201. 疏肝健脾法对产后缺乳大鼠催乳素及其受体表达的影响研究.
- Author
-
陈宝艳, 甄玉花, 赵颖, 王淑平, and 孙雪
- Subjects
- *
CORTICOTROPIN releasing hormone , *MAMMARY glands , *CONNECTIVE tissues , *ADRENOCORTICOTROPIC hormone , *CHINESE medicine , *DENTAL amalgams - Abstract
Objective: To observe the effect of Shugan Jianpi methodon tlie histology. PRL and PRLR of mammary gland in rats with postpartum hypogalactia of liver stagnation and spleen deficiency syndrome. Methods: The rat model of postpartum hypogalactia of liver stagnationand spleen deficiency was reproduced by bromocriptine mesylate solution and chronic binding method. .After the drug was gavaged. HE staining and immunohistocheinistry were used to compare the effects of different interventions on mammary gland morphology and expression of prolactin and prolactin receptors in postpartum lactating rats. Serum and mammary gland PRL. serum GAS and the hypothalamic (The hypothalamic-pituitary-adrenal axis. HPA axis) related indicators were detected by enzyme-linked iimnuno sorbent assay (ELISA): serum corticotropin releasing hormone (CRH), serum adrenocorticotropic hormone (AC'TH). serum cortisol (COR). Results: Compared to the model group, the methoxychloropylamine group and Chinese medicine of high, medium and low dose group can promote the development of breast tissue to active lactation, which is characterized by acinar cavity filling, increased number of acinar epithelial cells, and deterioration of connective tissue and adipose tissue. At the same time, the methoxychloropylamine group and Chinese medicine can increase the expression of PRL and PRLR in breast tissue. The effect of Chinese medicine medium dose treatment group is better than the other groups, hi breast tissue, the relationship between PRL and PRLR is linearly related (1-0.845. P< 0.05). Compared to the model group, serum COR. CRH and AC'TH in the therapy group decreased significantly (P<0.05). while serum and mammary gland PRL and serum GAS level increased (P< 0.05). Conclusion: The mechanism of Shugan Jianpi method promoting lactation hi postparnun hypogalactia rats is closely related to increasing the expression of PRL and PRL receptor expression, regulating gastrointestinal function, inhibiting hyperactivity of HPA axis, and thus promoting the development of breast tissue morphology to active lactation. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF