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Ripening of fleshy fruits involves complex physiological, biochemical, and molecular processes that coincide with various changes of the fruit, including texture, color, flavor, and aroma. The processes of ripening are controlled by ethylene in climacteric fruits and abscisic acid (ABA) in non-climacteric fruits. Increasing evidence is also uncovering an essential role for polyamines (PAs) in fruit ripening, especially in climacteric fruits. However, until recently breakthroughs have been made in understanding PA roles in the ripening of non-climacteric fruits. In this review, we compare the mechanisms underlying PA biosynthesis, metabolism, and action during ripening in climacteric and non-climacteric fruits at the physiological and molecular levels. The PA putrescine (Put) has a role opposite to that of spermidine/spermine (Spd/Spm) in cellular metabolism. Arginine decarboxylase (ADC) is crucial to Put biosynthesis in both climacteric and non-climacteric fruits. S-adenosylmethionine decarboxylase (SAMDC) catalyzes the conversion of Put to Spd/Spm, which marks a metabolic transition that is concomitant with the onset of fruit ripening, induced by Spd in climacteric fruits and by Spm in non-climacteric fruits. Once PA catabolism is activated by polyamine oxidase (PAO), fruit ripening and senescence are facilitated by the coordination of mechanisms that involve PAs, hydrogen peroxide (H2O2), ABA, ethylene, nitric oxide (NO), and calcium ions (Ca2+). Notably, a signal derived from PAO5-mediated PA metabolism has recently been identified in strawberry, a model system for non-climacteric fruits, providing a deeper understanding of the regulatory roles played by PAs in fleshy fruit ripening.

Summary: Phototheranostics integrates deep-tissue imaging with phototherapy (containing photothermal therapy and photodynamic therapy), holding great promise in early diagnosis and precision treatment of cancers. Recently, second near-infrared (NIR-II) fluorescence imaging exhibits the merits of high accuracy and specificity, as well as real-time detection. Among the NIR-II fluorophores, organic small molecular fluorophores have shown superior properties in the biocompatibility, variable structure, and tunable emission wavelength than the inorganic NIR-II materials. What's more, some small molecular fluorophores also display excellent cytotoxicity when illuminated with the NIR laser. This review summarizes the progress of small molecular NIR-II fluorophores with different central cores for cancer phototheranostics in the past few years, focusing on the molecular structures and phototheranostic performances. Furthermore, challenges and prospects of future development toward clinical translation are discussed.

Objective: Growing evidence suggests that sleep disturbance is a risk factor for Alzheimer's disease (AD). Amyloid-β (Aβ) deposition in the brain is a main pathophysiology of AD. Considering that peripheral Aβ level is associated with brain Aβ deposition, the present study investigated the relationship between sleep disturbance and plasma Aβ levels.Methods: This is a population-based cross-sectional study. A total of 1,459 participants from a village in the suburbs of Xi'an, China, were enrolled from January 3, 2017 to March 26, 2017. Sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI), and a PSQI score of 10 points as poor sleep quality. Cognitive function was assessed with the Mini-Mental State Examination (MMSE). Fasting venous blood was taken in the morning, and the plasma Aβ levels were measured using ELISA. The relationships between plasma Aβ levels and sleep quality were analyzed using multiple linear regression.Results: Among the participants, 231 had poor sleep quality (15.83%). The log-transformed Aβ40 level had significant differences among the different sleep groups (F = 3.216, p = 0.040). The log-transformed Aβ40 level was higher in the poor sleep quality group than that in the general sleep quality group [87.17 (73.42, 107.34) vs. 89.69 (74.81, 125.79) pg/ml, p = 0.016]. In bivariate analysis, sleep quality was negatively associated with the log-transformed plasma Aβ40 level (β = −0.025, p = 0.011).Conclusion: In the community population, poorer sleep quality is associated with a higher plasma Aβ40 level. This indicated that sleep disturbance might also involve in dysfunction of peripheral Aβ clearance.

Exploring urban travel patterns can analyze the mobility regularity of residents to provide guidance for urban traffic planning and emergency decision. Clustering methods have been widely applied to explore the hidden information from large-scale trajectory data on travel patterns exploring. How to implement soft constraints in the clustering method and evaluate the effectiveness quantitatively is still a challenge. In this study, we propose an improved trajectory clustering method based on fuzzy density-based spatial clustering of applications with noise (TC-FDBSCAN) to conduct classification on trajectory data. Firstly, we define the trajectory distance which considers the influence of different attributes and determines the corresponding weight coefficients to measure the similarity among trajectories. Secondly, membership degrees and membership functions are designed in the fuzzy clustering method as the extension of the classical DBSCAN method. Finally, trajectory data collected in Shenzhen city, China, are divided into two types (workdays and weekends) and then implemented in the experiment to explore different travel patterns. Moreover, three indices including Silhouette Coefficient, Davies–Bouldin index, and Calinski–Harabasz index are used to evaluate the effectiveness among the proposed method and other traditional clustering methods. The results also demonstrate the advantage of the proposed method.

In this paper, the multilevel classification model of high-speed railway signal equipment fault based on text mining technology is proposed for the data of high-speed railway signal fault. An improved feature representation method of TF-IDF is proposed to extract the feature of fault text data of signal equipment. In the multilevel classification model, the single-layer classification model was designed based on stacking integrated learning idea; the recurrent neural network BiGRU and BiLSTM were used as primary learners, and the weight combination calculation method was designed for secondary learners, and k-fold cross verification was used to train the stacking model. The multitask cooperative voting decision tree was designed to correct the membership relationship of classification results of each layer. Ten years of signal switch machine fault data of high-speed railway are used for experimental analysis; the experiment shows that the multilevel classification model can effectively improve the classification of signal equipment fault multilevel classification task evaluation index and can ensure the correctness of the subordinate relations’ classification results.

Aim: The objective of this research was to investigate the feasibility of transthoracic shear wave elastography in the differentiation of subpleural masses. Methods: Between December 2019 and November 2020,82 consecutive patients with radiographic evidence (including chest X ray and thoracic computed tomography CT) of single subpleural lesion enrolled in this research. The Young’s modulus E (including Emean and Emax) of each lesion was detected, and the Young’s modulus E of malignant lesions were compared with those of benign ones. We made diagnoses according to the results of pathology or standard clinical course for at least 3 months. Receiver operating characteristic (ROC) analysis was plotted to determine the cut-off point by maximizing the Youden index. Results: The Emean and Emax of the benign and malignant group was 34.68 ± 12.12 kPa vs. 53.82 ± 11.95 kPa (p < 0.001), 57.77 ± 14.45 kPa vs. 76.62 ± 17.04 kPa (p < 0.001). The ROC of Emean showed that when the cut-off point was 43.8 kPa, the Youden index (0.53) for distinguishing benign and malignant tumors was the largest (sensitivity 80.4 %, specificity 72.2 %, AUC = 0.848, p < 0.0001). When the cut-off point recommended by Emax ROC was 73.5 kPa, the Youden index (0.44) for distinguishing benign and malignant tumors was the largest (sensitivity 76.1 %, specificity 66.7 %, AUC = 0.780, p < 0.0001). Conclusions: This study demonstrated that we can employ transthoracic shear wave elastography as a valuable instrument in differentiating benign subpleural lesions from malign ones.

Transmission towers are prone to collapse under strong wind load, resulting in significant economic losses. In order to investigate the collapse mechanism and failure modes of the transmission tower under strong wind load and whether the wind vibration factor can greatly reflect the increasing effect of the fluctuating wind, the finite element method (FEM) is utilized to analyze the ultimate bearing capacity of a typical 220 kV transmission tower. The results show that the collapse of the tower under strong wind loads is usually due to the buckling of the leg members. When the reference wind speed is equal to 27 m/s, a small part of the main leg members reaches their yield strength, while the diagonal members are still in the elastic range, and the deformation of the transmission tower is unapparent at this wind speed. When reference wind speed is equal or greater than 30 m/s, the growing variety of main legs is totally into the plastic yield stage, and the overall deformation of this tower is visible. Therefore, the transmission tower is collapsed due to the large deformation caused by the elastic-plastic buckling of leg members. Based on the aforementioned study, a finite element model involving three transmission towers and four span transmission lines is established to analyze the dynamic response of the tower-line system below fluctuating wind. Results show that the wind-induced coefficients designed by current code not only notably satisfy the stress response of tower components subjected to fluctuating wind loads in the elastic phase but also accurately assess the collapse displacement of the transmission tower. The increasing effect of displacement on the top tower under fluctuating wind, unfortunately, could not considerably reply with the investigated factor, and the load-carrying capacity of the transmission tower in the plastic phase can be overestimated by static calculation results.

Aqueous CdTe quantum dots solar cells have been successfully fabricated by the blade coating method on the magnesium zinc oxide (Zn1−xMgxO or ZMO) window layer. Compared with the ZMO mono-window layer, the ZMO/CdS bi-window layer can decrease the interface recombination effectively due to the lower lattice mismatch and fast interdiffusion between CdS and CdTe. Moreover, the high temperature annealing of the CdTe quantum dots absorbed layer passivates the grain boundary of the CdTe crystalline via the replacement reaction of tellurium with sulfur. Finally, the conversion efficiency of our aqueous CdTe quantum dots solar device is improved from 3.21% to 8.06% with the introduction of the CdS interlayer and high temperature CdCl2 annealing. Our aqueous CdTe quantum dots solar devices show a large open circuit voltage and fill factor which are comparable with the conventional devices that are fabricated with organic CdTe quantum dots. We believe that it is the spike-like conduction band alignment between the ZMO and CdTe absorbed layer that reduces the majority carrier concentration, leading to the decrease in interface recombination probability.

In order to achieve a reasonable balance between the high electrical conductivity and high tensile strength of TiB2 particles (TiB2p) and TiB whiskers (TiBw) reinforced copper matrix composites prepared by in situ reaction casting, the effects of reaction temperature and heat treatment time on the electrical conductivity and tensile strength of composites were studied. The results show that the electrical conductivity increased while the tensile strength decreased with the increase in reaction temperature from 1300 °C to 1500 °C. With the increase in heat treatment time, the electrical conductivity increased, while the tensile strength of the composites increased first and then decreased. The electrical conductivity and tensile strength of composites are related to the degree of in situ reaction at liquid or solid state, and the reasons for the improved performance are also discussed. Finally, two optimized preparation processes are proposed by cooperative controlling the reaction temperature and heat treatment time, the comprehensive properties of (TiB2p+TiBw)/Cu composites are 79.6% IACS/603 MPa and 83.7% IACS/602 MPa, respectively.

Taxi demand forecasting plays an important role in ride-hailing services. Accurate taxi demand forecasting can assist taxi companies in pre-allocating taxis, improving vehicle utilization, reducing waiting time, and alleviating traffic congestion. It is a challenging task due to the highly non-linear and complicated spatial-temporal patterns of the taxi data. Most of the existing taxi demand forecasting methods lack the ability to capture the dynamic spatial-temporal dependencies among regions. They either fail to consider the limitations of Graph Neural Networks or do not efficiently capture the long-term temporal dependencies. In this paper, we propose a Spatial-Temporal Diffusion Convolutional Network (ST-DCN) for taxi demand forecasting. The dynamic spatial dependencies are efficiently captured through a two-phase graph diffusion convolutional network where the attention mechanism is introduced. Moreover, a novel temporal convolution module is designed to learn various ranges of temporal dependencies, including recent, daily, and weekly periods. Inside the module, convolution layers are stacked to handle very long sequences. Experimental results on two large-scale real-world taxi datasets from New York City (NYC) and Chengdu demonstrate that our method significantly outperforms seven state-of-the-art baseline methods.

ObjectivesRecent studies regarding the relationships between plasma amyloid-β (Aβ) levels and cognitive performance had inconsistent results. In this study, we aimed to characterize the relationship between cognitive decline and plasma Aβ levels in a large-sample cognitively normal population.MethodsThis population-based, prospective cohort study included 1,240 participants with normal cognition. The Mini-Mental State Examination (MMSE) was used to assess cognitive function at baseline and 2 years later. Restricted cubic splines, multivariate logistic regression, and multivariate linear regression models were used to evaluate the type of relationship between cognitive decline during the 2-year follow-up period and plasma Aβ levels (Aβ40, Aβ42, and Aβ42/40).ResultsParticipants with moderate Aβ40 levels had the highest risk of cognitive decline during a 2-year follow-up relative to individuals with low Aβ40 [odds ratio (OR): 0.60, 95% confidence interval (CI): 0.45–0.81, p < 0.001] or high Aβ40 (OR: 0.65, 95% CI: 0.49–0.87, p = 0.004) levels. The association between Aβ40 and cognitive decline did not depend on sex, education level, or APOE ε4 status. There was an interaction found between age (≤ 65 and > 65 years) and Aβ40 (p for interaction = 0.021). In individuals older than 65 years, there was a positive linear relationship between plasma Aβ40 and cognitive decline (OR: 1.02, 95% CI: 1.00–1.04, p = 0.027). For participants ≤ 65 years old, the lower Aβ40 and higher Aβ40 groups had a lower risk of cognitive decline than the medium Aβ40 group (OR: 0.69, 95% CI: 0.50–0.94, p = 0.02; OR: 0.63, 95% CI: 0.45–0.86, p = 0.004). None of relationship between plasma Aβ42, Aβ42/40 and cognitive decline was found during a 2-year follow-up.ConclusionThe relationship between plasma Aβ40 and cognitive decline was not linear, but an inverted-U shape in a cognitively normal population. The underlying mechanism requires further investigation.

Pioneer factors such as Zelda (Zld) help initiate zygotic transcription in Drosophila early embryos, but whether other factors support this dynamic process is unclear. Odd-paired (Opa), a zinc-finger transcription factor expressed at cellularization, controls the transition of genes from pair-rule to segmental patterns along the anterior-posterior axis. Finding that Opa also regulates expression through enhancer sog_Distal along the dorso-ventral axis, we hypothesized Opa’s role is more general. Chromatin-immunoprecipitation (ChIP-seq) confirmed its in vivo binding to sog_Distal but also identified widespread binding throughout the genome, comparable to Zld. Furthermore, chromatin assays (ATAC-seq) demonstrate that Opa, like Zld, influences chromatin accessibility genome-wide at cellularization, suggesting both are pioneer factors with common as well as distinct targets. Lastly, embryos lacking opa exhibit widespread, late patterning defects spanning both axes. Collectively, these data suggest Opa is a general timing factor and likely late-acting pioneer factor that drives a secondary wave of zygotic gene expression.

Abstract Polyamines are important for non‐climacteric fruit ripening according to an analysis of the model plant strawberry. However, the molecular mechanism underlying the polyamine accumulation during ripening has not been fully elucidated. In this study, an examination of our proteome data related to strawberry fruit ripening revealed a putative polyamine oxidase 5, FaPAO5, which was localized in the cytoplasm and nucleus. Additionally, FaPAO5 expression levels as well as the abundance of the encoded protein continually decreased during ripening. Inhibiting FaPAO5 expression by RNAi promoted Spd, Spm, and ABA accumulation while inhibited H2O2 production, which ultimately enhanced ripening as evidenced by the ripening‐related events and corresponding gene expression changes. The opposite effects were observed in FaPAO5‐overexpressing transgenic fruits. Analyses of the binding affinity and enzymatic activity of FaPAO5 with Spm, Spd, and Put uncovered a special role for FaPAO5 in the terminal catabolism of Spm and Spd, with a Kd of 0.21 and 0.29 µM, respectively. Moreover, FaPAO5 expression was inhibited by ABA and promoted by Spd and Spm. Furthermore, the RNA‐seq analysis of RNAi and control fruits via differentially expressed genes (DEGs) indicated the six most enriched pathways among the differentially expressed genes were related to sugar, abscisic acid, ethylene, auxin, gibberellin, and Ca2+. Among four putative PAO genes in the strawberry genome, only FaPAO5 was confirmed to influence fruit ripening. In conclusion, FaPAO5 is a negative regulator of strawberry fruit ripening and modulates Spm/Spd levels as a signaling event, in which ABA plays a central role.

Objective To investigate associations between left atrial diameter (LAD) and long-term outcomes in patients with anterior or non-anterior wall ST-elevation myocardial infarction (STEMI). Methods Patients with STEMI were included in this secondary analysis of data from a prospective cohort study in which the primary outcome was major adverse cardiovascular event (MACE) occurrence during a 2.5-year follow-up. A LAD cut-off value was obtained through receiver operating characteristic curve analysis. Kaplan-Meier curve and Cox regression analyses were applied. Subgroup Cox regression analysis was also performed, with patients stratified based on left ventricular diastolic diameter (LVEDD, > 55 mm and ≤55 mm). The relationship between LAD and outcomes in patients with anterior or non-anterior wall STEMI was explored using restricted cubic spline functions. Results Out of 464 patients, adjusted Cox regression showed that dichotomous (>40 mm) LAD was significantly associated with MACE (hazard ratio 2.978, 95% confidence interval 1.763, 5.030) in patients with anterior wall but not non-anterior wall STEMI. The association was not different between normal and enlarged LVEDD groups. Conclusions A left atrium > 40 mm may indicate higher risk of MACE in patients with anterior wall STEMI, even in patients with normal left ventricular structure. This relationship was not observed in patients with non-anterior wall STEMI.

In Eriocheir sinensis aquaculture, crabs feeding on an artificial diet without steroid hormones available in ice-fresh fish or snails show delayed ovarian maturation and late listing. Icariin (ICA), a safe plant-derived monomer compound, can effectively promote estradiol (E2) synthesis and ovarian development in mammals, but its effect in crustaceans has not been reported. We investigated the effects of ICA on estrogen synthesis, vitellogenesis, and oocyte development in Chinese mitten crabs fed with one of four diets containing 0, 50, 100, and 200 mg/kg ICA, respectively, for 8 weeks. Results showed that vitellogenin (VTG) contents, gonadosomatic indexes (GSIs), and oocyte volumes in ICA50 and ICA 100 crabs were significantly higher than those in the control. The highest upregulated expression of VTG in hepatopancreases and ovaries was detected in ICA100 crabs. Hemolymph E2 contents were also significantly increased in ICA100 crabs. Enzyme-linked immunosorbent assay (ELISA) and western blot analysis indicated that the increase in E2 concentrations was attributable to an elevation in aromatase protein levels in ovaries through the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA)/cAMP response element binding (CREB) protein pathway. These findings suggest that ICA could serve as a natural additive to resolve the problem of delayed ovarian maturation of crabs raised in ponds.

The goal of this study was to determine whether the plasma leptin, nesfatin-1, cortisol, brain-derived neurotrophic factor (BDNF), and inflammatory cytokines could be used as potential biomarkers for the degree of craving in the alcohol-dependent patients after 1 month of abstinence. A total of 83 patients with alcohol use disorder (AUD) and 61 healthy subjects were assessed. Patients with AUD were selected from Department of Material Dependence, Anhui Mental Health Center, and subjects in the control group were selected from healthy volunteers. The Alcohol Urge questionnaire Scale (AUQ) was used to evaluate the extent of craving for alcohol, and the Michigan Alcoholism Screening Test (MAST), the Fagerstrom Test for Nicotine Dependence (FTND), the Self-Rating Anxiety Scale (SAS), and the Self-Rating Depression Scale (SDS) were also assessed in patients with AUD. Enzyme-Linked Immunosorbent Assay (ELISA) was used for the measurement of plasma leptin, nesfatin-1, cortisol, BDNF, Interleukin-6 (IL-6), C-reactive protein (CRP), and tumor necrosis factor-α (TNF-α) levels. Compare with healthy controls, the average leptin, leptin/BMI, IL-6, CRP, and TNF-α levels in patients with AUD were significantly increased, while the BDNF levels were significantly decreased. Moreover, the partial correlational analysis showed that the AUQ scores of the alcohol-dependent patients were positively correlated with the plasma leptin levels (r = 0.613, P < 0.001), rather than nesfatin-1 (r = 0.066, P = 0.569) after controlling for age as covariate. Furthermore, plasma nesfatin-1 levels were found to be correlated with the SDS scores (r = 0.366, P = 0.001) in the AUD group. In addition, plasma leptin levels were positively associated with the plasma IL-6 (r = 0.257, P = 0.033), CRP (r = 0.305, P = 0.011), and TNF-α (r = 0.311, P = 0.009) levels, and negatively associated with the BDNF levels (r = −0.245, P = 0.042) in patients with AUD. These results suggest that plasma leptin, but not nesfatin-1, might be a potential biomarker for the degree of craving in alcohol-dependent patients after 1 month of abstinence, the mechanism of which might be related to the dysfunction of the inflammatory cytokines and BDNF levels.

Efficient parking tends to be challenging in most large cities in China. Drivers often spend substantial amounts of time looking for parking lots while driving at low speeds, thereby resulting in interference with road traffic. This paper focuses on efficiently allocating parking spaces to the demanders. A double-objective model is proposed that considers both the utilizing rate and the walking distance. First, managers want to utilize parking resources fully. Therefore, they tend to prioritize the efficient distribution of parking spaces in response to parking demands. However, demanders typically choose parking spaces according to convenience. The second objective is the acceptable walking distance from the parking space to the destination. The particle swarm optimization (PSO) algorithm is used to solve this model. We collected parking demand and supply data in a central business district (CBD) of Harbin in China and evaluated the feasibility of the model. The results demonstrate that the proposed model increases the occupying rates of parking lots in residential zones while decreasing the walking distance. The shared use of parking spaces maximizes the utility and alleviates the shortage of parking spaces in downtown.

Background. Emerging studies have explored the association between the famine exposure during early life and the risk of the metabolic syndrome, and the results remain controversial. This meta-analysis was performed to summarize the famine effects on the prevalence of metabolic syndrome (MetS) in adulthood. Materials and Methods. We searched the PubMed, Web of Science, Embase, ScienceDirect, and Chinese National Knowledge Infrastructure for relevant studies up to December 2019. Pooled odd ratios (ORs) with 95% confidence intervals (CIs) were used to estimate the effect exposure to famine on MetS using a random-effects model, and the I2 was used to evaluate the heterogeneity. Results. The analyses included 39 studies from 10 articles with a total of 81504 participants. Fourteen studies from 10 articles for fetal famine exposure, 20 studies from 7 articles for childhood famine exposure, and 5 studies from 3 articles for adolescence/adult famine exposure were included in this meta-analysis. Compared with a nonexposed group, famine exposure significantly increased the risk of MetS for early life famine exposure (OR=1.27, 95% CI: 1.18-1.38), fetal famine exposure (OR=1.27, 95% CI: 1.14-1.43), and childhood famine exposure (OR=1.29, 95% CI: 1.16-1.44). Subgroup analyses showed that the result was consistent regardless of the study designs, definitions of MetS, and causes of famine, with or without adjustment for age, smoking, drinking, and physical activity. Conclusions. This meta-analysis suggests that exposure to famine during early life may increase the risk of MetS in adulthood.

In this study, we investigate a physical mechanism to improve the light absorption efficiency of graphene monolayer from the universal value of 2.3% to about 30% in the visible and near-infrared wavelength range. The physical mechanism is based on the diffraction coupling of surface plasmon polariton resonances in the periodic array of metal nanoparticles. Through the physical mechanism, the electric fields on the surface of graphene monolayer are considerably enhanced. Therefore, the light absorption efficiency of graphene monolayer is greatly improved. To further confirm the physical mechanism, we use an interaction model of double oscillators to explain the positions of the absorption peaks for different array periods. Furthermore, we discuss in detail the emerging conditions of the diffraction coupling of surface plasmon polariton resonances. The results will be beneficial for the design of graphene-based photoelectric devices.

Positioning of spacecraft (e.g., geostationary orbit (GEO), high elliptical orbit (HEO), and lunar trajectory) is crucial for mission completion. Instead of using ground control systems, global navigation satellite system (GNSS) can be an effective approach to provide positioning, navigation and timing service for spacecraft. In 2020, China finished the construction of the third generation of BeiDou navigation satellite system (BDS-3); this global coverage system will contribute better sidelobe signal visibility for spacecraft. Meanwhile, with more than 100 GNSS satellites, multi-GNSS navigation performance on the spacecraft is worth studying. In this paper, instead of using signal-in-space ranging errors, we simulate pseudorange observations with measurement noises varying with received signal powers. Navigation performances of BDS-3 and its combinations with other systems were conducted. Results showed that, owing to GEO and inclined geosynchronous orbit (IGSO) satellites, all three types (GEO, HEO, and lunar trajectory) of spacecraft received more signals from BDS-3 than from other navigation systems. Single point positioning (SPP) accuracy of the GEO and HEO spacecraft was 17.7 and 23.1 m, respectively, with BDS-3 data alone. Including the other three systems, i.e., GPS, Galileo, and GLONASS, improved the SPP accuracy by 36.2% and 19.9% for GEO and HEO, respectively. Navigation performance of the lunar probe was significantly improved when receiver sensitivity increased from 20 dB-Hz to 15 dB-Hz. Only dual- (BDS-3/GPS) or multi-GNSS (BDS-3, GPS, Galileo, GLONASS) could provide continuous navigation solutions with a receiver threshold of 15 dB-Hz.

Abstract In this work, a modified separator coated with a functional layer of reduced graphene oxide (RGO) anchored by cerium oxide (CeO2) nanoparticles was developed. The superior conductivity of RGO and chemical immobilization of high-ordered sulfur-related species (mainly Li2S n 4 ≤ n ≤ 8) of CeO2 yielded batteries with enhanced characteristics. A remarkable original capacity of 1136 mAh g−1 was obtained at 0.1 C with capacity retention ratio of 75.7% after 100 charge/discharge cycles. Overall, these data indicate that the separator with CeO2/RGO composite is promising to suppress the shuttling of polysulfides for better utilization of the active material.

Abstract As one of the key members of the coxsackievirus B group, coxsackievirus B5 (CV-B5) can cause many central nervous system diseases, such as viral encephalitis, aseptic meningitis, and acute flaccid paralysis. Notably, epidemiological data indicate that outbreaks of CV-B5-associated central nervous system (CNS) diseases have been reported worldwide throughout history. In this study, which was conducted to promote CV-B5 vaccine and anti-virus drug research, a 3-day-old BALB/c mouse model was established using a CV-B5 clinical isolate (CV-B5/JS417) as the challenge strain. Mice challenged with CV-B5/JS417 exhibited a series of neural clinical symptoms and death with necrosis of neuronal cells in the cerebral cortex and the entire spinal cord, hindlimb muscles, and cardiomyocytes. The viral load of each tissue at various post-challenge time points suggested that CV-B5 replicated in the small intestine and was subsequently transmitted to various organs via viremia; the virus potentially entered the brain through the spinal axons, causing neuronal cell necrosis. In addition, this mouse model was used to evaluate the protective effect of a CV-B5 vaccine. The results indicated that both the inactivated CV-B5 vaccine and anti-CVB5 serum significantly protected mice from a lethal infection of CV-B5/JS417 by producing neutralizing antibodies. In summary, the first CV-B5 neonatal mouse model has been established and can sustain CNS infections in a manner similar to that observed in humans. This model will be a useful tool for studies on pathogenesis, vaccines, and anti-viral drug evaluations.

Summary: Transcriptome deconvolution in cancer and other heterogeneous tissues remains challenging. Available methods lack the ability to estimate both component-specific proportions and expression profiles for individual samples. We present DeMixT, a new tool to deconvolve high-dimensional data from mixtures of more than two components. DeMixT implements an iterated conditional mode algorithm and a novel gene-set-based component merging approach to improve accuracy. In a series of experimental validation studies and application to TCGA data, DeMixT showed high accuracy. Improved deconvolution is an important step toward linking tumor transcriptomic data with clinical outcomes. An R package, scripts, and data are available: https://github.com/wwylab/DeMixTallmaterials. : Computational Bioinformatics; Cancer; Transcriptomics Subject Areas: Computational Bioinformatics, Cancer, Transcriptomics

Neutralizing antibodies (NTAbs) is a major criterion for evaluation the immunogenicity of many vaccines, for example, poliovirus and EV71 vaccine. Here, we firstly discovered that polyclonal antibodies induced by inactivated CVA16 vaccine and lived CVA16 virus have poor ability to neutralize circulating CVA16 strains in vitro. However, the passive transfer of poorly neutralizing polyclonal antibodies can protect suckling mice from lethally challenged with circulating strains in vivo. In addition, the obvious dose response was found between the titer of antibodies and the survival rate. Interestingly, poorly neutralizing polyclonal antibodies against circulating CVA16 strains, have good ability to neutralize prototype strain G10 in vitro. Between G10 and circulating CVA16 strains, there are total 47 variant sites in capsid, which are near the interface of VP1, VP2, and VP3, and close to 2-fold axis. Based on the structure of CVA16, the obvious structural changes were observed in residue 213 of VP1 GH loop, residue 139 of VP2 EF loop, and residues 59, 182 and 183 of VP3 GH loop. What we found may provide a new sight for the development of CVA16 vaccine.

Background/Aims: Autophagy is essential for maintaining cellular homeostasis and the survival of terminally differentiated cells as neurons. In this study, we aim to investigate whether mitofusin 2, a mitochondrial fusion protein, mediates autophagy in cerebral ischemia/reperfusion (I/R) injury. Methods: Primary cultured neurons were treated with oxygen-glucose deprivation/reperfusion to mimic cerebral I/R injury in vitro. Autophagosomes were visualized upon TEM. Autophagy-markers were then detected to monitor autophagy by western-blot and real-time PCR, and the autophagic flux was tracked with a mRFP-GFP-LC3 construct by fluorescence as well as autophagy inhibitors and agonists. The up- and downregulation of Mfn2 were through transfecting a lentivirusexpression vector respectively. And neuronal injury was detected by cell counting kit and TUNEL assay. Results: Results showed I/R increased autophagosome formation and inhibited autolysosome degradation. Furthermore, use of autophagy related agents demonstrated that I/R injury was caused by insufficient autophagy and aggravated by impaired autophagic degradation. The results also indicated that mitofusin 2 could ameliorate I/R injury through increasing autophagosome formation and promoting the fusion of autophagosomes and lysosomes. In contrast, downregulation of mitofusin 2 aggravated the I/R injury by inhibiting autophagosome formation and the fusion of autophagosomes and lysosomes. Additionly, mitofusin 2 overexpression did not lead to autolysosome accumulation induced by I/R. Conclusions: In summary, this study explicitly demonstrated that mitofusin 2 could ameliorate I/R injury mainly through promoting autophagy, which represented a potential novel strategy for neuroprotection against cerebral I/R damage.

Coxsackievirus B5 (CV-B5) is associated with various human diseases such as viral encephalitis, aseptic meningitis, paralysis, herpangina, and hand, foot and mouth disease (HFMD). However, there is currently no effective vaccine against CV-B5.The seroepidemiologic characteristics of CV-B5 remained unknown. A cohort study was carried out in 176 participants aged 6–35 months from January 2012 to January 2014. The serum samples were collected and tested for CV-B5 neutralizing antibodies (NtAbs) four times during these two years. The confirmed enterovirus cases were recorded through the surveillance system, and their throat or rectal swabs were collected for pathogen detection. According to the changes of CV-B5 NtAbs, two CV-B5 epidemics were detected among these participants during the two-year follow-up. Sixty-seven cases out of all participants had seroconversion in CV-B5 NtAbs. During the first epidemic from March 2012 to September 2012, CV-B5 seropositivity rate increased significantly (6.8%, 12/176 vs. 21.6%, 38/176, P = 0.000). The seroconversion rate and geometric mean fold-increase (GMFI) were 18.2% (32/176) and 55.7, respectively; During the second epidemic from September 2012 to January 2014, CV-B5 seropositivity rate also increased (21.6%, 38/176 vs. 38.6%, 68/176, P = 0.000), and the seroconversion rate and GMFI were 19.9% (35/176) and 46.5, respectively. Only one case had CV-B5 associated HFMD during the two-year follow-up, and CV-B5 from the throat swab isolate was GI.D3 subtype, which belonged to the major pandemic strain in mainland China. CV-B5 infection was common in infants and children in Jiangsu province, China. Therefore, it's necessary to strengthen the surveillance on CV-B5 and to understand the epidemic characteristics of CV-B5 infection.

Explosive ordnance disposal (EOD) robots can replace humans that work in hazardous environments to ensure worker safety. Thus, they have been widely developed and deployed. However, existing EOD robots have some limitations in environmental adaptation, such as a single function, slow action speed, and limited vision. To overcome these shortcomings and solve the uncertain problem of bomb disposal on the firing range, we have developed an intelligent bomb disposal system that integrates autonomous unmanned aerial vehicle (UAV) navigation, deep learning, and other technologies. For the hardware structure of the system, we design an actuator constructed by a winch device and a mechanical gripper to grasp the unexploded ordnance (UXO), which is equipped under the six-rotor UAV. The integrated dual-vision Pan-Tilt-Zoom (PTZ) pod is applied in the system to monitor and photograph the deployment site for dropping live munitions. For the software structure of the system, the ground station exploits the YOLOv5 algorithm to detect the grenade targets for real-time video and accurately locate the landing point of the grenade. The operator remotely controls the UAV to grasp, transfer, and destroy grenades. Experiments on explosives defusal are performed, and the results show that our system is feasible with high recognition accuracy and strong maneuverability. Compared with the traditional mode of explosives defusal, the system can provide decision-makers with accurate information on the location of the grenade and at the same time better mitigate the potential casualties in the explosive demolition process.

To study the relationship between β-carotene synthesis and geranylgeranyl pyrophosphate synthase (GGPS) activity, 15 species of Dunaliella were used to determine the changes in photosynthetic pigment contents, chlorophyll fluorescence parameters, β-carotene content, and GGPS activity. By observing the morphology and size of 15 species of Dunaliella, D8 has the largest individual algal cell and D9 has the smallest individual. Growth was relatively slow during days one through seven. After about eight days, the cells entered the logarithmic growth period and grew rapidly to a high density. After about 45 days, they entered a mature period, and growth slowed down. The contents of chlorophyll, carotenoids, and β-carotene increased during growth. D1 has the highest accumulation of β-carotene, and GGPS enzyme activity has a positive linear relationship with the β-carotene synthesis content. Phylogenetic analysis showed that the GGPS proteins of the 15 species were highly homologous, and the GGPS protein was not part of the membrane.

Spatial autocorrelation and skewed distribution are the most frequent issues in crash rate modelling analysis. Previous studies commonly focus on the spatial autocorrelation between adjacent regions or the relationships between crash rate and potentially risky factors across different quantiles of crash rate distribution, but rarely both. To overcome the research gap, this study utilizes the spatial autoregressive quantile (SARQ) model to estimate how contributing factors influence the total and fatal-plus-injury crash rates and how modelling relationships change across the distribution of crash rates considering the effects of spatial autocorrelation. Three types of explanatory variables, i.e., demographic, traffic networks and volumes, and land-use patterns, were considered. Using data collected in New York City from 2017 to 2019, the results show that: (1) the SARQ model outperforms the traditional quantile regression model in prediction and fitting performance; (2) the effects of variables vary with the quantiles, mainly classifying three types: increasing, unchanged, and U-shaped; (3) at the high tail of crash rate distribution, the effects commonly have sudden increases/decrease. The findings are expected to provide strategies for reducing the crash rate and improving road traffic safety.

AIM: To investigate the early effects of 3g/L sodium hyaluronate eye drops combined with soft contact lenses on corneal epithelial healing and local comfort. METHODS: Totally 90 patients(90 eyes)with primary monocular pterygium were randomly divided into three groups after pterygium excision surgeries(n=30, for each). Each group received pterygium excision combined with limbal stem cell autograft transplantation. The research group wore soft corneal contact lens for 7d after the surgery, and the next day all of them were given 3g/L sodium hyaluronate and levofloxacin eye drops four times a day, tobrmycin and dexamethasone ophthalmic ointment once every night. The Group B did not wear the corneal contact lens, they were given levofloxacin eye drops four times a day and tobrmycin and dexamethasone ophthalmic ointment once every night. About the Group C, the 3g/L sodium hyaluronate eye drops was added on the basis of conventional medication of Group B. The time of corneal epithelial healing after surgery and the ocular pain score in different times were observed and contrasted. RESULTS: Mean pain score of Group A were significantly lower than Group B and C at 6h, the first day and the third day after operation(PP>0.05). The first day after the surgery, there were no eyes of corneal epithelium completely healed, but the complete healing rate of Group A were significantly higher than the other two group at the second day and the third day.(PCONCLUSION: Early use of 3g/L hyaluronate sodium eye drops combined with soft corneal contact lenses after pterygium excision surgeries will not only accelerate corneal epithelial wound healing, but also relieve local pain, improve ocular comfort significantly.

Summary: Alteration of corticostriatal glutamatergic function is an early pathophysiological change associated with Huntington’s disease (HD). The factors that regulate the maintenance of corticostriatal glutamatergic synapses post-developmentally are not well understood. Recently, the striatum-enriched transcription factor Foxp2 was implicated in the development of these synapses. Here, we show that, in mice, overexpression of Foxp2 in the adult striatum of two models of HD leads to rescue of HD-associated behaviors, while knockdown of Foxp2 in wild-type mice leads to development of HD-associated behaviors. We note that Foxp2 encodes the longest polyglutamine repeat protein in the human reference genome, and we show that it can be sequestered into aggregates with polyglutamine-expanded mutant Huntingtin protein (mHTT). Foxp2 overexpression in HD model mice leads to altered expression of several genes associated with synaptic function, genes that present additional targets for normalization of corticostriatal dysfunction in HD. : Hachigian et al. demonstrate that manipulating levels of the striatum-enriched transcription factor Foxp2 can either rescue or mimic HD-associated behaviors in vivo. They link Foxp2 to the post-developmental regulation of the structure and function of the corticostriatal synapse. Keywords: Huntington’s disease, Foxp2, striatum, corticostriatal synapse

Flood is a kind of natural disaster that is extremely harmful and occurs frequently. To reduce losses caused by the hazards, it is urgent to monitor the disaster area timely and carry out rescue operations efficiently. However, conventional space observers cannot achieve sufficient spatiotemporal resolution. As spaceborne GNSS-R technique can observe the Earth’s surface with high temporal and spatial resolutions; and it is expected to provide a new solution to the problem of flood hazards. During 19–21 July 2021, Henan province, China, suffered a catastrophic flood and urban waterlogging. In order to test the feasibility of flood disaster monitoring on a daily basis by using GNSS-R observations, the CYGNSS (Cyclone Global Navigation Satellite System) Level 1 Science Data were processed for a few days before and after the flood to obtain surface reflectivity by correcting the analog power. Afterwards, the flood was monitored and mapped daily based on the analysis of changes in surface reflectivity from spaceborne GNSS-R mission. The results were evaluated based on the image from MODIS (Moderate Resolution Imaging Spectroradiometer) data, and compared with the observations of SMAP (Soil Moisture Active Passive) in the same period. The results show that the area with high CYGNSS reflectivity corresponds to the flooded area monitored by MODIS, and it is also in high agreement with SMAP. Moreover, CYGNSS can achieve more detailed mapping and quantification of the inundated area and the duration of the flood, respectively, in line with the specific situation of the flood. Thus, spaceborne GNSS-R technology can be used as a method to monitor floods with high temporal resolution.

Coastal Global Navigation Satellite System Reflectometry (GNSS-R) can be used as a valuable supplement for conventional tide gauges, which can be applied for marine environment monitoring and disaster warning. Incidentally, an important problem in dual-antenna GNSS-R altimetry is the crosstalk effect, which means that the direct signal leaks into the down-looking antenna dedicated to the reflected signals. When the path delay between the direct and reflected signals is less than one chip length, the delay waveform of the reflected signal is distorted, and the code-level altimetry precision decreases consequently. To solve this problem, the author deduced the influence of signal crosstalk on the reflected signal structure as the same as the multipath effect. Then, a simulation and a coastal experiment are performed to analyze the crosstalk effect on code delay measurements. The L5 signal transmitted by the Quasi-Zenith Satellite System (QZSS) from a geosynchronous equatorial orbit (GEO) satellite is used to avoid the signal power variations with the elevation, so that high-precision GNSS-R code altimetry measurements are achieved in the experiment. Theoretically and experimentally, we found there exists a bias in proportion to the power of the crosstalk signals and a high-frequency term related to the phase delay between the direct and reflected signals. After weakening the crosstalk by correcting the delay waveform, the results show that the RMSE between 23-h sea level height (SSH) measurements and the in-situ observations is about 9.5 cm.