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The hyperpolymorphic HLA genes play important roles in disease and transplantation and act as genetic markers of migration and evolution. A panel of 107 B‐lymphoblastoid cell lines (B‐LCLs) was established in 1987 at the 10th International Histocompatibility Workshop as a resource for the immunogenetics community. These B‐LCLs are well characterised and represent diverse ethnicities and HLA haplotypes. Here we have applied Pacific Biosciences’ Single Molecule Real‐Time (SMRT) DNA sequencing to HLA type 126 B‐LCL, including the 107 International HLA and Immunogenetics Workshop (IHIW) cells, to ultra‐high resolution. Amplicon sequencing of full‐length HLA class I genes (HLA‐A, ‐B and ‐C) and partial length HLA class II genes (HLA‐DRB1, ‐DQB1 and ‐DPB1) was performed. We typed a total of 931 HLA alleles, 895 (96%) of which were consistent with the typing in the IPD‐IMGT/HLA Database (Release 3.27.0, January 20, 2017), with 595 (64%) typed at a higher resolution. Discrepant types, including novel alleles (n = 10) and changes in zygosity (n = 13), as well as previously unreported types (n = 34) were observed. In addition, patterns of linkage disequilibrium were distinguished by four‐field resolution typing of HLA‐B and HLA‐C. By improving and standardising the HLA typing of these B‐LCLs, we have ensured their continued usefulness as a resource for the immunogenetics community in the age of next generation DNA sequencing. [ABSTRACT FROM AUTHOR]

The article offers information on the Nomenclature for factors of the Human leukocyte antigen system, update as of June 2017, including cell identification, accession number, and submitting author; and mentions that each sequence can be used to retrieve the files from libraries.

The article offers information on the Nomenclature for factors of the Human leukocyte antigen system, update as of June 2017, including cell identification, accession number, and submitting author; and mentions that each sequence can be used to retrieve the files from libraries.

Chimerism is defined as the presence of 2 or more than 1 genetically distinct cell populations in an organism. Dispermic chimeras are derived from the fertilization of 1 or 2 matured nuclei by 2 sperms. We here report detection of a healthy and phenotypically normal female with normal ABO red blood cell typing in whom dispermic chimerism was suspected after 3 alleles were identified at multiple human leukocyte antigen (HLA) loci using molecular HLA analysis. Molecular HLA typing showed the donor to have 3 HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 alleles in blood, saliva and nail samples. In addition, 3 of her 9 short tandem repeat loci also showed to have 3 distinct alleles in blood, nail and saliva specimens. In all investigations, the third alleles were attributed to a dual paternal contribution. This case represents a dispermic chimerism, with 2 paternal and 1 maternal haplotypes variably distributed throughout body tissues in a healthy and phenotypically normal female without abnormalities in erythrocyte ABO blood group. The origin of this chimerism is probably due to the fertilization of a single egg and its polar body, or a parthenogenetic egg, by 2 sperms. [ABSTRACT FROM AUTHOR]

The new HLA-B*39:01:01:04 allele differs from HLA-B*39:01:01 by a C → T substitution in intron 1. [ABSTRACT FROM AUTHOR]

The article presents several charts regarding human leukocyte antigen (HLA) sequences submitted to World Health Organization (WHO) Nomenclature Committee for Factors of the HLA System in August 2016 which includes new sequences, confirmatory sequences, and recently published sequences.

Two charts are presented listing newly assigned, and confirmatory human leukocyte antigen (HLA) sequences submitted to the World Health Organization Nomenclature Committee for nomenclature for Factors of the HLA System as of July 2016, with information on cell identification, and accession number.

The article presents several charts regarding human leukocyte antigen (HLA) sequences submitted to World Health Organization (WHO) Nomenclature Committee for Factors of the HLA System in September 2016 which includes new sequences, confirmatory sequences, and recently published sequences.

Several charts are presented related to the new HLA allele sequences submitted to the Nomenclature Committee since January 2016, confirmatory sequences from previously reported sequence, and recently published sequences.

Tables are presented that list the newly assigned sequences for factors of the human leukocyte antigen (HLA) system, as of October 2015.

Tables are presented that list the newly assigned sequences for factors of the human leukocyte antigen (HLA) system, as of November 2015.

Tables are presented that list the newly assigned sequences for factors of the human leukocyte antigen (HLA) system, as of December 2015.