Objective: To investigate the clinical significance of detection of blood lipids and estrogen levels in patients with pregnancy combined with hepatitis B. Methods: From August 2014 to April 2017, 80 patients with pregnancy combined with hepatitis in our hospital were selected as the observation group. Meanwhile, 80 pregnant women without hepatitis B were selected as the control group. The clinical data of two groups were investigated and collected. The serum HDL-C, TC, TG, HDL-C and estrogen levels were compared between the two groups. The blood lipids and serum estrogen levels of different pregnancy outcomes in two groups were compared. Logistic multiple regression analysis was performed in the observation group, using adverse pregnancy outcome as the dependent variables and the levels of blood lipids and estrogen as independent variables, to analyze the risk factors of pregnancy adverse outcome in pregnant women with hepatitis B. Results: Compared with control group, the serum levels of LDL-C, TC and TG in the observation group were higher than those in the control group, while the serum level of HDL-C in the observation group was significantly lower than that in the control group (P〈0.05), and the difference was statistically significant. The levels of serum estrogen in the observation group (154.20± 10.82 pmol/L) was significantly higher than that in the control group (88.14± 8.98 pmol/L, t=8.109, P〈0. 05). Compared with control group, the occurrence of cesarean section, postpartum hemorrhage, neonatal asphyxia, fetal distress, pregnancy-induced hypertension and so on in the observation group were significantly higher than that in the control group (P〈0.05). The levels of serum TC, TG and LDL-C in the observation group and the control group were significantly higher than those in the normal outcome group, while the HDL-C content was significantly lower than that in the normal pregnancy group. The LDL-C, estrogen, age, duration of hepatitis B were the risk factors that lead to adverse pregnancy outcome in pregnancy with hepatitisB (P<0.05). Conclusion: The incidence of adverse pregnancy outcome in patients with hepatitis B is high, and it is accompanied by dyslipidemia and high-expression of estrogen which can be used as predictors of pregnancy combined with hepatitis B prognosis [ABSTRACT FROM AUTHOR]