Author: "Gröbe, A" - Searchworks@Jio Institute Digital Library Search Results

1,062 results on '"Gröbe, A"'

251. Granulozytentransfusion zur Behandlung septischer Zustände bei neutropenischen Patienten

Author: Kamanabroo, D., Jansen, M., Gröbe, H., Büchner, Th., and Schlegel, B., editor
Published: 1978
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252. Amino Acid Analysis of Hair in Inherited Diseases

Author: Gröbe, H., Happle, Rudolf, editor, and Grosshans, Edouard, editor
Published: 1987
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253. A hematogenous spread brain abscess invading the right damaged temporomandibular joint

Author: Manfred Westphal, Jan Klatt, Max Heiland, Alexander Gröbe, Philipp Pohlenz, and Rainer Schmelzle
Subjects: Cartilage, Articular, musculoskeletal diseases, Microsurgery, medicine.medical_specialty, Fossa, Brain Abscess, Osteolysis, Osteoarthritis, Neurosurgical Procedures, stomatognathic system, Facial Pain, Temporomandibular Joint Disc, medicine, Humans, Abscess, Brain abscess, medicine.diagnostic_test, biology, business.industry, Cartilage, Headache, Temporal Bone, Magnetic resonance imaging, Middle Aged, Temporomandibular Joint Disorders, Hematogenous Spread, medicine.disease, biology.organism_classification, Surgery, Temporomandibular joint, stomatognathic diseases, medicine.anatomical_structure, Otorhinolaryngology, Drainage, Female, Oral Surgery, business, Follow-Up Studies
Abstract: We report a rare case of a brain abscess which drained spontaneously in a temporomandibular joint damaged by osteoarthritis. The female patient presented to our hospital with a severe headache and pain in the temporomandibular joint. She showed elevated inflammatory parameters with unknown cause. Magnetic resonance imaging of her whole body revealed a large temporal brain abscess extending into the glenoid fossa of the temporomandibular joint. The brain abscess was incised and drained by neurosurgeons in our hospital and in the same operation we resected the articular disc and the affected part of the right temporomandibular joint. Histological examination confirmed a chronic arthrosis in the resected bone of the temporomandibular joint and an inflammatory abscess in the resected brain tissue. The patient recovered well and the inflammation resolved as seen in postoperative investigations. Magnetic resonance imaging a month later confirmed local consolidation in the brain with no sign of residual inflammation.
Published: 2012
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254. Assistierte Reproduktion

Author: A. Feige and H. Gröbe
Subjects: Gynecology, medicine.medical_specialty, Assistierte reproduktion, media_common.quotation_subject, medicine, Obstetrics and Gynecology, Sociology, Reproduction, media_common
Published: 2002
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255. Evaluation of user impressions, micromorphological bone surface properties and material attrition by three generations of osteotomy methods

Author: Max Heiland, Ashkan Rashad, Henning Hanken, Alexander Gröbe, Ralf Smeets, Benedicta Beck-Broichsitter, and Carsten Rendenbach
Subjects: Otorhinolaryngology, business.industry, medicine.medical_treatment, medicine, Dentistry, Surgery, Attrition, Oral Surgery, Three generations, medicine.disease, Osteotomy, business, Bone surface
Published: 2017
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256. The outcome of patients suffering from oral squamous cell carcinoma treated under certified tumour board conditions

Author: A. Gröbe
Subjects: Oncology, medicine.medical_specialty, Otorhinolaryngology, business.industry, Internal medicine, medicine, Surgery, Basal cell, Oral Surgery, business, Outcome (game theory)
Published: 2017
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257. Linear Accuracy and Reliability of Volume Data Sets Acquired by Two CBCT-Devices and a MSCT using Virtual Models - A Comparative in-vitro Study

Author: Wikner, Johannes, Hanken, Henning, Heiland, Max, Gröbe, Alexander, and Friedrich, Reinhard
Subjects: ddc: 610, 610 Medical sciences, Medicine
Abstract: Aim: To discriminate clinically relevant aberrance, accuracy of linear measurements in three-dimensional reconstructed data-sets was investigated. Methods: Three partly edentulous human skulls were examined. Landmarks were defined prior to acquisition. Two CBCT-Scanners and a Quad-slice CT-Scanner[for full text, please go to the a.m. URL], 52. Jahrestagung der Deutschen Gesellschaft für Plastische und Wiederherstellungschirurgie (DGPW)
Published: 2014

258. Local full-thickness skin graft of the donor arm--a novel technique for the reduction of donor site morbidity in radial forearm free flap

Author: Johannes Wikner, Alexander Gröbe, M Blessmann, Alexandre T Assaf, Max Heiland, Björn Riecke, and A. Al-Dam
Subjects: Novel technique, Adult, Male, medicine.medical_specialty, medicine.medical_treatment, Free Tissue Flaps, Defect closure, Forearm, Medicine, Humans, Prospective Studies, Reduction (orthopedic surgery), Aged, Aged, 80 and over, Wound Healing, integumentary system, Skin incision, business.industry, Suture Techniques, Local flap, Full-thickness skin graft, Skin Transplantation, Middle Aged, Plastic Surgery Procedures, Surgery, medicine.anatomical_structure, Otorhinolaryngology, Radial forearm free flap, Head and Neck Neoplasms, Radial Artery, Female, Oral Surgery, business
Abstract: A novel technique to reduce donor site morbidity after radial forearm free flap (RFFF) harvest, using a local full-thickness skin graft (FTSG), is described. Thirty consecutive patients undergoing RFFF for head and neck reconstruction were enrolled in a prospective study. Donor site defect closure was performed with spindle-shaped FTSGs excised from the wavelike skin incision made for the vascular pedicle. Both the removal site of the FTSG on the volar forearm and the covered RFFF donor site healed uneventfully in 29 cases, with no impairment of function related to the skin graft. No skin graft failure and no exposure, tenting, or adherence of the flexor tendons occurred. All patients expressed satisfaction with postoperative pain, the functional outcome, and cosmetic appearance. Primary donor site defect closure could be achieved in all cases with the use of a local FTSG. This graft can be gained at the access incision for the vascular pedicle, avoids expansion of the incision for a local flap technique, and does not prolong wound healing, and thus reduces both donor site and graft site morbidity of the RFFF. This technique leads to an inconspicuous aesthetic result with no apparent relevant functional deficits and avoids the need for a second donor site.
Published: 2014

259. Bone substitutes enhance osteogenic differentiation of mesenchymal stem cells in three-dimensional scaffolds

Author: Henning, Hanken, Janna, Alpers, Melanie, Böbel, Michael, Wöltje, Philip, Hartjen, Reinhard E, Friedrich, Ole, Jung, Carsten, Rendenbach, Alexander, Gröbe, Max, Heiland, Ahmad, Al-Dam, Wolfgang, Eichhorn, and Ralf, Smeets
Subjects: Tissue Scaffolds, Cell Survival, Osteogenesis, Bone Substitutes, Cell Culture Techniques, Animals, Cell Differentiation, Mesenchymal Stem Cells, In Vitro Techniques, Alkaline Phosphatase, Cells, Cultured, Rats
Abstract: The present study aimed to find bone substitutes to enhance osteogenic differentiation of mesenchymal stem cells in three-dimensional scaffolds in the absence of dexamethasone.Seven commercial bone substitutes were added to a three-dimensional fibrin-matrix containing rat mesenchymal stem cells in a biocompatible poly-L-lactic-acid mesh. Cell viability, cytotoxicity and alkaline phosphatase activity were followed for three weeks. Expression of bone markers was examined by qualitative evaluation of corresponding transcripts.Six out of the seven bone derivatives exhibited an osteogenic-enhancing effect.The osteogenic-enhancing effect of the evaluated bone substitutes suggests their potential clinical application for preparation of autologous bone replacement material in three-dimensional carriers.
Published: 2014

260. Her2 expression and gene amplification is rarely detectable in patients with oral squamous cell carcinomas

Author: Max Heiland, Alexander Gröbe, J. R. Izbicki, Susanne Sehner, Wolfgang Eichhorn, Meike Fraederich, Ralf Smeets, Katharina Grupp, Ronald Simon, Marco Blessmann, Guido Sauter, Robert Gaudin, and Henning Hanken
Subjects: Adult, Male, Cancer Research, Pathology, medicine.medical_specialty, Receptor, ErbB-2, Cell, Pathology and Forensic Medicine, Breast cancer, Gene duplication, medicine, Humans, skin and connective tissue diseases, Mouth Floor, In Situ Hybridization, Fluorescence, Aged, Neoplasm Staging, Aged, 80 and over, Tissue microarray, medicine.diagnostic_test, business.industry, Gene Amplification, Pharyngeal Neoplasms, Middle Aged, medicine.disease, Head and neck squamous-cell carcinoma, Immunohistochemistry, Squamous carcinoma, Gene Expression Regulation, Neoplastic, Survival Rate, stomatognathic diseases, medicine.anatomical_structure, Phenotype, Otorhinolaryngology, Head and Neck Neoplasms, Tissue Array Analysis, Carcinoma, Squamous Cell, Periodontics, Female, Mouth Neoplasms, Lymph Nodes, Oral Surgery, Neoplasm Grading, Neoplasm Recurrence, Local, business, Fluorescence in situ hybridization, Follow-Up Studies
Abstract: Purpose Her2 (ErbB2) transforms cells when overexpressed and is an important therapeutic target in breast cancer. Contrary to breast cancer, studies on Her2 overexpression and gene amplification in squamous cell carcinomas of the head and neck region described largely different results. This study was undertaken to learn more on the prevalence and clinical significance of HER2 amplification and overexpression in squamous cell carcinomas of the head and neck. Materials and methods Her2 expression and gene amplification was analyzed by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) on two tissue microarrays composed of 427 squamous cell carcinomas of the head and neck region and 222 oral squamous cell carcinomas. Results were compared with clinicopathological features. Results Her2 expression and gene amplification was rarely detectable in squamous cell carcinomas of the head and neck region and unrelated to tumor phenotype or survival of the patients with oral squamous carcinoma. Discussion Our results demonstrate that Her2 protein and gene amplification was only detectable in a small subset of squamous cell carcinomas of the head and neck region as well as oral squamous cell carcinomas. However, it can be speculated that those few patients with Her2 overexpressing and gene amplificated tumors may possibly benefit from an anti-Her2 therapy.
Published: 2014

261. Virtual planning of complex head and neck reconstruction results in satisfactory match between real outcomes and virtual models

Author: Björn Riecke, Oliver Vorwig, Alexander Gröbe, Clemens Schablowsky, Ahmed Al-Dam, Henning Hanken, Susanne Sehner, Max Heiland, Ibrahim Nourwali, and Ralf Smeets
Subjects: Adult, Male, medicine.medical_specialty, Surgical Flaps, Resection, Ilium, User-Computer Interface, Form and function, medicine, Postoperative results, Humans, Mandibular Diseases, Defect size, Head and neck, General Dentistry, Aged, Retrospective Studies, Bone Transplantation, business.industry, Angiography, Retrospective cohort study, Middle Aged, Plastic Surgery Procedures, Surgery, Oral, Confidence interval, Maxillary Diseases, Surgery, Treatment Outcome, Virtual planning, Fibula, Computer-Aided Design, Radiographic Image Interpretation, Computer-Assisted, Female, business, Tomography, X-Ray Computed
Abstract: The reconstruction of large facial bony defects using microvascular transplants requires extensive surgery to achieve full rehabilitation of form and function. The purpose of this study is to measure the agreement between virtual plans and the actual results of maxillofacial reconstruction. This retrospective cohort study included 30 subjects receiving maxillofacial reconstruction with a preoperative virtual planning. Parameters including defect size, position, angle and volume of the transplanted segments were compared between the virtual plan and the real outcome using paired t test. A total of 63 bone segments were transplanted. The mean differences between the virtual planning and the postoperative situation were for the defect sizes 1.17 mm (95 % confidence interval (CI) (−.21 to 2.56 mm); p = 0.094), for the resection planes 1.69 mm (95 % CI (1.26–2.11); p = 0.033) and 10.16° (95 % CI (8.36°–11.96°); p
Published: 2014

262. Lack of evidence for predictive and prognostic value of cyclin D1 gene polymorphism CCND1 G870A for oral squamous cell carcinoma

Author: Artun, Atac, Björn, Riecke, Alexandre T, Assaf, Alexander, Gröbe, Reinhard E, Friedrich, Max, Heiland, Jakob R, Izbicki, Lan, Kluwe, and Marco, Blessmann
Subjects: Male, Middle Aged, Prognosis, Polymorphism, Single Nucleotide, Survival Rate, Case-Control Studies, Lymphatic Metastasis, Carcinoma, Squamous Cell, Humans, Cyclin D1, Female, Mouth Neoplasms, Neoplasm Recurrence, Local, Follow-Up Studies, Neoplasm Staging
Abstract: Cyclin D1 gene (CCND1) has a G to A polymorphism at the splice donor site of exon 4, position 870. The A allele codes for a truncated variant, cyclin D1b, which may have higher transforming activity. Data regarding the predictive and prognostic value of the CCND1 G870A polymorphism in tumors are controversial. We aimed to examine this polymorphism in patients with oral carcinoma.Genotyping of CCND1 G870A was determined by means of direct sequencing in 83 patients with oral carcinomas and in 102 healthy controls. Association with clinical outcomes was evaluated statistically.We failed to find any significant association of CCND1 G870A with risk of oral carcinomas in this German population, with clinical and pathological features of the tumours or with overall survival of the patients.Our results suggest that CCND1 G870A has no, or only very limited, predictive and prognostic value for oral carcinoma.
Published: 2014

263. Die virtuelle Planung komplexer Rekonstruktionen im Kopf-Hals Bereich – wie präzise ist die virtuelle Planung umsetzbar?

Author: Hanken, H, Heiland, M, Gröbe, A, Blessmann, M, Riecke, B, Al-Dam, A, Vorwig, O, and Smeets, R
Subjects: ddc: 610, 610 Medical sciences, Medicine
Abstract: Einleitung: Die Rekonstruktion von ausgedehnten knöchernen Defekten im Kopf- Halsbereich mittels mikrovaskulärer Transplantate ist aufwendig und langwierig um eine optimale dreidimensionale Situation wiederherzustellen. In den letzten Jahren wurde zur Unterstützung des Chirurgen die virtuelle[for full text, please go to the a.m. URL], 131. Kongress der Deutschen Gesellschaft für Chirurgie
Published: 2014
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264. Face Transplantation: On the Verge of Becoming Clinical Routine?

Author: Smeets, Ralf, Rendenbach, Carsten, Birkelbach, Moritz, Al-Dam, Ahmed, Gröbe, Alexander, Hanken, Henning, and Heiland, Max
Subjects: Article Subject
Abstract: Introduction. Face transplantation (FT) is an innovative achievement of modern reconstructive surgery and is on the verge of becoming a common surgical opportunity. This review article was compiled to provide an update on this surgical field, especially regarding clinical outcomes, benefits, and complications implied. Methods. We performed an extensive research on all English-language Medline articles, case reports, and reviews published online until September 15, 2013. Used search terms were “face transplantation,” “face transplant,” “facial transplantation,” “facial transplant,” “face allograft,” and “facial allograft.” Results. To date 27 FTs have been performed worldwide. 19 of these cases have been published in the Medline database. Long-term follow-up reports of FT cases are rare. Three deaths associated with the procedure have occurred to date. The clinical outcomes of FT are satisfying. Reinnervation of sensation has been faster than motor recovery. Extensive functional improvements have been observed. Due to strict immunosuppression protocols, no case of hyperacute or chronic rejection and no graft-versus-host disease have occurred to date. Conclusions. As studies on long-term outcomes are missing, particularly regarding immunosuppression-related complications, FT will stay experimental for the next years. Nevertheless, for a small group of patients, FT already is a feasible reconstructive option.
Published: 2014
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265. Die Entwicklung von individuell herstellbaren, bioresorbierbaren Magnesiumimplantaten für Knochendefekte – eine In-vitro-Analyse

Author: Hanken, H, Jung, O, Heiland, M, Gröbe, A, Ptock, C, Schwade, M, Kopp, A, Hartjen, P, and Smeets, R
Subjects: ddc: 610, 610 Medical sciences, Medicine
Abstract: Einleitung: Großvolumige Knochendefekte nach Trauma- oder Tumorresektionen im Gesichtsbereich erfordern den Einsatz von Knochentransplantaten oder rekonstruktiven Materialien wie Titan. Herkömmliche Verfahren ziehen oft erneute operative Eingriffe mit entsprechenden Morbiditäten und möglichen[for full text, please go to the a.m. URL], 52. Jahrestagung der Deutschen Gesellschaft für Plastische und Wiederherstellungschirurgie (DGPW)
Published: 2014
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266. Die klinische Versorgung von sehr unreifen Frühgeborenen in Deutschland, Österreich und der Schweiz

Author: Gröbe, Sascha Claus
Subjects: national survey, delivery room management, resuscitation, VLBW
Abstract: Hintergrund: Studien aus den USA, Australien und Spanien zeigten signifikante inter-institutionelle Unterschiede hinsichtlich der Versorgung von Frühgeborenen mit einem Geburtsgewicht von unter 1500g (VLBWI, Very Low Birth Weight Infant). Diese Unterschiede fanden sich trotz anerkannter, evidenz- basierter internationaler Richtlinien (ILCOR). Fragestellung: Existieren Unterschiede in der apparativen Ausstattung und im Vorgehen bei der Versorgung von VLBW-Kindern in Deutschland, Österreich und der Schweiz? Bestehen Unterschiede zwischen universitären und nicht-universitären Einrichtungen und inwieweit ist dieses Vorgehens mit internationalen Richtlinien konform? Methodik: Prospektive Fragebogenerhebung von 249 neonatologischen Einrichtungen in Deutschland, Österreich und der Schweiz, statistische Analyse und Vergleich mit entsprechenden internationalen Studien nach systematischer, internet-basierter Literaturrecherche. Ergebnisse: Rücklaufquote 76%, 25% universitäre und 75% nicht-universitäre Abteilungen. Signifikante Unterschiede: weniger Schweizer Abteilungen haben einen über 24 Stunden verfügbaren neonatologischen Versorgungsdienst, seltener einen definierten Erstversorgungsraum und einen geringeren Anteil an Geräten, die eine Druckkontrolle erlauben. Deutsche Abteilungen führen signifikant häufiger ein Blähmanöver durch. Keine Unterschiede fanden sich bei der Wahl der initial verwendeten Sauerstoffkonzentration, bei den Zielwerten der Sauerstoffsättigung oder bei invasiven Prozeduren. 31% der Abteilungen beginnen eine Atemunterstützung mit Raumluft, 72% wählen eine Zielsauerstoffsättigung von 85-90% (10. Lebensminute) und rund 40% verwenden druckkontrollierte Atemhilfen. Universitäre und nicht-universitäre Abteilungen unterscheiden sich nur geringfügig: Nicht-universitäre Abteilungen verwenden seltener selbst entfaltende Beatmungsbeutel, wählen häufiger eine höhere Zielsauerstoffsättigung und verwenden mehr Kopfbedeckungen zum Wärmeschutz als universitäre Abteilungen. Universitäre Abteilungen wählen höhere Surfactant- Dosen. Die Empfehlungen der ILCOR-Richtlinien (2005) werden in allen untersuchten Ländern und Abteilungsformen weitestgehend umgesetzt, nur die empfohlenen CO2-Detektoren zur Kontrolle der Tubuslage werden kaum verwendet. Schlussfolgerung: Die Versorgung von VLBWI ist in den untersuchten Ländern, bis auf wenige Unterschiede bei der apparativen Ausstattung und einzelner räumlicher und organisatorischer Merkmale, als gleichwertig anzusehen. Weder die apparative Ausstattung noch die klinische Vorgehensweise an akademischen oder nicht-akademischen Einrichtungen unterscheidet sich wesentlich, obwohl die nicht-akademischen Einrichtungen signifikant weniger Geburten und signifikant weniger VLBWI pro Jahr betreuen. Die befragten Einrichtungen boten im Jahr 2008 eine den ILCOR-Richtlinien entsprechende Versorgung, in der sich bereits neuste wissenschaftliche Erkenntnisse hinsichtlich einer modernen nicht invasiven Atemunterstützung umgesetzt zeigten. Im Vergleich zu anderen Ländern sind diese Maßnahmen wie auch die dafür notwenige Ausstattung zu einem höheren Grad und in allen Einrichtungen gleichermaßen etabliert. Fazit: Die optimale Versorgung von Frühgeborenen basiert nicht nur auf der Beachtung von Richtlinien und einer entsprechenden apparativen Ausstattung, sondern erfordert auch die Umsetzung von Erkenntnissen aus aktuellen klinischen Studien., Backround: The international liaison committee for resuscitation (ILCOR) provides regularly updated guidelines on resuscitation for preterm infants. Despite these guidelines, surveys from the USA, Australia and Spain have shown significant inter-institutional variation of delivery room (DR) management for very low birth weight infants (VLBWI, < 1500g). Objektive: To investigate protocols for DR management of VLBWI in Germany, Austria and Switzerland, and to compare these with the 2005 ILCOR guidelines and international surveys. Methods: DR management, parameter settings, target parameters and equipment used were surveyed in a prospective, questionnaire-based survey in 2008. Findings were compared between countries, between academic and non-academic units and checked for compliance with the ILCOR 2005 guidelines. Results: 190 of 249 departments participated in the questionnaire survey, the response rate was 76%. Of these, 25 % are university and 75% are non-university departments. A 24 hours in house neonatal service is less frequently provided in Switzerland and a designated resuscitation area is significant less available in this country. Swiss units have a lower proportion of devices which allow pressure control. Sustained inflations were performed more often in German units. No differences were found regarding application and monitoring of supplementary oxygen, targeted saturation levels and invasive procedures such as the application of Surfactant via INSURE. 31% of all units start resuscitation with air, 72% have a targeted level of oxygen saturation of 85-90%. 40% use equipment which allows pressure control. Comparison of academic and non-academic units shows only minor differences: Non-academic units chose more often higher targeted oxygen saturation levels, use less Self inflating bags and tend do use more head covers for thermal management. Academic units chose higher Surfactant doses. CO2-detectors for confirmation of correct tube placement, as advised by ILCOR, were used in only 10% of units. Otherwise we found good accordance with the ILCOR guidelines. Summary: Neither the equipment or facilities nor the clinical approach towards resuscitation differed between the compared countries, and academic and non- academic institutions. Although, the non-academic institutions serve significantly fewer VLBWI. DR management is in good accordance with the 2005 ILCOR guidelines and implements also the latest scientific findings. Compared to results from other international surveys, these measures and the required equipment are established equally and at a more complete level. Conclusion: The optimal care of preterm infants is not only based on the adherence to international guidelines, but also by implementing relevant findings from recent clinical research.
Published: 2014

267. Mutations of arginine residues within the 146-KKRRK-150 motif of the ActA protein of Listeria monocytogenes abolish intracellular motility by interfering with the recruitment of the Arp2/3 complex

Author: Jürgen Wehland, Laura M. Machesky, Eugen Domann, Trinad Chakraborty, Antonio Sechi, Susanne Pistor, Birgit Gerstel, and Lothar Gröbe
Subjects: Intracellular Fluid, Arginine, Amino Acid Motifs, Molecular Sequence Data, Mutant, Motility, Arp2/3 complex, macromolecular substances, Biology, medicine.disease_cause, Cell Line, Bacterial Proteins, Listeria monocytogenes, medicine, Animals, Point Mutation, Amino Acid Sequence, Actin, Sequence Deletion, Cell Membrane, Membrane Proteins, Biological Transport, Cell Biology, Chromosomes, Bacterial, Bridged Bicyclo Compounds, Heterocyclic, Molecular biology, Actins, Cell biology, Cytoskeletal Proteins, Thiazoles, Genes, Bacterial, Actin-Related Protein 3, Paracytophagy, Actin-Related Protein 2, Mutagenesis, Site-Directed, biology.protein, Thiazolidines, Intracellular
Abstract: The recruitment of actin to the surface of intracellular Listeria monocytogenes and subsequent tail formation is dependent on the expression of the bacterial surface protein ActA. Of the different functional domains of ActA identified thus far, the N-terminal region is absolutely required for actin filament recruitment and intracellular motility. Mutational analysis of this domain which abolished actin recruitment by intracellular Listeria monocytogenes identified two arginine residues within the 146-KKRRK-150 motif that are essential for its activity. More specifically, recruitment of the Arp2/3 complex to the bacterial surface, as assessed by immunofluorescence staining with antibodies raised against the p21-Arc protein, was not obtained in these mutants. Consistently, treatment of infected cells with latrunculin B, which abrogated actin filament formation, did not affect association of ActA with p21-Arc at the bacterial surface. Thus, the initial recruitment of the Arp2/3 complex to the bacterial surface is independent of, and precedes, actin polymerisation. Our data suggest that binding of the Arp2/3 complex is mediated by specific interactions dependent on arginine residues within the 146-KKRRK-150 motif present in ActA.
Published: 2000
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268. Role of Proteins of the Ena/VASP Family in Actin-based Motility of Listeria monocytogenes

Author: Frank B. Gertler, Lothar Gröbe, Brigitte M. Jockusch, Jürgen Wehland, Birgit Harbeck, Thomas P. Loisel, Marie-France Carlier, Ann M. Wehman, and Valérie M. Laurent
Subjects: Blood Platelets, Movement, Ena/VASP/Mena, Motility, macromolecular substances, Models, Biological, Focal adhesion, Mice, Profilins, 03 medical and health sciences, Contractile Proteins, 0302 clinical medicine, EVH1 domain, Animals, Cytoskeleton, Actin, DNA Primers, 030304 developmental biology, Mice, Knockout, platelet, 0303 health sciences, Binding Sites, Base Sequence, actin polymerization, biology, Microfilament Proteins, Brain, Proteins, Ena/Vasp homology proteins, Cell Biology, Phosphoproteins, Listeria monocytogenes, Actins, Cell biology, DNA-Binding Proteins, Cytoskeletal Proteins, Microscopy, Electron, motility, Profilin, Phosphoprotein, biology.protein, Carrier Proteins, Cell Adhesion Molecules, 030217 neurology & neurosurgery, Protein Binding, Regular Articles
Abstract: Intracellular propulsion of Listeria monocytogenes is the best understood form of motility dependent on actin polymerization. We have used in vitro motility assays of Listeria in platelet and brain extracts to elucidate the function of the focal adhesion proteins of the Ena (Drosophila Enabled)/VASP (vasodilator-stimulated phosphoprotein) family in actin-based motility. Immunodepletion of VASP from platelet extracts and of Evl (Ena/VASP-like protein) from brain extracts of Mena knockout (−/−) mice combined with add-back of recombinant (bacterial or eukaryotic) VASP and Evl show that VASP, Mena, and Evl play interchangeable roles and are required to transform actin polymerization into active movement and propulsive force. The EVH1 (Ena/VASP homology 1) domain of VASP is in slow association–dissociation equilibrium high-affinity binding to the zyxin-homologous, proline-rich region of ActA. VASP also interacts with F-actin via its COOH-terminal EVH2 domain. Hence VASP/ Ena/Evl link the bacterium to the actin tail, which is required for movement. The affinity of VASP for F-actin is controlled by phosphorylation of serine 157 by cAMP-dependent protein kinase. Phospho-VASP binds with high affinity (0.5 × 108 M−1); dephospho-VASP binds 40-fold less tightly. We propose a molecular ratchet model for insertional polymerization of actin, within which frequent attachment–detachment of VASP to F-actin allows its sliding along the growing filament.
Published: 1999
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269. Materialentfernung in der kraniomaxillofazialen Traumatologie

Author: Ebker, Tobias, additional, Lohse, Christian, additional, Hanken, Henning, additional, Heiland, Max, additional, and Gröbe, Alexander, additional
Published: 2016
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270. Novel silk protein barrier membranes for guided bone regeneration

Author: Smeets, Ralf, primary, Knabe, Christine, additional, Kolk, Andreas, additional, Rheinnecker, Michael, additional, Gröbe, Alexander, additional, Heiland, Max, additional, Zehbe, Rolf, additional, Sachse, Manuela, additional, Große‐Siestrup, Christian, additional, Wöltje, Michael, additional, and Hanken, Henning, additional
Published: 2016
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271. CD151 expression is frequent but unrelated to clinical outcome in head and neck cancer

Author: Nienstedt, Julie C., primary, Gröbe, Alexander, additional, Lebok, Patrick, additional, Büscheck, Franziska, additional, Clauditz, Till, additional, Simon, Ronald, additional, Heumann, Asmus, additional, Sauter, Guido, additional, Moebius, Christoph, additional, Münscher, Adrian, additional, Knecht, Rainald, additional, Blessmann, Marco, additional, Heiland, Max, additional, and Pflug, Christina, additional
Published: 2016
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272. Knochenersatzmaterialien

Author: Smeets, R., primary, Hanken, H., additional, Beck-Broichsitter, B., additional, Gröbe, A., additional, Precht, C., additional, Heiland, M., additional, and Jung, O., additional
Published: 2016
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273. Obstacles in spatial evaluation of CBCT-reformatted panoramic imaging

Author: Wikner, Johannes, primary, Friedrich, Reinhard E, additional, Rashad, Ashkan, additional, Schulze, Dirk, additional, Hanken, Henning, additional, Heiland, Max, additional, Gröbe, Alexander, additional, and Riecke, Björn, additional
Published: 2016
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274. Survival and overall treatment time after postoperative radio(chemo)therapy in patients with head and neck cancer

Author: Tribius, Silke, primary, Donner, Johanna, additional, Pazdyka, Henning, additional, Münscher, Adrian, additional, Gröbe, Alexander, additional, Petersen, Cordula, additional, Krüll, Andreas, additional, and Tennstedt, Pierre, additional
Published: 2016
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275. Ein neuer Weg in der rekonstruktiven Kopf-Halschirurgie: resorbierbare patientenspezifische Magnesium-Scaffolds

Author: Smeets, R, Gröbe, A, Kopp, A, Ptock, C, Eichhorn, W, Hartjen, P, Beck-Broichsitter, B, Riecke, B, Heiland, M, Hanken, H, Smeets, R, Gröbe, A, Kopp, A, Ptock, C, Eichhorn, W, Hartjen, P, Beck-Broichsitter, B, Riecke, B, Heiland, M, and Hanken, H
Published: 2015

276. Steroid hydroxylation by basidiomycete peroxygenases: A combined experimental and computational study, Applied and environmental microbiology

Author: Barcelona Supercomputing Center, Babot, Esteban D., del Río, José C., Cañellas, Marina, Sancho, Ferran, Lucas, Fatima, Guallar, Víctor, Kalum, Lisbeth, Lund, Henrik, Gröbe, Glenn, Scheibner, Katrin, Ullrich, René, Hofrichter, Martin, Martínez, Ángel T., Gutiérrez, Ana, Barcelona Supercomputing Center, Babot, Esteban D., del Río, José C., Cañellas, Marina, Sancho, Ferran, Lucas, Fatima, Guallar, Víctor, Kalum, Lisbeth, Lund, Henrik, Gröbe, Glenn, Scheibner, Katrin, Ullrich, René, Hofrichter, Martin, Martínez, Ángel T., and Gutiérrez, Ana
Abstract: The goal of this study is the selective oxyfunctionalization of steroids under mild and environmentally friendly conditions using fungal enzymes. With this purpose, peroxygenases from three basidiomycete species were tested for the hydroxylation of a variety of steroidal compounds, using H2O2 as the only cosubstrate. Two of them are wild-type enzymes from Agrocybe aegerita and Marasmius rotula, and the third one is a recombinant enzyme from Coprinopsis cinerea. The enzymatic reactions on free and esterified sterols, steroid hydrocarbons, and ketones were monitored by gas chromatography, and the products were identified by mass spectrometry. Hydroxylation at the side chain over the steroidal rings was preferred, with the 25-hydroxyderivatives predominating. Interestingly, antiviral and other biological activities of 25-hydroxycholesterol have been reported recently (M. Blanc et al., Immunity 38:106–118, 2013, http://dx.doi.org/10.1016/j.immuni.2012.11.004). However, hydroxylation in the ring moiety and terminal hydroxylation at the side chain also was observed in some steroids, the former favored by the absence of oxygenated groups at C-3 and by the presence of conjugated double bonds in the rings. To understand the yield and selectivity differences between the different steroids, a computational study was performed using Protein Energy Landscape Exploration (PELE) software for dynamic ligand diffusion. These simulations showed that the active-site geometry and hydrophobicity favors the entrance of the steroid side chain, while the entrance of the ring is energetically penalized. Also, a direct correlation between the conversion rate and the side chain entrance ratio could be established that explains the various reaction yields observed., This study was supported by the INDOX (KBBE-2013-7-613549), PEROXICATS (KBBE-2010-4-265397), and PELE (ERC-2009-Adg 25027) EU projects., Peer Reviewed, Postprint (published version)
Published: 2015

277. PO-0645: Does overall treatment time matter in patients with locally advanced head and neck cancer? An old chestnut revisited

Author: J. Donner, Silke Tribius, S. Haladyn, Pierre Tennstedt, Andreas Krüll, Adrian Münscher, Cordula Petersen, and A. Gröbe
Subjects: medicine.medical_specialty, business.industry, Head and neck cancer, Locally advanced, Hematology, medicine.disease, Surgery, Oncology, Radiology Nuclear Medicine and imaging, medicine, Radiology, Nuclear Medicine and imaging, In patient, Treatment time, business
Published: 2015
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278. 40(th) EASD Annual Meeting of the European Association for the Study of Diabetes : Munich, Germany, 5-9 September 2004

Author: S. Artigas, A V Dreval, Mark I. McCarthy, C Watson, Peter H. Bennett, M Quint, Y Ikeda, E Alpert, F Schiele, H Sekihara, Erik Gylfe, P Lowe, J Kuhlmann, Alain Golay, V Longo, Shahidul Alam Khan Akm., L G Mantovani, M Zawodniak-Szalapska, G Winkler, T Harrity, L Virág, U Johne, Kuo S-W., Linda C Tapsell, J Rodriguez, Michel Komajda, K Kankova, Carole A. Cull, M Sporna, E Estilles, U Ribel, M C Spruce, E Buzzigoli, T Prazak, J K McLaughlin, M K Lingohr, M Lim, F Calara, A Siebenhofer, G Meregalli, Roberto Anichini, A D Baron, R Kurashvili, P C Butler, G I Fantus, T. E. De Gooyer, Park Y-M., R. Walther, S Heinrich, Agnieszka Zawiejska, S Mukherjee, Nikolaos Papanas, G Wong, Ian D. Caterson, David M. Maahs, Shuichi Kaneko, Alexandra E. Butler, Francisco Javier Ampudia-Blasco, O N Kong, Attali J-R., C A Hedman, K Oshinyemi, Nicolle Müller, I C Cranston, N Okumus, M V Vlaiculescu, Balasubramanian Ravikumar, W W Cheatham, K Mukasa, K B Biswas, Annunziata Lapolla, Phil McEwan, G Mader, Gilles Chassot, Dragi Anevski, Werner A. Scherbaum, M Donath, C Hesselmann, R A Gandhi, David E. Moller, Ezio Bonifacio, C Garcia, V Ifandi, P Hornnes, Nieuwenhoven Fav., C Puech, S Pérez-Del-Pulgar, Kim S-R., G Hines, C Rubio Terrés, Michael Gaster, N. Hosszufalusi, A Scholze, Andrew A. Young, Stavros Liatis, F Hariri, S Tan, Paul Valensi, Allan E. Karlsen, J Kim, E. Moberg, J Kaiser, L Berman, G Nelson, A Altkrüger, P Kothare, D B Cook, S Doran, G. van Dijk, Shahnaz Shahinfar, Kim C-S., P Stahl, M Manousaki, S Sigrist, S K Lim, M. P. Stern, A Guberti, C Rezzani, J McKenney, Karl Thomaseth, Sofia Carlsson, M Julia, R Brillante, I Rubesova, T Darkow, E Matsumoto, Wendy M. 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Fuller, Nicholas Katsilambros, Michele Muggeo, Pia Ekbom, Piero Marchetti, V Melki, C Bailleau, H Stavrianos, A D'Errico, Geremia B. Bolli, Amabile Maier, Kelter A-R., Anders Green, Q J Morélis, Steffen Thiel, C Watkins, R C Cheung, A Clark, Elvira Fioriti, N Ari, Nam J-Y., Y Cottin, L G Krinelke, H Al Mohammedi, Simon C. Fleming, C Jones, Z Kerényi, Ahn Y-H., Meile M-J., P Nánási, M Graner, V Canonico, Gangnerau M-N., Hugh R. Taylor, Giovanni Sartore, A. Dejgaard, Carol Kelley, S. Ali, Stéphane Dalle, Jeffrey S. Gonzalez, Elena Šeböková, Alexander Beck, Ingo B. Leibiger, M Rosu, C Pencea, Werner Waldhäusl, Kaltenbacher M-C., R Butzer, S Thore, Adam G. Tabak, Angelo Avogaro, E Standi, Boris Kovatchev, O Bradescu, Patrizia Dentelli, A Fujita, C Verri, R Chlup, Prasad Ydm., S V Hörsten, van der Merwe M-T., D Hilliard, W Klein, D Worthley, M Udvardy, Berit R. 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Jensen, G, Fisher, A, Petrovsky, N, Srikusalanukul, W, Budge, M, Trifunovic zamaklar, D, Zivkovic, M, Jelic, V, Vukomanovic, G, Ristic, A, Seferovic, P, Costa, J, Duarte, S, Manley, S, Sailesh, S, Venkataraman, A, Haider, Y, Groza, I, Oprean, M, Ardelean, A, Morosanu, A, Darkow, T, Vanderplas, A, Mamas, M, Mcelduff, P, Burns, J, Edwards, R, Fitchet, A, Young, R, Gibson, J, Lichiardopol, R, Niculescu, N, Totora, A, Pencea, C, Tomescu, I, Cinteza, M, Manicardi, V, Coscelli, C, Navazio, A, Catellani, E, Michelini, M, Dall'Asta, D, Guberti, A, Piazza, A, Gasparini, E, Pantaleoni, M, Guiducci, U, Manari, A, Sejil, S, Janand delenne, B, Avierinos, J, Habib, G, Labastie, N, Vague, P, Lassmann vague, V, Luźniak, P, Tatoń, J, Wojciechowska Luźniak, A, Zairis, M, Lyras, A, Patsourakos, N, Tsirimbis, V, Foussas, S, Lupón, J, Urrutia, A, Herreros, J, González, B, Coll, R, Altimir, S, Prats, M, Valle, V, Abreu padí, C, Rábago, G, Ivanova, L, Brasacchio, D, Harno, E, Keenan, A, Li, H, Lu, Z, Ke, L, Liu, H, Jeong, I, Chae, M, Choi, M, Yoo, H, Kim, C, Yun, M, Na, M, Kang, Y, Kong, O, Son, S, Kim, I, Tanaka, N, Hosoi, M, Matsuyama, Y, Fukumoto, M, Yamakita, T, Yoshioka, K, Ishii, T, Sato, T, Fujii, S, Aoki, T, Shibata, T, Mizutani, N, Suzuki, J, Fowelin, J, Samuelsson, P, Brandrup wogsen, G, Okumura, K, Tokmakova, A, Staroverova, D, Antcieferov, M, Shutichina, I, Kuntchevich, G, Vriesendorp, T, Morélis, Q, Legemate, D, Schaper, F, Mainas, E, Gkioulmpasanis, I, Panagiotou, I, Vassilikos, G, Skorda, L, Sidira, M, Christoforidou, M, Alaveras, A, Artikis, V, Evdemon, E, Lechleitner, M, Koch, T, Ebenbichler, C, Sturm, W, Moretti, L, Moruzzo, D, Boldrini, E, Pandolfo, C, Kameyama, M, Iwasa, R, Cho, M, Nam, J, Huh, K, Kaplar, M, Paragh, G, Erdei, A, Csongradi, E, Garai, I, Varga, J, Galuska, L, Udvardy, M, Higa, M, Kaneko, Y, Hiroi, N, Koziarska, D, Nowacki, P, Majkowska, L, Luzniak, P, Wojciechowska luźniak, A, Tushuizen, M, Nieuwland, R, Snoeck, D, Sturk, A, Diamant, M, Aguiar, L, Bahia, L, Villela, N, Laflor, C, Conde, C, Bottino, D, Dorigo, D, Bouskela, E, Pu, S, Luo, Z, Lam, K, Dan, Q, Xu, A, Shen, J, Cheng, K, Xu, J, Thamer, C, Stefan, N, Haap, M, Heller, E, Tschritter, O, De Prado, A, Ortiz, A, Ybarra, J, Gich, I, Pou, J, Ehren, M, Roggenland, D, Reinsen, B, Klein, H, Rittig, K, Stock, J, Kocher, B, Balletshofer, B, Shon, H, Chung, D, Nakatani, Y, Matsuhisa, M, Kaneto, H, Hatazaki, M, Yoshiuchi, K, Katakami, N, Kawamori, D, Ohtoshi, K, Sakamoto, K, Matsuoka, T, Ozawa, K, Ogawa, S, Hori, M, Yamasaki, Y, Zitouni, K, Harry, D, Nourooz zadeh, J, Earle, K, Olesen, P, Franco, L, Corvaja, C, Semplicini, A, Ceylan işık, A, Arı, N, Rösen, P, Lee, I, Park, K, Jung, E, Shin, D, Jo, S, Obuobie, K, Prakash, P, Hanna, F, Lazarus, J, Varadhan, L, Gurushankar, J, James, D, Sheikh, S, Gaede, P, Zou, D, Vilarrasa, N, Perez maraver, M, Mena, E, Perez, D, Setti, G, Buckingham, R, Urbančič, V, Stefanovska, A, Bernjak, A, Ažman juvan, K, Kocijančič, A, Glowania, A, Filters, T, Fosmark, D, Torjesen, P, Kilhovd, B, Berg, T, Sandvik, L, Hanssen, K, Mentink, C, Donchenko, G, Stepanenko, S, Maingrette, F, Deng, H, Lindenmair, A, Freudenthaler, A, Baumgartner parzer, S, Nizheradze, K, Khoruzhenko, A, Tronko, N, Sheu, W, Ou, H, Shen, H, Lin, T, Wu, H, Yang, C, Mogylnytska, L, Schmoelzer, I, Davies, J, Band, M, Struthers, A, Prázný, M, Škrha, J, Kasalová, Z, Neelotpol, S, Jahan, P, Kauschke, S, Harrop, C, Schäfer, A, Widder, J, Eigenthaler, M, Walter, U, Uchimura, I, Ikebukuro, M, Kaibara, M, Hirata, M, Helal, R, Pervin, F, Yang, X, Jansson, P, Nagaev, I, Jack, M, Carvalho, E, Sunnerhagen, K, Cam, M, Cushman, S, Smith, U, Creely, S, Farmer, J, Gustafson, B, Kusminski, C, Krusinova, E, Wohl, P, Klementova, M, Lanska, V, Mcdougall, C, Kelly, I, Abbas, Z, Lutale, J, Archibald, L, Karunajeewa, H, Stingemore, N, Stuccio, G, Mcgechie, D, Muller, L, Hak, E, Goudzwaard, W, Montorsi, F, Homering, M, Sprenger, K, Goldstein, I, Asnaghi, V, Ferrari, G, Rastaldi, M, Gabellini, D, Dell'Antonio, G, Maestroni, A, Ruggieri, D, Luzi, L, Piemonti, L, Zerbini, G, Anafaroglu, I, Tutuncu, N, Sultana, M, Siddiqua, N, Iwasaki, T, Nakajima, A, Yoneda, M, Mukasa, K, Tanaka, S, and Sekihara, H
Subjects: 0303 health sciences, medicine.medical_specialty, business.industry, EASD, Endocrinology, Diabetes and Metabolism, Human physiology, medicine.disease, 03 medical and health sciences, 0302 clinical medicine, Diabetes mellitus, Family medicine, Internal Medicine, Medicine, business, 030217 neurology & neurosurgery, 030304 developmental biology
Published: 2004

279. Virtual planning of complex head and neck reconstructions - satisfactory match between real outcomes and virtual models

Author: Hanken, Henning, Smeets, Ralf, Al Dam, Ahmad, Blessmann, Marco, Vorwig, Oliver, Riecke, Björn, Heiland, Max, and Gröbe, Alexander
Subjects: ddc: 610, 610 Medical sciences, Medicine
Abstract: Purpose: The reconstruction of large facial bony defects using microvascular transplants requires extensive surgery to achieve full rehabilitation of form and function. This retrospective study evaluates the virtual planning tool ProPlan CMF®in 30 consecutive cases to enhance precision and efficiency[for full text, please go to the a.m. URL], 51. Jahrestagung der Deutschen Gesellschaft für Plastische und Wiederherstellungschirurgie (DGPW)
Published: 2013

280. Structural basis of substrate conversion in a new aromatic peroxygenase: cytochrome P450 functionality with benefits

Author: Klaus, Piontek, Eric, Strittmatter, René, Ullrich, Glenn, Gröbe, Marek J, Pecyna, Martin, Kluge, Katrin, Scheibner, Martin, Hofrichter, and Dietmar A, Plattner
Subjects: Molecular Docking Simulation, Binding Sites, Cytochrome P-450 Enzyme System, Protein Conformation, Catalytic Domain, Protein Structure and Folding, Agrocybe, lipids (amino acids, peptides, and proteins), Polycyclic Aromatic Hydrocarbons, Crystallography, X-Ray, Oxidation-Reduction, Mixed Function Oxygenases, Substrate Specificity
Abstract: Aromatic peroxygenases (APOs) represent a unique oxidoreductase sub-subclass of heme proteins with peroxygenase and peroxidase activity and were thus recently assigned a distinct EC classification (EC 1.11.2.1). They catalyze, inter alia, oxyfunctionalization reactions of aromatic and aliphatic hydrocarbons with remarkable regio- and stereoselectivities. When compared with cytochrome P450, APOs appear to be the choice enzymes for oxyfunctionalizations in organic synthesis due to their independence from a cellular environment and their greater chemical versatility. Here, the first two crystal structures of a heavily glycosylated fungal aromatic peroxygenase (AaeAPO) are described. They reveal different pH-dependent ligand binding modes. We model the fitting of various substrates in AaeAPO, illustrating the way the enzyme oxygenates polycyclic aromatic hydrocarbons. Spatial restrictions by a phenylalanine pentad in the active-site environment govern substrate specificity in AaeAPO.
Published: 2013

281. Lack of evidence for increased risk of postoperative bleeding after cutaneous surgery in the head and neck in patients taking aspirin

Author: Max Heiland, Lan Kluwe, Alexander Gröbe, and Wolfgang Eichhorn
Subjects: Male, medicine.medical_specialty, Dermatologic Surgical Procedures, Postoperative Hemorrhage, Surgical Flaps, Risk Factors, Medicine, Humans, In patient, Head and neck, Aged, Retrospective Studies, Aspirin, business.industry, Skin Transplantation, Mohs Surgery, Surgery, Increased risk, Otorhinolaryngology, Anticoagulant therapy, Ambulatory Surgical Procedures, Head and Neck Neoplasms, Anesthesia, Female, Oral Surgery, Facial Neoplasms, business, Intermediate risk, Neck, Platelet Aggregation Inhibitors, medicine.drug
Abstract: Cutaneous surgery in the head and neck carries an intermediate risk of bleeding, so many surgeons stop anticoagulant treatment perioperatively despite the risk of cardiovascular events. We reviewed many procedures done in patients who were taking aspirin, and we did not find evidence of significantly increased postoperative bleeding compared with procedures in patients who were not taking aspirin (10/259=4% compared with 6/209=3%, p=0.6). Our results suggest that cutaneous surgery in the head and neck is safe for patients taking aspirin.
Published: 2013

282. Incidence of bisphosphonate-related osteonecrosis of the jaw in consideration of primary diseases and concomitant therapies

Author: Alexandre T, Assaf, Ralf, Smeets, Björn, Riecke, Eva, Weise, Alexander, Gröbe, Marco, Blessmann, Tim, Steiner, Johannes, Wikner, Reinhard E, Friedrich, Max, Heiland, Frank, Hoelzle, and Frank, Gerhards
Subjects: Male, Bone Density Conservation Agents, Diphosphonates, Incidence, Imidazoles, Humans, Bisphosphonate-Associated Osteonecrosis of the Jaw, Bone Neoplasms, Female, Middle Aged, Zoledronic Acid
Abstract: Since its first description by Marx in 2003, the etiology of bisphosphonate-related osteonecrosis of the jaw (BRONJ) is the subject of numerous scientific discussions for oral and maxillofacial surgeons. Many retrospective studies on its etiology and pathogenesis have been carried out to explain pathological mechanisms; most of them just take a close look at the issue of dosage and application. Recently, attempts have been made, to identify co-factors which might promote the development of BRONJ.The present study is based on data of 169 patients with osseous metastatic malignancies. All patients received intravenous bisphosphonate therapy. On the basis of medical history, malignancy, and primary treatment, the modality of bisphosphonate therapy, and existing comorbidities and medication were analyzed. The role of immunosuppressive drugs, influence of underlying diseases, and general factors such as age and gender were examined. The predictability of necrotic involvement, influenced by the underlying malignancy and its specific therapy, e.g. radiation and cytostatic therapy were analyzed and statistically evaluated.A total of 8.9% (n=15) of patients developed BRONJ. The average time between diagnosis of malignancy and BRONJ was 80 months. Nine patients suffered from breast cancer, five had prostate cancer and one renal cancer. Separation into stage and histological subtype did not show any significant correlation, nor did age or gender, to the occurrence of BRONJ. However statistical analysis did show a significant correlation concerning monocytostatic (p=0.0215) and triple-cytostatic therapy (p=0.0137). The majority of patients with BRONJ (60%) received a bisphosphonate therapy including zoledronate. Single application with one bisphosphonate was administered in 28 cases; 44 patients had a medical history of different use of bisphosphonate. Concomitant medication did not suggest possible correlation, nor did accompanying diseases, arterial hypertension (33.33%) or arterial microcirculatory disturbances (20%).The evaluation of our results is pioneering. The influence of cytostatics and combined therapy of cytotoxic drugs on the pathogenesis of BRONJ is demonstrated here statistically. We confirmed a drug- and dose-dependent occurrence of BRONJ. Further prospective studies should be performed to elucidate the role of tissue perfusion and oxygen saturation, and the influence of immunosuppressive drugs in relation to the occurrence of BRONJ, as well as on wound healing of initial lesions.
Published: 2013

283. Immunohistochemical and FISH analysis of EGFR and its prognostic value in patients with oral squamous cell carcinoma

Author: Wolfgang Eichhorn, Johannes Wikner, Alexander Gröbe, Yogesh K. Vashist, Max Heiland, Lan Kluwe, Guido Sauter, Ronald Simon, Ralf Smeets, Meike Fraederich, and Marco Blessmann
Subjects: Adult, Male, Cancer Research, Pathology, medicine.medical_specialty, Cell, Disease-Free Survival, Pathology and Forensic Medicine, Young Adult, Growth factor receptor, Gene duplication, medicine, Humans, EGFR Gene Amplification, Lymph node, In Situ Hybridization, Fluorescence, Aged, Neoplasm Staging, Aged, 80 and over, medicine.diagnostic_test, business.industry, Gene Amplification, Middle Aged, medicine.disease, Prognosis, Head and neck squamous-cell carcinoma, Immunohistochemistry, ErbB Receptors, Survival Rate, medicine.anatomical_structure, Ki-67 Antigen, Otorhinolaryngology, Head and Neck Neoplasms, Lymphatic Metastasis, Carcinoma, Squamous Cell, Periodontics, Female, Mouth Neoplasms, Lymph Nodes, Oral Surgery, Neoplasm Grading, business, Fluorescence in situ hybridization
Abstract: Objectives To study immunohistochemical expression of the epithelial growth factor receptor (EGFR) in oral carcinomas and the head and neck region to examine possible associations with various features of the tumors and survival of the patients. Materials and methods Sections were made from two tissue arrays composed of 206 oral squamous cell carcinomas and 427 squamous cell carcinomas of the head and neck region, respectively, and examined for EGFR expression and Ki-67 labeling index by means of immunohistochemistry, and for EGFR gene amplification by means of fluorescence in situ hybridization. Correlation between resulting parameters and with clinical features was evaluated using chi-square test and Kaplan–Meyer analysis. Results A statistically significant association was observed for strong EGFR immunohistochemical (IHC) expression with advanced lymph node involvement (P = 0.02). EGFR immunohistochemical expression did not significantly correlate with patient disease specific (DS) or overall survival (OS). EGFR gene amplification was not correlated with any of the tumor features nor to survival of the patients (DS and OS). Discussion Epithelial growth factor receptor IHC expression and gene amplification might be suitable to predict locoregional control in oral squamous cell carcinoma patients but an inappropriate predictor for patients survival.
Published: 2013

284. Fractal dimension of time-resolved autofluorescence discriminates tumour from healthy tissues in the oral cavity

Author: Marco Blessmann, Lan Kluwe, Carola Gerich, Georg Salomon, Henning Hanken, Alexander Gröbe, Jürgen Schreiber, Max Heiland, Jan Klatt, Jörg Opitz, and Publica
Subjects: Pathology, medicine.medical_specialty, Time Factors, Early detection, Oral cavity, Complete resection, Fractal dimension, Sensitivity and Specificity, Fluorescence, Oral carcinomas, medicine, Humans, Early Detection of Cancer, business.industry, Lasers, Optical Imaging, Area under the curve, Mouth Mucosa, Patient survival, Autofluorescence, Fractals, Spectrometry, Fluorescence, Otorhinolaryngology, Area Under Curve, Carcinoma, Squamous Cell, Surgery, Mouth Neoplasms, Oral Surgery, business, Forecasting
Abstract: Early detection and complete resection of oral carcinomas is of crucial importance for patient survival. This could be significantly improved by developing a non-invasive, sensitive and real-time detection technique. Time-resolved autofluorescence measurement is state-of-the-art technology originally developed for non-destructive inspection of material. In this study, we measured time-resolved autofluorescence in tumours and healthy tissues of the oral cavity ex vivo and calculated the corresponding fractal dimension which was significantly higher in tumours than in healthy tissues (1.8 vs. 1.6, P
Published: 2013

285. Preparation of labeled human drug metabolites and drug-drug interaction-probes with fungal peroxygenases

Author: Marzena, Poraj-Kobielska, Jens, Atzrodt, Wolfgang, Holla, Martin, Sandvoss, Glenn, Gröbe, Katrin, Scheibner, and Martin, Hofrichter
Subjects: Pharmaceutical Preparations, Molecular Probes, Deuterium Exchange Measurement, Humans, Drug Interactions, Agaricales, Hydroxylation, Mixed Function Oxygenases
Abstract: Enzymatic conversion of a drug can be an efficient alternative for the preparation of a complex metabolite compared with a multi-step chemical synthesis approach. Limitations exist for chemical methods for direct oxygen incorporation into organic molecules often suffering from low yields and unspecific oxidation and also for alternative whole-cell biotransformation processes, which require specific fermentation know-how. Stable oxygen-transferring biocatalysts such as unspecific peroxygenases (UPOs) could be an alternative for the synthesis of human drug metabolites and related stable isotope-labeled analogues. This work shows that UPOs can be used in combination with hydrogen/deuterium exchange for an efficient one-step process for the preparation of 4'-OH-diclofenac-d6. The scope of the reaction was investigated by screening of different peroxygenase subtypes for the transformation of selected deuterium-labeled substrates such as phenacetin-d3 or lidocaine-d3. Experiments with diclofenac-d7 revealed that the deuterium-labeling does not affect the kinetic parameters. By using the latter substrate and H2 (18) O2 as cosubstrate, it was possible to prepare a doubly isotope-labeled metabolite (4'-(18) OH-diclofenac-d6). UPOs offer certain practical advantages compared with P450 enzyme systems in terms of stability and ease of handling. Given these advantages, future work will expand the existing 'monooxygenation toolbox' of different fungal peroxygenases that mimic P450 in vitro reactions.
Published: 2013

286. Malignant hyperphenylalaninaemia—Current status (June 1977)

Author: Danks, D. M., Bartholomé, K., Clayton, B. E., Curtius, H., Gröbe, H., Kaufman, S., Leeming, R., Pfleiderer, W., Rembold, H., and Rey, F.
Published: 1978
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287. Morquio syndrome: Clinical findings in 11 patients with MPS IVA and 2 patients with MPS IVB

Author: Holzgreve, W., Gröbe, H., von Figura, K., Kresse, H., Beck, H., and Mattei, J. F.
Published: 1981
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288. Sanfilippo type C disease: Clinical findings in four patients with a new variant of mucopolysaccharidosis III

Author: Bartsocas, C., Gröbe, H., van de Kamp, J. J. P., von Figura, K., Kresse, H., Klein, U., and Giesberts, M. A. H.
Published: 1979
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289. The tay syndrome (congenital ichthyosis with trichothiodystrophy)

Author: Happle, R., Traupe, H., Gröbe, H., and Bonsmann, G.
Published: 1984
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290. Fatal pneumopathy after cytostatic treatment for leukemia in children: Report of two cases

Author: Müller, K. -M., Menne, R., Hüther, W., and Gröbe, H.
Published: 1979
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291. Hyperphenylalaniaemia due to dihydropteridine reductase deficiency

Author: Gröbe, H., Bartholome, K., Milstien, S., and Kaufman, S.
Published: 1978
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292. Homocystinuria (cystathionine synthase deficiency). Results of treatment in late-diagnosed patients

Author: Gröbe, H.
Published: 1980
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293. Calcified cerebral necrosis following ALL therapy

Author: Müller, K. -M., Menne, R., Bachmann, K. D., and Gröbe, H.
Published: 1981
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294. Vacuolated lymphocytes in type II glycogenosis —A diagnostic approach?

Author: Bassewitz, D. B. v., Bremer, H. J., Bourgeois, M., Gröbe, H., and Stoermer, J.
Published: 1977
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295. Postoperative bleeding risk for cutaneous surgery in the head and neck region with continued phenprocoumon therapy

Author: Alexander Gröbe, Ahmed Al-Dam, Lan Kluwe, Ralf Smeets, Max Heiland, Marco Blessmann, Wolfgang Eichhorn, Evgeny Barsukov, and Marc Eichhorn
Subjects: Severe bleeding, Male, medicine.medical_specialty, Blood transfusion, medicine.medical_treatment, Dermatologic Surgical Procedures, Aftercare, Nose, Postoperative Hemorrhage, Risk Assessment, Surgical Flaps, Phenprocoumon, Lesion, medicine, Humans, Blood Transfusion, International Normalized Ratio, Head and neck, Aged, Aged, 80 and over, Bleeding episodes, Hematoma, business.industry, Age Factors, Anticoagulants, Plastic Surgery Procedures, Mohs Surgery, Bandages, Hemostasis, Surgical, Surgery, medicine.anatomical_structure, Otorhinolaryngology, Anticoagulant therapy, Ambulatory Surgical Procedures, Head and Neck Neoplasms, Anesthesia, Female, Oral Surgery, medicine.symptom, business, medicine.drug, Follow-Up Studies
Abstract: In a total of 171 surgical procedures for lesions in the head and neck region in patients in whom phenprocoumon therapy was not stopped, 16 (9%) postoperative bleeding events were observed over a follow-up period of two weeks. Local measures were sufficient in all cases except one severe case where blood transfusion was needed and anticoagulant treatment was stopped for 7 days. The bleeding risk was significantly higher for the surgical procedures of the nose than those in other areas (21% versus 6%, P = 0.014), but was not influenced by the international normalized ratio (INR) of blood coagulation, size, site and type of the lesion, surgical procedure, and sex and age of the patients. The bleeding rate in patients not on any anticoagulation therapy was significantly lower (6/211 = 3%). Across both groups, just over 80% of the bleeding episodes were within the first two days (55% on the same day and 32% on the next day) of the surgery. No bleeding was recorded after 5 days. Our data suggest that cutaneous surgery in the head and neck region can be safely performed with continued phenprocoumon therapy in most cases in an INR range of 1.3–3.4, but rarely severe bleeding does occur and can be managed with a close-contact follow-up and with 24-h on call services during the first two days postoperatively.
Published: 2013

296. Ischämiebedingte Schäden myokutaner Transplantate: eine Untersuchung im Göttinger Minipig

Author: Eichhorn, W, Wehrmann, M, Smeets, R, Heiland, M, Blessmann, M, Kluwe, L, Al Dam, A, Hanken, H, and Gröbe, A
Subjects: ddc: 610, 610 Medical sciences, Medicine
Abstract: Einleitung und Fragestellung: Nach vier bis sechs Stunden globaler Ischämie treten irreversible Schäden an den Strukturen Muskulatur, Subkutis, Dermis und Epidermis in unterschiedlicher Ausprägung auf. In der vorliegenden Studie soll lichtmikroskopisch untersucht werden, welche Strukturen[for full text, please go to the a.m. URL], 51. Jahrestagung der Deutschen Gesellschaft für Plastische und Wiederherstellungschirurgie (DGPW)
Published: 2013
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297. Proteome analysis of human CD56neg NK cells reveals a homogeneous phenotype surprisingly similar to CD56dim NK cells.

Author: Voigt, Jenny, Malone, David F. G., Dias, Joana, Leeansyah, Edwin, Björkström, Niklas K., Ljunggren, Hans‐Gustaf, Gröbe, Lothar, Klawonn, Frank, Heyner, Maxi, Sandberg, Johan K., and Jänsch, Lothar
Abstract: Abstract: NK cells lacking CD56 (CD56neg) were first identified in chronic HIV‐1 infection. However, CD56neg NK cells also exist in healthy individuals, albeit in significantly lower numbers. Here, we provide an extensive proteomic characterisation of human CD56neg peripheral blood NK cells of healthy donors and compare them to their CD56dim and CD56bright counterparts. Unbiased large‐scale surface receptor profiling clustered CD56neg cells as part of the main NK cell compartment and indicated an overall CD56dim‐like phenotype. Total proteome analyses of CD56neg NK cells further confirmed their similarity with CD56dim NK cells, and revealed a complete cytolytic inventory with high levels of perforin and granzyme H and M. In the present study, twelve proteins discriminated CD56neg NK cells from CD56dim NK cells with nine up‐regulated and three down‐regulated proteins in the CD56neg NK cell population. Those proteins were functionally related to lytic granule composition and transport, interaction with the extracellular matrix, DNA transcription or repair, and proliferation. Corroborating these results, CD56neg NK cells showed modest cytotoxicity, degranulation, and IFN‐ɣ secretion as compared to CD56dim NK cells. In conclusion, CD56neg NK cells constitute functionally competent cells sharing many features of bona fide CD56dim NK cells in healthy individuals, but with some distinct characteristics. [ABSTRACT FROM AUTHOR]
Published: 2018
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298. A hematogenous spread brain abscess invading the right damaged temporomandibular joint

Author: Klatt, J., Heiland, M., Gröbe, A., Westphal, M., Schmelzle, R., and Pohlenz, P.
Published: 2012
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299. Radiochemische Bestimmung von Formaldehyd in Fasermaterial

Author: Rösseler, M., Paul, D., and Gröbe, A.
Published: 1971
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300. The clinical findings in a patient with nonketotic hyperglycinemia

Author: Reploh, H., Gröbe, H., Diekmann, L., Palm, D., Bassewitz, D. B. v., and Jenett, W.
Published: 1973
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