Search

Due to increasing numbers of anthropogenic chemicals with unknown neurotoxic properties, there is an increasing need for a paradigm shift toward rapid and higher throughput behavioral bioassays. In this work, we demonstrate application of a purpose-built high throughput multidimensional behavioral test battery on larval stages of Danio rerio (zebrafish) at 5 days post fertilization (dpf). The automated battery comprised of the established spontaneous swimming (SS), simulated predator response (SPR), larval photomotor response (LPR) assays as well as a new thermotaxis (TX) assay. We applied the novel system to characterize environmentally relevant concentrations of emerging pharmaceutical micropollutants including anticonvulsants (gabapentin: 400 ng/L; carbamazepine: 3000 ng/L), inflammatory drugs (ibuprofen: 9800 ng/L), and antidepressants (fluoxetine: 300 ng/L; venlafaxine: 2200 ng/L). The successful integration of the thermal preference assay into a multidimensional behavioral test battery provided means to reveal ibuprofen-induced perturbations of thermal preference behaviors upon exposure during embryogenesis. Moreover, we discovered that photomotor responses in larval stages of fish are also altered by the as yet understudied anticonvulsant gabapentin. Collectively our results demonstrate the utility of high-throughput multidimensional behavioral ecotoxicity test batteries in prioritizing emerging risks associated with neuroactive drugs that can perturb neurodevelopment. Moreover, we showcase the added value of thermotaxis bioassays for preliminary screening of emerging contaminants. [ABSTRACT FROM AUTHOR]

Vertebrates show substantial interspecific variation in brain size in relation to body mass. It has long been recognized that the evolution of large brains is associated with both costs and benefits, and it is their net benefit which should be favoured by natural selection. On one hand, the substantial energetic cost imposed by the maintenance of neural tissue is expected to compromise the energetic budget of organisms with large brains and their investment in other critical organs (expensive brain framework, EBF) or important physiological process, such as somatic maintenance and repair, thus accelerating ageing that shortens lifespan, as predicted by the disposable soma theory (DST). However, selection towards larger brain size can provide cognitive benefits (e.g., high behavioural flexibility) that may mitigate extrinsic mortality pressures, and thus may indirectly select for slower ageing that prolongs lifespan, as predicted by the cognitive buffer hypothesis (CBH). The relationship between longevity and brain size has been investigated to date only among terrestrial vertebrates, although the same selective forces acting on those species may also affect vertebrates living in aquatic habitats, such as fish. Thus, whether this evolutionary trade‐off for brain size and longevity exists on a large scale among fish clades remains to be addressed. In this study, using a global dataset of 407 fish species, I undertook the first phylogenetic test of the brain size/longevity relationship in aquatic vertebrate species. The study revealed a negative relationship between brain size and longevity among cartilaginous fish confirming EBF and DST. However, no pattern emerged among bony fish species. Among sharks and rays, the high metabolic cost of producing neural tissue transcends the cognitive benefits of evolving a larger brain. Consequently, my findings suggest that the cost of maintaining brain tissue is relatively higher in ectothermic species than in endothermic ones. [ABSTRACT FROM AUTHOR]

It is a topic that has not been sufficiently researched, thus requiring investigations into the results, that can be had if we apply water-based exercises. In this sense, we conducted a study, during 10 weeks, which consisted in developing and experimenting with muscle training programs in the aquatic environment and on land, as well as testing two strength indicators on a number of 24 students, aged 20-21 years, who were selected voluntarily and divided into 2 experimental groups in order to compare the results of the development of muscle strength of the upper body. The same training and testing facilities, methods, were used for each assessment and lesson. Each lesson lasted between 60 - 80 minutes, 3 days a week. The subjects improved in strength scores significantly, as compared to the control group, which followed the same muscular training protocol adapted for land, having a value comprised between: 1, 25 and 7, 98%. Subiectul nu a fost suficient cercetat, necesitând astfel investigații asupra rezultatelor, care poate fi dacă aplicăm în pregătirea musculară exerciții în mediul acvatic. În acest sens, am realizat un studiu, pe parcursul a 10 săptămâni, care a constat în elaborarea și experimentarea programelor de antrenament muscular în mediul acvatic și pe uscat, precum și în testarea a doi indicatori de forță pe un număr de 24 de elevi, cu vârsta cuprinsă între 20-21 de ani. Ei au fost selectați voluntar și împărțiți în 2 grupuri experimentale pentru a compara rezultatele dezvoltării forței musculare a corpului superior. Au fost folosite aceleași facilități de instruire și testare, metode, pentru fiecare evaluare și lecție. Fiecare lecție a durat între 60 - 80 de minute, 3 zile pe săptămână. Subiecții și-au îmbunătățit semnificativ în scorurile de forță, comparativ cu grupul de control, care a urmat același protocol de antrenament adaptat pe sol, având o valoare cuprinsă între: 1, 25 și 7, 98%. [ABSTRACT FROM AUTHOR]

Astaxanthin is a carotenoid with antioxidant properties due to its special chemical structure. Biosynthesis and chemical synthesis are the most commonly used methods for astaxanthin synthesis, natural astaxanthin can be extracted from yeast and algae. In aquatic animals, β -carotene can be converted into astaxanthin through multiple metabolic processes. At present, natural astaxanthin and synthetic astaxanthin have been widely used in practical production and life, such as medicine, food, animal husbandry and other fields. The application of astaxanthin in aquatic animal bait can improve immunity and growth performance and improve body color. The article reviews the production, synthesis and biological metabolism of astaxanthin, the distribution of organisms and its application value in production, to provide references for related research and applications. [ABSTRACT FROM AUTHOR]

The species composition of aquatic (Nepomorpha) and semiaquatic (Gerromorpha) Heteroptera were examined from protected and unprotected study sites in three streams associated with Kaeng Krachan National Park. At each stream, both quantitative and qualitative sampling methods were used during seven collecting events (November 2018 to June 2020). A total of 11 families, representing 33 genera and 60 species, were collected in this study, with more Nepomorpha families but higher species richness in Gerromorpha. The species richness of both protected and unprotected sampling sites were lowest during the fifth sampling event. Nevertheless, there was no significant difference in richness between protected and unprotected sampling sites for any sampling event based on a paired t-test analysis. Based on an nMDS analysis, the patterns of species composition of aquatic and semiaquatic heteropterans were unclear among protected and unprotected sampling sites. The use of aquatic and semiaquatic Heteroptera as bioindicators for habitat quality is still uncertain. Additional physiochemical characters of the water and physical characters of the stream may lead to a clearer picture of the relationship between aquatic and semiaquatic Heteroptera and stream habitat quality. [ABSTRACT FROM AUTHOR]

Non-parasitic flatworms are known to temporarily attach to the substrate by secreting a multicomponent bioadhesive to counteract water movements. However, to date, only species of two higher-level flatworm taxa (Macrostomorpha and Proseriata) have been investigated for their adhesive proteins. Remarkably, the surface-binding protein is not conserved between flatworm taxa. In this study, we sequenced and assembled a draft genome, as well as a transcriptome, and generated a tail-specific positional RNA sequencing dataset of the polyclad Theama mediterranea. This led to the identification of 15 candidate genes potentially involved in temporary adhesion. Using in situ hybridisation and RNA interference, we determined their expression and function. Of these 15 genes, 4 are homologues of adhesion-related genes found in other flatworms. With this work, we provide two novel key components on the flatworm temporary adhesion system. First, we identified a Kringle-domain-containing protein (Tmed-krg1), which was expressed exclusively in the anchor cell. This in silico predicted membrane-bound Tmed-krg1 could potentially bind to the cohesive protein, and a knockdown led to a non-adhesive phenotype. Secondly, a secreted tyrosinase (Tmed-tyr1) was identified, which might crosslink the adhesive proteins. Overall, our findings will contribute to the future development of reversible synthetic glues with desirable properties for medical and industrial applications. [ABSTRACT FROM AUTHOR]

Remote sensing approaches that could identify species of submerged aquatic vegetation (SAV) and measure their extent in lake littoral zones would greatly enhance SAV study and management, especially if these approaches can provide faster or more accurate results than traditional field methods. Remote sensing with multispectral sensors can provide this capability, but SAV identification with this technology must address the challenges of light extinction in aquatic environments where chlorophyll, dissolved organic carbon, and suspended minerals can affect water clarity and the strength of the sensed light signal. Here, we present an uncrewed aerial system (UAS)-enabled methodology to identify the extent of the invasive SAV species Myriophyllum spicatum (Eurasian watermilfoil, or EWM), primarily using a six-band Tetracam multispectral camera, flown over sites in the Les Cheneaux Islands area of northwestern Lake Huron, Michigan, USA. We analyzed water chemistry and light data and found our sites clustered into sites with higher and lower water clarity, although all sites had relatively high water clarity. The overall average accuracy achieved was 76.7%, with 78.7% producer's and 77.6% user's accuracy for the EWM. These accuracies were higher than previously reported from other studies that used remote sensing to map SAV. Our study found that two tested scale parameters did not lead to significantly different classification accuracies between sites with higher and lower water clarity. The EWM classification methodology described here should be applicable to other SAV species, especially if they have growth patterns that lead to high amounts of biomass relative to other species in the upper water column, which can be detected with the type of red-edge and infrared sensors deployed for this study. [ABSTRACT FROM AUTHOR]

Background: Aquatic physiotherapy is becoming a more frequently utilised treatment for people with Parkinson's Disease (PD). Consumers are increasingly accessing information regarding health choices online, and it is not known what type or quality of information regarding aquatic physiotherapy is available.Methods: Web-based platforms (Facebook™, Twitter™, YouTube™, Instagram™, blogs and the web) were searched using the Awario© social listening software. Webpages had to be in English, mention PD, aquatic physiotherapy and its effects. Quality of webpages was assessed using a modified DISCERN tool and content analysis summarised reported effects.Results: Awario© identified 2992 entries, with 133 assessed using the modified DISCERN tool. A small number (n = 31, 24%) described the effects of aquatic physiotherapy for people with PD. Quality of webpages was low, with many lacking information regarding clear sources of information, contraindications to aquatic physiotherapy and descriptions of the therapeutic environment. Content analysis showed several themes; general physical, PD-specific and psychosocial effects. More than a third of webpages indicated that aquatic physiotherapy would improve strength, balance, pain and aid relaxation. A large number (n = 96, 72%) described at least one hydrodynamic or hydrostatic property of water, most commonly buoyancy (n-83, 62%).Conclusions: Overall quality of information was poor, and it is recommended that webpages list all potential contraindications to aquatic physiotherapy and direct consumers to discuss potential participation with their healthcare professionals. Webpages also should include information regarding the therapeutic environment, disclose sources of information and focus on enablers to exercise to improve engagement of people with PD in aquatic physiotherapy. [ABSTRACT FROM AUTHOR]

Since 2011, we have collected fungi that form synnema-like structures (SSs) bearing many acanthophyses at the apex on water-splashed wood in streams in various regions of Japan. A provisional phylogenetic analysis of strains isolated from SSs based on their nrDNA sequences implied affinity with Physisporinus (Polyporales, Basidiomycota). However, it has not been reported that this genus forms SSs in freshwater habitats. We found a fungus forming not only SSs on the water-boundary part of wood but also resupinate basidiocarps with poroid hymenophores on nonsubmerged parts, and the morphological characteristics of the basidiocarps matched those of Physisporinus. Therefore, we investigated the relationship between SS-forming fungi and their sexual states by taxonomic approaches. Phylogenetic analyses based on nrDNA internal transcribed spacer (ITS) and large subunit (LSU) sequences indicated that SS-forming fungi diverged into five clades in Physisporinus. Each clade was discriminated by the color of SSs, morphology of acanthophyses, and cultural characteristics. Of the five clades, Clade 1, which consisted only of sequences of strains isolated from SSs and basidiocarps produced on rhizomorphs, was closely related to P. eminens and P. undatus, but the morphology of basidiocarps and the manner of basidiocarp development differed. Clade 5 was closely related to P. castanopsidis, P. crocatus, P. pouzarii, P. sanguinolentus, P. subcrocatus, P. tibeticus, and P. vitreus, but the basidiocarp morphology differed. Therefore, Clades 1 and 5 were described as two new species. Regarding Clades 2, 3, and 4, further taxonomic studies with additional specimens are required. SS and acanthophysis formation in wet habitats in streams and in culture could be recognized as new taxonomic and ecological characters of Physisporinus. [ABSTRACT FROM AUTHOR]

Aim: Abundance–occupancy relationships posit that more locally abundant species occupy more sites than less abundant species. Although widely supported, the occurrence and detection of abundance–occupancy relationships is sensitive to sampling and detection processes. Data from large‐scale standardized sampling efforts are key to address abundance–occupancy relationships. We aimed to use such a dataset to evaluate the occurrence of abundance–occupancy relationships across different spatial grains and over time for aquatic and terrestrial taxa. Location: USA. Time period: 2014–2019. Major taxa studied: Birds, mammals, beetles, ticks, fishes, macroinvertebrates and zooplankton. Methods: Species abundance and occupancy data were obtained from the National Ecological Observatory Network (NEON). Species mean abundance and occupancy (fraction of sampled locations that were occupied) were estimated for three different spatial grains (i.e., plot, site and domain) for all years sampled. Linear models were used to explore the consistency of interspecific abundance–occupancy relationships. The slope coefficients of these models were related to temporal and spatial variables and to species richness while controlling for taxa in a linear mixed‐effects model (LMM) framework. Results: We found evidence for positive abundance–occupancy relationships across the three spatial grains and over time for all taxa we studied. However, our linear models had low explanatory power, suggesting that relationships, although general, were weak. Abundance–occupancy relationships were slightly stronger at the smallest spatial grain than at the largest spatial grain, but showed no detectable change over time for any taxa. Finally, species richness was not associated with the strength of these relationships. Main conclusions: Together, our results suggest that positive interspecific abundance–occupancy relationships are fairly general but are not capable of explaining substantial variation in spatial patterns of abundance, and that other factors, such as species traits and niche, are also likely to influence these relationships. [ABSTRACT FROM AUTHOR]

Microplastics are plastic fragments with a size less than 5 mm in length. In addition to a threat to the marine environments where these are abundantly present, these have also started polluting freshwater ecosystems. However, the uptake of microplastics by living organisms differs depending on their habitats and feeding behaviors. We investigated the presence, size, type, and color of microplastics in the gastrointestinal tract contents of two water snakes, namely Natrix natrix and Natrix tessellata. The snakes were collected from different regions of Turkey and preserved as museum materials. Our results showed that fibers constituted the predominant polymer type in both snake species (94.7% for N. natrix and 87.9% for N. tessellata), whose dimensions ranged from 250 to 3750 µm. We did not find any significant difference in the number of microplastics ingested between the two species. In addition, the uptake of microplastics did not relate to the size and weight of snakes. Microplastics were not consistently present all years, and similarly, these were not detected in all samples within the distribution area. These results could be attributed to the environment and diet of two snake species. [ABSTRACT FROM AUTHOR]

A modeling framework was created for the development of spatially explicit aquatic exposure models for any region or country of interest for chemicals disposed of down the drain. The framework relies on globally available data sets for river flow and population, and locally available data sets for wastewater treatment infrastructure and domestic water use, and leverages the iSTREEM® chemical routing algorithm. The framework was applied to China and Japan as case study countries. Spatially explicit population data were obtained from WorldPop. River flows covering the spatial extent of the two countries were derived from a high‐resolution surface runoff gridded data set that was based on the Curve Number approach and combined with the hydrology network for catchments and rivers from HydroBASINS and HydroSHEDS data sets. Publicly available data from government sources were used for estimating per capita water use and wastewater treatment infrastructure. To demonstrate the framework, the China model was used to predict the levels of the antifungal agent climbazole in rivers across the country, and the Japan model was used to predict river concentrations of linear alkylbenzene sulfonate. For both chemicals, the comparison of measured to modeled values showed good agreement, using linear regression analysis (R2 ≥ 0.96). The framework presented in this study provides a systematic and robust approach to develop spatially resolved exposure models that can be extrapolated to any country or region, allowing more accurate risk assessment of chemicals disposed down the drain by leveraging concentration distributions generated by the model. Integr Environ Assess Manag 2022;18:722–733. © 2021 SETAC Key Points: Modeling framework created for the development of spatially explicit aquatic exposure models for any region or country of interest for chemicals disposed of down the drain.Framework relies on the use of a high‐resolution global flow dataset developed as part of this research.Framework also relies on globally available datasets for population and locally available datasets for wastewater treatment infrastructure and domestic water use.Model framework was successfully applied to two case study countries, China and Japan, which differ significantly in population density, river flow (due to climate and geography), and wastewater treatment infrastructure. [ABSTRACT FROM AUTHOR]

Freshwater ecosystems and fishes are enormous resources for human uses and biodiversity worldwide. However, anthropogenic climate change and factors such as dams and environmental contaminants threaten these freshwater systems. One way that researchers can address conservation issues in freshwater fishes is via integrative non-lethal movement research. We review different methods for studying movement, such as with acoustic telemetry. Methods for connecting movement and physiology are then reviewed, by using non-lethal tissue biopsies to assay environmental contaminants, isotope composition, protein metabolism, and gene expression. Methods for connecting movement and genetics are reviewed as well, such as by using population genetics or quantitative genetics and genome-wide association studies. We present further considerations for collecting molecular data, the ethical foundations of non-lethal sampling, integrative approaches to research, and management decisions. Ultimately, we argue that non-lethal sampling is effective for conducting integrative, movement-oriented research in freshwater fishes. This research has the potential for addressing critical issues in freshwater systems in the future. [ABSTRACT FROM AUTHOR]

Duckweed (Lemna minor L.) is an aquatic macrophyte and grows profoundly on the surface of polluted water reservoirs of Pakistan. The plant can be used as a potential alternative for the fish and poultry industry to meet the promptly growing demand for feed. Our study investigates the effect of varying concentrations (ppm) of nutrients like N, P, and their combination, NP on biomass production, carbohydrate, lipid, protein, and mineral (Ca, Mg, Fe, Mn & Zn) contents of L. minor. The varying concentrations of N and P substantially affected the above-stated parameters. The highest biomass yield was recorded in the 30 ppm NP tank as 172 g/m2 day in comparison with the control tank. Higher protein, lipid, and carbohydrate contents were recorded for 30 ppm NP, 20 ppm NP, and 10 ppm NP respectively. Minerals like Ca, Mg, Fe, Mn & Zn increased in 20 ppm P and all N concentrations. The combined application of NP was more effective in boosting the protein, carbohydrate, and lipid content whereas less effective in increasing the mineral contents. A rise in the concentration of N and P showed a positive correlation with the nutritional composition of L. minor. [ABSTRACT FROM AUTHOR]

The genus Paracladopus was established based on the type species P. chiangmaiensis in 2006. The two Paracladopus species are distributed in Thailand and Laos; however, neither of them has been documented in China to date. During our field work in 2020, we collected a river-weed in Wuzhi Mountain, Hainan Province of China. After checking the morphological characters, it was identified as P. chiangmaiensis. Then, we assembled and annotated its chloroplast genome based on the genome skimming data. The results showed that the complete chloroplast genome was 133,748 bp with 35% GC content, consisting of 76 protein-coding genes, 30 tRNA genes, and 4 rRNA genes. A maximum-likelihood tree constructed based on the matk genes showed that WuMS109 was clustered with P. chiangmaiensis (AB537420, AB698348) without base difference and together with the remains of Paracladopus formed a sister clade to Cladopus. This is the first report of P. chiangmaiensis that represents a new generic record for China. The discovery of this river-weed could lay the foundation for investigating their biogeographical patterns and species evolution in further studies. [ABSTRACT FROM AUTHOR]

Biological dinitrogen (N2) fixation is performed solely by specialized bacteria and archaea termed diazotrophs, introducing new reactive nitrogen into aquatic environments. Conventionally, phototrophic cyanobacteria are considered the major diazotrophs in aquatic environments. However, accumulating evidence indicates that diverse non-cyanobacterial diazotrophs (NCDs) inhabit a wide range of aquatic ecosystems, including temperate and polar latitudes, coastal environments and the deep ocean. NCDs are thus suspected to impact global nitrogen cycling decisively, yet their ecological and quantitative importance remain unknown. Here we review recent molecular and biogeochemical evidence demonstrating that pelagic NCDs inhabit and thrive especially on aggregates in diverse aquatic ecosystems. Aggregates are characterized by reduced-oxygen microzones, high C:N ratio (above Redfield) and high availability of labile carbon as compared to the ambient water. We argue that planktonic aggregates are important loci for energetically-expensive N2 fixation by NCDs and propose a conceptual framework for aggregate-associated N2 fixation. Future studies on aggregate-associated diazotrophy, using novel methodological approaches, are encouraged to address the ecological relevance of NCDs for nitrogen cycling in aquatic environments. [ABSTRACT FROM AUTHOR]

The larva of Corethrella kipferi Dorff, Borkent and Curler sp. nov. occupies hyporheic habitat regardless of perennial surface flow in two close locales in the Ozark Mountains in Christian County, Missouri, USA. This represents the first capture and identification to species level of Corethrella in the hyporheic zone and the only description of a larva from the rotunda species group. The larvae appear to be obligate hyporheic dwellers, with no representatives collected in concurrent benthic samples from riffles of the perennial stream. The female adult and fourth-instar larva are described and illustrated using photomicrographs and line drawings. We describe what is known of the natural history of the new species and infer its phylogenetic placement within the genus. [ABSTRACT FROM AUTHOR]

A total of 5183 adult aquatic insects, representing 3 orders, 7 families, and 16 genera, and 1447 adult terrestrial insects representing 8 orders and 21 families, were light trapped at Lake Murray Reservoir, San Diego County, California, from 17 April-25 November 2019. Diptera was the most abundant taxon for all sampled individuals. A single species of mayfly, Caenis amica Hagen, 1861, was collected. Five aquatic Diptera and three Trichoptera species are new distribution records for San Diego County. A second state distribution record for the micro-caddisfly, Oxyethira arizona Ross, 1948, is also reported. New observations of adult swarming behavior of the caddisfly, Oecetis inconspicua (Walker, 1852), are reported. [ABSTRACT FROM AUTHOR]

The presence of edible and inedible prey species in a food web can influence the strength that nutrients (bottom‐up) or herbivores (top‐down) have on primary production. In boreal peatlands, wetter more nutrient‐rich conditions associated with ongoing climate change are expanding consumer access to aquatic habitat and promoting sources of primary production (i.e., algae) that are susceptible to trophic regulation. Here, we used an in situ mesocosm experiment to evaluate the consequences of enhanced nutrient availability and food‐web manipulation (herbivore and predator exclusion) on algal assemblage structure in an Alaskan fen. Owing to the potential for herbivores to selectively consume edible algae (small cells) in favor of more resistant forms, we predicted that the proportion of less‐edible algae (large cells) would determine the strength of top‐down or bottom‐up effects. Consistent with these expectations, we observed an increase in algal‐cell size in the presence of herbivores (2‐tiered food web) that was absent in the presence of a trophic cascade (3‐tiered food web), suggesting that predators indirectly prevented morphological changes in the algal assemblage by limiting herbivory. Increases in algal‐cell size with herbivory were driven by a greater proportion of filamentous green algae and nitrogen‐fixing cyanobacteria, whose size and morphological characteristics mechanically minimize consumption. While consumer‐driven shifts in algal assemblage structure were significant, they did not prevent top‐down regulation of biofilm development by herbivores. Our findings show that increasing wet periods in northern peatlands will provide new avenues for trophic regulation of algal production, including directly through consumption and indirectly via a trophic cascade. [ABSTRACT FROM AUTHOR]

The active chemicals in sunscreen formulations are released into the environment from human skin, and found in recreational-use waters like seawater, but can also be washed into fresh water from bathing and showering. The level of sunscreen chemicals found in samples varies considerably between regions, time of year (higher in summer months), and time of day. Average typical concentrations are only in the nanograms per litre (ng L−1) range in marine and fresh water systems, and typically, the highest levels are in waste-water sludge because of a concentrating effect during the treatment process. From numerous studies, it is known that the active chemicals in sunscreens can have potential hormonal/oestrogenic activity and non-hormonal effects, including: acting as teratogens, altering gene regulation, inducing changes in antioxidant and free radical production, and inducing coral bleaching. However, the effects of sunscreens on aquatic life under laboratory conditions typically occur only at concentrations (µg or mg L−1) that far exceed (10–10 000-fold) levels found in the environment. As such, when damage does occur to reefs and animal life, there are often other causes that are more likely impacting the aquatic life including changes in water temperature, water turbidity, elevated nutrient levels, and the presence of pesticides and medicines used for human and animal health. While the chemicals in sunscreen formulations can be found in salt and fresh water systems, and they are able to damage plant and animals under laboratory conditions, they are only found at very low levels in the environment. [ABSTRACT FROM AUTHOR]

Open‐water perennial pools are common natural features of peatlands globally, and peatland restoration often results in new pool creation, yet the concentrations of different forms of aquatic carbon (C) in natural and artificial restoration pools are not well studied. We compared carbon concentrations in both natural pools and restoration pools (4–15 years old) on three blanket peatlands in northern Scotland. At all sites, restoration pools were more acidic and had mean dissolved organic carbon (DOC) concentrations in restoration pools of 23, 22, and 31 mg L−1 compared with natural pool means of 11, 11 and 15 mg L−1 respectively across the three sites. Restoration pools had a greater fulvic acid prevalence than the natural pools and their DOC was more aromatic. Restoration pools were supersaturated with dissolved CO2 at around 10 times atmospheric levels, whereas for natural pools, CO2 concentrations were just above atmospheric levels. Dissolved CH4 concentrations were not different between pool types, but were ~200 times higher than atmospheric levels. Regular sampling at one of the peatland sites over 2.5 years showed that particulate organic carbon (POC) concentrations were generally below 7 mg L−1 except during the warm, dry summer of 2013. At this regularly‐sampled site, natural pools were found to process DOC so that mean pool outflow concentrations in overland flow were significantly lower than mean inflow DOC concentrations. Such an effect was not found for the restoration pools. Soil solution and pool water chemistry, and relationships between DOC and CO2 concentrations suggest that different processes are controlling the transformation of C, and therefore the form and amount of C, in natural pools compared to restoration pools. [ABSTRACT FROM AUTHOR]

Background: Climate is one of the most important driving factors of future changes in terrestrial, coastal, and marine ecosystems. Any changes in these environments can significantly influence physiological and behavioural responses in aquatic animals, such as crustacea. Crustacea play an integral role as subsistence predators, prey, or debris feeders in complex food chains, and are often referred to as good indicators of polluted or stressed conditions. They also frequently have high production, consumption, and commercial significance. However, crustacean's responses to climate change are likely to vary by species, life-history stage, reproduction status and geographical distribution. This map is undertaken as part of the Long-Term Research Grant project which aims to identify any interactive effect on physiological compensation and behavioural strategy of how marine organisms, especially crustaceans, deal with stress from environmental change. Our proposed map will aim to outline the evidence currently existing for the impacts of climate change on the physiology and behaviour of important aquaculture crustacean species within Asia. Methods: We will document peer-reviewed articles in English using published journal articles and grey literature. Two bibliographic databases (Scopus and Web of Science) and multiple organizational websites with Google scholars will be searched. The systematic map protocol will follow in accordance with the Collaboration for Environmental Evidence Guidelines and Standards. Literature will be screened at the title, abstract, and full-text level using pre-defined inclusion criteria. The map will highlight marine crustacea physiological compensation and behavioural strategies to cope with climate change. It will also improve our knowledge of the available evidence and current gaps for future research recommendations. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Sport Sciences / Magallat ulum Al-Riyadat is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Microbes in snow and ice ecosystems in polar regions contribute substantially to C, N, and P cycling, but few studies have explored microbial activity in seasonal snow. The purpose of this study was to explore the relative importance of snow microbial processing of C, N, and P compounds in atmospheric deposition and litter and detect elemental limitations of snow microbes in Rocky Mountain conifer forests. Enzyme activity in snow was orders of magnitude greater than activity reported for lentic and lotic waters in similar environments. Proportions of C/P‐ and C/N‐acquiring enzymes suggest that snow samples were P limited, or C and P co‐limited, while lentic and lotic waters were more N limited. As such, microbes in seasonal snow may change the composition of nutrients and carbon, but these processes are vulnerable to changes in atmospheric deposition and snow extent and duration, which could affect nutrient processing across large areas. [ABSTRACT FROM AUTHOR]

Abstract Robust impact evaluations are needed for conservation to learn and grow as a field. Currently we lack a large body of evidence on the effects of behaviour change interventions in social‐ecological systems. By uncovering mechanistic relationships and establishing causality we can refine future programmes to enhance likelihood of effectiveness. Although a range of sophisticated methodological approaches to evaluation have been developed, conceptually linking project outcomes with conservation impacts remains difficult in complex systems. For example, sea turtles are one taxon in which unsustainable harvesting has been a particular problem. There have been a number of campaigns to reduce demand for sea turtle products, but we still have little evidence documenting their outcomes. We conducted the first formal impact evaluation of a conservation marketing campaign aimed at reducing the consumption of sea turtle meat and eggs. The campaign took place on the island of São Tomé, Central Africa, and included traditional mass media advertisements as well as community events. This is one of few demand reduction evaluations that has assessed both human behaviour and biological conservation outcomes. It benefited from an advantageous setting for evaluation, as nesting sea turtles are relatively easy to monitor and the island's small size and geographic isolation increased the detectability of potentially confounding factors. We found a decrease in self‐reported sea turtle egg consumption and a decrease in poaching of adult sea turtles. However, multiple unforeseen difficulties arose which complicated attempts at causal attribution. We were hampered by spatial spillovers, design effects from the sensitive questioning technique, concurring law enforcement and changes in biological monitoring effort. These challenges highlight the difficulties faced by practitioners seeking to apply impact evaluations in the field. We reflect on what this means for future impact evaluations of behaviour change interventions in conservation. Our recommendations include combining multiple outcome measures to triangulate hard‐to‐measure behaviours and theory‐based evaluation methods to explore causal impacts. A free Plain Language Summary can be found within the Supporting Information of this article.

Abstract Objective There is an urgent need for the discovery and/or development of novel antibiotics. We report an exploration of “slow”-growing bacteria, which can be difficult to isolate using rich media as they are usually outcompeted by “fast”-growing bacteria, as potential sources of novel antimicrobials. Results Pseudomonas sp. RIT 623 was isolated using pond water agar from a pond located on the campus of the Rochester Institute of Technology (RIT). The genome was sequenced and analyzed for potential secondary metabolite gene clusters. Bioinformatics analysis revealed 14 putative gene clusters predicted to encode pathways for the anabolism of secondary metabolites. Ethyl acetate extracts from spent growth medium of Pseudomonas sp. RIT 623 were tested against two Gram-negative (E. coli ATCC 25922 and P. aeruginosa ATCC 27853) and two Gram-positive (B. subtilis BGSC 168 and S. aureus ATCC 25923) type strains to assess antibiotic activity. The antibiotic assays demonstrated that extracts of Pseudomonas sp. RIT 623 were able to inhibit the growth of the four strains. The active compound was separated using diethyl ether in a multi-solvent extraction and reverse phase chromatography. The bioactive compound/s were subsequently eluted in two consecutive fractions corresponding to approximately 16–22% acetonitrile, indicative of polar compound/s.

BackgroundIguaçu National Park (INP) is known worldwide due to Iguaçu Waterfalls, being considered a World Natural Heritage by UNESCO. The INP is one of the last large forested extensions of inland Brazil that provides protection to the Atlantic Forest, one of the world’s biodiversity hotspots. However, its Natural Heritage status has been threatened by the construction and operation of the Baixo Iguaçu dam, agricultural and urban impacts on its boundaries and the increasing interest of the Brazilian government in re-opening of the “Colono road”, an old illegal road that crossed the interior of the park. Indeed, since benthic macroinvertebrates have been widely used for the environmental assessment of streams, records and abundance of their taxa under different seasonal periods may provide an additional dataset for biomonitoring of hydrographic systems in the face of current anthropogenic impacts on the INP boundaries and other similar streams on forest edges.New informationIn this study, we improved the sampling design of benthic macroinvertebrates and provided seasonal records covering distinct precipitation/temperature periods between 2016 and 2017 of a stream on the eastern edge of the Iguaçu National Park, Brazil. The records total 2,840 individuals distributed in 88 different taxa. The most abundant taxa were the Diptera subfamilies, Chironominae (n = 1,487) and Tanypodinae (n = 256), besides the Heterelmis genus (n = 152, Elmidae; Coleoptera). Diptera was the richest order in number of families (n = 8), while Leptophlebiidae (Ephemeroptera) was the richest taxonomic family in number of genera (n = 11). Aegla (Crustacea) and the Insecta genera, Heterelmis, Hexacylloepus, Noelmis, Phylloicus and Thraulodes, were recorded through all samplings. Twenty-five genera of Ephemeroptera, Plecoptera, Trichoptera (EPT) and Odonata were recorded during intermediate precipitation/temperature periods. Twenty-one of them were recorded in May 2016, with five genera standing out in abundance (Hydrosmilodon, Anacroneuria, Argia, Coryphaeschna, Americabaetis) and four (Needhamella, Tikuna, Simothraulopsis, Neocordulia) in December 2016. Four general taxa were exclusive of the lower precipitation/temperature period (August 2016), standing out in abundance were the Oxystigma (Odonata) and Corydalus (Megaloptera) genera. In March 2017 (higher precipitation/temperature period), four exclusive taxa were recorded, amongst them, the Chimarra (Trichoptera) genus. Furthermore, seasonal records demonstrated higher occurrences and abundance of macroinvertebrates during the intermediate and lower precipitation/temperature periods, besides a varied taxa composition throughout the year, with the presence of both sensitive and tolerant groups to environmental impacts. Our findings suggest that the number and composition of the local-stream macrobenthic fauna were influenced by the seasonal climatic regime. These changes should be considered in the limnological monitoring developed on the hydrographic systems of INP eastern edges to improve the assessment of environmental quality under different local seasonal conditions.

Biological dinitrogen (N2) fixation is performed solely by specialized bacteria and archaea termed diazotrophs, introducing new reactive nitrogen into aquatic environments. Conventionally, phototrophic cyanobacteria are considered the major diazotrophs in aquatic environments. However, accumulating evidence indicates that diverse non-cyanobacterial diazotrophs (NCDs) inhabit a wide range of aquatic ecosystems, including temperate and polar latitudes, coastal environments and the deep ocean. NCDs are thus suspected to impact global nitrogen cycling decisively, yet their ecological and quantitative importance remain unknown. Here we review recent molecular and biogeochemical evidence demonstrating that pelagic NCDs inhabit and thrive especially on aggregates in diverse aquatic ecosystems. Aggregates are characterized by reduced-oxygen microzones, high C:N ratio (above Redfield) and high availability of labile carbon as compared to the ambient water. We argue that planktonic aggregates are important loci for energetically-expensive N2 fixation by NCDs and propose a conceptual framework for aggregate-associated N2 fixation. Future studies on aggregate-associated diazotrophy, using novel methodological approaches, are encouraged to address the ecological relevance of NCDs for nitrogen cycling in aquatic environments.

Freshwater ecosystems and fishes are enormous resources for human uses and biodiversity worldwide. However, anthropogenic climate change and factors such as dams and environmental contaminants threaten these freshwater systems. One way that researchers can address conservation issues in freshwater fishes is via integrative non-lethal movement research. We review different methods for studying movement, such as with acoustic telemetry. Methods for connecting movement and physiology are then reviewed, by using non-lethal tissue biopsies to assay environmental contaminants, isotope composition, protein metabolism, and gene expression. Methods for connecting movement and genetics are reviewed as well, such as by using population genetics or quantitative genetics and genome-wide association studies. We present further considerations for collecting molecular data, the ethical foundations of non-lethal sampling, integrative approaches to research, and management decisions. Ultimately, we argue that non-lethal sampling is effective for conducting integrative, movement-oriented research in freshwater fishes. This research has the potential for addressing critical issues in freshwater systems in the future.

Abstract Microbes in snow and ice ecosystems in polar regions contribute substantially to C, N, and P cycling, but few studies have explored microbial activity in seasonal snow. The purpose of this study was to explore the relative importance of snow microbial processing of C, N, and P compounds in atmospheric deposition and litter and detect elemental limitations of snow microbes in Rocky Mountain conifer forests. Enzyme activity in snow was orders of magnitude greater than activity reported for lentic and lotic waters in similar environments. Proportions of C/P‐ and C/N‐acquiring enzymes suggest that snow samples were P limited, or C and P co‐limited, while lentic and lotic waters were more N limited. As such, microbes in seasonal snow may change the composition of nutrients and carbon, but these processes are vulnerable to changes in atmospheric deposition and snow extent and duration, which could affect nutrient processing across large areas.

Over the 5-year surveillance period, from July 2019 through June 2024, the crude incidence rate of any cold weather injury was 31.1 per 100,000 person-years (p-yrs) for the active component and 6.4 per 100,000 persons for the reserve component of the U.S. Armed Forces. From July 2023 through June 2024, a total of 456 members of the active (n=403) and reserve (n=53) components of the U.S. Armed Forces had at least 1 cold weather injury. During the 2023-2024 cold season, the rates of any cold weather injury increased slightly for the active (31.1 per 100,000 p-yrs) and reserve (6.4 per 100,000 persons) components compared to the previous cold season. The rate of any cold weather injury varied among the services for the 2023-2024 cold season, with higher rates observed in the Army and the Marine Corps. Over the entire surveillance period, active component service member cold weather injury rates were higher among males, non-Hispanic Black or African Americans, and those under 20 years old.