFA chains of kidney phosphatidylcholine (PC), phosphatidylethanolamine (PE), and triacylglycerol (TG) significantly increased within 5 min of 10 Gy and 20 Gy irradiation. The main components of increased PUFA chains in PC and PE were C18:2, C20:4, and C22:6, and in TG the main component was C18:2. The kidney lipidomes also showed significant changes from the perspective of lipid species, mainly dominated by an increase in PC, PE, TG, and signal lipids, while lipidomes of the lung, brain, and liver were slightly changed. Our results revealed that acute PUFA chains increase and other lipidomic changes in the kidney upon whole-body irradiation within 5 min of irradiation. The significantly increased lipids also showed a consistent preference for possessing PUFA chains. The lipidomic changes varied from organ to organ, which indicates that the response upon irradiation within a short time is tissue-specific.

This Research was conducted to investigate the effect of fertilizer management on growth and yield performance of aromatic fine rice varieties. The experiment consisted of two factors were aromatic fine rice and fertilizer management. There were four varieties namely Kalizira, Kataribhog, Tulshimala and BRRI Dhan34 with four fertilizer treatments recommended dose of fertilizers (T1), cowdung @ 10 tha-1 (T2), 50% of recommended dose of fertilizers + 50% cowdung(T3), 75% of recommended dose of fertilizers + 50% cowdung (T4). The result showed that BRRI Dhan 34 significantly superior for effective tillers number/ hill (18.46), panicles length (26.67cm), grains number/ panicle (146.30), harvest index (40.73 %), grain protein content (6.23%), grain yield (2.79 tha-1), straw yield (4.06 tha-1), and biological yield (6.85 tha-1). Among fertilizer management, the highest effective tillers number/hill (16.20), grains number/ panicle (142.45, panicles length (26.66 cm),1000 grain weight (13.75), grain protein content (10.2%), biological yield (6.30), were obtained from T3 treatment. Based on interaction effect showed that the highest effective tillers number/hill (18.36), grains number/ panicle (155.33), panicles length (26.73 cm), grain protein content (10.80%), biological yield (7.85) was found with BRRI Dhan 34 combined with T3 treatment. However, grain yield increased with the increase in nitrogen levels. Together tillers number/hill, grains number/panicle, grain protein content, harvest index, grain yield was the main responsible yield contributing characters to improve the yield quality of aromatic fine rice.

Abstract Background Early onset of menarche is one of the most important factors for breast cancer and other associated health hazards. The aim of this study was to investigate the early age at menarche and its associated factors in school girls (age, 10–12 years) in Rajshahi District, Bangladesh. Methods Data was collected from Rajshahi District, Bangladesh, using multistage random sampling. Independent sample t test and binary logistic regression model were used in this study. A total number of 386 school girls aged 10–12 years were considered as a sample for this study. Results This study revealed that more than 48% girls already attained menarche within the age of 12 years, among them 25.6%, 41.0%, and 58.3% girls experienced menarche at the age of 10, 11, and 12 years, respectively. It was observed that the menarcheal girls were significantly taller (p < 0.01) and heavier (p < 0.01) than non-menarcheal girls. The menarcheal girls’ mothers were heavier (p < 0.01), shorter (p < 0.01), had more BMI (p < 0.01), reached menarche (p < 0.05) earlier than non-menarcheal girls’ mothers. Menarcheal girls had less number of siblings (p < 0.01) and lower order of birth (p < 0.05) than non-menarcheal girls. After controlling the effect of other factors, multiple logistic regression model demonstrated that obese girls were more likely to attain menarche than under- [AOR = 0.279, CI 95% 0.075–0.986; p < 0.05] and normal [AOR = 0.248, CI 95% 0.082–0.755; p < 0.05] weight girls. Urban school girls had more chance to get menarche than rural school girls at same age (AOR = 0.012, 95% CI 0.003–0.047; p < 0.01). Conclusions Therefore, modern lifestyle changes may have the important factors for early age at menarche of the studied girls in Bangladesh.

Spinel ferrite [Formula: see text][Formula: see text][Formula: see text]Fe2O4 was prepared by a conventional ceramic process followed by sintering at three different temperatures (1050[Formula: see text] C, 1100[Formula: see text] C and 1150[Formula: see text] C). X-ray diffraction (XRD) investigations stated the single-phase cubic spinel structure and the FTIR spectra revealed two prominent bands within the wavenumber region from 600 cm[Formula: see text] to 400 cm[Formula: see text]. Surface morphology showed highly crystalline grain development with sizes ranging from 0.27 [Formula: see text]m to 0.88 [Formula: see text]m. The magnetic hysteresis curve at ambient temperature revealed a significant effect of sintering temperature on both coercivity ([Formula: see text] and saturation magnetization ([Formula: see text]. Temperature caused a decrease in DC electrical resistivity, while the electron transport increased, suggesting the semiconducting nature of all samples and that they well followed the Arrhenius law from which their activation energies were determined. The values of Curie temperature ([Formula: see text] and activation energy were influenced by the sintering temperature. Frequency-dependent dielectric behavior (100 Hz–1 MHz) was also analyzed, which may be interpreted by the Maxwell–Wagner-type polarization. The UV–vis–NIR reflectance curve was analyzed to calculate the bandgap of ferrites, which showed a decreasing trend with increasing sintering temperature.

Polycrystalline NiCuZn ferrite (NixCu0.3Zn0.7−xFe2O4; x=0.2, 0.3, 0.4 and 0.5) were prepared through sol–gel auto combustion method applying double sintering technique. Structural, morphological, elemental analyses (EDS), Fourier-transform infrared spectroscopy (FTIR), Direct Current (DC) electrical resistivity, dielectric, magnetic and optical properties of prepared samples were analyzed. XRD profiles reveal the formation of simple cubic spinel structure without any traceable impurity. The average crystallite size lies within the range of 22–29nm. Lattice parameter decreases with increasing Ni concentration. Room temperature DC resistivity was recorded from 6.39×105 to 3.79×105Ωcm. Both dielectric constant (ε̇) and loss factor (tanδ) were decreased with increase of frequency while AC conductivity increases. FTIR absorption peak occurred at three different frequency ranges at 570–577cm−1, 1635–1662cm−1 and 3439–3448cm−1. Magnetic properties were investigated by using vibrating sample magnetometer (VSM). Decreasing trends were observed for saturation magnetization (Ms), magnetic coercivity (Hc) and remanant magnetization (Mr) with the increase of Ni content. Optical band gap (∼2.70–2.79eV) were calculated from diffuse reflectance data by using Kubelka–Munk function.

Proline metabolism influences the metabolic and/or signaling pathway in regulating plant stress responses. This study aimed to characterize the physiological significance of glutamate (Glu)-mediated proline metabolism in the drought stress responses, focusing on the hormonal regulatory pathway. The responses of cytosolic Ca2+ signaling, proline metabolism, and redox components to the exogenous application of Glu in well-watered or drought-stressed plants were interpreted in relation to endogenous hormone status and their signaling genes. Drought-enhanced level of abscisic acid (ABA) was concomitant with the accumulation of ROS and proline, as well as loss of reducing potential, which was assessed by measuring NAD(P)H/NAD(P)+ and GSH/GSSG ratios. Glu application to drought-stressed plants increased both salicylic acid (SA) and cytosolic Ca2+ levels, with the highest expression of calcium-dependent protein kinase (CPK5) and salicylic acid synthesis-related ICS1. The SA-enhanced CPK5 expression was closely associated with further enhancement of proline synthesis-related genes (P5CS1, P5CS2, and P5CR) expression and a reset of reducing potential with enhanced expression of redox regulating genes (TRXh5 and GRXC9) in a SA-mediated NPR1- and/or PR1-dependent manner. These results clearly indicate that Glu-activated interplay between SA- and CPK5-signaling as well as Glu-enhanced proline synthesis are crucial in the amelioration of drought stress in Brassica napus.

Dementia is a major public health concern nowadays. Reduced levels of brain docosahexaenoic acid (DHA) and DHA-phosphatidylcholines (DHA-PCs) in dementia patients were reported previously. Recently, we have reported that supplementation of green nut oil (GNO) or DHA improves memory function and distribution levels of brain DHA in senescence accelerated mice P8 (SAMP8). GNO is extracted from Plukenetia volubilis seeds, and SAMP8 is a well-known model mouse of dementia. In this current study, we examined the results of GNO or DHA supplementation in the distribution levels of brain DHA-PCs in same model mouse of dementia using desorption electrospray ionization (DESI) mass spectrometry imaging (MSI). We observed significantly decreased distribution of brain DHA-PCs, PC (16:0_22:6), and PC (18:0_22:6) in SAMP8 mice compared to wild type mice, and GNO or DHA treatment restored the decreased distribution levels of PC (16:0_22:6) and PC (18:0_22:6) in the brain of SAMP8 mice. These results indicate that GNO or DHA supplementation can ameliorate the decreased distribution of brain DHA-PCs in dementia, and could be potentially used for the prevention and treatment of dementia.

The vermilion of the human lip is a unique facial area because of certain distinguishing features from the adjacent tissues such as the white lip (skin) and oral mucosa. However, the distinction in terms of molecular distribution between the vermilion and skin has remained unexplored. Therefore, we aimed to map the human lip by mass spectrometry imaging to gain understanding of the free fatty acid distribution in the vermilion. The lip specimens trimmed off during cheiloplasty were analyzed using desorption electrospray ionization–mass spectrometry imaging. Distributions of two monounsaturated fatty acids and three polyunsaturated fatty acids were observed in the human lip tissue: palmitoleic acid (POA) and oleic acid (OA) and linoleic acid (LA), arachidonic acid (AA), and docosahexaenoic acid (DHA), respectively. Although POA, OA, LA, and AA were differentially distributed across the vermilion and skin, DHA showed a higher accumulation in the epithelium of the vermilion compared to that in the skin. Our results clearly demonstrated the difference in fatty acid distributions between the vermilion and skin. The highly abundant DHA in the epithelium of the vermilion may have an antioxidant role and may thus protect the lip from aging. Our findings can provide a novel strategy for treating lip disorders.

To characterize cultivar variation in resistance gene (R-gene)-mediated calcium signaling and hormonal regulation in effector-triggered immunity (ETI) and disease susceptibility, Xanthomonas campestris pv. campestris (Xcc) was inoculated in two Brassica napus cultivars (cvs. Capitol and Mosa). At 14 days post inoculation (DPI) with Xcc, there was a necrotic lesion in cv. Mosa along with the significant accumulation of H2O2 and malondialdehyde (MDA), whereas no visual symptom was observed in cv. Capitol. The cultivar variations in the R-gene expressions were found in response to Xcc. ZAR1 is a coiled-coil-nucleotide binding site-leucine-rich repeat (CC-NB-LRR)-type R-gene that is significantly induced in cv. Capitol, whereas toll/interleukin-1 receptor-nucleotide binding site-leucine-rich repeat (TIR-NB-LRR)-type R-gene, TAO1, is significantly upregulated in cv. Mosa Xcc-inoculated plants. The defense-related gene’s non-race-specific disease resistance 1 (NDR1) and mitogen-activated protein kinase 6 (MAPK6) were enhanced, whereas calcium-dependent protein kinase (CDPK5) and calcium-sensing protein 60g (CBP60g) were depressed in cv. Capitol Xcc inoculated plants, and opposite results were found in cv. Mosa. The calcium-sensing receptor (CAS), calmodulin (CaM), expression was induced in both the cultivars. However, the CAS induction rate was much higher in cv. Mosa than in cv. Capitol in response to Xcc. The phytohormone salicylic acid (SA) and jasmonic acid (JA) levels were significantly higher in cv. Capitol along with the enhanced SA receptors (NPR3 and NPR4) and JA synthesis and signaling-related gene expression (LOX2, PDF1.2), whereas the JA level was significantly lower in cv. Mosa Xcc inoculated plants. The SA synthesis and signaling-related genes (ICS1, NPR1) and SA were present at higher levels in cv. Mosa; additionally, the SA level present was much higher in the susceptible cultivar (cv. Mosa) than in the resistant cultivar (cv. Capitol) in response to Xcc. These results indicate that ZAR1 mediated the coordinated action of SA and JA synthesis and signaling to confirm ETI, whereas TAO1 enhanced the synthesis of SA through CAS and CBP60g to antagonize JA synthesis and signaling to cause disease susceptibility in the Brassica napus−Xcc pathosystem.

Zinc substituted cobalt ferrite nanoparticles with elemental composition Co1−xZnxFe2O4 (x=0.0, 0.2, 0.4, 0.6) were prepared by the sol-gel auto-combustion technique using Co, Fe, Zn nitrate as a precursor where nitrates to citrate was 1:3. The as prepared powder of cobalt zinc ferrite was sintered at 900∘C for 3h. Structural, morphological, dielectric and magnetic properties were studied by x-ray diffractometer (XRD), scanning electron microscope (SEM), high precision impedance analyzer and vibrating sample magnetometer (VSM), respectively. The peaks obtained from the XRD confirmed samples having crystallite (∼32–36nm) single phase inverse spinel structure without any traceable impurity. Lattice parameters were calculated from XRD and it increases with Zn content. SEM revealed irregularly shaped grains (∼0.5–0.7μm) morphology with heterogeneous distribution. The dielectric constant (ε′) and dielectric loss (tanδ), have been measured as a function of frequency at room temperature. The dependence of ε′ and tanδ with frequency showed the normal dielectric behavior in accordance with the Maxwell-Wagner type of interfacial polarization and electron hopping change between Co2+ and Co3+ as well as between Fe2+ and Fe3+ ions at octahedral sites.

The scarcity of hygienic drinking water is a normal phenomenon in the coastal areas of Bangladesh due to the high salinity of ground water. The inhabitants of this locality, therefore, live on alternative supplies of water including rain-fed pond water, and rainwater with persistent complex microbial interactions therein, often contaminated with life-threatening pathogens. Hence, this study was aimed at analyzing the prevalence of Vibrio cholerae (Vc) in the alternative drinking waters of Mathbaria, a coastal subdistrict neighboring the Bay of Bengal, the efficacy of pond sand filter (PSF) and the co-association among Bacillus-like spore formers (Sf) and Vc. Vc presumably entrapped into the membrane filter was enriched in alkaline peptone water medium and was isolated on selective thiosulfate-citrate-bile salts-sucrose and taurocholate-tellurite-gelatin agar media. They were finally identified by immunochromatographic one step rapid test and serology test. A total of 26% Vc positive samples were obtained out of 100 [ponds—48, household (HH)—29, and PSFs—23] where 13% cases were pathogenic (Vc O1) and 13% were non-pathogenic (Vc non-O1/non-O139). The distribution of Vc as observed was 33, 26, and 13.8% in waters derived from pond surface, PSF, and HH reservoirs, respectively, and for pathogenic type, it was 62.5%, 50%, and nil, respectively. Although none of the samples was identified with pathogenic Vc O139, the statistics represents a significant and augmentative risk of cholera outbreak in the focused area. The antibiotic sensitivity pattern in this study resembled the trend observed during last few years for Vc. The PSF demonstrated its inability to remove Vc from any of the samples and in addition, the filter itself was evidenced to be the source of pathogens and spores in further contamination and transmission. The development of biofilm in the PSF could be hypothesized as the reservoir in contaminating pathogen-free water samples. From the test of homogeneity, the risk levels of alternative water sources were estimated equal regarding Vc. Simultaneously, it was determined statistically that the prevalence of Vc, by no means, is influenced by Bacillus-like Sf be it for pond surface, HH, or PSF derived water.

Docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid, has significant health benefits. Previous studies reported decreased levels of DHA and DHA-containing phosphatidylcholines in the brain of animals suffering from Alzheimer’s disease, the most common type of dementia; furthermore, DHA supplementation has been found to improve brain DHA levels and memory efficiency in dementia. Oil extracted from the seeds of Plukenetia volubilis (green nut oil; GNO) is also expected to have DHA like effects as it contains approximately 50% α-linolenic acid, a precursor of DHA. Despite this, changes in the spatial distribution of DHA in the brain of animals with dementia following GNO or DHA supplementation remain unexplored. In this study, desorption electrospray ionization imaging mass spectrometry (DESI-IMS) was applied to observe the effects of GNO or DHA supplementation upon the distribution of DHA in the brain of male senescence-accelerated mouse-prone 8 (SAMP8) mice, a mouse model of dementia. DESI-IMS revealed that brain DHA distribution increased 1.85-fold and 3.67-fold in GNO-fed and DHA-fed SAMP8 mice, respectively, compared to corn oil-fed SAMP8 mice. Memory efficiency in SAMP8 mice was also improved by GNO or DHA supplementation. In summary, this study suggests the possibility of GNO or DHA supplementation for the prevention of dementia.

From the conventional Bird’s eye, cancer initiation and metastasis are generally intended to be understood beneath the light of classical clonal genetic, epigenetic and cancer stem cell model. But inspite decades of investigation, molecular biology has shown hard success to give Eagle’s eye in unraveling the riddle of cancer. And it seems, tiring Tom runs in vague behind naughty Jerry.