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Stem canker and black scurf caused by Rhizoctonia solani are the important diseases in potato, while spur blight caused by Didymella applanata is a major disease in red raspberry. In Western Siberia, both crops are grown predominantly in small-scale farming that requires maximal usage of biological products for plant protection instead of chemicals. We evaluated two promising Bacillus velezensis strains BZR 336 g and BZR 517 isolated in the south of Russia (45°1'N, 38°59'E) for their biological control potentials against the potato and red raspberry diseases under the more severe weather conditions of Western Siberia (55°1'N, 82°55' E). We tested two techniques to apply biocontrol agents: (1) coating the seeds (potato tubers) and (2) spraying over the plants (raspberry canes). In each case, we estimated B. velezensis strains on two plant cultivars differed by the disease resistance. The degree of B. velezensis influence on disease incidence and severity depended on the bacterial strain, the protected plant, and its cultivar. We also demonstrated that two B. velezensis strains significantly stimulated plant growth of potato, which contributed to the plant productivity on both cultivars. The BZR 336 g strain affected the potato productivity more than the BZR 517 strain. Under the influence of both bacterial strains, raspberry yield was significantly higher compared to the control on the susceptible cultivar. These findings indicated that two southern B. velezensis strains had proved their efficacy as biological control agents in the control of the serious fungal infection of potato and raspberry plants under the more severe ecological conditions of Western Siberia. For the first time, we demonstrated B. velezensis strains potential for use as biological control agents against R. solani on potato, and against D. applanata on red raspberry. [ABSTRACT FROM AUTHOR]

Seeking a means of sanitizing berries, the effectiveness of steady state levels of gaseous chlorine dioxide (ClO2) against hepatitis A virus (HAV) on laboratory-contaminated berries was determined. The generated ClO2 was maintained with 1 or 2 mg/l air inside a 269-l glove box to treat 50 g batches of blueberries, raspberries, and blackberries, and 100 g batches of strawberries that were immersion coated with HAV. Normalized data for ClO2 (ppm-h/g product) is reported as a function of ClO2 concentration, treatment time, and weight of treated product. Treatments of ClO2 ranging from 1.00 to 6.27 ppm-h/g berry were evaluated. When compared to untreated HAV-contaminated berries, log reductions of HAV were > 2.1 for all berry types and conditions tested indicating the gaseous ClO2 was effective. The average log reduction with strawberries, raspberries, blueberries and blackberries treated with 1.00 ppm-h/g, the lowest ClO2 treatment tested, were 2.44, 2.49, 3.23, and 3.45, respectively. The highest treatment of 6.27 ppm-h/g was applied at two different gas concentrations of 1 mg/l and 2 mg/l. Average log reductions for blueberries and strawberries treated with 6.27 ppm-h/g were 4.34 and 4.42, and 4.03 and 3.51, applied at 1 mg/l and 2 mg/l, respectively. For blackberries and raspberries 3.20 and 3.24, and 3.23 and 3.97 log reductions were observed for 6.27 ppm-h/g treatments applied at 1 mg/l and 2 mg/l, respectively. Results indicate that HAV contamination of berries can be substantially reduced by gaseous ClO2 and offer industry a waterless means of sanitizing berries against HAV. [ABSTRACT FROM AUTHOR]

In this study, we carried out an exhaustive characterization of the polyphenol profile of strawberry, raspberry, blueberry, and blackberry decoctions; as well as the extractable (EPP) and hydrolysable (HPP) polyphenolic and proanthocyanidin profile of the residues obtained during berries decoctions (decoction by-products) by UPLC-ESI-Q-ToF MS. A greater variety of polyphenols was identified in the EPP fraction of the berry decoction by-products as compared to the decoctions, which were rich in several ellagitannins, flavonols, hydroxybenzoic acids, and anthocyanins. Ellagic acid was identified as the major polyphenol after acid hydrolysis in all berry decoction by-products, followed by hydroxybenzoic acid, which was found bound to the matrix by glycosidic, ether and ester bonds. Regarding proanthocyanidins, procyanidin, prodelphinidin, and propelargonidin dimers were identified in the strawberry, raspberry, and blackberry decoction by-products by MALDI-ToF MS. The results obtained from this study demonstrates that berries decoctions are rich in polyphenols; however, berry decoction by-products were richer in polyphenols with a great variety of extractable and non-extractable polyphenols. [ABSTRACT FROM AUTHOR]

Obesity and associated liver diseases are becoming global public health challenges. Raspberry (Rubus chingii Hu.), as a medicine food homology plant, possesses a series of health-promoting properties, but its protective effect on obesity-related liver injury and the potential mechanisms remain obscure. Herein high-fat diet (HFD)-fed mice were orally treated with raspberry polysaccharides (RCP) for 14 weeks. Treatment with RCP alleviated obesity and associated symptoms including hyperglycemia, hyperlipemia, endotoxemia, as well as hepatic inflammation and oxidant stress in HFD-induced obese mice. RCP restructured the gut microbiota and host metabolism especially by increasing the levels of Dubosiella and its metabolite butyrate. Besides, exogenous butyrate supplementation protected against intestinal barrier disruption, and thereby reduced inflow of lipopolysaccharide and mitigated inflammation and oxidative injury in the liver of obese mice. Therefore, we suggest that RCP can be utilized as a novel prebiotics to improve obesity-induced hepatic oxidative injury by enhancing butyrate-mediated intestinal barrier function. • Raspberry polysaccharides (RCP) reduce hepatic oxidative stress in obese mice. • RCP increases levels of butyrate-producing Dubosiella and its metabolite butyrate. • RCP enhances butyrate-mediated intestinal barrier function in obese mice. • RCP improves obesity-induced liver injury by enteral therapy. [ABSTRACT FROM AUTHOR]

[Display omitted] • Anthocyanins-centered voltammetry of lyophilized blueberry and raspberry fruits in oxygen-rich solution differs from that in the absence of oxygen. • Under ordinary electrochemical conditions, anthocyanidins and anthocyanins oxidation involved o -catechol units in the B-ring. • Under ROS-generating conditions, anthocyanin oxidation was associated with the loss of sugar units. • The reaction of anthocyanins with ROS results in species with very low oxidation potential. An electrochemical study of lyophilized blueberry (Vaccinium corymbosum L.) and raspberry (Rubus idaeus L.) fruits was carried out using microparticulate deposits from ethanolic extracts of fruits in contact with different aqueous electrolytes. This voltammetry is dominated by the oxidation of anthocyanins. It was hypothesized that under conditions of electrochemical generation of reactive oxygen species (ROS), new anthocyanin-derived species with high antioxidant capacity are formed over a wide pH range. In the case of ROS-activated anthocyanins, electrochemical oxidation is associated with loss of sugar moieties. Experimental voltammetric and scanning electrochemical microscopy (SECM) imaging data consistent with this hypothesis are presented. [ABSTRACT FROM AUTHOR]

Rubus yellow net virus (RYNV) belongs to genus Badnavirus. Badnaviruses are found in plants as endogenous, inactive or activatable sequences, and/or in episomal (infectious and active) forms. To assess the state of RYNV in Rubus germplasm, we sequenced the genomes of various cultivars and mined eight raspberry whole genome datasets. Bioinformatics analysis revealed the presence of a diverse array of endogenous RYNV (endoRYNV) sequences that differ significantly in their structure; some lineages have nearly complete, yet non-functional genomes whereas others have rudimentary, short sequence fragments. We developed assays to genotype the main lineages as well as the only known episomal lineage present in the United States. This study discloses the widespread presence of endoRYNVs in commercial raspberries, likely because breeding efforts have focused on a limited pool of germplasm that harbored endoRYNVs. • Rubus yellow net virus (RYNV) endogenous genomes (endoRYNV) differ significantly in their structure. • There are seven major endoRYNVs lineages identified in raspberry. • All integration events occurred before or during 19th century. • EndoRYNVs are widespread in commercial raspberries. • No endoRYNVs were identified in blackberry. [ABSTRACT FROM AUTHOR]

When choosing land for raising a new planting of vegetables or fruit crops, the composition of the soil plays an important role; therefore, the composition of soil is examined, and chemical analysis of soil is made. The culture that we examine is a raspberry planting. Raspberry is amulti-annual plant in the form of a bush or semi-shrub,with more annual roots and with one-year or two-year-old offshoots belonging to the Rosaceae family. Raspberries are irrigated by a drop-by-drop system, because through that system, remediation of soil can be carried out by adding appropriate crystalline fertilizers. Nitrogen (N) is the element with the greatest movement in the soil itself and its content is from 0.05 - 0.14 %. The pH value affects the calcium content in soil. Calcium plays an important role in the hardness of fruit and the sensitivity of physiological disturbance amounts to 1-2.1 %. Phosphorus is important for the fruit properties during storage and amounts to 14.7 - 40 kg / da. Potassium is a major element and plays a major role in producing good yields in fruit trees and amounts to about 14-50 kg / da. When mentioning magnesium, its role is not the most important and amounts to 74.5-221 ppm. The main role of magnesium lies within the ratio of potassium (K / Mg), which should range from 2: 1 to 3: 1. With the obtained soil analysis data, in addition to the climatic conditions, type and structure,which are equally important for raising a raspberry plant, we have a full understanding of the soil processes and guidelines for suitable conditions for growing raspberries.

Background and Aim: A variety of bacteria like Staphylococcus aureus utilize quorum sensing to perform their important activities such as biofilm formation and production of virulence factors. Interfering with the bacterial QS will disable the bacteria to perform above-mentioned vital activities. The principal aim of the study was to evaluate the effects of five plant extracts against bacterial Quorum sensing of Staphylococcus aureus. Materials and Methods: Thirteen strains of Staphylococcus aureus were isolated from patients with dental implant infection and identified. The plant species were collected from vicinity gardens of Fars Province and extracted using 96% ethanol. The anti-QS and antimicrobial susceptibility methods were then carried out to evaluate their bactericidal and QS properties with the use of Agrobacterium tumefaciens NTL/PZLR4. Furthermore, the biofilm production of the isolates was evaluated by microtiter plate (MTP) assay. Ethical Considerations: This study with research ethics codes 01-16-1-1959 and 73/118248 has been approved by research ethics committee at Islamic Azad University, Jahrom and research sciences branch, respectively. Findings: The results of the study disclosed that the extract of raspberry blossom possesses significantly (p21 mm). The anti-QS activity was proved by creating clear halo sides of the wells formed by Agrobacterium tumefaciens NTL/PZLR4. Moreover, the extracts of tarragon, wheat flower, flixweed and basil showed antimicrobial properties. Conclusion: According to the anti-biofilm and anti-QS properties of raspberry blossom extract against the isolates of Staphylococcus aureus, it could be considered as a mouthwash against dental bacterial infection with the identification of active compounds in the raspberry.

Intelligent surveillance systems are widely used in several critical areas as airports, ATM, and bank agencies to ensure more security and more safety. The need for an intelligent behavior recognition system is still increasing. Traditional approaches based on access to restricted places or suspected actions as theft, scam, and loitering are insufficient to identify suspect behavior. These actions do not represent a real key of suspects. Novel trends attempt to extract behaviors from involuntary actions as face gestures, face characteristics, and feeling features. This paper is motivated not only by the limits of the traditional approaches but also by the complexity of intelligent algorithms. In this context, the present paper uses face features to recognize the feeling of fear like a suspect behavior. Indeed, this feeling represents the main characteristic of a suspicious person under crime as announced by several psychologist scientists. The fear feeling is usually followed by an increase in heart rate beats. This paper describes the recognition of fear feeling using a camera as a contactless sensor. Frequencies associated with face based-video are used to estimate the heart rate according to the fusion of three techniques: bandpass filter, Eulerian transformer, and Lagrangian transformer. The proposed algorithm benefits from the advantages of each technique, but it is challenged by the Real-Time exigence. For this purpose, a Raspberry PI3 board is used in relation to Raspbian Operating System to ensures Real-Time criteria. The proposed is trained according to CK+ dataset. In this paper, contributions attempt to ensure not only a high recognition rate using a non-complex algorithm but also guarantee a real-time computation. Results reveal that the proposed algorithm has the best heart rate estimation in comparison with traditional methods. Hardware results justify the success of the proposed design in terms of resource requirements.

Drosophila suzukii (Matsumura, 1931) is an invasive oligophagous pest native to southeastern Asia. The first documented report of this pest in Serbia was in 2014. The insect is a pest of healthy and physiologically mature (ripening) soft fruits. The aim of the study was to determine the efficiency of different types of baited traps in mass trapping specimens of this species in raspberry plantings. Five types of blends (attractants) were used for mass trapping. The experiment was set up on May 27th, 2017. Inspection and sample collections were carried out at 7-10 day intervals from June 4th to July 31st, 2017. Based on the analysed field data, we confirmed that standard trap S was the most efficient, with 8099 specimens caught in the experimental period. The number of caught specimens decreased in the deployed traps, as follows: trap T4 (695), trap T3 (682), trap T2 (643), and trap T1 (482) as the least efficient. Statistical analyses showed very significant differences between the standard trap and trap T1, and significant differences between the standard trap and the other trap types in the average number of caught specimens. No significant differences in trapping were found between the other trap types.

The aim of this study was to investigate the effect of blackberry and raspberry fruits (1 and 4%) and leaves (0.5 and 1%) on the biological activities of rape honey. Honey and plant material extracts were analyzed regarding total phenolic, flavonoid, anthocyanin contents, HPTLC and HPLC polyphenol profiles, as well as antioxidant activity. The antiviral potential was analyzed against bacteriophage phi 6—a coronavirus surrogate—whereas antimicrobial was tested against S. aureus and E. coli. Blackberry extracts were more abundant in antioxidants than raspberry extracts, with better properties found for leaves than fruits and for cultivated rather than commercial plants. The addition of both Rubus plant additives significantly increased the antioxidant potential of honey by four-fold (for 4% fruits additive) to five-fold (for 1% of leaves). Honey with the addition of fruits possessed higher antiviral potential compared with raw rape honey (the highest for 4% of raspberry fruit and 1% of blackberry leaf additive). Honey enriched with Rubus materials showed higher antibacterial potential against S. aureus than rape honey and effectively inhibited S. aureus biofilm formation. To summarize, honey enriched with Rubus fruit or leaves are characterized by increased pro-health value and can be recommended as a novel functional food.

The increasing commercial production of R. idaeus offers insects nectar and pollen rewards, thus increasing the chance of cross-pollination, which enhances fruit yields. The knowledge of nectar characteristics may help farmers/beekeepers to improve the quality of their products. Therefore, we determined and compared nectar weight, sugar concentration and weight, and the qualitative and quantitative composition of sugars and amino acids in the nectar of six raspberry cultivars: three biennial and three repeated fruiting cultivars. The nectary abundance in these cultivars ranged between 20.2 ± 3.84 mg (‘Polka’) and 26.4 ± 7.3 mg (‘Glen Ample’) of nectar per flower with a sugar concentration of 34.6 ± 5.61–47.3 ± 9.33%. The contents of glucose and fructose (g/100 g) were in the range from 42.96 ± 0.71 (‘Glen Ample’) to 46.94 ± 0.55 (‘Laszka’) and from 50.7 ± 1.43 (‘Polka’) to 54.2 ± 0.72 (‘Radziejowa’). Sucrose was detected only in ‘Glen Ample’ (5.6 ± 1.12 g/100 g) and ‘Polka’ (6.2 ± 0.95 g/100 g). Taking into account the increasing sugar weight in the nectar, the cultivars were ranked as follows: ‘Polka’ < ‘Polana’ < ‘Radziejowa’ < ‘Pokusa’ < ‘Laszka’ < ‘Glen Ample’. The nectar of the analyzed raspberry cultivars was classified as a hexose-dominant type. Aspartic acid, glutamic acid, and proline were the most dominant endogenous amino acids, whereas exogenous acids were dominated by lysine and leucine. The present results provide valuable information about the nutritious value of R. idaeus nectar for pollinators.

The main objective of this study was to extend the shelf life of fresh red raspberry (Rubus idaeus. L. cv. ‘Kweli’) by using active film-pads inside commercial compostable packages. The pads were produced with chitosan (Ch) with the incorporation of green tea (GTE) and rosemary (RSME) ethanolic extracts as natural antifungal agents. Pads were placed on the bottom of commercial fruit trays underneath the fruits, and the trays were heat-sealed with a polyacid lactic (PLA) film. Preservation studies were carried out over 14 days of storage at refrigeration temperature (4 °C). Raspberry samples were periodically analyzed throughout storage, in terms of quality attributes (fungal decay, weight loss, firmness, surface color, pH, total soluble solids), total phenolic content and antioxidant activity. Gas composition inside the packages was also analyzed over time. From the packaging systems tested, the ones with active film-pads Ch + GTE and Ch + RSME were highly effective in reducing fungal growth and decay of raspberry during storage, showing only around 13% and 5% of spoiled fruits after 14 days, respectively, in contrast with the packages without pads (around 80% of spoiled fruits detected). In addition, fruits preserved using packages with Ch + RSME active film-pads showed lower mass loss (5.6%), decreased firmness (3.7%) and reduced antioxidant activity (around 9% and 15% for DPPH and FRAP methods, respectively). This sustainable packaging presents a potential strategy for the preservation of raspberries and other highly perishable small fruits.

Raspberries (Rubus idaeus) possess a wide phenolic family profile; this serves the role of self-protection for the plant. Interest in these compounds have significantly increased, since they have been classified as nutraceuticals due to the positive health effects provided to consumers. Extensive chemical, in vitro and in vivo studies have been performed to prove and validate these benefits and their possible applications as an aid when treating several chronic degenerative diseases, characterized by oxidative stress and an inflammatory response. While many diseases could be co-adjuvanted by the intake of these phenolic compounds, this review will mainly discuss their effects on cancer. Anthocyanins and ellagitannins are known to provide a major antioxidant capacity in raspberries. The aim of this review is to summarize the current knowledge concerning the phenolic compound family of raspberries, and topics discussed include their characterization, biosynthesis, bioavailability, cytotoxicity, antioxidant and anti-inflammatory activities.

Стаття присвячена проблемі використання апаратних засобів при навчанні студентів розподіленому програмуванню. Установлено, що комплексне використання програмно-апаратних засобів при вивченні розподіленого програмування є одним з важливих методів для розвитку професійних компетентностей інженерів-програмістів. Виявлено, що для ефективного навчання студентів розподіленому програмуванню необхідна велика кількість ресурсів для розробки цих обчислень, які є достатньо компактними, щоб вписатися в рамки одного курсу та достатньо автономними. Одним з методів вивчення розподілених обчислень є використання навчального обчислювального кластеру. Розробка навчального обчислювального кластеру містить такі основні етапи: розробка навчальних завдань, для виконання яких потрібно використання обчислювального кластеру; розробка апаратної частини; встановлення операційної системи; проєктування мережі передавання даних між вузлами кластеру; налаштування головного вузла та програм керування кластером; встановлення програмного забезпечення для розробки розподілених обчислень. Тому використання кластеру допоможе розвитку професійних компетентностей майбутніх інженерів-програмістів при вивченні дисципліни «Паралельні та розподілені обчислення», яка викладається при професійній підготовці інженерів-програмістів зі спеціальності «Комп’ютерні науки». Для використання в якості вузлів обчислювального кластеру було взято для порівняння персональні комп’ютери та одноплатні комп’ютери Raspberry. Після порівняльного аналізу було проведено моделювання навчального обчислювального кластеру засобами одноплатних комп’ютерів Raspberry. У результаті тестування було виявлено, що на великих об’ємах даних, для математичних обчислень, навчальний обчислювальний кластер на одноплатних комп’ютерах Raspberry робить обчислення в півтори рази ефективніше, ніж персональний комп’ютер. Проведений аналіз розвитку професійних компетентностей при моделювання, розробці та використанні навчального обчислювального кластеру.

The spotted wing drosophila (SWD) causes massive losses in red raspberry (Rubus idaeus L.) cultivation by direct oviposition leading to damages of the soft skin fruits. Knowledge of the fly's host preference could help farmers in managing the pest. We used a laboratory‐based oviposition assay for screening the germplasm of Rubus to ascertain whether the spotted wing drosophila prefers certain cultivars to others for oviposition and if preference is based on citric acid and Brix content or firmness. Correlation analyses of evaluated characters with no‐choice tests results in 3 years obtained no correlation between citric acid, Brix content and oviposition. Primocane raspberries were lower affected by SWD than floricane raspberries. The Rubus hybrid cultivar "Dorman Red" and the primocane cultivar "Pokusa" showed the lowest oviposition rate compared to the other 58 evaluated genotypes. We found that oviposition correlates to firmness of the investigated cultivars, which strongly indicates that host preference is partly connected to that character in raspberries. The results are discussed regarding the use of Rubus genetic resources in breeding and integrated pest management to control spotted wing drosophila in the field. [ABSTRACT FROM AUTHOR]

Spatial aspects of connectivity have received considerable attention from ecologists and conservationists, yet temporal connectivity, the periodic linking of habitats, plays an equally important, but largely overlooked role. Different biological and biophysical attributes of ecosystems underpin temporal connectivity, but here we focus on resource continuity, the uninterrupted availability of foraging sites. We test the response of pollinators to resource continuity at community, population, and individual levels using a novel natural experiment consisting of farms with either single or sequential cropping systems. We found significant effects at the population level; colony density of an important crop pollinator (Bombus impatiens L.) was greater when crop floral resources were continuously available. However, we did not find significant effects at the community or individual level; wild bee abundance, diversity and body size did not respond to resource continuity. Raspberry farms with greater early season resources provided by blueberry had greater bumble bee populations, suggesting beneficial effects on resource availability due to crop diversity. Better understanding the impact of resource continuity via crop diversity on broader patterns of biodiversity is essential for the co‐management of biodiversity and ecosystem services. [ABSTRACT FROM AUTHOR]

Due to the increasing microbial tolerance to commonly used food preservatives, as well as growing consumer awareness of their adverse impact on human health, alternative methods of pathogens reduction in food are widely investigated. The aim of this research was to examine the antimicrobial activity of red raspberry pomace and seed preparations against enterohemorrhagic Escherichia coli (EHEC), Salmonella Typhimurium, Salmonella Enteritidis, Listeria monocytogenes, and probiotic Lactobacillus rhamnosus strain. The combined action of LAB (lactic acid bacteria) and raspberry preparations on the pathogenic species was also evaluated. The results of our study showed no or weak antibacterial effect of raspberry preparations on the pathogenic bacteria tested. Regardless of preparation concentration (1.0 or 2.0 mg/mL), the bacteria number after 48-h incubation was usually higher than in the culture at the initial stage and varied from 105 to 107 cfu/mL. On the other hand, probiotic Lactobacillus rhamnosus strain caused a significant reduction in the enteric pathogen count after 24-h co-culture with LAB. The concentrations of both Salmonella serotypes were below the detection limit of the analytical methods applied. Moreover, the combined use of LAB and raspberry preparations resulted in the total elimination of Salmonella strains and the reduction in L. monocytogenes number from 105 to 102-104 cfu/mL after 24-h co-culture. EHEC revealed the highest resistance to the mixed culture effect. The synergic antimicrobial effect suggests the possibility of applying probiotic bacteria and berry preparations as natural antimicrobial agents in the food industry. [ABSTRACT FROM AUTHOR]

The major aim of this research was to investigate the effect of ozone treatment on the energy metabolism in raspberry fruit during storage at room temperature. Raspberries were ozonated with an ozone concentration of 8–10 mg L−1, for 30 min, every 12 h of storage at room temperature for 72 h. The results indicated that ozone treatment significantly enhanced the activities of mitochondrial respiratory enzymes, such as succinate dehydrogenase, cytochrome C oxidase, and H+-ATPase, which contributed to maintain the high level of ATP and energy charge in fruit during storage. Moreover, the energy metabolism in mitochondria was closely correlated with the antioxidant potential of raspberry fruit. This study has given an experimental evidence that ozonation procedure in proposed process conditions significantly affects the mitochondrial respiratory system leading to maintain the high quality of the fruit over a long period of storage at room temperature. [ABSTRACT FROM AUTHOR]

Hypertension is one of the main risk factors for cardiovascular disease and causes widespread morbidity and mortality worldwide. Although several antihypertensive drugs have been proposed for management of high blood pressure, changing lifestyle, including diet, has attracted interest recently. In this sense, consumption of fruits and vegetables, which are rich in vitamins, minerals, and phytochemicals, has been assigned as an efficient therapeutics. Berry fruits contain various bioactive compounds with potential health implications such as antioxidant, antimicrobial, anticancer, and anti-inflammatory properties. The main mechanisms responsible for antihypertensive activity mainly arise from the activity of flavonoids, minerals, and vitamins, as well as fibers. The objective of this review is to provide a summary of studies regarding the effect of berry fruits on the hypertensive animals and humans. The mechanisms involved in reducing blood pressure by each group of compounds have been highlighted. It can be concluded that berries' bioactive compounds are efficient in mitigation of hypertension through improvement of vascular function, angiotensin-converting enzyme's (ACE) inhibitory activity, increasing endothelial nitric oxide synthase (eNOS) activity, and nitric oxide (NO) production, besides anti-oxidative and anti-inflammatory activities. These fruits can be considered as potential sources of invaluable compounds for development of antihypertensive foods and pharmaceuticals. [ABSTRACT FROM AUTHOR]

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Blackberry and raspberry have high nutritional, health value, and are popular with consumers for their unique flavors. To explore the relationships between nutrient accumulation, antioxidant substance contents in blackberry and raspberry fruits, and fruit growth and development, seven Rubus cultivars were selected, and contents of the main active substance were determined. “Clode Summit” had the highest soluble sugar and fructose contents, “Chester”—the highest total phenol content, and “Bristol’—the highest anthocyanin content. Generally, the contents of flavonoids and total phenols showed a downward trend with the development of fruit in seven Rubus cultivars, and the content of anthocyanins increased rapidly in the later stage of development. Pearson correlation analysis showed extremely significant correlation between antioxidant activity and the contents of vitamin E, total phenols, and flavonoids. Flavonoids were extremely significantly positively correlated with the content of total phenols, and the contents of flavonoids and anthocyanins in various cultivars were highly negatively correlated. Considering the different nutritional ingredients and active antioxidant substance contents, “Clode Summit”, “Bristol”, and “Chester” are recommended for raw consumption, processing, and medicinal purposes, respectively. These results provide a reference for comparing the main active substance contents in different Rubus cultivars and their changes across fruit development stages.

The aim of this exploratory study was to evaluate the gut microbial signatures of distinct trimethylamine N-oxide (TMAO) responses following raspberry consumption. Investigations were carried out in 24 subjects at risk of developing metabolic syndrome who received 280 g/day of frozen raspberries for 8 weeks. Blood and stool samples were collected at weeks 0 and 8. Inter-individual variability in plasma TMAO levels was analyzed, 7 subjects were excluded due to noninformative signals and 17 subjects were kept for analysis and further stratified according to their TMAO response. Whole-metagenome shotgun sequencing analysis was used to determine the impact of raspberry consumption on gut microbial composition. Before the intervention, the relative abundance of Actinobacteriota was significantly higher in participants whose TMAO levels increased after the intervention (p = 0.03). The delta TMAO (absolute differences of baseline and week 8 levels) was positively associated with the abundance of gut bacteria such as Bilophila wadsworthia (p = 0.02; r2 = 0.37), from the genus Granulicatella (p = 0.03; r2 = 0.48) or the Erysipelotrichia class (p = 0.03; r2 = 0.45). Changes in the gut microbial ecology induced by raspberry consumption over an 8-week period presumably impacted quaternary amines-utilizing activity and thus plasma TMAO levels.

Drosophila suzukii (spotted wing drosophila—SWD) is an economically important pest of soft and stone fruit worldwide. Control relies on broad-spectrum insecticides, which are neither fully effective nor environmentally sustainable. The sterile insect technique (SIT) is a proven, effective and environmentally friendly pest-management tool. Here, we investigated, for the first time, the potential of using SIT to control D. suzukii in field conditions without physical barriers that limit insect invasion. A proprietary method of rearing and irradiation with X-rays was used to obtain males that were > 99% sterile. Sterile males were released twice per week from April to October 2021 on a site in Kent, UK, where everbearing strawberries were grown in open polytunnels. The infestation of wild female D. suzukii was monitored weekly using red sticky traps with dry lure at the treated site and at two similar control sites that did not receive sterile male releases. Releases of sterile males suppressed the wild female D. suzukii population by up to 91% in comparison with the control sites. We thus demonstrated the feasibility of SIT to achieve season-long control of D. suzukii using early, sustained and dynamically targeted releases of sterile males. This provides a promising environmentally friendly method to control this important pest.

Functional food is lately an interesting topic from the new product development perspective; complex motivation and expectations of consumers regarding it present a challenge when new products are designed. Co-creation is an interesting alternative to the standard practices by the R & D departments since it directly involves consumers in the various stages of the creation process. This work aims to describe experiences of engaging consumers in different development stages of a functional food product within a project realized at a food research institute. Four consecutive studies were conducted: the first study explored current trends in Serbia regarding the way consumers use functional food and are informed about it; the second study described development of a raspberry seeds extract with antioxidant and anti-proliferative activity confirmed in vitro; the third study tested the same extract in a sample of consumers, validating its usability in food products; and the fourth study described a co-creation session with 18 participants, during which a number of activities were realized to stimulate idea generation. Rather than the final product idea itself, this work is valuable because of detailed insights into the various phases of the co-creation process. It is shown that consumers and food researchers can together engage in the new food product development process as long as the communication between them is rich and with mutual understanding.

Industrial processing of raspberry juice and wine generates considerable byproducts of raspberry pomace. Ellagic acids/ellagitannins, being characterized by their antioxidant and antiproliferation properties, constitute the majority of polyphenolics in the pomace and are valuable for recovery. In the present study, we developed a novel procedure with sodium bicarbonate assisted extraction (SBAE) to recover ellagic acid from raspberry wine pomace. Key parameters in the procedure, i.e., sodium bicarbonate concentration, temperature, time and solid/liquid (S/L) ratio, were investigated by single factor analysis and optimized subsequently by Response Surface Methodology (RSM). Optimal parameters for the SBAE method here were found to be 1.2% (w/v) NaHCO3, 1:93 (w/v) S/L ratio, 22 min and 100 °C. Under these conditions, the ellagic acid yield was 6.30 ± 0.92 mg/g pomace with an antioxidant activity of 79.0 ± 0.96 μmol Trolox eq/g pomace (DPPH assay), which are 2.37 and 1.32 times the values obtained by extraction with methanol–acetone–water solvent, respectively. The considerable improvement in ellagic acid extraction efficiency could be highly attributed to the reactions of lipid saponification and ellagitannin hydrolysis resulted from sodium bicarbonates. The present study has established an organic solvent-free method for the extraction of ellagic acid from raspberry wine pomace, which is feasible and practical in nutraceutical applications.

The possibility to work by using small objects, under the microscopic view, it is a challenge. The force's spectrum differs from that of the macroscopic scale, and many unexpected force vectors will appear. A sharp tip must be the ideal pressure vector on different microscopic small object to push, roll, and brake and so on. The positioning of this tip must be a very precise one, comparatively with the working area dimensions and we consider that 10 microns can be a reasonable measuring unit for these moves that are executed into a specific way in these kinds of cases. The mechatronic system designed to actuate the tip, becomes this paper subject. Indisputable we will talk about an actuator with one or more than one freedom degrees. Mechanical aspects are secondary, but necessary to understand the impact of electrical parameters on the minimum possible unit of movement. Electronic control refers to a switching and PWM signal, through a software that develops both the effective part of generating the control signal and the HMI interface through a touch-screen. The aim of this paper was to determine the minimum micrometric travel distance that can be obtained with a 12-bit PWM signal generated by a Raspberry mini-computer [ABSTRACT FROM AUTHOR]

Raspberries (Rubus idaeus L.) are important vegetatively propagated fruit plants. They are used as food, as medicinal plants, and in pharmaceutical and cosmetic industry. For the protection of intellectual property rights, it is very important to have efficient methods that enable fast and accurate identification of cultivars. Raspberry cultivars can be differentiated in two ways: on the phenotypic level with morphological descriptors and on the molecular level with molecular markers. Among the frequently used are SSRs, which are highly informative, easy to score and have a good genome coverage. Considering 19 previously published SSR molecular markers, we selected the most specific loci for each selected genotype and developed specific fingerprint for each of the genotypes included in the study: four red raspberry cultivars ('Polka,' 'Glen Ample,' 'Meeker,' 'Rose de Côte d'Or') and two genotypes named Sicoly and Dieffenbach. The aim of our investigation was to demonstrate the differentiation between studied raspberry genotypes by specific amplified fragment patterns, which were unique for each genotype and were based on simple sequence repeats (SSRs). Furthermore, the developed fingerprints were successfully tested on randomly chosen, unknown genotypes. [ABSTRACT FROM AUTHOR]

In Romania, the most well-known non-wood forest products (NWFPs) are represented by forest fruits, edible mushrooms and medicinal plants. The aim of this paper is to present a synthesis of forest fruits present in Vaslui County. Therefore, the most important forest fruits that were found in this area are: the raspberry (Rubus idaeus), the dog-rose (Rosa canina), the hawthorn (Crataegus sp.), the wild apple (Malus sylvestris), the Cornelian cherry (Cornus mas), cranberries (Ribes nigrum L.), the bird cherry (Prunus padus) and acorns (Quercus sp). The most important forest fruits from Vaslui County were determined by means of an analytical hierarchy process (AHP) developed by Thomas Saaty. The analytical hierarchy process has emphasized raspberries and dog-roses as the most requested and, at the same time, the most important forest fruits from Vaslui County. Using the analytic hierarchy process in estimating the importance of fruit species from a certain County proved to be an efficient method of classifying them. [ABSTRACT FROM AUTHOR]

In the COVID-19 or Severe Acute Respiratory Syndrome Corona virus-2 is an extremely transmissible virus that is discharged through breathing droplets released from an infected individual who is talking, sneezing, or coughing. Close interaction with a person infected or through touching a contaminated surface and object can spread the virus rapidly. As of now, there is no vaccine to combat the COVID-19, and the best way to protect the person from a virus is to avoid being exposed to it. Wearing a facemask that covers the nose and mouth in a public setting and repeatedly cleansing of hands or the use of at least 70% alcohol-based disinfectants is a practice to avoid virus exposure. Deep Learning technology has demonstrated its achievement in recognition and classification by processing images. In this paper uses deep learning techniques that identify if the person is wearing a facemask or not and check the temperature of the person. The collected image data contains 20,000 images, uniformly crop images in 224x224 pixels, and attained an accuracy rate of 97% during the training of the model. The developed system is implemented using Python and OpenCV through TensorFlow that recognizes persons wearing a facemask or not wearing and temperature. It signals an alarm and captures facial images upon detecting persons not wearing a mask and does not observe maintain the temperature. It is beneficial in combating the spread of the virus and avoiding contact with the virus. [ABSTRACT FROM AUTHOR]

The biochemical composition of raspberry fruit was studied at two different planting distances. Their qualitative characteristics were determined. The impact of an individual technological element on the studied indicators was analysed. The experiment included raspberry cultivars, such as 'Willamette', 'Meeker', 'Samodiva' and a candidate cultivar 'Magdalena' from the collection plantation of RIMSA-Troyan. The planting material was produced in vitro. The planting distances were: 0.30 m and 0.50 m in the intra row spacing and 3.00 m in the row spacing. The highest amount of anthocyanins (38.87 mg) and total polyphenols (263.37 mg%) were reported for 'Willamette' fruit at 0.50 m planting distances. The highest antioxidant activity was reported in 'Willamette' fruit (7750.00 µmol TE/100 g) (at 0.30 m) and 'Samodiva' (6625.00 µmol TE/100 g) (at 0.50 m). The sensor rating is in the range from 4.63 ('Willamette' - 0.50 m) to 4.93 ('Magdalena' candidate cultivar - 0.30 m). [ABSTRACT FROM AUTHOR]

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Introduction Rubus idaeus (R. idaeus) is an edible fruit that contains numerous significant bioactive compounds that hold important biological properties and are categorized as nutraceuticals owing to the health benefits it imparts including decreasing the risk of chronic diseases like diabetes mellitus, cancer, heart disease, and many other diseases. The objective of the present research was to explore the antimicrobial, antioxidant, and anti-inflammatory characteristics of the aqueous raspberry extract through in vitro assays. Materials and methods R. idaeus aqueous extract was prepared and examined for its antimicrobial activity against Streptococcus mutans (S. mutans) bacteria and Candida albicans (C. albicans) fungi using the agar-well diffusion method, and the antioxidant activity was evaluated using the DPPH (2, 2-Diphenyl-1-picrylhydrazyl hydrate) radical scavenging assay and the hydrogen peroxide radical scavenging assay. The anti-inflammatory activity of the prepared extract was investigated using bovine serum albumin (BSA) and egg albumin denaturation assays.  Results R. idaeus fruit extract displayed strong antimicrobial activity at a higher concentration of 100 µL with a 26 mm zone of inhibition against Streptococcus mutans and 24 mm for Candida albicans. The extract showed 87.42% hydrogen peroxide free radical scavenging activity and inhibited 91.12% DPPH free radicals at the highest concentration of 50 µg/mL. The extract showed effective anti-inflammatory activity by preventing the denaturation of bovine serum albumin (80%) and egg albumin proteins (77%) at the highest concentration of 50 μg/mL. The free radical scavenging activity positively correlates with the increased concentration of the prepared extract against DPPH and hydrogen peroxide free radicals, thus showing the raspberry extract's potent antioxidant activity. Similarly, the anti-inflammatory assay result shows that the prepared raspberry aqueous extract has excellent anti-inflammatory activity by preventing the denaturation of bovine serum albumin and egg albumin protein in a dose-dependent manner. Conclusion The meticulously prepared raspberry extract exhibited noteworthy antimicrobial, antioxidant, and anti-inflammatory characteristics, and owing to its astounding therapeutic benefits it holds a tremendous promise as a natural alternative in the field of oral medicine especially in the management of oral mucosal lesions, oral potentially malignant lesions such as lichen planus and leukoplakia, candidiasis, oral cancer and oral mucositis. Further animal studies and clinical trials are recommended to fully reap the therapeutics potential of raspberry extract., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2024, Gomathi et al.)

Application of an aroma extract dilution analysis (AEDA) to the volatiles isolated from raspberry fruits by solvent extraction and solvent-assisted flavour evaporation (SAFE) resulted in 40 odour-active compounds with flavour dilution (FD) factors between 1 and 4096. Among the most potent odorants were violet-like smelling β-ionone (FD factor 4096), fruity smelling methyl 3-methylbutanoate (1024), baked-apple-like smelling β-damascenone (1024), raspberry-like smelling raspberry ketone (128), and floral, raspberry-like smelling α-ionone (64). These five odorants were subsequently monitored during processing of raspberry fruits to freeze-dried fruit foam. Major losses occurred during separation of the pulp from the seeds and during the final freeze-drying step. It was shown that the pulp fraction directly adherent to the seeds contained higher odorant concentrations than the outer parts of the pulp, thus losses associated with the removal of the seeds can be minimised by increasing the efficiency of the separation. Losses associated with the freeze-drying process could be reduced using microwave-assisted freeze drying instead of conventional freeze drying. Higher amounts of potato protein and maltodextrin used as foaming agent and foam stabiliser, respectively, reduced the odorant recoveries in the dried foams. Only a small part of the odorants not recovered in the dried fruit foams was found in the condensate. [ABSTRACT FROM AUTHOR]

Drosophila suzukii (Matsumura) is a major global pest of soft fruit crops. Based on field observations, we tested in the laboratory whether sweet alyssum (Lobularia maritima (L.)) flower volatiles and their major constituent compounds, acetophenone and benzaldehyde, repelled D. suzukii flies. Volatiles from cut flowers and acetophenone reduced numbers of D. suzukii attracted to raspberries, and acetophenone reduced numbers of larvae in the raspberries. Testing of sweet alyssum plantings or dispensing acetophenone to repel D. suzukii in fields and lower fruit infestation should be conducted. [ABSTRACT FROM AUTHOR]

The growth of agricultural mechanization has promoted an increase in raspberry production, and for this reason, the best postharvest storage and processing techniques capable of maintaining the health beneficial properties of these perishable berry fruits have been widely studied. Indeed, raspberries are a rich source of bioactive chemical compounds (e.g., ellagitannins, anthocyanins, and ascorbic acid), but these can be altered by postharvest storage and processing techniques before consumption. Although there are clear differences in storage times and techniques, the content of bioactive chemical compounds is relatively stable with some minor changes in ascorbic acid or anthocyanin content during cold (5 °C) or frozen storage. In the literature, processing techniques such as juicing or drying have negatively affected the content of bioactive chemical compounds. Among drying techniques, hot air (oven) drying is the process that alters the content of bioactive chemical compounds the most. For this reason, new drying technologies such as microwave and heat pumps have been developed. These novel techniques are more successful in retaining bioactive chemical compounds with respect to conventional hot air drying. This mini-review surveys recent literature concerning the effects of postharvest storage and processing techniques on raspberry bioactive chemical compound content. [ABSTRACT FROM AUTHOR]

Spray coverage may influence the efficacy of insecticides targeting the invasive vinegar fly Drosophila suzukii (Matsumura), a primary pest of raspberries and blackberries. In commercially managed caneberries, spray coverage is typically lowest in the inner and lower plant canopy, regions that overlap with higher levels of adult D. suzukii activity. To understand how spray coverage of fruit impacts efficacy against D. suzukii , laboratory bioassays were conducted using raspberries. In laboratory bioassays, higher spray coverage did not impact larval infestation rates but did increase adult mortality, indicating that flies can avoid a lethal dose of insecticide when applications do not achieve adequate coverage. We also evaluated how carrier water volume impacts spray coverage patterns throughout the canopy of raspberry and blackberry plants using both airblast and CO2 backpack sprayers. Increasing carrier water volume generally improved spray coverage in the lower plant canopy. However, effects in the upper plant canopy were inconsistent and varied between sprayer types. In addition to carrier water volume, other approaches, including adjusting the pesticide sprayer equipment used and/or sprayer calibration, should also be explored to improve coverage. Growers should evaluate spray coverage in their caneberries to identify and troubleshoot coverage issues. Results from this study indicate that taking the time to optimize this aspect of pesticide application may improve chemical management of D. suzukii and will likely also improve control of other important caneberry pests. [ABSTRACT FROM AUTHOR]

Background: A key challenge for manufacturers of pro-health food containing active probiotic microorganisms is to develop a product with attractive sensory features along with maintenance of declared number of microorganisms during storage and transfer by alimentary tract. Results: The highest concentration of polyphenols was observed in snacks without an additive of probiotics as well as those with an additive of L. rhamnosus and B. animalis bacteria and concentration of these compounds increased by 9.5% during six months of storage. None of the products distinguished itself in the sensorial assessment although each was assessed positively. The number of microorganisms was stable and comparatively high during six months of storage at a room temperature and in cooling conditions (108 cfu/g). In the digestion model, an influence of aggressive digestion conditions was examined in the alimentary tract on the number of microorganisms, which allowed to arrange strains from the most resistant (S. boulardii) to the most sensitive (B. breve). It must be noted that currently on the market there is no available snack containing probiotic yeast as well as there is no literature data on works on such formulation of food. Conclusions: In the newly developed snack made of chocolate, in which sugar has been replaced with maltitol, a rawmaterial was added in the form of raspberry, prebiotic in the form of inulin and a strain of probiotic bacteria, including the unprecedented so far S. boulardii, which stands a high chance to occupy a good place on the market of functional food. [ABSTRACT FROM AUTHOR]

A new record of Rubus (Rosaceae) for Ecuador is described: R. rosifolius, a climbing shrub introduced and naturalized in the Ecuadorian Andes for its edible fruits. We provide an updated key for the genus Rubus in Ecuador, a detailed description of the species, and its distribution. In addition, photographs of this species and complete illustrations are included. [ABSTRACT FROM AUTHOR]

The research was carried out in the years 2015–2018 on the commercial plantations of a horticultural farm. The analysis focused on the yields of two raspberry cultivars: ‘Polana’ and ‘Polka’, factoring in the influence of cultivation methods (in the ground; in the beds with an agrotextile), soil classes (IIIa; IVb), and plantation age (2–5 and 6–10 years). The commercial yields of fruit intended for processing were determined. The economic efficiency analysis of the production of both varieties was carried out. It was shown that regardless of the factor under scrutiny, higher results (in t·ha–1) were obtained from the ‘Polana’ variety. There were differences in the yields of the two varieties depending on the factors. In the case of both varieties, higher results were obtained in traditional cultivation and in class IIIa soil. The plantation age caused only slight differences in the yields of both varieties. The analyses showed statistical significance only in the case of the impact of the cultivation method on the yields of the ‘Polana’ cultivar. Regardless of the variety, the value of production decreased year after year, which resulted mainly from the downward trend in raspberry prices.

Raspberries are rich in phytochemicals with health benefits. Our previous research has shown raspberry extracts (RE) can promote longevity in Caenorhabditis elegans. The objective of this study was to determine whether the extension of lifespan was regulated by oxidative stress, and to explore the underlying mechanisms of action. The results showed that RE could ameliorate oxidative stress, which improved antioxidant enzyme activity and enhanced resistance to paraquat. Meanwhile, treatment with RE could down-regulate the overexpression of reactive oxygen species and affect the expression of antioxidant related genes. However, administration with RE abolished the extension of the lifespan of skn-1(zu135) mutants, and promoted the migration of SKN-1 into the nucleus, which inhibited the expression of skn-1 downstream gene. In conclusion, RE could ameliorate oxidative stress in C. elegans via the SKN-1/Nrf2 pathway. This study provided the theoretical basis to explore the phytochemicals in the prevention of aging regulated by oxidative stress.

MicroRNAs (miRNAs) are a class of small endogenous RNAs that play important regulatory roles in plants by negatively affecting gene expression. Studies on the identification of miRNAs and their functions in various plant species and organs have significantly contributed to plant development research. In the current study, we utilized high-throughput sequencing to detect the miRNAs in the root, stem, and leaf tissues of raspberry (Rubus idaeus). A total of more than 35 million small RNA reads ranging in size from 18 to 35 nucleotides were obtained, with 147 known miRNAs and 542 novel miRNAs identified among the three organs. Sequence verification and the relative expression profiles of the six known miRNAs were investigated by stem-loop quantitative real-time PCR. Furthermore, the potential target genes of the known and novel miRNAs were predicted and subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway annotation. Enrichment analysis of the GO-associated biological processes and molecular functions revealed that these target genes were potentially involved in a wide range of metabolic pathways and developmental processes. Moreover, the miRNA target prediction revealed that most of the targets predicted as transcription factor-coding genes are involved in cellular and metabolic processes. This report is the first to identify miRNAs in raspberry. The detected miRNAs were analyzed by cluster analysis according to their expression, which revealed that these conservative miRNAs are necessary for plant functioning. The results add novel miRNAs to the raspberry transcriptome, providing a useful resource for the further elucidation of the functional roles of miRNAs in raspberry growth and development.

Emerging evidence suggests that anthocyanins prevent type 2 diabetes (T2D), however, its mechanisms remain elusive. Herein, we sought to elucidate the antihyperglycemic mechanism of pelargonidin-3-O-glucoside (Pg3G) extracted from wild raspberry based on RNA-sequence. Our study showed that Pg3G ameliorated glucose intolerance and insulin resistance in db/db mice. Besides, Pg3G improved serum lipid profiles and attenuated hepatic dysfunction. RNA-sequence analysis showed that Pg3G contributed to 301 genes upregulated and 269 genes downregulated. Further GO, KEGG and GSEA analysis indicated that Pg3G led to differential gene expressions enriched in glucose and lipid metabolism by upregulation of Elovl7, C1ql3, Cybb, Cytip, Src, Pdk4 and downregulation of Fasn, Aacs, Srebp1c, Mlxipl, Sorbs3, Gck, Slc2a4. RT-PCR validation of selected genes exhibited a positive correlation between RNA-sequence and RT-PCR results. Taken together, this study demonstrated that Pg3G attenuated T2D by regulating glucose and lipid metabolism, which implicates the potential for T2D therapeutics.

Raspberry (Rubus idaeus L.), known as one of the famous healthy fruits an d are consumed fresh or processed products all over the world. The antioxidation activity of raspberry fruits as well as leaves have been widely investigated. To better understand the metabolite accumulation mechanisms and to develop different functional cultivars, we performed a non-targeted metabolomics analysis using LC-MS/MS to investigate the contents of existing components from three raspberry cultivars, Autumn Britten, Autumn Bliss, and Red Autumn leaves, respectively. The results show multiple differentially accumulated metabolites among three cultivars, especially for the lipids (α-linolenic acid and eicosatetraenoic acid), amino acids and their derivatives (L-cysteine, Phenylalanine), flavonoids (Kaempferol 3-O-rhamnoside-7-O-glucoside, Quercetin 3-glucoside), and vitamins (Biotin, Thiamine, Vitamin K2), etc. The in vitro cellular antioxidant activities of three raspberry cultivars leaves ethanol extracts (RLEE) were also characterized. Through comparison the superoxide dismutase (SOD), glutathione (GSH), catalase (CAT), and reactive oxygen species (ROS) levels before or after RLEE protection of L929 fibroblast cells upon excessive UVB exposure, we evaluated the antioxidation potentials for all three cultivar RLEEs. It turns out the raspberry Autumn Britten leaf extract holds the greatest potential for protecting the L929 fibroblast cells from UVB induced damage. Our study provides theoretical support for screening of active metabolites from three raspberry cultivars leaves, spanning metabolites’ accumulation to cell damage protection, which could be used to refine bioactivity assessment for different raspberry cultivars suitable for antioxidant products extraction.

Raspberry promotes adipose tissue browning and increases lipid oxidation, but its effect on intramyocellular lipid accumulation and insulin sensitivity is unclear. This study aimed to evaluate the protective role of raspberry on high-fat diet (HFD)-induced skeletal muscle lipid accumulation and insulin resistance in mice. Mice were fed a control diet or a HFD diet with or without 5% raspberry for 12 weeks. Dietary raspberry alleviated skeletal muscle inflammatory response by decreasing the expression of pro-inflammatory cytokines and p65 phosphorylation, a key mediator of inflammatory nuclear factor κB (NF-κB) signaling. Meanwhile, raspberry reduced muscle triglyceride content and improved insulin signaling in HFD mice. Raspberry intake increased mitochondrial biogenesis and phosphorylation of AMP-activated protein kinase (AMPK) α impaired due to HFD. Our results suggest that raspberry reduces lipid accumulation and improves insulin sensitivity in skeletal muscle of HFD-fed mice, which is associated with suppressed inflammation and increased mitochondrial biogenesis and AMPKα activity.

Abstract Background The changing climate is altering timing of key fruit ripening processes and increasing the occurrence of fruit defects. To improve our understanding of the genetic control of raspberry fruit development an enhanced genetic linkage map was developed and used to examine ripening phenotypic data. Results In this study we developed an enhanced genetic linkage map for the raspberry cvs. Glen Moy x Latham reference mapping population using genotyping by sequencing (GbS). Alignment to a newly sequenced draft reference genome of red raspberry, cultivar (cv.) Glen Moy, identified 8019 single nucleotide polymorphisms (SNPs). After stringent filtering to take account of read coverage over all the progeny individuals, association with a single chromosome, heterozygosity and marker regression mapping, 2348 high confidence SNPs were retained and integrated with an existing raspberry genetic map. The linkage map contained many more SNPs segregating in Latham than in Glen Moy. This caused difficulties in quantitative trait loci (QTL) mapping with standard software and a novel analysis based on a hidden Markov model was used to improve the mapping. QTL mapping using the newly generated dense genetic map not only corroborated previously identified genetic locations but also provided additional genetic elements controlling fruit ripening in raspberry. Conclusion The high-density GbS map located the QTL peaks more precisely than in earlier studies, aligned the QTLs with Glen Moy genome scaffolds, narrowed the range of potential candidate genes to these regions that can be utilised in other populations or in gene expression studies to confirm their role and increased the repertoire of markers available to understand the genetic control of fruit ripening traits.