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904 results on '"Lappin, Michael R."'

251. The 2006 American Association of Feline Practitioners Feline Vaccine Advisory Panel Report

Author

Richards, James R., primary, Elston, Thomas H., additional, Ford, Richard B., additional, Gaskell, Rosalind M., additional, Hartmann, Katrin, additional, Hurley, Kate F., additional, Lappin, Michael R., additional, Levy, Julie K., additional, Rodan, Ilona, additional, Scherk, Margie, additional, Schultz, Ronald D., additional, and Sparkes, Andrew H., additional

Published
2006
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269. Investigation of the induction of antibodies against Crandell-Rees feline kidney cell lysates and feline renal cell lysates after parenteral administration of vaccines against feline viral rhinotracheitis, calicivirus, and panleukopenia in cats

Author

Lappin, Michael R., primary, Jensen, Wayne A., additional, Jensen, Tracey D., additional, Basaraba, Randall J., additional, Brown, Cathy A., additional, Radecki, Steven V., additional, and Hawley, Jennifer R., additional

Published
2005
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282. Contributors

Author

Baneth, Gad, Barr, Stephen C., Bennett, Malcolm, Birkenheuer, Adam J., Breitschwerdt, Edward B., Chomel, Bruno B., Cohn, Leah A., Cramer, Sarah D., Davidson, Autumn P., Epstein, Steven, Foley, Janet E., Greene, Craig E., Grooters, Amy M., Gunn-Moore, Danièlle A., Hartmann, Katrin, Kapatkin, Amy S., Kidd, Linda, Lappin, Michael R., Lobetti, Remo, Luff, Jennifer A., Malik, Richard, Marks, Stanley L., Merkel, Lindsay K., Nagle, Terry M., Norris, Jacqueline M., Outerbridge, Catherine A., Papich, Mark G., Petersen, Christine A., Rankin, Shelley C., Saunders, Ashley B., Taboada, Joseph, Tasker, Séverine, Vincent-Johnson, Nancy, Weese, J. Scott, Westropp, Jodi L., and White, Stephen D.

Published
2014
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295. Development and Comparison of Sampling Techniques for a Broad Range, Semiquantitative Polymerase Chain Reaction Assay for Detection of Bacterial DNA in the Equine Uterus.

Author

Ferris, Ryan A., Veir, Julia K., Lappin, Michael R., and McCue, Patrick M.

Abstract

Abstract: Microbial culture from a double-guarded culture swab is commonly used to diagnose infectious endometritis. The objective of this study was to develop a quantitative polymerase chain reaction (qPCR) assay to detect a broad range of bacteria from equine uterine samples. Twenty-seven mares with a clinical history of endometritis had a double-guarded culture swab collected for analysis by qPCR and microbial cultures. An additional 12 mares had a uterine biopsy sample collected for qPCR analysis, microbial culture, and histopathology. Subsequently, a double-guarded culture swab for microbial culture and a cytology brush sample were also collected. The qPCR assay detected bacterial DNA in nine of 27 mares from a double-guarded swab and six of 12 mares from an endometrial biopsy. Positive microbial growth was detected in nine of 27 mares and four of 12 mares from a double-guarded culture swab. Bacterial DNA was detected in two of 27 mares and two of 12 mares without subsequent microbial growth. The simple presence of an organism's DNA allows for detection by nonculture-based systems, both live and dead organisms can be identified. In conclusion, the qPCR assay was determined to be a sensitive diagnostic technique for identifying pathogens associated with infectious endometritis. The primary application of the qPCR assay is detection of potential pathogenic bacteria in the uterus of a mare suspected of having infectious endometritis when a traditional microbial culture is negative. Further work is warranted to determine if mares positive for bacterial DNA and negative for microbial culture are affected clinically. [Copyright &y& Elsevier]

Published
2014
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296. Diagnostic utility of aqueocentesis and aqueous humor analysis in dogs and cats with anterior uveitis.

Author

Wiggans, K. Tomo, Vernau, William, Lappin, Michael R., Thomasy, Sara M., and Maggs, David J.

Subjects
AQUEOUS humor, IRIDOCYCLITIS, CYTOLOGY, DIAGNOSIS of dog diseases, CAT diseases, PERITONITIS, VETERINARY ophthalmology, DIAGNOSIS, VETERINARY diagnosis
Abstract

Objective To evaluate diagnostic utility of aqueous humor analysis in animals with anterior uveitis. Animals Client-owned dogs ( n = 12) and cats ( n = 10). Procedures Examination findings and diagnostic test results including aqueous humor cytology were compared. Results Disease duration prior to aqueocentesis was not significantly different between dogs with idiopathic anterior uveitis and those with an etiologic diagnosis, but was shorter in cats with feline infectious peritonitis ( FIP) than those with idiopathic uveitis. Microbial nucleic acids, antigens, or antibodies against them were seldom found in blood/serum; however, serum feline coronavirus titers ≥1:6400 were detected only in cats with FIP. Aqueous humor cytology was diagnostic in no cats and two dogs, both with neoplasia. Although aqueous humor contained predominantly neutrophils in cats with FIP and large reactive lymphocytes and plasma cells appeared more frequent in cats with idiopathic uveitis, neither clinical nor cytologic assessment of anterior chamber contents differed significantly between cats with idiopathic or FIP-associated uveitis. Cytologically assessed plasma cell number was correlated with keratic precipitates and disease duration. Clinically detectable hyphema and cytologic erythrocyte number were correlated. However, cytologic cell grades and clinical grade of flare or cell numbers within the anterior chamber were not correlated. Conclusions Aqueous humor cytology permitted diagnosis of neoplasia in dogs with anterior uveitis but was generally not helpful in cats. Poor correlation between clinical and cytologic assessment of cell numbers and type within the anterior chamber dictates that clinical grading should not be the sole criterion for electing to perform aqueocentesis. [ABSTRACT FROM AUTHOR]

Published
2014
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