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Osteoporotic fractures are a very common bone disease that is difficult to completely cure. A large number of people worldwide suffer from pain caused by osteoporotic fractures every year, which can even cause disability and death. The compromised skeletal strength, lower density, trabecular microstructure, and bone-forming ability caused by osteoporotic fractures make them difficult to treat relative to normal fractures. An ideal scheme for osteoporotic fractures is to select internal fixation materials with matched mechanical and biological properties and carry anti-osteoporosis drugs on the plant to achieve bio-fixation and improve the condition of osteoporosis simultaneously. We designed a Mg-based MAO-MT-PLGA drug/ion delivery system (DDS) compatible with bone-like mechanical properties, degradation properties, and drug therapy. In this research, we evaluated the degradation behavior of Mg-based MAO-MT-PLGA DDS using immersion tests and electrochemical tests aided by SEM, EDS, XPS, XRD, and FT-IR. The DDS showed better corrosion resistance over Mg alloy and could release more Mg2+ due to the degradation of PLGA. According to cell viability and cell adhesion, the DDS showed better osteogenic characteristics over control group I (Mg alloy) and control group II (Mg-based MAO alloy), especially in the cells co-cultured with the leaching solution for 72 h, in which the DDS group increased to about 15% cell viability compared with group I (p < 0.05). The mRNA relative expressions, including ALP, collagen I, OCN, OPG, and Runx-2, as well as extracellular matrix calcium deposits of the DDS, are 1.5~2 times over control group I and control group II (p < 0.05), demonstrating a better ability to promote bone formation and inhibit bone resorption. After the DDS was implanted into the castrated rat model for one month, the trabeculae in the treatment group were significantly denser and stronger than those in the control group, with a difference of about 1.5 times in bone volume fraction, bone density, and the number of trabeculae, as well as the magnesium content in the bone tissue (p < 0.05). The above results demonstrated that the Mg-based MAO-MT-PLGA drug/ion delivery system is a potential treatment for osteoporotic fractures. [ABSTRACT FROM AUTHOR]

The present paper considers the wire drawing process through a conical die and provides an approximate solution for the drawing force, assuming an arbitrary hardening law. The solution is based on the upper bound theorem. However, this theorem does not apply to the stationary flow of strain-hardening materials. Therefore, an adopted engineering approach is to replace the original material model with a non-homogeneous, perfectly plastic model. The kinematically admissible velocity field is derived from an exact semi-analytical solution for the flow through an infinite channel, which increases the accuracy of the solution. The solution for the homogeneous perfectly plastic material compares with an available solution. The general solution is valid for any die angle. The numerical example focuses on the range of angles used in wire drawing. Since the material model is pressure-independent, it is straightforward to adopt the solution for calculating the force in extrusion. [ABSTRACT FROM AUTHOR]

Steam flooding is crucial for the development of heavy oil reservoirs, and the development of the steam cavity significantly determines the efficiency of steam flooding. Previous studies have elucidated the concept of steam overburden and pseudomobility ratio; however, the thermal energy loss in deep heavy oil reservoirs during steam injection needs further investigation. Therefore, in this study, the vapour–liquid interface theory and mathematical integration were used to establish a steam cavity expansion model. The wellbore heat loss rate coefficient, steam overlay, and pseudomobility ratio were used to accurately describe the development of the steam cavity in deep heavy oil reservoirs. The proposed model was experimentally validated, and it was observed that the model could accurately reflect the actual mine conditions. In addition, the pressure gradient distribution of the steam belt and the heat dissipation areas of the top and bottom layers of the steam cavity were evaluated. The results showed that the influence of the wellbore heat loss rate coefficient on the pressure gradient of the oil layer was primarily in the range of 5–20 m away from the steam injection well. Furthermore, it was observed that the pseudomobility ratio is inversely proportional to the development of the steam cavity. As the wellbore heat loss rate coefficient increased, the wellbore heat loss increased. The larger the area ratio, the more pronounced the steam overlay phenomenon, and the large area ratio does not meet the development requirements of the steam chamber. The research closely combines theory with production, and the results of this study can help actual mines by providing theoretical support for the development of deep heavy oil reservoirs. [ABSTRACT FROM AUTHOR]

Metal cylindrical shells are widely used to store and transport highly hazardous chemicals. The impact resistance of metal cylindrical shells under an explosive load is a concern for researchers. In this paper, an innovative failure criterion considering the time effect is proposed for metal cylindrical shells under explosive loads. Firstly, based on the maximum shear stress criterion, an innovative failure criterion containing the time effect is provided. Then, a metal cylindrical shell model is established. Next, a failure pressure equation for metal shells under an explosive load is proposed based on the innovative failure criterion. Lastly, the proposed equation is verified by numerical simulation. The results indicate the failure pressure equation for a metal cylindrical shell under an explosive load uses the finite element method. Our research is of significance for fully understanding the failure mechanism of piping and pressure vessels under impact load. [ABSTRACT FROM AUTHOR]

To reduce the cost of machinery and manual operation, greatly improve the efficiency of maize sowing, and solve the problems of slow sowing speed, unstable operation quality, and the difficult monitoring of the sowing process of traditional seeders, a control system for an electrically driven precision maize seeder based on the CANopen protocol was designed. In this system, an STM32 is used as the main controller, and the vehicle terminal is used to set the operating parameters, such as the spacing of sowing plants and the number of holes in the metering plate. The GPS receiver is used to collect the forward speed of the tractor. An infrared photoelectric sensor is used to monitor the working state of the seeder. In this study, tests were conducted on different evaluation indices. The results showed that the detection accuracy of the photoelectric sensor reached 99.8% and the fault alarm rate reached 100%. The qualified rate of sowing was more than 91.0%. Based on indoor test results, the qualified rate was higher when the grain spacing was larger. The field test showed, in terms of the seeding performance, that the control system had good stability. When the grain spacing was set to 20 cm and the operating speed was 6~12 km/h, the qualified index was more than 89% and the reseeding index was less than 1.93%. The variation in sowing performance between different monomers was small, and the seeding performance was good. The control system helps to improve the performance of the seeder. [ABSTRACT FROM AUTHOR]

In this study, a homemade uniaxial strain pressure cell was designed to be directly used in the standard magnetometers whereby the magnetic properties of samples subjected to a uniaxial strain and magnetic field were characterized. Its feasibility has been demonstrated by the uniaxial strain control of the phase transition and magnetocaloric effect in Ni40Co10Mn40Sn10 (NCMS) alloys. With the assistance of a uniaxial strain of ~0.5%, the cooling temperature span of NCMS alloys is broadened by 2 K, and the refrigeration capacity under a 3 T magnetic field change increases from 246 to 277 J/kg. This research provides not only direct experimental assistance for the tuning of phase transition by the uniaxial strain but also possibilities for studying the coupled caloric effect in first-order phase transition materials under a combined uniaxial strain and magnetic field by the thermodynamic analysis. [ABSTRACT FROM AUTHOR]

Optical signals of pH probes mainly driven from the formation or rupture of covalent bonds, whereas the changes in covalent bonds usually require higher chemical driving forces, resulting in limited sensitivity and reversibility of the probes. The exploration of high-performance pH probes has been a subject of intense investigation. Herein, a new pH probe has been developed, with optical property investigation suggesting the probe has excellent signal-to-noise ratio, and fluorescence intensity shows exponential growth, combined with a visible color change, as pH increased from 5.1 to 6.0; Moreover, the probe has outstanding stability and reversibility, with more than 90% of the initial signal intensity remaining after 30 cycles. In order to better understand the special fluorescence behavior of the reported probe, the non-halogenated isomer is introduced for comparison, combined with the results of structural analysis, quantitative calculation and optical experiments, and the possible mechanism of the special supramolecular aggregation-caused quenching effect induced by the halogen atom is discussed. [ABSTRACT FROM AUTHOR]

Due to the inaccuracy of the preset rolling force of cold rolling, there is a severe thickness defect in the strip head after cold rolling due to the flying gauge change (FGC), which affects the yield of the strip. This paper establishes a rolling force preset model (RFPM) by combining the rolling force optimization model (RFOM) and the rolling force deviation prediction model (RFDPM). The RFOM used a genetic algorithm (GA) to optimize the deformation resistance and friction coefficient models. The RFDPM is constructed using a backpropagation (BP) neural network. The calculation result of the RFPM shows that the average fraction defect of the preset rolling force is only 1.24%, which proves that the RFPM has good calculation accuracy. Experiments show that the defect length proportion of the strip head thickness at less than 20 m after FGC increases from 38.8% to 55.8%, while the average defect length decreases from 47.3 m to 29.6 m, effectively improving the yield of cold rolling. [ABSTRACT FROM AUTHOR]

Plasma electrolytic oxidation (PEO) is widely used as a surface modification method to enhance the corrosion resistance of Mg alloy, the most likely applied biodegradable material used in orthopedic implants. However, the pores and cracks easily formed on the PEO surface are unfavorable for long-term corrosion resistance. In this study, to solve this problem, we used simple immersion processes to construct Mn and Fe oxyhydroxide duplex layers on the PEO-treated AZ31 (PEO–Mn/Fe). As control groups, single Mn and Fe oxyhydroxide layers were also fabricated on PEO (denoted as PEO–Mn and PEO–Fe, respectively). PEO–Mn showed a similar porous morphology to the PEO sample. However, the PEO–Fe and PEO–Mn/Fe films completely sealed the pores on the PEO surfaces, and no cracks were observed even after the samples were immersed in water for 7 days. Compared with PEO, PEO–Mn, and PEO–Fe, PEO–Mn/Fe exhibited a significantly lower self-corrosion current, suggesting better corrosion resistance. In vitro C3H10T1/2 cell culture showed that PEO–Fe/Mn promoted the best cell growth, alkaline phosphatase activity, and bone-related gene expression. Furthermore, the rat femur implantation experiment showed that PEO–Fe/Mn–coated Mg showed the best bone regeneration and osteointegration abilities. Owing to enhanced corrosion resistance and osteogenesis, the PEO–Fe/Mn film on Mg alloy is promising for orthopedic applications. [ABSTRACT FROM AUTHOR]

Simple Summary: Thyroid cancer is the most common endocrine cancer. As tumor-suppressor genes (TSGs) are implicated in many different functions in the organism, their loss in cells in a normal tissue may drive their transformation into cancer cells. TSGs are generally classified into three subclasses: (i) gatekeepers that encode proteins involved in the control of cell cycle and apoptosis; (ii) caretakers that produce proteins implicated in maintaining genomic stability; and (iii) landscapers that, when mutated, create a suitable environment for neoplastic growth. Different inactivation mechanisms may suppress TSG function. Understanding these mechanisms and TSG alterations in thyroid tumors is of great importance for thyroid cancer prognosis, diagnosis, and therapy. The present review paper discusses TSG inactivation mechanisms and alterations in order to help to identify more efficient therapeutic modalities for thyroid cancer management. Thyroid cancer is the most frequent endocrine malignancy and accounts for approximately 1% of all diagnosed cancers. A variety of mechanisms are involved in the transformation of a normal tissue into a malignant one. Loss of tumor-suppressor gene (TSG) function is one of these mechanisms. The normal functions of TSGs include cell proliferation and differentiation control, genomic integrity maintenance, DNA damage repair, and signaling pathway regulation. TSGs are generally classified into three subclasses: (i) gatekeepers that encode proteins involved in cell cycle and apoptosis control; (ii) caretakers that produce proteins implicated in the genomic stability maintenance; and (iii) landscapers that, when mutated, create a suitable environment for malignant cell growth. Several possible mechanisms have been implicated in TSG inactivation. Reviewing the various TSG alteration types detected in thyroid cancers may help researchers to better understand the TSG defects implicated in the development/progression of this cancer type and to find potential targets for prognostic, predictive, diagnostic, and therapeutic purposes. Hence, the main purposes of this review article are to describe the various TSG inactivation mechanisms and alterations in human thyroid cancer, and the current therapeutic options for targeting TSGs in thyroid cancer. [ABSTRACT FROM AUTHOR]

Light-emitting diodes based on colloidal quantum dots (QLEDs) show a good prospect in commercial application due to their narrow spectral linewidths, wide color range, excellent luminance efficiency, and long operating lifetime. However, the toxicity of heavy-metal elements, such as Cd-based QLEDs or Pb-based perovskite QLEDs, with excellent performance, will inevitably pose a serious threat to people's health and the environment. Among heavy-metal-free materials, InP quantum dots (QDs) have been paid special attention, because of their wide emission, which can, in principle, be tuned throughout the whole visible and near-infrared range by changing their size, and InP QDs are generally regarded as one of the most promising materials for heavy-metal-free QLEDs for the next generation displays and solid-state lighting. In this review, the great progress of QLEDs, based on the fundamental structure and photophysical properties of InP QDs, is illustrated systematically. In addition, the remarkable achievements of QLEDs, based on their modification of materials, such as ligands exchange of InP QDs, and the optimization of the charge transport layer, are summarized. Finally, an outlook is shown about the challenge faced by QLED, as well as possible pathway to enhancing the device performance. This review provides an overview of the recent developments of InP QLED applications and outlines the challenges for achieving the high-performance devices. [ABSTRACT FROM AUTHOR]

Graphene oxide-cysteamine-silver nanoparticle (GCA)/silver nanowire (AgNW)/GCA/colorless poly(amide-imide) (cPAI) structures based on cPAI substrates with polyimide and polyamide syntheses were fabricated to study their characteristics. A layer of electrodes was constructed using a sandwich structure—such as GCA/AgNW/GCA—with cPAI used as a substrate to increase the heat resistance and improve their mechanical properties. Furthermore, to overcome the disadvantages of AgNWs—such as their high surface roughness and weak adhesion between the substrate and electrode layers—electrodes with embedded structures were fabricated using a peel-off process. Through bending, tapping, and durability tests, it was confirmed that these multilayer electrodes exhibited better mechanical durability than conventional AgNW electrodes. Resistive random-access memory based on GCA/AgNW/GCA/cPAI electrodes was fabricated, and its applicability to nonvolatile memory was confirmed. The memory device had an ON/OFF current ratio of ~104@0.5 V, exhibiting write-once-read-many time characteristics, maintaining these memory characteristics for up to 300 sweep cycles. These findings suggest that GCA/AgNW/GCA/cPAI electrodes could be used as flexible and transparent electrodes for next-generation flexible nonvolatile memories. [ABSTRACT FROM AUTHOR]

This paper aims to study the relationship between the microstructure and the mechanical properties of X70 submarine pipeline steel with 40.5 mm thickness. The microstructure was examined by using optical microscopy, scanning electron microscopy and an electron backscattered diffractometer, while the mechanical properties were examined by using a hardness test, a tensile test, a Charpy impact test and a drop weight tear test (DWTT), respectively. The results show that the base metal (BM) of the pipe has a low yield ratio of 0.83 and an excellent elongation of more than 45%. The DWTT shear area of the steel plate reaches 87%, showing excellent low-temperature toughness. The Charpy impact energy increases when the distance from the fusion line increases, and it reaches a maximum at the BM near the heat-affected zone (HAZ) due to the small martensite-austenite (MA) constituents and fine grains. The concentrated distribution of blocky/slender MA constituents along the prior austenite grain boundaries of the intercritically reheated coarse-grained HAZ and the large MA constituents are the main reasons for the deteriorating impact toughness. Delamination cracks in the DWTT fracture surface only occurred in the midthickness of a sample with a small opening width that spread about 2.1 mm perpendicular to the DWTT fracture surface and were finally arrested at the acicular ferrite clusters containing a high density of high-angle boundaries. [ABSTRACT FROM AUTHOR]

The online preparation of fibers using molten modified blast furnace slag can not only achieve the high-value-added utilization of the slag but can also make use of the sensible heat of the slag. In this paper, blast furnace slag was modified using iron tailings, and was then used to prepare slag fiber online; the effects of the acidity coefficient on the properties of the molten modified blast furnace slag and modified blast furnace slag fiber were investigated. With an increase in the acidity coefficient from 1.2 to 1.6, the temperature range of the slag melt, with viscosity in the 1–3 Pa·s range, increased from 101.2 °C to 119.9 °C. The melting temperature increased from 1326.2 °C to 1388.7 °C, and the suitable fiber-forming temperature range increased from 70.7 °C to 82.9 °C. With the increasing acidity coefficient, the crystallization temperature of the molten modified slag decreased markedly. When the acidity coefficient was greater than 1.4, the slag system was still in a disordered glassy phase at 1100 °C. The hardening speed gradually reduced with the increasing acidity coefficient when the modified slag was cooled at the critical cooling rate, resulting in a gradual increase in fiber formability. The fibers prepared from the modified slag at different acidity coefficients had smooth surfaces, and were arranged in a crossed manner at the macroscopic level. Their color was white, and small quantities of slag balls were doped inside the fibers. With an increase in the acidity coefficient from 1.2 to 1.6, the average fiber diameter increased from 4.2 μm to 8.2 μm, and their slag ball content increased from 0.73% to 4.49%. Overall, the acidity coefficient of modified blast furnace slag should be less than 1.5 in actual production. [ABSTRACT FROM AUTHOR]

Vascular endothelial growth factor A (VEGF-A) is a secreted protein that stimulates angiogenesis in response to hypoxia. Under hypoxic conditions, a non-canonical long isoform called L-VEGF is concomitantly expressed with VEGF-A. Once translated, L-VEGF is proteolytically cleaved to generate N-VEGF and VEGF-A. Interestingly, while VEGF-A is secreted and affects the surrounding cells, N-VEGF is mobilized to the nucleus. This suggests that N-VEGF participates in transcriptional response to hypoxia. In this study, we performed a series of complementary experiments to examine the functional role of N-VEGF. Strikingly, we found that the mere expression of N-VEGF followed by its hypoxia-independent mobilization to the nucleus was sufficient to induce key genes associated with angiogenesis, such as Hif1α,VEGF-A isoforms, as well as genes associated with cell survival under hypoxia. Complementarily, when N-VEGF was genetically depleted, key hypoxia-induced genes were downregulated and cells were significantly susceptible to hypoxia-mediated apoptosis. This is the first report of N-VEGF serving as an autoregulatory arm of VEGF-A. Further experiments will be needed to determine the role of N-VEGF in cancer and embryogenesis. [ABSTRACT FROM AUTHOR]

A high-strength low-carbon construction structural steel was investigated in the laboratory. The various austenite grain sizes were obtained by austenitizing the steel at different temperatures. The effect of austenite grain size on bainite transformation was studied by the dilatometer. The results show that the microstructure of high-strength low-carbon structural steels mainly includes granular bainite, lath-like bainite and martensite-austenite (M-A). The microstructure changes from granular bainite to lath-like bainite with the increase in austenitizing temperature or austenite grain size. When the samples were heated at the lower temperature of 860 °C, the bainite starting temperature was relatively high, which was mainly attributed to the promotion of the granular bainitic nucleation and the formation of the solute-depleted regions in the austenite. Compared to 860 and 1260 °C, the bainite transformation rate in the specimen austenitized at 1000 °C is the highest because of the small prior austenite grain size and larger transformation driving force. [ABSTRACT FROM AUTHOR]

Identifying users across social media has practical applications in many research areas, such as user behavior prediction, commercial recommendation systems, and information retrieval. In this paper, we propose a multiple salient features-based user identification across social media (MSF-UI), which extracts and fuses the rich redundant features contained in user display name, network topology, and published content. According to the differences between users' different features, a multi-module calculation method is used to obtain the similarity between various redundant features. Finally, the bidirectional stable marriage matching algorithm is used for user identification across social media. Experimental results show that: (1) Compared with single-attribute features, the multi-dimensional information generated by users is integrated to optimize the universality of user identification; (2) Compared with baseline methods such as ranking-based cross-matching (RCM) and random forest confirmation algorithm based on stable marriage matching (RFCA-SMM), this method can effectively improve precision rate, recall rate, and comprehensive evaluation index (F1). [ABSTRACT FROM AUTHOR]

In this article, we implement and compare 10 node removal (attack) strategies from the literature over the photosystem I (PSI) complex network of the common pea plant (Pisum sativum), representing the FRET energy transfer among its nodes/chromophores. We measure the network robustness (functioning) with four indicators. The node attack strategies and the network robustness indicators consider both the binary-topological and the weighted structure of the network. First, we find that the well-known node betweenness centrality attack, which has proven highly effective in dismantling most real-world networks' topological connectivity, is ineffective over the PSI network. Second, the degeneracy of the node properties caused by the PSI's higher network connectivity level induces a random-like node removal even when nodes are removed according to a specific node centrality measure. This phenomenon triggers a very low decrease of the PSI network functioning even when subjected to node attack. Such an outcome would indicate that the node attack strategies based on classic node properties, such as the degree or the betweenness centrality, may show low efficacy in dismantling real-world networks with very high connectivity levels. Last, the PSI network can be built by tuning a cut-off distance (CD) that defines the viable energy transfers among nodes/chromophores and progressively discards the lower energy transfer links among distant nodes/chromophores. This represents a 'weight thresholding' procedure allowing us to investigate the efficacy of the node attack strategies when links of lower weight are progressively pruned from the PSI network. We find that the best node attack strategies change by decreasing the CD, showing that the weight thresholding procedure affects the network response to node removal. This last outcome outlines the importance of investigating the stability of the system response for real-world weighted complex networks subjected to the weight thresholding procedure. [ABSTRACT FROM AUTHOR]

The very fast evolution in wearable electronics drives the need for energy storage micro-devices, which have to be flexible. Micro-supercapacitors are of high interest because of their high power density, long cycle lifetime and fast charge and discharge. Recent developments on micro-supercapacitors focus on improving the energy density, overall electrochemical performance, and mechanical properties. In this review, the different types of micro-supercapacitors and configurations are briefly introduced. Then, the advances in carbon electrode materials are presented, including activated carbon, carbon nanotubes, graphene, onion-like carbon, and carbide-derived carbon. The different types of electrolytes used in studies on micro-supercapacitors are also treated, including aqueous, organic, ionic liquid, solid-state, and quasi-solid-state electrolytes. Furthermore, the latest developments in fabrication techniques for micro-supercapacitors, such as different deposition, coating, etching, and printing technologies, are discussed in this review on carbon electrode-based micro-supercapacitors. [ABSTRACT FROM AUTHOR]

Motivation Tea is a cross-pollinated woody perennial plant, which is why, application of conventional breeding is limited for its genetic improvement. However, lack of the genome-wide high-density SNP markers and genome-wide haplotype information has greatly hampered the utilization of tea genetic resources toward fast-track tea breeding programs. To address this challenge, we have generated a first-generation haplotype map of tea (Tea HapMap-1). Out-crossing and highly heterozygous nature of tea plants, make them more complicated for DNA-level variant discovery. Results In this study, whole genome re-sequencing data of 369 tea genotypes were used to generate 2,334,564 biallelic SNPs and 1,447,985 InDels. Around 2928.04 million paired-end reads were used with an average mapping depth of ∼0.31× per accession. Identified polymorphic sites in this study will be useful in mapping the genomic regions responsible for important traits of tea. These resources lay the foundation for future research to understand the genetic diversity within tea germplasm and utilize genes that determine tea quality. This will further facilitate the understanding of tea genome evolution and tea metabolite pathways thus, offers an effective germplasm utilization for breeding the tea varieties. Supplementary information Supplementary data are available at Bioinformatics online. [ABSTRACT FROM AUTHOR]

In the present paper, a novel method was developed to predict the dynamic recrystallization (DRX) behavior during hot rolling based on machine learning in combination with physical models of niobium (Nb) micro-alloyed steels. It has been demonstrated that the comprehensive modeling could accurately predict the DRX behavior under different compositional and processing conditions, which not only exhibited higher precisions but also was in better agreement with physical metallurgical principles than traditional methods. [ABSTRACT FROM AUTHOR]

To build high-performance flexible electronic devices, carbon based nanomaterials can be used as next-generation electronic materials due to their excellent properties, including high carrier mobility, excellent mechanical flexibility, high current-carrying capacity, and low-cost production. It is worth noting that the ultrathin shape of carbon-based nanomaterials can eliminate the mechanical mismatch between organs or tissues and electronic devices, which can facilitate the development of bioelectronics [[19]]. With the rapid development of society, the growing interest in flexible electronics has led to remarkable progress in recent advances in the manufacture of flexible electronics. Carbon nanotubes and graphene, as representatives of one-dimensional and two-dimensional carbon-based nanomaterials, not only have similar physical structures but also, more importantly, have excellent mechanical properties [[16]]. [Extracted from the article]

The effects of die radius in a streamlined die on design factors, such as the distribution of strain, stress, temperature, damage, and drawing force of a wire, were investigated during wire drawing for a better understanding of streamlined die and improvement in drawing quality of the wire. A numerical simulation was performed with the die radius of the streamlined die. The behavior of the design factors of the drawn wire fabricated by the streamlined die was different from that of the conventional die and was highly dependent on the die radius. The different behaviors of the design factors with the die radius can be explained by the frictional work and redundant work of the wire with die angle. The temperature rise and drawing force were high at a greater die radius because of the great frictional stress and heating effect stemming from the high contact length of the wire and die. Meanwhile, the higher redundant work at the surface area with decreasing die radius led to higher strain inhomogeneity, effective stress, damage value, temperature rise, and drawing force due to the abrupt change in the metal flow of the wire stemming from the high die angle. After the optimization of several design factors with die radius, it was concluded that the optimum IDR values ranged from 0.14 to 0.18, indicating that a streamlined die with a radius of 70 to 90 mm was the most suitable in the present process condition. In particular, the damage value of the wire was reduced in this range of die radii compared to the conventional die. [ABSTRACT FROM AUTHOR]

To obtain the correlation of microstructural characteristics and toughness in a novel high-strength low-carbon bainitic structural steel with medium and heavy plate after multipass welding, a welding thermal simulation experiment was conducted to simulate different subregions in the reheated coarse-grained heat-affected zones (CGHAZ). The microstructure evolution was then analyzed and factors that influence the fracture behavior were studied. The results show that the brittle zone appeared in subcritical reheated CGHAZ, and the fractured morphology was cleavage fracture. Supercritical reheated CGHAZ had the highest impact toughness, and the fractured morphology was primarily the ductile fracture with dimples formed via the micropore polycondensation mechanism. With an increase in the secondary pass welding thermal cycle peak temperature (tp2), the average length size of martensite and austenite (M-A) decreased from 9 to 2 μm. The coarsening of M-A constituents was the main reason for decrease in the crack initiation absorbed energy. A large number of retained austenite and cementite precipitates in subcritical reheated CGHAZ clearly worsened the impact toughness, and the massive austenite and cementite precipitates more than offset the beneficial effects of high-angle boundaries. This phenomenon led to disappearance of the effect of high-angle grain boundary of prior austenite and lath bainite on arresting crack propagation. In supercritical reheated CGHAZ, crack propagation absorbed energy was increased because of grain refinement, fine precipitates, lamellar residual austenite at corners, and high-angle grain boundary. [ABSTRACT FROM AUTHOR]

With the rapid development of rail vehicles, 301L strip has become the main material of the rail vehicles, and its flatness quality has got much attention. Meanwhile, 20-roll mill becomes the most important cold rolling equipment for 301L strip forming, because of its high precision and wide application. To study the flatness control theory of cold rolling 301L strip in 20-roll mill, a finite element model is established based on elastoplastic theory. The stress–strain field is used to characterize the strip flatness under different rolling parameters. After analysis, the results show that the front and back tension have the most obvious effects on strip flatness. The friction coefficient and rolling speed are beneficial to the control of the strip flatness when they increase within a certain range. [ABSTRACT FROM AUTHOR]

(1) Background: Osteonecrosis (ON) of the femoral head is a disabling disease for which limited treatment options exist. Identifying therapeutic targets of its evolution could provide crucial insights into multi-targeted approaches. The aim of this pilot study was to assess the histopathological features of patients with non-traumatic femoral head (NTFH) and post-traumatic femoral head (PTFH) ON to produce a fresh vision for clinical use. (2) Methods: We got biopsies from patients with different ON stages, according to the ARCO system. Samples from multi-organ donors were used as controls. Histological and immunohistochemical evaluations were performed on the osteochondral unit. (3) Results: The PTFH group displayed several fibrotic reactions, a small stem cell pool and a lower international cartilage repair society (ICRS)-I score than NTFH, which instead presented intact cartilage similar to the controls. Immunostaining for collagen I and autotaxin confirmed these features in the PTFH group, which displayed top levels of MMP-13 involved in cartilage loss and reduced CB-2 in the underlying bone. Both groups manifested a similar pattern of apoptotic and pain mediators. (4) Conclusions: The different histopathological features suggest a multi-disciplinary and multi-targeted approach for ON. Further studies are necessary to measure the effect size to gain clinical evidence. [ABSTRACT FROM AUTHOR]

Purpose: This paper aims to study the effect of current density of hydrogen charging on the semiconductor properties and pitting initiation of 2205 duplex stainless steel (DSS) passivation film. Design/methodology/approach: In this work, the 2205 DSS is pre-hydrogenated and passivated. Then, the passivation film is tested by electrochemical impedance method, Mott–Schottky curve method and dynamic potential scanning method. The influences of hydrogen on the properties of the passivation film and the corrosion behavior of the matrix were studied by analyzing the curves obtained in the electrochemical test. The surface of the passivation film after pre-hydrogenation and anodic polarization was observed by using the ultra-depth three-dimensional microscopy and the scanning electron microscope. The integrity, density and corrosion morphology of the passivation film were studied and discussed. Findings: With the increase of the hydrogen current density, the growth of the passivation film is hindered, the concentrations of donor and acceptor in the film are increased, the conductivity of the passivation film increases. In the anodic polarization, the dimensional passive current density increases with the increase of the hydrogen current density, and the pitting potential is reversed, the more likely the sample is pitting. In general, hydrogen hinders the formation of the passive film on duplex stainless steel, which increases the concentration of point defects in the passive film. Finally, the passive film is easy to crack and pitting. Originality/value: The performance of passive film is an important condition to influence the corrosion behavior of stainless steel. However, little research has been done on the effects of hydrogen on the electrochemistry and pitting sensitivity of 2205 DSS passivation films. The effect of hydrogen on semiconductor properties and pitting initiation of 2205 DSS passivation film is needed to be investigated. [ABSTRACT FROM AUTHOR]

Bone as such displays an intrinsic regenerative potential following fracture; however, this capacity is limited with large bone defects that cannot heal spontaneously. The management of critical‐sized bone defects remains a major clinical and socioeconomic need with osteoregenerative biomaterials constantly under development aiming at promoting and enhancing bone healing. X‐ray computed tomography (XCT) has become a standard and essential tool for quantifying structure–function relationships in bone and biomaterials, facilitating the development of novel bone tissue engineering strategies. This paper presents recent advancements in XCT analysis of biomaterial‐mediated bone regeneration. As a noninvasive and nondestructive technique, XCT allows for qualitative and quantitative evaluation of three‐dimensional (3D) scaffolds and biomaterial microarchitecture, bone growth into the scaffold as well as the 3D characterisation of biomaterial degradation and bone regeneration in vitro and in vivo. Furthermore, in combination with in situ mechanical testing and digital volume correlation (DVC), XCT demonstrated its potential to better understand the bone–biomaterial interactions and local mechanics of bone regeneration during the healing process in relation to the regeneration achieved in vivo, which will likely provide valuable knowledge for the development and optimisation of novel osteoregenerative biomaterials. Lay Description: Bone, being a dynamically adaptable material, displays excellent regenerative properties following fracture. However, the self‐healing capacity of bone becomes more difficult with large bone defects. Those defects are common and occur in many clinical situations; hence, biomaterials are mostly used to restore both bone structure and function in the defect site. X‐ray computed tomography (XCT) is a powerful tool to evaluate bone regeneration in critical‐sized defects after the implantation of biomaterials, allowing to an improved understanding of the regeneration process following different bone tissue engineering approaches. This paper focuses on recent advancements in XCT analysis to characterise biomaterial‐mediated bone regeneration in critical‐sized defects. XCT supports three‐dimensional (3D) analysis of biomaterials, scaffolds and regenerated bone microarchitecture, as well as bone ingrowth into the scaffold. As a nondestructive technique, XCT allows for a 3D characterisation of biomaterial degradation and bone regeneration over time. In addition, XCT combined with in situ mechanical experiments and digital volume correlation (DVC) provides a 3D evaluation and quantification of bone–biomaterial interactions and deformation mechanisms during the regeneration process. This remains essential for the development and enhancement of novel biomaterials able to produce bone that is comparable with the native tissue they aim to replace. [ABSTRACT FROM AUTHOR]

We investigated structural changes, phase diagram, and vibrational properties of hydrogen hydrate in filled-ice phase C2 by using first principles molecular dynamics simulation. It was found that the experimentally reported 'cubic' structure is unstable at low temperature and/or high pressure: The 'cubic' structure reflects the symmetry at high (room) temperature where the hydrogen bond network is disordered and the hydrogen molecules are orientationally disordered due to thermal rotation. In this sense, the 'cubic' symmetry would definitely be lowered at low temperature where the hydrogen bond network and the hydrogen molecules are expected to be ordered. At room temperature and below 30 GPa, it is the thermal effects that play an essential role in stabilizing the structure in 'cubic' symmetry. Above 60 GPa, the hydrogen bonds in the framework would be symmetrized and the hydrogen bond order-disorder transition would disappear. These results also suggest the phase behavior of other filled-ice hydrates. In the case of rare gas hydrate, there would be no guest molecules' rotation-nonrotation transition since the guest molecules keep their spherical symmetry at any temperature. On the contrary methane hydrate MH-III would show complex transitions due to the lower symmetry of the guest molecule. These results would encourage further experimental studies, especially nuclear magnetic resonance spectroscopy and neutron scattering, on the phases of filled-ice hydrates at high pressures and/or low temperatures. [ABSTRACT FROM AUTHOR]

Porous materials are promising media for designing medical instruments, drug carriers, and bioimplants because of their excellent biocompatibility, ease of design, and large variation of elastic moduli. In this study, a computational strategy using the finite element method is developed to model the porous microstructures and to predict the relevant elastic moduli considering the actual characteristics of the micropores and their distributions. First, an element-based approach is presented to generate pores of different shapes and sizes according to the experimental observations. Then, a computational scheme to evaluate the effective moduli of macroscopically isotropic porous materials based on their micro-mechanics is introduced. Next, the accuracy of our approach is verified with the analytical solutions of the extreme bounds of the elastic isotropic moduli of a simplified model and with the experimental data available in the literature. Finally, the influence of the shape of pores and their distribution modes are assessed. [ABSTRACT FROM AUTHOR]

The subject of human-hand versatility has been intensively investigated for many years. Emerging robotic constructions change continuously in order to mimic natural mechanisms as accurately as possible. Such an attitude is motivated by the demand for humanoid robots with sophisticated end effectors and highly biomimic prostheses. This paper provides wide analysis of more than 80 devices that have been created over the last 40 years. It compares both the mechanical structure and various actuators from conventional DC motors and servomechanisms, through pneumatic muscles, to soft actuators and artificial muscles. Described measured factors include angles, forces, torques, tensions, and tactiles. Furthermore, the appropriate statistics of kinematic configuration, as well as the type or number of drive units and sensory systems, show not only recent problems, but also trends that will be followed in the future. [ABSTRACT FROM AUTHOR]

Multiple gene polymorphisms have been demonstrated to correlate with the susceptibility to osteonecrosis of the femoral head (ONFH). However, as a complex disease induced by multiple genes, the development of ONFH has rarely been reported to involve in gene interaction. In this study, we first explored the association of 10 variants interactions in receptor activator of nuclear factor-kappa B (RANK), RANK ligand (RANKL), osteoprotegerin (OPG), tumor necrosis factor receptor-associated factor 6 (TRAF6), and nuclear factor of activated T cells cytoplasmic 1 (NFATC1) genes with the development and clinical phenotypes of ONFH in a 377 ONFH case–control study with using Mass ARRAY® platform. Our results showed that not only a total of 6 interactional variants in the paired 10 variants interactions were significantly associated with the development of ONFH (OPG rs2073617 and NFATC1 rs754093, p < 0.019; OPG rs2073618 and NFATC1 rs754093, p < 0.008; OPG rs2073617 and RANKL rs1054016, p < 0.039, respectively) but also a total of 4 paired interactional variants were found to involve significantly in the increased risk of bilateral hip lesions in ONFH (OPG rs2073617 and TRAF6 rs5030411, p = 0.044; RANK rs884205 and TRAF6 rs5030411, p = 0.045, respectively). Moreover, the results from generalized multifactor dimensionality reduction also showed that the five best models were identified and associated significantly with ONFH risk, p = 0.001, 0.01, 0.01, 0.01, and 0.01, respectively. Our results first suggest that the variants in RANK/RANKL/OPG pathway genes affected the development of ONFH in gene interaction manner through the interaction of the paired variants and multiple variants. [ABSTRACT FROM AUTHOR]

The voltage on the valve side windings of the converter transformer is non-sinusoidal, which contains many high-order harmonic components. To calculate the nonlinear electric field distribution under the non-sinusoidal voltage, the harmonic-balanced decomposition finite element method (HBDFEM) is proposed in this paper. By using the fixed-point method, the finite element stiffness matrix of each iteration is decomposed according to the harmonic order, by which the memory consumption can be remarkably reduced. To verify the effectiveness of the proposed algorithm, a typical model of the oil-paper insulation structure is taken for instance, and the results obtained by the HBDFEM is compared with two algorithms, the traditional HBFEM and the time step method. The calculation results show that the HBDFEM can effectively reduce the computation cost for the nonlinear electric field problems with the non-sinusoidal periodic voltage. And it can be applied to analyze the compound electric field problems of the actual converter transformer. The electric field distribution of a ±500 kV converter transformer is calculated and analyzed considering the nonlinear relationship between the electric field and the medium conductivity. [ABSTRACT FROM AUTHOR]

A facile method for the assembly of polyaniline/graphene oxide (PANi/GO) composite hydrogel has been successfully demonstrated by in‐situ polymerization of aniline in the presence of phytic acid with the aid of GO. During the polymerization of aniline, the 2D GO nanosheets can regulate the aggregation state of polymer and prompt the formation of the hydrogel. The chemical structure, morphology and mechanical property are investigated by Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy and compressive stress–strain measurements, et al. Pani/GO composite hydrogel with high surface area and 3D porous nanostructures exhibits higher adsorption capability for Cr(VI) in compared with PANi hydrogel. Pseudo‐second‐order and Langmuir isotherm models are effectively employed to describe the adsorption behaviors of PANi/GO composite hydrogel. Furthermore, the regeneration experiments indicate that the as‐prepared composite hydrogel can remain 73.5% of the original removal capability after five adsorption–desorption cycles. It is expected that the PANi/GO composite hydrogel can be an effective candidate for the removal of Cr(VI) due to the synergistic effects between PANi and GO within the hydrogel network. POLYM. COMPOS., 40:E1777–E1785, 2019. © 2019 Society of Plastics Engineers [ABSTRACT FROM AUTHOR]

To precisely control the characteristics of nonmetallic inclusions with high plasticity in U71Mnk high-speed heavy-rail steel deoxidized by Si and Mn, the chemical composition of CaO-SiO2-Al2O3-MgO slag in the ladle furnace (LF) refining process was optimized by thermodynamic calculations. Relationships among the refining slag, nonmetallic inclusions, and elemental concentrations in the steels were analyzed to obtain the conditions for determining the optimal composition of refining slag. The optimal compositions and the component activities of CaO-SiO2-Al2O3-MgO refining slag were determined and discussed according to calculations using FactSage 7.0. Finally, ten industrial experiments were performed. Variations in the compositions of LF refining slag, inclusions, and steel, as well as the sizes of the inclusions, and metallographic classification assessment of the U71Mnk steel product, were investigated and discussed to verify the feasibility and effectiveness of the optimized LF refining slag. Kinetics of steel-slag and steel-inclusion reactions during LF refining was studied and compared. The results show that to obtain low [O], [Al], and [S] contents and inclusions with high plasticity in molten steel, the optimal composition of the LF refining slag should be CaO: 44.2 to 51.7 wt pct, SiO2: 41.4 to 47.0 wt pct, Al2O3: 0 to 4.7 wt pct and MgO 5.0 to 7.0 wt pct, where the basicity R is above 1.00 and the C/A ratio is above 9.00. After the LF refining process, the contents of total oxygen (T.O.), [Al], and [S] gradually stabilized in the range of 0.0008 to 0.0012 wt pct, 0.002 to 0.0028 wt pct and 0.0026 to 0.0037 wt pct, respectively. The densities of the three kinds of typical inclusions (CaO-SiO2-Al2O3-MgO, MnS, and CaO-SiO2-Al2O3-MgO-MnS) also decreased and reached approximately 0.25/mm2, and no inclusion larger than 6.0 μm was found. The composition of the inclusions gradually changed into the optimal range in the CaO-SiO2-Al2O3-MgO slag system during the LF refining process. [ABSTRACT FROM AUTHOR]

Featured Application: This work will serve as a valuable resource for the research on APS. The Astragalus polysaccharides (APS) are important bioactive components of Astragali Radix, the dry root of Astragalus membranaceus, which has been used in traditional Chinese medicine. In this review, the extraction conditions and extraction rates of APS are first compared for water, microwave-assisted, ultrasonic wave, and enzymatic hydrolysis extraction methods. Some studies have also shown that different methods can be combined to improve the extraction rate of APS. Subsequently, the chemical composition and structure of APS are discussed, as related to the extraction and purification method. Most studies have shown that APS is mainly composed of glucose, in addition to rhamnose, galactose, arabinose, xylose, mannose, glucuronic acid, and galacturonic acid. We also reviewed studies on the modification of APS using chemical methods, including sulfated modification using the chlorosulfonic acid–pyridine method, which is commonly used for chemical modification of APS. Finally, the pharmacological activities and mechanisms of action of APS are summarized, with a special focus on its immunoregulatory, antitumor, anti-inflammatory, and antiviral effects. This review will serve as a valuable resource for the research on APS. [ABSTRACT FROM AUTHOR]

The formation mechanism of nanoscale Al3Fe phase in Al-1Fe (wt pct) alloy during rheo-extrusion was investigated, and the mechanical property of the prepared alloy was also measured. The results show that the average length of Al3Fe phase in Al-1Fe alloy prepared by rheo-extrusion is 300 nm, which is much more refined than the needlelike Al3Fe phase in as-cast Al-1Fe alloy (50 μm). In rheo-extrusion, Al3Fe phase formed by eutectic reaction is bonelike, but it could be continuously refined by the shear deformation in the wheel groove, in equal channel angular flow, and in expansion extrusion mold. The total equivalent strain of the shear deformation is higher than 4.82. The tensile strength and elongation of Al-1Fe alloy prepared by rheo-extrusion are 135 MPa and 30 pct, respectively. The tensile strength of Al-1Fe alloy prepared by rheo-extrusion is 58.8 pct higher than that of as-cast Al-1Fe alloy, and the elongation is 19 pct higher than that of as-cast Al-1Fe alloy. Compared with as-cast Al-1Fe alloy, the improvements of tensile strength and elongation caused by shear deformation in rheo-extrusion are higher than the reported improvements induced by rare earth modification. [ABSTRACT FROM AUTHOR]

Three experimental hole transport materials containing fluorine-substituted benzothiadiazole-based organic molecules (Jy5–Jy7) have been studied to explore the relationship between photoelectric performances and the core structures of hole transport materials (HTM). By employing density functional theory (DFT) and time-dependent density functional theory (TD-DFT), it was found that the substitution of the hydrogen atom by fluorine atom in the core structure can significantly boost the hole mobility; and the replacement of core structure from electron-withdrawing group to electron-donating group has strong influence on the increment of LUMO level energy, ability to preventing electron-backflow, molecular stability and oscillator strength of HTM molecules. We hope our investigation can provide theoretical guidance to reasonably optimize HTM molecules for perovskite solar cells. [ABSTRACT FROM AUTHOR]

Purpose The purpose of this paper is to examine the effect of adopting certified food production on chemical fertilizer and pesticide use in China.Design/methodology/approach The authors estimate fixed effect models to track the changes in agro chemical consumption at household level over time and evaluate the effect of certified food production, using an unbalanced panel data set covering 4,830 households in six provinces over the period 2005–2013.Findings On average, the authors do not find significant effects of certified food production on either chemical fertilizer or pesticide consumption among Chinese farmers. The effects are heterogeneous across villages, but the heterogeneous effects show no clear pattern that is consistent with different types of certification. The findings are robust to the use of alternative panel structure and certification indicators. The lack of knowledge about certification among farmers, the price premium and differences in regulation enforcement across regions may explain why the authors do not find negative effects on agro chemical use.Practical implications This study suggests that careful inspections and strong enforcement of certified food production is needed to ensure that the environmental goals of certified food production can be achieved and the reputation of certification in China can be improved. The inspection of certification producers and the enforcement of current regulations should be stricter for the further healthy development of certified food production in China.Originality/value This study is the first attempt to systematically evaluate the impact of food certification on the use of agro chemicals in Chinese agriculture. [ABSTRACT FROM AUTHOR]

In this investigation, a thermal welding simulation technique was used to investigate the microstructures and mechanical properties of the intercritically reheated coarse grain heat-affected zone (IR CGHAZ) of ASTM4130 steel. The effect of post weld heat treatment (PWHT) on the toughness of IR CGHAZ was also analyzed. The toughness of IR CGHAZ was measured by means of Charpy impact, and it is found that IR CGHAZ has the lowest toughness which is much lower than that of the base metal regardless of whether PWHT is applied or not. The as-welded IR CGHAZ is mainly composed of ferrite, martensite, and many blocky M-A constituents distributing along grain boundaries and subgrain boundaries in a near-connected network. Also, the prior austenite grains are still as coarse as those in the coarse grain heat-affected zone (CGHAZ). The presence of the blocky M-A constituents and the coarsened austenite grains result in the toughness deterioration of the as-welded IR CGHAZ. Most of the blocky M-A constituents are decomposed to granular bainite due to the effect of the PWHT. However, PWHT cannot refine the prior austenite grains. Thus, the low toughness of IR CGHAZ after PWHT can be attributed to two factors, i.e., the coarsened austenite grains, and the presence of the remaining M-A constituents and granular bainite, which are located at grain boundaries and subgrain boundaries in a near-connected network. The absorbed energy of the IR CGHAZ was increased by about 3.75 times, which means that the PWHT can effectively improve the toughness but it cannot be recovered to the level of base metal. [ABSTRACT FROM AUTHOR]

In this study, the performance and stability of inverted bulk heterojunction (BHJ) polymer solar cells (PSCs) is enhanced by doping zinc oxide (ZnO) with 0-6 wt % cetyltrimethylammonium bromide (CTAB) in the sol-gel ZnO precursor solution. The power conversion efficiency (PCE) of the optimized 3 wt % CTAB-doped ZnO PSCs was increased by 9.07%, compared to a PCE of 7.31% for the pristine ZnO device. The 0-6 wt % CTAB-doped ZnO surface roughness was reduced from 2.6 to 1 nm and the number of surface defects decreased. The X-ray photoelectron spectroscopy binding energies of Zn 2p3/2 (1021.92 eV) and 2p1/2 (1044.99 eV) shifted to 1022.83 and 1045.88 eV, respectively, which is related to strong chemical bonding via bromide ions (Br-) that occupy oxygen vacancies in the ZnO lattice, improving the PCE of PSCs. The concentration of CTAB in ZnO significantly affected the work function of PSC devices; however, excessive CTAB increased the work function of the ZnO layer, resulting from the aggregation of CTAB molecules. In addition, after a 120-hour stability test in the atmosphere with 40% relative humidity, the inverted device based on CTAB-doped ZnO retained 92% of its original PCE and that based on pristine ZnO retained 68% of its original PCE. The obtained results demonstrate that the addition of CTAB into ZnO can dramatically influence the optical, electrical, and morphological properties of ZnO, enhancing the performance and stability of BHJ PSCs. [ABSTRACT FROM AUTHOR]

Purpose The purpose of this paper is to present state-of-the-art approaches for precise operation of a robotic manipulator on a macro- to micro/nanoscale.Design/methodology/approach This paper first briefly discussed fundamental issues associated with precise operation of a robotic manipulator on a macro- to micro/nanoscale. Second, this paper described and compared the characteristics of basic components (i.e. mechanical parts, actuators, sensors and control algorithm) of the robotic manipulator. Specifically, commonly used mechanisms of the manipulator were classified and analyzed. In addition, intuitive meaning and applications of its actuator explained and compared in details. Moreover, related research studies on general control algorithm and visual control that are used in a robotic manipulator to achieve precise operation have also been discussed.Findings Remarkable achievements in dexterous mechanical design, excellent actuators, accurate perception, optimized control algorithms, etc., have been made in precise operations of a robotic manipulator. Precise operation is critical for dealing with objects which need to be manufactured, modified and assembled. The operational accuracy is directly affected by the performance of mechanical design, actuators, sensors and control algorithms. Therefore, this paper provides a categorization showing the fundamental concepts and applications of these characteristics.Originality/value This paper presents a categorization of the mechanical design, actuators, sensors and control algorithms of robotic manipulators in the macro- to micro/nanofield for precise operation. [ABSTRACT FROM AUTHOR]

Regulation of crucial lnc RNAs involved in differentiation of chicken embryonic stem cells ( ESCs) to spermatogonia stem cells ( SSCs) was explored by sequencing the transcriptome of ESCs, primordial germ cells ( PGCs) and SSCs with RNA-Seq; analytical bioinformatic methods were used to excavate candidate lnc RNAs. We detected expression of candidate lnc RNAs in ESCs, PGCs and SSCs and forecasted related target genes. Utilizing wego, david and string, function and protein-protein interactions of target genes were analyzed. Finally, based on string analysis, interaction diagrams and relevant signaling pathways were established. Our results indicate a total of 9657 lnc RNAs in ESCs, PGCs and SSCs, with 3549 defined as significantly different. We screened 20 candidate lnc RNAs, each demonstrating a greater than eight-fold difference in |log FC| value between groups ( ESCs vs. PGCs, ESCs vs. SSCs and PGCs vs. SSCs) or specifically expressed in an individual cell type. qRT- PCR results indicated that expression tendencies of candidate lnc RNAs were consistent with RNA-Seq. Fifteen cis and four trans target genes were forecasted. Based on wego and string analyses, we found lnc-SSC1, lnc-SSC5, lnc-SSC2 and lnc- ESC2 negatively regulated target genes SUFU, EPHA3, KLF3, ARL3 and TRIM8, whereas SHH, NOTCH, TGF- β, cAMP/ cGMP and JAK/ STAT signaling pathways were promoted, causing differentiation of ESCs into SSCs. Our findings represent a preliminary unveiling of lnc RNA-associated regulatory mechanisms during differentiation of chicken ESCs into SSCs, filling a research void in male germ cell differentiation related to lnc RNA. Our results also provide basic information for improving in vitro induction systems for differentiation of chicken ESCs into SSCs. [ABSTRACT FROM AUTHOR]

Vanadium pentoxide V2O5 thin films were grown at room temperature on ITO coated glass substrates by electrochemical deposition. The resulting films were annealed at 300, 400 and 500°C for 1 h in ambient environment. The effect of heat treatment on the films properties such as surface morphology, crystal structure, optical absorption and photoluminescence were investigated. The x-ray diffraction study showed that the films are well crystallized with temperatures. Strong reflection from plane (400) indicated the film's preferred growth orientation. The V2O5 films are found to be highly transparent across the visible spectrum and the measured photoluminescence quenching suggested the film's potential application in OPV device fabrication. [ABSTRACT FROM AUTHOR]

A nickel(II) Schiff base complex immobilized on multi-wall carbon nanotubes (MWCNTs) surface as a highly efficient heterogeneous catalyst was synthesized and characterized by IR, X-ray diffraction patterns, scanning electron microscopy, energy-dispersive X-ray spectroscopy, inductively coupled plasma, elemental analysis, and thermal gravimetric analysis. Then a facile and environmentally benign procedure was developed for synthesis of polyhydroquinoline derivatives via Hantzsch one-pot condensation reaction of aromatic aldehydes, 1,3-diones, ethyl acetoacetate, and ammonium acetate in the presence of above synthesized catalyst under solvent-free conditions. This protocol has the advantages of stability, easy availability, recyclability and eco-friendly nature of catalyst, simple experimental and work-up procedure, and also high to excellent yields. Considering the solvent-free condition and also temperature, time, and yield of the model reaction, the nanocatalyst reported here is among the best catalysts reported so far for synthesis of polyhydroquinolines. [ABSTRACT FROM AUTHOR]