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Background Maintaining stability and restoring the aesthetic appearance are the fundamental goals when managing zygomatic fractures. We aimed to evaluate the stability and anthropometric outcomes of zygomatic fracture patients who underwent two-point fixation involving the infraorbital rim and zygomaticomaxillary buttress via the transconjunctival and gingivobuccal approaches without any skin incisions. Methods We examined 15 zygomatic fracture patients who underwent two-point fixation during a 3-year period. Stability was evaluated using three-dimensional facial bone computed tomography. Superoinferior and anteroposterior displacement of the zygoma was quantified. The aesthetic appearance of the periorbital region was evaluated using indirect anthropometry with standardized clinical photographs. The ratios between the eye fissure height and width, and lower iris coverage ratio were used to evaluate aesthetical changes. The bony displacement and aesthetic ratios were analyzed using Wilcoxon or Friedman tests. The correlation between the preoperative zygoma position and anthropometric values was analyzed. Results The positions of the zygoma were similar to those on the contralateral side at the long-term follow-up. The preoperative anthropometric measurements on the fractured side differed from those on the contralateral side, although these values were close to the normal values at the long-term follow-up. Furthermore, we noted that the anteroposterior displacement strongly positively correlated with the lower iris coverage rate (Spearman's coefficient= 0.678, P=0.005). Conclusions Two-point fixation of zygomatic fractures achieved stable outcomes on long-term follow-up, and also appeared to be reliable in restoring the aesthetic appearance of the periorbital region.

Different researchers have recommended different lipoaspirate centrifugation speeds and times, probably due to the limits in fat cell viability assays. We assessed fat cell viability using a fluorescein diacetate and propidium iodide (FDA-PI) stain and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay after harvesting syringe liposuction and spun with different centrifugation speeds to determine the optimal conditions. Lipoaspirates, harvested from 13 donors, were transferred into a centrifuge tube and spun at 1000, 3000, and 4000 rpm for 3 minutes. Mature adipocytes and adipose stem cells were isolated and tested with a direct counting of FDA-PI-stained cells under fluorescence microscope and XTT assay. We incubated adipocytes and adipose stem cells for 1 day and 3 days, and we compared both of them with fresh samples to evaluate the influence of culturing condition on fat cell viability. Centrifugation speeds from 1000 rpm to 4000 rpm for 3 minutes showed no change in the percentage of adipocytes and adipose stem cell viability not only in the fresh samples but also in the cultured samples (1 day and 3 days). Centrifugation speeds under 4000 rpm do not change the percentage of fat cell viability. To differentiate viable cells from dying or dead mature adipocytes and oil accurately, combinations of viability tests are essential.

Isomorphic substitution, position replacement of one cation by another of similar size, leads to incorporation of a variety of cations into solid crystals without any significant changes to the primary crystal structures. To date, isomorphic substitution has been known to take place almost exclusively via co-crystallization of the cations during formation of the crystals. We report here the discovery of isomorphic substitution of trivalent metal cations for Ca2+ ions in portlandite crystals at room temperature as evidenced by the transient appearance of metastable phase, the formation of Ca-based layered double hydroxides at high pH, the distinct shift of suspension pH after phase transition, and the in situ topochemical reaction. This post-crystallization isomorphic substitution provides an innovative pathway for the synthesis of materials through chemical manipulation of crystals as well as a new insight into interpretation on their weathering and transformation processes.

We report the systematic experimental studies demonstrating that a graphene layer inserted at metal/n-Si(001) interface is efficient to explore interface Fermi-level pinning effect. It is confirmed that an inserted graphene layer prevents atomic interdiffusion to form an atomically abrupt Schottky contact. The Schottky barriers of metal/graphene/n-Si(001) junctions show a very weak dependence on metal work-function, implying that the metal Fermi-level is almost completely pinned at charge neutrality level close to the valence band edge of Si. The atomically impermeable and electronically transparent properties of graphene can be used generally to form an intact Schottky contact for all semiconductors.

Background For patients with neuropathy, vasculopathy, and impairment of wound healing, treatment of a diabetic foot ulcer poses many challenges. A large number of dermal analogues have been invented in an effort to overcome these challenges. Matriderm, a dermal analogue, is made from bovine collagen and elastin. This study was conducted in order to evaluate the effectiveness of Matriderm for treatment of diabetic foot ulcers, in comparison with skin grafting. Methods Sixty patients with diabetic foot ulcer were included in this prospective study. The average age of the patients, who had type II diabetes mellitus, was 58 years old. The patients were allocated to an experimental or control group with their consents. The patients were selected with their consent for inclusion in an experimental group and a control group. Patients in the experimental group received a Matriderm appliance and a split-thickness skin graft, while those in the control group received only a split-thickness skin graft. Results A shorter hospitalization period (7.52 weeks) was observed in the experimental group than in the control group (9.22 weeks), and a shorter period of time (8.61 weeks) was required for complete healing, compared with the control group (12.94 weeks), with statistical significance (P Conclusions Matriderm enables effective healing and improves elasticity in treatment of patients with diabetic foot ulcer.

Background Pedicled transverse rectus abdominis musculocutaneous flaps typically sacrifice the entire muscle. In our experience, the lateral strip of the rectus abdominis muscle can be spared in an attempt to maintain function and reduce morbidity. When the intercostal nerves are injured, muscle atrophy appears with time. The severed intercostal nerve was reinserted into the remnant lateral strip of the rectus abdominis muscle to reduce muscle atrophy. Methods The authors retrospectively reviewed 9 neurotized cases and 10 non-neurotized cases. Abdominal computed tomography was performed to determine the area of the rectus muscles. Electromyography (EMG) was performed to check contractile function of the remnant muscle. A single investigator measured the mean areas of randomly selected locations (second lumbar spine) using ImageJ software in a series of 10 cross-sectional slices. We compared the Hounsfield unit (HU) pre- and postoperatively to evaluate regeneration quality. Results In the neurotization group, 7 of 9 cases maintained the mass of remnant muscle. However, in the non-neurotization group, 8 of 10 lost their mass. The number of totally atrophied muscles in each of the two groups was significantly different (P=0.027). All of the remnant muscles showed contractile function on EMG. The 9 remaining remnant rectus abdominis muscles showed declined the HU value after surgery but also within a normal range of muscle. Conclusions Neurotization was found to be effective in maintaining the mass of remnant muscle. Neurotized remnant muscle had contractile function on EMG and no fatty degeneration by HU value.

Edge-adaptive interpolation is an effective technique to obtain a sharp high-resolution (HR) image from a low-resolution (LR) image. In this paper, we propose a new image interpolation method using edge-adaptive anisotropic Gaussian filters (AGFs). In the proposed method, the edge information of the LR image, which is the edge orientation and its energy, is first estimated using the modified Leung-Malik (LM) filter bank. Then, the edge information of the LR image is converted into that of the HR image by using a mapping function. Based on the HR edge information, edge-adaptive interpolation is performed using the AGF with variable shape and direction. Simulation results show that the proposed method objectively and subjectively outperforms conventional edge-based methods.

Background To minimize the inflammatory reaction and improve healing, a new modified dermal substitute composed of an atelocollagen, chondroitin-6-sulfate, and amniotic membrane (AM) was applied to full-thickness skin defects in a pig. Atelocollagen was extracted from bovine skin, and two modified dermal substitutes were generated according to the cross-linking type. Methods The AM-collagen dermal substitutes were characterized and compared with currently used dermal substitutes in a pig skin defect model. There were five experimental groups: dehydrothermal (DHT) cross-linking atelocollagen with the AM on the top (AM-DHT), DHT and chemical cross-linking atelocollagen with the AM on the top (AM-DHT/chemical), Terudermis, Integra, and AlloDerm. After 3×3 cm full-thickness skin defects on the back of a pig were created, each dermal substitutes dermal substitutes was randomly grafted on the defects. Two weeks after grafting, autologous partial-thickness skin was over-grafted on the neodermis. The take rate of the dermal substitutes, skin, and histological sections were all assessed at 1, 2, and 4 weeks postoperatively. Results More rapid healing and a higher take rate were evident in the AM-DHT and Terudermis groups. Histological examination revealed fewer inflammatory cells and more fibroblast hyperplasia in these two groups. Four weeks after surgery, the amount of newly formed collagen was significantly more appropriate in the AM-DHT group. Conclusions These observations provide supporting evidence that a newly developed amniotic-collagen dermal substitute may inhibit inflammatory reactions and promote wound healing.

Cattle are a natural reservoir of Shiga toxin-producing Escherichia coli (STEC) and have recently been recognized as a major source of Campylobacter jejuni contamination. While several factors are known to be associated with bacterial colonization, the underlying microbial factors have not been clarified. In this study, we characterized the fecal microbiota of dairy cattle (n = 24) using next-generation sequencing to elucidate the intestinal bacterial communities and the microbial diversity in relation to the presence of the foodborne pathogens STEC and C. jejuni (STEC-positive samples, n = 9; STEC-negative samples, n = 15; C. jejuni-positive samples, n = 9; and C. jejuni-negative samples, n = 15). While no significant differences were observed in alpha diversity between STEC-positive and STEC-negative samples, a high diversity index was observed in C. jejuni-positive samples compared to C. jejuni-negative samples. Nine phyla, 13 classes, 18 orders, 47 families, 148 genera, and 261 species were found to be the core microbiota in dairy cattle, covering 80.0-100.0% of the fecal microbial community. Diverse microbial communities were observed between cattle shedding foodborne pathogens and nonshedding cattle. C. jejuni-positive cattle had a higher relative abundance of Bacteroidetes (p = 0.035) and a lower relative abundance of Firmicutes (p = 0.035) compared to C. jejuni-negative cattle. In addition, while the relative abundance of 2 and 6 genera was significantly higher in cattle-shedding STEC and C. jejuni, respectively, the relative abundance of 3 genera was lower in both STEC- and C. jejuni-negative cattle. Our findings provide fundamental information on the bacterial ecology in cattle feces and might be useful in developing strategies to reduce STEC or C. jejuni shedding in dairy cattle, thereby reducing the incidence of STEC infection and campylobacteriosis in humans.

Background Facial hypoesthesia is one of the most troublesome complaints in the management of facial bone fractures. However, there is a lack of literature on facial sensory recovery after facial trauma. The purpose of this study was to evaluate the facial sensory recovery period for facial bone fractures using Neurometer. Methods Sixty-three patients who underwent open reduction of zygomatic and blowout fractures between December 2013 and July 2015 were included in the study. The facial sensory status of the patients was repeatedly examined preoperatively and postoperatively by Neurometer current perception threshold (CPT) until the results were normalized. Results Among the 63 subjects, 30 patients had normal Neurometer results preoperatively and postoperatively. According to fracture types, 17 patients with blowout fracture had a median recovery period of 0.25 months. Twelve patients with zygomatic fracture had a median recovery period of 1.00 month. Four patients with both fracture types had a median recovery period of 0.625 months. The median recovery period of all 33 patients was 0.25 months. There was no statistically significant difference in the sensory recovery period between types and subgroups of zygomatic and blowout fractures. In addition, there was no statistically significant difference in the sensory recovery period according to Neurometer results and the patients' own subjective reports. Conclusions Neurometer CPT is effective for evaluating and comparing preoperative and postoperative facial sensory status and evaluating the sensory recovery period in facial bone fracture patients.

Enterococcus spp. are normally present in the gastrointestinal tracts of animals and humans, but can cause opportunistic infections that can be transmitted to other animals or humans with integrated antibiotic resistance. To investigate if this is a potential risk in military working dogs (MWDs), we analyzed antibiotic resistance patterns and genetic relatedness of Enterococcus spp. isolated from fecal samples of MWDs of four different age groups. Isolation rates of Enterococcus spp., Enterococcus (E.) faecalis, and E. faecium, were 87.7% (57/65), 59.6% (34/57), and 56.1% (32/57), respectively, as determined by bacterial culture and multiplex PCR. The isolation rate of E. faecalis gradually decreased with age (puppy, 100%; adolescent, 91.7%; adult, 36.4%; and senior, 14.3%). Rates of resistance to the antibiotics ciprofloxacin, gentamicin, streptomycin, sulfamethoxazole/trimethoprim, imipenem, and kanamycin among Enterococcus spp. increased in adolescents and adults and decreased in senior dogs, with some isolates having three different antibiotic resistance patterns. There were indistinguishable pulsed-field gel electrophoresis patterns among the age groups. The results suggest that Enterococcus is horizontally transferred, regardless of age. As such, periodic surveillance studies should be undertaken to monitor changes in antibiotic resistance, which may necessitate modification of antibiotic regimens to manage antibiotic resistance transmission.

Background Among the various methods for correcting nasal deformity, the composite graft is suitable for the inner and outer reconstruction of the nose in a single stage. In this article, we present our technique for reconstructing the ala and columella using the auricular chondrocutaneous composite graft. Methods From 2004 to 2011, 15 cases of alar and 2 cases of columellar reconstruction employing the chondrocutaneous composite graft were studied, all followed up for 3 to 24 months (average, 13.5 months). All of the patients were reviewed retrospectively for the demographics, graft size, selection of the donor site and outcomes including morbidity and complications. Results The reasons for the deformity were burn scar (n=7), traumatic scar (n=4), smallpox scar (n=4), basal cell carcinoma defect (n=1), and scar contracture (n=1) from implant induced infection. In 5 cases of nostril stricture and 6 cases of alar defect and notching, composite grafts from the helix were used (8.9×12.5 mm). In 4 cases of retracted ala, grafts from the posterior surface of the concha were matched (5×15 mm). For the reconstruction of the columella, we harvested the graft from the posterior scapha (9×13.5 mm). Except one case with partial necrosis and delayed healing due to smoking, the grafts were successful in all of the cases and there was no deformity of the donor site. Conclusions An alar and columellar defect can be reconstructed successfully with a relatively large composite graft without donor site morbidity. The selection of the donor site should be individualized according to the 3-dimensional configuration of the defect.

The intrinsic factors associated with delayed wounds are ischaemia, infection, the presence of necrotic tissue and the presence of foreign bodies. This study was designed to create adaptable intrinsic factors induced delayed wound healing model in the pig, similar to clinical conditions. Four pigs were used in this study. Ten 4 × 4 cm fresh full-thickness skin defect wounds were made on the back of each pig. Double-flanged blocks made of silicon, a material that induces foreign body reactions, tissue ischaemia and causes local wound infection, were inserted into half of the wounds (n = 5) and removed at weeks 1, 2 or 3. The other wounds (n = 5) were left open for control purposes. Biopsies were obtained at weeks 1-4 for histological examinations and to evaluate inflammatory cytokines, which included tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6. A Periscan PIM 3 Laser was used to measure degrees of ischaemia. A Visitrak Digital(®) device was used to measure the sizes of unhealed wounds. After 3 weeks, unhealed mean wound sizes were 30·72 ± 3·36 and 2·91 ± 1·51 cm(2) , in the experimental and control groups, respectively. Laser Doppler showed limited perfusion at wound margins in wounds containing silicone blocks. Histological findings corresponding to the severity of chronic inflammation were markedly higher and persisted until week 3 in the experimental group. The levels of IL-1 and TNF-α in the third week were statistically significantly higher in the model group. Also, in the fourth week, the level of TNF-α in the model group was significantly higher. Epithelialisation was not complete at the end of the fourth week despite removing silicone blocks in the experimental group. On the basis of macroscopic and histological evaluations and molecular assessments, the described wound model has the characteristics of wound ischaemia and necrosis and persistent inflammation, which are very similar to delayed wounds of patients.

Background Axillary osmidrosis is characterized by an unpleasant odor, profuse sweating, and in some instances, staining of clothes that may socially and psychologically impair affected individuals. Various types of surgical procedures have been developed for the treatment of axillary osmidrosis. This study was undertaken to evaluate the effectiveness of subcutaneous pulsed neodymium: yttrium-aluminum-garnet (Nd-YAG) laser treatment for the treatment of axillary osmidrosis. Methods Twenty-nine patients with axillary osmidrosis were included in this study. Patients were categorized according to the results of an axillary malodor grading system, and a subcutaneous pulsed Nd-YAG laser was applied to all patients. The treatment area for the appropriate distribution of laser energy was determined using the iodine starch test (Minor's test) against a grid pattern composed of 2×2 cm squares. The endpoint of exposure was 300 to 500 J for each grid, depending on the preoperative evaluation results. The results were evaluated by measurement of axillary malodor both pre- and postoperatively using the grading system and iodine starch test. Results The average follow-up period was 12.8 months. Nineteen patients had a fair-to-good result and ten patients had poor results. The postoperative Minor's test demonstrated that there were remarkable improvements for patients with mild to moderate symptoms. Complications including superficial second degree burns (n=3) were treated in a conservative manner. A deep second degree burn (n=1) was treated by a surgical procedure. Conclusions Subcutaneous pulsed Nd-YAG laser has many advantages and is an effective noninvasive treatment for mild to moderate axillary osmidrosis.

Well-defined Au/ZnO nanoparticle composites were prepared by modifying ZnO with preformed Au nanoparticles protected with bifunctional glutathione ligand. In this approach, the Au nanoparticles were highly monodisperse and their loading on ZnO surface could be precisely controlled by the anchoring conditions. Steady-state and time-resolved photoluminescence of the composites revealed the ability of the Au nanoparticles to efficiently extract conduction band electrons from the photoexcited ZnO. The composites exhibited strongly enhanced photocatalytic activity without requiring thermal activation process in degrading organic substrates in both oxidative and reductive pathways. A clear correlation between the photocatalytic activity and the Au loading was found for both oxidative and reductive photocatalytic reactions. These results demonstrate that thiolate-protected AuNPs can significantly enhance the charge separation by extracting electrons from the photoexcited ZnO and consequently improve the photocatalytic activity of the composites.

In this paper, we propose a gradient saliency based spatio-temporal video noise reduction method for digital TV. In the proposed method, the gradient saliency, which represents the perceptually distinct image structure, is utilized to accurately identify the edge in a noisy image. The proposed spatial filtering uses the directional Gaussian filter at edge pixels and the isotropic filter at non-edge pixels. In order to suppress the noise in video sequences, we adopt a spatio-temporal structure in which temporal filtering is performed using temporal correlation between two successive frames, and then the temporal filtered result is processed by the proposed gradient saliency noise reduction filter for further noise reduction. The experiment results demonstrate that the proposed method achieves substantial improvements in terms of both visual quality and PSNR as compared with the conventional methods.

In this paper, we propose a fast and accurate training based auto-focus (AF) method for mobile-phone cameras. Given a set of training data, the proposed method collects feature vectors consisting of focus value increment ratio. The representative feature vectors corresponding to every possible best in-focus lens position (BILP) are stored in the database. In the proposed training based AF method, the BILP is obtained by comparing the input feature vector with the representative feature vectors in the database. To further enhance the accuracy of the proposed AF method, we also introduce a new focusing window that is effective in detecting the target object. The experimental results show that the proposed method can accurately estimate the BILP faster than the existing methods.

The luminescence of quantum-sized metal clusters has enthralled the scientific community in recent years. In this study, ultrafast luminescence dynamics of hexanethiol (C6S)- and glutathione (GS)-protected Au25 clusters are investigated with time-resolved luminescence spectroscopy. The focus of the present investigation is to understand the dynamics of higher excited states and also the relaxation of core Au states to Au semi-ring states in Au25L18 (“L” is the protecting ligand) clusters. Comparative luminescence measurements on larger monolayer-protected gold clusters (2.2 nm Au(C6S) and 2.2 nm Au(GS)) and gold nanoparticles (3 nm Au(C6S) and 13 nm Au(citrate-stabilized)) are also carried out. The investigated Au25L18 clusters have shown a low quantum-yield visible photoluminescence in addition to near-infrared luminescence, which is used as a probe to follow the dynamics of core Au states. The luminescence decay traces of Au25L18 clusters have shown unique ultrafast growth and decay kinetics that are ab...

It is believed that the subcutaneous fat of the skin compositae grafts acts as a mechanical barrier limiting vascularisation. This study aims to determine a new mechanism associated with compositae graft take. Ten 3×3 cm rectangular full-thickness skin compositae grafts on the back of a pig were taken and then randomly changed to another place. Silicone sheets were then inserted between the graft and the recipient to block the direct contact of the dermis at the lateral surface of the graft and control the number of surface contacts. The take rate was measured using the digital VISITRAK(®). The microcirculation of the graft was evaluated by microangiograms using a latex-lead oxide solution. There was a 20.5% graft take in all four surface-blocked groups. The microangiograms showed vessel connections not only between the vessels of the dermis, but also between the subcutaneous fat of the graft and perforators from the basal surface of the wound. The subcutaneous layer does not produce a barrier but works as a significant source of vessel communication. Direct vessel-to-vessel anastomosis between the vessels of the subcutaneous fat at the graft and the basal surface of the recipient wound are another important mechanism for the success of compositae grafts.

TiO2 nanoparticle photocatalysts modified with uniform gold nanoparticles are prepared by anchoring bifunctional glutathione-coated monolayer-protected gold clusters (MPCs) onto TiO2. In this method, the MPC loading on TiO2 can be precisely controlled in the range of 1−5 wt % without incurring size change upon anchoring. The photocatalytic degradation of Uniblue A (UBA) is carried out with thus prepared MPC−TiO2 composites, which shows, however, no enhancement upon MPC anchoring. The MPC−TiO2 composites are thermally treated to activate the catalytic activity. When the MPC−TiO2 composites are calcined at 250 °C, the glutathione ligand on gold surface is partially removed and the photocatalytic activity of the composites significantly increases, highlighting the role of gold in the photocatalytic reactions. However, when the calcination temperature is raised to 400 °C, the photocatalytic activity of the composites drastically decreased. X-ray photoelectron spectroscopy analysis of the calcined composites r...

The purpose of this study was to determine the late decline in viability of fat cells over time for fat tissue stored at -15 degrees C and -70 degrees C after harvest from abdominal liposuction. A total of 16 females were recruited for this study. The viability of fat cell specimens was measured after freezing for 1, 3, 7, 14, 28, and 56 days. A number of viable mature adipocytes were evaluated by fluorescence microscopy after staining with fluorescein diacetate and propidium iodide. The glycerol-3-phosphate dehydrogenase activity was measured in lipoaspirates before digestion and the XTT reduction assay was performed. In addition, the XTT reduction assay was also performed on isolated lipocytes and preadipocytes.The viability of mature adipocytes was very low for both the -15 degrees C and -70 degrees C samples after 1 day of freezing (13.3% +/- 7.4% and 12.6% +/- 6.3%, respectively). There was no statistically significant difference between the samples stored at the 2 temperatures. The GPDH activity of the lipoaspirates frozen, for 1 day, at -15 degrees C and -70 degrees C was 25.1% +/- 10% and 28.7% +/- 11%, respectively. For the XTT test, the fractional enzyme activity of the lipoaspirates frozen, for 1 day, at -15 degrees C and -70 degrees C was 30.0% +/- 10.9% and 36.1% +/- 12.3%, respectively. In addition, the adipocytes had low activity from day one: 15.4% +/- 7.2% at -15 degrees C and 11.5% +/- 5.6% at -70 degrees C. Furthermore, the preadipocytes had a low activity of 8.0% +/- 6.0% at -15 degrees C and 8.6% +/- 3.8% at -70 degrees C. At 8 weeks, there were few viable mature adipocytes and the activity of the cells was very low by XTT and GPDH testing.The results of this study showed that the viability of adipocytes declined rapidly after frozen storage for 1 day at both -15 degrees C and -70 degrees C, and decreased gradually in storage after 8 weeks; at which time only approximately 5% of the fat cells were alive. These findings suggest that the present fat preservation storage techniques using a -15 degrees C freezer or a -70 degrees C deep freezer are both inadequate to maintain the viability of fat cells.

Multiwall carbon nanotube (MWNT)/manganese oxide (MnO2) nanocomposite ultrathin film electrodes have been created via redox deposition of MnO2 on layer-by-layer (LbL)-assembled MWNT films. We demonstrate that these LbL-assembled MWNT (LbL-MWNT)/MnO2 thin films consist of a uniform coating of nanosized MnO2 on the MWNT network structure using SEM and TEM, which is a promising structure for electrochemical capacitor applications. LbL-MWNT/MnO2 electrodes yield a significantly higher volumetric capacitance of 246 F/cm3 with good capacity retention up to 1000 mV/s due to rapid transport of electrons and ions within the electrodes. The electrodes are generated with two simple aqueous deposition processes: the layer-by-layer assembly of MWNTs followed by redox deposition of MnO2 at ambient conditions, thus providing a straightforward approach to the fabrication of high-power and -energy electrochemical capacitors with precise control of electrode thickness at nanometer scales.

The purpose of this study was to investigate the efficacy of cilostazol for prevention of thrombosis in microsurgical anastomosis. We used not only a patency test, but also ultrasonography to evaluate the blood flow volume (FV) more accurately and objectivity. In addition, we evaluated the accuracy and sensitivity of the patency test. Thrombogenic anastomoses were performed on rat external iliac arteries. Forty-six rats were randomly allocated into the following 5 experimental groups: negative control, without any surgery and medication; control, received distilled water orally; group A, received aspirin orally; group B, received cilostazol orally; and group C, received aspirin and cilostazol orally 3 hours before a thrombogenic artery anastomosis. The artery was evaluated by FV using ultrasonography and a patency test 10, 30, and 120 minutes after clamp removal. The FV in group C was significantly larger than the control group 30 minutes after clamp removal. The FVs in groups A, B, and C were significantly larger than the control group 120 minutes after clamp removal. The FV decreased in all groups with time. The patency rate between the control group and group C 120 minutes after clamp removal was significantly different. There were 8 false negatives in a total of 114 patency tests. The total accuracy and sensitivity of the patency test were 93.0% and 92.7%, respectively. Cilostazol plus aspirin therapy is recommended for reducing thrombosis and increasing FV without increasing the risk of bleeding during microsurgery. The patency test cannot evaluate the state of the anastomotic vessels accurately and objectively.

Summary Objectives The aim of this study was to standardize clinical photogrammetric techniques, and to compare anthropometry with photogrammetry. Material and methods To standardize clinical photography, we have developed a photographic cephalostat and chair. We investigated the repeatability of the standardized clinical photogrammetric technique. Then, with 40 landmarks, a total of 96 anthropometric measurement items was obtained from 100 Koreans. Ninety six photogrammetric measurements from the same subjects were also obtained from standardized clinical photographs using Adobe Photoshop version 7.0 (Adobe Systems Corporation, San Jose, CA, USA). The photogrammetric and anthropometric measurement data (mm, degree) were then compared. A coefficient was obtained by dividing the anthropometric measurements by the photogrammetric measurements. Results The repeatability of the standardized photography was statistically significantly high (p = 0.463). Among the 96 measurement items, 44 items were reliable; for these items the photogrammetric measurements were not different to the anthropometric measurements. The remaining 52 items must be classified as unreliable. Conclusions By developing a photographic cephalostat and chair, we have standardized clinical photogrammetric techniques. The reliable set of measurement items can be used as anthropometric measurements. For unreliable measurement items, applying a suitable coefficient to the photogrammetric measurement allows the anthropometric measurement to be obtained indirectly.

In general, since the noise deteriorates the interpolation performance in digital images, it is effective to employ denoising prior to the interpolation. In this paper, we propose a novel interpolation framework in which denoising and image sharpening methods are embedded. In the proposed framework, the image is first decomposed using the bilateral filter into the detail and base layers which represent the small and large scale features, respectively. The detail layer is adaptively smoothed to suppress the noise before interpolation and an edge-preserving interpolation method is applied to both layers. Finally, the high resolution image is obtained by combining the base and detail layers. Experimental results show that the proposed algorithm outperforms the conventional methods while suppressing blurring and jagging.

Septal extension grafts are performed in order to correct the conformation of the nasal tip and columella. The more strongly the graft is fixed to the columellar L-strut of the septal cartilage, the better is the outcome of grafting. This article reports the development of a vertical figure-of-eight locking suture technique and its biomechanical comparison with other suture techniques. Pieces of porcine rib cartilage 20 x 15 x 1 mm (or 2 mm) in size were used for the study. Two pieces of cartilage were fixed using two simple interrupted sutures in the first group, two horizontal figure-of-eight locking sutures in the second group and the vertical figure-of-eight locking suture in the third group. Shearing, buckling and tensile forces were measured with respect to the direction of forces that occur in vivo. The mean shearing force for cartilages that were 1 mm in thickness was 0.70, 0.74 and 1.04 N (P=0.056) in the first, second and third group, respectively. The mean buckling force was 1.10, 1.09 and 1.39 N (P=0.030), and the mean tensile force was 1.86, 1.78 and 2.24 (P=0.042), respectively. For cartilages that were 2 mm in thickness, the mean shearing force was 1.19, 1.29 and 1.54 N (P=0.216), the mean buckling force was 1.74, 1.50 and 2.25 (P=0.002) and the mean tensile force was 2.00, 1.81 and 2.29 (P=0.023) in the first, second and third group, respectively. The vertical figure-of-eight locking suture technique was developed as an alternative technique for suturing a septal extension graft to the columellar L-strut, and it enables firm fixation with the use of the fewest number of stitches.

Correction for ‘An elastomer for epidermal electronics with adjustable adhesion force and stretchability obtained via a reverse-micelle-induced process’ by Junhyung Kim et al., J. Mater. Chem. C, 2018, 6, 2210–2215.

Gold nanoparticle/multiwall carbon nanotube (AuNP/MWNT) thin films were fabricated by using the layer-by-layer (LBL) assembly technique. The microstructure of the film was characterized by TEM and AFM, which showed that the MWNTs in the AuNP/MWNT LBL films are randomly entangled to form a dense network, in which the AuNPs are intercalated. The plasmonic properties and electrocatalytic activity of these films were studied as a function of number of bilayers. Surface plasmon resonance of the AuNP/MWNT films increased linearly and exhibited a red shift with increasing number of bilayers as a result of increasing sizes of the AuNP agglomerates in the films. The AuNP/MWNT films were electrochemically active toward methanol oxidation reaction in alkaline solution. Linear sweep voltammograms revealed a systematic increase in the methanol oxidation current with increasing number of bilayers. This work offers promise to the development of efficient electrodes based on metal nanoparticle/MWNT nanoassembly for elect...

In low bit-rate video transmission, the payload of a single packet can often contain a whole coded frame due to the high compression ratio in both spatial and temporal domains. Thus, the loss of a single packet can lead to the loss of a whole video frame. In this paper, we propose a novel error concealment algorithm that can effectively reconstruct the lost frame and protect the quality of video streams from the degradation caused by propagation errors. The proposed algorithm employs a bilateral motion estimation scheme where the weighted sum of the received motion vectors (MVs) in the neighboring frames is utilized to construct the MV field for the concealed frame. Unlike the conventional algorithms, the proposed scheme does not produce any overlapped pixel and hole region in the reconstructed frame. The proposed algorithm can be applied not only to the case of single frame loss but also adaptively extended to the case of multiframe loss. Experimental results show that the proposed algorithm outperforms other conventional techniques in terms of both peak signal-to-noise ratio (PSNR) performance and subjective visual quality.

Summary Using the conchal cartilage as a graft has been widely accepted, but the deformities that can result have been underestimated. The deformities range from a visible scar to the total collapse of the ear framework. A simplified technique of harvesting the maximal amount of the conchal cartilage graft is suggested, based on the results of 16 consecutive cases. The technique involved (1) using a postauricular incision to minimise visible scars, (2) harvesting the entire cymba concha and cavum concha separately, with at least 5mm of the helical crus, leaving a lateral extension as a strut between them, as well as a 2mm outer rim along the conchal wall, and (3) by using a tie-over bolster dressing that can serve as a mould for the conchal bowl. This technique has been used on 16 patients with neither contour irregularity nor any deformity, from a case of a step deformity to the total collapse of the ear.

Etanercept is a fully humanized soluble tumor necrosis factor (TNF)-alpha receptor that competitively inhibits the interaction of TNF-alpha with cell-surface receptors. It was approved as monotherapy for psoriasis in the USA in 2004, but in Korea, no clinical reports on its use for psoriasis are available. We performed a retrospective analysis of 26 moderate-to-severe psoriasis patients who had been treated with etanercept. Patients received twice-weekly injections of 25 mg etanercept s.c. for at least 4 weeks. When the patients achieved a 50% reduction of the psoriasis area severity index (PASI 50) they received once-weekly injections, then biweekly injections were provided for maintenance. Patients were evaluated biweekly by clinical photographs and PASI scoring. Treatment efficacy was as follows. A PASI 75 was achieved in 14 patients (54%) and the mean number of injections before achieving a PASI 75 was 10 +/- 7.5. Patients whose initial PASI was less than 10 (iPASI10) showed an earlier response (2.6 +/- 1.3 weeks) and a higher PASI 75 rate (63%), than with iPASIor = 10 (6.9 +/- 4.5 weeks, 50%). Eight patients (31%) received additional phototherapy or systemic therapy because of insufficient responses or for faster improvements and they were excluded in the efficacy evaluation. Adverse events were observed in eight patients (31%), but were not serious. This is the first report on the effectiveness of low-dose etanercept regimen on Asian psoriasis patients. Results in this study showed that low-dose etanercept therapy is effective for moderate-to-severe Asian psoriasis patients, and it may be a valuable treatment option even for relatively moderate psoriasis patients not responsive to conventional treatment. In addition, the medical cost was relatively low compared to that of the standard regimen for white patients.

Boundary matching algorithm (BMA) and decoder motion vector estimation (DMVE) are two well-known temporal error concealment methods using the matching-based approach. In these two methods, the motion vector of each missing block is estimated by choosing one among candidate motion vectors which minimizes a sum of absolute differences (SAD) between boundary pixels of the corrupted macroblock. In general, the performance of DMVE is better than that of BMA. However, depending on the location or pattern of the corrupted block, BMA produces higher visual quality than DMVE. In this paper, we propose two types of hybrid error concealment methods; switching method and blending method. The switching method chooses one of two results obtained by BMA and DMVE based on the normalized SAD values. In the blending method, the weighted sum of the results concealed by the aforementioned two methods is utilized to improve the performance of error concealment. In order to reduce blocking artifacts further, the modified overlapped-block motion compensation is adaptively applied to the concealed blocks. Simulation results show that the proposed methods outperform other techniques in terms of subjective visual quality as well as PSNR performance.

The photoluminescence quenching of a CdSe quantum dot by hexanethiolate-monolayer-protected gold clusters (MPCs) with core diameters of 1.1−4.9 nm is described. Experimental evidence suggests that the quenching occurs via an energy transfer mechanism. The energy transfer quenching efficiencies of MPCs were compared using Stern−Volmer plots. The quenching constant (KQ) obtained from the slope of the plot shows an enormous increase from 2.5 × 105 to 2.3 × 108 M-1 by nearly 1000-fold as the core size increases from 1.1 to 4.9 nm. The origin of the size dependence is considered. There is a remarkable linear correlation found between the quenching constant and the MPC core volume. This correlation suggests that the energy transfer quenching efficiency is governed by the absorption cross-section of the MPC quencher that scales as its core volume.

Error concealment at a decoder is an efficient method to reduce the degradation of visual quality caused by channel errors. In this paper, we propose a novel spatio-temporal error concealment algorithm based on the spatial-temporal fading (STF) scheme which has been recently introduced. Although STF achieves good performance for the error concealment, several drawbacks including blurring still remain in the concealed blocks. To alleviate these drawbacks, in the proposed method, hybrid approaches with adaptive weights are proposed. First, the boundary matching algorithm and the decoder motion vector estimation which are well-known temporal error concealment methods are adaptively combined to compensate for the defect of each other. Then, an edge preserved method is utilized to reduce the blurring effects caused by the bilinear interpolation for spatial error concealment. Finally, two concealed results obtained by the hybrid spatial and temporal error concealment are pixel-wisely blended with adaptive weights. Experimental results exhibit that the proposed method outperforms conventional methods including STF in terms of the PSNR performance as well as subjective visual quality, and the computational complexity of the proposed method is similar to that of STF.

We used two flow-through arterialised venous free flap transfers with the long saphenous vein to reconstruct major arteries and injured skin and soft tissues in the upper extremity. Operating time was reduced, only one donor site was used, and reconstruction of a long arterial defect (24-25 cm) was possible.

BACKGROUND Objective studies evaluating the relevant factors that affect skin graft changes in color over time have yet to be published. OBJECTIVE Therefore, the patterns of the grafted skin's color changes and the presence of relevant factors were analyzed statistically. MATERIALS AND METHODS The study included 107 skin grafts in 107 subjects. Using a chromameter, the colors of skin graft, the area adjacent to the recipient site, and the donor site were measured. The grafted skin's color changes and the color difference were analyzed with respect to several factors. RESULTS Over time, the grafted skin became lighter, redness decreased, yellowness increased, and the color difference decreased. As the donor site was lighter, the grafted skin was lighter and less red. The grafted skin was lighter in females than in males. The skin graft type was not related to the lightness and the redness. The grafted skin was lightest in the upper arm and darkest in the lower leg. The grafted skin was lighter in Fitzpatrick Skin Type III, followed by Type IV and then Type V. CONCLUSION The factors that affected the skin graft's lightness were time, the lightness of the donor, sex, the hand, the foot, and Fitzpatrick skin type.

Summary The tongue has important roles in deglutition, speech, airway protection, taste, and sexual function, and its amputation is very serious. Using supermicrosurgical techniques, we replanted successfully a patient's tongue, the distal part of which had been amputated by the bite of another person, through the anastomosis of a blood vessel that was only 0.7–0.8mm. The result was that the state of the replanted tongue was relatively good both functionally and aesthetically, and the patient was very satisfied.

In many applications including Internet video broadcast and interactive TV, video streams are transmitted over various networks. In order to fulfil the requirement of the users who want to watch a part of the video stream, start playback at a random location, and jump to another location, the service provider has to implement the random access functionality. In addition, when the video stream is transmitted over error-prone networks like wireless LAN, the random access point can be used as a recovery point which enables the user device to refresh the decoding process corrupted by the transmission error. In this paper, we propose a simple transcoding algorithm inserting the random access point in the pre-encoded video stream. The multimedia server adopting the proposed transcoding algorithm can support the error-resilient transmission as well as the playback at random location. Experimental results show that the proposed method can gain significant reduction in the complexity without introducing degradation on visual quality.

The nose of most Koreans is characterised by a low nasal dorsum, retracted columella, and an acute columella-labial angle. For the surgical correction of the tip and columella, a complete septal extension graft, along with augmentation rhinoplasty has been developed. However, the use of this type of graft is frequently problematic because the septal cartilage is not large enough. In a study involving 10 cadavers, a complete septal extension graft was achieved in two of them by using septal cartilage harvested according to standard techniques. Therefore, the septal cartilage was harvested, leaving a 5-mm L-shaped strut, and a complete septal extension graft was implanted. The present report describes the results obtained in 34 patients and offers an analysis of the results as judged by the columella-labial angle and three proportional indices (nose height index, nasal bridge length index, and nasal tip projection index), measured by photogrammetry. The postoperative values obtained in these four categories increased significantly compared to the preoperative ones, thus confirming that the projection of tip was augmented, the nose was lengthened, and the columella was advanced caudally. Moreover, these positive outcomes were maintained during long-term follow-up, and no side effects, such as saddle nose deformity, were reported.

A stencil lithography technique has been developed to fabricate organic-material-based electronic devices with sub-micron resolution. Suspended polymethylmethacrylate (PMMA) membranes were used as shadow masks for defining organic channels and top electrodes. Arrays of pentacene field effect transistors (FETs) with various channel lengths from 50 μm down to 500 nm were successfully produced from the same batch using this technique. Electrical transport measurements showed that the electrical contacts of all devices were stable and the normalized contact resistances were much lower than previously studied organic FETs. Scaling effects, originating from the bulk space charge current, were investigated by analyzing the channel-length-dependent mobility and hysteresis behaviors. This novel lithography method provides a reliable means for studying the fundamental transport properties of organic materials at the nanoscale as well as enabling potential applications requiring the fabrication of integrated organic nanoelectronic devices.

In minimal incision methods for removing an epidermal cyst, the cyst contents and lining are generally manually squeezed out through a small opening using lateral pressure. However, lateral pressure can cause unintended rupture, such as intracavitary rupture, and fragmentation of the lining into small pieces that may cause infection and recurrence. Additionally, it is difficult to completely remove cysts that are larger than 2 cm. In this paper, we present a small-incision method using negative-pressure suction instead of lateral pressure for excision of large epidermal cysts (>2 cm in diameter). Between 2006 and 2015, 19 patients with large epidermal cysts were included in this study. The most common locations of the cysts were the face and neck (8 cases), back (5 cases), buttocks (4 cases), and axilla (2 cases). The mean diameter of the cysts was 4.4 cm, and the largest cyst was 14 cm in diameter. The incision length ranged from 0.7–1.8 cm (mean, 1.0 cm). The mean operative time was 28 min. The mean follow-up period was 13.2 months. During this period there were no recurrences. One patient developed a hematoma, but there were no other complications. We used negative-pressure suction instead of lateral pressure with a minimal surgical technique. We were able to completely remove the lining of a large epidermal cyst through a small incision. This journal requires that authors to assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

Purpose The visible Z-shaped upper lip scar that occurs after the Tennison and Randall triangular flap technique remains a cleft stigma. Herein, we present our curvilinear transformation technique for the Z-shaped upper lip scar by diamond-shaped excision and evaluate the results using photogrammetric analyses. Patients and Methods From 1997 to 2006, 23 patients with secondary cleft lip deformity with the visible Z-shaped upper lip scar underwent correction with the technique. The scar was excised in the diamond shape above the muscle. After curvilinear closure, the elongated length of the upper lip was excised just below the nostril sill, as the measured Cupid's bow height discrepancy. The result was assessed by the authors' standardized photogrammetry technique. Results There was a statistically significant decrease between the preoperative central limb of the Z-shaped scar and the width of the postoperative curvilinear upper lip scar. The pre- and postoperative Cupid's bow height differences were not statistically significant. Conclusions The curvilinear transformation of the Z-shaped scar is an efficient procedure that provides (1) a significant decrease in the width of an upper lip scar to make it less conspicuous, (2) incorporation of the scar into the philtral column, (3) a biconcave natural philtral column shape in frontal view, and (4) formation of the natural concave philtral contour in profile view.