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870 results on '"Wu, Fu-Gen"'

211. MOESM1 of Constitutive hyperproduction of sorbicillinoids in Trichoderma reesei ZC121

Author

Chengcheng Li, Fengming Lin, Sun, Wei, Shaoxun Yuan, Zhihua Zhou, Wu, Fu-Gen, and Chen, Zhan

Abstract

Additional file 1: Fig. S1. Schematic illustration of the plasmid pXBthg-121121. hyg: homomycin resistance; LB, left border of binary vector; RB, right border of binary vector; Kan: kanamycin resistance; 121121-up: the 1500 bp upstream of gene 121121; 121121-down: the 1500 bp downstream of gene 121121. Fig. S2. The 1500-bp upstream and downstream regions of gene 121121. Fig. S3. The standard curve of sorbicillinoids. Fig. S4. The whole genome resequencing of strain ZC121 shows the unsuccessful deletion of gene 121121. Fig. S5. The color of the culture supernatant of T. reesei RUT-C30 (a) and ZC121 (b) grown on glucose. Fig. S6. Cellulolytic enzyme activities in the culture supernatant of T. reesei ZC121 and RUT-C30 grown on 2% cellulose, lactose, glucose, galactose and glycerol were assayed on day 5, including the activities of FPase (the filter paper activity) (a), pNPGase (the BGL activity) (b), pNPCase (the CBH activity) (c), CMCase (the CMC activity) (d) ahd pNPXase (the β-xylosidase activity) (e). The error bars indicate the standard deviations of three biological replicates. Table S1. Primers used in this study. Table S2. Comparative transcription levels of the sorbicillinoid gene cluster and its neighboring cellulase-related genes in T. reesei ZC121on cellulose and glucose.

Published
2018
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218. Strategies for visualizing inflammation.

Author

Zhang, Xinping, Liu, Xiaoyang, Guo, Yuxin, and Wu, Fu‐Gen

Abstract

As a basic innate immune response to the disordered tissue homeostasis, inflammation is related to the pathogenesis of multiple diseases, including bacterial infections, atherosclerosis, neurodegenerative diseases, and cancers. It is also a pivotal feature of some metabolic disorders such as diabetes and obesity. The visualization of in vivo inflammations can help us to comprehend the pathogenesis of these diseases and develop new solutions to diagnose them. Over the past few decades, a variety of strategies (eg, computed tomography, magnetic resonance imaging [MRI], and ultrasound [US] imaging) have been utilized for visualizing inflammations by imaging the structural changes of inflammatory tissues. Moreover, many recent studies have focused on some probes that can target or localize the inflammatory sites by specific binding to inflammation‐related molecules, being internalized by inflammatory cells, or becoming detectable only under inflammatory conditions. These probes can also be applied to visualize inflammations by MRI, positron emission tomography, single‐photon emission computed tomography, photoacoustic imaging, optical imaging (eg, fluorescence imaging, bioluminescence imaging, and chemiluminescence imaging), contrast‐enhanced US imaging, and the combined use of the abovementioned methods. This review not only summarizes the existing strategies for visualizing inflammations, but also discusses the limitations of the present strategies and the future directions on the development of new strategies for realizing the in vivo inflammation visualization. [ABSTRACT FROM AUTHOR]

Published
2021
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227. A Photo-triggered and photo-calibrated nitric oxide donor: Rational design, spectral characterizations, and biological applications

Author

He, Haihong, primary, Liu, Yuxin, additional, Zhou, Zhongneng, additional, Guo, Chunlei, additional, Wang, Hong-Yin, additional, Wang, Zhuang, additional, Wang, Xueli, additional, Zhang, Ziqian, additional, Wu, Fu-Gen, additional, Wang, Haolu, additional, Chen, Daijie, additional, Yang, Dahai, additional, Liang, Xiaowen, additional, Chen, Jinquan, additional, Zhou, Shengmin, additional, Liang, Xin, additional, Qian, Xuhong, additional, and Yang, Youjun, additional

Published
2018
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243. A Water-Soluble, Green-Light Triggered, and Photo-Calibrated Nitric Oxide Donor for Biological Applications

Author

He, Haihong, primary, Xia, Yang, additional, Qi, Yingxue, additional, Wang, Hong-Yin, additional, Wang, Zhuang, additional, Bao, Jianming, additional, Zhang, Ziqian, additional, Wu, Fu-Gen, additional, Wang, Haolu, additional, Chen, Daijie, additional, Yang, Dahai, additional, Liang, Xiaowen, additional, Chen, Jinquan, additional, Zhou, Shengmin, additional, Liang, Xin, additional, Qian, Xuhong, additional, and Yang, Youjun, additional

Published
2018
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244. Turning tumor cells into microvesicles as personalized cancer vaccines for cancer prevention and treatment.

Author

Guo, Yuxin, Zhang, Xinping, Wang, Shao-Zhe, Xu, Yin, Jia, Hao-Ran, Zhu, Ya-Xuan, Wu, Shun-Yu, Zhang, Xiaodong, Feng, Hui-Heng, Gao, Ge, Li, Zi-Heng, and Wu, Fu-Gen

Subjects
CANCER vaccines, CANCER prevention, EXTRACELLULAR vesicles, CANCER treatment, FREEZE-thaw cycles, LYMPH nodes
Abstract

Autologous cancer cell-derived vaccines can arouse potent and patient-specific immunostimulation for cancer immune therapy. Here, we report a novel and simple preparation strategy of cancer cell-derived vaccines. A series of nanoinducers (NIs) are fabricated to effectively transform different kinds of cells into micrometer-sized vesicles (termed MVs) with a very high cell-to-MV conversion rate (e.g., ∼35%, based on protein content). We surprisingly find that these cancer cells-derived MVs can be used as cancer vaccines to arouse efficient anticancer immune responses. Compared to the commonly used cell debris (e.g., the sonication- and freeze-thaw-obtained cell debris), the MVs exhibit a much stronger immunostimulation effect, which is attributed to the higher proportion of immune-related proteins in MVs. After subcutaneous administration, the MVs can massively target lymph nodes and elicit systemic immunostimulation. Preimmunization with MVs together with an adjuvant can protect the mice from both subcutaneous and intravenous 4T1 tumor challenges, and the MVs plus adjuvant also show satisfactory anticancer outcomes in tumor-bearing mice. Furthermore, the MVs can be massively induced and easily purified, which may facilitate their clinical translation. It is believed that such a cell-derived cancer vaccine may find practical applications for personalized cancer prevention and treatment. [Display omitted] • A novel preparation strategy of autologous cancer cell-derived vaccines is reported. • A series of nanoinducers are fabricated to convert cancer cells into microvesicles. • The microvesicles have a stronger immunostimulation effect than cell-derived debris. • The microvesicle plus an adjuvant shows a satisfactory anticancer outcome. [ABSTRACT FROM AUTHOR]

Published
2024
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245. Back Cover: A red blood cell‐derived bionic microrobot capable of hierarchically adapting to five critical stages in systemic drug delivery (EXP2 2/2024).

Author

Zhu, Ya‐Xuan, Jia, Hao‐Ran, Jiang, Yao‐Wen, Guo, Yuxin, Duan, Qiu‐Yi, Xu, Ke‐Fei, Shan, Bai‐Hui, Liu, Xiaoyang, Chen, Xiaokai, and Wu, Fu‐Gen

Subjects
BIONICS, DRUGS, ANTINEOPLASTIC agents
Abstract

This article discusses the development of a red blood cell-derived microrobot that can be used for systemic drug delivery. The microrobot is designed to overcome biological barriers and has the ability to circulate in blood vessels, target tumors using magnetic guidance, release therapeutic cargoes when triggered by light, and facilitate drug penetration in tumors by damaging the extracellular matrix. The researchers believe that this technology has the potential to improve anticancer therapeutic efficacy. The article is authored by a team of researchers and was published in the journal Exploration. [Extracted from the article]

Published
2024
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