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Late gadolinium enhancement (LGE) magnetic resonance imaging (MRI) is valuable for diagnosis and assessment of the severity of various myocardial diseases owing to its potential to visualize myocardial scars. T1 mapping is complementary to LGE because it can quantify the degree of myocardial fibrosis or edema. As such, T1-weighted imaging techniques, including LGE using an inversion recovery sequence, contribute to cardiac MRI. T2-weighted imaging is widely used to characterize the tissue of many organs. T2-weighted imaging is used in cardiac MRI to identify myocardial edema related to chest pain, acute myocardial diseases, or severe myocardial injuries. However, it is difficult to determine the presence and extent of myocardial edema because of the low contrast between normal and diseased myocardium and image artifacts of T2-weighted images and the lack of an established method to quantify the images. T2 mapping quantifies myocardial T2 values and help identify myocardial edema. The T2 values are significantly related to the clinical symptoms or severity of nonischemic cardiomyopathy. Texture analysis is a postprocessing method to quantify tissue alterations that are reflected in the T2-weighted images. Texture analysis provides a variety of parameters, such as skewness, entropy, and grey-scale non-uniformity, without the need for additional sequences. The abnormal signal intensity on T2-weighted images or T2 values may correspond to not only myocardial edema but also other tissue alterations. In this review, the techniques of cardiac T2 mapping and texture analysis and their clinical relevance are described.

BackgroundMeningiomas are the most common benign intracranial tumors, and commonly comprise high-vascularizing but slow-growing tumors. On the other hand, meningiomas arising from the ventricular system are of rare occurrence, and spontaneous hemorrhage is an infrequent event.Case presentationWe describe here the rare clinical manifestations of a 28-year-old female with acute intracranial hemorrhage located in the trigone of the lateral ventricle who was initially thought to have suffered an acute cerebrovascular accident, but was subsequently confirmed to have a benign intraventricular meningioma. To clarify the clinical features of such a rare course of meningioma, we also present a short literature review of acute intracranial hemorrhage caused by intraventricular meningioma.ConclusionsVentricular meningioma presenting with hemorrhage such as acute stroke is a rare event, but recognition of such a pathogenesis is important. Although further accumulation of clinical data is needed, we suggest that early surgery should be undertaken in patients with lateral ventricular meningioma, even if it is not so large or asymptomatic.

Background: Myocardial infarction (MI), a common presentation for cardiovascular disease, is caused by reduction of blood flow and oxygen supply and is one of the main causes of death worldwide. MicroRNAs participate in multiple physiological and pathological processed and play crucial role in myocardial infarction. Results: qRT-PCR analysis showed that expression level of miR-19a-3p was increased in serum of patient with MI. In vitro study indicated that the miR-19a-3p level was upregulated in response to H2O2 treatment and transferred by exosome, and then, uptake occurred in endothelial cells. Furthermore, western blot and immunostaining showed that treatment of exosome enriched miR-19a-3p suppressed the proliferation of endothelial cells and induced cell death, which was inhibited by AMO-19 transfection. Administration of antagomiR-19a-3p promoted angiogenesis and improved heart function of MI mice. Moreover, miR-19a-3p overexpression downregulated the protein level of HIF-1α and transfection of si-HIF-1α reversed the promotion of endothelial cells proliferation caused by AMO-19 transfection. In addition, antagomiR-19a-3p treatment accelerated angiogenesis and infection of AAV5-shHIF-1α inhibited that effect in MI mice. Conclusions: In conclusion, our finding indicated that miR-19a-3p inhibited endothelial cells proliferation and angiogenesis via targeting HIF-1α and attenuated heart function of mice after MI, and suggested a new mechanism of cell-to-cell communication between cardiomyocytes and endothelial cells.

Aims Intercropping cereals with legumes may achieve high crop yields at reduced input levels. Several studies have indicated that intercropping increases phosphorus use efficiency but no overarching analysis exists on the role of species traits and input levels. Here we synthesize the available information on P use efficiency in cereal/legume intercropping. Methods Global data on yields, P uptake and nutrient input in cereal/legume mixtures were extracted from the literature and statistically analyzed. Co-variables explaining P uptake efficiency and yield were considered. Results P uptake was substantially increased with an average value of LERP, the land equivalent ratio for P uptake, of 1.24, and an average NEP (observed P uptake minus expected P uptake) of 3.67 kg P ha−1. The conversion efficiency of P uptake to biomass decreased with P uptake and was lower in intercrops than in sole crops but the conversion efficiency to yield was not affected by intercropping. The P fertilizer requirement was 21% lower in intercrops than in sole crops for the same yields. Conclusions Substantial improvements in land use efficiency and P uptake are obtained by cereal/legume intercropping. Cereal/legume intercropping has therefore potential to increase P fertilizer use efficiency in agriculture.

Rotator cuff tears (RCTs) are a common cause of disability and pain in the adult population. Despite the successful repair of the torn tendon, the delay between the time of injury and time of repair can cause muscle atrophy. The goal of the study was to engineer an electroconductive nanofibrous matrix with an aligned orientation to enhance muscle regeneration after rotator cuff (RC) repair. The electroconductive nanofibrous matrix was fabricated by coating Poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS) nanoparticles onto the aligned poly(ε-caprolactone) (PCL) electrospun nanofibers. The regenerative potential of the matrix was evaluated using two repair models of RCTs include acute and sub-acute. Sprague–Dawley rats (n=39) were randomly assigned to 1 of 8 groups. For the acute model, the matrix was implanted on supraspinatus muscle immediately after the injury. The repair surgery for the sub-acute model was conducted 6 weeks after injury. The supraspinatus muscle was harvested for histological analysis two and six weeks after repair. The results demonstrated the efficacy of electrical and topographical cues on the treatment of muscle atrophy in vivo. In both acute and sub-acute models, the stimulus effects of topographical and electrical cues reduced the gap area between muscle fibers. This study showed that muscle atrophy can be alleviated by successful surgical repair using an electroconductive nanofibrous matrix in a rat RC model.

Phosphorus (P) is the main factor limiting crop production in red soils. Examining the bacterial community harboring phoD gene under different long-term cropping systems may supply meaningful insights for improving soil P availability. Here, we analyzed how the cropping systems affect community structure of phoD-harboring bacteria by changing physicochemical properties in red soils. Twenty-four soil samples from eight cropping systems (abandoned farmland, corn continuous cropping, mango continuous cropping, taro continuous cropping, zucchini–corn rotation, pea–corn rotation, canola–tobacco rotation, and walnut–tobacco intercropping for > 10 years) were collected from 0 to 20 cm depth in Miyi County, Sichuan Province, China. The abundance of phoD gene was determined by quantitative PCR, and phoD-harboring bacterial community structure was analyzed by high-throughput sequencing. Available P concentration and phoD abundance were the highest in pea-corn rotation soil, while alkaline phosphatase (ALP) activity was the lowest in this soil. Available P showed a significant positive relationship with phoD abundance, while a negative relationship with ALP activity. Moreover, the dominant phyla of phoD-harboring bacterial community were Proteobacteria and Actinobacteria, with Cupriavidus and Bradyrhizobium being the dominant genera. The relative abundances of Streptomyces, Pseudomonas, and Amycolatopsis were positively related to soil P concentration (total and available P) and negatively related to ALP activity, indicating that these genera may be essential in the mineralization of soil organic P. Redundancy analysis (RDA) showed that ALP, soil organic carbon (SOC), and N:P ratio were the remarkable factors affecting the structure of phoD-containing bacterial community across all cropping system soils. Meanwhile, structural equation model (SEM) showed that the effects of total N and available P on soil phoD-harboring bacterial community structure were stronger than that of SOC. Further, soil texture was also one of the main factors affecting the phoD-harboring bacterial community structure, which had a notable up-regulation effect on abundance and diversity of phoD gene and indirectly regulate gene composition by affecting available P. The long-term cropping systems can affect the community structure of phoD-containing bacteria and ALP activity by altering physicochemical properties, and thus contribute to regulation of the biogeochemical cycle of P in red soils. The main factors driving the changes in phoD-harboring bacterial community structure were soil texture, SOC, total N, and available P.

The novel coronavirus SARS-CoV-2 is causing a pandemic worldwide. Several months after the initial outbreak, with the help of tight isolation policy, SARS-CoV-2 transmission in the general population is now under control in China. The number of new cases dramatically decreases compared to the outbreak period. Thus, we name this phase the “post-outbreak” period when China has lived through the pandemic phase while many other countries are still facing fast growing cases. To keep the number of new cases at low level under the consistent pressure of imported cases from other countries and prevent a second outbreak in China are tremendous challenges to all workers in the health care system. Currently available studies of the basic reproduction number ( R 0) of SARS-CoV-2 demonstrate that the new virus has a higher transmission capacity than other coronaviruses such as SARS-CoV and MERS-CoV. Besides, longitudinal serological studies illuminate that protective antibodies to SARS-CoV-2 may turn negative at early convalescent phase in certain patients, indicating a relatively short protection time. Recent prediction models of the transmission dynamics suggested a risk of recurrent outbreaks of SARS-CoV-2, even in seasonal or perennial pattern. When autumn and winter are approaching, the number of patients infected with SARS-CoV-2 will be likely to increase if appropriate measures are not taken, which will further challenge the medical system. It has been shown that during the outbreak period, the infection rate of SARS-CoV-2 among medical workers is relatively high. According to the serum specific antibody test, about half of the front-line medical staffs have been infected in some countries. Some cluster cases of SARS-CoV-2 infection in hospital settings have been reported. This indicates that the risk of virus transmission in the hospital environment is high and more intensive infection control is needed. In this article, we particularly focus on several high-risk areas, such as respiratory clinics, eye clinics and obstetrical clinics. We summarize their unique clinical practices and possible risks of virus transmission, and propose some suggestions for the protection of health care workers in daily work during the “post-outbreak” period based on guidelines proposed by international groups and specialists of the fields. The review also discusses some approaches that hospitals can take during the “post-outbreak” period to early detect infected patients and cut nosocomial transmission. We focus on the topics including the prevention of the virus spreading via aerosol between patients and doctors, innovation of fast and reliable diagnostic methods to effectively identify SARS-CoV-2 carriers from large number of suspected patients, implementation of effective remote doctor consultation and establishment of a patient stratification system to reduce cross-infection, etc. This article aims to highlight the awareness of SARS-CoV-2 virus transmission in hospitals and clinics during the “post-outbreak” phase and propose crucial aspects for the research of nosocomial infection control.

This article proposes an LED driver circuit which is wide-range continuous dimmable for high power lighting application. The proposed LED driver is based on single-inductor–multiple-output (SIMO) dc–dc converter. A pulsewidth modulation (PWM) control strategy is applied on the LED driver to control the brightness of LEDs flexibly and precisely, with which the dimming frequency can be increased significantly, and the dimming ratio for all branches can be adjusted separately. PWM dimming would cause the inductor current overshooting when dimming switch alters from off to on , which makes the LED current varying. To suppress the inductor current overshooting, a sampling–holding circuit is adopted in the feedback loop of the proposed LED driver. A prototype of the proposed LED driver is fabricated and tested. The measurement results show that the dimming frequency can be increased to 10 kHz, the dimming ratio can be set from 5% to 100% with a minimum pulsewidth of 5 $\mu$ s, and the inductor current overshooting is suppressed from 150% to approximately 0%. The proposed LED driver can output more than 80 W and achieve a conversion efficiency of 97%.

Endometriosis has a high recurrence rate after treatment, and there are no effective recurrence predictors. LncRNA H19 has been found to be a predictor of the poor prognosis of multiple diseases. Here, we aimed to investigate the expression and clinical implications of lncRNAH19 in endometriosis and explore the clinical application value of lncRNAH19 for recurrence prediction. LncRNA H19 expression was evaluated in 104 ectopic and eutopic endometrial samples from patients with endometriosis and 50 control endometrial samples from patients without endometriosis. The association between lncRNA H19 expression and the clinical characteristics of endometriosis as well as its value as a potential predictor of recurrence were analyzed. LncRNA H19 expression in the ectopic and eutopic endometria of endometriosis patients was significantly higher than that in the normal endometrium. LncRNA H19 expression in the ectopic endometrium was associated with infertility, recurrence, bilateral ovarian lesions, an increased CA125 level, and revised American Fertility Society (rAFS) stage. Multivariate logistic regression analysis showed that age less than 40 years and lncRNA H19 overexpression in the ectopic endometrium were independent prognostic factors of endometriosis recurrence, and the receiver operating characteristic (ROC) curve showed that the sensitivity and specificity for predicting recurrence were 90.9% and 61.0%, respectively, when the lncRNA H19 expression level in the ectopic endometrium was higher than 0.0277. LncRNA H19 may be involved in the pathogenesis of endometriosis especially in the mechanism of recurrence and is a novel potential predictor of the recurrence of endometriosis.

As a promising ultra-wide bandgap semiconductor, the β-phase of Ga 2 O 3 has attracted more and more interest in the field of power electronics due to its ultra-wide bandgap (4.8 eV), high theoretical breakdown electric field (8 MV/cm), and large Baliga's figure of merit, which is deemed as a potential candidate for next generation high-power electronics, including diodes, field effect transistors (FETs), etc. In this article, we introduce the basic material properties of Ga 2 O 3 , and review the recent progress and advances of β-Ga 2 O 3 based metal-oxide-semiconductor field-effect transistors (MOSFETs). Due to the problematic p-type doping technology up to now, the enhancement-mode (E-mode) β-Ga 2 O 3 FETs face more difficulties, compared with depletion mode (D-mode). This article focuses on reviewing the recent progress of E-mode Ga 2 O 3 MOSFETs, summarizing and comparing various feasible solutions when p-type doping is absent. Furthermore, the device fabrication and performances of state-of-art β-Ga 2 O 3 MOSFETs, including D-mode, E-mode, and planar/vertical structure are fully discussed and compared, as well as potential solutions to the challenges of Ga 2 O 3 FETs.

With the deepening of the understanding and research in coronavirus disease 2019 (COVID-19), the diagnosis and treatment of COVID-19 have been constantly updated and improved. In China, since the implementation of "Guidelines for the Diagnosis and Treatment of COVID-19 (1(st) Trial Version)" on Jan. 15, 2020, 2(nd) to 7(th) versions (including revision of 5(th) version) was updated from Jan. 18, Jan. 22, Jan. 27, Feb. 4, Feb. 8, Feb. 18 and Mar. 3, respectively. Versions updated subsequently provide more detailed information in many ways than the 1(st) and 2(nd) versions, so this paper will introduce the development of the main contents of the 3(rd) to 7(th) versions of COVID-19 guidelines in China, which hopes to provide help for clinical medical staff in other countries fighting with this disease.

Weedy rice ( Oryza sativa f. spontanea ) presumably originated from rice but has weedy characteristics. It is one of the malignant weeds in rice field that decreases rice production and grain quality severely. In this study, the biological characteristics of weedy rice were described from morphology, shattering, dormancy and stress tolerance. The process of weedy rice origination from de-domestication of cultivated rice was summarized briefly. We also introduced the worldwide distribution of weedy rice as well as its non-uniform distribution in China with the most severe occurrence in middle-south area of Jiangsu province and Zhanjiang of Guangzhou province. In addition, we analyzed the agricultural damage on rice yield, rice quality and paddy field ecology environment by weedy rice. To control the occurrence and spread of weedy rice, we further proposed comprehensive measures, including prevention and controlling measures, reasonable cultivation system, and scientific use of chemical herbicides.

As the p-type doping $\beta $ -Ga2O3 is absent up to now, metal gate (MG) stacks with high work functions are expected to benefit the fabrication of normally- OFF $\beta$ -Ga2O3 transistors. In this article, the electrical characteristics of $\beta $ -Ga2O3 metal-electrode-gated metal–oxide–semiconductor (MOS) devices with Al-rich HfAlO dielectrics and different MG stacks (Ni, Au, Pt, and Ti) are evaluated. The interface state density ( ${D}_{\text {it}}$ ) of HfAlO/ $\beta $ -Ga2O3 interface is characterized based on the frequency-dependent capacitance–voltage ( ${C}{-}{V}$ ) and photo-assisted deep ultraviolet (DUV) ${C}{-}{V}$ measurements. An average $D_{\text {it}}$ of ${4.45}\times {10}^{{11}}$ eV $^{-{1}}$ cm $^{-{2}}$ is extracted from the photo-assisted (deep UV) ${C}{-}{V}$ measurement, while a large amount of border traps, negative fixed charges, and deep traps is also induced at the oxide layer and/or HfAlO/ $\beta $ -Ga2O3 interface. Then, this article investigates the evaluations of Ti, Ni, Au, and Pt as candidate MGs for $\beta $ -Ga2O3 MOS using Al-rich HfAlO as gate dielectric. The obvious flat-band voltage ( $V_{\text {FB}}$ ) shift and gate leakage variation are observed in $\beta $ -Ga2O3 capacitors with different MG solutions, indicating that HfAlO dielectric combined with Ni, Au, and Pt MGs is promising to facilitate some beneficial modifications of normally- OFF $\beta$ -Ga2O3 transistors, while Ti electrode is more suitable for normally- ON $\beta$ -Ga2O3 transistors. This article provides an additional practical guideline for choosing the appropriate MG stacks and potential gate dielectric to the development of normally-OFF Ga2O3 transistors.

Purpose Circular RNAs (circRNAs) are novel type of noncoding RNAs that play important roles and serve as noninvasive biomarkers in various cancers. In the present study, we focused on circFoxO3a and aimed to investigate its prognostic value as a novel serum biomarker for squamous cervical cancer (SCC). Patients and methods Our study included 103 SCC patients from Obstetrics and Gynecology Hospital of Fudan University. Expression levels of circFoxO3a in the serum of patients with SCC were examined by reverse transcription‑quantitative PCR (RT‑qPCR). The correlation between serum circFoxO3a expression and clinicopathologic factors was analyzed. The Kaplan-Meier method and multivariate Cox regression analysis were applied to evaluate the independent prognostic factors for SCC. A prognostic predictive nomogram was constructed using R software. Results Levels of serum circFoxO3a were decreased in SCC patients compared with controls. Low expression of circFoxO3a was correlated with deeper stromal invasion and positive lymph node metastasis. Moreover, SCC patients with lower expression of serum circFoxO3a showed poorer prognosis, including both overall survival (OS) and recurrence-free survival (RFS). Multivariate Cox analysis indicated low serum circFoxO3a levels to be an unfavorable prognostic factor for both OS and RFS, independent of positive lymph node metastasis. Notably, the predictive nomogram we established further confirmed that serum circFoxO3a is a useful tool for predicting survival in SCC. Conclusion Altogether, our findings demonstrated that serum circFoxO3a could serve as a potential novel noninvasive predictive prognostic biomarker and therapeutic target for SCC.

Lignin-degrading enzymes secreted by white rot fungi play an important role in the degradation of lignin and persistent organic pollutants (POPs). In this study, the effect of environmental C/N on the activity of lignin-degrading enzymes, lignin peroxides (LiP) and manganese peroxidase (MnP), produced by Phanerochaete chrysosporium , a white rot fungus, was investigated. Glucose was used as carbon source and ammonium tartrate of different concentrations as N source to provide different carbon/nitrogen ratio (C/N). The relationships between LiP and MnP activities and environmental C/N were explored. The results showed that the higher the N source concentration, the faster the mycelium pellets aged. The faster the mycelium dry weight increased, the higher the LiP and MnP activities. A high C/N ratio was a necessary condition for the secretion of LiP or MnP. In addition, mycelium dry weight had important influence on enzyme activities. In the 122 C/N treatment, mycelium dry weight had important influence on MnP activity; in the 50 C/N treatment, mycelium dry weight had importance influence on both LiP and MnP.

In this paper, a novel modulation method is proposed for the torque ripple suppression of a brushless DC motor (BLDCM) during both the commutation period and the speed regulation period. The main sources of torque ripple are the imbalance current during commutation and the PWM during speed regulation. A single-inductor multiple-output (SIMO) Buck DC–DC converter is adopted to generate input voltage for the inverter driver of a BLDCM. During the commutation period, the input voltage of an inverter can be adjusted to four times the back electromotive force (EMF), which suppresses the torque ripple caused by imbalance commutation current. Meanwhile, by changing the output voltage of a SIMO DC–DC, the speed of a BLDCM can be regulated without PWM on the inverter switches, which also suppresses the torque ripple. A control algorithm is applied on a Field Programmable Gate Array (FPGA), and the proposed BLDCM drive prototype is fabricated and tested. Measurement results show that with the proposed speed regulation strategy, the torque ripple is suppressed from 33.3% to 20.1%, and that with the proposed suppression strategy in commutation, the torque ripple is suppressed further to 5.3%.

Pollen development is critical to the reproductive success of flowering plants, but how it is regulated is not well understood. Here, we isolated two allelic male-sterile mutants of OsMYB80 and investigated how OsMYB80 regulates male fertility in rice. OsMYB80 was barely expressed in tissues other than anthers, where it initiated the expression during meiosis, reached the peak at the tetrad-releasing stage and then quickly declined afterward. The osmyb80 mutants exhibited premature tapetum cell death, lack of Ubisch bodies, no exine and microspore degeneration. To understand how OsMYB80 regulates anther development, RNA-seq analysis was conducted to identify genes differentially regulated by OsMYB80 in rice anthers. In addition, DNA affinity purification sequencing (DAP-seq) analysis was performed to identify DNA fragments interacting with OsMYB80 in vitro. Overlap of the genes identified by RNA-seq and DAP-seq revealed 188 genes that were differentially regulated by OsMYB80 and also carried an OsMYB80-interacting DNA element in the promoter. Ten of these promoter elements were randomly selected for gel shift assay and yeast one-hybrid assay, and all showed OsMYB80 binding. The 10 promoters also showed OsMYB80-dependent induction when co-expressed in rice protoplast. Functional annotation of the 188 genes suggested that OsMYB80 regulates male fertility by directly targeting multiple biological processes. The identification of these genes significantly enriched the gene networks governing anther development and provided much new information for the understanding of pollen development and male fertility.

Bacterial polyhydroxyalkanoates (PHAs) are a unique class of biodegradable polymers because of their biodegradability in ambient environments and structural diversity enabled by side-chain groups. However, the biosynthesis of PHAs is slow and expensive, limiting their broader applications as commodity plastics. To overcome such limitation, the catalyzed chemical synthesis of bacterial PHAs has been developed, using the metal-catalyzed stereoselective ring-opening (co)polymerization of racemic cyclic diolides (rac-8DLR , R=alkyl group). In this combined experimental and computational study, polymerization kinetics, stereocontrol, copolymerization characteristics, and the properties of the resulting PHAs have been examined. Most notably, stereoselective copolymerizations of rac-8DLMe with rac-8DLR (R=Et, Bu) have yielded high-molecular-weight, crystalline isotactic PHA copolymers that are hard, ductile, and tough plastics, and exhibit polyolefin-like thermal and mechanical properties.

Myosin filament plays a critical role in water-trapping and thermodynamic regulation during processing of brined muscle foods. The redox state and availability of proteolytic/antioxidant enzymes affected by salt may change the ion-binding capacity of myosin consequently contributing to swelling and rehydration. Thus, this study investigated the impact of different salt content (0%, 1%, 2%, 3%, 4%, 5% NaCl) and oxidation in vitro (10 mM H

Robust manufacturing production of natural killer (NK) cells has been challenging in allogeneic NK cell-based therapy. Here, we compared the impact of cytokines on NK cell expansion by developing recombinant K562 feeder cell lines expressing membrane-bound cytokines, mIL15, mIL21, and 41BBL, individually or in combination. We found that 41BBL played a dominant role in promoting up to 500,000-fold of NK cell expansion after a 21-day culture process without inducing exhaustion. However, 41BBL stimulation reduced the overall cytotoxic activity of NK cells when combined with mIL15 and/or mIL21. Additionally, long-term stimulation with mIL15 and/or mIL21, but not 41BBL, increased CD56 expression and the CD56

Wucai (Brassica campestris L.) is a leafy vegetable that originated in China, its soluble sugars accumulate significantly to improve taste quality during maturation, and it is widely accepted by consumers. In this study, we investigated the soluble sugar content at different developmental stages. Two periods including 34 days after planting (DAP) and 46 DAP, which represent the period prior to and after sugar accumulation, respectively, were selected for metabolomic and transcriptomic profiling. Differentially accumulated metabolites (DAMs) were mainly enriched in the pentose phosphate pathway, galactose metabolism, glycolysis/gluconeogenesis, starch and sucrose metabolism, and fructose and mannose metabolism. By orthogonal projection to latent structures-discriminant s-plot (OPLS-DA S-plot) and MetaboAnalyst analyses, D-galactose and β-D-glucose were identified as the major components of sugar accumulation in wucai. Combined with the transcriptome, the pathway of sugar accumulation and the interact network between 26 DEGs and the two sugars were mapped. CWINV4, CEL1, BGLU16, and BraA03g023380.3C had positive correlations with the accumulation of sugar accumulation in wucai. The lower expression of BraA06g003260.3C, BraA08g002960.3C, BraA05g019040.3C, and BraA05g027230.3C promoted sugar accumulation during the ripening of wucai. These findings provide insights into the mechanisms underlying sugar accumulation during commodity maturity, providing a basis for the breeding of sugar-rich wucai cultivars.

Aiming at insufficient suspension force on the impeller when the hydraulic suspension axial flow blood pump is start at low speed, the impeller suspension stability is poor, and can’t quickly enter the suspended working state. By establishing the mathematical model of the suspension force on the impeller, then the influence of the circumferential groove depth of the impeller on the suspension force is analyzed, and the annular groove depth on the impeller blade in the direction of fluid inlet and outlet was determined as (0.26, 0.02 mm). When the blood pump starts, there is an eccentricity between the impeller and the pump tube, the relationship between the suspension force and the speed of the impeller under different eccentricities is analyzed. Combined with the prototype experiment, the circumferential annular grooving design of the impeller can make the blood pump rotate at about 3500 rpm into the suspension state, when the impeller is at 8000 rpm, the impeller can basically achieve stable suspension at the eccentricity of 0.1 mm in the gravity direction, indicating that the reasonable circumferential annular grooving design of the impeller can effectively improve the suspension hydraulic force of the impeller and improve the stability of the hydraulic suspension axial flow blood pump.

Severe acute respiratory syndrome (SARS) is a highly contagious zoonotic disease caused by SARS coronavirus (SARS-CoV). Since its outbreak in Guangdong Province of China in 2002, SARS has caused 8096 infections and 774 deaths by December 31th 2003. Although there have been no more SARS cases reported in human populations since 2004, the recent emergence of a novel coronavirus disease (COVID-19) indicates the potential of the recurrence of SARS and other coronavirus disease among humans. Thus, developing a rapid response SARS vaccine to provide protection for human populations is still needed. Spike (S) protein of SARS-CoV can induce neutralizing antibodies, which is a pivotal immunogenic antigen for vaccine development. Here we constructed a recombinant chimeric vesicular stomatitis virus (VSV) VSVΔG-SARS, in which the glycoprotein (G) gene is replaced with the SARS-CoV S gene. VSVΔG-SARS maintains the bullet-like shape of the native VSV, with the heterogeneous S protein incorporated into its surface instead of G protein. The results of safety trials revealed that VSVΔG-SARS is safe and effective in mice at a dose of 1×106 TCID50. More importantly, only a single-dose immunization of 2×107 TCID50 can provide high-level neutralizing antibodies and robust T cell responses to non-human primate animal models. Thus, our data indicate that VSVΔG-SARS can be used as a rapid response vaccine candidate. Our study on the recombinant VSV-vectored SARS-CoV vaccines can accumulate experience and provide a foundation for the new coronavirus disease in the future.

Increasing soil petroleum hydrocarbons (PHs) pollution have caused world-wide concerns. The removal of PHs from soils mainly involves physical, chemical, biological processes and their combinations. To date, most reviews in this field based on research articles, but limited papers focused on the integration of remediation technologies from the perspective of patents. In this study, 20-years Chinese patents related to the remediation of soil PHs were comprehensively analyzed. It showed an increasing number of patent applications and the patents' quantity were positively correlated with Chinese GDP over the years, suggesting the more the economy developed the more environmental problems and corresponding solutions emerged. In addition, chemical technologies were mostly used in a combination to achieve faster and better effects, while the physical technologies were often used alone due to high costs. In all PHs remediation techniques, bacteria-based bioremediation was the most used from 2000 to 2019. Bacillus spp. and Pseudomonas spp. were the most used bacteria for PHs treatment because these taxa were widely harboring functions such as biosurfactant production and hydrocarbon degradation. The future research on joint technologies combining microbial and physicochemical ones for better remediation effect and application are highly encouraged.

ObjectiveTo establish a radiomics signature and a nomogram model based on enhanced CT images to predict the Ki-67 index of lung cancer.MethodsFrom January 2014 to December 2018, 282 patients with lung cancer who had undergone enhanced CT scans and Ki-67 examination within 2 weeks were retrospectively enrolled and analyzed. The clinical data of the patients were collected, such as age, sex, smoking history, maximum tumor diameter and serum tumor markers. Our primary cohort was randomly divided into a training group (n=197) and a validation group (n=85) at a 7:3 ratio. A Ki-67 index ≤ 40% indicated low expression, and a Ki-67 index > 40% indicated high expression. In total, 396 radiomics features were extracted using AK software. Feature reduction and selection were performed using the lasso regression model. Logistic regression analysis was used to establish a multivariate predictive model to identify high and low Ki-67 expression in lung cancer. A nomogram integrating the radiomics score was established based on multiple logistic regression analysis. Area under the curve (AUC) was used to evaluate the prediction efficiency of the radiomics signature and nomogram.ResultsThe AUC,sensitivity, specificity and accuracy of the radiomics signature in the training and validation groups were 0.88 (95% CI: 0.82~0.93),79.2%,84.3%,81.2% and 0.86 (95% CI: 0.78~0.94),74.6%,88.1%,79.8%, respectively. A nomogram combining radiomics features and clinical risk factors (smoking history and NSE) was developed. The AUC, sensitivity, specificity and accuracy were 0.87 (95% CI: 0.80~0.95), 75.0%, 90.2% and 83.5% in the validation group, respectively.ConclusionThe radiomics signature and nomogram based on enhanced CT images provide a way to predict the Ki-67 expression level in lung cancer.

Photodynamic therapy (PDT) utilizes the photogeneration of reactive oxygen species (ROS) with high cytotoxicity to kill cancer cells, holding great promise for cancer treatment. Fractionated delivery of singlet oxygen (1O2) is a wise approach to relieving hypoxia, thus enhancing the therapeutic efficacy. In this article, an anthracene-functionalized semiconducting compound (DPPA) has been designed and synthesized. With irradiation, the compound is able to undergo efficient intersystem crossing (ISC) and non-radioactive decay for photodynamic/photothermal synergistic therapy. In addition, the anthracene module is able to capture and release 1O2 reversibly with or without irradiation. DPPA nanoparticles (NPs) obtained by nanoprecipitation with DSPE-PEG exhibit considerable high phototoxicity on human kidney cancer cells (A498), and the half maximum inhibitory concentration (IC50) is 15.8 μg/ml. Furthermore, an in vivo study demonstrates that complete tumor suppression was observed when the mice were administered DPPA NPs with the help of laser, compared with the control and dark groups. The H&E analysis of the normal tissues (the heart, liver, spleen, lungs, and kidney) indicates that such NPs cause no side effects, indicating the biosafety of DPPA NPs. The results provide a strategy to design a heavy-atom–free photosensitizer for photothermal and fractionated PDT against kidney tumors.

Vertebral epithelioid hemangioma is a rare vascular tumor composed of the many vessels lined by distinct epithelioid endothelial cells. We present the case of a patient with renal cell cancer (RCC) and suspicious vertebral metastasis presenting with back pain, who was later found to have epithelioid hemangioma. FDG PET/CT demonstrated uptake of FDG not only in RCC, but also in the sixth thoracic vertebral body. The SUVmax of the vertebra was more than twice as high as RCC. This report indicates importance of quantitative assessment of FDG uptake, as well as combined use of MRI.

ROSAH syndrome was recently identified as an autosomal dominant systemic disorder due to mutations in ALPK1. It was characterized by retinal dystrophy, optic nerve edema, splenomegaly, anhidrosis, and migraine headache. We collected and summarized the clinical data of two patients with juvenile onset splenomegaly and oculopathy. Whole exome sequencing (WES) was adapted for genetic analysis. Mutations in ALPK1 were confirmed by Sanger sequencing. Besides juvenile oculopathy and splenomegaly, both patients had intermittent fever and anhidrosis. Patient 2 also experienced recurrent upper respiratory infections in her infancy and developed dental and nail problems in childhood. Elevated TNF-α was their prominent laboratory features. Both patients were found to have a previously reported mutation, c.710C>T, p. T237M (NM_001102406) in ALPK1. Anti-TNF treatment of adalimumab was applied to patient 1, after which her optic disc edema in the left eye continued and the visual acuity deteriorated further. Patient 1 underwent elective splenectomy due to concern for spontaneous rupture of the spleen. Up to date, 18 patients of ROSAH syndrome have been reported. The clinical manifestations were relatively homogeneous, prominently presenting with juvenile onset oculopathy and splenomegaly. As it mainly involves ocular fundus, severe oculopathy deeply affects the quality of life and prognosis of ROSAH patients. Now little has been known about its treatment. As a newly recognized inherited systemic disorder, ROSAH syndrome needs to be paid more attention to, especially for those with juvenile onset splenomegaly and oculopathy.

Soil easily oxidizable organic carbon (EOC) is directly related to CO2 density; dynamics in subsurface EOC have been observed globally in relation to rapid urbanization. However, in the context of rapid urbanization, the factors related to EOC and the response of the EOC pool to urbanization down the profile remain elusive. The aim of the current paper is to investigate possible changes in the distribution of EOC over the soil profile and the impact of land use, socioeconomic, and natural factors on these. The study used samples from 182 soil profiles (0–100 cm) taken in the peri-urban areas of the megacity Chengdu (a typical megacity with rapid urbanization). Main drivers of changes in soil EOC were analyzed by using spatial and regression analyses. Closer to the centre of the city, soil EOC levels were lower and land-use factors and socioeconomic factors contributed more to explaining variation in EOC levels in the 0–40-cm layer, whereas natural factors were most important at larger distance from the city. The effect of land-use factors and socioeconomic factors on EOC reached down to 60-cm depths. Moreover, an estimated 20% loss of EOC stock was observed close to the city in comparison with the surroundings, suggesting that the rapid process of urbanization was accompanied by a loss of EOC stock down the profile to depths of 60 cm, and the negative effects on EOC stock became more intensive as the distance to the city decreased.