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703 results on '"Zhou, Zhuan"'

203. Stretch-induced Ca2+ independent ATP release in hippocampal astrocytes.

Author

Xiong, Yingfei, Teng, Sasa, Zheng, Lianghong, Sun, Suhua, Li, Jie, Guo, Ning, Li, Mingli, Wang, Li, Zhu, Feipeng, Wang, Changhe, Rao, Zhiren, and Zhou, Zhuan

Subjects
ASTROCYTES, NEURAL circuitry, NEURAL transmission, PANNEXINS, EXOCYTOSIS
Abstract

Astrocytic ATP release is essential for brain functions such as synaptic long-term potentiation for learning and memory. However, whether and how ATP is released via exocytosis remains hotly debated. All previous studies of non-vesicular ATP release have used indirect assays. By contrast, two recent studies report vesicular ATP release using more direct assays. In the present study, using patch clamped 'ATP-sniffer cells', we re-investigated astrocytic ATP release at single-vesicle resolution in hippocampal astrocytes. Following an 'on-cell-like' mechanical stimulus of a single astrocyte, a Ca2+ independent single large non-quantal ATP release occurred, in contrast to the Ca2+-dependent multiple small quantal ATP release in a chromaffin cell. The mechanical stimulation-induced ATP release from an astrocyte was inhibited by either exposure to a selective antagonist or genetic knockdown of P2X7 receptor channels. Functional P2X7 channels were expressed in astrocytes in hippocampal brain slices. Thus, in addition to small quantal ATP release, larger non-quantal ATP release depends on P2X7 channels in astrocytes. [ABSTRACT FROM AUTHOR]

Published
2018
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204. FEM-DEM coupling simulations of the tool wear characteristics in prestressed machining superalloy

Author

Tang Heng, Zhou Zhuan, Tang Xinzi, and Peng Ruitao

Subjects
Sialon, Materials science, business.industry, 020502 materials, 02 engineering and technology, Structural engineering, Finite element method, Superalloy, 0205 materials engineering, Machining, lcsh:TA1-2040, visual_art, visual_art.visual_art_medium, Coupling (piping), Ceramic, Boundary value problem, Tool wear, lcsh:Engineering (General). Civil engineering (General), business
Abstract

Due to the complicated contact loading at the tool-chip interface, ceramic tool wear in prestressed machining superalloy is rare difficult to evaluate only by experimental approaches. This study aims to develop a methodology to predict the tool wear evolution by using combined FEM and DEM numerical simulations. Firstly, a finite element model for prestressed cutting is established, subsequently a discrete element model to describe the tool-chip behaviour is established based on the obtained boundary conditions by FEM simulations, finally, simulated results are experimentally validated. The predicted tool wear results show nice agreement with experiments, the simulation indicates that, within a certain range, higher cutting speed effectively results in slighter wear of Sialon ceramic tools, and deeper depth of cut leads to more serious tool wear.

Published
2016

205. Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663

Author

Wang Jian, Wu Shui Yan, Pan Jian, Zhou Zhuan, Tao Yan Fang, Liao Xin Mei, Zhao Wen Li, and Ni Jian

Subjects
Cancer Research, Time Factors, Cellular differentiation, Lymphocyte, Retinoic acid, Antineoplastic Agents, HL-60 Cells, Tretinoin, Biology, Transfection, lcsh:RC254-282, chemistry.chemical_compound, Downregulation and upregulation, microRNA, medicine, Humans, Promoter Regions, Genetic, Molecular Biology, Spleen Focus-Forming Viruses, Cell Proliferation, Oligonucleotide Array Sequence Analysis, lcsh:RC633-647.5, Gene Expression Regulation, Leukemic, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, Research, Lentivirus, Myeloid leukemia, Cell Differentiation, lcsh:Diseases of the blood and blood-forming organs, Hematology, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Up-Regulation, MicroRNAs, medicine.anatomical_structure, Oncology, chemistry, Cell culture, Cancer research
Abstract

Background Differentiation of the acute myeloid leukemia (AML) cell line HL-60 can be induced by all trans-retinoic acid (ATRA); however, the mechanism regulating this process has not been fully characterized. Methods Using bioinformatics and in vitro experiments, we identified the microRNA gene expression profile of HL-60 cells during ATRA induced granulocytic differentiation. Results Six microRNAs were upregulated by ATRA treatment, miR-663, miR-494, miR-145, miR-22, miR-363* and miR-223; and three microRNAs were downregulated, miR-10a, miR-181 and miR-612. Additionally, miR-663 expression was regulated by ATRA. We used a lentivirus (LV) backbone incorporating the spleen focus forming virus (SFFV-F) promoter to drive miR-663 expression, as the CMV (Cytomegalovirus) promoter is ineffective in some lymphocyte cells. Transfection of LV-miR-663 induced significant HL-60 cell differentiation in vitro. Conclusions Our results show miR-663 may play an important role in ATRA induced HL-60 cell differentiation. Lentivirus delivery of miR-663 could potentially be used directly as an anticancer treatment in hematological malignancies

Published
2011

206. MMP28 (epilysin) as a novel promoter of invasion and metastasis in gastric cancer

Author

Wang Jian, Pan Jian, Zhou Zhuan, Zhu Xue-ming, Tao Yanfang, and Ni Jian

Subjects
Cancer Research, Pathology, medicine.medical_specialty, Gene Expression, Mice, Nude, lcsh:RC254-282, Metastasis, Mice, In vivo, Surgical oncology, Stomach Neoplasms, Cell Line, Tumor, Matrix Metalloproteinases, Secreted, Genetics, medicine, Animals, Humans, Neoplasm Invasiveness, Neoplasm Metastasis, Cell Proliferation, business.industry, Cell growth, Gene Expression Profiling, Carcinoma, Cancer, medicine.disease, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Survival Analysis, Xenograft Model Antitumor Assays, Oncology, Cancer cell, Immunohistochemistry, Ectopic expression, business, Research Article
Abstract

Background The purpose of this study was to investigate invasion and metastasis related genes in gastric cancer. Methods The transwell migration assay was used to select a highly invasive sub-line from minimally invasive parent gastric cancer cells, and gene expression was compared using a microarray. MMP28 upregulation was confirmed using qRT-PCR. MMP28 immunohistochemistry was performed in normal and gastric cancer specimens. Invasiveness and tumor formation of stable cells overexpressing MMP28 were tested in vitro and in vivo. Results MMP28 was overexpressed in the highly invasive sub-cell line. Immunohistochemistry revealed MMP28 expression was markedly increased in gastric carcinoma relative to normal epithelia, and was significantly associated with depth of tumor invasion, lymph node metastasis and poorer overall survival. Ectopic expression of MMP28 indicated MMP28 promoted tumor cell invasion in vitro and increased gastric carcinoma metastasis in vivo. Conclusions This study indicates MMP28 is frequently overexpressed during progression of gastric carcinoma, and contributes to tumor cell invasion and metastasis. MMP28 may be a novel therapeutic target for prevention and treatment of metastases in gastric cancer.

Published
2010

208. Striatal dopamine release in a schizophrenia mouse model measured by electrochemical amperometry in vivo

Author

Xu, Huadong, primary, Zuo, Panli, additional, Wang, Shirong, additional, Zhou, Li, additional, Sun, Xiaoxuan, additional, Hu, Meiqin, additional, Liu, Bin, additional, Wu, Qihui, additional, Dou, Haiqiang, additional, Liu, Bing, additional, Zhu, Feipeng, additional, Teng, Sasa, additional, Zhang, Xiaoyu, additional, Wang, Li, additional, Li, Qing, additional, Jin, Mu, additional, Kang, Xinjiang, additional, Xiong, Wei, additional, Wang, Changhe, additional, and Zhou, Zhuan, additional

Published
2015
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209. Dual Spikes of Catecholamine Releases from Sympathetic Nerves in Rodent Heart Slices Following Hypoxia-Reperfusion as Recorded by a Novel Electrochemical Method

Author

Liu, Bing, primary, Guo, Shu, additional, Li, Jing, additional, Kang, Xinjiang, additional, Xiu, Yun, additional, Gu, Jingli, additional, Mu, Yu, additional, Lei, Qian, additional, Liu, Bin, additional, Liu, Kun, additional, Li, Lihuan, additional, Wang, Changhe, additional, Cao, Jimin, additional, and Zhou, Zhuan, additional

Published
2015
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211. Calcium influx activates adenylyl cyclase 8 for sustained insulin secretion in rat pancreatic beta cells

Author

Dou, Haiqiang, primary, Wang, Changhe, additional, Wu, Xi, additional, Yao, Lijun, additional, Zhang, Xiaoyu, additional, Teng, Sasa, additional, Xu, Huadong, additional, Liu, Bin, additional, Wu, Qihui, additional, Zhang, Quanfeng, additional, Hu, Meiqin, additional, Wang, Yeshi, additional, Wang, Li, additional, Wu, Yi, additional, Shang, Shujiang, additional, Kang, Xinjiang, additional, Zheng, Lianghong, additional, Zhang, Jin, additional, Raoux, Matthieu, additional, Lang, Jochen, additional, Li, Qing, additional, Su, Jing, additional, Yu, Xiao, additional, Chen, Liangyi, additional, and Zhou, Zhuan, additional

Published
2014
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213. Fuzzy matrix analysis of the maximum road in traffic network

Author

Ding Xiangqian, Zhou Zhuan, and Liu Wenbin

Subjects
Physics::Physics and Society, Mathematical optimization, Traffic congestion reconstruction with Kerner's three-phase theory, Fuzzy set, Fuzzy logic, Power (physics), Intelligent Network, Flow (mathematics), Fuzzy matrix, ComputerSystemsOrganization_MISCELLANEOUS, Computer Science::Networking and Internet Architecture, Traffic generation model, Simulation, Mathematics
Abstract

In this paper, the concepts of fuzzy traffic network, road width and maximum road are introduced. Based on the fuzzy matrix representing traffic network the connection between the maximum road width and the power operation of the fuzzy matrix is discussed. And then the arithmetic solving method and the implemental program for the maximum road are introduced. They have practical value in analyzing traffic network such as regulating the flow of traffic in cities.

Published
2004

217. Extracellular Ca2+ per se inhibits quantal size of catecholamine release in adrenal slice chromaffin cells

Author

Shang, Shujiang, primary, Wang, Changhe, additional, Liu, Bin, additional, Wu, Qihui, additional, Zhang, Quanfeng, additional, Liu, Wei, additional, Zheng, Lianghong, additional, Xu, Huadong, additional, Kang, Xinjiang, additional, Zhang, Xiaoyu, additional, Wang, Yeshi, additional, Zheng, Hui, additional, Wang, Shirong, additional, Xiong, Wei, additional, Liu, Tao, additional, and Zhou, Zhuan, additional

Published
2014
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220. Modulation of dopamine release in the striatum by physiologically relevant levels of nicotine

Author

Wang, Li, primary, Shang, Shujiang, additional, Kang, Xinjiang, additional, Teng, Sasa, additional, Zhu, Feipeng, additional, Liu, Bin, additional, Wu, Qihui, additional, Li, Mingli, additional, Liu, Wei, additional, Xu, Huadong, additional, Zhou, Li, additional, Jiao, Ruiying, additional, Dou, Haiqiang, additional, Zuo, Panli, additional, Zhang, Xiaoyu, additional, Zheng, Lianghong, additional, Wang, Shirong, additional, Wang, Changhe, additional, and Zhou, Zhuan, additional

Published
2014
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223. Rescue of Dopamine Release and Behavior by Transplanted Neural Stem Cells in a Rat Model of Parkinsonism

Author

Kang, Xinjiang, primary, Xu, Huadong, additional, Zhou, Li, additional, Zuo, Panli, additional, Deng, Zijun, additional, Liu, Bing, additional, Liu, Bin, additional, Wang, Li, additional, Dou, Haiqian, additional, Zhu, Feipeng, additional, Wang, Changhe, additional, Wang, Shirong, additional, Li, Wenlin, additional, Zhang, Kang, additional, and Zhou, Zhuan, additional

Published
2014
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225. Gain-of-Function Mutations in SCN11A Cause Familial Episodic Pain

Author

Zhang, Xiang Yang, primary, Wen, Jingmin, additional, Yang, Wei, additional, Wang, Cheng, additional, Gao, Luna, additional, Zheng, Liang Hong, additional, Wang, Tao, additional, Ran, Kaikai, additional, Li, Yulei, additional, Li, Xiangyang, additional, Xu, Ming, additional, Luo, Junyu, additional, Feng, Shenglei, additional, Ma, Xixiang, additional, Ma, Hongying, additional, Chai, Zuying, additional, Zhou, Zhuan, additional, Yao, Jing, additional, Zhang, Xue, additional, and Liu, Jing Yu, additional

Published
2013
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226. Real-time endocytosis imaging as a rapid assay of ligand-GPCR binding in single cells

Author

Zheng, Liang-Hong, primary, Wang, Chang-He, additional, Shang, Shu-Jiang, additional, Zhang, Xiao-Yu, additional, Wang, Ye-Shi, additional, Wu, Qi-Hui, additional, Hu, Mei-Qin, additional, Chai, Zu-Yin, additional, Wu, Xi, additional, Zheng, Hui, additional, Zhang, Chen, additional, Wang, Lie-Cheng, additional, Xiong, Wei, additional, and Zhou, Zhuan, additional

Published
2013
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229. Cocaine Preferentially Potentiates Fast Releasable Vesicle Pool in Mouse Dopaminergic Striatum In Vivo

Author

Zuo, Pan-Li, primary, Kang, Xin-Jiang, additional, Xu, Hua-Dong, additional, Zhang, Bo, additional, Zhou, Li, additional, Dou, Hai-Qiang, additional, Zhu, Fei-Peng, additional, Liu, Li-Na, additional, Guo, Shu, additional, Lü, Jin, additional, Li, Qing, additional, Wang, Shi-Rong, additional, Yao, Wei, additional, Gu, Howard, additional, and Zhou, Zhuan, additional

Published
2013
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230. Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis.

Author

Wang, Changhe, Wang, Yeshi, Hu, Meiqin, Chai, Zuying, Wu, Qihui, Huang, Rong, Han, Weiping, Zhang, Claire Xi, and Zhou, Zhuan

Abstract

Precise and efficient endocytosis is essential for vesicle recycling during a sustained neurotransmission. The regulation of endocytosis has been extensively studied, but inhibitors have rarely been found. Here, we show that synaptotagmin-11 (Syt11), a non-Ca2+-binding Syt implicated in schizophrenia and Parkinson's disease, inhibits clathrin-mediated endocytosis (CME) and bulk endocytosis in dorsal root ganglion neurons. The frequency of both types of endocytic event increases in Syt11 knockdown neurons, while the sizes of endocytosed vesicles and the kinetics of individual bulk endocytotic events remain unaffected. Specifically, clathrin-coated pits and bulk endocytosis-like structures increase on the plasma membrane in Syt11-knockdown neurons. Structural- functional analysis reveals distinct domain requirements for Syt11 function in CME and bulk endocytosis. Importantly, Syt11 also inhibits endocytosis in hippocampal neurons, implying a general role of Syt11 in neurons. Taken together, we propose that Syt11 functions to ensure precision in vesicle retrieval, mainly by limiting the sites of membrane invagination at the early stage of endocytosis. [ABSTRACT FROM AUTHOR]

Published
2016
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232. GW24-e0758 New insights into cardiomyocyte differentiation from adipose-derived stem cells, promoting understanding of brown adipocyte

Author

Zhang Chuansen, Zhou Zhuan, Deng Zijun, Chen Lei, Li Yuquan, Yang Xiangqun, Kang Xinjiang, Wang Changhe, and Chai Zuyin

Subjects
medicine.medical_specialty, medicine.diagnostic_test, Brown Adipocytes, business.industry, Regeneration (biology), Cellular differentiation, Adipose tissue, Immunofluorescence, In vitro, Cell biology, Endocrinology, medicine.anatomical_structure, Internal medicine, Brown adipose tissue, medicine, Stem cell, Cardiology and Cardiovascular Medicine, business
Abstract

Objectives To investigate and compare the ability of stem cells from white and brown adipose tissue spontaneous differentiation into cardiomyocytes in vitro , and develop new way to heart regeneration. Methods The differentiated cardiomyocyte-like cells were identified bymorphological observation, confirmed with expression of specific cardiac genes, immunofluorescence and ultrastructural analysis. Electrophysiological studieshave shown the pacemaker activity of the differentiated cells. Results Here, we found that brown adipose-derived stem cells (ASCs) could differentiated into beating cardiomyocytes spontaneously in vitro , but not the white ASCs. Likewise, mature brown adipocytes can trans differentiate into spontaneously beating cardiomyocytes. Conclusions Our result not only confirmed the brown adipocytes can differentiate into cardiomyocytes spontaneously in vitro but also offered new insights into cardiomyocyte differentiation and regeneration.

Published
2013

238. Action potential-triggered somatic exocytosis in mesencephalic trigeminal nucleus neurons in rat brain slices

Author

Zhang, Bo, primary, Zhang, Xiao-Yu, additional, Luo, Pi-Fu, additional, Huang, Wei, additional, Zhu, Fei-Peng, additional, Liu, Tao, additional, Du, Yi-Ru, additional, Wu, Qi-Hui, additional, Lü, Jin, additional, Xiu, Yun, additional, Liu, Li-Na, additional, Huang, Hong-Ping, additional, Guo, Shu, additional, Zheng, Hui, additional, Zhang, Claire Xi, additional, and Zhou, Zhuan, additional

Published
2012
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246. Role of vesicle pools in action potential pattern-dependent dopamine overflow in rat striatum in vivo

Author

Wang, Shi-Rong, primary, Yao, Wei, additional, Huang, Hong-Ping, additional, Zhang, Bo, additional, Zuo, Pan-Li, additional, Sun, Liang, additional, Dou, Hai-Qiang, additional, Li, Qing, additional, Kang, Xin-Jiang, additional, Xu, Hua-Dong, additional, Hu, Mei-Qin, additional, Jin, Mu, additional, Zhang, Lei, additional, Mu, Yu, additional, Peng, Ji-Yun, additional, Zhang, Claire Xi, additional, Ding, Jiu-Ping, additional, Li, Bao-Ming, additional, and Zhou, Zhuan, additional

Published
2011
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