336 results on '"Hidetaka Katabuchi"'
Search Results
252. Expression of variant luteinizing hormone/chorionic gonadotropin receptors and degradation of chorionic gonadotropin in human chorionic villous macrophages
- Author
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Hidetaka Katabuchi, Takashi Minegishi, N. Sonoda, R. Nishimura, Hitoshi Okamura, Takashi Ohba, and Hironori Tashiro
- Subjects
endocrine system ,medicine.medical_specialty ,medicine.drug_class ,Cell ,Molecular Sequence Data ,Biology ,Chorionic Gonadotropin ,Cell Line ,Pregnancy ,Internal medicine ,medicine ,Humans ,RNA, Messenger ,Receptor ,Base Sequence ,Macrophages ,Obstetrics and Gynecology ,Genetic Variation ,Transfection ,Luteinizing Hormone ,Receptors, LH ,Immunohistochemistry ,In vitro ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,Gene Expression Regulation ,Cell culture ,Chorionic villi ,Female ,Gonadotropin ,Chorionic Villi ,Luteinizing hormone ,Developmental Biology - Abstract
Human gonads and non-gonadal organs/tissues express luteinizing hormone/chorionic gonadotropin (LH/CG) receptors. This study aimed to identify the LH/CG receptors and to clarify their function in human placental chorionic villous macrophages. Macrophages as well as syncytiotrophoblasts of human chorionic villous tissues were immunohistochemically positive for LH/CG receptor throughout gestation. By reverse transcription-nested polymerase chain reaction methods, villous macrophages were shown to express a variant type of LH/CG receptor, the sequencing of which revealed a deletion of exon 9. For experiments in vitro, a monocyte-macrophage cell line, THP-1, was transfected with vector alone, wild-type LH/CG receptor, and exon 9-deleted LH/CG receptor after phorbol 12-myristate 13-acetate (PMA) treatment. Non-PMA-treated THP-1 cells transfected with vector alone were also examined. THP-1 cells expressed exon 9-deleted LH/CG receptor after treatment with PMA. After the cells of the four groups were cultured in medium containing intact human CG (hCG), the concentrations of hCG and its beta-core fragment (beta-CF) were measured in the supernatant of the culture medium and in the cell cytosol. Time-dependent hCG uptake was observed in both non-PMA-treated and PMA-treated THP-1 cells, suggesting that the variant receptor is not directly involved in the ingestion of hCG. The degradation of hCG and excretion of beta-CF were progressed in PMA-treated cells but not in the un-treated cells. In the cell cytosol, the ratio of beta-CF and hCG concentrations (beta-CF/hCG) was significantly higher in the PMA-treated cells than in non-PMA-treated cells; however, it did not differ between the PMA-treated cells transfected with exon 9-deleted receptor and those transfected with vector alone. Macrophages may express the variant receptor in order to recognize the intracytoplasmic hCG and transport it to the lysosome. Among the two PMA-treated cells, the ratio was lower in those transfected with wild-type receptor. The expression of the variant receptor may modulate the degradation of hCG but be reduced by expression of the wild-type receptor in its lacking macrophages. Our data suggest a potentially important role for exon 9-deleted LH/CG receptors expressed in human placental villous macrophages in the local metabolism of hCG.
- Published
- 2004
253. Pathophysiological Dynamics of Human Ovarian Surface Epithelial Cells in Epithelial Ovarian Carcinogenesis
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Hitoshi Okamura and Hidetaka Katabuchi
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endocrine system diseases ,Cancer ,Ovary ,Disease ,Biology ,medicine.disease ,medicine.disease_cause ,Pathogenesis ,medicine.anatomical_structure ,Immunology ,medicine ,Cancer research ,Receptor ,Carcinogenesis ,Immortalised cell line ,Mesothelial Cell - Abstract
Epithelial ovarian cancer is responsible for almost half of all the deaths from female genital tract tumors. Major impediments to the clinical treatment of this disease are the relatively asymptomatic progression and a lack of knowledge regarding defined precursor or malignant lesions. Most epithelial ovarian cancers are thought to arise from the transformation of ovarian surface epithelial cells, a single continuous layer of flat-to-cuboidal mesothelial cells surrounding the ovary. To improve our understanding of the pathogenesis of epithelial ovarian cancer, it is necessary to study the biological characteristics of normal ovarian surface epithelial cells. However, this approach has been hampered by the inability to purify and culture such human cells. During the past decade, procedures to isolate and culture human ovarian surface epithelial cells have been developed, and, subsequently, using viral oncogenes, several immortalized cells have been established. This new experimental system is being employed to improve our understanding of the genetic changes leading to the initiation of epithelial ovarian cancer and to identify events in the cancer's development. This review mainly describes the biological dynamics of ovarian surface epithelial cells in the pathogenesis of epithelial ovarian cancer, focusing on humans and excluding small animal models.
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- 2004
254. Cell biology of human ovarian surface epithelial cells and ovarian carcinogenesis
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Hidetaka Katabuchi and Hitoshi Okamura
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Ovulation ,Stromal cell ,media_common.quotation_subject ,Cellular differentiation ,Ovary ,Receptors, Cell Surface ,Biology ,Models, Biological ,Mice ,medicine ,Animals ,Humans ,Cells, Cultured ,media_common ,Cuboidal Cell ,Ovarian Neoplasms ,Cell Differentiation ,Epithelial Cells ,Epithelium ,Hormones ,Cell biology ,Microscopy, Electron ,medicine.anatomical_structure ,Cell Transformation, Neoplastic ,Microscopy, Electron, Scanning ,Neoplastic cell ,Female ,Anatomy ,Immortalised cell line - Abstract
Most epithelial ovarian carcinomas have been suggested to arise from the ovarian surface epithelium, which covers an ovary as a layer of flat to cuboidal cells. The epithelium is physiologically involved in follicular rupture and the subsequent repair of the follicle wall during reproductive age. Invagination and inclusion cysts are formed in the cortical stroma after cyclic ovulation. Consequently, ovulation may cause a loss of integrity of the surface epithelium followed by stepwise sequence of genetic alteration. Inclusion cysts are actually more common in ovaries contralateral to those containing malignant epithelial tumors than in control ovaries. Human ovarian surface epithelial cells exhibit a gland formation in coculture with endometrial stromal cells in an estrogen-rich environment. The phenotypic plasticity of these cells shares a mesenchymal property when they are cultured on two layers of extracellular matrix and collagen gel. As an in vitro study of ovarian carcinogensis, several neoplastic cell lines were recently established from the surface epithelial cells of the human ovary. SV 40 large T-antigen transfection into the epithelial cells induced some immortalized cell lines, one of which showed anchorage-independent growth and tumor formation in athymic mice. The tumors were histologically undifferentiated carcinoma. These cell lines may lead to insights into the preneoplastic and early stages of epithelial ovarian carcinomas. To understand the pathogenesis of epithelial ovarian cancer, specifically designed studies of ovarian surface epithelium and the related structural changes encountered after ovulation and these existing in ovarian carcinomas are required.
- Published
- 2003
255. Serum endostatin levels in patients with epithelial ovarian cancer
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Kohkichi, Hata, Dipok Kumar, Dhar, Haruhiko, Kanasaki, Kentaro, Nakayama, Ristuto, Fujiwaki, Hidetaka, Katabuchi, Hitoshi, Okamura, Naofumi, Nagasue, and Kohji, Miyazaki
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Adult ,Aged, 80 and over ,Ovarian Neoplasms ,Age Factors ,Epithelial Cells ,Middle Aged ,Peptide Fragments ,Collagen Type XVIII ,Endostatins ,Humans ,Female ,Collagen ,Aged ,Neoplasm Staging - Abstract
Endostatin, a 20-kDa C-terminal fragment of collagen XVIII, specifically inhibits endothelial proliferation and potently inhibits angiogenesis and tumor growth. The aim of this study was to assess the prognostic potentials of serum endostatin levels in epithelial ovarian cancer.The preoperative serum levels of endostatin in 61 cases of epithelial ovarian cancer (29 serous, 13 mucinous, 13 endometrioid, 5 clear cell and 1 Brenner cell) were analyzed using a competitive enzyme immunoassay. With regard to staging, 23 cases had stage I disease, 6 had stage II disease, 28 had stage III disease and 4 had stage IV disease.Serum levels were compared with levels from 22 age-matched healthy volunteer blood donors. The median serum levels were 18.5 ng/ml (range, 6.3-50.3 ng/ml) in patients with epithelial ovarian cancer and 18.4 ng/ml (range, 8.4-27.0 ng/ml) in controls. No significant difference was noted between the two groups. No clinicopathological features (e.g., patients' age at diagnosis, stage of disease, histological subtype and grade) were significantly associated with serum endostatin levels. Survival data were available for all patients. Multivariate Cox regression analysis revealed that FIGO stage III-IV (p = 0.015) and serum endostatin levels (2 standard deviations above the control mean; 27.7 ng/ml) (p = 0.035) are independent prognostic factors.Elevated serum levels of this endogenous inhibitor of angiogenesis may be a pertinent prognostic indicator for patients with epithelial ovarian cancer. It is anticipated that this finding may aid the development of a new therapeutic strategy for epithelial ovarian cancer.
- Published
- 2003
256. Necrotic feature of the trophoblasts lacking HLA-G expression in normal and pre-eclamptic placentas
- Author
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Noriko, Sageshima, Akiko, Ishitani, Motoko, Omura, Masayoshi, Akasaki, Hideshi, Umekage, Hidetaka, Katabuchi, Hitoshi, Okamura, and Katsuhiko, Hatake
- Subjects
HLA-G Antigens ,Placenta ,Pregnancy Trimester, Third ,Histocompatibility Antigens Class I ,Immunohistochemistry ,Trophoblasts ,Microscopy, Electron ,Necrosis ,Pregnancy Trimester, First ,Pre-Eclampsia ,HLA Antigens ,Pregnancy ,Pregnancy Trimester, Second ,Tumor Cells, Cultured ,Humans ,Female ,K562 Cells - Abstract
Human leukocyte antigen (HLA)-G is thought to be expressed in all placental extravillous trophoblasts (EXTs). In pre-eclamptic placentas, a lack of HLA-G expression on EXTs had been found, and deduced as a possible cause of pre-eclampsia. However, a subset of EXTs lacking expression of HLA-G can also be found in normal placenta. Therefore, we sought to compare these cells in normal and pre-eclamptic placentas.Frozen sections of normal and pre-eclamptic placentas were examined by immunohistochemical staining using HLA-G monoclonal antibody 87G, histochemical enzymatic analysis of succinate dehydrogenase (SDH) and ultrastructural analysis.A subset of EXTs lacking HLA-G expression was found in both normal and pre-eclamptic placentas. These cells showed necrotic features such as the swelling of cells, eosin-achromatophilia, the loss of SDH activity and swelling mitochondria. Cells from both tissues were identical with regard to these features.The features of the EXTs lacking HLA-G expression indicated they had undergone necrosis and thus could not express HLA-G protein. Therefore, an alternative interpretation to the lack of HLA-G expression in pre-eclamptic placentas is that it is the result of cell death and not the cause.
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- 2003
257. What we have learned from isolated cells from human ovary?
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Hitoshi Okamura, Takashi Ohba, and Hidetaka Katabuchi
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endocrine system ,medicine.medical_specialty ,Stromal cell ,Granulosa cell ,Cell Culture Techniques ,Endometriosis ,Gene Expression ,Ovary ,Cell Separation ,Biology ,Biochemistry ,Models, Biological ,Cell Line ,Mice ,Endocrinology ,Internal medicine ,medicine ,Animals ,Humans ,Molecular Biology ,Ovarian Neoplasms ,Granulosa Cells ,Estradiol ,Epithelial Cells ,Receptors, LH ,Coelomic epithelium ,Estradiol secretion ,Cell biology ,medicine.anatomical_structure ,Cell culture ,Receptors, FSH ,Female ,Clone (B-cell biology) ,Immortalised cell line - Abstract
In the ovary, morphodynamics of follicles with cyclic maturation, ovulation and repair occur under the control of various tropic factors. The ovarian functions have been mostly studied by using subhuman primates and non-primate animals because of the limited availability of closely staged human specimens. We have recently established the in vitro culture systems of ovarian surface epithelium (OSE) and granulosa cells of humans, and subsequently developed the immortalization of each cell. The immortalized cell lines may supply us advanced studies on ovarian disorders as well as its physiological functions. On the embryologically putative mullerian potential of coelomic epithelium, endometriosis can be explained by coelomic metaplasia from the peritoneal mesothelium, including OSE. We can microscopically observe a continuity from flat epithelial cells on the ovarian surface or within the cortical inclusion cysts to endometriotic gland cells. The primary human OSE cells exhibited a glandular-stromal structure similar to endometriosis when they were co-cultured with endometrial stromal cells in an estrogen-rich environment. Primary and immortalized OSE cells converted the estrone to estradiol, and expressed the genes for steroidogenic factor-1 (SF-1), p450arom and 17beta-HSDs. This character of OSE was, in part, similar to the granulosa cells. One of the immortalized OSE clone produces disseminated tumors mimicking undifferentiated carcinomas in nude mice. Ovarian granulosa cells play a key role in the functional maturation of the entire follicle. The molecular pathways in granulosa cells responsible for the growth, differentiation, and nursing the oocyte are still largely unknown. Our immortalized human granulosa cell line, GC1a, obtained from developing follicles, showed no steroid hormone biosynthesis, and no detectable expression of the genes for StAR or cytochrome p450 enzymes due to the lack of SF-1. Transfected SF-1 elicited estradiol secretion in GC1a cells with concomitant expression of the genes encoding the proteins for gonadal steroidogenesis. The enzymatic activity of 17beta-HSD was also achieved by SF-1 transgene. These results indicate that SF-1 controls the gene expression required for steroidogenesis in the human developing follicle. Clinically, immortalized GC1a cells from human origin, with steroidogenic capacity, may serve as a feeder layer for in vitro oocyte maturation. Further investigations of our immortalized OSE and granulosa cells of humans will allow us to clarify whether they have a single progenitor cell.
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- 2003
258. Characterization of macrophages in the decidual atherotic spiral artery with special reference to the cytology of foam cells
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Kazuo Matsui, Hitoshi Okamura, Sayuri Yih, Takashi Ohba, Hidetaka Katabuchi, Kiyoshi Takahashi, and Motohiro Takeya
- Subjects
Spiral artery ,Pathology ,medicine.medical_specialty ,Arteriosclerosis ,Placenta ,Pregnancy Complications, Cardiovascular ,Biology ,Fibrin ,Monocytes ,Pregnancy ,Lipid droplet ,Biopsy ,medicine ,Humans ,Fibrinoid necrosis ,Chemokine CCL2 ,medicine.diagnostic_test ,Monocyte ,Macrophages ,Smooth muscle layer ,Arteries ,medicine.disease ,Immunohistochemistry ,Microscopy, Electron ,medicine.anatomical_structure ,biology.protein ,Female ,Anatomy ,Infiltration (medical) ,Foam Cells - Abstract
“Acute atherosis” is characteristic in the spiral arteries of the placental bed of preeclampsia and a wide range of pregnancy disorders. The arterial lesion is histologically characterized by fibrinoid necrosis of the vessel walls with infiltration of foam cells, which under a light microscope appears similar to that seen in atherosclerosis. Although acute atherosis is currently considered as atheromatous-like lesions, the precise cellular mechanisms inducing these changes remain unelucidated. By histochemistry, immunohistochemistry, and electron microscopy, we investigated the decidual spiral arteries obtained by placental bed biopsy from 11 preeclamptic and 15 nonpreeclamptic women. In the decidual spiral arteries of preeclamptic patients, acute atherosis was observed in 23.5% (20/85 arteries). Fibrin deposition and accumulation of foam cells were observed more frequently in preeclamptic patients than in nonpreeclamptic patients. Endothelial cells remained in the atheromatous lesion, while the smooth muscle layer surrounding fibrin and foam cells became thin and was finally destroyed. The foam cells were immunohistochemically shown to be macrophages and neutral fat and phospholipids were histochemically demonstrated in them. Ultrastructurally, their cytoplasm was occupied by variously sized lipid droplets and membrane-bound myelin-like granules (myelinosomes). Plasma concentration of monocyte chemoattractant protein 1 (MCP-1), a potent monocyte chemoattractant factor, was significantly elevated in preeclamptic patients compared with normal healthy controls (P ≪ 0.01). In conclusion, injuries to the smooth muscle layer and intramural fibrinoid necrosis may result in infiltration of monocytes into the arterial walls, their maturation into macrophages, and the transformation into foam cells. Considering that atherosclerosis is developed by accumulation of lipid-laden macrophages and migration and proliferation of smooth muscle cells, the roles of macrophages in acute atherosis differ from those in atherosclerosis.
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- 2003
259. Hypoxia-inducible factor 1 alpha (HIF-1 alpha) gene expression in human ovarian carcinoma
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Kohkichi Hata, Atsuko Kanzaki, Hidetaka Katabuchi, Yuji Takebayashi, Kohji Miyazaki, Hitoshi Okamura, Manabu Fukumoto, and Kentaro Nakayama
- Subjects
Adult ,Vascular Endothelial Growth Factor A ,Cancer Research ,medicine.medical_specialty ,Time Factors ,Angiogenesis ,Antigens, CD34 ,Endothelial Growth Factors ,Biology ,Hypoxia-Inducible Factor 1-Alpha ,Downregulation and upregulation ,Internal medicine ,Ovarian carcinoma ,Gene expression ,medicine ,Humans ,RNA, Messenger ,Gene ,Survival analysis ,Aged ,Aged, 80 and over ,Ovarian Neoplasms ,Lymphokines ,Neovascularization, Pathologic ,Reverse Transcriptase Polymerase Chain Reaction ,Vascular Endothelial Growth Factors ,Carcinoma ,Middle Aged ,Hypoxia-Inducible Factor 1, alpha Subunit ,Immunohistochemistry ,Platelet Endothelial Cell Adhesion Molecule-1 ,Endocrinology ,Oncology ,Cancer research ,Female ,Endothelium, Vascular ,Immunostaining ,Transcription Factors - Abstract
Hypoxia-inducible factor 1 alpha (HIF-1 alpha) that regulates genes involved in response to hypoxia and promotes neo-angiogenesis, is a transcriptional factor for vascular endothelial cell growth factor (VEGF). The aim of this study was to examine the expression of HIF-1 alpha and VEGF gene expressions and their relation to angiogenesis, clinicopathologic variables and survival in the patient with human ovarian carcinoma. We retrospectively analyzed HIF-1 alpha and VEGF gene expression levels using reverse transcriptase polymerase chain reaction (RT-PCR) in 60 ovarian carcinomas. Intratumoral microvessel density (IMD) was assessed by immunostaining endothelial cells, using anti-CD 31 antibody in frozen sections. The relationships between the expression level of these genes, IMD and clinicopathologic variables were evaluated by Student's t-test and chi-square tests. Survival analysis was performed by Kaplan-Meier curves. HIF-1 alpha or VEGF gene expression level was independent of age, clinical stage and histological subtype besides grade of tumor. There was no relationship between HIF-1 alpha or VEGF gene expression level and IMD in all carcinomas (R=0.118 and 0.224, respectively). In addition, a weak association between HIF-1 alpha and VEGF gene expression level was observed (R=0.300, P=0.020). The association between VEGF gene expression and IMD was observed (R=0.501, P=0.016). However, no association between IMD and HIF-1 alpha gene expression was observed. Further, both HIF-1 alpha and VEGF gene expression levels had no effect on survival in the patient with ovarian carcinoma. These results suggest that VEGF upregulated by HIF-1 alpha gene may be involved in angiogenesis of some type of ovarian carcinoma, but the expression levels of both genes have no effect on survival in the patients with ovarian carcinoma.
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- 2002
260. The mystery of Hofbauer cells
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Hidetaka Katabuchi
- Subjects
Reproductive Medicine ,Obstetrics and Gynecology ,Biology ,Developmental Biology - Published
- 2014
261. Clinical significance of CD169-positive lymph node macrophages in human malignant tumors
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Motohiro Takeya, Yuji Miyamoto, Hideo Baba, Hironobu Ihn, Munekage Yamaguchi, Koji Ohnishi, Yoshihiro Komohara, Satoshi Fukushima, Hidetaka Katabuchi, and Yoichi Saito
- Subjects
Cancer Research ,Pathology ,medicine.medical_specialty ,business.industry ,Subcapsular Sinus ,Sialic acid ,chemistry.chemical_compound ,Oncology ,chemistry ,Sialoadhesin ,Positive lymph node ,Macrophage ,Medicine ,Clinical significance ,Animal studies ,Receptor ,business - Abstract
11118 Background: CD169 (sialoadhesin) is a sialic acid receptor that is expressed on specific macrophages such as lymph node sinus macrophages. Animal studies have suggested that CD169+ macrophage...
- Published
- 2014
262. Clinical value of thymidine kinase in patients with cervical carcinoma
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Hitoshi Okamura, Ritsuto Fujiwaki, Hidetaka Katabuchi, Kohji Miyazaki, Kohkichi Hata, Osamu Iwanari, and Masashi Moriyama
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Adult ,Cancer Research ,medicine.medical_specialty ,Uterine Cervical Neoplasms ,Cervix Uteri ,Thymidylate synthase ,Thymidine Kinase ,Gene Expression Regulation, Enzymologic ,Predictive Value of Tests ,Internal medicine ,Carcinoma ,medicine ,Biomarkers, Tumor ,Humans ,Neoplasm Invasiveness ,RNA, Messenger ,RNA, Neoplasm ,Thymidine phosphorylase ,Aged ,Thymidine Phosphorylase ,DNA synthesis ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Carcinoma in situ ,General Medicine ,Thymidylate Synthase ,Middle Aged ,medicine.disease ,Survival Analysis ,Carcinoembryonic Antigen ,Up-Regulation ,Reverse transcription polymerase chain reaction ,Gene Expression Regulation, Neoplastic ,Real-time polymerase chain reaction ,Endocrinology ,Oncology ,Thymidine kinase ,Multivariate Analysis ,Cancer research ,biology.protein ,Carcinoma, Squamous Cell ,Female ,Carcinoma in Situ - Abstract
Objective: Our purpose was to determine the clinical value of thymidine kinase (TK), which is an important pyrimidine pathway enzyme involved in salvage DNA synthesis, in patients with cervical carcinoma. Methods: We examined TK mRNA expression by reverse transcription polymerase chain reaction in 19 tissue specimens of invasive cervical carcinoma and 9 normal cervices and related it to thymidylate synthase (TS) and thymidine phosphorylase (TP) mRNA expressions. Serum TK level was determined by radioenzymatic assay in 79 patients with invasive cervical carcinoma, 7 patients with microinvasive carcinoma, 21 patients with carcinoma in situ and 32 normal women. Results: TK mRNA expression was upregulated in invasive cervical carcinoma compared with the normal cervix (p < 0.05) and significantly correlated with TS mRNA expression (p < 0.0001) but not with TP mRNA expression. The serum TK level was significantly higher in patients with invasive carcinoma than in normal women and patients with carcinoma in situ (p < 0.01 and p < 0.05). In patients with invasive cervical carcinoma, the serum TK level significantly correlated with TK mRNA expression (p < 0.05), but not with any conventional clinicopathologic factors. High serum TK levels significantly correlated with a poorer survival (p < 0.05), and multivariate analysis showed serum TK level to be an independent prognostic factor (p < 0.05). Conclusion: TK may play an important role in influencing the malignant behavior of cervical carcinoma, and measurement of the serum TK level may be useful in predicting survival in patients with cervical carcinoma.
- Published
- 2001
263. Characterization and tumorigenicity of human ovarian surface epithelial cells immortalized by SV40 large T antigen
- Author
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Hideyuki Ohtake, Hitoshi Okamura, Hironori Tashiro, Masaru Yamaizumi, Hidetaka Katabuchi, and Makoto Nitta
- Subjects
endocrine system ,Pathology ,medicine.medical_specialty ,Antigens, Polyomavirus Transforming ,Immunocytochemistry ,Transplantation, Heterologous ,Mice, Nude ,Simian virus 40 ,Biology ,Transfection ,Cytokeratin ,Mice ,Antigen ,medicine ,Animals ,Humans ,Neoplastic transformation ,Aged ,Cell Line, Transformed ,Ovarian Neoplasms ,Mice, Inbred BALB C ,Ovary ,Obstetrics and Gynecology ,Epithelial Cells ,Middle Aged ,medicine.disease ,Cell Transformation, Viral ,Epithelium ,Mesothelium ,medicine.anatomical_structure ,Oncology ,Cell culture ,Cancer research ,Female ,Ovarian cancer ,Cell Division ,Neoplasm Transplantation - Abstract
Objectives. Epithelial ovarian cancers are considered to arise from neoplastic transformation of the ovarian surface epithelium (OSE). However, the earliest events in ovarian carcinogenesis have not been clearly defined because patients are often diagnosed in the advanced stages and useful in vivo and in vitro experimental systems using human OSE cells are lacking. We aimed to improve the availability of experimental models for the study of human ovarian carcinogenesis. Methods. Subcultured human OSE cells were transfected with SV40 large T antigen. Resulting OSE cell lines were characterized using immunocytochemistry and tested tumorigenicity. Results. Six immortalized OSE cell lines were obtained. All cell lines essentially retained the original morphological features of normal OSE cells and showed higher proliferation rates and saturation density. Although they were all nontumorigenic in athymic mice, OSE2b-2 sv cells, which were selected in soft agar from colonies of an SV40 large T antigen-expressing transfectant, OSE2b sv, produced tumors on the peritoneal surface, mesothelium, and diaphragm and induced ascites after being injected intraperitoneally. Solid tumors also grew when mice were inoculated subcutaneously. The tumor cells were formed in a solid sheet arrangement and no evidence of glandular or squamous differentiation was present. They were weakly immunostained with an antibody against cytokeratin, and intercellular junctions resembling attachment devices were ultrastructurally present between cells. The tumors were histologically diagnosed as undifferentiated carcinomas. Conclusions. The established cell lines may provide a model system to investigate the mechanisms of cytogenic and molecular changes from normal OSE cells through the various steps of transformation.
- Published
- 2001
264. Human villous macrophage-conditioned media enhance human trophoblast growth and differentiation in vitro
- Author
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Salma Khan, Hidetaka Katabuchi, Hitoshi Okamura, Ryuichiro Nishimura, and Masako Araki
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Macrophage colony-stimulating factor ,Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,Radioimmunoassay ,Enzyme-Linked Immunosorbent Assay ,Endothelial Growth Factors ,Biology ,Chorionic Gonadotropin ,Human placental lactogen ,Pregnancy ,Internal medicine ,medicine ,Macrophage ,Humans ,reproductive and urinary physiology ,Chemokine CCL2 ,DNA Primers ,Lymphokines ,Cytotrophoblast ,Reverse Transcriptase Polymerase Chain Reaction ,Vascular Endothelial Growth Factors ,Monocyte ,Macrophage Colony-Stimulating Factor ,Macrophages ,Lymphokine ,Trophoblast ,Cell Differentiation ,Cell Biology ,General Medicine ,Chorion ,Fibroblasts ,Placental Lactogen ,Molecular biology ,Immunohistochemistry ,Trophoblasts ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,Culture Media, Conditioned ,embryonic structures ,Hofbauer cell ,Macrophages, Peritoneal ,Female - Abstract
In human chorionic villi, numerous macrophages, so-called Hofbauer cells, are located adjacent to trophoblasts. To determine the role of the macrophages in the proliferation and differentiation of trophoblasts, cytotrophoblast cells were cultured in serum-free culture-conditioned media of villous macrophages (VMCM), peritoneal macrophages (PMCM), and villous fibroblasts (VFCM). In VMCM, proliferation of cytotrophoblast cells was detected at 24 h by immunocytochemistry with Ki-67-antibody. A large number (P < 0.001) of multinucleated syncytia was formed in VMCM. In VMCM, cytotrophoblast cell fusion was completed by 96 h, which coincided with the peak of hCG secretion and initiation of human placental lactogen (hPL) release. Levels of hCG (P < 0.001) and hPL (P < 0. 001) secretion from syncytial cells were significantly higher in VMCM than in PMCM or in VFCM. Concentrations of macrophage colony-stimulating factor (M-CSF) and vascular endothelial growth factor (VEGF) analyzed by ELISA were greater in VMCM than in PMCM or in VFCM, whereas monocyte chemoattractant protein-1 (MCP-1) concentration was high in PMCM. The expression patterns of M-CSF, VEGF, and MCP-1 in villous macrophages and peritoneal macrophages by reverse transcriptase-polymerase chain reaction were similar to their secretion patterns. Thus, villous macrophages have a greater ability to stimulate hCG and hPL secretion than do peritoneal macrophages. This study suggests that macrophages within the villous stroma may stimulate the growth and differentiation of trophoblasts through their secreted substances.
- Published
- 2000
265. The molar vesicle fluid contains the beta-core fragment of human chorionic gonadotropin
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Salma Khan, Takashi Ohba, R. Nishimura, Hidetaka Katabuchi, Hitoshi Okamura, T. Koizumi, and Masako Araki
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Molar ,endocrine system ,medicine.medical_specialty ,In Vitro Techniques ,Chorionic Gonadotropin ,Chromatography, Affinity ,Human chorionic gonadotropin ,Stroma ,Fetal membrane ,Pregnancy ,Internal medicine ,Placenta ,medicine ,Humans ,Chorionic Gonadotropin, beta Subunit, Human ,reproductive and urinary physiology ,biology ,Vesicle ,Obstetrics and Gynecology ,Hydatidiform Mole ,Immunohistochemistry ,Peptide Fragments ,Body Fluids ,Cytosol ,Endocrinology ,medicine.anatomical_structure ,Reproductive Medicine ,Uterine Neoplasms ,biology.protein ,Macrophages, Peritoneal ,Female ,Antibody ,Developmental Biology - Abstract
The human chorionic gonadotropin (hCG) beta-core fragment (beta-CF) is a major molecular form of hCG beta subunit (hCGbeta) immunoreactivity in the urine of pregnant women and patients with trophoblastic disease. The majority of evidence supports the fact that the beta-CF is a degradative product of intact hCG and free hCGbeta in the kidneys. We found a beta-CF-like substance in the fluid of molar vesicles from a patient with complete hydatidiform mole. The molar fluid beta-CF (mbeta-CF) was indistinguishable from the beta-CF in the patient's urine (ubeta-CF) by immunoreactivity and by elution profile on gel chromatography. The binding study to lectins, however, showed that mbeta-CF contains a carbohydrate moiety that differs from that of ubeta-CF. Immunohistochemistry with anti-beta-CF antibody demonstrated a strong immunoreactivity in a large number of macrophages in the molar villous stroma. In vitro incubation of intact hCG with peritoneal macrophages showed a slow increase of intact hCG in the cell cytosol with the appearance of beta-CF-like substance in the cell supernatant. In conclusion, the source of beta-CF in molar fluid is likely to be macrophages existing in the villous stroma. Thus macrophages may ingest intact hCG and act as a local regulator of gonadotropic hormones.
- Published
- 2000
266. Predicting the effect of gonadotropin-releasing hormone (GnRH) analogue treatment on uterine leiomyomas based on MR imaging
- Author
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Y. Kitano, Yasuyuki Yamashita, Hidetaka Katabuchi, T. Shimamura, M. Takahashi, Yasuji Matsuno, and Hitoshi Okamura
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Adult ,Pathology ,medicine.medical_specialty ,Antineoplastic Agents, Hormonal ,medicine.medical_treatment ,Uterus ,Gonadotropin-releasing hormone ,Buserelin ,Predictive Value of Tests ,Leiomyomatosis ,Administration, Inhalation ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,neoplasms ,Hysterectomy ,Uterine leiomyoma ,Radiological and Ultrasound Technology ,medicine.diagnostic_test ,business.industry ,Magnetic resonance imaging ,General Medicine ,Middle Aged ,musculoskeletal system ,medicine.disease ,Magnetic Resonance Imaging ,female genital diseases and pregnancy complications ,body regions ,surgical procedures, operative ,medicine.anatomical_structure ,Leiomyoma ,Treatment Outcome ,In utero ,Uterine Neoplasms ,Female ,business ,medicine.drug - Abstract
Purpose: To test the hypothesis that the simple assessment of signal intensity on T2-weighted MR images is predictive of the effect of hormonal treatment with gonadotropin-releasing hormone (GnRH) analogue. Material and Methods: The correlation between T2-weighted MR imaging of uterine leiomyomas and histologic findings was evaluated using 85 leiomyomas from 62 females who underwent myomectomy or hysterectomy. We also correlated the pretreatment MR images features obtained in 110 women with 143 leiomyomas with the effect of GnRH analogue treatment. The size (length×width×depth) of the leiomyoma was evaluated before and at 6 months after treatment by ultrasound. Results: The proportion of leiomyoma cell fascicles and that of extracellular matrix affected signal intensities of uterine leiomyomas on T2-weighted MR images. The amount of extracellular matrix was predominant in hypointense leiomyomas on T2-weighted images, while diffuse intermediate signal leiomyomas were predominantly composed of leiomyoma cell fascicles. Marked degenerative changes were noted in leiomyomas with heterogenous hyperintensity. The homogeneously intermediate signal intensity leiomyomas showed significant size reduction after treatment (size ratio; posttreatment volume/pretreatment volume 0.29±0.11). The size ratio for the hypointense tumors was 0.82±0.14, and 0.82±0.18 for the heterogeneously hyperintense tumors. There was a significant difference in the response to treatment between the homogeneously intermediate signal intensity leiomyomas and the hypointense or heterogeneously hyperintense leiomyomas (both p Conclusion: Signal intensity on T2-weighted MR images depends on the amount of leiomyoma cell fascicles and extracellular matrix. Simple assessment of the MR signal intensity is useful in predicting the effect of GnRH analogue on uterine leiomyomas.
- Published
- 1999
267. A follicle-stimulating hormone-secreting gonadotroph adenoma with ovarian enlargement in a 10-year-old girl
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Hironori Tashiro, Yukitaka Ushio, Hidetaka Katabuchi, Hideyuki Ohtake, Hitoshi Okamura, and Takayuki Kaku
- Subjects
Adenoma ,endocrine system ,Pathology ,medicine.medical_specialty ,endocrine system diseases ,medicine.drug_class ,medicine.medical_treatment ,Biology ,Monoclonal antibody ,Follicle-stimulating hormone ,Internal medicine ,medicine ,Tumor Cells, Cultured ,Humans ,Cyst ,Pituitary Neoplasms ,Child ,Transsphenoidal surgery ,Obstetrics and Gynecology ,Luteinizing Hormone ,medicine.disease ,Polycystic ovary ,Magnetic Resonance Imaging ,Ovarian Cysts ,Endocrinology ,Reproductive Medicine ,Culture Media, Conditioned ,Immunohistochemistry ,Female ,Gonadotropin ,Follicle Stimulating Hormone ,hormones, hormone substitutes, and hormone antagonists - Abstract
Objective: To report a rare case of gonadotroph adenoma accompanied by ovarian enlargement in a child. Design: Case report. Setting: A university hospital. Patient(s): A 10-year-old Japanese girl with multiple cysts of both ovaries. Intervention(s): Endocrinologic assays, immunohistochemical staining, ultrastructural observations, and in vitro analysis. Main Outcome Measure(s): The endocrinologic assays showed an elevated serum FSH level. The tumor cells excised from the gonadotroph adenoma were immunohistochemically positive for antihuman FSH monoclonal antibody. When cultured in vitro, the tumor cells secreted FSH in the primary culture. Result(s): The gonadotroph adenoma produced FSH. After transsphenoidal surgery, both ovaries decreased in size. Conclusion(s): The ovarian enlargement was induced by endogenous FSH from the gonadotroph adenoma. To our knowledge, this is the first reported case of gonadotroph adenoma accompanied by ovarian enlargement in childhood or adolescence.
- Published
- 1999
268. A novel in vitro experimental model for ovarian endometriosis: the three-dimensional culture of human ovarian surface epithelial cells in collagen gels
- Author
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Kohei Matsuura, Hitoshi Okamura, Hidetaka Katabuchi, and Hideyuki Ohtake
- Subjects
Adult ,endocrine system ,Stromal cell ,Cell ,Endometriosis ,Ovary ,Biology ,Endometrium ,Cytokeratin ,medicine ,Humans ,Cells, Cultured ,Aged ,Estradiol ,Obstetrics and Gynecology ,Epithelial Cells ,Middle Aged ,Embryonic stem cell ,Molecular biology ,Immunohistochemistry ,Epithelium ,Coculture Techniques ,Microscopy, Electron ,medicine.anatomical_structure ,Reproductive Medicine ,Immunology ,Ovarian Endometriosis ,Female ,Collagen - Abstract
Objective: To develop an in vitro experimental model of ovarian endometriosis using human cells and to investigate the pathogenesis of endometriosis. Design: Controlled in vitro coculture study. Setting: A department of obstetrics and gynecology at a university hospital. Patient(s): Ovaries and endometrium were obtained from patients who underwent a hysterectomy because of gynecologic disease. Intervention(s): Human ovarian surface epithelial (OSE) cells were cultured alone and OSE cells and endometrial stromal (ES) cells were cultured together in a three-dimensional collagen gel culture system with or without the addition of E 2 . Main Outcome Measure(s): The aggregated collagen gels containing the cultured cells were examined morphologically. Result(s): The OSE cells in single culture with E 2 formed circular arrangements. These cells were immunohistochemically positive for cytokeratin but negative for epithelial membrane antigen. In the cocultures of OSE and ES cells with E 2 , the OSE cells formed a lumen structure surrounded by ES cells. Immunoreactivity for cytokeratin and epithelial membrane antigen was detected in the glandular cells and cilia were identified on the cell surface by electron microscopy. Without the addition of E 2 , no structures were detected. Conclusion(s): A new in vitro experimental model was established with the aid of human OSE cells. Endometriotic lesions can arise through a process of metaplasia from OSE cells in the presence of E 2 and ES cells.
- Published
- 1999
269. Increased serum levels of 5-S-cysteinyldopa and intercellular adhesion molecule-1 in a patient with a uterine amelanotic metastasis from a primary vaginal malignant melanoma
- Author
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Hitoshi Okamura, Toshiro Kageshita, Tomomichi Ono, Hidetaka Katabuchi, and Yoshito Suenaga
- Subjects
Pathology ,medicine.medical_specialty ,Vaginal Neoplasms ,business.industry ,Melanoma ,Intercellular Adhesion Molecule-1 ,Uterus ,Obstetrics and Gynecology ,medicine.disease ,Metastasis ,Cysteinyldopa ,medicine.anatomical_structure ,Vaginal disease ,Oncology ,Uterine Neoplasms ,medicine ,Vagina ,Humans ,Enlarged Uterus ,Female ,business ,Lymph node ,Aged - Abstract
A 75-year-old Japanese woman, who had an enlarged uterus and two nodules on the anterior vaginal wall, underwent wide excision of the anterior vaginal wall, bilateral inguinal lymphadenectomy, modified radical hysterectomy, and pelvic lymph node dissection. Her serum level of 5-S-cysteinyldopa (5-S-CD) was elevated, but her serum intercellular adhesion molecule-1 (ICAM-1) level was within normal limits. Histologic examination of the vaginal wall revealed atypical melanocytes at the dermoepidermal junction and numerous melanin-containing granules in the cytoplasm of the tumor cells. The tumor in the uterus consisted of sheets of anaplastic cells with evidence of diminished melanization. Five months after her initial presentation, she developed a recurrence and expired. The serum level of ICAM-1 was beyond the normal limit at the time of recurrence. Both the serum 5-S-CD and the ICAM-1 levels became elevated after recurrence and appeared to be correlated with disease progression.
- Published
- 1999
270. Distribution of 17β-hydroxysteroid dehydrogenase isozymes in normal and abnormal human endometrium
- Author
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Hironori Tashiro, Y. Taura, Takashi Ohba, Hidetaka Katabuchi, and Ken ichi Motohara
- Subjects
Reproductive Medicine ,Chemistry ,Obstetrics and Gynecology ,Distribution (pharmacology) ,Hydroxysteroid dehydrogenase ,Human endometrium ,Molecular biology ,Isozyme - Published
- 2008
271. Distribution and cytological properties of macrophages in human Fallopian tubes
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Hidetaka Katabuchi, Yoshito Suenaga, H Okamura, and Y. Fukumatsu
- Subjects
Adult ,Histology ,Antigens, Differentiation, Myelomonocytic ,Cell Count ,Vacuole ,Immunoenzyme Techniques ,Stroma ,Antigens, CD ,Organelle ,medicine ,Macrophage ,Humans ,Fallopian Tubes ,Menstrual Cycle ,Aged ,biology ,CD68 ,Monocyte ,Macrophages ,Antibodies, Monoclonal ,Muscle, Smooth ,Middle Aged ,Cell biology ,Postmenopause ,medicine.anatomical_structure ,Premenopause ,Cytoplasm ,Immunology ,biology.protein ,Female ,Anatomy ,Antibody - Abstract
The macrophages in human Fallopian tubes of women in the reproductive and postmenopausal periods were examined with a focus on their morphological properties by immunohistochemical staining and transmission electron microscopy. The fine structure of the smooth muscle cells in the Fallopian tubes was also investigated during the reproductive period. For immunohistochemical staining, we used two monoclonal antibodies that were specific for human macrophages, namely PM-1K and PM-2K. PM-1K recognizes human monocytes/ macrophages corresponding to CD68, and PM-2K recognizes tissue macrophages. PM-1K-positive cells were always present and their numbers increased significantly during the menstrual and early to mid-secretory phases. In contrast, relative numbers of PM-2K-positive cells were small throughout the menstrual cycle. In the postmenopausal period, few PM-1K-positive cells were detected, but PM-2K-positive cells remained. The macrophages during the secretory phase in the endosalpingeal stroma had well-developed intracytoplasmic organelles, but relatively few cytoplasmic vacuoles and granules. In the same phase, many cells of the monocyte/macrophage lineage appeared in the vascular lumen of the endosalpingeal stroma. The macrophages during the menstrual phase had well-developed intracellular organelles, with cytoplasmic vacuoles and granules of various sizes and configurations. During the late secretory phase, just prior to menstruation, the smooth muscle cells contained few cytoplasmic filaments but electron-lucent or electron-dense lysosome-like bodies were seen. These findings suggested the presence of macrophages in human Fallopian tubes. It is possible that such macrophages might be involved in the physiological functions of the tubes during the reproductive period and moreover that they might participate in the reconstruction of the muscle layer of the tubes.
- Published
- 1998
272. Establishment of a new cell line, OKT1, from small cell carcinoma secreting ectopic ACTH of the uterine cervix
- Author
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Hironori Tashiro, Y. Fukumatsu, Hideyuki Ohtake, Hidetaka Katabuchi, Salma Khan, and Hitoshi Okamura
- Subjects
Adult ,Pathology ,medicine.medical_specialty ,Cellular differentiation ,Transplantation, Heterologous ,Uterine Cervical Neoplasms ,Adrenocorticotropic hormone ,Gene mutation ,Biology ,Cell morphology ,Small-cell carcinoma ,Neuroendocrine differentiation ,Mice ,Adrenocorticotropic Hormone ,Carcinoma ,medicine ,Tumor Cells, Cultured ,Animals ,Humans ,Carcinoma, Small Cell ,Neuroendocrine cell ,Chromosome Aberrations ,Obstetrics and Gynecology ,medicine.disease ,Genes, p53 ,medicine.anatomical_structure ,Oncology ,Hormones, Ectopic ,Female ,Cell Division ,Neoplasm Transplantation - Abstract
Objective. Small cell carcinoma of the uterine cervix is rare and represents a unique entity among gynecological tumors. It sometimes demonstrates neuroendocrine differentiation, including adrenocorticotropin (ACTH) secretion. In this study, we established a new cell line, OKT1, from a case of carcinoma secreting ectopic ACTH without Cushing's syndrome and determined the character of the cell line. Methods. OKT1 was established from OKT tumor cells, derived from a biopsy specimen of small cell cervical carcinoma, and serially heterotransplanted into nude mice. To characterize OKT1, the cell morphology, growth properties, immunohistochemical properties, hormone- and tumor-associated antigen secretion, tumorigenic potential, DNA profile, and chromosomal alteration were studied. Results. The population doubling time of OKT1 was approximately 27 h. The cytological properties of OKT1, including DNA ploidy pattern, were similar to those of the primary tumor. Neuroendocrine differentiation was shown in the OKT1 cells by the positive immunocytochemical staining of neuron-specific enolase (NSE) and the presence of NSE and ACTH in the culture media. The xenograft of 1 × 10 8 OKT1 cells into nude mice yielded tumor mass. Furthermore, OKT1 demonstrated HPV type 18 and absence of a p53 gene mutation from exons 5 through 8. Conclusion. To our knowledge, OKT1 is the first cell line established from small cell cervical carcinoma with ACTH secretion.
- Published
- 1998
273. Distribution and fine structure of macrophages in the human ovary during the menstrual cycle, pregnancy and menopause
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Hidetaka Katabuchi, Yoshito Suenaga, Hitoshi Okamura, and Y. Fukumatsu
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Adult ,endocrine system ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Population ,Ovary ,Biology ,Luteal phase ,Follicle ,Endocrinology ,Pregnancy ,Internal medicine ,medicine ,Humans ,Ovarian follicle ,education ,Menstrual Cycle ,Aged ,Atretic Follicle ,education.field_of_study ,Macrophages ,Middle Aged ,Corpus albicans ,Microscopy, Electron ,medicine.anatomical_structure ,Female ,Menopause ,Corpus luteum - Abstract
We evaluated the distribution and ultrastructural characteristics of macrophages in the ovaries of women of reproductive ages, during pregnancy, and after menopause, by immunohistochemistry and transmission electron microscopy. Macrophages appeared around the ovarian follicle with its development. Their organelles were poorly developed, and no vacuoles or granules were observed in the cytoplasm. Macrophages were also present in the cavity of the atretic follicle, being larger in size than those in the developing follicle and characterized by cytoplasmic vacuoles and granules of a lysosomal nature. With the luteinization of the follicle, macrophages were seen to be distributed inside and outside the corpus luteum, but constituted only a minor population as compared with other kinds of leukocytes. The intracellular organelles were well-developed, including the lysosomal granules. In early pregnancy, the number of macrophages was noticeably increased in the corpus luteum. They were observed mainly outside the corpus luteum, and stained strongly with hCG immunohistochemically. Macrophages were present in the regressing corpus luteum and in the corpus albicans. Numerous lipid droplets and elongated cholesterol crystals were seen in the cytoplasm. Macrophages therefore appeared to be present throughout the ovarian cycle and may be involved in the development and atresia of the follicles and the progression and the regression of luteal tissues.
- Published
- 1998
274. An Ovarian Carcinoid Tumor With Peptide YY-Positive Insular Component: A Case Report and Review of the Literature.
- Author
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Erdenebaatar, Chimeddulam, Munekage Yamaguchi, Fumitaka Saito, Chisato Motooka, Hironori Tashiro, and Hidetaka Katabuchi
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- 2016
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275. CD44 Variant 6 as a Predictive Biomarker for Distant Metastasis in Patients With Epithelial Ovarian Cancer.
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Takeshi Motohara, Koichi Fujimoto, Shingo Tayama, Dashdemberel Narantuya, Isao Sakaguchi, Hironori Tashiro, Hidetaka Katabuchi, Motohara, Takeshi, Fujimoto, Koichi, Tayama, Shingo, Narantuya, Dashdemberel, Sakaguchi, Isao, Tashiro, Hironori, and Katabuchi, Hidetaka
- Published
- 2016
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276. Adenoma malignum: MR imaging and pathologic study
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Takako Doi, Hidetaka Katabuchi, Hitoshi Okamura, Keizo Fujiyoshi, Yasunaga T, Arata Tsunawaki, Nobuyuki Tanaka, Yasuyuki Yamashita, and Mutsumasa Takahashi
- Subjects
Adult ,medicine.medical_specialty ,Pathology ,Biopsy ,Uterus ,Uterine Cervical Neoplasms ,Gadolinium ,Adenocarcinoma ,Diagnosis, Differential ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Cervix ,Retrospective Studies ,medicine.diagnostic_test ,business.industry ,Reproducibility of Results ,Magnetic resonance imaging ,Middle Aged ,medicine.disease ,Image Enhancement ,Magnetic Resonance Imaging ,medicine.anatomical_structure ,Uterine cervix ,Lobular Endocervical Glandular Hyperplasia ,Adenoma malignum ,Female ,Radiology ,business - Abstract
To evaluate the clinical, pathologic, and magnetic resonance (MR) imaging findings in adenoma malignum, a rare variant of uterine cervical adenocarcinoma.Medical records of all patients (n = 7) with adenoma malignum of the uterine cervix diagnosed pathologically between 1988 and 1996 were retrospectively reviewed. Unenhanced T1-weighted and T2-weighted images and gadolinium-enhanced T1-weighted MR images were evaluated, and findings were correlated with gross pathologic and microscopic features.In five of seven patients, enlargement of the cervix was seen. All lesions were detected as multiple cystic lesions that extended from the endocervical gland to the deep stroma of the cervix. They appeared isointense (n = 5) or slightly hyperintense (n = 2) relative to the uterus on T1-weighted images and markedly hyperintense relative to the uterus on T2-weighted images. Solid portions of variable size were seen between cystic lesions, and both the multiple cystic component and the solid portion were most apparent on the gadolinium-enhanced T1-weighted images. Microscopic parametrial invasion was seen in two patients but was not detected at MR imaging.Adenoma malignum was depicted on MR images as a multicystic mass with solid portions located in the deep cervical stroma. Gadolinium enhancement helped identify the solid portion of the tumor.
- Published
- 1997
277. Long-Term Outcome of Aromatase Inhibitor Therapy With Letrozole in Patients With Advanced Low-Grade Endometrial Stromal Sarcoma.
- Author
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Munekage Yamaguchi, Chimeddulam Erdenebaatar, Fumitaka Saito, Takeshi Motohara, Yo Miyahara, Hironori Tashiro, and Hidetaka Katabuchi
- Published
- 2015
- Full Text
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278. The clinical presentation of placental mesenchymal dysplasia; Japanese publication based study
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Midori Okajima, Isao Sakaguchi, Masaharu Fukunaga, Saori Aoki, Hidenobu Soejima, Hidetaka Katabuchi, Takashi Ohba, and Ken Higashimoto
- Subjects
Gynecology ,medicine.medical_specialty ,Pathology ,Reproductive Medicine ,business.industry ,medicine ,Obstetrics and Gynecology ,Presentation (obstetrics) ,business ,Placental Mesenchymal Dysplasia ,Developmental Biology - Published
- 2013
279. Molecular genetic analyses of 11p15 region in placentae with mesenchymal dysplasia
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Kosuke Jozaki, Hidetaka Katabuchi, Ken Higashimoto, Toshiyuki Maeda, Midori Okajima, Isao Sakaguchi, Saori Aoki, Yasufumi Ohtsuka, Hitomi Yatsuki, Takashi Ohba, and Hidenobu Soejima
- Subjects
Pathology ,medicine.medical_specialty ,Reproductive Medicine ,medicine ,Obstetrics and Gynecology ,Mesenchymal Dysplasia ,Biology ,Developmental Biology - Published
- 2013
280. Direct injection of methotrexate preserves fertility in patients with ectopic pregnancy in lower uterine segment
- Author
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M. Yamaguchi, Takashi Ohba, R. Honda, Hidetaka Katabuchi, K. Uchino, and Tomoko Honda
- Subjects
Gynecology ,medicine.medical_specialty ,Lower uterine segment ,Ectopic pregnancy ,Obstetrics ,business.industry ,media_common.quotation_subject ,Obstetrics and Gynecology ,Fertility ,medicine.disease ,Reproductive Medicine ,medicine ,Methotrexate ,In patient ,business ,media_common ,medicine.drug - Published
- 2013
281. Possible involvement of signal transducer and activator of transcription-3 in cell–cell interactions of peritoneal macrophages and endometrial stromal cells in human endometriosis
- Author
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Hironori Tashiro, Hidetaka Katabuchi, Motohiro Takeya, Kiyomi Takaishi, R. Honda, Fumiko Itoh, Yoshihiro Komohara, and Satoru Kyo
- Subjects
STAT3 Transcription Factor ,Pathology ,medicine.medical_specialty ,Stromal cell ,Cell ,Endometriosis ,Cell Communication ,Endometrium ,Cell Line ,medicine ,Humans ,STAT3 ,Cells, Cultured ,reproductive and urinary physiology ,Cell Proliferation ,biology ,urogenital system ,business.industry ,Peritoneal fluid ,Obstetrics and Gynecology ,medicine.disease ,Coculture Techniques ,medicine.anatomical_structure ,Reproductive Medicine ,embryonic structures ,Macrophages, Peritoneal ,STAT protein ,Cancer research ,biology.protein ,Female ,Stromal Cells ,business ,CD163 - Abstract
Objective To investigate interactions between peritoneal macrophages and endometrial stromal cells (ESCs) involved in the development of endometriosis. Design Clinicopathologic and in vitro studies. Setting Department of Obstetrics and Gynecology and Department of Pathology, Kumamoto University Hospital. Patient(s) Women undergoing laparoscopy or laparotomy to treat endometriosis or other benign gynecologic conditions. Intervention(s) We collected samples of peritoneal fluid (ascites), endometrium, and endometriotic tissues. We cocultured ESCs in vitro with or without human macrophages. Main Outcome Measure(s) Macrophage phenotypes in peritoneal fluid were determined via immunostaining. Proliferation of ESCs and activation of signal transducer and activator of transcription-3 (Stat3) in cocultures were evaluated. Result(s) The endometriosis group had a significantly higher total number of macrophages in ascites compared with the control group, but the ratios of CD163+ alternatively activated macrophages (M2) in the two groups did not differ significantly. Coculture with M2 macrophages significantly up-regulated ESC proliferation and Stat3 activation in ESCs in vitro. Proliferation of ESCs was suppressed after Stat3 was down-regulated by small interfering RNA. Stat3 was activated in epithelial cells and ESCs in human endometriotic lesions. Conclusion(s) Interactions between M2 macrophages and ESCs via Stat3 activation may play an important role in the development of endometriosis.
- Published
- 2013
282. Activated (HLA-DR+) T-lymphocyte subsets in early epithelial ovarian cancer and malignant ovarian germ cell tumors
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Futoshi Arakane, Hitoshi Okamura, Hidetaka Katabuchi, K. Shimada, Kohji Miyazaki, and S. Arao
- Subjects
CD4-Positive T-Lymphocytes ,Pathology ,medicine.medical_specialty ,Endometriosis ,Ovary ,CD8-Positive T-Lymphocytes ,medicine.disease_cause ,Lymphocyte Activation ,T-Lymphocyte Subsets ,medicine ,HLA-DR ,Humans ,Sex Cord-Gonadal Stromal Tumors ,Neoplasm Staging ,Ovarian Neoplasms ,Cell growth ,business.industry ,Obstetrics and Gynecology ,T lymphocyte ,HLA-DR Antigens ,medicine.disease ,medicine.anatomical_structure ,Oncology ,Female ,Germ cell tumors ,Germinoma ,Carcinogenesis ,business ,CD8 - Abstract
We examined peripheral blood T-lymphocyte subsets before initiation of therapy in 79 healthy controls, 3 patients with endometriosis, 95 patients with common epithelial tumors of the ovary, 15 patients with ovarian germ cell tumors, and 3 patients with ovarian sex cord-stromal tumors. In stage Ia/Ib patients with epithelial ovarian cancer, the percentages of activated CD4+ (CD4+HLA-DR+) T cells and activated CD4+ T cells in the CD4+ T-cell subsets were significantly higher than those of healthy controls and patients with benign or borderline epithelial tumors of the ovary. These immunologic parameters were subsequently decreased in patients in stage Ic and more advanced stages. In malignant ovarian germ cell tumors, a similar increase in the CD4+ T-cell subsets was observed. Moreover, the percentage of activated CD8+ T cells in the CD8+ T-cell subsets in stage Ia/Ib patients increased significantly compared with those in healthy controls and patients with benign tumors. Our findings indicate that activated T lymphocytes may play some roles in oncogenesis and progression of both epithelial ovarian cancer and malignant ovarian germ cell tumors.
- Published
- 1995
283. Activated (HLA-DR+) T-lymphocyte subsets in cervical carcinoma and effects of radiotherapy and immunotherapy with sizofiran on cell-mediated immunity and survival
- Author
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Kohji Miyazaki, Hidetaka Katabuchi, S. Fujisaki, Keizo Fukuma, Hitoshi Okamura, and Hiroshi Mizutani
- Subjects
CD4-Positive T-Lymphocytes ,Cellular immunity ,Sizofiran ,medicine.medical_treatment ,CD4-CD8 Ratio ,Uterine Cervical Neoplasms ,CD8-Positive T-Lymphocytes ,Lymphocyte Activation ,chemistry.chemical_compound ,T-Lymphocyte Subsets ,medicine ,Humans ,Lymphocyte Count ,Prospective Studies ,Survival rate ,Proportional Hazards Models ,Chemotherapy ,business.industry ,Obstetrics and Gynecology ,T lymphocyte ,Immunotherapy ,HLA-DR Antigens ,Middle Aged ,Combined Modality Therapy ,Radiation therapy ,Survival Rate ,Oncology ,chemistry ,Chemotherapy, Adjuvant ,Immunology ,Cancer research ,Female ,Fluorouracil ,business ,CD8 ,Follow-Up Studies - Abstract
A prospective randomized trial on 312 patients with locally advanced cervical carcinoma (FIGO stages IB-IV) was carried out. The 5-year survival in 90 patients treated with radiotherapy and antitumor polysaccharide sizofiran, an extract from the culture broth of Schizophyllum commune Fries, in combination was significantly (P = 0.045) better than that in 82 patients treated with radiotherapy alone. Treatment with sizofiran and 5-fluorouracil in combination improved (P = 0.003) the 5-year survival in 60 patients treated with radiotherapy. In 244 cervical carcinoma patients, the percentage of activated CD8+ (CD8+HLA-DR+) T cells in the CD8+ T-cell subsets in peripheral lymphocytes increased significantly as the disease progressed. A similar tendency was observed in the percentage of activated CD4+ (CD4+HLA-DR+) T cells in the CD4+ T-cell subsets. These immunologic parameters were significantly increased by radiotherapy, but not by surgery. Sizofiran accelerated a recovery in the activated CD8+ T cells in the CD8+ T-cell subsets compared with that of sizofiran nontreated patients after radiotherapy. Our data show that possible immune impairment in cervical carcinoma may be caused by disturbances in cell-mediated immunity, and that sizofiran is an effective immunotherapeutic agent for cervical carcinoma because it stimulates a rapid recovery of the immunologic parameters impaired by radiotherapy.
- Published
- 1995
284. Mucinous adenofibroma of the ovary: case report of the endocrinologic findings
- Author
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Hiroshi Mizutani, Hitoshi Okamura, Kohji Miyazaki, Toshimitsu Tohya, and Hidetaka Katabuchi
- Subjects
endocrine system ,medicine.medical_specialty ,medicine.drug_class ,Estrone ,Ovary ,chemistry.chemical_compound ,Ovarian tumor ,Ovarian Mucinous Adenofibroma ,medicine ,Humans ,Testosterone ,Mucinous Adenofibroma ,Aged ,Gynecology ,Ovarian Neoplasms ,Estradiol ,business.industry ,Hyperthecosis ,Androstenedione ,Obstetrics and Gynecology ,Luteinizing Hormone ,medicine.disease ,medicine.anatomical_structure ,Oncology ,chemistry ,Female ,Gonadotropin ,Follicle Stimulating Hormone ,Luteinizing hormone ,business ,Adenofibroma - Abstract
Endocrine and clinicopathologic findings in a 77-year-old woman with ovarian mucinous adenofibroma of borderline malignancy are reported. The preoperative levels of testosterone, androstenedione, estrone, and estradiol in her peripheral blood were 91 ng/ml, 3.78 ng/ml, 82 pg/ml, and 35 pg/ml, respectively, abnormally high. Those of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) were < 0.5 mIU/ml and 12.1 mIU/ml, respectively. Total abdominal hysterectomy and bilateral salpingo-oophorectomy were performed. Pathologic diagnosis of the right ovarian tumor was mucinous adenofibroma of borderline malignancy with hyperthecosis. Postoperatively, the levels of sex steroids and gonadotropin returned to the normal range of a postmenopausal woman. The patient remains well 2 years and 6 months after the operation.
- Published
- 1994
285. Adenoma malignum: MR appearances mimicking nabothian cysts
- Author
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Hitoshi Okamura, Y. Fukumatsu, Hidetaka Katabuchi, Mutsumasa Takahashi, Kohji Miyazaki, and Yasuyuki Yamashita
- Subjects
Adult ,medicine.medical_specialty ,Pathology ,medicine.diagnostic_test ,business.industry ,Cysts ,Uterine Cervical Neoplasms ,Magnetic resonance imaging ,General Medicine ,Adenocarcinoma ,Middle Aged ,Magnetic Resonance Imaging ,Diagnosis, Differential ,Uterine Cervical Diseases ,Uterine cervix ,Adenoma malignum ,Medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Female ,Radiology ,business - Published
- 1994
286. Isolation, growth and characteristics of human ovarian surface epithelium
- Author
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Takashi Ohba, Y. Fukumatsu, Hitoshi Okamura, Miwa Nakamura, and Hidetaka Katabuchi
- Subjects
Adult ,Cytoplasm ,endocrine system ,Pathology ,medicine.medical_specialty ,Time Factors ,Population ,Cell Count ,Ovary ,Cell Separation ,Biology ,Epithelium ,Pathology and Forensic Medicine ,Tissue culture ,medicine ,Humans ,Doubling time ,Collagenases ,education ,Molecular Biology ,Cells, Cultured ,Cell Nucleus ,education.field_of_study ,Microvilli ,Histocytochemistry ,Cell Biology ,General Medicine ,Middle Aged ,Immunohistochemistry ,Molecular biology ,Microscopy, Electron ,Intercellular Junctions ,medicine.anatomical_structure ,Microscopy, Electron, Scanning ,Cytochemistry ,Ovarian Endometriosis ,Female ,Cell Division ,Fetal bovine serum - Abstract
The ovarian surface epithelium (OSE) is a key tissue in the pathogenesis of ovarian surface epithelial-stromal tumours and ovarian endometriosis, commonly encountered gynaecological diseases. Despite the high incidence of these diseases, experimental in vitro studies of OSE are few and so we used the scraping method with an enzymatic procedure to isolate human OSE and studied its characteristics in vitro. Nineteen normal ovaries were used. After incubation of the ovary for 40 min in collagenase type 1 solution (300 U/ml), the surface cells were removed by gentle scraping with a surgical blade. Cells obtained as a cluster after unit gravity sedimentation with 5% bovine serum albumin in medium 199 were cultured in medium 199 containing 15% fetal bovine serum. The viable cell number in a single ovary was 0.1-2.7 x 10(6). The outgrowth of cells started from a homogeneous population of single cells, and the cell population doubling time was between 7 and 10 days. Confluent monolayers were formed after 13-20 days and subcultured from one to three times. The monolayers mostly had a cobblestone appearance, and fusiform or polygonal cells were also observed. By cytochemistry, immunocytochemistry and scanning and transmission electron microscopy, the cells were shown to have characteristics of mesothelial OSE cells in short-term culture. This experimental approach was efficient in providing cultured human OSE, which can be utilized to investigate pathobiology and carcinogenesis.
- Published
- 1994
287. High-dose oral tegafur-uracil maintenance therapy in patients with uterine cervical cancer.
- Author
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Isao Sakaguchi, Takeshi Motohara, Fumitaka Saito, Kiyomi Takaishi, Yukitoshi Fukumatsu, Toshimitsu Tohya, Saburo Shibata, Hiroyuki Mimori, Hironori Tashiro, and Hidetaka Katabuchi
- Subjects
URACIL ,PYRIMIDINES ,CERVICAL cancer ,CONTROL groups ,PROGNOSIS ,RADIOTHERAPY - Abstract
Objective: The aim of this study was to determine the efficacy and toxicity of oral administration of tegafur-uracil (UFT) at a high dose, 600 mg/day, based on the tegafur dose, against uterine cervical cancer. Methods: This study consisted of a retrospective analysis. From April 1986 to March 1997, 309 patients with uterine cervical cancer were registered. Oral UFT was administered to 162 patients for maintenance therapy after an initial treatment (the UFT group). The other 147 patients were not treated with UFT (the control group). The survival rate was calculated for both groups and statistically analyzed using the log-rank test. Adverse events were compared between the UFT and control groups. Results: In the UFT group, 103 patients (63.6%) received UFT for ⩾90 days. The drug dose was 600 mg/day for 137 patients (84.6%) and 300 to 400 mg/day for the remainder. The overall survival rate was significantly higher in the UFT group than in the control group (p<0.05). The prognosis was particularly favorable in stage III cases, in cases of squamous cell carcinoma, and in cases that were treated by radiotherapy. The most frequent side effects were nausea/vomiting (12.2%), appetite loss (10.1%), and leukopenia/neutropenia (5.8%). Conclusion: High-dose oral UFT maintenance treatment prolonged the disease-free survival and overall survival of patients with uterine cervical cancer, particularly of those with advanced disease. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
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288. Oral Health Status of Pregnant Women in Kumamoto Prefecture --Kumamoto RAINBOW Project--.
- Author
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Sakura CHIGA, Takashi OHBA, Junya MIYOSHI, Daisuke TANOUE, Hiromi KAWASE, Takahiko KATOH, and Hidetaka KATABUCHI
- Published
- 2015
- Full Text
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289. 熊本県の妊婦における歯科健診の実態
- Author
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Sakura CHIGA, Takashi OHBA, Junya MIYOSHI, Daisuke TANOUE, Hiromi KAWASE, Takahiko KATOH, and Hidetaka KATABUCHI
- Published
- 2015
- Full Text
- View/download PDF
290. Clinical outcome of vaginal danazol suppository use in women with pelvic endometriosis
- Author
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Hidetaka Katabuchi, J. Suzuki, R. Honda, Yoshinori Okamura, Takashi Ohba, and Hitoshi Okamura
- Subjects
Gynecology ,Danazol ,medicine.medical_specialty ,Pelvic endometriosis ,Reproductive Medicine ,business.industry ,medicine ,Obstetrics and Gynecology ,Suppository ,business ,medicine.drug - Published
- 2008
291. Ultrastructure of smooth muscle tissue in the female reproductive tract: uterus and oviduct
- Author
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Hitoshi Okamura, Shingo Fujii, Ikuo Konishi, and Hidetaka Katabuchi
- Subjects
medicine.anatomical_structure ,Smooth muscle tissue ,urogenital system ,Chemistry ,medicine ,Ultrastructure ,Myometrium ,Uterus ,Oviduct ,Connective tissue ,Anatomy ,Cervix ,Corpus Uteri - Abstract
The uterus is a hollow organ consisting of an upper, primarily muscular part, the corpus uteri, and a lower part, predominantly connective tissue, the cervix. The oviducts open into the upper and lateral angles of the uterus, constituting a conduit between the endometrial and peritoneal cavities. The body of the uterus is composed of three layers of smooth muscle cells (myometrium): outer and inner longitudinal layers and a circular intermediate layer accompanied by a rich vasculature between the layers (1). The oviduct is also composed of three layers of smooth muscle cells (myosalpinx), as well as the myometrium, but on the whole, the muscle elements are thinner than those of the uterus.
- Published
- 1990
292. Preformed Wolffian duct regulates Müllerian duct elongation independently of canonical Wnt signaling or Lhx1 expression.
- Author
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MASAHIKO CHIGA, TOMOKO OHMORI, TAKASHI OHBA, HIDETAKA KATABUCHI, and RYUICHI NISHINAKAMURA
- Subjects
WOLFFIAN body ,WNT proteins ,GENE expression ,CELLULAR signal transduction ,FEMALE reproductive organs ,CHICKEN embryos - Abstract
The Müllerian duct gives rise to female reproductive organs, such as the oviduct and uterus. During gestation, the Wolffian duct, which generates male reproductive organs and the kidney, is formed, and the Müllerian duct then elongates caudally along the preformed Wolffian duct. Anatomical separation of these two ducts in chick embryos demonstrated that the Wolffian duct is required for Müllerian duct formation. Likewise, a few reports supported this notion in mice, including studies on Wnt9b mutant mice and Wolffian duct-specific Lhxl deletion. However, anatomical ablation of the Wolffian duct has not been established in mice. In this study, we addressed the importance of the interaction between these two reproductive ducts, by generating mice that specifically expressed a diphtheria toxin subunit in the Wolffian duct. While this genetic ablation of the Wolffian duct resulted in kidney hypoplasia/agenesis in both male and female mutant mice, the female mutant mice lacked the uterus, which is derived from the Müllerian duct. At mid-gestation, the Müllerian duct was truncated at the level where the mutant Wolffian duct was prematurely terminated, meaning that Müllerian duct elongation was dependent on the preformed Wolffian duct. However, Wnt9b expression in the Wolffian duct and the resultant canonical Wnt activity, as well as Lhx1 expression, were not affected in the mutant mice. These results suggest that the Wolffian duct regulates Müllerian duct elongation by currently unidentified mechanisms that are independent of canonical Wnt signaling or Lhx1 expression. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
293. Corosolic acid enhances the antitumor effects of chemotherapy on epithelial ovarian cancer by inhibiting signal transducer and activator of transcription 3 signaling.
- Author
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YUKIO FUJIWARA, KIYOMI TAKAISHI, JUNKO NAKAO, TSUYOSHI IKEDA, HIDETAKA KATABUCHI, MOTOHIRO TAKEYA, and YOSHIHIRO KOMOHARA
- Subjects
OVARIAN cancer treatment ,OVARIAN cancer ,CANCER chemotherapy ,MACROPHAGES ,STAT proteins ,ADJUVANT treatment of cancer ,ANTINEOPLASTIC agents ,DRUG resistance in cancer cells - Abstract
Resistance to chemotherapy poses a serious problem for the treatment of advanced epithelial ovarian cancer patients. The mechanisms of chemoresistance are complex and studies have implicated signal transducer and activator of transcription 3 (STAT3) signaling in the chemoresistance of cancer cells. The present study investigated whether corosolic acid (CA), which has been previously reported to be a STAT3 inhibitor, was able to increase the sensitivity to chemotherapeutic drugs in epithelial ovarian cancer cells. CA also markedly enhanced the anticancer effect of paclitaxel, cisplatin and doxorubicin. In addition, CA abrogated the cell-cell interactions between macrophages and epithelial ovarian cancer cells and inhibited the macrophage-induced activation of epithelial ovarian cancer cells. These data indicated that CA was able to reverse the chemoresistance of epithelial ovarian cancer cells and suppress the cell-cell interaction with tumorigenic macrophages. Thus, CA may be useful as an adjuvant treatment to patients with advanced ovarian and other types of cancer due to the multiple anticancer effects. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
294. Distribution and cytological properties of macrophages in human fallopian tubes
- Author
-
Yoshito Suenaga, Hidetaka Katabuchi, and Hitoshi Okamura
- Subjects
Pathology ,medicine.medical_specialty ,business.industry ,Obstetrics and Gynecology ,Medicine ,Distribution (pharmacology) ,General Medicine ,business - Published
- 2000
295. Macrophages are involved in secretion of HCG and its degradation
- Author
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Hidetaka Katabuchi, Salma Khan, Hitoshi Okamura, and R. Nishimura
- Subjects
Reproductive Medicine ,Chemistry ,Obstetrics and Gynecology ,Degradation (geology) ,Secretion ,Developmental Biology ,Cell biology - Published
- 1998
296. Molecular forms of human chorionic gonadotropin in body fluids in gestational trophoblastic disease
- Author
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Hidetaka Katabuchi, Masako Araki, Salma Khan, Hitoshi Okamura, and R. Nishimura
- Subjects
medicine.medical_specialty ,Endocrinology ,Reproductive Medicine ,business.industry ,Gestational trophoblastic disease ,Internal medicine ,Obstetrics and Gynecology ,Medicine ,business ,medicine.disease ,Developmental Biology ,Human chorionic gonadotropin - Published
- 1997
297. Classifications of ovarian cancer tissues by proteomic patterns.
- Author
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Yi Zhu, Rong Wu, Navneet Sangha, Chul Yoo, Kathleen R. Cho, Kerby A. Shedden, Hidetaka Katabuchi, and David M. Lubman
- Published
- 2006
- Full Text
- View/download PDF
298. Distribution and regulation of Ca2+/calmodulin-dependent protein kinase II in the rat ovary
- Author
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Takashi Ohbai, Kohji Fukunaga, Eishichi Miyamoto, Kohji Miyazaki, Hidetaka Katabuchi, and Hitoshi Okamura
- Subjects
Pharmacology ,medicine.anatomical_structure ,Chemistry ,medicine ,Distribution (pharmacology) ,Ovary ,Ca2 calmodulin ,Protein kinase II ,Cell biology - Published
- 1991
299. A case of eclampsia during the 18th week of gestation
- Author
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Kazuo Matsui, Masaharu Ito, Mahito Nakayama, Masao Maeyama, Toshihiro Yoshimura, and Hidetaka Katabuchi
- Subjects
Adult ,medicine.medical_specialty ,Time Factors ,Biopsy ,Pregnancy Complications, Cardiovascular ,Disease ,Kidney ,Pregnancy ,Seizures ,medicine ,Humans ,Eclampsia ,Rh Isoimmunization ,Fetal Death ,reproductive and urinary physiology ,Gynecology ,medicine.diagnostic_test ,business.industry ,Obstetrics ,Obstetrics and Gynecology ,Gestational age ,General Medicine ,medicine.disease ,female genital diseases and pregnancy complications ,embryonic structures ,Gestation ,Female ,Renal biopsy ,business - Abstract
We report a rare case of eclampsia which occurred during the 18th gestational week in the absence of hydatidiform disease or Rh isoimmunization. In spite of extensive examinations during pregnancy and postpartum, we were unable to find any underlying disease, and the patient returned to being normotensive and non-proteinuric postpartum.
- Published
- 1985
300. Cytopathological study on macrophages of placental villi. 1. Mainly on cytological properties of Hofbauer cells
- Author
-
Hidetaka Katabuchi
- Subjects
Biology - Published
- 1986
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