646 results on '"Du Plessis, L"'
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252. Die loop van die dinge
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Du Plessis, L. J., primary
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- 1943
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253. Wat is in die Ekonomie eintlik Ekonomies?
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Du Plessis, L. J., primary
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- 1959
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254. A New Heat Exchange Unit
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Marchand, P., primary and Du Plessis, L., additional
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- 1964
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255. Die geloofsgrondslag van ons Republiek
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Du Plessis, L. J., primary
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- 1961
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256. A collection of Book Reviews for Volume 28, Issue 10
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Du Plessis, L. J., primary
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- 1961
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257. Die cachet-aandeel in die vorming van die Afrikanerdom
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Du Plessis, L. J., primary
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- 1962
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258. Socialistiese groeps-egoïsme as wesenskenmerk van ons verouderde Britse-Georiën-teerde SA vakbondswes
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Du Plessis, L. J., primary
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- 1958
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259. Ons verhouding tot Engeland en Amerika
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Du Plessis, L. J., primary
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- 1953
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260. Calvinistiese Perspektief: ’n Konstruktief bedoelde benadering van ons rassevraagstuk*
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Du Plessis, L. J., primary
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- 1960
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261. Calyn se betekenis vir die westerse kultuur ontwikkeling in die algemeen
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Du Plessis, L. J., primary
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- 1959
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262. Die groei van 'n Wes-Europese gemeenskap en van 'n gemeenskaplike regstel.
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Du Plessis, L. J., primary
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- 1959
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263. Uitwerking van ons nywerheidsonteikkeling op ons volkswese
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Du Plessis, L. J., primary
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- 1952
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264. Die nuwe Franse grondwet
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Du Plessis, L. J., primary
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- 1959
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265. Enkele Gedagtes oor die Calvinistiese Sosiologie en Geskiedenisfilosofie
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Du Plessis, L. J., primary
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- 1957
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266. Universiteit Kaapstad en die Calvinistiese staatsteorie: 'n Aankondigin en woord van waardering
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Du Plessis, L. J., primary
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- 1954
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267. Biochemical changes associated with the use of haemodilution with 5% dextrose in water and mannitol for open-heart surgery
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Obel, I. W. P., primary, Marchand, P., additional, and Du Plessis, L., additional
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- 1967
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268. The Anatomy of the Mitral Valve and its Associated Structures
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Du Plessis, L. A., primary and Marchand, P., additional
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- 1964
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269. Calvinisme en Marxisme*
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Du Plessis, L. J., primary
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- 1966
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270. Beskrywing van Afrika in 1641
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Du Plessis, L. J., primary
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- 1961
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271. Die landbou-arbeidskongres van Sabra.
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Du Plessis, L. J., primary
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- 1954
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272. Die Voortrekker-ideale in die moderne wêreld
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Du Plessis, L. J., primary
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- 1938
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273. 'n Valse Dilemma
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Du Plessis, L. J., primary
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- 1956
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274. Leemstes in Dooyeweerd se regsfilosofie
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Du Plessis, L. J., primary
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- 1958
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275. The Quantitative Treatment of Plateau Chromatograms Obtained with Complex-Forming Fixed Phases
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du Plessis, L. A., primary
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- 1963
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276. 'n Konstruktief bedoelde reaksie teen skadelike repristinasies in ons regsopvattings op die gebied van staat en kerk
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Du Plessis, L. J., primary
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- 1956
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277. Ons Suid-Afrikaanse regsteoretiese grondbegrippe gekonfronteer met die van Prof. Dooyeweerd
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Du Plessis, L. J., primary
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- 1957
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278. Calvinistiese reaksies op die huidige wêreldveroordeling van Suid-Afrika
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Du Plessis, L. J., primary
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- 1960
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279. Law, Language and the Multilingual State
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Brohy, Claudine, Turi, Joseph-G., Woehrling, José, and du Plessis, Lodewyk
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linguistic law ,language charters in Africa ,legal ,law ,multilingual state ,South Africa ,language rights ,bilingualism ,language barometer ,linguistic conviviality ,sociolinguistic principles ,linguistic autonomy ,bic Book Industry Communication::L Law::LB International law ,bic Book Industry Communication::C Language::CF linguistics - Abstract
The theme of the conference, “Language, Law and the Multilingual State”, was determined to investigate the state-juridical challenges facing multilingual societies. Several related issues were addressed, such as minority and indigenous languages, globalisation and diversity, language rights, language ideology and language legislation.
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- 2013
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280. What does an enabling environment for infant and young child nutrition look like at implementation level? Perspectives from a multi-stakeholder process in the Breede Valley Sub-District, Western Cape, South Africa.
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Du Plessis, L. M., McLachlan, M. H., and Drimie, S. E.
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INFANT nutrition , *CHILD nutrition , *OVERWEIGHT children , *CHILDREN'S health , *CHILDHOOD obesity - Abstract
Background: Breede Valley is a sub-district of the Cape Winelands district, Western Cape Province, South Africa. The administrative capital of the district is situated in the semi-rural town Worcester. Findings of a baseline survey in Worcester revealed poor infant feeding practices and childhood under- and overnutrition, with particular concern over high levels of stunting and low dietary diversity. Maternal overweight and obesity was high. These characteristics made the site suitable to study multi-sectoral arrangements for infant and young child nutrition (IYCN). The purpose of this study was to explore elements of an enabling environment with key stakeholders aimed at improving IYCN at implementation level.Methods: Focus group discussions and interviews were conducted with representatives from two vulnerable communities; local and district government; higher education institutions; business; and the media in the Breede Valley. Audio recordings were transcribed and data were analysed with the Atlas.TI software programme.Results: The participants viewed knowledge and evidence about the first 1000 days of life as important to address IYCN. The impact of early, optimal nutrition on health and intellectual development resonated with them. The IYCN narrative in the Breede Valley could therefore be framed around nutrition's development impact in a well-structured advocacy campaign. Participants felt that capacity and resources were constrained by many competing agendas spreading public resources thinly, leaving limited scope for promotion and prevention activities. "People" were viewed as a resource, and building partnerships and relationships, could bridge some shortfalls in capacity. Conversations about politics and governance elicited strong opinions about what should be done through direct intervention, policy formulation and legislation. A lead government agency could not be identified for taking the IYCN agenda forward, due to its complexity. Participants proposed it should be referred to a local, informal, inter-governmental body where directors and senior managers meet to address issues of cross-cutting importance.Conclusion: The study illustrated that knowledge and evidence; politics and governance; and capacity and resources, elements of the international definition of an enabling environment, also apply at implementation level. In addition, our findings indicated that a people-centred approach is critical in shaping the enabling environment at this level. [ABSTRACT FROM AUTHOR]- Published
- 2018
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281. SLE pachymeningitis and multiple cranial nerve palsies: a case report and review of the literature.
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John, T J, John, K, du Plessis, L, and Manie, M
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CRANIAL nerves , *DURA mater , *CRANIAL nerve diseases , *SYSTEMIC lupus erythematosus , *PARALYSIS , *RARE diseases - Abstract
Hypertrophic pachymeningitis (HP) is a relatively uncommon disease associated with focal or diffuse thickening of the dura mater secondary to underlying chronic inflammation. The link between systemic lupus erythematosus (SLE) and hypertrophic pachymeningitis (HP) is extremely rare, with only six other cases reported in the literature. We, however, report the first case of SLE pachymeningitis presenting with multiple cranial nerve palsies. The patient showed good response to steroids and cyclophosphamide therapy. One should maintain a high index of suspicion to make the diagnosis in patients with SLE presenting with neurological dysfunction. Prompt therapy prevents long-term neurological sequelae. [ABSTRACT FROM AUTHOR]
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- 2019
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282. MON-PP256: Missed and Used Opportunities in Health Status Assessment of Children.
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Blaauw, R., Daniels, L., Du Plessis, L., Koen, N., Koornhof, L., Marais, M., Nel, D., Van Niekerk, E., and Visser, J.
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- 2015
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283. Reducing the dimensionality of hyperspectral data using diffusion maps.
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du Plessis, L., Damelin, S., and Sears, M.
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- 2009
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284. Assessment of the viability of Saccharomyces cerevisiae in response to synergetic inhibition during bioethanol production.
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De Klerk, Corli, Fosso-Kankeu, Elvis, Du Plessis, L., and Marx, S.
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SACCHAROMYCES cerevisiae , *ETHANOL as fuel , *CELL survival , *LIGNOCELLULOSE , *FLOW cytometry - Abstract
Second-generation biofuels, fuels produced from lignocellulosic materials, including wood, agricultural residues and biomass waste include bioethanol, biodiesel and biogas. These fuel sources have great potential as useful substitutes to conventional fossil fuels. Biomass sources are also non-toxic and biodegradable energy sources that can be produced from a wide range of organic materials resulting in economic and renewable energy source. Pretreatment of lingocellulosic biomass is required to reduce physicochemical restrictions that hinder the accessibility of sugars necessary for hydrolysis and fermentation. Various pretreatment processes exist, but all of them produce inhibitory compounds that ultimately reduce ethanol production and cell viability of the fermenting microorganism, Saccharomyces cerevisiae. In this study different combinations of inhibitors (acetic acid, formic acid and vanillin) were considered to mimic realistic fermentation conditions during bioethanol production; ethanol yield and cell viability were then concurrently measured over a period of 48 h. The combination of acetic acid and formic acid exhibited ethanol reduction up to 11 ± 3.74%, while cell viability decreased by 23 ± 6.61%. Acetic acid and vanillin reduced ethanol production by 25 ± 1.77% and cell viability by 4 ± 4.38%. Formic acid and vanillin inhibited ethanol production by 31 ± 3.14% and cell viability 16 ± 7.54%. Finally, the synergistic effect of all three inhibitors reduced the final ethanol production by 58 ± 5.09% and cell viability by 27 ± 5.44%, indicating the toxic effect of the synergistic combination. [ABSTRACT FROM AUTHOR]
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- 2018
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285. Assessing the utilisation of a child health monitoring tool.
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Blaauw, R., Daniels, L., du Plessis, L. M., Koen, N., Koornhof, H. E., Marais, M. L., van Niekerk, E., and Visser, J.
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HEALTH status indicators , *CHILDREN'S health , *CHILD nutrition , *HEALTH promotion , *VITAMIN A in human nutrition - Abstract
Background. The Road-to-Health booklet (RtHB), a standardised national tool for growth monitoring and the assessment of health among children from birth to five years of age, was introduced in South Africa in February 2011. Objectives. The study assessed the implementation of growth monitoring and promotion, immunisation, vitamin A supplementation, and deworming sections of the RtHB. Caregivers' (CGs) and healthcare workers' (HCWs') knowledge, attitudes and practices were investigated as well as HCWs' perceptions of barriers undermining implementation. Methods. A cross-sectional descriptive study was conducted on a proportional sample of randomly selected primary healthcare facilities across six health districts (35%; n=143) in the Western Cape Province. HCWs involved in the implementation of the RtHB booklet, children (aged 0 - 36 months) and CGs were included. Information was obtained through scrutiny of the RtHB, observation of consultations and structured questionnaires. Results. A total of 2 442 children, 2 481 CGs and 270 HCWs were recruited. Weight measurements (94.7%, n=2 251/2 378) were performed routinely. Less than half (40.2%; n=997/2 481) of CGs reported that their child's growth had been explained to them. Sixty-eight percent of HCWs (n=178/260) correctly identified criteria for underweight classification, whereas only 55% (n=134/245) and 39% (n=95/245) could do so for stunting and wasting, respectively. The RtHB sections were completed adequately for immunisation (89.3%; n=2 171/2 431) and vitamin A supplementation (94.6%; n=1 305/1 379) but not for deworming (48.8%; n=176/361). Most HCWs (93%; n=209/223) knew the correct regimens for vitamin A supplementation, but few CGs knew when treatment was due for vitamin A supplementation (16.4%, n=409/1 646) and deworming (26.2%; n=650/2 481). Potential barriers identified related to inadequate training, staff shortages and limited time. Conclusion. Focused efforts and resources should be channelled towards HCWs' training and monitoring regarding growth monitoring and promotion to optimise utilisation of the RtHB. Mobilisation of community health workers is needed to strengthen community awareness of preventive health interventions. [ABSTRACT FROM AUTHOR]
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- 2017
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286. Palm olein quality parameter changes during industrial production of potato chips
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du Plessis, L. M. and Meredith, A. J.
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New legislation introduced in South Africa for the quality of used frying oils has resulted in the need to identify quicker, more suitable methods that correlate well with results from two official methods, namely, total polymerized glycerides and total polar components. Oil and product samples were taken at regular intervals during a commercial frying process in palm olein. Oil samples were analyzed for a number of different quality parameters viz. tocopherol content, dielectric constant, total polymerized glycerides, total polar components, tertiary butylhydroquinone (TBHQ) content, anisidine value, Rancimat induction period, and free fatty acid content, and the results statistically compared to results from official methods. Oil was expressed from product stored under accelerated conditions and analyzed for the same quality parameters. Fried product was also subjected to sensory evaluation to measure the degree of oil deterioration and sensory preference. The frying trial was successfully executed with refined, bleached, and deodorized palm olein and the frying oil used to a free fatty acid (FFA) content of 0.41%. Oil and product sampling were done at different FFA value levels. Frying oil quality was verified at the onset of the trial and at regular intervals. The frying oil total polar component value increased to approximately half of the limit set by the official regulation. This point was reached mainly due to the high starting value of the fresh oil. Frying oil total polymerized glycerides increased from below 1% to 2.1%. This increase is negligible when compared to the general trend for polyunsaturated oils. The alternative laboratory methods used for predicting oil quality can be rated as follows: total tocopherol content >dielectric constant >FFA >TBHQ content >anisidine value >Rancimat induction period. The first three methods correlated well with total polar component levels and it is recommended that the dielectric constant and FFA measurements be applied for monitoring oil condition during frying. It is possible that viscosity changes could be used for the monitoring of polyunsaturated frying oils. Evaluation of oil extracted from product revealed a negligible effect of non-oil components on oil quality parameters. The same was observed when product was stored at −10°C and at 37°C.
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- 1999
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287. The diurnal patterns of ruminal enzymatic activity and in vitro digestibility of starch, neutral detergent fiber, and protein.
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Raffrenato, E., Badenhorst, M.J., Harvatine, K.J., Shipandeni, M.N.T., du Plessis, L., Esposito, G., and van Zyl, W.H.
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STARCH , *ENZYMATIC analysis , *COSINE function , *DAIRY cattle , *PROTEINS , *DESMOPRESSIN , *CELLULASE , *MILKFAT - Abstract
The objective of this study was to determine whether diurnal patterns in starch, neutral detergent fiber (NDF) and protein digestibilities and amylolytic, fibrolytic, and proteolytic activities exist in dairy cows. Rumen fluid was collected from 4 ruminally cannulated Holstein dairy cows before the morning feeding and subsequently every 4 h for a 24-h period. Two of the cows were restricted from feed for 8 h overnight, and the other 2 continued to receive their feed ad libitum, to isolate and quantify the effects of changes in feeding behavior at night. After 2 runs the cows were crossed over between night feeding treatments. Rumen fluid was analyzed for enzymatic activity and in vitro starch, NDF, and nitrogen digestibility. Circadian rhythm analyses of enzymatic activity and in vitro digestibility were conducted by fitting the linear form of a cosine function with a 24-h period. Patterns were observed in activity for amylase, lichenase, endoglucanase, and xylanase, with the highest activities observed at the time points subsequent to milking and feed delivery. Protease activity was unaffected by either feeding treatment or possible feeding behavior. When fitted to a cosine function, all the parameters tested followed a daily pattern that was sensitive to the overnight availability of feed, although the parameters responded differently to the feeding treatment. The patterns displayed by in vitro digestibility results of starch, NDF, and nitrogen, across the various fluid collection time points, were highly variable. The time at peak (acrophase) observed in the enzymatic analysis did not correspond to those observed in the in vitro analysis. These results suggest that different interpretations should be given to enzymatic activities and in vitro digestibility values, and the time of rumen fluid collection relative to feeding time should be considered and reported when rumen fluid is used for research or commercial purposes. Maximum digestibility appears in fact to be reached around 4 to 5 h after the main ration delivery for NDF and starch and around ration delivery for protein. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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288. ASSESSING NUTRITION INTERVENTION PROGRAMMES THAT ADDRESSED MALNUTRITION AMONG YOUNG CHILDREN IN SOUTH AFRICA BETWEEN 1994-2010.
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Iversen, P. O., Marais, D., du Plessis, L., and Herselman, M.
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MALNUTRITION , *NUTRITION , *CHILDREN , *HEALTH programs , *POVERTY , *MEDLINE - Abstract
Several nutrition intervention programmes have been implemented to alleviate malnutrition among young children in South Africa. However, both nationwide studies and smaller, region-based reports show that many of these programmes have failed to improve nutritional health among the target groups. This is an overview of the most important nutrition intervention programmes implemented in post-apartheid South Africa. It is based on data sampled between 1994 and 2010, and collected from HighWire Press and MEDLINE data bases, local journals, official reports as well as experience gathered over the past two decades. Abstracts and nutrition issues related to chronic and communicable and non-communicable diseases were not included. The main aim of the implemented supplementation programmes after the Second World War was to correct states of undernutrition. However, these efforts did not benefit the disadvantaged groups, particularly the coloureds and the blacks. Following the downfall of the apartheid regime, various nutrition intervention programmes have been implemented with varying degrees of success. These include health facility-based programmes, community-based programmes as well as nutrition promotion strategies. The more holistic approach of the Integrated Nutrition Programme since 1994 is commendable, but programmes have failed to restore adequate growth rates among the impoverished children. Most likely, the failures are not as a result of inappropriate policies and strategies or lack of knowledge about relevant solutions, but rather inadequate implementation and scale of the programmes. In order to ensure a satisfactory improvement in nutritional health in South Africa, the various programmes should undergo regular evaluations to identify pitfalls and shortcomings. The fight against undernutrition and hunger, which are rooted in poverty and social inequalities, remains a major challenge for the South African health authorities. Urgent scale-up of the current nutrition intervention programmes as well as evaluation of their implementation are needed, coupled with strategies for education, skills development, job creation and poverty alleviation. [ABSTRACT FROM AUTHOR]
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- 2012
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289. Evaluation of two nano-silane-modified emulsion stabilised pavements using accelerated pavement testing.
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Rust, F.C., Smit, M.A., Akhalwaya, I., Jordaan, G.J., and du Plessis, L.
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PAVEMENT testing , *ACCELERATED life testing , *EMULSIONS , *PAVEMENTS , *CONSTRUCTION materials , *SILANE - Abstract
Upgrading, maintenance and rehabilitation of road infrastructure is expensive, especially in view of the growing scarcity and cost of suitable road building materials. In areas with high mica content and secondary minerals such as smectite in the natural materials, stabilisation with cement is not viable. The Council for Scientific and Industrial Research of South Africa has embarked on a research programme to evaluate the performance of substandard materials improved with anionic nano-silane modified bitumen emulsions for use in base and subbase layers. This work comprises laboratory testing as well as Accelerated Pavement Testing using the Heavy Vehicle Simulator (HVS). The results of a full-scale HVS test on a light pavement as well as initial analysis on a medium traffic road are discussed. It has been shown that stabilisation of available substandard materials using an anionic nano-silane modified bitumen emulsion compared with the standard approach of importing high quality crushed aggregate can lead to savings as high as 40%–50% for equivalent performance. In addition, there was also a significant reduction in construction effort and time. [ABSTRACT FROM AUTHOR]
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- 2022
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290. Nemaline myopathy in a six-month-old Pomeranian dog.
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Bester, E. G., Kitshoff, A. M., Botha, W. J., van Wilpe, E., du Plessis, L., and Williams, J.
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NEMALINE myopathy , *MUSCLE weakness , *FORELIMB , *PERIPHERAL nervous system , *MUSCULAR atrophy , *AUTOPSY , *KEGEL exercises , *MUSCLE growth - Abstract
Nemaline myopathy – a clinically and genetically complex heterogenous group of disorders – is described uncommonly in humans and rarely in animals, and is characterised by progressive muscle weakness. The diagnosis is confirmed by histological and/or ultrastructural identification of subsarcolemmal, thread-like, rod-shaped structures called nemaline rod bodies within more than 40% of skeletal muscle fibres. These rods contain the Z-line protein, α-actinin, that can be effectively stained in skeletal muscles using Gomori or Masson trichrome and negatively stained with periodic acid-Schiff. Similar rod-like bodies have been found in smaller numbers in dogs with endocrine disorders and occasionally in other conditions in humans. This report is of a six-month)old Pomeranian dog which had progressive exercise intolerance over a two-month period associated with severe disuse muscle atrophy of the thoracic limbs, as well as gradual pelvic limb weakness and regurgitation of food. Baseline diagnostics ruled out endocrinopathies and after histological and ultrastructural evaluation of thoracic limb muscles and nerve biopsies confirmed nemaline myopathy. The clinical course, diagnostic test results, ultrastructure of skeletal muscle and peripheral nerve, gross necropsy findings and histopathology using various stains are described and illustrated. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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291. Identifying the critical success factors for South Africa as a business tourism destination
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Marais, Milandrie, Du Plessis, L, and Du Plessis, L.
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Critical success factors ,South Africa ,Destinations ,Tourism ,Business tourism ,Management - Abstract
MCom (Tourism Management), North-West University, Potchefstroom Campus, 2016 In addition to the growth and availability of business tourism in South Africa there is also an increase in the competition for South Africa as a business tourism destination. Business tourism is an indispensable part of the tourism industry and involves all trips related to business and work purposes. Business tourism and events are often also referred to as the MICE or MCE industry and events, the meetings segment, and the convention or conference industry. Business tourism holds ample advantages for a destination, such as decreasing seasonality, catalysing short-breaks, creating jobs, and increasing destination image. Furthermore, business tourists often spend more money than leisure tourists, thereby making a bigger economic impact per capita than leisure tourists. The importance of business travel and tourism in Africa was noted by Dieke (1998:39) as early as 1998. During 2015, 4.8% of foreign arrivals to South Africa represented business tourists. This figure was greater than the percentage of business tourists in 2014 – confirming growth in South Africa’s business tourism sector. This growth stresses the necessity of knowing what contributes to competitiveness and success within business tourism. Although both internal and external factors can contribute to success, managers in South Africa can manage, implement, and control the internal factors in an attempt to enhance business tourism. However, the critical success factors (CSFs) have to be determined in order to manage them. Therefore, the goal of this study was to determine the CSFs relevant to business tourism in South Africa. To achieve this goal, two articles were written: Article 1 analysed and reviewed CSFs in the tourism industry to gain a better understanding of this management approach, and Article 2 identified the CSFs for South Africa’s business tourism. These articles were preceded by Chapter 2: a literature study contextualising and analysing destinations and the tourism industry, the business tourism sector, and management. Previous studies on CSFs within the tourism industry were also identified and listed in Chapter 2. In Article 1 (Chapter 3) a total of 52 studies were identified and used for this review paper on CSFs. The ten most significant CSFs most frequently found (by including both statistical and descriptive analyses) were human resources, finances, customer / customer-related aspects, quality, facilities, effectiveness, marketing, systems, hygiene, and product. Three of the factors that appeared among the top five of both statistical and descriptive analyses were quality, finances, and human resources. Quality and facilities were two factors that appeared among the top five in both supply and demand approaches. Furthermore, quality appeared as an important CSFs through statistical and descriptive analyses as well as across supply and demand approaches. These results helped to design the interview guidelines for Article 2. The aim of Article 2 (Chapter 4) was to conduct a qualitative survey in order to identify the CSFs of business tourism in South Africa from the supply side. This was achieved by conducting structured interviews with seven respondents who are events/meeting coordinators within the business tourism sector in South Africa. The respondents were selected based on their willingness to participate. Four themes namely finances, human resources, product, and customer-related aspects, were identified. This is the first study focusing on the business tourism sector as a whole in South Africa, thereby contributing greatly to management. Research not only provided information regarding the CSFs for business tourism in South Africa, but it also provided findings regarding the industry as a whole and about the analyses of CSFs. Understanding the CSFs for business tourism can contribute to sustainable growth and competitiveness in the business tourism sector. Masters
- Published
- 2016
292. Genomic and epidemiological monitoring of yellow fever virus transmission potential.
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Faria, N. R., Kraemer, M. U. G., Hill, S. C., de Jesus, J. Goes, Aguiar, R. S., Iani, F. C. M., Xavier, J., Quick, J., du Plessis, L., Dellicour, S., Thézé, J., Carvalho, R. D. O., Baele, G., Wu, C.-H., Silveira, P. P., Arruda, M. B., Pereira, M. A., Pereira, G. C., Lourenço, J., and Obolski, U.
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YELLOW fever , *VIRAL transmission , *EPIDEMICS , *AEDES aegypti , *MOSQUITO vectors , *PUBLIC health surveillance - Abstract
The yellow fever virus (YFV) epidemic in Brazil is the largest in decades. The recent discovery of YFV in Brazilian Aedes species mosquitos highlights a need to monitor the risk of reestablishment of urban YFV transmission in the Americas. We use a suite of epidemiological, spatial, and genomic approaches to characterize YFV transmission. We show that the age and sex distribution of human cases is characteristic of sylvatic transmission. Analysis of YFV cases combined with genomes generated locally reveals an early phase of sylvatic YFV transmission and spatial expansion toward previously YFV-free areas, followed by a rise in viral spillover to humans in late 2016. Our results establish a framework for monitoring YFV transmission in real time that will contribute to a global strategy to eliminate future YFV epidemics. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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293. Clinical features and outcome of lupus myocarditis in the Western Cape, South Africa.
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Du Toit, R., Herbst, P. G., van Rensburg, A., du Plessis, L. M., Reuter, H., and Doubell, A. F.
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MYOCARDITIS , *MORTALITY & race , *SYSTEMIC lupus erythematosus , *STRESS echocardiography , *LUPUS nephritis , *LYMPHOPENIA , *PATIENTS , *DISEASE risk factors - Abstract
Background: African American ethnicity is independently associated with lupus myocarditis compared with other ethnic groups. In the mixed racial population of the Western Cape, South Africa, no data exists on the clinical features/outcome of lupus myocarditis. Objectives: The objective of this study was to give a comprehensive description of the clinical features and outcome of acute lupus myocarditis in a mixed racial population. Methods: Clinical records (between 2008 and 2014) of adult systemic lupus erythematosus (SLE) patients at a tertiary referral centre were retrospectively screened for a clinical and echocardiographic diagnosis of lupus myocarditis. Clinical features, laboratory results, management and outcome were described. Echocardiographic images stored in a digital archive were reanalysed including global and regional left ventricular function. A poor outcome was defined as lupus myocarditis related mortality or final left ventricular ejection fraction (LVEF) < 40%. Results: Twenty-eight of 457 lupus patients (6.1%) met inclusion criteria: 92.9% were female and 89.3% were of mixed racial origin. Fifty-three per cent of patients presented within three months after being diagnosed with SLE. Seventy-five per cent had severely active disease (SLE disease activity index ≥ 12) and 67.9% of patients had concomitant lupus nephritis. Laboratory results included: lymphopenia (69%) and an increased aRNP (61.5%). Treatment included corticosteroids (96%) and cyclophosphamide (75%); 14% of patients required additional immunosuppression including rituximab. Diastolic dysfunction and regional wall motion abnormalities occurred in > 90% of patients. LVEF improved from 35% to 47% (p = 0.023) and wall motion score from 1.88 to 1.5 (p = 0.017) following treatment. Overall mortality was high (12/28): five patients (17.9%) died due to lupus myocarditis (bimodal pattern). Patients who died of lupus myocarditis had a longer duration of SLE (p = 0.045) and a lower absolute lymphocyte count (p = 0.041) at diagnosis. LVEF at diagnosis was lower in patients who died of lupus myocarditis (p = 0.099) and in those with a persistent LVEF < 40% (n = 5; p = 0.046). Conclusions: This is the largest reported series on lupus myocarditis. The mixed racial population had a similar prevalence, but higher mortality compared with other ethnic groups (internationally published literature). Patients typically presented with high SLE disease activity and the majority had concomitant lupus nephritis. Lymphopenia and low LVEF at presentation were of prognostic significance, associated with lupus myocarditis related mortality or a persistent LVEF < 40%. [ABSTRACT FROM AUTHOR]
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- 2017
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294. Allelic variation in the promoter region of the LDL receptor gene: analysis of an African-specific variant in the FP2 cis-acting regulatory element
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Hoogendijk, C.F., Scholtz, C.L., Pimstone, S.M., Ehrenborg, E., Kastelein, J.J P., Defesche, J.C., Thiart, R., du Plessis, L., de Villiers, J.N.P., Zaahl, M.G., Delport, R., Rubinsztein, D.C., Raffel, L.J., Grim, C.E., Mediene-Benchekor, S., Amouyel, P., Brousseau, T., Steyn, K., Lombard, C.J., and Hayden, M.R.
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DNA , *LIPOPROTEINS , *ALLELES - Abstract
DNA samples of 2303 individuals from nine different population groups were screened for variant −175g→t in the promoter region of the low-density lipoprotein receptor (LDLR) gene. The −175g→t variant detected at carrier frequencies of 3–10% in different African population groups was absent in the Caucasian and Asian (Chinese) individuals studied. In contrast to previous findings in Black South Africans where this polymorphism predominated in patients with familial hypercholesterolaemia (FH), it occurred at a significantly lower frequency in hypercholesterolaemics from the recently admixed Coloured population of South Africa compared with population-matched controls
(P<0.0001). Haplotype and mutation analysis excluded the likelihood that this finding is due to association with a specific disease-related mutation in FH patients, although reversal of the positive association with FH observed in the Black population may, at least in part, be due to admixture linkage disequilibrium. Transient transfection studies in HepG2 cells demonstrated that the −175t allele is associated with a non-significant decrease (∼7%) of LDLR transcription in the absence of sterols. The data presented in this study raise the possibility that the −175g→t polymorphism may have subtle effects that become clinically important within certain genetic and/or environmental contexts. [Copyright &y& Elsevier]- Published
- 2003
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295. In vitro biocompatibility of transferosomes, ethosomes and transethosomes
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Volkwyn, Michelle, Du Plessis, L., Viljoen, J.M., 11948388 - Du Plessis, Lissinda Hester (Supervisor), 11320036 - Viljoen, Johanna Magdalena (Supervisor), and 11948388 - Du Plessis, Lissinda Hester (Supervisor)||11320036 - Viljoen, Johanna Magdalena (Supervisor)
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Lipid nanocarrier ,Convexity ,Characterization ,Malvern Morphologi G3 ,Circularity ,Elongation ,Image analysis - Abstract
MSc (Pharmaceutics), North-West University, Potchefstroom Campus Lipid nanocarriers (LN) for transdermal drug delivery, have gained more interest in recent years, due to their rapid penetration into the skin. Liposomes were investigated in the past with the goal of transdermal drug delivery, yet studies confirmed they were not able to achieve transdermal delivery, and should rather be considered for topical delivery. Focus moved to the ultradeformable lipid carriers due to their ability to penetrate the skin barrier without compromising the skin structure. Transferosomes are ultradeformable vesicles (UDV), which consist of a lipid and edge activator, and are the first generation of the elastic LN. Ethosomes are UDVs consisting of a lipid and ethanol, which acts as a membrane modulator, whereas transethosomes consist of a lipid and both an edge activator and ethanol. LN resemble cell organelles due to their dimensions and content, therefore, a risk of potential cytotoxicity occurs. The first step in determining the biocompatibility of these UDVs was to prepare and optimize LN formulations, including the UDVs (transferosomes, ethosomes and transethosomes) and liposomes as a control. After preparation of these vesicles, each system was characterized utilizing the standardized method of dynamic light scattering (DLS), measuring vesicle diameter, PDI and zeta potential. Quantitative image analysis, utilizing specific shape and size parameters have not been established for LN, due to being mainly used to characterize powder particles in the past. The size and shape parameters of each LN were established by means of image analysis with the Malvern Morphologi G3, including intensity mean, diameter of an equivalent circle (CE diameter), solidity, elongation, convexity, circularity and aspect ratio. The LN were fairly solid, and low levels of elongation were observed, as well as high levels of convexity. The circularity of the LN, however, were varied. It was concluded that elongation, convexity and circularity were parameters that could be utilized for characterization, complementary to DLS. The stability of each system was also observed for 90 days. The next step in determining the in vitro biocompatibility of the UDVs was to observe the effects they had on cells, by means of Thiazolyl blue tetrazolium bromide (MTT) and Trypan Blue dye exclusion assays, utilizing human malignant melanoma cells (A375) and primary epidermal keratinocytes (HaCat). Previous studies have suggested interference of the lipid content in liposomes with absorbance values as determined by the MTT assay, and it was investigated in this study whether this problem also occurred with UDVs. The effects of vesicle concentration on cell viability was investigated by means of MTT assay. A correlation between lipid content and high absorbance values was observed, therefore, confirming the interference of lipid content of the UDV with accurate cell viability results. The effects of treatment time on cell viability was also investigated, this time utilizing MTT, as well as Trypan Blue dye exclusion assay. No toxicity was observed for the A375 cells, even after the 48 h treatment period, however, cytotoxicity was observed when the HaCat cells were treated for periods longer than 48 h. Both the MTT and Trypan Blue method showed accurate results when determining cell viability, despite having different mechanisms through which they determine viability. The in vitro biocompatibility was therefore confirmed for the UDVs, although longer treatment periods may lead to cytotoxicity. Masters
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- 2019
296. Service quality management in conference tourism : expectations versus delivery
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McCallaghan, Wilhelmina Aletta, Slabbert, E., Du Plessis, L., and 10732586 - Slabbert, Elmarie (Supervisor)
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Vergaderings ,Conferences ,Konferensies ,Dienskwaliteit ,Verwagte diens ,Konferensie toerisme en Suid Afrika ,Meetings ,Werklike diens ,Service quality ,Perceived service ,Besigheidstoerisme ,Conference tourism and South Africa ,Expected service ,ComputingMilieux_MISCELLANEOUS ,Business tourism - Abstract
MCom (Tourism Management), North-West University, Potchefstroom Campus, 2016 The primary objective of this study was to determine service expectations of conference delegates in relation to the service delivered at Quest Conference Estate. This objective was achieved by firstly analysing service quality in terms of the expectation of service delivery prior to attending a conference and secondly analysing the actual service received whilst attending the conference and the influence thereof on the intention to return. Secondly, a literature study was conducted to review the role of service quality in the tourism industry, to identify and analyse current models that can be used to measure service quality (the SERVQUAL model was found to be the most applicable in the context of this study), to determine the service expectations and service perceptions of conference delegates at the Quest Conference Estate and to analyse the conference industry and the importance of service quality within. Thirdly the empirical results were discussed. Lastly, conclusions were drawn from the research and recommendations were made for the effective management of service delivery in a conference environment and for Quest Conference Estate, as well as for future research. From the literature study, research done by Parasuraman et al. was used as the theoretical framework for this study and the SERVQUAL model used for the exploratory phase. This model has been proven to be a valid and reliable measuring instrument of service quality and it was critical for the empirical research to be accurate and scientifically founded. The questionnaire was adapted to suit the needs of a conference service setting. The objective of the questionnaire was to measure the service quality of Quest Conference Estate. Questionnaires were given to 397 delegates who attended a conference, meeting or seminar at Quest Conference Estate. These delegates were asked to complete a section before the start of the conference on demographic information and what kind of service they expected to receive from Quest Conference Estate. The same delegates were approached after the conference, to indicate in another section of the questionnaire, their perception on the actual service received. The results were then statistically interpreted to determine the possible gap between expected service and actual service delivered. From the responses to the questionnaire an analysis on the expectations of visitors to Quest Conference Estate was done, and according to this, it was clear that the two main expectations from respondents were that the food and beverages service will be hygienic, adequate and sufficient and that the conference equipment will work properly. This was closely followed by the expectation that Quest Conference Estate will provide its guests with a safe and secure environment. It was also determined that most delegates attending a conference at Quest Conference Estate felt that they expected much more from the actual service received, but that they will still attend conferences at Quest Conference Estate in the future and will still recommend the facility to others. These results can be applied to bridge the gap between expected service and perceived service by offering the service that is actually expected from Quest Conference Estate. Most tourism businesses believe that they deliver good service but service delivery should be measured on a regular basis to determine whether it adheres to the needs of the delegates or not. Service delivery should also be offered at a consistent high level as delegates will in future expect the same and higher levels of service delivery as previously experienced. Masters
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- 2015
297. The development of a universal accessibility framework for national parks in South Africa and Zimbabwe
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Chikuta, Oliver, Du Plessis, L., Saayman, M., and 10201424 - Saayman, Melville (Supervisor)
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Universele toeganklikheid ,Raamwerk ,Natuurgebaseerde toerisme ,Disability ,SANParks ,Framework ,Gebreke ,Nature-based tourism ,ZimParks ,Universele ontwerp ,Universal design ,Universal accessibility ,SANParke ,Impairments ,Gestremdheid ,National parks ,Nasionale parke - Abstract
PhD (Tourism Management), North-West University, Potchefstroom Campus, 2015 Despite global growth in the disability tourism market, tourism organisations have not realised the full potential of this market due to a lack of understanding of this market segment as well as the perception that PwDs do not travel. Most facilities remain inaccessible to people with both permanent and temporary impairments. Disability movements (like the American Association of People with Disabilities, Aspies for Freedom and Handicap International) have raised their voices but not loud enough to stir action. Workshops, seminars and conferences have been held but most were talk shows with no practical implications. To date, no research-based universal accessibility framework exists for national parks in South Africa and Zimbabwe and no practical standards have been formulated to ensure accessibility to all in these parks. This study aimed to design a universal accessibility framework for national parks in South Africa and Zimbabwe. To achieve this goal one of the study's objectives was a literature review on universal accessibility and tourism. This objective is achieved in Chapter 2. The second objective was to establish the extent to which national parks in South Africa and Zimbabwe are universally accessible. Interviews were conducted with park management in both countries to meet this objective. The study also sought to establish the accessibility expectations of people with disabilities; this was attained by administering a 10-page online questionnaire to people with disabilities from across the globe. Finally, the study aimed to formulate a set of recommendations and develop a framework for universal accessibility. Using SPSS, Exploratory Factor Analysis and ANOVAs were performed in order to analyse and interpret data. Descriptive statistics were also used. The Alpha values and mean inter-item correlations confirmed the reliability and internal consistency of the data. Eleven factors were identified from the factor analysis: transport; parking and entrance; the reception area; restrooms; bird watching and game drives; trails; information accessibility; interpretation; water-based activities; accommodation and dining as well as campsites. The results revealed that while people with disabilities want all areas to be accessible, specific facilities were singled out in line with particular disabilities. People with mobility impairments were more worried about transport and parking than people with other impairments. They were also concerned about the accessibility of bird watching and game viewing as well as accommodation and dining than were people with hearing and visual impairments. People with hearing impairments placed much high value on the accessibility of the reception area than those with mobility and visual impairments. Interesting to note was the fact that people with visual impairments did not regard accessible restrooms as very important. Most importantly, all people with disabilities were strongly against steps and staircases in all facilities since they are a serious barrier to accessibility regardless of type of disability. They also attach more value to how they are treated when they visit national parks. This was identified as more important than physical access. A universal accessibility framework was developed based on the literature and research findings. This framework will go a long way in assisting policy makers to address accessibility issues in national parks and the tourism sector in general. Doctoral
- Published
- 2015
298. An analysis of management skills within graded establishments in South Africa
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Wessels, Walter Johan and Du Plessis, L.
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Gasvryheidsektor ,Hoër onderwys opleiding ,Bestuursvaardighede en opleiding van menslike hulpbronne ,Hoër onderwys opleiding ,Higher education and training curriculum ,Menslike hulpbronne ,Tourism industry ,Toerismebedryf ,Accommodation sector ,Human resources ,Hospitality sector ,Human capital ,Higher education ,Managerial skills ,Akkommodasie sektor ,Opvoeding ,Kurrikulum - Abstract
MA (Tourism Management), North-West University, Potchefstroom Campus, 2015 The tourism industry is one of the biggest economic contributors in South Africa and it is predicted that the industry will grow significantly over the years to come. This growth pressures South Africa to stay competitive and adhere to the needs of the visitors. The latter being one of the core functions of any tourism establishment. Labour structures in the tourism industry are constantly changing and to deliver quality products, employees should be well skilled and prepared for the demands of the industry. The accommodation sector, one of the biggest sectors in the tourism industry, provides a variety of services to tourists when visiting this country. It was determined in the literature review that the skills needed by an accommodation manager, to provide the expected services include flexibility, people skills, the ability to train other employees and the ability to explore the ever changing needs of the tourist. Higher education organisations in South Africa educate potential tourism employees in a variety of skills and knowledge. However, some employees are still of the opinion that these students are not adequately prepared for the demands of the industry and therefore complain. Added to this, there is very little communication between the industry and higher education organisations. Therefore the tourism industry feels that students are not employable which creates challenges for students, higher education organisations and the tourism industry. Education and skills development should contribute directly to the economic growth of South Africa and the government, a very important role player in the tourism industry, encourages education opportunities in South Africa. Therefore, the aim of this study is to determine, through industry participitation, what the management skills required within graded establishments in South Africa are. To achieve this aim, the following objectives were set: To analyse the literature available regarding the managerial theories, managerial levels, managerial processes and universal skills needed by managers, to analyse the tourism industry and hospitality sector, identify the important role played by managerial skills within this sector and to identify higher education programmes available in South Africa that focus specifically on managerial skills development for the accommodation sector. Thirdly, to collect and analyse the perceptions of accommodation owners and managers regarding the managerial skills and knowledge required by graduate managerial employees and then to draw conclusions and make recommendations to higher education organisations to adapt tourism management qualification programmes. This was achieved by making use of a quantitative research method by means of self-administered questionnaires, distributed to 254 Tourism Grading Counsil of South Africa’s (TGCSA) graded establishment managers in the nine (9) provinces of South Africa. The data, gathered from the questionnaire, was captured electronically by an online programme, SurveyMonkey. The data was then processed by means of SPSS and analysed. The descriptive results revealed that most of the responding accommodation establishments do employ graduates, but these graduates have to display certain characteristics. The top three characteristics for an accommodation manager included trustworthiness, responsibility and motivation in that order. Being ambitious, intelligent and sensitive rated the lowest. A factor analysis revealed that there are 10 important factors that contributes to being an effective accommodation manager. These skills included: 1) Personal characteristics, 2) Forecasting skills, 3) Strategic management skills, 4) Human resource skills, 5) Problem solving and crisis management skills, 6) Communication skills, 7) Information technology skills, 8) Customer service skills, 9) Financial skills and 10) Marketing skills. The comparisons (by means of ANOVA’s, t-tests and spearman rank order correlations) between skills needed by an accommodation manager and selected business characteristics (the province in which the accommodation establishment is located, the grading status, the size, the number of employees and the years that the accommodation establishment has been in operation) are unique to this study and various significant differences were identified. Comparisons have also been done between skills needed by an accommodation manager and selected personal characteristics (age, current position, duration of employment, level of higher education and the necessity of practical skills before employment of the respondent) which also revealed significant differences. The highest number of significant differences was identified for larger establishments, age, position of the respondents and the duration of employment. The results of this study can be used by higher education organisations to adapt their current tourism management qualifications, and by accommodation establishments to understand the training needs of employees. It did however voice the concerns of industry role players when it comes to new graduates and their employability. Masters
- Published
- 2015
299. Utilization of sunflower seed oil as a renewable fuel for diesel engines
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du Plessis, L
- Published
- 1981
300. A pharmacokinetic and efficacy study of Lumefantrine in mice: evaluating the application of Pheroid™ technology
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Govender, Katya, Wiesner, L, and Du Plessis, L
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InformationSystems_INFORMATIONSTORAGEANDRETRIEVAL ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Medicine ,GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries) - Abstract
Includes abstract. Includes bibliographical references.
- Published
- 2012
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