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Objectives To investigate the frequency and prognostic role of deficient mismatch repair (dMMR) and RAS mutation in Chinese patients with colorectal carcinoma. Methods Clinical and pathological information from 813 patients were reviewed and recorded. Expression of mismatch repair proteins was tested by immunohistochemistry. Mutation analyses for RAS gene were performed by real-time polymerase chain reaction. Correlations of mismatch repair status and RAS mutation status with clinicopathological characteristics and disease survival were determined. Results The overall percentage of dMMR was 15.18% (121/797). The proportion of dMMR was higher in patients p p p p p p RAS mutation rate was 45.88%, including 42.56% (346/813) KRAS mutation and 3.69% (30/813) NRAS mutation (including three patients with mutations in both). KRAS mutation was significantly associated with mucinous production (p p p p KRAS mutation, however, dMMR tumors were less likely to have NRAS mutation. Moreover, dMMR, KRAS and NRAS mutation were not prognostic factors for stage I–III colorectal carcinoma. Conclusions This study confirms that the status of molecular markers involving mismatch repair status and RAS mutation reflects the specific clinicopathological characteristics of colorectal carcinoma.

To explore the effects of subunit influenza vaccines on mouse model of asthma.Six-week-old female BALB/c mice were randomly divided into PBS control group, asthma control group and subunit influenza vaccine group. Mice in the asthma control group and the subunit influenza vaccine group were sensitized on the 0, 7th, 14th day by intraperitoneal injection of 50 μg ovalbumin (OVA) emulsified in 2 mg aluminum hydroxide. The PBS control animals were given an equal volume of PBS. The mice in the subunit influenza vaccine group were immunized intramuscularly with 0.1 mL subunit influenza vaccines on the 21st day, and were boosted intranasally with 40 μL vaccines on the 28th day. Subsequently, the mice in the asthma control group and the subunit influenza vaccine group were exposed to OVA aerosol challenge for 3 consecutive days (the 42nd, 43rd, 44th day). Within 48 hours after the last challenge, all mice were sacrificed after the blood was obtained, and the bronchoalveolar lavage fluid (BALF) samples were collected for cell counting and classification. Lung tissues were prepared and HE staining and PAS staining were used to evaluate pulmonary inflammation and mucus production. IgE levels in sera and interleukine 4 (IL-4), IL-5, IL-13, interferon γ (IFN-γ) levels in BALF were measured by ELISA.Compared with the PBS control group, the pulmonary inflammation and mucus production significantly increased in the asthma control group and the subunit influenza vaccine group, and the levels of IL-4, IL-5, IL-13, IFN-γ in BALF and serum IgE were also significantly elevated. However, no significant difference was found between the subunit influenza vaccine group and the asthma control group.In mouse model of asthma, the use of subunit influenza vaccine does not exacerbate asthma symptoms, and is relatively safe.

The burning process of premixed hydrogen-oxygen gas in combustion light gas gun is quite dependent on the chemical reaction rate. This paper has established the single-zone mathematical model of hydrogen-oxygen combustion, and simulated the burning process of hydrogen-oxygen in combustion light gas gun with detailed chemical kinetic mechanisms. The computed results agree quite well with the experimental results. The effects of different chemical kinetic models on the burning process were analyzed and compared with each other, and the mole fraction changes of some chemistry species for different kinetic models during the burning process was studied respectively too. The results show that the induction time is earliest when using the single-step kinetic model, and latest when using the seven-step kinetic model, the formations of species H 2 O 2 and OH are particularly important to the combustion of hydrogen-oxygen, the chemical kinetic mechanism has an important impact on the hydrogen-oxygen combustion in combustion light gas gun.

To assess the feasibility of using saliva for Schistosomiasis japonica diagnosis.Schistosoma japonicum infected animal model was established. Pairs of saliva and serum samples from rabbits and chronic schistosomiasis patients were collected. Anti-schistosoma specific antibodies in saliva and serum were detected by indirect ELISA.The specificities of antibody detection of rabbit saliva and serum were 93% (28/30) and 97% (29/30), respectively, and the sensitivities of antibody detection of rabbit serum and saliva were 100% (24/24) and 88% (21/24), respectively. A significant correlation (r = 0.5307, P = 0.00380.05) existed between anti-SEA IgG levels in serum and saliva. As with those in serum, anti-SEA IgG levels in saliva could reflect the state of infection and treatment. The sensitivity of antibody detection was 91% (29/32) for patient saliva samples and 100% (32/32) for their sera. 8 samples were positive in 140 normal saliva samples (i.e. 6% false positive rate) and 6 samples were positive in 156 normal serum samples (4% false positive rate). There was a significant correlation (r = 0.4227, P = 0.0080.05) between specific antibodies in saliva and serum.The detection of specific antibodies in saliva can be used as a non-invasive immunodiagnosis method of Schistosomiasis japonica.

Multicolor emitting phosphors LiBaPO4:RE (RE = Eu2+, Tb3+, Sm3+) reported as interesting down-conversion luminescent materials in white light-emitting diodes (w-LEDs) have been prepared by a conventional solid-state reaction. The excitation and emission spectra indicate that these phosphors can be effectively excited by the near-UV light, and emit blue, green, and red light, respectively. The photoluminescence properties, decay times, and Commission Internationale de I’Eclairage (CIE) chromaticity indexes are determined for various concentrations of the activators Eu2+, Tb3+, and Sm3+ in LiBaPO4 host. Furthermore, the temperature-dependent luminescence of these phosphors was investigated. The emission intensities of Tb3+- and Sm3+-doped LiBaPO4 increased unexpectedly with increasing temperature. This interesting phenomenon is proposed to be the result of two ground states in the configurational coordination diagram. The present investigation suggests that LiBaPO4:RE (RE = Eu2+, Tb3+, Sm3+) were potential candidates for application in w-LEDs.