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The New England cottontail (NEC) Sylvilagus transitionalis is strongly associated with shrubland and early successional habitat and is the only cottontail native to the U.S. Northeast. The distribution and abundance of young forest habitat and NEC populations have declined. The eastern cottontail (EC) Sylvilagus floridanus was introduced into the U.S. Northeast in the early 1900s and uses similar habitat as NEC, but is expanding in distribution and abundance. Little information exists on spatial use, survival, and competition in sympatric populations of NEC and EC. Understanding differences in population demographics may identify important factors or relationships influencing population trends and aid in developing effective management strategies. Our objectives were to quantify home range and core area sizes, annual survival rates, minimum population densities, and range overlap for sympatric populations of NEC and EC at four sites in Connecticut. We monitored spatial use and survival rates of 107 radio-collared rabbits over a 10-y period. Mean annual home ranges and core areas were 10.9 and 2.5 ha for NEC and 5.6 and 1.6 ha for EC. Overlap in home range and core areas was greater within species than between species (NEC-EC). For both species and sex, home range size expanded from winter to breeding seasons. Survival rates were greater for NEC than for EC at all four sites, with predation as the major cause of mortality for both species. Space-use patterns suggest that the potential for EC to interfere with NEC reproduction is limited and avoidance or resource partitioning between species in the same patch may be occurring.

The effects of agricultural landscape transformation and subsequent habitat associations have been little studied for the Agulhas long-billed lark (Certhilauda brevirostris), a regionally threatened species in the Overberg, Western Cape, South Africa. Point count surveys were conducted throughout the Overberg using distance sampling techniques. We calculated bird density and extrapolated this to a population range estimate of between 203 000 and 368 000 individuals. Using generalised linear modelling of habitat variables from the point counts, we found that the birds prefer short vegetation, regardless of habitat type or cover. Broad-scale habitat association patterns were examined using SABAP2 data to determine if reporting rate (a measure of abundance) was correlated with the proportion of natural habitat within each pentad, finding that reporting rates were lower for areas with large patches of natural vegetation and extensive road access. It remains inconclusive whether agricultural practices benefited Agulhas long-billed larks, or whether these practices mimic historical ecological processes, but the population appears secure and even increasing with the status quo.Keywords: agricultural transformation, Alaudidae, distance sampling, endemic species, fynbos, habitat selection

© 2019 - IOS Press and the authors. Dual-system estimation is a well-established approach for estimating an unknown population size from two independent but imperfect counts of the population. In this paper we develop the estimation framework for using a coverage survey and population census as the two sources and combining with ratio estimation to produce a set of population estimates. Adjustments are developed to correct for a failure of the key assumptions of homogeneity and independence that under-pin dual-system estimation using an external count of the number of households. The issue of over-count within the census is also discussed and a bootstrap approach to variance estimation is proposed. A comprehensive set of simulation results are presented to support the decision to implement the framework to estimate the population following the 2011 Census of England and Wales; and the implementation to the estimation of census coverage in 2011 is discussed.

Freshwater turtles often utilize basking habitats, allowing researchers to obtain population estimates and relative abundances from visual observations via spotting scopes in addition to other traditional trapping methods. Emerging technologies, such as camera trapping with wildlife trail cameras have been extensively utilized in other taxa, primarily mammals and in reptiles such as terrestrial tortoises, but to a lesser extent for monitoring freshwater turtles. Given their ability to bask, combining readily available non-invasive camera traps with standardized platforms may aid researchers study freshwater turtle populations and basking behavior. We assessed this method by deploying a novel artificial basking platform design in tandem with camera traps for weekly monitoring of turtles at a small semi-urban pond in central North Carolina for six months (April to September 2018). Basking behavior was documented with 1098 observations, with the number of turtles utilizing platforms varying according to season, and overall peak use during late spring and early fall. We also noted shifts in artificial basking structure use by species, with Painted turtles, Chrysemys picta, replacing Yellow-bellied slider turtles, Trachemys scripta, as the dominant basking species over time. Conservation managers should consider using both platforms and trail cameras, for monitoring of freshwater basking turtle populations and as a metric for turtle presence or for detailed studies of behavior.

Walleyes Sander vitreus are an important sport fish found throughout the northern Great Plains of North America. Periods of increased precipitation across the Great Plains can result in new or expanded lakes that contain unexploited fish populations. We assessed angler exploitation of a previously unexploited walleye population to estimate exploitation rates, subsequent changes in walleye abundance, and changes in angler behavior. This research provides an opportunity to add to the limited body of information concerning walleye exploitation and exploitation of unexploited populations. Hazeldon Lake, South Dakota contained an unexploited walleye population and was opened to angling on 15 July 2006. Initial exploitation was high, estimates exceeded 16% after two days and 75% after 16 days. Anglers harvested an estimated 6868 walleyes during 15 July - 31 August 2006; this harvest represented 79% of the pre-opener population estimate of 8661. The 2007 spring population estimate was 2436 fish and represented a 72% decline from the 2006 estimate. Gill net catch per unit effort (CPUE) averaged 30.0 fish net−1 prior to fishing in 2006; the 2007 CPUE was 76% lower at 7.2 fish net−1. A lack of recruitment resulted in no substantial increases in relative abundance from 2007-2017. Fishing pressure was high when the lake first opened to fishing. However, the number of anglers and exploitation rates declined once walleye catch and harvest rates dropped off. Our results suggest that walleye populations in new or expanded waters located in the northern Great Plains can be extremely vulnerable to angling but when catch rates drop off few anglers will continue to fish a given water; instead moving to other waters potentially having higher walleye catch rates.

The latest population estimates released by Statistics South Africa indicate that 25.03% of all deaths in 2017 in South Africa were AIDS-related. Along with these results, it is also reported that 7.06% of the population were living with HIV, with the HIV-prevalence among youth (aged 15–24) at 4.64% for 2017 (STATSSA. (2018). Retrieved from Statistics South Africa: http://www.statssa.gov.za/publications/P0302/P03022017.pdf). The data used in the study contained information related to the risk-taking behaviours associated with the sexual activity of entering first-year students at the University of the Western Cape. In this study, a logistic regression modelling procedure was carried out on those students that were determined to be sexually active, therefore, in the modelling procedure significant risk behaviours of sexually active first-year students could be identified. Of the 14 variables included in the modelling procedure, six were found to be significantly associated with sexually active students. The significant variables included; the age and race of the student, whether the student had ever taken an HIV test, the importance of religion in influencing the sexual behaviour of the student, whether the student consumed alcohol and lastly whether the student smoked. This study further investigated the impact of introducing sample weighting, bootstrap sampling as well as variable selection methods into the logistic regression modelling procedure. It is shown that incorporating these techniques into the modelling procedure produces logistic regression models that are more accurate and have an increased predictive capability. The bootstrapping procedure is shown to produce logistic regression models that are more accurate than those produced without a bootstrap procedure. A comparison between 200, 500 and 1000 bootstrap samples is also incorporated into the modelling procedure with the models produced from 200 bootstrap samples shown to be just as accurate those produced from 500 or 1000 bootstrap samples. Of the five variable selection methods used, it is shown that the Newton–Raphson and Fisher methods are unreliable in producing logistic regression models. The forward, backward and stepwise variable selection methods are shown to produce very similar results.

Listed as ‘threatened’ under the US Endangered Species Act in 1993, the spectacled eider Somateria fischeri population in western Alaska, USA, has since rebounded, prompting an assessment of their suitability for delisting. This assessment, however, is limited by aerial-based population estimates that are incompletely corrected for unobserved eiders. Notably, aerial counts of eiders are corrected with a visibility correction factor (VCF), calculated as the ratio of ground-based nest counts to aerial pair counts, which disregards spatial variation in eider density. Accordingly, we (1) stratified our study area into zones of low, medium, and high eider density, (2) developed density-adjusted VCFs for each stratum, (3) evaluated the influence of several ecological factors on VCFs, and (4) estimated eider population size using our density-adjusted VCFs. For the low-density stratum, we estimated a VCF (± SE) of 1.35 ± 0.15, indicating that aerial counts of eider pairs closely matched ground counts of nests. In medium and high-density strata, VCFs increased to 2.46 ± 0.17 and 3.09 ± 0.19, respectively, suggesting that aerial detection decreased as eider densities increased. VCFs also increased for surveys that occurred late relative to nest initiation. Population estimates produced with our density-adjusted VCFs were 42% (5580 eiders) lower, on average, than those produced with the traditional VCF, which is currently used. Such large differences underscore the importance of accounting for density when correcting counts for incomplete detection, and, for threatened species such as spectacled eiders, may determine whether populations retain protected status.

This work proposes to use the photographic capture-recapture technique associated with the GPS positioning (Global Positioning System) to estimate the population and to evaluate the movement of free-roaming dogs in public spaces. An observer on a motorcycle traveled all the streets of Jardim Ipanema and part of Dona Amelia neighborhoods in Aracatuba, SP, totaling a ​​1.3 Km2 area covered on two days, with a one-week interval between them. The free-roaming dogs were photographed and their geographical locations were determined using GPS, and this geographic record was then used to estimate the spatial and temporal distribution of free-roaming dogs in the neighborhood. A total of 77 dogs were found on the streets, 21 of which were photographed more than once (photo-recaptured). The number of free-roaming dogs was estimated from the capture rate using a linear regression model, revealing a population of 76 animals, corresponding to 7.5% of the canine population living in the neighborhood. The results indicated a higher concentration of free-roaming dogs in a certain neighborhood region that has unpaved streets and most houses had no walls. Furthermore, the highest number of dogs was observed early in the day (between 7 and 9 a.m.) and that, among the dogs observed moving through the neighborhood streets, the longest distance traveled was 520m. It is concluded that the proposed methodology is an efficient tool and can be used when planning public health activities.

In order to be heard over the low-frequency energy of a loud orchestra, opera singers adjust their vocal tracts to increase high-frequency energy around 3,000 Hz (known as a “singer's formant”). In rap music, rhymes often coincide with the beat and thus may be masked by loud, low-frequency percussion events. How do emcees (i.e., rappers) avoid masking of on-beat rhymes? If emcees exploit formant structure, this may be reflected in the distribution of on- and off-beat vowels. To test this prediction, we used a sample of words from the MCFlow rap lyric corpus (Condit-Schultz, 2016). Frequency of occurrence of on- and off-beat words was compared. Each word contained one of eight vowel nuclei; population estimates of each vowel's first and second formant (F1 and F2) frequencies were obtained from an existing source. A bias was observed: vowels with higher F2, which are less likely to be masked by percussion, were favored for on-beat words. Words with lower F2 vowels, which may be masked, were more likely to deviate from the beat. Bias was most evident among rhyming words but persisted for nonrhyming words. These findings imply that emcees use formant structure to implicitly or explicitly target the intelligibility of salient lyric events.

Effective management of the threatened Antillean manatee (Trichechus manatus manatus) in Puerto Rico requires reliable estimates of population size. Estimates are needed to assess population responses to management actions, and whether recovery objectives have been met. Aerial surveys have been conducted since 1976, but none adjusted for imperfect detection. We summarize surveys since 1976, report on current distribution, and provide population estimates after accounting for apparent detection probability for surveys between June 2010 and March 2014. Estimates in areas of high concentration (hotspots) averaged 317 ± 101, three times higher than unadjusted counts (104 ± 0.56). Adjusted estimates in three areas outside hotspots also differed markedly from counts (75 ± 9.89 versus 19.5 ± 3.5). Average minimum island-wide estimate was 386 ± 89, similar to the maximum estimate of 360 suggested in 2005, but fewer than the 700 recently suggested by the Puerto Rico Manatee Conservation Center. Manatees were more widespread than previously understood. Improving estimates, locally or island-wide, will require stratifying the island differently and greater knowledge about factors affecting detection probability. Sharing our protocol with partners in nearby islands (e.g., Cuba, Jamaica, Hispaniola), whose populations share genetic make-up, would contribute to enhanced regional conservation through better population estimates and tracking range expansion.El manejo efectivo del manatí antillano amenazado en Puerto Rico requiere estimados de tamaños de poblaciónes confiables. Dichas estimaciones poblacionales son necesarias para evaluar las respuestas a las acciones de manejo, y para determinar si los objetivos de recuperación han sido alcanzados. Se han realizado censos aéreos desde 1976, pero ninguno de ellos han sido ajustados para detecciones imperfectas. Aquí resumimos los censos desde 1976, actualizamos la distribución, y reportamos los primeros estimados poblacionales ajustados para la probabilidad de detección aparente en los censos de Junio 2010 a Marzo 2014. Las estimaciones poblacionales en áreas de mayor concentración del manatí promedió 317 ± 103, tres veces más abundante que los conteos sin ajuste (104 ± 0.56). Las estimaciones poblacionales en tres áreas fuera de las áreas de mayor concentración del manatí también fueron marcadamente diferentes (75 ± 9.89 vs 19.5 ± 3.5). El estimado mínimo poblacional en la isla entera fue de 386 ± 89, similar al estimado máximo de 360 sugerido en el año 2005, pero menor a los 700 sugeridos recientemente por el Centro de Conservación de Manatíes de Puerto Rico. Documentamos que el manatí tiene una distribución más amplia de lo que se sabía con anterioridad. El mejoramiento de los estimados poblacionales locales o a nivel de isla requerirá que se estratifique a la isla en forma diferente y que se investiguen los factores que influencian a la probabilidad de detección. Compartir protocolos como este con colaboradores de islas vecinas (por. ej., Cuba, Jamaica, Española), cuyas poblaciones de manatíes comparten material genético, contribuiría a la conservación regional mediante mejores estimaciones poblacionales y monitoreo de la expansión de su ámbito doméstico.

The majority of countries in Africa and nearly one-third of all countries require mortality models to infer the complete age schedules of mortality that are required to conduct population estimates, projections/forecasts, and other tasks in demography and epidemiology. Models that relate child mortality to mortality at other ages are important because almost all countries have measures of child mortality. A general, parameterizable component model (SVD-Comp) of mortality is defined using the singular value decomposition and calibrated to the relationship between child or child/adult mortality and mortality at other ages in the observed mortality schedules of the Human Mortality Database. Cross-validation is used to validate the model, and the predictive performance of the model is compared with that of the log-quadratic (Log-Quad) model, which is designed to do the same thing. Prediction and cross-validation tests indicate that the child mortality–calibrated SVD-Comp is able to accurately represent the observed mortality schedules in the Human Mortality Database, is robust to the selection of mortality schedules used for calibration, and performs better than the Log-Quad model. The child mortality–calibrated SVD-Comp can be used where and when child mortality is available but mortality at other ages is unknown.

Objectives This study aims to evaluate the radiation exposure to patients undergoing an abdominopelvic or a chest and abdominopelvic (trunk) CT examination and to assess compliance to imaging referral guidelines. Methods To this end, 357 standard abdominopelvic and trunk CT scans were collected from 8 Belgian institutions in 2011 and 2015. Effective dose (E) and collective dose were calculated using CT dose descriptors (CTDIvol and DLP), which were obtained from dose reports generated by the CT scanner. Subsequently, these CT requests were compared against the national referral guidelines to assess compliance. Population estimates for dose and compliance were obtained using multilevel linear regression and generalized estimating equation models. Results Between 2011 and 2015, a significant decrease of 28% in CTDIvol and DLP was found for abdominopelvic CT. The corresponding E decreased by 27%, and the collective dose by 23%. For trunk CT, a non-significant decrease of 6% was found in CTDIvol and a significant decrease of 14% in both DLP and E. However, the collective dose of trunk examinations increased by 39%. In 2011, 24% of the abdominopelvic examinations were not compliant with the guidelines, whereas this percentage dropped to 17% in 2015. Non-justified examinations accounted for 8% and 12% of all trunk CT scans in 2011 and 2015, respectively. Conclusion Between 2011 and 2015, there was a decrease in dose for patients undergoing abdominopelvic or trunk CT in Belgium. However, a fraction of the estimated doses are linked to unnecessary CT examinations, which should be avoided.

Data integration is now common practice in official statistics and involves an increasing number of sources. When using multiple sources, an objective is to assess the unknown size of the population. To this aim, capture-recapture methods are applied. Standard capture-recapture methods are based on a number of strong assumptions, including the absence of errors in the integration procedures. However, in particular when the integrated sources were not originally collected for statistical purposes, this assumption is unlikely and linkage errors (false links and missing links) may occur. In this article, the problem of adjusting population estimates in the presence of linkage errors in multiple lists is tackled; under homogeneous linkage error probabilities assumption, a solution is proposed in a realistic and practical scenario of multiple lists linkage procedure.

The red fox inhabits much of North America and is common in northern Europe and Russia. In many places, especially the midwestern states, hunting and trapping regulations are based on the number of foxes in the population during a given year in a particular region. Another approach to censusing foxes is simply to develop an index to the population and determine whether the population is up or down in a given year. Population estimates are best derived through a survey of active dens during the pup-rearing season. If the researcher or wildlife manager is not particularly interested in density estimates, but only in relative changes in population levels, several methods have been used to develop indices. Place scent stations consisting of a capsule of suitable commercial scent along unpaved or secondary roads at 0.3-mi intervals.

O presente traballo recolle os resultados obtidos en prospeccións de poboación de lobo (Canis lupus), abordadas en 2019 e 2020 nunha área de 2.900 km2 situada a nordeste de Galicia, onde o último censo oficial cifrou en 6 grupos a presenza reprodutora da especie. Ditas prospeccións sitúan a poboación potencialmente reprodutora nun mínimo de 22 grupos, en 16 dos cales (72.7%) foi posible confirmar reprodución. Estes rexistros poñen en destaque a necesidade de actualización das estimativas de poboación da especie e, traducidos a densidade de poboación, sitúan o nordeste de Galicia cuns valores de densidade estimada dos máis elevados en toda a área de distribución mundial da especie (4.99 – 11.0 ex. /100 km2).

Background Many individuals born with congenital heart defects (CHD) survive to adulthood. However, population estimates of CHD beyond early childhood are limited in the U.S. Objectives To estimate the percentage of individuals aged 1-to-64 years at five U.S. sites with CHD documented at a healthcare encounter during a three-year period and describe their characteristics. Methods Sites conducted population-based surveillance of CHD among 1 to 10-year-olds (three sites) and 11 to 64-year-olds (all five sites) by linking healthcare data. Eligible cases resided in the population catchment areas and had one or more healthcare encounters during the surveillance period (January 1, 2011-December 31, 2013) with a CHD-related ICD-9-CM code. Site-specific population census estimates from the same age groups and time period were used to assess percentage of individuals in the catchment area with a CHD-related ICD-9-CM code documented at a healthcare encounter (hereafter referred to as CHD cases). Severe and non-severe CHD were based on an established mutually exclusive anatomic hierarchy. Results Among 42,646 CHD cases, 23.7% had severe CHD and 51.5% were male. Percentage of CHD cases among 1 to 10-year-olds, was 6.36/1,000 (range: 4.33-9.96/1,000) but varied by CHD severity [severe: 1.56/1,000 (range: 1.04-2.64/1,000); non-severe: 4.80/1,000 (range: 3.28-7.32/1,000)]. Percentage of cases across all sites in 11 to 64-year-olds was 1.47/1,000 (range: 1.02-2.18/1,000) and varied by CHD severity [severe: 0.34/1,000 (range: 0.26-0.49/1,000); non-severe: 1.13/1,000 (range: 0.76-1.69/1,000)]. Percentage of CHD cases decreased with age until 20 to 44 years and, for non-severe CHD only, increased slightly for ages 45 to 64 years. Conclusion CHD cases varied by site, CHD severity, and age. These findings will inform planning for the needs of this growing population.

Burrowing seabirds are important in commercial, ecological and conservation terms. Many populations are in flux owing to both negative and positive anthropogenic impacts, but their ecology makes measuring changes difficult. Reliably recording key metrics, the proportion of burrows with breeding pairs, and the success of breeding attempts, requires burrow-level information on occupancy. We investigated the use of camera traps positioned at burrow entrances for determining the number of breeding pairs in a sample to inform population estimates, and for recording breeding success. Linear Discriminant Analyses of time series activity patterns from camera traps successfully partitioned breeding and non-breeding burrows at different stages of the breeding season and had reasonable predictive ability to determine breeding status on a small test dataset. Compared with traditional techniques for determining burrow occupancy (e.g. manual burrow inspection and playback of conspecific calls at burrow entrances), camera traps can reduce uncertainty in estimated breeding success and potentially breeding status of burrows. Significant up-front investment is required in terms of equipment and human resources but for long-term studies camera traps can deliver advantages, particularly when unanticipated novel observations and the potential for calibrating traditional methods with cameras are factored in.

Maximising survey efficiency can help reduce the trade-off between spending limited conservation resources on evaluating performance of past interventions and directing those resources towards future interventions. Seabird responses to island eradications are often poorly evaluated owing to financial, logistical and methodological challenges associated with remote field work and species ecology. We surveyed an assemblage of threatened seabirds following the world’s largest island eradication of multiple invasive species, testing multiple survey designs and outputs. We compared the outcomes of two important choices made during survey design: 1) whether to use unbiased or targeted surveys; and 2) implementing design-based or model-based analyses. An unbiased whole-island stratified randomised survey design performed well in terms of confidence in the final population estimates for widespread species, but poorly for localised recolonising species. For widespread species, model-based analyses resulted in slightly lower population estimates with narrower confidence intervals than traditional design-based approaches but failed to capture the realised niches of recolonising species, resulting in population estimates three orders of magnitude higher than current best estimates. We conclude that a multi-method approach to survey design best captures the size and distribution of recovering populations when the study system is ecologically diverse—importantly our results suggest there is no single strategy for efficient surveys of diverse seabird communities following large island invasive species eradications.

Population estimates are commonly generated and used in conservation science. All estimates carry inherent uncertainty, but little attention has been given to when and how this uncertainty limits their use. This requires an understanding of the specific purposes for which population estimates are intended, an assessment of the level of uncertainty each purpose can tolerate, and information on current uncertainty.We conducted a review and meta-analysis for a widespread group of seabirds, the petrels, to better understand how and why population estimates are being used. Globally petrels are highly threatened, and aspects of their ecology make them difficult to survey, introducing high levels of uncertainty into population estimates.We found that by far the most common intended use of population estimates was to inform status and trend assessments, while less common uses were trialling methods to improve estimates, and assessing threat impacts and conservation outcomes.The mean coefficient of variation for published estimates was 0.17 (SD = 0.14), with no evidence that uncertainty has been reduced through time. As a consequence of this high uncertainty, when we simulated declines equivalent to thresholds commonly used to trigger management, only 5% of studies could detect significant differences between population estimates collected 10 years apart for populations declining at a rate of 30% over three generations.Reporting of uncertainty was variable with no dispersion statistics reported with 38% of population estimates and most not reporting key underlying parameters: nest numbers/density and nest occupancy. We also found no correlation between population estimates and either island size, body size or species threat status—potential predictors of uncertainty.Synthesis and applications—Key recommendations for managers are to be mindful of uncertainty in past population estimates if aiming to collect contemporary estimates for comparison, to report uncertainty clearly for new estimates, and to give careful consideration to whether a proposed estimate is likely to achieve the requisite level of certainty for the investment in its generation to be warranted. We recommend a practitioner-based Value of Information assessment to confirm where there is value in reducing uncertainty.

Quantitative methods for taking sediment, soil, and litter samples, together with the different approaches and apparatus to extract the animals, are described. Mechanical methods discussed include wet and dry sieving, flotation, and elutriation. Behavioural or dynamic methods such as Berlese and Baermann funnels are described. Chemical and electrical methods to drive animals from cover are described, and the efficiency of the different extraction techniques is reviewed.

Methods for quantitative sampling of open marine and freshwaters are described, including fishing nets, plankton nets, pump samplers, the Patalas–Schindler volume sampler, and specialized methods for freshwater insects. Specialist methods for sampling freshwater floating, emergent, and submerged vegetation are described. Methods for quantitatively sampling the bottom fauna in the various substrates found in streams, rivers, lakes, and the sea bed are reviewed. The use of various poisons and anaesthetics for sampling fish are described.

The main methods used to estimate population size using capture–recapture for both closed and open populations are described, including the Peterson–Lincoln estimator, the Schabel census, Bailey’s triple catch, the Jolly–Seber stochastic method, and Cormack’s log-linear method. The robust design approach is described. R code listings for commonly used packages are presented. The assumptions common to capture–recapture methods are reviewed, and tests for assumptions such as equal catchability described. The use of programs to select model assumptions are described. The main methods for marking different animal groups are described, together with the use of natural marks and parasites and DNA. Marking methods include paint marks, dyes, tagging, protein marking, DNA, natural marks, tattooing, and mutilation. Methods for handling and release are described.

Methodologies based on counting the number of sightings to estimate are described. These techniques are particularly useful for large or easily seen animals such as birds, large grassland mammals, whales, crocodilians, and large, active insects such as butterflies. Point and line survey methods are described. Distance sampling methods, including Fourier series estimators, are presented, and R code listings to undertake the computations presented. Plotless density estimators are described based on nearest-neighbour, and closest-individual measurements are described.

Suction and other samplers for aerial insects and other invertebrates, such as the exposed-cone Johnson–Taylor trap, are described, and their efficiencies under different meteorological conditions defined. Techniques for sampling small animals from plants are described, including the use of suction apparatus such as the D-Vac and Vectis for grassland sampling. Specialized methods for counting insects within plant tissues are described, and techniques for sampling parasites from their vertebrate hosts and homes are reviewed.

OBJECTIVES: Persons experiencing homelessness (PEH) are disproportionately affected by tuberculosis (TB). We estimate area-specific rates of TB among PEH and characterize the extent to which available data support recent transmission as an explanation of high TB incidence. METHODS: We estimated TB incidence among PEH using National Tuberculosis Surveillance System data and population estimates for the US Department of Housing and Urban Development’s Continuums of Care areas. For areas with TB incidence higher than the national average among PEH, we estimated recent transmission using genotyping and a plausible source-case method. For cases with ≥ 1 plausible source case, we assessed with TB program partners whether available whole-genome sequencing and local epidemiologic data were consistent with recent transmission. RESULTS: During 2011–2016, 3164 TB patients reported experiencing homelessness. National incidence was 36 cases/100,000 PEH. Incidence estimates varied among 21 areas with ≥ 10,000 PEH (9–150 cases/100,000 PEH); 9 areas had higher than average incidence. Of the 2349 cases with Mycobacterium tuberculosis genotyping results, 874 (37%) had ≥ 1 plausible source identified. In the 9 areas, 23%–82% of cases had ≥ 1 plausible source. Of cases with ≥ 1 plausible source, 63% were consistent and 7% were inconsistent with recent transmission; 29% were inconclusive. CONCLUSIONS: Disparities in TB incidence for PEH persist; estimates of TB incidence and recent transmission vary by area. With a better understanding of the TB risk among PEH in their jurisdictions and the role of recent transmission as a driver, programs can make more informed decisions about prioritizing TB prevention strategies.

Objectives-This report presents complete period life tables for each of the 50 states and the District of Columbia by sex based on age-specific death rates in 2018. Methods-Data used to prepare the 2018 state-specific life tables include 2018 final mortality statistics; July 1, 2018 population estimates based on the 2010 decennial census; and 2018 Medicare data for persons aged 66-99. The methodology used to estimate the state-specific life tables is the same as that used to estimate the 2018 national life tables, with some modifications. Results-Among the 50 states and the District of Columbia, Hawaii had the highest life expectancy at birth, 81.0 years in 2018, and West Virginia had the lowest, 74.4 years. Life expectancy at age 65 ranged from 17.5 years in Kentucky to 21.1 years in Hawaii. Life expectancy at birth was higher for females in all states and the District of Columbia. The difference in life expectancy between females and males ranged from 3.8 years in Utah to 6.2 years in New Mexico.

Pneumonia constitutes a substantial disease burden among adults overall and those who are elderly. We aimed to identify all studies investigating the disease burden among older adults (age, ≥65 years) admitted to the hospital with pneumonia. We estimated the hospital admission rate and in-hospital case-fatality ratio (CFR) of pneumonia in older adults, stratified by age and economic status (industrialized vs developing), with data from a systematic review of studies published from 1996 through 2017 and from 8 unpublished population-based studies. We applied these rate estimates to population estimates for 2015 to calculate the global and regional burden in older adults who would have been admitted to the hospital with pneumonia that year. We estimated the number of in-hospital pneumonia deaths by combining in-hospital CFRs with hospital admission estimates from hospital-based studies. We identified 109 eligible studies; 73 used clinical pneumonia as the case definition, and 36 used radiologically confirmed pneumonia as the case definition. We estimated that, in 2015, 6.8 million episodes (uncertainty range [UR], 5.8–8.0 episodes) of clinical pneumonia resulted in hospital admissions of older adults worldwide. The hospital admission rate increased with advancing age and was higher in men. The total disease burden was likely underestimated when using the definition of radiologically confirmed pneumonia. Based on data from 52 hospital studies reporting data on pneumonia mortality, we estimated that about 1.1 million in-hospital deaths (UR, 0.9–1.4 in-hospital deaths) occurred among older adults. The burden of pneumonia requiring hospitalization among older adults is substantial. Appropriate prevention and management strategies should be developed to reduce its impact.

This paper will critically assess the utility of conventional and novel data sources for building fine-scale spatio-temporal estimates of the ambient population. It begins with a review of data sources employed in existing studies of the ambient population, followed by preliminary analysis to further explore the utility of each dataset. The identification and critiquing of data sources which may be useful for building estimates of the ambient population are novel contributions to the literature. This paper will provide a framework of reference for researchers within urban analytics and other areas where an accurate measurement of the ambient population is required. This work has implications for national and international applications where accurate small area estimates of the ambient population are crucial in the planning and management of urban areas, the development of realistic models and informing policy. This research highlights workday population estimates, in conjunction with footfall camera and Wi-Fi sensors data as potentially valuable for building estimates of the ambient population.

Published analysis of genetic material from field-collected tsetse (Glossina spp, primarily from the Palpalis group) has been used to predict that the distance (δ) dispersed per generation increases as effective population densities (De) decrease, displaying negative density-dependent dispersal (NDDD). Using the published data we show this result is an artefact arising primarily from errors in estimates of S, the area occupied by a subpopulation, and thereby in De. The errors arise from the assumption that S can be estimated as the area (S^) regarded as being covered by traps. We use modelling to show that such errors result in anomalously high correlations between δ^ and S^ and the appearance of NDDD, with a slope of -0.5 for the regressions of log(δ^) on log(D^e), even in simulations where we specifically assume density-independent dispersal (DID). A complementary mathematical analysis confirms our findings. Modelling of field results shows, similarly, that the false signal of NDDD can be produced by varying trap deployment patterns. Errors in the estimates of δ in the published analysis were magnified because variation in estimates of S were greater than for all other variables measured, and accounted for the greatest proportion of variation in δ^. Errors in census population estimates result from an erroneous understanding of the relationship between trap placement and expected tsetse catch, exacerbated through failure to adjust for variations in trapping intensity, trap performance, and in capture probabilities between geographical situations and between tsetse species. Claims of support in the literature for NDDD are spurious. There is no suggested explanation for how NDDD might have evolved. We reject the NDDD hypothesis and caution that the idea should not be allowed to influence policy on tsetse and trypanosomiasis control., Author summary Published analysis of genetic material from field-sampled tsetse (Glossina spp) has been used to suggest that, as tsetse population densities decrease, rates of dispersal increase–displaying negative density-dependent dispersal (NDDD), perhaps in all tsetse species. It is further suggested that tsetse control operations might, as a consequence of NDDD, unleash enhanced invasion of areas cleared of tsetse, prejudicing the long-term success of control campaigns. We demonstrate that NDDD in tsetse is an artefact consequent on multiple errors of analysis and interpretation. The most serious of these errors stems from a misunderstanding of the way in which traps sample tsetse, resulting in large errors in estimates of the areas covered by the traps, and occupied by the subpopulations being sampled. Our modelling studies show that these errors can produce the false signal of NDDD, even in situations where DID is assumed. Errors in census population estimates are made worse through failure to adjust for variations in trapping intensity, trap performance, and in capture probabilities between geographical situations, and between tsetse species. We reject the NDDD hypothesis and caution that the idea should not be allowed to influence policy on tsetse and trypanosomiasis control.