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Fucosidosis is a rare lysosomal storage disorder characterized by deficiency of α-L-fucosidase with an autosomal recessive mode of inheritance. Here, we describe a 4-year-old Chinese boy with signs and symptoms of fucosidosis but his parents were phenotypically normal. Whole exome sequencing (WES) identified a novel homozygous single nucleotide deletion (c.82delG) in the exon 1 of the FUCA1 gene. This mutation will lead to a frameshift which will result in the formation of a truncated FUCA1 protein (p.Val28Cysfs*105) of 132 amino acids approximately one-third the size of the wild type FUCA1 protein (466 amino acids). Both parents were carrying the mutation in a heterozygous state. This study expands the mutational spectrum of the FUCA1 gene associated with fucosidosis and emphasises the benefits of WES for accurate and timely clinical diagnosis of this rare disease.

Tianwen-1, the first mission of China’s planetary exploration program, accomplished its goals of orbiting, landing, and roving on the Mars. The entry, descent, and landing (EDL) phase directly determines the success of the entire mission, of which the guidance, navigation, and control (GNC) system is crucial. This paper outlines the Tianwen-1 EDL GNC system design by introducing the GNC requirements followed by presenting the GNC system architecture and algorithms to meet such requirements. The actual flight results for the whole EDL phase are also provided in this paper.

Objective. Gestational diabetes mellitus (GDM) is a common metabolic disorder with onset during pregnancy. However, the etiology and pathogenesis of GDM have not been fully elucidated. In this study, we used a metabolomics approach to investigate the relationship between maternal serum metabolites and GDM in early pregnancy. Methods. A nested case-control study was performed. To establish an early pregnancy cohort, pregnant women in early pregnancy (10‐13+6 weeks) were recruited. In total, 51 patients with GDM and 51 healthy controls were included. Serum samples were analyzed using an untargeted high-performance liquid chromatography mass spectrometry metabolomics approach. The relationships between metabolites and GDM were analyzed by an orthogonal partial least-squares discriminant analysis. Differential metabolites were evaluated using a KEGG pathway analysis. Results. A total of 44 differential metabolites were identified between GDM cases and healthy controls during early pregnancy. Of these, 26 significant metabolites were obtained in early pregnancy after false discovery rate (FDR

Objective. Microinflammation plays a crucial role in podocyte dysfunction in diabetic nephropathy, but its regulatory mechanism is still unclear. This study is aimed at discussing the mechanisms underlying the effect of miRNA-155 on podocyte injury to determine its potential as a therapeutic target. Methods. Cultured immortalized mouse podocytes and diabetic KK-Ay mice models were treated with a miR-155 inhibitor. Western blotting, real-time PCR, ELISA, immunofluorescence, and Luciferase reporter assay were used to analyze markers of inflammation cytokines and podocyte injury. Results. miRNA-155 was found to be highly expressed in serum and kidney tissue of mice with diabetic nephropathy and in cultured podocytes, accompanied by elevated levels of inflammatory factors. Inhibition of miRNA-155 can reduce proteinuria and ACR levels, diminish the secretion of inflammatory molecules, improve kidney function, inhibit podocyte foot fusion, and reverse renal pathological changes in diabetic nephropathy mice. Overexpression of miRNA-155 in vitro can increase inflammatory molecule production in podocytes and aggravates podocyte injury, while miRNA-155 inhibition suppresses inflammatory molecule production in podocytes and reduces podocyte injury. A luciferase assay confirmed that miRNA-155 could selectively bind to 3′-UTR of SIRT1, resulting in decreased SIRT1 expression. In addition, SIRT1 siRNA could offset SIRT1 upregulation and enhance inflammatory factor secretion in podocytes, induced by the miRNA-155 inhibitor. Conclusions. These findings strongly support the hypothesis that miRNA-155 inhibits podocyte inflammation and reduces podocyte injury through SIRT1 silencing. miRNA-155 suppression therapy may be useful for the management of diabetic nephropathy.

This paper establishes a Stackelberg competition model of two universities considering social responsibility (SR) and market sensitivity (MS) and examines the equilibrium results of four different scenarios: only the leader implements SR; only the follower implements SR; both the leader and the follower implement SR; neither the leader nor the follower implements SR. Then, a comparative analysis is carried out on the influence of MS and SR level. The results show that the influence of MS depends on SR implementation instead of competitive position. If MS is sufficiently high, two universities have the motive to implement more SR; under different competition scenarios, universities make different SR decisions, but the leader and the government make the same decisions on the optimal SR level. Therefore, the government could encourage the leader to take SR first.

Background: Mucosal healing (MH) is the key aim of the treat-to-target strategy for patients with Crohn’s disease (CD). The efficacy of infliximab (IFX) on MH in different ileocolonic segments is unclear. The aim of this study was to investigate endoscopic MH in different ileocolonic segments in patients with CD who received IFX treatment. Methods: A retrospective, single-center study was performed in patients with active ileocolonic CD between January 2012 and December 2018. All patients underwent IFX treatment for at least 30 weeks. The MH of five ileocolonic segments was assessed by the Simple Endoscopic Score for CD (SES-CD) at baseline, 14/22 weeks and 30/38 weeks. The SES-CD values were analyzed by a mixed-effects model after the correction for confounding factors. Results: A total of 101 eligible patients were included. The baseline endoscopic severity was similar across segments. At 30/38 weeks, the greatest changes in the SES-CD ulcer size and ulcerated surface subscores were −94.29% and −94.32% both in the transverse colon ( p

The spaceborne Global Navigation Satellite Systems Reflectometry (GNSS-R) offers versatile Earth surface observation. While the accuracy of the computed geometry, required for the implementation of the technique, degrades when Earth’s surface topography is complicated, previous studies ignored the effects of the local terrain surrounding the ideal specular point at a suppositional Earth reference surface. The surface slope and its aspect have been confirmed that it can lead to geolocation-related errors in the traditional radar altimetry, which will be even more intensified in tilt observations. In this study, the effect of large-scale slope on the spaceborne GNSS-R technique is investigated. We propose a new geometry computation strategy based on the property of ellipsoid to carry out forward and inverse calculations of path geometries. Moreover, it can be extended to calculate unusual reflected paths over versatile Earth’s topography by taking the surface slope and aspects into account. A simulation considering the slope effects demonstrates potential errors as large as meters to tens kilometers in geolocation and height estimations in the grazing observation condition over slopes. For validation, a single track over the Greenland surface received by the TechDemoSat 1 (TDS-1) satellite with a slope range from 0% to 1% was processed and analyzed. The results show that using the TanDEM-X 90 m Digital Elevation Model (DEM) as a reference, a slope of 0.6% at an elevation angle of 54 degrees can result in a geolocation inaccuracy of 10 km and a height error of 50 m. The proposed method in this study greatly reduces the standard deviation of geolocations of specular points from 4758 m to 367 m, and height retrievals from 28 m to 5.8 m. Applications associated with topography slopes, e.g., cryosphere could benefit from this method.

Graphitized black pearl (GBP) 2000 supported Pt nanoparticle catalysts is synthesized by a formic acid reduction method. The results of a half-cell accelerated degradation test (ADT) of two protocols and a single-cell ADT show that, Pt/GBP catalyst has excellent stability and durability compared with commercial Pt/C. Especially, the survival time of Pt/GBP-membrane electrode assembly (MEA) reaches 205 min, indicating that it has better reversal tolerance. After the 1003-hour durability test, the proton exchange membrane fuel cell (PEMFC) stack with Pt/GBP presents a slow voltage degradation rate of 5.19% and 36 μV h−1 at 1000 mA cm−2. The durability of the stack is improved because of the durability and stability of the catalyst. In addition, the post morphology characterizations indicate that the structure and particle size of the Pt/GBP catalyst remain unchanged during the dynamic testing protocol, implying its better stability under dynamic load cycles. Therefore, Pt/GBP is a valuable and promising catalyst for PEMFC, and considered as an alternative to classical Pt/C.

The use of lead-free ceramic film materials with positive temperature coefficient of resistivity (PTCR) is widespread in temperature heaters and sensors in micro-electromechanical systems. In this research, the out of plane transport properties of the BiFeO3 (BFO) films have been studied. Surprisingly, PTCR was found in the BFO ceramic films due to the strongly correlated interaction between the multiferroic material BFO and the superconductor YBCO perovskite oxides. To our knowledge, this is the first report on the PTCR effect of BFO films. The BFO/YBCO interface and the bulk conductivity of BFO are important for the PTCR effect, as they make it possible to compare the transport properties of Au/BFO/YBCO- and YBCO/BFO/YBCO-type structures. PTCR was observed in Au/BFO/YBCO at a bias voltage of more than 2 V, but not in the YBCO/BFO/YBCO, even with a 40 V bias voltage. PTCR was found after BFO breakdown of a YBCO/BFO/YBCO capacitor. This indicated that the conductivity of BFO is critical for PTCR. The dependence of PTCR on the superconducting transition temperature illustrates that a cooper-pair can be injected into BFO. Our work presents a method by which to produce a lead-free ceramic film material with PTCR.

Nuclei segmentation is a fundamental but challenging task in histopathological image analysis. One of the main problems is the existence of overlapping regions which increases the difficulty of independent nuclei separation. In this study, to solve the segmentation of nuclei and overlapping regions, we introduce a nuclei segmentation method based on two-stage learning framework consisting of two connected Stacked U-Nets (SUNets). The proposed SUNets consists of four parallel backbone nets, which are merged by the attention generation model. In the first stage, a Stacked U-Net is utilized to predict pixel-wise segmentation of nuclei. The output binary map together with RGB values of the original images are concatenated as the input of the second stage of SUNets. Due to the sizable imbalance of overlapping and background regions, the first network is trained with cross-entropy loss, while the second network is trained with focal loss. We applied the method on two publicly available datasets and achieved state-of-the-art performance for nuclei segmentation–mean Aggregated Jaccard Index (AJI) results were 0.5965 and 0.6210, and F1 scores were 0.8247 and 0.8060, respectively; our method also segmented the overlapping regions between nuclei, with average AJI = 0.3254. The proposed two-stage learning framework outperforms many current segmentation methods, and the consistent good segmentation performance on images from different organs indicates the generalized adaptability of our approach.

Macrophage migration inhibitory factor (MIF) has multiple intrinsic enzymatic activities of the dopachrome/phenylpyruvate tautomerase and thiol protein oxidoreductase, and plays an important role in the development of obesity as a pro-inflammatory cytokine. However, which enzymatic activity of MIF is responsible for regulating in obesity are still unknown. In the present study, we investigated the roles of the tautomerase of MIF in high fat diet (HFD)-induced obesity using MIF tautomerase activity-lacking (MIFP1G/P1G) mice. Our results showed that the serum MIF and the expression of MIF in adipose tissue were increased in HFD-treated mice compared with normal diet fed mice. The bodyweights were significantly reduced in MIFP1G/P1G mice compared with WT mice fed with HFD. The sizes of adipocytes were smaller in MIFP1G/P1G mice compared with WT mice fed with HFD using haematoxylin and eosin (H&E) staining. In addition, the MIFP1G/P1G mice reduced the macrophage infiltration, seen as the decreases of the expression of inflammatory factors such as F4/80, IL-1β, TNFα, MCP1, and IL-6. The glucose tolerance tests (GTT) and insulin tolerance tests (ITT) assays showed that the glucose tolerance and insulin resistance were markedly improved, and the expressions of IRS and PPARγ were upregulated in adipose tissue from MIFP1G/P1G mice fed with HFD. Furthermore, we observed that the expressions of Bax, a pro-apoptotic protein, and the cleaved caspase 3-positive cells in white tissues were decreased and the ratio of Bcl2/Bax was increased in MIFP1G/P1G mice compared with WT mice. Taken together, our results demonstrated that the tautomerase activity-lacking of MIF significantly alleviated the HFD-induced obesity and adipose tissue inflammation, and improved insulin resistance in MIFP1G/P1G mice.

Background. Sepsis is a common complication of acute cholangitis (AC), which is associated with a high mortality rate. Our study is aimed at exploring the significance of white blood cell (WBC), C-reactive protein (CRP), procalcitonin (PCT), soluble triggering receptor expressed on myeloid cells 1 (sTREM-1), and temperature (T) alone or combined together in early identification and curative effect monitoring of AC with or without sepsis. Methods. 65 consecutive cases with AC and 76 control cases were enrolled. They were divided into three groups: Group A (AC with sepsis), Group B (AC without sepsis), and Group C (inpatients without AC or other infections). The levels of WBC, CRP, PCT, sTREM-1, and temperature were measured dynamically. The study was carried out and reported according to STARD 2015 reporting guidelines. Results. CRP had the highest AUC to identify AC from individuals without AC or other infections (AUC 1.000, sensitivity 100.0%, specificity 100.0%, positive predictive value 100.0%, and negative predictive value 100.0%). Among various single indexes, PCT performed best (AUC 0.785, sensitivity 75.8%, specificity 72.2%, positive predictive value 68.7%, and negative predictive value 78.8%) to distinguish sepsis with AC, while different combinations of indexes did not perform better. From day 1 to day 5 of hospitalization, the levels of sTREM-1 in Group A were the highest, followed by Groups B and C (P

To improve the accuracy of the multiple channel integration quality (MCIQ) evaluation, this paper proposes a comprehensive evaluation method using the nonlinear autoregressive exogenous model (NARX) and constructs an index system. First, the entropy method is used to determine the objective weight of each indicator. The indicators used in this paper are process consistency, information consistency, emotional value, procedural value, service structure transparency, online result value, business relevance, and online purchase intention. Second, an improved gray relational analysis (GRA) algorithm is used to obtain the comprehensive gray relational degree between the above eight indicators’ standard samples and the tested samples. Then, this study uses the dataset preprocessed with the GRA algorithm for training the NARX model. Then, this study uses the trained model to evaluate the quality of multiple channel integration comprehensively. Next, this study uses standardized methods to quantify the evaluation results to provide new ideas and theoretical guidance for teaching traditional retailers to use the advantages of multiple channels to expand their online business. This paper uses 50,000 consecutive samples of a product for 3 months as a dataset in the experimental part. Through the GRA method and the NARX model, the comprehensive gray relational degree between the test sample and the ideal sample is obtained, and the results are quantified. Experiments show that, compared with the GRA method, this paper’s method has a higher degree of fit between the output value and the target value.

This paper aims at the phenomenon of the fractured rock column in underground engineering which is prone to collapse when subjected to ground pressure. Uniaxial compression test and particle flow code (PFC2D) are used to analyze the influence of crack dip angle on the mechanical properties, crack propagation, and the failure mode of red sandstone. From the results, it is observed that the stress–strain curve of the precracked red sandstone can be divided into five stages, and there is a critical stress value in the stage of accelerated crack propagation and unstable fracture of the rock sample. Further, the peak strength, maximum strain, and the elastic modulus of the precracked red sandstone increase with the increase of crack dip angle, and the ultimate failure mode of rock sample changes from the “ladder” type failure to slope uneven failure. Furthermore, from PFC2D simulation, it is found that the tensile microcracks contribute more towards the failure of rock samples than the shear cracks. The contact force chain is very weak at the places where the precracks and macroshear planes are formed. This indicates that the original contact force is weakened due to particle fracture. Therefore, the bearing capacity of the precracked rock samples decreases with the increase in load. From the simulation results, it is found that the displacement at the shear plane of the rock sample is large, and the shear dilatation occurs. With the increase in load, the specimen falls off and is ejected. This is due to the weakening of the contact force between the internal particles. Thereafter, it fractures to produce microcracks, which gradually converge, thus providing a prerequisite for the transformation of elastic strain energy into kinetic energy.

The renal and hepatic clearance rates of organophosphate esters (OPEs) in humans were estimated. Six OPEs and their corresponding diester metabolites (mOPEs) were quantified respectively in 30 paired human plasma and urine samples collected in Hengshui, Hebei province, China. The renal clearance rate (CLrenal) of triphenyl phosphate (TPHP), tris(chloroethyl) phosphate (TCEP) and tris(1,3-dichloro-isopropyl) phosphate (TDCIPP) was estimated to be 68.9, 50.9 and 33.3 mL/kg/day, respectively, while it was not calculated for other three OPEs due to the low detection frequencies in human samples. To estimate the clearance rates of the target OPEs, hepatic clearance rates (CLh) of OPEs were extrapolated from their in vitro intrinsic clearance data in human liver microsomes (CLHLM). The calculated CLh values of TCEP and TDCIPP were comparable to their CLrenal, indicating that the in vitro extrapolation method was suitable for estimating the clearance rates of OPEs. The higher binding affinity of TDCIPP with plasma proteins could reduce its renal clearance. The estimated half-lives of Cl-OPEs in human were longer than those of the aryl- and alkyl-OPEs. This study provided a feasible in vitro method to predict the clearance and half-lives of OPEs in human, which is significant for their accurate health risk assessment. Keywords: Organophosphate esters, Organophosphate diester metabolites, Renal clearance, Hepatic clearance, Half-life

DNA phosphorothioation (PT-DNA) is a DNA backbone sulfur modification that is recognized by the type-IV restriction endonuclease ScoMcrA. Here the authors provide insights into sulfur recognition by solving the crystal structure of the PT-DNA bound sulfur-binding domain (SBD) from ScoMcrA and they further show that SBD homologs are widely spread among prokaryotes.

Eradication of human immunodeficiency virus 1 (HIV-1) from an infected individual cannot be achieved using current antiretroviral therapy (ART) regimens. Viral reservoirs established in early infection remain unaffected by ART and are able to replenish systemic infection upon treatment interruption. Simian immunodeficiency virus (SIV) infected macaque models are useful for studying HIV pathogenesis, treatments, and persistent viral reservoirs. Here, we used the SIV macaque model to examine and quantify RNA and DNA positive cells in tissues from macaques that control viral replication (controllers) and those that have persistently high plasma viremia (progressors). A positive correlation was detected between tissue RNA+ cells and plasma viral load in both mesenteric lymph node (LN) and spleen. Similarly, a positive correlation also observed between DNA+ cells and plasma viral load in ileum and jejunum. Controllers had a lower frequency of both RNA and DNA+ cells in several tissues compared to progressors. However, DNA+ cells were prevalent in mesenteric LN, inguinal LN, colon, midbrain, and bone marrow tissues in both controller and progressors. Organized lymphoid tissues of LNs, spleen, and intestine were found as the major tissues positive for virus. Viral RNA and DNA positive cells were detected in brain and thymus in macaques with high plasma viremia and SIV-encephalitis. Both T cells and macrophages were shown to be infected in several tissues, indicating vaccines and ART should be specifically designed to protect these cells in organized lymphoid tissues. These results indicate ART should target infected cells in secondary lymphoid organs to reduce both productively and latently infected cells.

Background/Aims: Lymphangiogenesis plays an important role in the pathogenesis of inflammatory bowel diseases (IBD), and vascular endothelial growth factor-C (VEGF-C) is a powerful lymphangiogenic factor. Adipose-derived stem cells (ADSCs) are a promising therapeutic modality for several diseases because ADSCs secret growth factors and exosomes, which modulate hostile microenvironments affected by diseases. However, the effect of exosomes on VEGF-C-dependent lymphangiogenesis and its mechanism remain unclear. Methods: ADSCs were cultured in media with or without recombinant VEGF-C and exosomes were extracted from conditioned medium (CM). Lymphatic endothelial cells (LECs) were treated with ADSCs-derived exosomes, then proliferation, migration and tube formation of LECs were assayed using cell counting Kit-8 (CCK-8), transwell chamber inserts and matrigel-based tube formation assay respectively. Results: We identified significantly higher levels of miR-132 in exosomes isolated from VEGF-C-treated ADSCs (ADSCs/VEGF-C) than in those from ADSCs control. miR-132 was directly transferred from ADSCs to the LECs by the mediation of exosomes. The exosomes from ADSCs/VEGF-C promoted LECs proliferation, migration, and tube formation more potently than the exosomes from ADSCs, whereas pretreatment of ADSCs with miR-132 inhibitor attenuates VEGF-C-dependent lymphangiogenic response. Finally we reveal that miR-132 promotes lymphangiogenic response by directly targeting Smad-7 and regulating TGF-β/Smad signaling. Conclusion: These data provide new insights into the role of ADSCs-derived exosomes as an important player in VEGF-C-dependent lymphangiogenesis.

The reservoir structural fractures have excellent fractal characteristics, as well as self-similarities. Based on the fractal theory, the surface fractal characteristics of faults and the fractal characteristic of fractures in the core of the Xujiahe Formation in the Fault-Fold Belt of the central Yuanba area were studied, and a quantitative relationship was set up between them. Based on the fractal characteristics of faults, predictions were made of the favorable fracture zone, which provides a new idea for the research of fracture, as well as offers theoretical references for exploring the fracture development zone during oil-gas exploration. The results show the following: the seismic value of reflection fault fractal dimension of the Xujiahe Formation is 1.5284; the correlation coefficient R2 is bigger than 0.9901; the capacity dimension linear regression correlation coefficient of the fracture in core of the Xujiahe Formation is bigger than 0.98; the fractal dimension D can well reflect the fault and fracture development degree, as well as the complexity of the fracture system; it can quantitatively calculate the density of the fracture of the reservoir in the area; the areas of capacity dimension bigger than 1.45 are the fracture development zones in the Fault-Fold Belt of the central Yuanba area; the oil and gas enrichment degree is high; the areas with the fractal dimension value between 0.95 and 1.45 are the fracture relatively-developed zones; the fractal dimension with values smaller than 0.95 are the lack of fracture areas.

Novel cathode design holds the key to enabling high performance lithium-sulfur batteries. Here the authors utilize anthraquinone to chemically stabilize polysulfides, revealing that the keto groups of anthraquinone play a critical role in forming strong Lewis acid-based chemical bonding.

Abstract Background Vibrios are among the most diverse and ecologically important marine bacteria, which have evolved many characteristics and lifestyles to occupy various niches. The relationship between genome features and environmental adaptation strategies is an essential part for understanding the ecological functions of vibrios in the marine system. The advent of complete genome sequencing technology has provided an important method of examining the genetic characteristics of vibrios on the genomic level. Results Two Vibrio genomes were sequenced and found to occupy many unique orthologues families which absent from the previously genes pool of the complete genomes of vibrios. Comparative genomics analysis found vibrios encompass a steady core-genome and tremendous pan-genome with substantial gene gain and horizontal gene transfer events in the evolutionary history. Evolutionary analysis based on the core-genome tree suggested that V. fischeri emerged ~ 385 million years ago, along with the occurrence of cephalopods and the flourish of fish. The relatively large genomes, the high number of 16S rRNA gene copies, and the presence of R-M systems and CRISPR system help vibrios live in various marine environments. Chitin-degrading related genes are carried in nearly all the Vibrio genomes. The number of chitinase genes in vibrios has been extremely expanded compared to which in the most recent ancestor of the genus. The chitinase A genes were estimated to have evolved along with the genus, and have undergone significant purifying selective force to conserve the ancestral state. Conclusions Vibrios have experienced extremely genome expansion events during their evolutionary history, allowing them to develop various functions to spread globally. Despite their close phylogenetic relationships, vibrios were found to have a tremendous pan-genome with a steady core-genome, which indicates the highly plastic genome of the genus. Additionally, the existence of various chitin-degrading related genes and the expansion of chitinase A in the genus demonstrate the importance of the chitin utilization for vibrios. Defensive systems in the Vibrio genomes may protect them from the invasion of external DNA. These genomic features investigated here provide a better knowledge of how the evolutionary process has forged Vibrio genomes to occupy various niches.

In this paper, we report on the theoretical and numerical study of the transmission of circularly polarized vortex beams through a plasmonic coaxial nanoring. We show that the transmission peak wavelength of an incident circularly polarized vortex beam is dominantly governed by the total angular momentum, which determines the coupling to the plasmonic eigenmode supported by the coaxial nanoring with a given geometry and ultimately the transmitted beam in the far field. In addition, our study shows that the total angular momentum of the incident circularly polarized vortex beam can be conserved, where the far field of the transmitted beam contains both left- and right-handed circular polarization components with correspondingly modulated orbital angular momentum preserving the same total angular momentum. Our work can be potentially useful to advanced nanophotonic devices harnessing light's angular momentum division.

Sea-level monitoring is important for the safety of coastal cities and analysis of ocean and climate. Sea levels can be estimated based using the global navigation satellite system–interferometry reflectometry (GNSS–IR). The frequency in a signal-to-noise ratio (SNR) arc has been found to be related to the height between the GNSS antenna and reflecting surface, which is called reflector height (RH, h). The height variation of the reflecting surface causes an error, and this error is the most significant error in the GNSS–IR sea-level retrieval. The key to the correction of height variation error lies in the determination of the RH variation rate h˙. The classical correction method determines h˙ based on tide analysis of a coarse RH series over a longer time period. Therefore, h˙ inherits errors in coarse RH series, which contains significant bias during a storm surge, and correcting this requires data accumulation. This study proposes a correction method of height variation error based on just one SNR arc based on wavelet analysis and least-square estimation. First, using wavelet analysis, instantaneous frequencies are extracted in one SNR arc; these frequencies are then converted to RH series. Second, using least-square estimation, h and h˙ are conjointly solved based on the RH series from wavelet analysis. Data of GNSS site HKQT located in Hong Kong, China, during a period of time that includes Typhoon Hato were used. The root-mean-square errors (RMSEs) of retrievals were 21.5 cm for L1, 9.5 cm for L2P, 9.3 cm for L2C, and 7.6 cm for L5 of GPS; 16.8 cm for L1C, 14.1 cm for L1P, 12.6 cm for L2C, and 10.7 cm for L2P of GLONASS; 15.7 cm for L1, 11.2 cm for L5, 12.2 cm for L7, and 9.6 cm for L8 of Galileo. Results showed this method can correct the height variation error based on just one SNR arc, can avoid the inheritance of errors, and can be used during periods of storm surge.

Crocetin is the main component of saffron and exhibits anti-oxidative and anti-inflammatory effects. Neuroinflammation and oxidative stress have been recognized to play a crucial role in the pathogenesis of neuropathic pain. We investigated the effect of crocetin in a mouse model with neuropathic pain induced by spared nerve injury (SNI). Crocetin was intrathecally perfused at various doses for up to 12 days starting 3 days before the surgery. Behavioral tests were performed to determine pain sensitivity. The concentrations of proinflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β) were measured to assess neuroinflammation. In addition, the enzymatic activity of superoxide dismutase (SOD) was measured to reveal the oxidative stress level. We found that repeated treatment with crocetin dose-dependently attenuated mechanical and thermal allodynia in SNI mice. In addition, treatment with high dose of crocetin reduced SNI-induced increase of TNF-α and IL-1β. Crocetin also restored the activity of mitochondrial MnSOD which was reduced in the sciatic nerve and the spinal cord of SNI mice. Collectively, our data demonstrate that crocetin effectively attenuates the neuropathic pain and significantly suppresses oxidative stress and neuroinflammation in the SNI mouse model, supporting the potential of crocetin in the treatment against neuropathic pain.

The HIV reservoir size in target CD4+ T cells during primary infection remains unknown. Here, we sorted peripheral and intestinal CD4+ T cells and quantified the levels of cell-associated SIV RNA and DNA in rhesus macaques within days of SIVmac251 inoculation. As a major target cell of HIV/SIV, CD4+ T cells in both tissues contained a large amount of SIV RNA and DNA at day 8–13 post-SIV infection, in which productive SIV RNA highly correlated with the levels of cell-associated SIV DNA. Memory CD4+ T cells had much higher viral RNA and DNA than naïve subsets, yet memory CD4+ T cells co-expressing CCR5 had no significant reservoir size compared with those that were CCR5-negative in blood and intestine. Collectively, memory CD4+ T cells appear to be the major targets for primary infection, and viral reservoirs are equally distributed in systemic and lymphoid compartments in acutely SIV-infected macaques.

It is well known that the measurement of cloud top height (CTH) is important, and a geostationary satellite is an important measurement method. However, it is difficult for a single geostationary satellite to observe the global CTH, so joint observation by multiple satellites is imperative. We used both active and passive sensors to evaluate the reliability of joint observation of geostationary satellites, which includes consistency and accuracy. We analyzed the error of CTH of FY-4A and HIMAWARI-8 and the consistency between the two satellites and conducted research on the problem of missing measurement (Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO) has CTH data, but FY-4A/HIMAWARI-8 does not) of the two satellites. The results show that FY-4A and HIMAWARI-8 have good consistency and can be jointly observed, but the measurement of CTH of FY-4A and HIMAWARI-8 has large errors, and the error of FY-4A is greater than that of HIMAWIRI-8. The error of CTH is affected by the CTH, cloud optical thickness (COT) and cloud type, and the consistency between the two satellites is mainly affected by the cloud type. FY-4A and HIMAWARI-8 have the problem of missing measurement. The missing rate of HIMAWARI-8 is greater than that of FY-4A, and the missing rate is not affected by the CTH, COT and surface type. Therefore, although FY-4A and HIMAWARI-8 have good consistency, the error of CTH and the problem of missing measurement still limit the reliability of their joint observation.

Terrestrial water storage (TWS) variations are a result of the interconnected impact of various variables including climate, hydrology, ecology, and anthropogenic activities. Previous studies have indicated that climate factors (e.g., precipitation and potential evapotranspiration), vegetation restoration, and water withdrawals (irrigational and industrial water use) are the major determinants of TWS depletion across the Yellow River Basin (YRB). However, few studies have provided explicit information about the main forcing variables that determine spatiotemporal variations in TWS and the synergies among these factors. This study explored the explicit understanding of hydro-climatic and socio-ecological determinants and the key interacting processes that affected the TWS variations across the Yellow River Basin in northern China. The multivariate adaptive regression splines model was employed to establish the relationship function of the long-term trends for the dependent (TWS) and independent (explanatory) variables consisting of normalized difference vegetation index (NDVI), hydro-climate, and human water withdrawal. The long-term trends estimated from the MARS model reproduced the ones calculated by Gravity Recovery and Climate Experiment gravity satellites, with a determination coefficient (R2) of 0.83 and a mean absolute error (MAE) of 1.2 mm. The results showed that precipitation, minimum temperature, runoff, base flow, water withdrawal for electricity, and NDVI were the main drivers of the spatiotemporal variations in the TWS, of which minimum temperature and runoff played a considerable role in TWS variations through the interplay with other variables. The critical values of the trend for interactive variables, which could alter the acting direction of the synergy on the TWS, were also estimated. In view of the connotation of interactive variables, we suggested that spatiotemporal variations in TWS resulted from the coupling of the hydrological energy system, hydrological ecosystem, and hydrological system in the YRB, of which the hydrological system plays the most significant role, followed by the hydrological ecosystem.

Among the efforts to improve the performances of microlasers, optimization of the gain properties and cavity parameters of these lasers has attracted significant attention recently. Distributed feedback lasers, as one of the most promising candidate technologies for electrically pumped microlasers, can be combined with dual-gratings. This combination provides additional freedom for the design of the laser cavity. Here, a holographic dual-grating is designed to improve the distributed feedback laser performance. The holographic dual-grating laser consists of a colloidal quantum dot film with two parallel gratings, comprising first-order (210 nm) and second-order (420 nm) gratings that can be fabricated easily using a combination of spin coating and interference lithography. The feedback and the output from the cavity are controlled using the first-order grating and the second-order grating, respectively. Through careful design and analysis of the dual-grating, a balance is achieved between the feedback and the cavity output such that the lasing threshold based on the dual-grating is nearly half the threshold of conventional distributed feedback lasers. Additionally, the holographic dual-grating laser shows a high level of stability because of the high stability of the colloidal quantum dots against photobleaching.

The value chain comprises several factors and activities useful for strengthening production and distribution by connecting producers with suppliers, intermediaries, and marketplaces and collaboratively creating added value for products or goods. However, the values of agricultural products mostly depend on various factors and actors, which should be linked together for fostering added values. Thus, there may be strong ground for facilitating a smooth transition of the agricultural value chain (AVC) within the prospects of emerging countries. It could be a key means of promoting a profound connection between smallholder farmers and modern agriculture facilities. It could be especially crucial for the highly perishable and high-value product such as fruits. The main aims of the study are to evaluate the factors influencing smallholder apple farmers’ participation in the agricultural value chain and determine whether participation in AVC improves farmers’ production performance. The empirical setup of the study was chosen based on survey data of apple growers in Shandong, China. The propensity score matching (PSM) and inverse probability weighted regression adjustment (IPWRA) models were employed to craft the study’s outcomes. The main conclusions are as follows. (1) Fruit farmers’ gender, total household expenditure, housing value, planting scale, planting years, degree of specialization, days of family labour input, and total days of employment have significant effects on their participation in AVC activities. (2) Fruit farmers’ usage of improved fertilizers and organization participation supports a higher yield and net income per acre. (3) Participating in two kinds of AVC can significantly improve the yield per acre and net income per acre compared with only using one type of AVC (improved fertilizer). Policy makers should improve the existing policy by eliminating institutional barriers and enhancing human factors for farmers to participate in high-value chain activities. Governments should extend technical support, and enhance training facilities, and comprehensively promote the AVC among smallholder farmers. Finally, farmers’ organizations (e.g., cooperatives and credit organizations) should come forward to help facilitate the effectiveness of AVC.

Environmental pollution caused by herb residues and the huge waste of medicinal ingredients contained in herb residues hinder the development of traditional Chinese medicine enterprises. To solve this problem, several probiotics were tested, and Lactobacillus plantarum (HM218749) was finally selected for the reuse of herb residues of Jianweixiaoshi tablets. A mouse model of Helicobacter pylori infection was developed to evaluate the anti-H. pylori infection activity of the herb residue fermentation supernatant using a urease activity test, histological imaging, an enzyme-linked immunosorbent assay (ELISA) and denaturing gel gradient electrophoresis (DGGE). The results demonstrated that the herb residue fermentation supernatant successfully inhibited urease activity, slowed cell infiltration in the gastric area and significantly reduced the production of interleukin-6 (IL-6), IL-8 and TNF-α in the treatment group (p

Abstract Background Myopic foveoschisis (MF) is among the leading causes of visual loss in high myopia. However, it remains controversial whether internal limiting membrane (ILM) peeling or gas tamponade is necessary treatment option for MF. Methods PubMed, EMBASE, CBM, CNKI, WANFANG DATA and VIP databases were systematically reviewed. Outcome indicators were myopic foveoschisis resolution rate, visual acuity improvement and postoperative complications. Results Nine studies that included 239 eyes were selected. The proportion of resolution of foveoschisis was higher in ILM peeling group than non-ILM peeling group (OR = 2.15, 95% CI: 1.06–4.35; P = 0.03). The proportion of postoperative complications was higher in Tamponade group than non-Tamponade group (OR = 10.81, 95% CI: 1.26–93.02; P = 0.03). However, the proportion of visual acuity improvement (OR = 1.63, 95% CI: 0.56–4.80; P = 0.37) between ILM peeling group and non-ILM peeling group and the proportion of resolution of foveoschisis (OR = 1.80, 95% CI: 0.76–4.28; P = 0.18) between Tamponade group and non-Tamponade group were similar. Conclusions Vitrectomy with internal limiting membrane peeling could contribute to better resolution of myopic foveoschisis than non-peeling, however it does not significantly influence the proportion of visual acuity improvement and postoperative complications. Vitrectomy with gas tamponade is associated with more complications than non-tamponade and does not significantly influence the proportion of visual acuity improvement and resolution of myopic foveoschisis.

The microbubble ozonation was used to treat acid red 3R wastewater in order to investigate the influencing factors on its performance. The effects of ozone dose, initial acid red 3R concentration and activated carbon on the performance of microbubble ozonation treatment of acid red 3R wastewater are investigated. The decolorization rate, TOC removal rate, pH variation and ozone utilization efficiency in the microbubble ozonation treatment are compared under different treatment conditions. The results indicate that when increasing ozone dose or decreasing initial acid red 3R concentration, both decolorization rate and TOC removal rate of acid red 3R wastewater increase, but ozone utilization efficiency decreases. The coal-based activated carbon shows strong catalytic activity for microbubble ozonation, which could enhance the decolorization rate and TOC removal rate of acid red 3R wastewater. The better performance of microbubble ozonation treatment is achieved when the ozone dose is 48.3 mg/min and the initial acid red 3R mass concentration is 100 mg/L. Under these conditions, the decolorization efficiency reaches to 100% after treatment for 30 min, the TOC removal efficiency reaches to 78.0% after treatment for 120 min, the reaction rate constant of TOC removal is 0.015 min-1 and the ozone utilization efficiency is higher than 99%. With addition of the coal-based activated carbon of 5 g/L, the decolorization efficiency reaches to 100% after treatment for 15 min, the TOC removal efficiency reaches to 91.2% after treatment for 120 min and the reaction rate constant of TOC removal increases to 0037 min-1.The accumulation and following degradation of intermediate products of small molecule organic acid happens during treatment process, and as a result, the solution pH decreases initially and then increases. Therefore, the optimization of influencing factors for microbubble ozonation could increase both contaminant removal rate and ozone utilization efficiency, and then improve treatment performance significantly.

A series of La2O3-modified CuO-ZnO-ZrO2/HZSM-5 catalysts were prepared by an oxalate co-precipitation method. The catalysts were fully characterized by X-ray diffraction (XRD), N2 adsorption-desorption, hydrogen temperature pro-grammed reduction (H2-TPR), ammonia temperature programmed desorption (NH3-TPD), and X-ray photoelectron spectroscopy (XPS) techniques. The effect of the La2O3 content on the structure and performance of the catalysts was thoroughly investigated. The catalysts were evaluated for the direct synthesis of dimethyl ether (DME) from CO2 hydrogenation. The results displayed that La2O3 addition enhanced catalytic performance, and the maximal CO2 conversion (34.3%) and DME selectivity (57.3%) were obtained over the catalyst with 1% La2O3, which due to the smaller size of Cu species and a larger ratio of Cu+/Cu.

ABSTRACT The demand for a prophylactic vaccine against methicillin-resistant Staphylococcus aureus (MRSA) has motivated numerous dedicated research groups to design and develop such a vaccine. In this study, we have developed a multivalent vaccine, Sta-V5, composed of five conserved antigens involved in three important virulence mechanisms. This prototype vaccine conferred up to 100% protection against multiple epidemiologically relevant S. aureus isolates in five different murine disease models. The vaccine not only elicits functional antibodies that mediate opsonophagocytic killing of S. aureus but also mounts robust antigen-specific T-cell responses. In addition, our data implied that γδ T cells contribute to the protection induced by Sta-V5 in a murine skin infection model. IMPORTANCE Staphylococcus aureus infections, especially MRSA infections, are becoming a major global health issue and are resulting in mortality rates that are increasing every year. However, an effective vaccine is lacking due to the complexity of the infection process of S. aureus. In this study, we found that the addition of two novel protein components to three well-studied vaccine candidates significantly improved the efficacy of the combined vaccine. Furthermore, the five-component vaccine not only elicits a robust antibody response but also induces cytokine secretion by T cells, making it a promising vaccine candidate to fill the void.

Function annotation efforts provide a foundation to our understanding of cellular processes and the functioning of the living cell. This motivates high-throughput computational methods to characterize new protein members of a particular function. Research work has focused on discriminative machine-learning methods, which promise to make efficient, de novo predictions of protein function. Furthermore, available function annotation exists predominantly for individual proteins rather than residues of which only a subset is necessary for the conveyance of a particular function. This limits discriminative approaches to predicting functions for which there is sufficient residue-level annotation, e.g., identification of DNA-binding proteins or where an excellent global representation can be divined. Complete understanding of the various functions of proteins requires discovery and functional annotation at the residue level. Herein, we cast this problem into the setting of multiple-instance learning, which only requires knowledge of the protein’s function yet identifies functionally relevant residues and need not rely on homology. We developed a new multiple-instance leaning algorithm derived from AdaBoost and benchmarked this algorithm against two well-studied protein function prediction tasks: annotating proteins that bind DNA and RNA. This algorithm outperforms certain previous approaches in annotating protein function while identifying functionally relevant residues involved in binding both DNA and RNA, and on one protein-DNA benchmark, it achieves near perfect classification.

The roles of histone demethylation in the regulation of plant flowering, disease resistance, rhythmical response, and seed germination have been elucidated recently; however, how histone demethylation affects leaf senescence remains largely unclear. In this study, we exploited yeast one-hybrid (Y1H) to screen for the upstream regulators of NONYELLOWING1 (NYE1), and identified RELATIVE OF EARLY FLOWERING6 (REF6), a histone H3 lysine 27 tri-methylation (H3K27me3) demethylase, as a putative binding protein of NYE1 promoter. By in vivo and in vitro analyses, we demonstrated that REF6 directly binds to the motif CTCGYTY in NYE1/2 promoters through its zinc finger domain and positively regulates their expression. Loss-of-function of REF6 delayed chlorophyll (Chl) degradation, whereas overexpression of REF6 accelerated Chl degradation. Subsequently, we revealed that REF6 positively regulates the general senescence process by directly up-regulating ETHYLENE INSENSITIVE 2 (EIN2), ORESARA1 (ORE1), NAC-LIKE, ACTIVATED BY AP3/PI (NAP), PYRUVATE ORTHOPHOSPHATE DIKINASE (PPDK), PHYTOALEXIN DEFICIENT 4 (PAD4), LIPOXYGENASE 1 (LOX1), NAC DOMAIN CONTAINING PROTEIN 3 (AtNAC3), and NAC TRANSCRIPTION FACTOR-LIKE 9 (NTL9), the key regulatory and functional genes predominantly involved in the regulation of developmental leaf senescence. Importantly, loss-of-function of REF6 increased H3K27me3 levels at all the target Senescence associated genes (SAGs). We therefore conclusively demonstrate that H3K27me3 methylation represents an epigenetic mechanism prohibiting the premature transcriptional activation of key developmentally up-regulated senescence regulatory as well as functional genes in Arabidopsis.

Semiconductor nanomaterial is a kind of important enhancement substrate in surface-enhanced Raman scattering (SERS), and the charge-transfer (CT) process contributes dominantly when they are used as the enhancement substrate for SERS. Doping has significant effect on the CT process of semiconductor nanomaterials. Yet till now, none attempts have been made to explore how doping affects the CT process between the semiconductor and probe molecules. For the first time, this paper investigates the effect of gallium (Ga) doping on the CT process between ZnO nanoparticles and 4-mercaptobenzoic acid (4-MBA) monolayer. In this paper, a series of Ga-doped ZnO nanoparticles (NPs) with various ratio of Ga and Zn are synthesized and their SERS performances are studied. The study shows that the doped Ga can cause the band gap shrinkage of ZnO NPs and then affect the CT resonance process form the valence band (VB) of ZnO NPs to the LUMO of 4-MBA molecules. The band gap of Ga-doped ZnO NPs is gradually narrowed with the increasing doping concentration, and a minimum value (3.16 eV) is reached with the Ga and Zn ratio of 3.8%, resulting in the maximum degree of CT. This work investigates the effects of doping induced band gap shrinkage on CT using SERS and provides a new insight on improving the SERS performance of semiconductor NPs.

An RMT-150B electrohydraulic servo testing system was used to perform uniaxial compression and uniaxial grading relaxation (creep) tests. The deformation, strength, and failure characteristics of the progressive failure process of coal samples under three loading modes were analyzed. The analysis results show that the prepeak stress-strain curve of the coal samples and the load relationships are not clear and that the whole compression process of coal still showed compression, elastic, yielding, and failure stages. The local stress drop characteristics during our relaxation creep grading tests showed no clear peak value and showed a yield curve with the shape of a conventional single plateau. The values of the mechanical parameters of axial compression were significantly higher than those obtained in the grade relaxation (creep) tests, which showed the mechanical parameters of coal samples with aging characteristics. In the relaxation (creep) tests, when the stress ratio was less than 70%, the relaxation (creep) characteristics of the sample were not clear. When the ratio of stress relaxation (creep) was more than 70% in the relaxation (creep) tests during displacement (stress) with a constant relaxation (creep) over the duration of the test, the evolution, development, and convergence of microcracks in the coal samples were observed. Relaxation (creep) stress was higher, failure duration was shorter, and the duration of failure was longer. For fully mechanized coal faces, increasing the support resistance and timely moving the support after coal cutting may prevent rib spalling accidents by reducing coal stress and exposure time in the front of the working face. Additionally, routine uniaxial compressive failures showed a simple form, having a clear tension-shear dual rupture surface. The staged relaxation creep failure testing of coal is more complex. The entire coal samples were divided into many thin-sheet debris via gradual collapse and shedding, and the number of cracks increased significantly, showing evident lateral expansion characteristics that are similar to the rib spalling characteristics in high coal mining working faces.

Third-party administration of dangerous chemicals is a management mode which improves the performance of enterprises, it helps enterprises reduce costs and increase competitiveness. However, ineffective third-party administration will also bring huge economic losses to enterprises. From the perspective of management science and technology, this paper studies the collaborative supervision method of third-party administrator for dangerous chemicals. Taking Jinan refinery as an example, this paper constructs a BSC-based KPI third-party administrator performance evaluation indicator system and introduces the risk matrix evaluation method and the Borda sequence value method to evaluate the key risks in the third-party administration. The results show that the lack of transparency and confidentiality, and the resistance from within the company are the main risks in the administration of dangerous chemicals of this company, so the risk prevention should be carried out from the three aspects of qualification review and signing of confidentiality contracts, personnel training of both parties and popularization and promotion of third-party administration. This study is of great significance for the theoretical research and practical application of the collaborative supervision method of third-party administrator for dangerous chemicals.

Market-based initiatives like agriculture value chain (AVC) are becoming progressively pervasive to support smallholder rural farmers and assist them in entering larger market interventions and providing a pathway of enhancing their socioeconomic well-being. Moreover, it may also foster staggering effects towards the post-era poverty alleviation in rural areas and possessed a significant theoretical and practical influence for modern agricultural development. The prime objective of the study is to explore the effects of smallholder farmers’ participation in the agricultural value chain for availing rural development and poverty alleviation. Specifically, we have crafted the assessment employing pre-production (improved fertilizers usage), in-production (modern preservation technology), and post-production (supply chain) participation and interventions of smallholder farmers. The empirical data has been collected from a micro survey dataset of 623 kiwifruit farmers from July to September in Shaanxi, China. We have employed propensity score matching (PSM), probit, and OLS models to explore the multidimensional poverty reduction impact and heterogeneity of farmers’ participation in the agricultural value chain. The results show that the total number of poor farmers who have experienced one-dimensional and two-dimensional poverty is relatively high (66.3%). We also find that farmers’ participation in agricultural value chain activities has a significant poverty reduction effect. The multidimensional poverty level of farmers using improved fertilizer, organizational acquisition, and using storage technology (compared with non-participating farmers) decreased by 30.1%, 46.5%, and 25.0%, respectively. The multidimensional poverty reduction degree of male farmers using improved fertilizer and participating in the organizational acquisition is greater than that of women. The multidimensional poverty reduction degree of female farmers using storage and fresh-keeping technology has a greater impact than the males using storage and improved storage technology. Government should widely promote the value chain in the form of pre-harvest, production, and post-harvest technology. The public–private partnership should also be strengthened for availing innovative technologies and infrastructure development.

The interface passivation of the HfO2/Si0.7Ge0.3 stack is systematically investigated based on low-temperature ozone oxidation and Si-cap methods. Compared with the Al2O3/Si0.7Ge0.3 stack, the dispersive feature and interface state density (Dit) of the HfO2/Si0.7Ge0.3 stack MOS (Metal-Oxide-Semiconductor) capacitor under ozone direct oxidation (pre-O sample) increases obviously. This is because the tiny amounts of GeOx in the formed interlayer (IL) oxide layer are more likely to diffuse into HfO2 and cause the HfO2/Si0.7Ge0.3 interface to deteriorate. Moreover, a post-HfO2-deposition (post-O) ozone indirect oxidation is proposed for the HfO2/Si0.7Ge0.3 stack; it is found that compared with pre-O sample, the Dit of the post-O sample decreases by about 50% due to less GeOx available in the IL layer. This is because the amount of oxygen atoms reaching the interface of HfO2/Si0.7Ge0.3 decreases and the thickness of IL in the post-O sample also decreases. To further reduce the Dit of the HfO2/Si0.7Ge0.3 interface, a Si-cap passivation with the optimal thickness of 1 nm is developed and an excellent HfO2/Si0.7Ge0.3 interface with Dit of 1.53 × 1011 eV−1cm−2 @ E−Ev = 0.36 eV is attained. After detailed analysis of the chemical structure of the HfO2/IL/Si-cap/Si0.7Ge0.3 using X-ray photoelectron spectroscopy (XPS), it is confirmed that the excellent HfO2/Si0.7Ge0.3 interface is realized by preventing the formation of Hf-silicate/Hf-germanate and Si oxide originating from the reaction between HfO2 and Si0.7Ge0.3 substrate.

The accurate recognition of the cloud phase has a great influence on the retrieval of the cloud top height. In order to improve the accuracy of obtaining the cloud top height with OCO-2, we proposed a cloud phase recognition algorithm based on the threshold of parameter α; α is defined as the reflectivity ratio of the region with weak continuous absorption of the oxygen A band to the region with weak continuous absorption of the CO2 1.6 µm band. The α under different solar zenith angles and different ground albedos was calculated. The results show the following: under the same surface albedo and solar zenith angle, α was large for ice clouds and small for water clouds. Under the same surface albedo, the greater the solar zenith angle, the smaller the α of the ice cloud, and the larger the α of the water cloud. Under the same solar zenith angle, the greater the surface albedo, the smaller the α; when the solar zenith angle was less than 70°, α can be used to effectively distinguish between the ice cloud and water cloud. This study used OCO-2 data of a single orbit over ocean to verify the feasibility of the algorithm through comparison with the CALIOP cloud phase product, which provided a basis for OCO-2 cloud top height estimation.

Hall-effect in semiconductors has wide applications for magnetic field sensing. Yet, a standard Hall sensor retains two problems: its linearity is affected by the non-uniformity of the current distribution; the sensitivity is bias-dependent, with linearity decreasing with increasing bias current. In order to improve the performance, we here propose a novel structure which realizes bias-free, photo-induced Hall sensors. The system consists of a semi-transparent metal Pt and a semiconductor Si or GaAs to form a Schottky contact. We systematically compared the photo-induced Schottky behaviors and Hall effects without net current flowing, depending on various magnetic fields, light intensities and wavelengths of Pt/GaAs and Pt/Si junctions. The electrical characteristics of the Schottky photo-diodes were fitted to obtain the barrier height as a function of light intensity. We show that the open-circuit Hall voltage of Pt/GaAs junction is orders of magnitude lower than that of Pt/Si, and the barrier height of GaAs is smaller. It should be attributed to the surface states in GaAs which block the carrier drifting. This work not only realizes the physical investigations of photo-induced Hall effects in Pt/GaAs and Pt/Si Schottky junctions, but also opens a new pathway for bias-free magnetic sensing with high linearity and sensitivity comparing to commercial Hall-sensors.

Antiretroviral therapy (ART) has dramatically suppressed human immunodeficiency virus (HIV) replication and become undetectable viremia. However, a small number of residual replication-competent HIV proviruses can still persist in a latent state even with lifelong ART, fueling viral rebound in HIV-infected patient subjects after treatment interruption. Therefore, the proviral reservoirs distributed in tissues in the body represent a major obstacle to a cure for HIV infection. Given unavailable HIV vaccine and a failure to eradicate HIV proviral reservoirs by current treatment, it is crucial to develop new therapeutic strategies to eliminate proviral reservoirs for ART-free HIV remission (functional cure), including a sterilizing cure (eradication of HIV reservoirs). This review highlights recent advances in the establishment and persistence of HIV proviral reservoirs, their detection, and potential eradication strategies.

The positioning, navigation, and timing (PNT) service of the Global Navigation Satellite System (GNSS) is developing in the direction of real time and high precision. However, there are some problems that restrict the development of real-time and high-precision PNT technology. Satellite orbit maneuvering is one of the factors that reduce the reliability of real-time navigation products, especially the high-frequency orbit maneuvering of geostationary earth orbit (GEO) and inclined geosynchronous orbit (IGSO) satellites. The BeiDou Navigation Satellite System (BDS) constellation is designed to contain GEO, IGSO, and medium earth orbit (MEO). These orbit maneuvers bring certain difficulties for data processing, especially for BeiDou satellites, such as decreased real-time service performance, which results in real-time navigation products including unusable maneuvered satellites. Additionally, the performance of real-time navigation products will decrease because the orbit maneuvers could not be known in advance, which diminishes the real-time PNT service performance of BDS for users. Common users cannot obtain maneuvering times and strategies owing to confidentiality, which can lead to a decline in the BDS real-time service performance. Thus, we propose a method to predict orbit maneuvers. BDS data from the broadcast ephemeris were analyzed to verify the availability of the proposed method. In addition, the results of real-time positioning were analyzed by using ultra-rapid orbit products, demonstrating that their reliability is improved by removing maneuvered satellites in advance. This is vital to improve the reliability of real-time navigation products and BDS service performance.

When detecting the presence of partial discharge (PD) activity in the insulation system in high-voltage equipment, the excitation voltages at variable frequency have been widely used instead of power-frequency (50/60 Hz) sinusoidal voltage in order to reduce the charging power. This work reviews the relevant research on PD activity at very low frequency (VLF) method, including sinusoidal or cosine-rectangular voltage shape, and damped AC (DAC) method. Based on the research history and development status, some major PD characteristics, such as PD inception voltage (PDIV), PD amplitude, PD charge, PD phase-resolved pattern, and several hot issues, such as surface charge decay and statistical time lag, have been discussed. Moreover, the advantages, disadvantages, and applied conditions of two reviewed methods has been summarized. Finally, the prospects have been made on the main development trends of this research field in the future.

We demonstrated an efficient system for multifocal structured illumination microscopy (MSIM) utilizing a spatial light modulator (SLM). Nine phase profiles of chessboard phase plates loaded on the SLM in sequence are used to generate nine multifocal arrays on the focal plane. Subsequently, nine raw multifocal images are acquired. Finally, by extracting the parameters of the illumination patterns from the raw images precisely, a final super-resolved image is reconstructed by performing the standard reconstruction procedure of structured illumination microscopy (SIM). Our MSIM system realized nearly a 1.5-fold enhancement in spatial resolution compared with wide-field (WF) microscopy. The feasibility of the present system is validated on experiments and the results show its great performances along with good compatibility.

Stacked SiGe/Si structures are widely used as the units for gate-all-around nanowire transistors (GAA NWTs) which are a promising candidate beyond fin field effective transistors (FinFETs) technologies in near future. These structures deal with a several challenges brought by the shrinking of device dimensions. The preparation of inner spacers is one of the most critical processes for GAA nano-scale transistors. This study focuses on two key processes: inner spacer film conformal deposition and accurate etching. The results show that low pressure chemical vapor deposition (LPCVD) silicon nitride has a good film filling effect; a precise and controllable silicon nitride inner spacer structure is prepared by using an inductively coupled plasma (ICP) tool and a new gas mixtures of CH2F2/CH4/O2/Ar. Silicon nitride inner spacer etch has a high etch selectivity ratio, exceeding 100:1 to Si and more than 30:1 to SiO2. High anisotropy with an excellent vertical/lateral etch ratio exceeding 80:1 is successfully demonstrated. It also provides a solution to the key process challenges of nano-transistors beyond 5 nm node.