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Evidence has suggested the potential of tumor-educated platelets as a biomarker trove for cancer diagnostics, but the difficulty in isolation limits its application. Since most of the circulating RNAs are derived from platelets, the change of RNA profile in platelets may lead to altered RNA expression in serum. Here, we identified a panel of platelet-associated long non-coding RNAs (lncRNAs) and evaluated its diagnostic capacity in serum of colorectal cancer (CRC) patients. Four lncRNAs, LNCAROD, SNHG20, LINC00534, and TSPOAP-AS1, were upregulated in both platelets and serum of CRC patients. A binary logistic model derived from them has validated area under roc curve of 0.78 indicating great performance. Furthermore, the expression levels of LNCAROD and TSPOAP-AS1 were correlated with cancer staging and tumor location. Together, our results add novel lncRNA biomarkers to the list of blood tests for CRC diagnostics and provide molecular evidence for the cross-talk between CRC platelets and serum.

Organic single-component ferroelectrics, as an important class of metal-free ferroelectrics, are highly desirable because of their easy processing, mechanical flexibility, and biocompatibility. However, although nearly 50 years have passed since the discovery of photochromism in azobenzene-doped cholesteric liquid crystals, ferroelectricity has never been found in azobenzene-based crystals. Here, we use an amino group to substitute a fluorine atom of 2,2',4,4',6,6'-hexafluoroazobenzene, which successfully introduces ferroelectricity into 2-amino-2',4,4',6,6'-pentafluoroazobenzene (APFA). APFA shows an extremely high Curie temperature (

A new porous liquid system based on the coalition of MOPs and bulky IL was designed. The liquid materials exhibit permanent cavities, long-term durability, and good ability to separate CO2 from N2 and CH4 not only as an entity but also as a membrane.

As an essential element, magnesium is involved in a variety of physiological processes. Magnesium is the second most abundant cation in cells and the fourth most abundant cation in living organisms. Magnesium-based biomaterials are considered to be biocompatible and biodegradable, and they are promising for application in biomedicine. Among them, magnesium phosphates have attracted increasing interest in biomedical fields in recent years, because both magnesium and phosphorus are common elements in the human body. Magnesium phosphates exhibit many similar characteristics, such as excellent biocompatibility and pH-responsive degradability, to calcium phosphates that are widely applied in biomedicine. Magnesium phosphates are used in nanomedicine in the form of monodisperse particles, or used in tissue engineering in the form of cements, ceramics, scaffolds, coatings and so on. This review focuses on the state-of-the-art progress made in magnesium phosphate-based biomaterials and their biomedical applications, including nanostructured magnesium phosphates and magnesium phosphate-based cements, ceramics, scaffolds, coatings and so on. Finally, the challenges and future research directions of magnesium phosphate-based biomaterials are also explored.

We perform a thorough analysis of the $η-η'$ mixing effects on the $Λ_b\rightarrow Λη^{(')}$ decays based on the perturbative QCD (PQCD) factorization approach. Branching ratios, up-down and direct $CP$ asymmetries are computed by considering four popular mixing schemes, such as $η-η'$, $η-η'-η_c$, $η-η'-G$, and $η-η'-G-η_c$ mixing formalism, where $G$ represents the physical pseudoscalar gluball. The PQCD predictions with the four mixing schemes does not change much for the $η$ channel but changes significantly for the $η'$ one. In particular, the value of $\mathcal{B}(Λ_b\rightarrow Λη^{'})$ in the $η-η'-G-η_c$ mixing scheme exceeds the present experimental bound by a factor of 2, indicates the related mixing angles may be overestimated. Because of the distinctive patterns of interference between $S$-wave and $P$-wave amplitudes, the predicted up-down asymmetries for the two modes differ significantly. The obvious discrepancies among different theoretical analyses should be clarified in the future. The direct $CP$ violations are predicted to be at the level of a few percent mainly due to the tree contributions of the strange and nonstrange amplitudes suffer from the color suppression and CKM suppression. Finally, as a byproduct, we investigate the $Λ_b\rightarrow Λη_c$ process, which has a large branching ratio of order $10^{-4}$, promising to be measured by the LHCb experiment. Our findings are useful for constraining the mixing parameters, comprehending the $η^{(')}$ configurations, and instructing experimental measurements., 19 pages,1 figure,7 tables, updated to match published version

After UV illumination at 254 nm for 10 min, the incorporated LiYGeO4:Bi3+ in the PCN-PLM can continuously provide light irradiation at 365 nm to regulate the CO2 adsorption capacity of PCN-250.

Inflammatory reaction exerts a pivotal role in secondary damage after cerebral hemorrhage and spinal cord injury. miRNAs can both promote and inhibit inflammatory actions among microglial cells. The objective of the present paper was to figure out whether miR-27b-3p produced regulatory effects during processes of microglial inflammation. Lipopolysaccharides (LPS) were used to prepare microglial activation models. Following miR-27b-3p overexpression and interference, the RNA and protein levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β were subjected to real-time fluorescent quantitative PCR (qPCR) and western blot assays, respectively. Cellular apoptosis was subjected to flow cytometry and miR-27b-3p target genes were visualized using a dual luciferase reporter system for verification. The levels of TNF-α, IL-6, and IL-1β mRNA in miR-27b-3p-overexpressed microglial cells were markedly increased compared to the control. Apoptosis of microglial cells was increased markedly in the overexpressed miR-27b-3p group compared to the negative control. Conversely, a different result was presented in the microglial transfected with miR-27b-3p inhibitors. The downregulation of A20, a miR-27b-3p target gene, mediated levels of TNF-α, IL-6, and IL-1β. Furthermore, A20 reduced microglial apoptosis. These data revealed that miR-27b-3p could mediate not only microglia activation but also neuroinflammation via downregulating A20 expression. Thus, miR-27b-3p is regarded as gene therapy in treating cerebral hemorrhage and spinal cord injury., Graphical abstract

To investigate whether the outlet of the femoral tunnel will cause iatrogenic injury to the medial collateral ligament (MCL) during posterior cruciate ligament reconstruction (PCLR) and estimate the safe angle of femoral tunnel placement.Thirteen formaldehyde-soaked human knee joint specimens were used, 8 from men and 5 from women; the donors' age ranged from 49 to 71 years, with an average of 61 years. First, the medial part of the femur was carefully dissected to clearly expose the region of the MCL course and attachment on the femoral medial aspect and to outline the anterior margin of the region with a marked line. The marked line divided the medial femoral condyle into an area with an MCL course and a bare bone area which is regarded relatively safe for no MCL course. Then, the posterior cruciate ligament (PCL) was cut to identify the femoral attachment of the PCL. After the knee joint was fixed at a 120° flexion angle, the process of femoral tunnel preparation for the PCL single-bundle reconstruction was simulated. The inside-out technique was used to drill the femoral tunnel from the PCL femoral footprint inside the knee joint with an orientation to exit the medial condyle of the femur, and the combination angle of the two planes, the axial plane and the coronal plane, was adapted to the process of drilling femoral tunnels at different orientations. The following 15 angle combinations were used in the study: 0°/30°, 0°/45°, 0°/60°, 15°/30°, 15°/45°, 15°/60°, 30°/30°, 30°/45°, 30°/60°, 45°/30°, 45°/45°, 45°/60°, 60°/30°, 60°/45°, 60°/60° (axial/coronal). The positional relationship between the femoral tunnel outlet on the femoral medial condyle and the marked line was used to verify whether the tunnel drilling angle was a risk factor for MCL injury or not, and whether the shortest distance between the femoral exit center and the marked line was affected by the various angle combinations. Furthermore, the safe orientation of the femoral tunnel placement would estimated.When creating the femoral tunnel for PCLR, there was a risk of damage to the MCL caused by the tunnel outlet, and the incidence was from 0 to 100%; when the drilling angle of the axial plane was 0° and 15°, the incidence of MCL damage was from 69.23% to 100%. There was a significant difference in the incidence of MCL damage among femoral tunnels of 15 angle combinations (The creation of the femoral tunnel in PCLR can cause iatrogenic MCL injury, and the risk is affected by the tunnel angle. To reduce the risk of iatrogenic injury, angle combinations of 45°/45°, 45°/60°, 60°/30°, 60°/45°, and 60°/60° (axial/coronal) are recommended for preparing the femoral tunnel in PCLR.探讨后交叉韧带重建术（posterior cruciate ligament reconstruction，PCLR）中，股骨隧道在股骨内侧髁的开口是否会造成内侧副韧带（medial collateral ligament，MCL）医源性损伤，并估计股骨隧道放置的安全角度。.使用13具甲醛浸泡人体膝关节标本，捐赠者男8例，女5例；年龄49～71岁，平均61岁。仔细解剖每个膝关节标本股骨内侧区，明确MCL在股骨内侧走行与附着的区域，并沿此区域前缘作1条标记线，通过这条标记线将股骨内侧髁分割为MCL覆盖区和无MCL分布的相对安全区域。然后将膝关节标本的后交叉韧带（posterior cruciate ligament，PCL）切断以暴露PCL股骨附着区，在120° 屈曲角度固定的膝关节标本上，模拟PCL单束重建术中股骨隧道制备，采用由内向外技术从膝关节内部的PCL股骨足迹处向股骨内侧方向进行股骨隧道钻孔，并通过轴面和冠状面两平面角度组合的方式实现不同方向股骨隧道的创建。选取15种角度组合［0°/30°、0°/45°、0°/60°、15°/30°、15°/45°、15°/60°、30°/30°、30°/45°、30°/60°、45°/30°、45°/45°、45°/60°、60°/30°、60°/45°和60°/60°（轴面/冠状面）］，验证股骨隧道放置角度是否为损伤MCL的危险因素，并通过检验各角度组合产生的股骨隧道开口点与MCL前缘标记线的最短距离是否存在差异来估计股骨隧道放置的安全角度。.在PCLR中创建股骨隧道时，存在MCL被股骨隧道出口损伤的风险，发生率为0～100%，当轴平面钻孔角度为0° 和15° 时，MCL损伤发生率高达69.23%～100%。并且股骨隧道放置角度不同，其MCL损伤发生率差异有统计学意义（在PCLR中股骨隧道的创建会造成MCL医源性损伤，且损伤风险受隧道角度影响，推荐以45°/45°、45°/60°、60°/30°、60°/45° 和60°/60°（轴面/冠状面）角度组合创建股骨隧道，以降低MCL的医源性损伤风险。.

Grain growth can modify the microstructure of natural ice, including the grain size and crystallographic preferred orientation (CPO). To understand better grain-growth processes and kinetics, we compared microstructural data from synthetic and natural ice samples that were annealed at ice-solidus temperature (0 ºC) to successfully long durations. The synthetic ice has a homogeneous initial microstructure, which is characterised by polygonal grains, little intragranular distortion and bubble content, and a near-random CPO. The natural ice samples were sub-sampled from ice cores acquired from the Priestley Glacier, Antarctica; they have a heterogeneous microstructure, which is characterised by a considerable number of air bubbles, widespread intragranular distortion, and a preferred crystallographic alignment. During annealing, the average grain size of natural ice barely changes, whilst the average grain size of synthetic ice gradually increases. This observation suggests grain growth in natural ice can be much slower than synthetic ice; the grain-growth law derived from synthetic ice data cannot be directly applied to estimate the grain-size evolution in natural ice. The microstructure of natural ice characterised by many bubbles pinning at grain boundaries. Previous studies suggest bubble pinning reduces the driving force of grain boundary migration, and it should be directly linked to an inhibition of grain growth observed in natural ice. As annealing progresses, the number density (number per unit area) of bubbles on natural-ice grain boundaries decreases, whilst the number density of bubbles in grain interior increases. This observation indicates that some ice grain boundaries sweep through bubbles, which should weaken the bubble-pinning effect and thus enhance the driving force for grain boundary migration. Consequently, the grain growth in natural ice might comprise more than one stage and it should correspond to more than one set of grain-growth parameters. Some of the Priestley ice grains become abnormally large during annealing. We suggest the bubble-pinning, which inhibits the grain growth of ice matrix, and the contrast of dislocation-density amongst neighbouring grains, which favours the selected growth of individual grains with low dislocation densities, are tightly correlated with the abnormal grain growth.

Restoring the normal structure and function of injured tendons is one of the biggest challenges faced by the Department of Orthopedics and Sports Medicine. Tendon-derived stem cells (TDSCs), a new type of pluripotent stem cells with multidirectional differentiation potential, are expected to be promising cell seeds for the treatment of tendon injury and tendon-bone healing in the future. In this study, tendon stem cells were successfully isolated from human tissues, which were positive for markers CD44, CD90, and CD105, and exhibited clonality and multilineage differentiation ability. Analysis of single-cell sequencing results and mass spectrometry identification results showed that there were differences in protein expression during CTGF-induced TDSC tendon differentiation. Reverse Co-IP, qPCR, WB, and immunofluorescence detection all confirmed that CTGF directly interacts with KIT, thereby mediating the transcription factor HES1 to regulate the Wnt/β-catenin signaling pathway (GSK3β, β-catenin, TCF4). ChIP-qPCR and dual-luciferase reporter gene assays indicated that HES1 regulates stem cell differentiation by directly regulating the expression of GSK3β in the Wnt/β-catenin pathway. Rats were treated with TDSCs overexpressing the KIT gene after repair surgery. This method had a more ideal recovery effect than other methods through animal behavioral scores, mechanical properties testing, and HE staining tissue observation. This study found that the use of modified human tendon stem cells (hTDSC) could promote graft ligamentization and tendon-bone healing after ACL reconstruction, which could provide an effective way for faster and better recovery from tendon injury.

Methicillin-resistant Staphylococcus aureus (MRSA) is an uncommon but serious cause of community-acquired pneumonia (CAP). A lack of validated MRSA CAP risk factors can result in overuse of empirical broad-spectrum antibiotics. We sought to develop robust models predicting the risk of MRSA CAP using machine learning using a population-based sample of hospitalized patients with CAP admitted to either a tertiary academic center or a community teaching hospital. Data were evaluated using a machine learning approach. Cases were CAP patients with MRSA isolated from blood or respiratory cultures within 72 h of admission; controls did not have MRSA CAP. The Classification Tree Analysis algorithm was used for model development. Model predictions were evaluated in sensitivity analyses. A total of 21 of 1,823 patients (1.2%) developed MRSA within 72 h of admission. MRSA risk was higher among patients admitted to the intensive care unit (ICU) in the first 24 h who required mechanical ventilation than among ICU patients who did not require ventilatory support (odds ratio [OR], 8.3; 95% confidence interval [CI], 2.4 to 32). MRSA risk was lower among patients admitted to ward units than among those admitted to the ICU (OR, 0.21; 95% CI, 0.07 to 0.56) and lower among ICU patients without a history of antibiotic use in the last 90 days than among ICU patients with antibiotic use in the last 90 days (OR, 0.03; 95% CI, 0.002 to 0.59). The final machine learning model was highly accurate (receiver operating characteristic [ROC] area = 0.775) in training and jackknife validity analyses. We identified a relatively simple machine learning model that predicted MRSA risk in hospitalized patients with CAP within 72 h postadmission.

Rationale: Current guidelines recommend patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia receive empirical antibiotics for suspected bacterial superinfection on the basis of weak evidence. Rates of ventilator-associated pneumonia (VAP) in clinical trials of patients with SARS-CoV-2 pneumonia are unexpectedly low. Objectives: We conducted an observational single-center study to determine the prevalence and etiology of bacterial superinfection at the time of initial intubation and the incidence and etiology of subsequent bacterial VAP in patients with severe SARS-CoV-2 pneumonia. Methods: Bronchoscopic BAL fluid samples from all patients with SARS-CoV-2 pneumonia requiring mechanical ventilation were analyzed using quantitative cultures and a multiplex PCR panel. Actual antibiotic use was compared with guideline-recommended therapy. Measurements and Main Results: We analyzed 386 BAL samples from 179 patients with SARS-CoV-2 pneumonia requiring mechanical ventilation. Bacterial superinfection within 48 hours of intubation was detected in 21% of patients. Seventy-two patients (44.4%) developed at least one VAP episode (VAP incidence rate = 45.2/1,000 ventilator days); 15 (20.8%) initial VAPs were caused by difficult-to-treat pathogens. The clinical criteria did not distinguish between patients with or without bacterial superinfection. BAL-based management was associated with significantly reduced antibiotic use compared with guideline recommendations. Conclusions: In patients with SARS-CoV-2 pneumonia requiring mechanical ventilation, bacterial superinfection at the time of intubation occurs in

Increasing mining depths and intensities have resulted in frequent accidents during roadway excavation, thus increasing the necessity of preemptively determining the geological conditions of rock formations along roadways. Identifying the spatial distributions of lithotypes and the mechanical properties of non-marker bed intervals in coal measure strata are crucial for roadway horizon design, roof management, and efficient excavation. Therefore, based on petrophysical tests, well logging, and seismic data, a comprehensive method is proposed, which uses post-stack wave impedance inversion, petrophysical statistical analyses, and pre-stack simultaneous inversion to predict the lithology and elastic parameters of rocks surrounding roadways. The lithology of the target layer is first divided according to the wave impedances of the inversion body, and the sand lenses within the mudstone interval are described. Based on petrophysical test data, the shear-wave conversion equations of the sand lenses in this target interval and background mudstone are then obtained via marginal limit extrapolation. Finally, pre-stack simultaneous inversion, based on the seismic data and shear-wave conversion curves of different lithologies, is performed to obtain the elastic parameter profile of the target interval in the cross-section of the roadway, laying the foundation for further prediction of the mechanical properties of the surrounding rocks. The results agree with roadway drilling data by > 90%.

Cross-project defect prediction (CPDP) refers to identifying defect-prone software modules in one project (target) using historical data collected from other projects (source), which can help developers find bugs and prioritize their testing efforts. Recently, CPDP has attracted great research interest. However, the source and target data usually exist redundancy and nonlinearity characteristics. Besides, most CPDP methods do not exploit source label information to uncover the underlying knowledge for label propagation. These factors usually lead to unsatisfactory CPDP performance. To address the above limitations, we propose a landmark selection-based kernelized discriminant subspace alignment (LSKDSA) approach for CPDP. LSKDSA not only reduces the discrepancy of the data distributions between the source and target projects, but also characterizes the complex data structures and increases the probability of linear separability of the data. Moreover, LSKDSA encodes label information of the source data into domain adaptation learning process and makes itself with good discriminant ability. Extensive experiments on 13 public projects from three benchmark datasets demonstrate that LSKDSA performs better than a range of competing CPDP methods. The improvement is $ 3.44\%-11.23\%$ in g-measure , $ 5.75\%-11.76\%$ in AUC , and $ 9.34\%-33.63\%$ in MCC , respectively.

Many institutions suspended surveillance and contact precautions for multidrug-resistant organisms (MDROs) at the outset of the coronavirus disease 2019 (COVID-19) pandemic due to a lack of resources. Once our institution reinstated surveillance in September 2020, a vancomycin-resistant Enterococcus (VRE) faecium outbreak was detected in the cardiothoracic transplant units, a population in which we had not previously detected outbreaks.An outbreak investigation was conducted using pulsed-field gel electrophoresis for strain typing and electronic medical record review to determine the clinical characteristics of involved patients. The infection prevention (IP) team convened a multidisciplinary process improvement team comprised of IP, cardiothoracic transplant nursing and medical leadership, environmental services, and the microbiology laboratory.Between December 2020 and March 2021, the outbreak involved thirteen patients in the cardiothoracic transplant units, four index cases, and nine transmissions. Of the 13, seven (54%) were on the transplant service, including heart and lung transplant recipients, patients with ventricular assist devices, and a patient on extracorporeal membrane oxygenation as a bridge to lung transplantation. Four of 13 (31%) developed a clinical infection.Cardiothoracic surgery/transplant patients may have a similar risk for VRE-associated morbidity as abdominal solid organ transplant and stem cell transplant patients, highlighting the need for aggressive outbreak management when VRE transmission is detected. Our experience demonstrates an unintended consequence of discontinuing MDRO surveillance in this population and highlights a need for education, monitoring, and reinforcement of foundational infection prevention measures to ensure optimal outcomes.

Regulating the porosity of metal phosphonate frameworks is still challenging, even though this is not an issue for carboxylate-based metal-organic frameworks (MOFs). Quaternary ammonium cations are common template reagents widely used for structure control. However, it is not successful for uranyl phosphonate frameworks (UPFs) because the large volume sizes of templates make it challenging to enter the channels constructed by phosphonate ligands with small pore sizes and low dimensions. In this work, three new porous three-dimensional UPFs were synthesized using the phosphonate ligand and template reagents with the same geometry, namely, (TEA)

The nonleptonic two-body Λb→Λψ decays with ψ=J/ψ or ψ(2S) are investigated based on the perturbative QCD approach. These are color-suppressed processes in which the nonfactorizable contributions are confirmed to be dominant. Angular momentum conservation allows us to describe the concerned decays by four independent complex helicity amplitudes. It is observed that the negative-helicity states for the Λ baryon are preferred as expected in the left-handed nature of the charged-current interaction. The obtained results for the helicity amplitudes are used to compute the branching ratios and various observable parameters, which are then compared to the existing theoretical predictions and experimental data. In particular, we predict the ratio R=B(Λb→Λψ(2S))B(Λb→ΛJ/ψ)=0.47−0.00+0.02 in comparison with 0.508±0.023 from the Particle Data Group at the level of 1 standard deviation. We also briefly explore the long-distance contributions to the semileptonic Λb→Λl+l− decays in the kinematic regions where the dilepton invariant masses are around the J/ψ and ψ(2S) resonances.

OBJECTIVE To investigate the expression of miR-22-3p in breast cancer and the mechanism of targeting PLAGL2 to inhibit the invasion and migration in human breast cancer. STUDY DESIGN An experimental study. PLACE AND DURATION OF STUDY Department of Oncology and Department of General Surgery, The People's Hospital of China Three Gorges University, China, from March 2019 to December 2020. METHODOLOGY The miR-22-3p expression level in 41 paired human primary breast invasive ductal carcinoma tissues and para-cancer tissues was obtained by real-time fluorescence quantitative reverse transcriptase PCR (qRT-PCR). The effect of miR-22-3p on the proliferation of breast cancer cells was detected by growth curve method. Online software TargetScan was used to predict the target genes of miR-22-3p. The prediction results were verified by luciferase reporter gene assay and qRT⁃PCR. RESULTS MiR-22-3p expression was significantly decreased in the breast cancer tissues than in para⁃carcinoma normal breast tissues (p

Payenapara lleloneura Kurz. (Kan-zaw), an endemic medicinal plant only found in Tanintharyi Region of Myanmar, is widely used in the treatment of various cancer and different ailments. In the present research, the seeds were phytochemical investigated for their nutritional potential for their use as functional foods or novel diet oil resources. Nutritional evaluation showed that the seeds are rich in fats and carbohydrates (soluble sugars and starch). Fatty acid analyses showed that the seeds accumulate very rich α-eleostearic acid (α-ESA, 18:3Δ9cis,11trans,13trans), an important conjugated fatty acid, up to more than 70 ​% of total fatty acids. The seed oil derived from the Kan-zaw tree contains approximately 3.25 ​% β-eleostearic acid (18:3Δ9trans,11trans,13trans), an unusual conjugated fatty acid that imparts a potent anticancer application and industrially important drying qualities to Kan-zaw oil. Physicochemical properties of the Kan-zaw seeds were examined; petroleum ether (60–90 ​°C) extract of seed oils were also investigated for the saponification value, iodine value and estimation of acid value. Further, the present study investigated cytotoxic potential of ethanol, methanol, acetone, chloroform Kan-zaw seed extracts and commercial Kan-zaw oil against human cervical cancer cell line (HeLa). The Kan-zaw extracts and oil have shown significant anticancer activity on HeLa cells.

Metal–organic polyhedra (MOPs) have emerged as versatile platforms for artificial models of biological systems due to their discrete structure and modular nature. However, the design and fabricatio...

Retraction of ‘Sericin hydrogels promote skin wound healing with effective regeneration of hair follicles and sebaceous glands after complete loss of epidermis and dermis’ by Chao Qi et al., Biomater. Sci., 2018, 6, 2859–2870, https://doi.org/10.1039/C8BM00934A.