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To investigate the correlation of the expression of VEGF-C and VEGFR-3 to the pathological grade of human prostate cancer.Immunohistochemistry was used to detect the expression of VEGF-C and VEGFR-3 in 25 cases of prostate cancer tissues.The total positivity rates of VEGF-C and VEGFR-3 were 80% and 76% in these cancer tissues, respectively. The positivity rates of VEGF-C was 94.7% in the 19 cases with Gleason scores no less than 6 (group I), significantly higher than the rate (33%) in the 6 cases with Gleason scores between 4 and 6 (group II) (P0.01). The positivity rates for VEGFR-3 also showed a significant difference between groups I and II (89.5% vs 33.3%, P0.05). The expression level of VEGF-C was correlated to the Gleason score of prostate cancer (R=0.436, P0.05), and the correlation between VEGFR-3 and the Gleason score was even more obvious (R=0.608, P0.01). Their expressions, however, did not show any correlations to the patients age, PSA or the volume of the prostate.VEGF-C and VEGFR-3 may serve as new markers for evaluating the malignancy of prostate cancer with Gleason score not less than 4.

To evaluate the value of multi-slice CT and MRI on maxillofacial region soft tissue measurement.A total of 12 cases of dentofacial orthodontic underwent X-ray photograph, multi-slice CT and MRI scanning. The angle and distance of maxillofacial region soft tissue were measured respectively. All data were analyzed statistically for ANOVA with SPSS11.0 software package.There was no significant difference among the three measurement methods: X-ray photograph, multi-slice CT and MRI(P0.05). MRI had advantages on depicting hierarchical structure of maxillofacial region, especially soft tissues, while multi-slice CT had advantages on depicting structure of maxillofacial region, both hard and soft tissues.Multi-slice CT and MRI are useful on maxillofacial region soft tissues measurement. MRI is superior to multi-slice CT and X-ray photograph on maxillofacial region soft tissue measurement.

MicroRNA (miRNA), which is short non-coding RNA, plays important roles in almost all biological processes examined. Several classifiers have been applied to predict humans, mice and rats precursor miRNAs (pre-miRNAs), but no classifier is applied to classify porcine pre-miRNAs only based on the porcine pre-miRNAs because of little known miRNA component in the porcine genome. Here, we developed a novel classifier, called SusMiRPred, to predicted porcine pre-miRNAs. Trained on 60 porcine pre-miRNAs and 65 pseudo procine hairpins, SusMiRPred achieve 86.4% (5-fold cross-validation accuracy) and 0.9144 (ROC score). Tested on the remaining 14 porcine pre-miRNAs and 1000 pseudo hairpins, it reports 100% (sensitivity), 87.3% (specificity) and 87.5% (accuracy). SusMiRPred was proved an effective ab initio Support Vector Machine (SVM) classifier for predicting porcine pre-miRNAs and encapsulated with a Java package for other users utilizing it expedient. Furthermore, another Java package, called SusMiRFilter, was developed to filter out the short sequences which have not the pre-miRNAs sequence structure features.

The water quality and algae community of Nanyuan Water System in the old city area of Suzhou were monitored for a year. Results showed that the water pollution in the studied area was mainly related to nitrogen (NH4+ -N and TN). Sometimes, they even exceeded the Environmental Quality Standards for Surface Water (GB 3838-2002, PRC) more than 5 times. 34 species of benthic epipelic algae were observed by microscope, and the species amount of diatom algae, green algae and blue algae are more than others. Their abundance and biomass are far higher than that of the pelagic algae in the same sites,and reach 2 145.5 x 10(4) cells/mL and 3.524 mg/mL,respectively. The dominant species of benthic epipelic algae in Nanyuan's water system are diatom algae and blue algae, most of which belong to the heterotrophic type or bi-trophic type algae, the typical genera include: Oscillaria amphibian (affiliated to Cyanophyta), Cyclotella sp., Melosira sp., Stephanodiscus hantzschii, Navicula sp., Nitzschia sp., Gomphonema (affiliated to Bacillariophyta) and so on. And their distribution of species and abundance are very nonuniform in different reach of heavily polluted city river, which relates to the pollutant characteristics of the river. The seasonal variety trend of the abundance for benthic algae showed that:summerautumnspringwinter, and that of biomass for benthic algae showed that: the biomass in winter is the most of four seasons and change extent of the biomass is not obvious in spring, summer and autumn. The research results can provide reference for the ecology restoration of city heavily polluted river.

In this paper, we mainly investigate the problems of resonances of massive KK fermions on a single scalar constructed thick brane with a piecewise warp factor matching smoothly. The distance between two boundaries and the other parameters are determined by one free parameter through three junction conditions. For the generalized Yukawa coupling $\eta\bar{\Psi}\phi^{k}\Psi$ with odd $k=1,3,5,...$, the mass eigenvalue $m$, width $\Gamma$, lifetime $\tau$, and maximal probability $P_{max}$ of fermion resonances are obtained. Our numerical calculations show that the brane without internal structure also favors the appearance of resonant states for both left- and right-handed fermions. The scalar-fermion coupling and the thickness of the brane influence the resonant behaviors of the massive KK fermions., Comment: V3: 15 pages, 7 figures, published version

A few years ago, Campos investigated the critical phenomena of thick branes in warped spacetimes [Phys. Rev. Lett. {\bf 88} (2002) 141602]. Inspired by his work, we consider a toy model of thick branes generated by a real scalar field with the potential $V(\phi)=a\phi^2-b\phi^4+c\phi^6$, and investigate the variation of the mass parameter $a$ on the branes as well as the localization and resonances of fermions. An interesting result is found: there is a critical value for the mass parameter $a$, and when the critical value of $a$ is reached the solution of the background scalar field is not unique and has the shape of a double kink. This happens in both cases with and without gravity. It is also shown that the numbers of the bound Kaluza-Klein modes of fermions on the gravity-free brane and the resonant states of fermions on the brane with gravity increase with the value of $a$., Comment: 10 pages, 12 figures, published version

The relationship between urinary 1-hydroxypyrene and ambient polynuclear aromatic hydrocarbons (PAHs) was investigated with several groups of volunteers carrying personal air samplers. All the results demonstrate that there is a statistically significant correlation between 1-hydroxypyrene in human urine and pyrene and benzo(a)pyrene in ambient air. Smoking was found not to interfere this correlation when the smokers consume less than 20 cigarettes daily. Different sources of PAH pollution, such as certain industries and coal-burning, present their influence on 1-hydroxypyrene in their specific ways. It is suggested that 1-hydroxypyrene in human urine is an effective biological monitoring index for the assessment of human exposure to PAHs. 18 refs., 7 figs., 6 tabs.

The kinetic release behaviors of a-endosulfan from red soil with three kinds of low-molecular-weight organic acids (LMWOA: oxalate, tartrate and citrate) solution and water leaching were investigated by kinetic device designed by ourselves and batch method. The results show that: the release percentage of endosulfan from red soil by tartrate and citrate solution (10 mmol/L) can increase by 7%-18% more than that by distilled water and oxalate solution, especially for tartrate solution. There is no significant difference between distilled water and oxalate solution for the release percentage of endosulfan (p0.05). There are two stages of quick and slow for the release of endosulfan from red soil, and the leaching speed is quicker especially for the initial 200 mL leaching solution. When using distilled water or oxalate solution as leaching solution, the best equations that described the kinetic release behavior of endosulfan from red soil were parabola diffuse equation and double constant equation, and weren't the apparent first dynamics equation that represented the simple surface diffusion mechanism. The kinetic release behavior of endosulfan in tartrate or citrate leaching system can be described by Elovich equation (R20.99, p0.0001), it implied that the simple surface diffusion mechanism is not the primary factor that effected the release of endosulfan, which three-dimensional molecule structure is complex, from red soil in aqueous phase leaching systems, and it maybe related to the outward diffuse mechanism from soil particle, activation and deactivation function of soil particles surface, the dissolution of soil mineral surface and structure change of inherent organic matter that coating onto the soil mineral surface induced by LMW organic acid. It suggested that the tartrate and citrate induced the complication of the release mechanisms of the pesticides from red soil.

In arXiv:0901.3543, the simplest Yukawa coupling $\eta\bar{\Psi}\phi\chi\Psi$ was considered for a two-scalar-generated Bloch brane model. Fermionic resonances for both chiralities were obtained, and their appearance is related to branes with internal structure. Inspired on this result, we investigate the localization and resonance spectrum of fermions on a one-scalar-generated $dS$ thick brane with a class of scalar-fermion couplings $\eta\bar{\Psi}\phi^k\Psi$ with positive odd integer $k$. A set of massive fermionic resonances for both chiralities are obtained when provided large couple constant $\eta$. We find that the masses and life-times of left and right chiral resonances are almost the same, which demonstrates that it is possible to compose massive Dirac fermions from the left and right chiral resonances. The resonance with lower mass has longer life-time. For a same set of parameters, the number of resonances increases with $k$ and the life-time of the lower level resonance for larger $k$ is much longer than the one for smaller $k$., Comment: 23 pages, 35 figures, discussion and references added, to appear in PRD

Motivated by the recent work on the fermion resonances on scalar-constructed thick branes (arXiv:0901.3543 and arXiv:0904.1785), we extend the idea to multi-scalar generated thick branes and complete previous work. The fermion localization and resonances on the three-field and two-field thick branes are investigated. With the Numerov method, our numerical results show that the resonance states also exist in the brane besides the single-field thick branes and the two-field thick branes in the former cases. This interesting phenomenon is related to the internal structure of the brane and the coupling of fermions and scalars. We find that the Kaluza-Klein chiral decomposition of massive fermion resonances is the parity-chiral decomposition. For the couplings $\eta\bar{\Psi}\phi^{k}\chi\rho\Psi$ and $\eta\bar{\Psi}\phi^{k}\chi\rho\Psi$ for three-field and two-field models with odd positive $k$, respectively, the number of the resonant states decreases with $k$. This result is opposite to the one obtained in the single-field de Sitter thick brane (arXiv:0904.1785)., Comment: 30 pages, 17 figures, to appear in JHEP

The root of Fallopia multiflora is one of the most widely used traditional Chinese medicines. However, it is often confused and substituted with the roots of F. multiflora var. ciliinervis, Pteroxygonum giraldii, Cynanchum auriculatum, and Stephania cepharantha. To establish a DNA polymorphism-based assay for the identification of F. multiflora, the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) regions of six Fallopia species were sequenced and analyzed. Based on the diversity of ITS regions among the species the diagnostic primers PMITS28 and PMITS545, which amplified an expected 517-bp DNA fragment from F. multiflora DNA, were designed. No amplified product was observed when DNA from other species was used. This method can be used for the authentication of F. multiflora.

Field investigations on the phytoplankton community were carried out in December 2005 and June 2006 in shallow lakes of plainriver network areas, Suzhou City. Results show that there are 73 species, 62 genus, 8 phylum phytoplankton, which is mainly composed of the Chlorophyta, Bacillariophyta and Cyanophyta. In winter, the average phytoplankton abundance is 254.88 x 10(4) cells x L(-1), and the Bacillariophyta abundance accounts for 62.3% of the total. While in summer, the average phytoplankton abundance is 2704.28 x 10(4) cells x L(-1), and the Cyanophyta abundance accounts for 93.5%. The relationship between 62 genus of phytoplankton and 11 environmental factors from 57 sampling sites in research area was studied by Canonical Correspondence Analysis. It suggests that water temperature, permanganate indexes, nitrate nitrogen and total nitrogen are the main environmental factors correlated with the distribution of phytoplankton community. And in winter, pH, ammonia nitrogen and total phosphorus are also the main environmental factors. Furthermore, the Bacillariophyta has higher adaptability to the changeable environment. The Chlorophyta can tolerate higher concentration of permanganate indexes, phosphorus nutrients, nitrogen nutrients and total organic carbon, whereas the response of Cyanophyta to environmental factors needs further research.

An asymmetric thick domain wall solution with de Sitter ($dS$) expansion in five dimensions can be constructed from a symmetric one by using a same scalar (kink) with different potentials. In this paper, by presenting the mass-independent potentials of Kaluza--Klein (KK) modes in the corresponding Schr\"{o}dinger equations, we investigate the localization and mass spectra of various bulk matter fields on the symmetric and asymmetric $dS$ thick branes. It is shown that the spectrum of scalar KK modes on the symmetric $dS$ brane contains only one bound mode (the massless mode). However, for the asymmetric $dS$ brane with a large asymmetric factor $a$, there are two bound scalar KK modes: a zero mode and a massive mode. For spin 1 vectors, the spectra of KK modes on both $dS$ branes consist of a bound massless mode and a set of continuous ones, i.e., the asymmetric factor does not change the number of the bound vector KK modes. For spin 1/2 fermions with the scalar-fermion coupling $\eta\bar\Psi \sin(\phi/\phi_0)\cos^{-\delta}(\phi/\phi_0)\Psi$, there exist some discrete bound KK modes and a series of continuous ones. The asymmetric factor $a$ reduces the number of the bound fermion KK modes., Comment: 28 pages, 28 figures

Our objective was to investigate the association between senataxin mutations and sporadic amyotrophic lateral sclerosis (ALS) in Chinese patients. DNA from 45 sporadic ALS patients was screened for mutations in senataxin using polymerase chain reaction (PCR) and direct sequencing. A novel variation, Thr1118Ile, was identified in a 42-year-old individual with sporadic ALS. This variation was not detected in 200 unrelated control individuals. In conclusion, the presence of this variation in a patient with sporadic ALS, and its absence in 200 controls, supports an association between senataxin and sporadic ALS. This study has broadened the mutation spectrum of senataxin and expanded the clinical phenotypes of senataxin mutations.

The first order perturbations of the energy levels of a stationary hydrogen atom in static external gravitational field, with Schwarzschild metric, are investigated. The energy shifts are calculated for the relativistic 1S, 2S, 2P, 3S, 3P, 3D, 4S, 4P, 4D and 4F levels. The results show that the energy-level shifts of the states with total angular momentum quantum number 1/2 are all zero, and the ratio of absolute energy shifts with total angular momentum quantum number 5/2 is 1:4:5. This feature can be used to help us to distinguish the gravitational effect from other effect., 11 pages

The first order perturbations of the energy levels of a hydrogen atom in central internal gravitational field are investigated. The internal gravitational field is produced by the mass of the atomic nucleus. The energy shifts are calculated for the relativistic 1S, 2S, 2P, 3S, 3P, 3D, 4S and 4P levels with Schwarzschild metric. The calculated results show that the gravitational corrections are sensitive to the total angular momentum quantum number., 7 pages

Xu-Dong Che ⁎, Fu-Yuan Wu , Ru-Cheng Wang , Axel Gerdes , Wei-Qiang Ji , Zhen-Hua Zhao , Jin-Hui Yang , Ze-Ying Zhu a a State Key Laboratory for Mineral Deposits Research, School of Earth Sciences and Engineering, Nanjing University, Xianlin University Town, Nanjing 210046, China b State Key Laboratory of Lithospheric Evolution, Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing 100029, China c Institut fur Geowissenschaften, Goethe Universitat Frankfurt, Altenhoeferallee 1, D-60438 Frankfurt am Main, Germany d Guanzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China

The kinetic release behavior of DDTs from red soil with three kinds of low-molecular-weight (LMW) organic acids solution and water leaching was investigated by kinetic device designed. The results show that the release percentage of DDTs from red soil by LMW organic acid can increase by 15% - 18% more than that by water, especially for the initial 250 mL leaching solution. When using water as leaching solution, the data of release kinetics of DDTs from red soil conformed to the apparently first dynamics equation (R20.99, p0.000 1), it implied that the surface diffuse of soil mineral is primary release mechanism of DDTs from red soil in water leaching system. The kinetic release behavior of DDTs in LMW organic acid leaching system can be described by parabola diffuse equation, double constant equation or Elovich equation. It implied that the LMW organic acids induced the complication of the release mechanisms of DDTs from red soil, this maybe related to the dissolution of soil mineral surface and structure change of inherent organic material that coating onto the soil mineral surface induced by LMW organic acid.

In situ identification of ANAMMOX bacteria was conducted using 16S rRNA approach for sediment samples from the Xinyi River in Jiangsu Province, China. 16S rRNA clone library including 6 clone sequences was constructed. The alignment of these sequences and treeing were conducted using ARB package. Results show that the sediment samples contained 16S rRNA genes closely related to the known ANAMMOX bacterium Candidatus "Brocadia anammoxidans" (similarity of 91%). They also contained 16S rRNA gene sequences from a new branch of Planctomycetes distantly related to the ANAMMOX sequence cluster. However, the microbiological characteristics of these Planctomycetes are to be studied in the future. The detection of ANAMMOX bacteria will lead to further research on ANAMMOX process in the remediation and restoration of freshwater aquatic ecosystems and new understanding of the nitrogen cycle.

The study was designed to compare the combustion products of coal gas, liquefied petroleum gas and natural gas in relation to indoor air pollution.Regular pollutants including B(a)P were monitored and 1-hydroxy pyrene were tested in urine of the enrolled subjects. Radon concentrations and their changes in four seasons were also monitored in the city natural gas from its source plant and transfer stations to final users. To analyze organic components of coal gas, liquefied petroleum gas and natural gas, a high-flow sampling device specially designed was used to collect their combustion products, and semi-volatile organic compounds contained in the particles were detected by gas chromatograph-mass spectrograph (GC/MS).Findings in the study showed that the regular indoor air pollutants particles and CO were all above the standard in winter when heating facilities were operated in the city, but they were lowest in kitchens using natural gas; furthermore, although NO2 and CO2 were slightly higher in natural gas, B(a)P concentration was lower in this group and 1-hydroxy pyrene was lowest in urine of the subjects exposed to natural gas. Organic compounds were more complicated in coal gas and liquefied petroleum gas than in natural gas. The concentration of radon in natural gas accounted for less than 1% of its effective dose contributing to indoor air pollution in Beijing households.Compared to traditional fuels, gases are deemed as clean ones, and natural gas is shown to be cleaner than the other two gases.

Differential display of mRNA was used to identify concordant changes in gene expression induced by two mood-stabilizing agents, lithium and valproate (VPA). Both treatments, on chronic administration, increased mRNA levels of the transcription factor polyomavirus enhancer-binding protein (PEBP) 2beta in frontal cortex (FCx). Both treatments also increased the DNA binding activity of PEBP2 alphabeta and robustly increased the levels of bcl-2 (known to be transcriptionally regulated by PEBP2) in FCx. Immunohistochemical studies revealed a marked increase in the number of bcl-2-immunoreactive cells in layers 2 and 3 of FCx. These novel findings represent the first report of medication-induced increases in CNS bcl-2 levels and may have implications not only for mood disorders, but also for long-term treatment of various neurodegenerative disorders.

In this paper we investigate the localization and mass spectra of bulk matter fields on a Gergory-Rubakov-Sibiryakov-inspired braneworld. In this braneworld model, there are one thick brane located at the origin of the extra dimension and two thin branes at two sides. For spin 1/2 fermions coupled with the background scalar $\phi$ via $\eta\bar{\Psi}\phi^p\Psi$ with $p$ a positive odd integer, the zero mode of left-hand fermions can be localized on the thick brane for finite distance of the two thin branes, and there exist some massive bound modes and resonance modes. The resonances correspond to the quasi-localized massive fermions. For free massless spin 0 scalars, the zero mode can not be localized on the thick brane when the two thin branes are located finitely. While for a massive scalar $\Phi$ coupled with itself and the background scalar field $\phi$, in order to get a localized zero mode on the thick brane, a fine-tuning relation should be introduced. Some massive bound modes and resonances also will appear. For spin 1 vectors, there is no bound KK mode because the effective potential felt by vectors vanishes outside the two thin branes. We also investigate the physics when the distance of the two thin branes tends to infinity.

The concentration of 1-hydroxypyrene in human urine was determined in six volunteers who lived under the same conditions. Samples were collected four times per day for three consecutive days; the maximum concentration of 1-hydroxypyrene was found in the samples collected late in the evening (21:00–23:00 h) which is the time recommended as suitable for determination of urinary 1-hydroxypyrene for the purpose of evaluating human exposure to polycyclic aromatic hydrocarbons (PAHs). Statistical tests show that the influence of sex, age, and smoking on urinary 1-hydroxypyrene is statistically insignificant.

Urinary 1-hydroxypyrene of residents of Beijing, Shenyang and Taiyuan, and workers at two coke plants, a steel plant and control groups was determined using high pressure liquid chromatography. Polycyclic aromatic hydrocarbons (PAHs) of airborne particulates from these cities and coke plants were analyzed using GC-MS; chromatogram profiles, from which 15 chemical compounds were quantified, were similar. The percentage of pyrene and benzo[a]pyrene in PAHs was fairly constant. Statistical analysis showed significant correlation of urinary 1-hydroxypyrene with the concentration of pyrene and benzo[a]pyrene in the ambient PAHs; the correlation coefficient was 0.978 and 0.959, respectively. It is suggested that urinary 1-hydroxypyrene can be used as a biological monitoring index for human exposure to genotoxic PAHs from the burning of coal.

Purpose: To evaluate the efficacy of the Seamless Transfer of Care Model (STCM) to improve readmission occurrence of patients withstroke. Methods: The sample was comprised of fifty-nine subjects with stroke who were hospitalized in the geriatric and neurology departments of a large university hospital in China. Subjects were allocated to an STCM group (n = 30) or a routine care (control) group (n = 29). Results: Compared with the control group, the STCM group had a higher quality of life (p

Late Carboniferous igneous rocks are widespread in the western Tianshan, but the tectonic settings for these rocks remain controversial. We report a plagioclase 40 Ar/ 39 Ar age, and geochemical, Sr–Nd isotope and LA–ICPMS clinopyroxene trace element data for gabbros in the Luotuogou region. The tholeiitic Luotuogou gabbros give a Late Carboniferous (312 ± 1 Ma) 40 Ar/ 39 Ar age and are characterized by high and variable e Nd (t) values ranging from + 3.7 to + 7.8. They have geochemical features of both intra-plate and island arc magmatic rocks, i.e., relatively high TiO 2 (0.6–2.2 wt.%), Nb (4.2–24 ppm) and Zr (51.4–283 ppm) contents combined with variable and slightly high Nb/La ratios (0.24–1.8, mostly > 0.7), and negative to positive Nb anomalies. The gabbros contain zoned clinopyroxenes, with Mg- and Cr-rich cores. Their parental magmas, as calculated using trace element data from Cr-rich (> 3000 ppm) clinopyroxene cores and clinopyroxene/basaltic liquid partition coefficients, show enrichments in incompatible elements, and prominent negative to slightly positive Nb anomalies, indicative of the influence of subduction-related compositions in their mantle source. These features indicate that the Luotuogou gabbros were most likely formed by interactions between asthenospheric and metasomatized lithospheric mantle. They were most plausibly formed by mixing between the asthenospheric mantle-derived and metasomatized lithosphere mantle-derived melts. Mixing was the result of asthenosphere upwelling triggered by roll-back of the subducted Paleo-Junggar Oceanic Plate rather than mantle plume-related rifting or post-collisional break-off during the Late Carboniferous.