228 results on '"Xie JL"'
Search Results
202. [Study on the regular pattern of the distribution of skin epidermal stem cells in the different parts of a healthy human body].
- Author
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Chen XD, Li TZ, Qi SH, Xie JL, Xu YB, Pan S, Yuan JS, Zhang T, and Liang HZ
- Subjects
- Humans, Immunohistochemistry, Integrin beta1, Male, Epithelial Cells cytology, Skin cytology, Stem Cells
- Abstract
Objective: To investigate the regular pattern of the distribution of skin epidermal stem cells (ESCs) in the different parts of a healthy human body, and to evaluate the feasibility of the identification of ESCs by P63 and CD29 with single and double labeling., Methods: Full-thickness skin samples from 21 parts (including scalp, dorsum of foot, sole of foot, pubic region, and scrotum) of 5 healthy persons were harvested for the study. Immunohistochemistry method with biotin-streptavidin-horseradish peroxidase (SP) was employed with P63 and CD29 as the first antibody to carry out single and double labeling. The staining results were subjected to image analysis. The distribution of the ESCs in the skin from the above parts was observed and expressed as positive unit (PU) value., Results: It was found by P63 single labeling and P63 and CD29 double labeling that the PU value in the dorsum of foot was the lowest while that in the scalp was the highest among all the parts of a healthy body. It was also found by CD29 single labeling that the PU value in the dorsum of foot was the lowest [(11.9 +/- 1.5)%] while highest in the scalp [(29.1 +/- 5.0)%]. The PU value in the hairy region of a human body was evidently higher than that in the non-hairy region (P < 0.01), when examined by P63 and CD29 single and double labeling. But there was no difference in the PU values between the trunk and limbs by means of P63 and CD29 single and double labeling (P > 0.05)., Conclusion: There are more ESCs in the skin from the scalp, mons pubis and scrotum than other parts of the body. Single P63 or CD29 labeling exhibits higher sensitivity but lower specificity in the identification of ESCs. While the double labeling method exhibits higher specificity but lower sensitivity. Above all, it seems that the double labeling may be a simple and effective method for the identification of ESCs.
- Published
- 2006
203. [Protective effects of urinastatin on organ function in patients with severe burn].
- Author
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Xu YB, Qi SH, Xie JL, Yuan JS, Zhang T, Chen XD, Shu B, Liu P, and Li TZ
- Subjects
- Adolescent, Adult, Burns drug therapy, Female, Humans, Male, Middle Aged, Multiple Organ Failure prevention & control, Vital Signs, Young Adult, Burns physiopathology, Glycoproteins therapeutic use, Trypsin Inhibitors therapeutic use
- Abstract
Objective: To investigate the protective effects of urinastatin on organ function in severe burn., Methods: Seventy-two cases with comparative severity in burn injury were randomly divided into urinastatin treatment group (n=36) and control group (n=36). Patients in control group received routine therapy, while those in treatment group received intravenous dripping of urinastatin twice a day for 5 to 7 days. The dosage of urinastatin was 300 kU in severe burns and 200 kU in moderate burns. Both polyvinylpyrrolidone iodine (PVP-I) and Jiedu Shaoshang cream were used for wound dressing. The clinical findings were assessed and variables indicating functions of internal organs, including liver (alanine aminotransferase), kidney (blood urea nitrogen, serum creatinine), heart (aspartate aminotransferase, lactate dehydrogenase, alpha-hydroxybutyrate dehydrogenase, creatine kinase, isoenzymes of creatinine kinase), and coagulation (prothrombin time, international normalized ratio, activated partial thromboplastin time, fibrinogen), as well as blood routine test, troponin, myoglobin, arterial blood analysis, bacteria culture were measured., Results: In treatment group, vital signs and general condition were satisfactory at shock phase and peri-operative stages in all the patients. Edema of burn wound subsided rapidly. The 28-day mortality rate was 0. There was significant difference between the two groups (all P<0.05)., Conclusion: Urinastatin has protective effects on multiple organs in severe burn injury.
- Published
- 2006
204. [Influence of aerosols on the expression of cyclin B1, cyclin C and proliferating cell nuclear antigen in wound tissue healing of burned rat models].
- Author
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Zhang T, Li TZ, Xu YB, Qi SH, Liang HZ, Xie JL, Chen XD, and Zheng SS
- Subjects
- Aerosols, Animals, Burns metabolism, Cyclin B1, Cyclin C, Disease Models, Animal, Female, Rats, Rats, Sprague-Dawley, Burns therapy, Cyclin B biosynthesis, Cyclins biosynthesis, Electric Stimulation Therapy methods, Proliferating Cell Nuclear Antigen biosynthesis, Wound Healing physiology
- Abstract
Objective: To investigate the influence of aerosols on the expression of cyclin B(1), cyclin C and proliferating cell nuclear antigen (PCNA) in wound tissue healing of burned rat models., Methods: Sprague Dawley (SD) rats were inflicted as the deep partial thickness burn models. Rats were randomly divided into experimental group and control group. The experimental group were treated with aerosols. Samples were collected in 1 approximately 10 postburn days. Immunohistochemistry and image analysis methods were conducted to examine the expression of cyclin B(1), cyclin C and PCNA in both experimental and control groups., Results: The expression of cyclin C in experimental group was detected in nucleus of skin basal cell on the second postburn day, increased evidently at the fifth days and sustained at high expression level up to the tenth days after treatment. The expression of cyclin C in experimental group was significantly higher than control group (P < 0.05). The expression of PCNA was first observed in skin basal cell nucleus and hair follicle cell nucleus in both experimental and control group on the third postburn day. The expression of PCNA increased evidently at the fifth days in experimental after treatment and that increased evidently at the seventh days in control group, which showed there were lots of active proliferation cell. And the difference of the expression of PCNA between experimental and control group was significant (P < 0.01). The expression of cyclin B(1) was detected in nucleus and cytoplasm of skin basal cell in both groups on the third postburn day, and no difference between the experimental and control group (P > 0.05)., Conclusions: Aerosols can up-regulate the expression of cyclin C and PCNA in skin basal cell nucleus. Therefore the aerosols can accelerate wound tissue healing.
- Published
- 2005
205. [Clinical study and pathological examination on the treatment of deep partial thickness burn wound with negative charge aerosol].
- Author
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Li TZ, Xu YB, Hu XG, Shen R, Peng XD, Wu WJ, Luo L, Dai XM, Zou YT, Qi SH, Wu LP, Xie JL, Deng XX, Chen E, and Zhang HZ
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Disease Models, Animal, Female, Humans, Infant, Male, Middle Aged, Rats, Rats, Wistar, Young Adult, Aerosol Propellants therapeutic use, Burns pathology, Burns therapy, Wound Healing
- Abstract
Objective: To investigate the effect of negative charge aerosol (NCA) on the treatment of burn wound., Methods: Patients with superficial or deep partial thickness burn only were enrolled in the study, and they were randomly divided into trial group (T, including 180 cases of superficial thickness burn and 100 cases of deep partial thickness burn), control group (C, including 30 cases with superficial thickness burn and 30 with deep partial thickness burn), and self control group (SC, including 10 cases with superficial thickness burn and 10 with deep partial thickness burn). The patients in T and SC groups were treated with NCA for 1.5 hours, 1-2 times a day, from 6 postburn hour (PBH) to 2 postburn day (PBD), while those in C group received conventional treatment. For those in SC group, some of the wounds were covered with sterile schissel, while other wounds without schissel covering. The general changes in the wounds during NCA treatment were observed, and bacterial culture before and after NCA treatment was performed. The healing time was recorded and the blood biochemical parameters were determined. Rat model with deep partial thickness scald was established, and the rats were also divided into T and C groups, and received treatment as in human. Tissue samples were harvested from the wounds of rats in the 2 groups before and 1, 2, 3 weeks after treatment for pathological examination., Results: There was no infection and little exudation in the patients in T group. No bacteria were found in the wound before and after NCA treatment. The healing time of the wounds of patients with superficial and deep partial thickness burn in T group was 6.3 +/- 1.6 d and 15.1 +/- 3.1 d, respectively, which was obviously shorter than those in C group (11.3 +/- 1.4 d and 21.2 +/- 1.4 d, P < 0.01). In SC group, the healing time of those with sterile schissel coverage was also significantly shorter than those without covering (P < 0.01). There was no obvious change in the liver and kidney functions and blood biochemical parameters among the patients. Pathological examination showed that the skin structure was almost recovered in the rats in T group 3 weeks after treatment, while those in C group was not., Conclusion: Negative charge aerosol is safe and effective in promoting wound healing of the patients with partial thickness burns.
- Published
- 2005
206. Two new dihydroisocoumarins from the fungus Cephalosporium sp. AL031.
- Author
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Bi YM, Bi XB, Chen YT, and Xie JL
- Subjects
- Isocoumarins, Molecular Structure, Acremonium chemistry, Coumarins chemistry
- Abstract
Two new dihydroisocoumarins, 3,4-dihydro-3-heptantrienyl-8-hydroxyl-6-hydroxymethyl-5-methylisocoumarin and 6-acetoxymethylene-3,4-dihydro-3-heptantrienyl-8-hydroxyl-5-methylisocoumarin were isolated from a culture broth of a strain of the fungus, Cephalosporium sp. AL031. Their structures were elucidated by interpretation of their spectroscopic data.
- Published
- 2005
- Full Text
- View/download PDF
207. Effect of thermal treatment on carbon-doped silicon oxide low dielectric constant materials.
- Author
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Xie JL, Lin JY, Wang YH, Narayanan B, Wang MR, and Kumar R
- Subjects
- Hot Temperature, Hydrocarbons chemistry, Microscopy, Electron, Scanning, Models, Chemical, Oxygen chemistry, Oxygen metabolism, Silicon chemistry, Spectrometry, X-Ray Emission, Temperature, Time Factors, Carbon chemistry, Nanotechnology methods, Siloxanes chemistry
- Abstract
Carbon-doped silicon oxide (SiOCH) low dielectric constant (low-k) material is a good candidate for advanced interconnect technology. Good thermal stability of the dielectric is required due to the many thermal processes involved during IC fabrication. The thermal stability of tetramethylcyclotetrasiloxane (TMCTS) based plasma-enhanced chemical vapor deposition (PECVD) carbon doped low-k material with annealing temperature from 400 to 800 degrees C in N2 was studied. The thermal stability temperature of TMCTS based carbon doped low-k material is 600 degrees C. Above 600 degrees C annealing, the thermal energy can break Si-CH3, Si-C, Si-H, and C-H bonds leading to outgasing, which results in film composition change, weight loss, and thickness shrinkage. Film composition changes, especially carbon loss and oxygen incorporation, can degrade its reliability extremely. Carbon is desorbed in the form of CH4, CO, and other hydrocarbon.
- Published
- 2005
- Full Text
- View/download PDF
208. [Renal parenchyma incision and calyceal plasty in the treatment of 37 cases of complicated renal calculus].
- Author
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Xie JL, Zhou C, and Xiao JW
- Subjects
- Adolescent, Adult, Aged, Child, Female, Humans, Male, Middle Aged, Kidney surgery, Kidney Calculi surgery, Kidney Calices surgery
- Published
- 2004
209. [Factors of effects on transformation behaviors of inorganic mineral matters in desulfurizing process of burning coal].
- Author
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Xie JL, Luo JP, Zhao GJ, and Yang KB
- Subjects
- Calcium Compounds analysis, Industrial Waste prevention & control, Air Pollutants analysis, Air Pollution prevention & control, Coal analysis, Incineration, Sulfur analysis
- Abstract
The Heshan coal and the Wansheng coal were selected to investigate the effects of Ca/S molar ratio, combustion temperature and other factors on the desulfurization efficiency when limestone as a desulfurizer. And then the possible effects of various desufurizing systems on the mineral constituents of their residues were discussed by using the X-ray diffraction analyzer. Moreover, the phase diagrams of the ternary system CaO-Al2O3-SiO2 were further used in order to find out the transformation behaviors of inorganic mineral matters in the desulfurizing processes of burning coal on the basis of the XRD results. The results show that the combustion temperature and the Ca/S molar ratio are the key factors affected the desulfurization efficiency and their residues' mineral constituents. When the temperature raised and the Ca/S ratio increased, the solid-phase reactions between CaO and some inorganic matters in coal ash will be obviously accelerated, which thus are good for the formaion of many mineral matters such as C4AF,C2AS and beta-C2S. Yet the anhydrite formed will decompose if the temperature is above 1050 degrees C and then the desulfurization efficiency will decrease. The desulfurization efficiency in different temperature is mainly decided by the existence form of the sulfur-contained mineral matters and their contents in the residue. In the experiment, the best desulfurization efficiency for the HS coal was about 90% when the Ca/S ratio was 2 or 3 and the range of the temperature was from 850 degrees C to 950 degrees C.
- Published
- 2004
210. Surface-enhanced Raman scattering (SERS) from different azobenzene self-assembled monolayers and sandwiches.
- Author
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Zhang WW, Ren XM, Li H, Xie JL, Lu CS, Zou Y, Ni ZP, and Meng QJ
- Abstract
Self-assembled monolayers (SAMs) of functionalized azobenzene thiols (RAzoCnSH, n=3-6 for R=H, abbreviated as AzoCnSH; and n=4 for R=CH(3)CONH, abbreviated as aaAzoC4SH) on different substrates RAzoCnSz.sbnd;z.sfnc;S (S represents substrates of vacuum-deposited gold (Au), silver foil (Ag), HNO(3) etched silver foil (EAg), and silver mirror (mAg)) have been studied by SERS in the near-infrared region. SERS of the SAMs on EAg and/or mAg exhibit SERS effects that vary with etching time and/or deposition time. The most appropriate time is 5 s for etching in 1:1 HNO(3) and 40 s for deposition in 0.1 M Ag(NH(3))(2)NO(3). Further, a layer of Ag mirror was conveniently deposited on the top of the SAMs on different substrates, yielding a more efficient SERS-active system possessing a "sandwiched" structure of mAgz.sfnc;RAzoCnS-z.sfnc;S. An appropriate surface roughness is required for the strongest SERS effect. Scanning electron microscopy (SEM) indicates that there exist a large number of projects around 100 nm on the surface showing the strongest SERS effect. When the surface roughness is decreased or increased, the SERS effect decreases sharply. The relationship between the SERS effect and the structural nature was investigated and showed that the enhancement factor decays exponentially with increasing in distances of the azobenzene group from the underlying substrate or the overlying silver mirror. This result reveals that the SERS effect may be the result of the electromagnetic coupling effect between two metal layers.
- Published
- 2003
- Full Text
- View/download PDF
211. [Comparison of the effects of rhEGF with rhbFGF on the acceleration of wound healing].
- Author
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Xing BR, Li TZ, Bian HN, Qi SH, Xie JL, Xu YB, and Hu XG
- Subjects
- Animals, Female, Integrin beta1 genetics, Male, Microscopy, Electron, RNA, Messenger analysis, Rabbits, Recombinant Proteins pharmacology, Epidermal Growth Factor pharmacology, Fibroblast Growth Factor 2 pharmacology, Wound Healing drug effects
- Abstract
Objective: To investigate the mechanism and the accelerating effect of rhEGF and rhbFGF on wound healing., Methods: Twelve New Zealand rabbits with 72 incised wounds on ventral side of 24 ears were randomly divided into two therapeutic groups (rhEGF of 10 ug/cm(2) and rhbFGF of 100 AU/cm(2)) and a control group (1% silver sulfadiazine cream, SD-Ag). The general conditions of the wound healing was observed grossly. Biopsies were harvested at different time points for the pathomorphological examination, the electron microscopic examination, and for assessment of integrin beta1 mRNA expression by in situ hybridization., Results: The expressions of integrin beta 1 mRNA in two therapeutic groups were significantly higher than that of control group. The quality of the wound healing was improved in therapeutic group with its healing time shortened when compared with that in control group (P < 0.05). There was an obvious difference in the number of fibroblasts and capillary gemmules between the therapeutic and control groups (P < 0.05)., Conclusion: The wound healing and quality could be improved by both rhEGF and rhbFGF, but rhbFGF seemed better to be employed during the early and middle stages of the wound repair for the growth of granulation tissue, while rhEGF should be applied at the late stage of wound repair to accelerate the re-epithelialization of the wound. Combined application of rhEGF with rhbFGF according to time effect could be more beneficial to the wound repair.
- Published
- 2003
212. [A preliminary study on the identification and distribution of epidermal stem cells in different degrees of burn wounds in scalded rats].
- Author
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Xie JL, Li TZ, Qi SH, Bian HN, Cheng JD, Xu YB, and Liang HZ
- Subjects
- Animals, Burns metabolism, Female, Immunohistochemistry, Integrin alpha2beta1 analysis, Keratin-10, Keratins analysis, Male, Rats, Rats, Sprague-Dawley, Burns pathology, Stem Cells pathology
- Abstract
Objective: To investigate the distribution of epidermal stem cells (ESCs) in different degrees of burn wounds in scalded rats., Methods: Thirty-two Sprague-Dawley (SD) rats were employed in the study. First degree (I), shallow (shallow II) and deep partial thickness (deep II) and full thickness burn wounds (III) were created on the rat skin. Burn wound samples were harvested at 24 postburn hours (PBHs) from all the wounds and were processed to tissue slices. The tissue slices were stained by immunohistochemistry technique. The expression and distribution of ESCs in different degrees of burn wounds were observed with integrins alpha 2 beta 1 and keratin 10 (K10) as first antibodies., Results: K10 positive cells were found to distribute in the strata spinosum, granulosum and lucidum in the first degree burn wound (I) with large amounts of integrins alpha 2 beta 1 positive cells in the residual basal layer and skin appendages (hair follicles) in shallow partial thickness burn wound (shallow II degree), and there were less integrins alpha 2 beta 1 positive cells in the remaining skin appendages in deep dermis in deep partial thickness burn wound (deep II degree). Finally, integrins alpha 2 beta 1 positive cells were sparsely found in the III degree burn wound., Conclusion: The distribution of ESCs in burn wounds was closely related to the depth of burn wound. The residual ESCs might be the origin of burn wound regeneration and reepithelization.
- Published
- 2003
213. [Expression and characterization of Kringle 1-5 domains of human plasminogen].
- Author
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Zhou QW, Xie JL, Xin L, Ye Q, Li ZP, and Gan RB
- Subjects
- Animals, Cattle, Cell Cycle drug effects, Cell Division drug effects, Dose-Response Relationship, Drug, Endothelial Cells drug effects, Fibroblast Growth Factor 2 pharmacology, Humans, Pichia genetics, Plasminogen genetics, Plasminogen physiology, Recombinant Proteins isolation & purification, Recombinant Proteins pharmacology, Reverse Transcriptase Polymerase Chain Reaction, Kringles physiology, Plasminogen chemistry, Recombinant Proteins biosynthesis
- Abstract
The cDNA encoding Kringle 1-5 domains of human plasminogen (designated as K1-5), obtained from HepG2 by RT-PCR, was cloned into expression vector pHIL-S1. The recombinant plasmid pHIL-K1-5 was transformed into Pichia pastoris GS115 and the recombinant yeast was induced by methanol to express the recombinant protein. The expressed protein was purified by lysine affinity chromatography. The recombinant K1-5 inhibited the growth of bovine aortic endothelial cells (BAEC) stimulated by the basic fibroblast growth factor (bFGF), in a dosage-dependent manner, with a half maximal concentration of 14 mg/L. And rhK1-5 inhibited 47% of the BAEC migration stimulated by bFGF at the concentration of 50 mg/L. rhK1-5 also affected the cell cycle of BAEC and caused G(0)-G(1) arrest at the concentration of 14 mg/L.
- Published
- 2003
214. [Feeding of mixed-carbon-resource during the expression phase in cultivation of recombinant Pichia pastoris expressing angiostatin].
- Author
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Xie JL, Zhou QW, Zhang L, Ye Q, Xin L, Du P, and Gan RB
- Subjects
- Angiostatins genetics, Angiostatins physiology, Biotechnology methods, Fermentation physiology, Glycerol metabolism, Methanol metabolism, Oxygen metabolism, Pichia genetics, Angiostatins metabolism, Carbon metabolism, Pichia metabolism
- Abstract
A recombinant strain of Pichia pastoris with a phenotype of Muts was used to produce angiostatin in a 5-L fermentor. The methanol utilization ability of the present strain was weak, which resulted in extremely low growth rate and angiostatin productivity during the expression phase with methanol as the sole carbon source. To enhance the cell density and angiostatin expression level, mixed-carbon-source of glycerol-methanol was used in the expression phase. The methanol concentration was well controlled at 5 g/L by a methanol sensor and control system, and glycerol was continuously fed into the fermentor to achieve a higher cell density. 120 g/L of cells and 39 mg/L of angiostatin were reached at the end of fermentation which lasted 110 h. The mean specific cell growth rate in the expression phase was 0.01 h(-1), and the mean specific angiostatin productivity was 0.006 mg/(g x h). According to the data obtained in several runs of fermentation in which glycerol was fed at different rates, a higher mean specific angiostatin productivity was reached at the mean specific cell growth rate of 0.012 h(-1). To avoid the repression of angiostatin expression caused by residual glycerol and ethanol accumulation due to overfeeding of glycerol, glycerol addition was controlled to produce continuous oscillations in dissolved oxygen, because the change of dissolved oxygen concentration could deliver the information of available carbon source in the fermentation broth. Controlled glycerol feeding also avoided the problem of oxygen limitation brought by high cell density, and thus decreased the cooling requirement of the fermentor. Cell density reached 150 g/L at the end of fermentation, and angiostatin level reached 108 mg/L after an expression period of 96 h when the mean specific growth rate was maintained at 0.012 h(-1) by using the glycerol feeding strategy to result in the oscillations in dissolved oxygen. The mean specific angiostatin productivity was improved to 0.02 mg/(g x h). The apparent cell yield on glycerol and methanol were respectively 0.69 g/g and 0.93 g/g, higher than those in the fermentation without using the feeding strategy with dissolved oxygen as the indicator of metabolism.
- Published
- 2003
215. [A research of endothelial cell-targeted therapy for cure of hypertrophic scar].
- Author
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Shen R, Li TZ, Qi SH, Liang HZ, Xu YB, Xie JL, Wu YF, Feng XS, and Du YJ
- Subjects
- Animals, Apoptosis, Cicatrix, Hypertrophic chemically induced, Collagen Type I, Collagen Type III, Disease Models, Animal, Feasibility Studies, Neovascularization, Pathologic etiology, Vascular Endothelial Growth Factor A, Burns complications, Cicatrix, Hypertrophic therapy, Endothelial Cells
- Abstract
Objective: To investigate the feasibility of endothelial cell-targeted therapy to cure post-burn hypertrophic scar., Methods: A hypertrophic scar animal model was made. Intralesional injecting of VEGF monoclonal antibody was performed for three weeks. The changes of scar in volume and morphology were observed., Results: 1. The volume of scar decreased. 2. The number of the capillary, the amount of collagen I and collagen III decreased. 3. Transmission electron microscope examinations demonstrated many dead or apoptotic fibroblasts and endothelial cells. Fibrocytes were seen relatively common., Conclusion: VEGF induces the growth and development of hypertrophic scar in that it induces excessive and uncontrollable angiogenesis, which favors excessive collagen synthesis. Endothelial cell-targeted therapy may be a promising method to cure post-burn hypertrophic scar.
- Published
- 2003
216. An antitumor agent of beta-cyclodextrin-modified titanocene complex: synthesis and characterization.
- Author
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Lu CS, Zou Y, Xie JL, Ni ZP, Zhu HZ, Meng QJ, and Yao YG
- Subjects
- Antineoplastic Agents chemical synthesis, Cyclodextrins chemical synthesis, Growth Inhibitors chemical synthesis, Organometallic Compounds chemical synthesis, beta-Cyclodextrins
- Abstract
The complex of a derivative of beta-cyclodextrin, that is mono[6-deoxy-6-(2-butenedinitrile-2,3-dimercapto sodium salt)]-beta-cyclodextrin (6-mnt-beta-CD), with titanocene (titanocene di[mono[6-deoxy-6-(2-butenedinitrile-2,3-dimercapto)]-beta-cyclodextrin], Cp2Ti[6-mnt-beta-CD]2) has been synthesized and characterized by IR spectroscopy, UV spectroscopy, elemental analysis, thermogravimetry, 1H- and 13C-NMR spectroscopy. The stoichiometry of the target molecule was determined by 1H-NMR spectroscopy and elemental analysis.
- Published
- 2003
- Full Text
- View/download PDF
217. Preparation and electrochemistry of azobenzene self-assembled monolayers on gold--long range tunneling and end-group hydrogen bonding effect.
- Author
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Zhang WW, Li HF, Liu L, Xie JL, Lu CS, Zhou Y, Ren XM, and Meng QJ
- Subjects
- Electrolysis, Hydrogen Bonding, Hydrogen-Ion Concentration, Kinetics, Oxidation-Reduction, Azo Compounds chemistry, Electrochemistry methods, Electrodes, Gold, Surface-Active Agents chemistry
- Abstract
A series of thiol-functionalied azobenzene derivatives (RAzoCnSH: R=H for n=3-6, abbreviated as AzoCnSH; R=CH(3)CONH for n=4, abbreviated as aaAzoC4SH) on gold electrodes were prepared and their self-assembly and electrochemical properties were studied by cyclic voltammetry. They all formed uniform and reproducible self-assembled monolayers (SAMs) on gold and showed well-behaved voltammetric responses in aqueous solution. Both the length of the alkyl chain spacer and the H-bonding of the end acetamino group had effects on the stability and the electrochemical kinetics of the SAMs, and the effect of the H-bonding was dominant. The surface coverage of the SAMs (AzoCnSH) is gradually increased with an increase of the alkyl chain spacer length, whereas the presence of the terminal acetamino group leads to a greater increase of the surface coverage. At a low scan rate, voltammetric responses corresponding to an irreversible two-electron, two-proton reduction/oxidation of the trans-azobenzene redox center were obtained in the range of +300 mV and -800 mV, which exhibited very large peak-to-peak splitting. At a high scan rate of 500 mV/s, two steps of reversible one-electron, one-proton reduction/oxidation corresponding to the cis-isomer in azobenzene-thiol SAMs (n is odd) was clearly observed between +300 and -200 mV. The apparent electron-transfer rate is decreased with increasing distance between the azobenzene redox center and the gold electrode. The existence of the end acetamino group which restricted the conformational change during the redox process also led to a decrease of the standard rate constant, and this restriction effect is more predominant than the distance effect.
- Published
- 2003
- Full Text
- View/download PDF
218. [Operative treatment of pheochromocytoma: report of 11 cases].
- Author
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Wu ZG, Zhou FJ, Qin ZK, Xie JL, and Wang GL
- Subjects
- Adult, Female, Humans, Male, Middle Aged, Treatment Outcome, Pheochromocytoma surgery
- Abstract
Background & Objective: Pheochromocytoma is clinically uncommon. Pheochromocytoma patients without hypertension or symptoms are often misdiagnosed. The patients could be cured by surgical removal of the tumor. However, surgery is dangerous if the preoperative preparation was not well done. The authors reported their experiences in surgical treatment of pheochromocytoma., Methods: Clinical data of 11 patients with pheochromocytoma were reviewed retrospectively to summarize the management of blood pressure during pre-operation and operation., Results: After excision of the tumors, 11 patients have been followed up for 3 months to 10 years. Blood pressure became normal and symptoms vanished in all patients. Only one patient recurred at paraveterbral sympathetic chain 6 months after operation and underwent another operation for removal of the tumor., Conclusion: Surgery is the mainstay of therapy for pheochromocytoma. The adequate preoperative preparation is very important for successful surgery.
- Published
- 2003
219. [Expression and characterization of Kringle 1-4.5 domains of human plasminogen].
- Author
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Zhou QW, Xie JL, Xin L, Xu R, Ye Q, Li ZP, and Gan RB
- Subjects
- Animals, Cattle, Cell Division drug effects, Cell Movement drug effects, Cells, Cultured, Dose-Response Relationship, Drug, Endothelium, Vascular cytology, Endothelium, Vascular drug effects, Fibroblast Growth Factor 2 pharmacology, Gene Expression, Genetic Vectors genetics, Humans, Pichia genetics, Plasminogen chemistry, Recombinant Proteins isolation & purification, Recombinant Proteins pharmacology, Tumor Cells, Cultured, Kringles genetics, Plasminogen genetics
- Abstract
The cDNA encoding Kringle 1-4 and part of Kringle 5 domains of human plasminogen (K1-4.5), obtained from HepG2 by RT-PCR, was cloned into expression vector pHIL-S1. The recombinant plasmid pHIL-K1-4.5 was transformed into Pichi pastoris GS115 and the recombinant yeast was induced to express the recombinant proteins by methanol. The expressed proteins were purified by lysine affinity chromatography to a purity of 95%. The recombinant K1-4.5 inhibited the growth of bovine capillary endothelial cells (BAEC) stimulated by the basic fibroblast growth factor (bFGF), in a dosage-dependent manner with a half maximal concentration of 2 mg/L. rhK1-4.5 also inhibited 40% of the BAEC migration stimulated by bFGF in the concentration of 1 mg/L.
- Published
- 2003
220. Structural, morphological, and magnetic study of nanocrystalline cobalt-nickel-copper particles.
- Author
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Zhang WW, Cao QQ, Xie JL, Ren XM, Lu CS, Zhou Y, Yao YG, and Meng QJ
- Abstract
Nanocrystalline Co(x)Ni(y)Cu(100-x-y) particles were synthesized by the reduction of metal acetates in a mixture of polyol and Tween 80. Inductively coupled plasma (ICP) analysis revealed that the actual wt% of Co, Ni, and Cu in these nanoparticles was nearly the same as in the starting solutions. The structures of the particles were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED) spectroscopy, and vibrating sample magnetometry (VSM). The results of XRD and VSM confirmed that there was no metastable alloying in the particles. The particles were composites, consisting of nanoscale crystallites of face-centered cubic (fcc) Cu, face-centered cubic (fcc) Ni, and face-centered cubic (fcc) Co. During preparation the nucleation of Cu occurred first; then small Cu nuclei acted as cores for the precipitation of Co and Ni. The particles showed an increase in saturation magnetization (M(s)) as the concentration of Co or Ni in the particles was increased. The changes of both M(s) and coercivity of the particles with increasing annealing temperatures were studied. The coercivity of the particles was very high; it could reach as high as 489 Oe for Co34.3Ni31.2Cu34.5) .
- Published
- 2003
- Full Text
- View/download PDF
221. [Discussion of new classification of epididymal malformation in cryptorchidism of children].
- Author
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Zhang DL, Li Z, and Xie JL
- Subjects
- Child, Child, Preschool, Cryptorchidism classification, Cryptorchidism surgery, Humans, Male, Testicular Hydrocele pathology, Cryptorchidism pathology, Epididymis abnormalities
- Abstract
Objectives: To discuss the new classification of epididymal malformation in cryptorchidism., Methods: One hundred and fifty-three boys who were two to four year old underwent orchidopexy for cryptorchidism and 144 hydroceles who were two to eight year old were enrolled into control. Based on the observation of the status between testis and epididymia, the length of epididymis and the configuration of epididymis as well as spermaduct, we divided epididymal malformations in cryptorchidism into three types. Type I: obstruction of sperm transport, including I A, I B and I C; Type II: possible obstruction of sperm transport, defined by II A and II B; Type III: no obstruction of sperm transport, classified into III A and III B., Results: There were 47 (23.4%) out of 201 undescended testes with malformation of epididymides and 16 (10.3%) out of 155 testes in the control(P < 0.05). 11 of 48 cases of bilateral cryptorchidism had the same malformations, with 2 cases of type IB, 2 of IC, 1 of II A, 4 of III A and 2 of IV B underwent orchidopexy or orchiectomy., Conclusions: These data showed that the incidence of epididymal abnormalities in cryptorchidism was higher than that in the hydroceles and it's not essential to have orchiectomy unlimitedly to serious epididymal abnormalities.
- Published
- 2002
222. Self-assembly of L-cysteine-copper(II)/copper(I) multilayer thin films on gold.
- Author
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Zhang WW, Lu CS, Zou Y, Xie JL, Ren XM, Zhu HZ, and Meng QJ
- Abstract
Self-assembled multilayer thin films have been prepared on Au substrate by alternate surface derivatization with L-cysteine hydrochloride and cupric perchlorate. The layer-by-layer structure at each step of multilayer formation was investigated by X-ray photoelectron spectroscopy. The measurements indicate that there are two structure modes in the multilayers. One is that Cu(2+) sandwiches between two amino acid groups. The other one is that Cu(+) is bonded through disulfide and thiolate. This process is also confirmed by cyclic voltammetry of Cu ion at different self-assembled multilayers. Steps further on will lead to repeated multilayer films.
- Published
- 2002
- Full Text
- View/download PDF
223. Photosensitization of hypomycin B--a novel perylenequinonoid pigment with only one intramolecular hydrogen bond.
- Author
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Zhang HY, Liu W, Liu WZ, and Xie JL
- Subjects
- Humans, Hydrogen Bonding, In Vitro Techniques, Oxygen chemistry, Perylene analogs & derivatives, Phenol, Photochemistry, Photochemotherapy, Singlet Oxygen, Spectrometry, Fluorescence, Spectrophotometry, Superoxides chemistry, Perylene chemistry, Perylene radiation effects, Photosensitizing Agents chemistry, Photosensitizing Agents radiation effects, Quinones chemistry, Quinones radiation effects
- Abstract
Electron spin resonance technique and spin-trapping methods were used to determine the photoproduction of 1O2 and O2.- by hypomycin B (HMB), a novel perylenequinonoid pigment (PQP) possessing only one hydroxyl group. It was found that the yields of 1O2 and O2.- for HMB were comparable to those for hypocrellin A, a typical natural PQP with good photosensitivity. In addition, the absorption and fluorescence spectra for HMB were investigated. The pKa values in the ground and excited states of HMB were determined to be 8.94 and 5.54, respectively. Thus, the photodynamic mechanisms of HMB may involve not only the photogeneration of 1O2 and O2.- but also the light-induced acidification. Consequently, HMB is proposed to be a good photodynamic therapeutic agent.
- Published
- 2001
- Full Text
- View/download PDF
224. [Structural elucidation of a new perylenequinone].
- Author
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Liu WZ, Ma LY, Li C, Chen YT, and Xie JL
- Subjects
- Molecular Structure, Perylene chemistry, Quinones chemistry, Hypocreales chemistry, Perylene analogs & derivatives, Perylene isolation & purification, Quinones isolation & purification
- Abstract
Aim: To study the chemical constituents from the mycelia of Hypomyces sp., Methods: Silica gel column chromatography was employed for the isolation and purification. The structure of compound 1 was elucidated on the basis of spectral analysis., Results and Conclusion: A new perylenequinone, named hypomycin B 1, was isolated from the mycelia of Hypomyces sp.
- Published
- 2001
225. Collective efficacy versus self-efficacy in coping responses to stressors and control: a cross-cultural study.
- Author
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Schaubroeck J, Lam SS, and Xie JL
- Subjects
- Adult, Cross-Cultural Comparison, Female, Hong Kong, Humans, Male, Surveys and Questionnaires, United States, Adaptation, Psychological, Culture, Self Efficacy, Stress, Psychological psychology, Work
- Abstract
This study examined how cultural differences and efficacy perceptions influence the role of job control in coping with job demands. Perceiving higher control mitigated the effects of demands on psychological health symptoms and turnover intentions only among American bank tellers reporting high job self-efficacy. Among American tellers reporting low job self-efficacy, perceived control exacerbated the effects of demands. However, in a matched Hong Kong sample, collective efficacy interacted in the same way with control and demands as job self-efficacy had in the American sample. These differences appear to be explained by the individual attributes of idiocentrism and allocentrism that are linked to the societal norms of individualism and collectivism, respectively.
- Published
- 2000
- Full Text
- View/download PDF
226. [The effect of combined treatment of recombinant human growth hormone and insulin-like growth factor-1 on wound healing and protein catabolism in burned rats].
- Author
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Chen HD, Xie JL, and Lai W
- Subjects
- Animals, Burns metabolism, Male, Proteins metabolism, Random Allocation, Rats, Rats, Wistar, Recombinant Proteins therapeutic use, Burns therapy, Growth Substances therapeutic use, Insulin-Like Growth Factor I therapeutic use, Wound Healing drug effects
- Abstract
Objective: To investigate the effect of combined treatment of recombinant human growth hormone (rhGH) and insulin-like growth factor-1 (IGF-1) on wound healing and protein catabolism in burned rats., Methods: Forty Wistar rats with deep II degree scald injury were divided randomly into four groups and received rhGH (0.1 U/kg.d), rhGH (0.1 U/kg.d) plus IGF-1 (2.0 mg/kg.d), IGF-1 (2.0 mg/kg.d) and Ringer's solution (2 ml/kg.d, as control group) respectively. The wound healing time and protein catabolism levels of every groups were compared after 2 weeks., Results: Total body weight began to increase after 2 weeks in rhGH group and rhGH plus IGF-1 group, but in control group, it was occurred after 4-5 weeks. The body weight of rhGH plus IGF-1 group was 1.65 times than that of rhGH group. The wound healing time in rhGH plus IGF-1 group (17.1 +/- 4.4) days was significantly lower than that of rhGH (20.5 +/- 4.8) days and control group (29.7 +/- 6.3) days. The protein level of rhGH plus IGF-1 group was significantly higher than that of control group and rhGH group., Conclusion: It suggests that rhGH plus IGF-1 with synergism is more effective in promoting wound healing and increasing the protein catabolism.
- Published
- 1999
227. Perceptions of absence from work: People's Republic of China versus Canada.
- Author
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Johns G and Xie JL
- Subjects
- Adult, Canada, China, Cross-Cultural Comparison, Female, Humans, Male, Surveys and Questionnaires, Absenteeism, Attitude, Employment
- Abstract
Cross-cultural theory was marshaled to predict how views of absence from work would be similar and different in Canada and the People's Republic of China. Respondents (N = 1,209) from both cultures had self-serving perceptions of their own absence levels, seeing them as exemplary compared with those of their work group and occupational peers. The Chinese showed a stronger tendency to generate estimates that favored their work group. Both cultural groups underreported their own actual absence. Chinese managers and employees agreed on absence norms, whereas Canadian managers provided lower estimates than did employees. Canadians and Chinese ranked the legitimacy of reasons for absence and attendance fairly similarly, but ratings showed that Canadians were less likely to endorse domestic reasons for absence, whereas Chinese were less likely to endorse illness, stress, and depression.
- Published
- 1998
- Full Text
- View/download PDF
228. Chemical modification and immobilization of L-aspartase.
- Author
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Cheng YH, Xie JL, Chui MZ, and Wang SQ
- Subjects
- Escherichia coli enzymology, Kinetics, Ammonia-Lyases metabolism, Aspartate Ammonia-Lyase metabolism, Enzymes, Immobilized metabolism
- Published
- 1987
- Full Text
- View/download PDF
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