251. Tissue engineering for cartilage repair: in vitro properties of a hyaluronan-derivative.
- Author
-
Grigolo B, Lisignoli G, Piacentini A, Fiorini M, Roseti L, De Franceschi L, Tognana E, Pavesio A, and Facchini A
- Subjects
- Adolescent, Adult, Cells, Cultured, Humans, Cartilage cytology, Hyaluronic Acid analogs & derivatives, Tissue Engineering methods
- Abstract
Association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage and bone repair. Such scaffolds should provide a performed three-dimensional shape, prevent cells from floating out of the defect, have sufficient mechanical strength, facilitate uniform spread of cells, and stimulate the phenotype of transplanted cells. Hyaff-11 is a recently developed hyaluronic-acid based biodegradable polymer, that has been shown to provide successful cell scaffolds for tissue-engineered repair. The aim of this study was to evaluate in vitro the potential of Hyaff-11 to support the growth of human chondrocytes and to maintain their original phenotype. Our data indicate that human chondrocytes seeded on Hyaff-11 express and produce collagen type II and aggrecan and downregulate the production of collagen type I. These results provide an in vitro demonstration of therapeutic potential of Hyaff-11 as a delivery vehicle in tissue-engineered repair of articular cartilage defects. more...
- Published
- 2003