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Buildings’ carbon emissions consist of embodied carbon (EC) associated with the production and transportation of materials and operational carbon (OC) generated from consumed energy during daily use. However, previous studies concentrated on either EC or OC but ignored an integrated analysis of their relationships. Therefore, this paper aims to explore appropriate building envelope design solutions by examining the trade-off between EC and OC of high-rise residential buildings. To achieve this aim, the life cycle assessment method was used to evaluate the EC and OC of the residential buildings using SimaPro and DesignBuilder software. A building information modelling (BIM) model was developed to extract the geometric data and material consumption. A typical 30-story public residential building in Hong Kong was examined. The EC and OC of the case building were calculated as 561 kg CO2e/m2 and 50.18 kg CO2e/m2/yr, respectively. Different low carbon design scenarios were identified from the literature review and the semi-structured interviews with designers and contractors. Results indicate that low carbon concrete is an effective approach for not only reducing the EC by 5%-15%, but saving a mild percentage of OC, and is therefore encouraged. However, changing the thickness of external walls leads to a very limited life cycle carbon reduction (0.99%). Lower U-value of envelopes is recommended as 2.84% and 2.89% life cycle carbon is reduced when adopting insulations for external walls and triple-glazing windows. The findings are valuable for examining the relationships between EC and OC and can support low carbon building decision-making.

Buildings contribute significantly to carbon emissions over their life cycle. Recently, embodied carbon (EC) accounts for an increasing share of life cycle carbon emissions of new buildings as their energy efficiency is improving. However, traditional methods of data collection and communication from diverse carbon sources such as manual recording lead to low-efficient and prone-to-error estimation of embodied carbon emissions. Therefore, this paper aims to propose an Internet of Things (IoT)-integrated embodied carbon assessment and monitoring system (ECAMS) for prefabricated buildings. This system involves three layers, i.e., of data collection, data communication, and data analysis. To provide a theoretical foundation, the EC assessment model is firstly modified to distribute carbon emissions into twelve construction statuses and at five levels of analysis of prefabricated buildings. IoT sensors including radio frequency identification (RFID), acceleration transducer, and global positioning system (GPS) are employed for automated real-time data collection. IoT data will be communicated with building information modelling (BIM) and carbon assessment software through application programming interfaces (APIs). Laboratory tests were conducted to demonstrate the feasibility of sensor-based data collection and communication. The proposed system facilitates more efficient and accurate estimations of prefabricated buildings’ embodied carbon, which should help practitioners to explore effective carbon reduction strategies.

The arylalkylamine N-acetyltransferase (AANAT) enzymes catalyze the acetyl-CoA-dependent acetylation of an amine or arylalkylamine, which is involved in important biological processes of insects. Here, we carried out the molecular and biochemical identification of an arylalkylamine N-acetyltransferase (AANAT) from the oriental fruit fly, Bactrocera dorsalis. Using a bacterial expression system, we expressed and purified the encoded recombinant BdorAANAT1-V3 protein. The purified recombinant protein acts on a wide range of substrates, including dopamine, tyramine, octopamine, serotonin, methoxytryptamine, and tryptamine, and shows similar substrate affinity (i.e., K

LuxR, a bacterial quorum sensing-related transcription factor that responds to the signaling molecule 3-oxo-hexanoyl-homoserine lactone (3OC6-HSL). In this study, we employed molecular dynamics simulation and the Molecular Mechanics Generalized Born Surface Area (MM-GB/SA) method to rationally identify residues in Vibrio fischeri LuxR that are important for its interaction with 3OC6-HSL. Isoleucine-46 was selected for engineering as the key residue for interaction with 3OC6-HSL-LuxR-I46F would have the strongest binding energy to 3OC6-HSL and LuxR-I46R the weakest binding energy. Stable wild-type (WT) LuxR, I46F and I46R variants were produced in Escherichia coli (E. coli) in the absence of 3OC6-HSL by fusion with maltose-binding protein (MBP). Dissociation constants for 3OC6-HSL from MBP-fusions of WT-, I46F- and I46R-LuxR determined by surface plasmon resonance confirmed the binding affinity. We designed and constructed a novel whole-cell biosensor on the basis of LuxR-I46F in E. coli host cells with a reporting module that expressed green fluorescent protein. The biosensor had high sensitivity in response to the signaling molecule 3OC6-HSL produced by the target bacterial pathogen Yersinia pestis. Our work demonstrates a practical, generalizable framework for the rational design and adjustment of LuxR-family proteins for use in bioengineering and bioelectronics applications.

Background Breast cancer is a common malignant tumor, threatening the general health of women worldwide. Estrogen-related receptor alpha (ERRα) is a member of nuclear receptor family and has been shown to involve in the pathophysiology of breast cancer. However, the specific relationship between ERRα and the triple negative breast cancer (TNBC), is not clear yet. Objective This study examined the relevance between ERRα expression and different clinical features of breast cancer patients. Methods The expression level of ERRα in 150 human breast cancer tissues (which contains 48 human triple negative breast cancer tissues) and 53 human benign breast tumor tissues using immunohistochemical staining. Results ERRα protein level was significantly higher in breast cancer tissues than that in benign tumors. High expression of ERRα was significantly associated with the high grade but not the clinical stage and human epidermal growth factor receptor 2 of the breast cancer tissues. Its high expression was also positively correlated with triple negative breast cancer in pathological grade 2 and 3, but not in grade 1. high expression of ERRα was positively correlated with triple negative breast cancer in each clinical stage. In addition, high expression of ERRα was associated with shorter overall survival of breast cancer patients. Conclusion In conclusion, highly expressed ERRα was associated with higher pathological grades triple-negative breast cancer and shorter overall survival. Future studies were required to recruit more patients to consolidate the current findings.

It is known that methanogens play a critical role in the carbon cycle in soil, while methanogen community characteristics and their environmental influencing factors in the soil planted with salt-tolerant rice remain unclear. In this study, methanogen abundance, community composition, and relationships with environmental factors in soils planted with the salt-tolerant rice (YC1703) and ordinary rice (Lindao 10) were evaluated in the rice improvement demonstration base of Qingdao Wisdom Agricultural Industry using real-time fluorescence quantitative PCR and Illumina high-throughput sequencing. The results indicated that the abundance and community richness of methanogens in Lindao 10 soil were significantly higher than those in YC1703 soil, and methanogens in YC1703 soil exhibited higher diversity. The combined effects of rice varieties, rice growth period, and environmental factors had impacts on the methanogen community. The hydrogenotrophic methanogens were dominant in the YC1703 and Lindao 10 soils; thus, we speculated that the dominant pathway of methane production in these soils was hydrogenotrophic methanogenesis.

The application of biodegradable chelating agents to enhance phytoremediation is a low-cost and promising method to improve the remediation efficiency of heavy metal-contaminated soil. The effects of N, N-bis glutamic acid (GLDA) on the growth and heavy metal absorption of Solanum nigrum were studied by pot experiment. The addition of chelate on the 20th day after sowing can improve the bioavailability of cadmium (Cd) in the soil. The results showed that the addition of chelating agents effectively improved the migration rate of the target heavy metal Cd in the soil, and significantly increased the accumulation of heavy metal in the roots, stems, and leaves of plants. The results showed that compared with the control group, the chelating agent could increase the extraction rate of total Cd by 28.65-68.74%. The application of GLDA significantly increased the accumulation of Cd (20 mg kg

BACKGROUND: This study aimed to explore the added prognostic value of baseline metabolic volumetric parameters and cell of origin subtypes to the National Comprehensive Cancer Network International Prognostic Index (NCCN-IPI) in nodal diffuse large B-cell lymphoma (DLBCL) patients. METHODS: A total of 184 consecutive de novo nodal DLBCL patients who underwent baseline positron emission tomography/computed tomography (PET/CT) were included in this study. Kaplan-Meier estimates were generated to evaluate the clinical, biological, and PET/CT parameters’ prognostic value. The Cox proportional hazards model was performed to examine the potential independent predictors for progression-free survival (PFS) and overall survival (OS). RESULTS: With a median follow-up of 35 months, the 3-year PFS and OS were 65.2% and 73.0%, respectively. In univariate analysis, total lesion glycolysis (TLG), cell-of-origin subtypes, and NCCN-IPI were both PFS and OS predictors. High TLG (≥1,852), non-germinal center B (non-GCB), as well as high NCCN-IPI (≥4), were shown to be independently significantly associated with inferior PFS and OS after multivariate analysis. Based on the number of risk factors (high TLG, non-GCB, and high NCCN-IPI), a revised risk model was designed, and the participants were divided into four risk groups with very different outcomes, in which the PFS rates were 89.7%, 66.2%, 51.7%, and 26.7% (χ(2)=30.179, P

Systemic lupus erythematosus (SLE) increases the risk of adverse pregnancy outcomes and fetal complications. Placenta percreta, involving placental attachment to another organ, is a rare but severe placental abnormality. We report a 26-year-old woman, G2P1, with a 6-year history of SLE with coexisting pernicious placenta previa and placenta percreta detected by second trimester ultrasound. She discontinued prednisone 5 months before admission, without consultation, and active SLE was diagnosed on admission. Because of her progressive condition, the patient underwent infrarenal abdominal aorta balloon occlusion and double J ureteral catheter placement, followed by elective cesarean at 27+6weeks gestation. Despite aggressive management, she experienced severe bleeding requiring internal iliac artery ligation and peripartum hysterectomy. The placenta had penetrated the uterus walls and attached to the bladder apex, necessitating bladder repair. Thrombosis was detected in the common iliac artery and common femoral artery in the right leg 1 day postoperatively. Conservative antithrombotic therapy had little effect, and embolectomy by arteriotomy was performed on the 6th post-cesarean day, and an arterial thrombus was removed. Infrarenal abdominal aorta balloon occlusion may increase the risk of postoperative thrombosis in pregnant women with active SLE and coagulation disorders. These patients therefore require close monitoring and timely anticoagulation.

Triclosan (TCS) is widely used in personal care products and acts as an antibacterial agent. Residues of TCS may have potential effects on the human health. In this article, the interaction between TCS and bovine serum albumin (BSA) has been characterized by using multi-spectroscopic approaches and molecular docking method under physiological conditions. Thermodynamic investigations revealed that TCS spontaneously bound to a binding site of BSA and hydrogen bonds played a dominant role in this process. The site marker competition experiments indicated that TCS bound at site II (subdomain IIIA) of BSA, which was well substantiated by molecular docking. The binding of TCS further led to changes of conformation and microenvironment of BSA. This work explored the interaction of TCS with BSA, which might be beneficial for evaluating the binding mechanism of other environmental pollutant at molecular level.

In this paper, a generalized method, fractional-order approximate entropy (FOApEn), is proposed with the objective of distinguishing the complexity of time processes from statistical perspectives and characterizing differences and changes in dynamical systems. Moreover, we generalized approximate entropy (ApEn) to multiscales, which can detect complexity of time series in more scales and probe the multiscale properties containing in time series. This fractional-order approximate entropy, which provides an assessment on the multiscale complexity between measurements, is defined in terms of the FOApEn method and the multiscale method. The implementation of multiscale FOApEn is illustrated with simulated time series and financial time series. Examples taken from simulated and financial data demonstrate that tuning the fractional order allows a high sensitivity to the signal evolution and how the FOApEn for complex systems behaves on different scales and determination, which is helpful in describing the dynamics of complex systems.

In this work, two types of transition layers (pure Cr and gradient CrNx) were fabricated between plasma-assisted nitrided (PAN) stainless steel and magnetron sputtering CrN coating to investigate the effect of transition layer on mechanical properties of CrN coating on PAN stainless steel substrates. For comparison, samples without transition layer were also synthesized. The phase structure, morphology, abrasive resistance and adhesion of the CrN coatings, as well as the nano-hardness and Young's modulus were investigated by XRD, SEM, ball-on-disk tribometer, scratch tester and nano-indentation tester, respectively. The results showed the transition layers of pure Cr and gradient CrNx absorbed the excess nitrogen diffusing outwards from the nitrided layer, and thus suppressed partly the precipitation. As a result, the adhesion force was improved to 22.5 and 25.7 N, respectively. The nano-hardness and Young's modulus of modified layers were improved to the range of 22.8–25.8 GPa and 331–347.3 GPa, corresponding to the substrate of 7.2 GPa and 230.5 GPa. The modified layer with no transition layer had the best wear resistance for nitriding of 1 h; while the layer with gradient CrNx had the best wear resistance for nitriding of 4 h.

In this paper, we propose an augmented Lagrangian proximal alternating (ALPA) method for solving two classes of large-scale sparse discrete constrained optimization problems. Specifically, the ALPA method generates a sequence of augmented Lagrangian (AL) subproblems in the out iterations and utilizes a proximal alternating linearized minimization method and sparse projection techniques to solve these AL subproblems. And we study the first-order optimality conditions for these two classes of problems. Under some suitable assumptions, we show that any accumulation point of the sequence generated by the ALPA method satisfies the necessary first-order optimality conditions of these problems or is a local minimizer of these problems. The computational results with practical problems demonstrate that our method can find the suboptimal solutions of the problems efficiently and is competitive with some other local solution methods.

This paper examines educational inequality in nine Latin American countries at the sub-country level from the 1950s to the 1990s. Educational inequality is measured by the difference in schooling years between the taller and the shorter half of the female population. Schooling years significantly increased across birth cohorts, especially before the 1980s, regardless of socio-economic stratum, region or country. However, educational inequality persisted. This finding reflects the achievement of the import substitution industrialisation era in educational development and its failure in mitigating the unequal distribution of education rooted in Latin America's social structure. Trade liberalisation and educational expansion are found to reduce educational inequality in capital and urban regions, whereas democracy and tax reform increased it. By contrast, educational inequality in rural regions was hardly influenced by policy changes. This finding urges future explorations into whether the persistence of educational inequality in rural regions is due to endemic social structure.

Gefitinib is the first-generation EGFR tyrosine kinase inhibitor (EGFR-TKI), which is used in the treatment of NCSLC patients through interrupting EGFR signaling pathway. Although gefitinib prolongs patients' progression-free survival (PFS), acquired resistance occurs in advanced NSCLC patients. In this study, we mainly investigated the effects of antagonist for ghrelin-R (D-lys-3-GHRP-6) on conquering acquired gefitinib resistance in human lung cancer cells. We found that GHSR was overexpressed in our established HCC827/GR cells compared with parental cells, accompanied with increase of p-AKT and p-ERK1/2. Treatment of D-lys-3-GHRP-6 significantly decreased p-AKT and p-ERK1/2 expression in HCC827/GR cells. H1650 cells and HCC827/GR cells were treated with control, gefitinib, D-lys-3-GHRP-6 and D-lys-3-GHRP-6 + gefitinib, respectively. In H1650 and HCC827/GR cells, combination of D-lys-3-GHRP-6 and gefitinib significantly inhibited cell proliferation and Bcl2 protein level, induced the cell apoptosis and cleaved-caspase3 protein level compared with control group, while there was no significant difference between control and gefitinib group.

Mono-cardboard waste digestion in batch tests associated with different impact factors was investigated. The maximum methane generation was 394 mL/gVSadd with the best F/M of 0.5 at mesophilic cond...

Buildings contribute to significant carbon dioxide emissions over their life cycle. The prefabrication approach has been promoted and increasingly integrated into building engineering and construction, which has attracted increasing research into the life-cycle carbon assessment (LCCA) of prefabricated buildings. However, there exist challenges. This paper examines the challenges and, through rethinking their theoretical fundamentals, proposes solutions with the aim to enhance the reliability and validity of research into the LCCA of prefabricated buildings. The challenges are found to be implicit system boundaries of prefabrication and LCCA: inconsistent methods, models and units of analysis of estimating buildings’ life-cycle carbon dioxide emissions and LCCA being a labour-intensive process with limited accountability. The proposed solutions are a regression model of relating buildings’ LCCA to 12 system boundaries, expressed by the system boundaries’ inconsistency ratio (I R), which shows the level of difference between any two system boundaries; a five-level framework of units of analysis consisting of material, component, assembly, flat and building; and a smart-technology-integrated approach for automatic LCCA. The solutions are important and timely to help reduce buildings’ life-cycle carbon dioxide emissions effectively for achieving long-term sustainability of society.

Necroptosis refers to a programmed form of necrosis, which involves the receptor-interacting protein kinase 3 (RIPK3) and mixed lineage kinase domain-like protein (MLKL). In this study, to investigate the role of necroptosis in the pathogenesis of acute-on-chronic hepatitis B liver failure (ACHBLF), we retrospectively analyzed 122 patients with ACHBLF, 131 patients with chronic hepatitis B (CHB), and 35 healthy controls (HCs). Using quantitative real-time polymerase chain reaction (RT-qPCR), we measured RIPK3 mRNA levels in peripheral blood mononuclear cells (PBMCs). ELISA was performed to measure the serum levels of MLKL, TNF-α and caspase-8. We found that RIPK3 mRNA levels were significantly higher in patients with ACHBLF than those with CHB or HCs. RIPK3 mRNA levels in patients with ACHBLF were positively correlated with serum levels of TNF-α or MLKL and negatively correlated with caspase-8 levels. Univariate and multivariate analysis revealed that RIPK3 mRNA level was predictive of 3-month mortality of ACHBLF. The area under receiver operating characteristic curve (AUC) of RIPK3 mRNA levels was 0.810 (95% CI: 0.729-0.876), which was higher than that of MELD scores (0.766, 95% CI: 0.681-0.838). The optimal cut-off point of 8.81 was determined for RIPK3 mRNA levels, which showed a sensitivity of 80.7% and a negative predictive value of 80.4%. These results indicate that elevated RIPK3 mRNA levels in PBMCs are associated with poor prognosis of ACHBLF. We thus propose that necroptosis may play an important role in pathogenesis of ACHBLF.

In this work, a facile grinding method for preparation of supramolecular square nanoplates formed by β-cyclodextrin and gallium acetylacetonate was developed. The nanoplates exhibited a highly monodispersed distribution nature at room temperature, and were self-assembled to form supramolecular layer nanosheets at 353 K. Heating to higher temperatures such as 673 K led to the decomposition of the layer nanosheets into γ-Ga2O3 layer nanosheets. The γ-Ga2O3 nanosheets (thickness of about 15 nm) with a very high specific surface area (93.9 m2 g−1) show an improved solar-blind detection performance (e.g., light–dark ratio, 1.64 × 104) compared to devices based on 3D structured γ-Ga2O3. We believe that the grinding-heating-strategy provides a convenient and efficient route to synthesize high-quality supramolecular nanocrystals and inorganic nanomaterials.

In this work, a simple one-step hydrothermal method was developed to synthesize high-quality α-Fe2O3 nanoparticles with a snowflake-like microstructure. First, a series of binary supramolecular aggregates were prepared by a non-covalent combination between a polymer such as polyvinylpyrrolidone (PVP) and a complex such as potassium ferrocyanide (PF). Then, the aggregates were used as the precursors of the one-step hydrothermal reactions. The snowflake-like nanostructure has six-fold symmetry as a whole, and each petal is symmetric. This synthesis method has the characteristics of simplicity, rapidity, reliance, and high yield, and can be used for creating high-quality α-Fe2O3 nanoparticles. Moreover, our results show that the molar ratio of PVP to PF, reaction time and temperature play important roles in the generation of a complete snowflake structure from different angles. Also, the snowflake-like α-Fe2O3 nanostructure exhibits a much higher coercivity (2997 Oe) compared to those reported by others, suggesting a strong hysteresis behaviour, which promises potential applications in memory devices, and other fields. Further, the α-Fe2O3 nanosnowflakes show a much higher photocatalytic degradation activity for cationic organic dyes such as crystal violet, rhodamine 6G than for anionic dyes such as methyl orange. A possible photocatalytic mechanism was proposed for explaining the selectivity of the photocatalytic oxidation reaction of organic dyes. We believe that this study provides a direct link among coordination compounds of transition metals, their supramolecular aggregates with polymers, and controlled hydrothermal synthesis of high-quality inorganic metal oxide nanomaterials.

To efficiently produce potato (Solanum tuberosum L.) microtubers, in vitro plantlets were pretreated with an acid solution before culturing in Murashige and Skoog medium supplemented with 60 g L−1 sucrose. The first tubers were formed at 36 h of acid pretreatment (AT), which was 6.2 d earlier than the untreated control. The maximum number of tubers (3.2), and highest fresh weight per tuber (282.5 mg) were also achieved with the AT. During microtuberization, the leaf dry weight of the control was higher than the AT from 1 to 5 d of culture. However, the dry weight of AT leaves increased rapidly after 5 d of culture and reached the same level as the control. The tuber dry weight in the AT was significantly higher (α = 0.05) than the control during the same culture period. Sucrose and starch content, and the enzyme activity of sucrose synthase (SS), ADP-glucose pyrophosphorylase (AGPase), and soluble starch synthase (SSS) in the AT and control exhibited a similar pattern as culture time progressed. However, levels of these biochemical indexes statistically differed (α = 0.05) between the AT and control. In addition to the sucrose content in AT, the starch content and activities of SS, AGPase, and SSS in the leaves were lower, whereas those in tubers were higher than those in the control. The present study suggests that acid pretreatment can efficiently promote microtuber formation and growth, which can be used for industrial production of potato seeds.

A lanthanide silylamide-catalyzed tandem reaction of isatins, diethyl phosphite, and 2,3-diarylcyclopropenones has been developed. A series of pyrano[2,3

As the demand for high-performance computing continues to grow, traditional computing models are facing unprecedented challenges. Among the many emerging computing technologies, DNA computing has attracted much attention due to its low energy consumption and parallelism. The DNA circuit, which is the basis for DNA computing, is an important technology for the regulation and processing of the molecular information. This review highlights the basic principles of DNA computing, summarizes the latest research progress, and concludes with a discussion of the challenges of DNA computing. Such integrated molecular computing systems are expected to be widely used in the fields of aerospace, information security and defense system.随着高性能计算需求的不断增长，传统计算模式面临着前所未有的巨大挑战。在众多新兴计算技术中，DNA计算系统以其低能耗、并行化等特点而广受关注。DNA电路 (DNA circuit) 是实现DNA计算的基础，也是该领域重要的分子信息调控和处理技术。文中重点介绍了DNA计算的基本原理，并总结了最新的研究进展，最后讨论了基于DNA计算所面临的挑战。此类集成的分子计算系统有望广泛应用于航空航天、信息安全及国防建设等领域。.

Coronavirus disease (COVID-19) patients frequently develop liver biochemical abnormality. However, liver biochemical abnormality in COVID-19 patients with liver cirrhosis is under-recognized.Patients hospitalized during COVID-19 pandemic in China (ie, from February to April 2020) were screened. All of 17 COVID-19 patients with liver cirrhosis consecutively admitted to the Wuhan Huoshenshan Hospital were identified. Meanwhile, 17 age-, sex-, and severity-matched COVID-19 patients without liver cirrhosis admitted to this hospital were selected as a control group; all of 14 cirrhotic patients without COVID-19 consecutively admitted to the Department of Gastroenterology of the General Hospital of Northern Theater Command were selected as another control group. Incidence of liver biochemical abnormality and decompensated events were primarily compared.Among the COVID-19 patients with liver cirrhosis, the incidence of liver biochemical abnormality at admission and during hospitalization were 76.50% and 84.60%, respectively; 7 (41.20%) had decompensated events at admission; 1 was transferred to intensive care unit due to gastrointestinal bleeding. Among the COVID-19 patients without liver cirrhosis, the incidence of liver biochemical abnormality at admission and during hospitalization were 58.80% (P = .271) and 60.00% (P = .150), respectively. Among the cirrhotic patients without COVID-19, the incidence of liver biochemical abnormality at admission and during hospitalization were 69.20% (P = .657) and 81.80% (P = .855), respectively; 11 (78.60%) had decompensated events at admission (P = .036). None died during hospitalization among the three groups.Liver biochemical abnormality is common in COVID-19 patients with liver cirrhosis. Management of decompensated events in cirrhotic patients without COVID-19 should not be neglected during COVID-19 pandemic.

Background This study sought to examine the efficacy and adverse reactions of capecitabine and lobaplatin in the treatment of metastatic human epidermal growth factor receptor 2 (HER-2) negative breast cancer (BC). Methods This retrospective study examined 45 patients diagnosed with advanced HER-2 negative BC. Patients were enrolled in this study from November 2015 to June 2019. The patients received capecitabine and lobaplatin combination therapy. The therapeutic efficacy and side effects were evaluated after at least 2 cycles of treatment. Results Therapeutic efficacy and adverse reactions were evaluated in 38 patients, comprising 12 cases of partial response (PR), 19 cases of stable disease (SD), and 7 cases of progressive disease (PD). Among these, 3 patients required treatment delays or dose reductions for subsequent cycles, and 2 patients discontinued treatment. The overall response rate (ORR) was 31.58% and the disease control rate (DCR) was 81.58%. The ORR and DCR for hormone receptor positive, HER-2 negative (HR+/HER-2-) and triple negative breast cancer (TNBC) patients were 31.82% and 31.25%, and 86.36% and 75%, respectively. The median progression free survival (PFS) was 8 months, 6 months, and 6 months in patients receiving the therapeutics as a first-line, second-line, or third-line and beyond treatment, respectively. The main side effects were myelosuppression, including granulocytopenia, thrombocytopenia, and anemia. Among patients with grade 1 side effects or above, 28 patients (73.68%) had myelosuppression, and 13 patients (34.21%) had gastrointestinal reactions. Further, we investigated the association between side effects and clinical outcomes, and found that PFS was increased in patients with myelosuppression and gastrointestinal reactions. Conclusions Capecitabine and lobaplatin combination therapy was effective and well tolerated among patients with advanced HER-2 negative BC.

As an example of Shenyi capsule on non-small-cell lung cancer combined with chemotherapy, this review discusses the synergistic effect and mechanism of natural drugs in oncotherapy from the yin-yang perspective in ancient Chinese philosophy, so as to reflect the therapeutic principle of natural drugs for tumor more comprehensively. The major focuses of this review are on the philosophical thinking of yin-yang as a tool which can not only explain the effect of Shenyi capsule in NSCLC combined with chemotherapy but also explore the mechanism of Shenyi capsule at the cellular and molecular level. Learning from the “yin-yang” thinking of ancient Chinese philosophy will bring more enlightenment to the research and development of traditional Chinese drugs in the future.

BACKGROUND Most of the widely used methods for the assessment of keloid treatment are subjective grading scales based on the opinion of an individual clinician or patient. There is a growing need for objective methods to evaluate keloid treatment. OBJECTIVE This study aimed to evaluate the value of laser speckle contrast imaging (LSCI) as an objective method for the assessment of dual-wavelength laser therapy for keloids. METHODS This prospective study included 21 patients with 54 keloids. All patients were treated with a combined 585-nm pulsed dye laser and 1,064 nm neodymium-doped yttrium aluminum garnet dual-wavelength laser at 4 weeks to 6 weeks intervals. Keloids were assessed using the Vancouver Scar Scale (VSS) and LSCI. RESULTS The total VSS score significantly decreased after 4 sessions of treatment (p < .05). Blood perfusion in keloids as measured by LSCI was significantly reduced after treatment (p < .05). The improvement of chest keloids in terms of the total VSS score and blood perfusion was significantly greater than that of scapular keloids (p < .05). There was a positive correlation between decreased perfusion and reduced total VSS score (R2 = 0.84). CONCLUSION Blood perfusion in keloids significantly decreased after dual-wavelength laser therapy. Laser speckle contrast imaging is a promising objective method for assessing the improvement of keloids treated with laser therapy.

Adipocytes play pivotal roles in maintaining energy homeostasis by storing lipids in adipose tissue (AT), regulating the flux of lipids between AT and the circulation in response to the body's energy requirements and secreting a variety of hormones, cytokines and other factors. Proper AT development and function ensure overall metabolic health. Nuclear factor erythroid 2-related factor 1 (NFE2L1, also known as NRF1) belongs to the CNC-bZIP family and plays critical roles in regulating a wide range of essential cellular functions and varies stress responses in many cells and tissues. Human and rodent Nfe2l1 genes can be transcribed into multiple splice variants resulting in various protein isoforms, which may be further modified by a variety of post-translational mechanisms. While the long isoforms of NFE2L1 have been established as master regulators of cellular adaptive antioxidant response and proteasome homeostasis, the exact tissue distribution and physiological function of NFE2L1 isoforms, the short isoforms in particular, are still under intense investigation. With regard to key roles of NFE2L1 in adipocytes, emerging data indicates that deficiency of Nfe2l1 results in aberrant adipogenesis and impaired AT functioning. Intriguingly, a single nucleotide polymorphism (SNP) of the human NFE2L1 gene is associated with obesity. In this review, we summarize the most significant findings regarding the specific roles of the multiple isoforms of NFE2L1 in AT formation and function. We highlight that NFE2L1 plays a fundamental regulatory role in the expression of multiple genes that are crucial to AT metabolism and function and thus could be an important target to improve disease states involving aberrant adipose plasticity and lipid homeostasis.

Introduction & objectives The independent effect of liver biochemistries as a prognostic factor in patients with COVID-19 has not been completely addressed. We aimed to evaluate the prognostic value of abnormal liver tests on admission of hospitalized patients with COVID-19. Materials & methods We performed a prospective cohort study including 1611 hospitalized patients with confirmed SARS-CoV-2 infection from April 15, 2020 through July 31, 2020 in 38 different Hospitals from 11 Latin American countries. We registered clinical and laboratory parameters, including liver function tests, on admission and during hospitalization. All patients were followed until discharge or death. We fit multivariable logistic regression models, further post-estimation effect through margins and inverse probability weighting. Results Overall, 57.8% of the patients were male with a mean age of 52.3 years, 8.5% had chronic liver disease and 3.4% had cirrhosis. Abnormal liver tests on admission were present on 45.2% (CI 42.7–47.7) of the cohort (n = 726). Overall, 15.1% (CI 13.4–16.9) of patients died (n = 244). Patients with abnormal liver tests on admission presented higher mortality 18.7% (CI 15.9–21.7), compared to those with normal liver biochemistries 12.2% (CI 10.1–14.6); P 30. Conclusions The presence of abnormal liver tests on admission is independently associated with mortality and severe COVID-19 in hospitalized patients with COVID-19 infection and may be used as surrogate marker of inflammation. Clinicaltrials.gov NCT04358380.

Zhou X, Feng Y, Liu S, et al. Cancer Manag Res.2021;13:2379–2388. The authors have advised that the funding statement on page2387 is incorrect. The text “The project was supported byCadre Health Research Project of Jiangsu Province(BJI18033) and Youth Project of Nanjing MedicalUniversity Affiliated Cancer Hospital (ZN201603)” shouldread “The project was supported by Cadre Health ResearchProject of Jiangsu Province (BJ18033) and Youth Project ofNanjing Medical University Affiliated Cancer Hospital(ZN201603)”. The authors apologize for this error. Read the original article

Hyperaccumulators store metals in the vacuoles of leaf cells. To investigate the role of vacuolar compartmentalization in Cd accumulation, chelation and induced antioxidation, we quantified the amounts of total cadmium (Cd), Cd2+, glutathione (GSH) and reactive oxygen species (ROS) in leaf cells of Solanum nigrum L. The results confirmed that vacuoles were, indeed, the main storage compartments for Cd. We then found that with increased Cd treatment concentration, the proportion of vacuolar Cd in protoplasts showed its ultimate storage capacity (82.24 %-83.40 %), and the Cd concentration stored in the protoplast maintained at a certain level (73.81-77.46 mg L-1). Besides, studies on different forms of Cd showed that the chelation state was dominant in the protoplast. The large level appearance of Cd2+ outside the vacuole revealed the limitations of vacuolar Cd2+ sequestration. The relationships between the combined forms of Cd and GSH outside the vacuole (R2 = 0.9906) showed GSH was mainly distributed to important compartments for chelation, not to vacuoles. We also demonstrated the presence of ROS-induced oxidative stress and detoxification mediated by the antioxidant GSH in vacuoles, suggesting that sequestration into vacuoles is an active process accompanied by chelation and antioxidant-mediated detoxification.

Purpose: The purpose of this study was to investigate the prognostic relevance of metabolic parameters measured using 18F-FDG PET/CT in patients with metastatic cutaneous malignant melanoma (CMM).Materials and Methods: The prognostic impact of whole-body metabolic tumor volume (wMTV), whole-body tumor lesion glycolysis (wTLG), maximum standardized uptake value (SUVmax) and mean standardized uptake value (SUVmean) was evaluated in 42 metastatic CMM patients who underwent 18F-FDG PET/CT. The metabolic parameters were dichotomized by optimal cutoff values using time-dependent receiver operating characteristic (ROC) curves. In addition, univariate and multivariate analyses of the metabolic parameters were performed using the Kaplan-Meier method and Cox proportional hazards models.Results: The optimal cutoff values for disease-free survival (DFS) were 4.63 for SUVmax, 3.31 for SUVmean, 8.22 cm3 for wMTV, and 18.22 for wTLG. The optimal cutoff values for melanoma-specific survival (MSS) were 4.77 for SUVmax, 3.31 for SUVmean, 22.32 cm3 for wMTV, and 51.37 for wTLG. Thirty-two (72%) of the 42 patients experienced recurrence during the follow-up period, and 21 patients (50%) died from the disease. In univariate analysis, SUVmax greater than 4.63 (p= 0.025) and SUVmean greater than 3.31 (p= 0.011) affected DFS, while SUVmax greater than 4.77 (p= 0.039), wMTV greater than 22.32 (p= 0.023) and wMTV greater than 51.37 (p= 0.016) affected MSS. In multivariate analysis after adjustment for the effects of clinical parameters, SUVmax was the best predictive factor for DFS (p = 0.016), and SUVmax, wMTV and wTLG were the best predictive factors for MSS (p = 0.023, p = 0.018, and p = 0.007).Conclusions: SUVmax appears to be a strong independent prognostic factor for recurrence in metastatic CMM, and SUVmax, wMTV and wTLG were found to be the best predictive markers for melanoma-specific death.

As an example of Shenyi capsule on non-small-cell lung cancer combined with chemotherapy, this review discusses the synergistic effect and mechanism of natural drugs in oncotherapy from the yin-yang perspective in ancient Chinese philosophy, so as to reflect the therapeutic principle of natural drugs for tumor more comprehensively. The major focuses of this review are on the philosophical thinking of yin-yang as a tool which can not only explain the effect of Shenyi capsule in NSCLC combined with chemotherapy but also explore the mechanism of Shenyi capsule at the cellular and molecular level. Learning from the “yin-yang” thinking of ancient Chinese philosophy will bring more enlightenment to the research and development of traditional Chinese drugs in the future.

The high moisture content of lignite restricts its extensive and efficient use. Furthermore, the reabsorption of lignite is also a factor that affects lignite spontaneous combustion. Therefore, it is of great importance to study the process and mechanism of water molecule desorption and adsorption on lignite and coke (25–950°C) to achieve the clean and efficient utilization of lignite and environmental protection. Proton nuclear magnetic resonance (1H-NMR), thermogravimetric analysis, and other techniques were used in this study to explore the water molecule absorption and desorption processes of lignite pyrolysis at different temperatures (25–950°C) and the special contributions of ether bonds to water molecule adsorption. A mechanism of lignite water molecule adsorption was proposed. The results showed that ether bonds played a special role in the water molecule adsorption by pyrolyzed lignite. The ether bond content was greater in the coal samples at 300 and 950°C, which changed the trend of lignite water molecule absorption and the distribution of water (T2) detected in the 1H-NMR experiments and delayed the escape of water molecules during moisture desorption. The total amount of adsorbed water decreased first and then increased in the coal samples as the pyrolysis temperature increased. However, the maximum adsorption interactions of each coal sample increased first and then decreased. This was the result of the interactions between the pores and the oxygen-containing functional groups. Based on the above analysis, water molecule adsorption mechanism models of lignite and coke were constructed. This study offers a new approach for investigating the water molecule adsorption and adsorption mechanisms of lignite and coke.

In this paper, one method to synthesize the large depth-of nondiffracting beam is proposed by applying the ring focus reflectarray antenna (RA) which operates at 35 GHz. The synthesis method and operation principle of this RA is specified to design the required phase distribution of the aperture. The phase of the single unit can be adjusted by controlling the patch size of the single element. This system can be utilized in many applications like near-field (NF) Wireless Power Transmission (WPT), NF detection and microwave imagine. The presented antenna owns the merits of low profile, easy to fabricate and low cost.