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W doświadczeniach infekcyjnych w warunkach polowych w Radzikowie i Poznaniu/Cerekwicy badano odporność na fuzariozę kłosów 224 genotypów oraz 10 odmian/linii wzorcowych pszenicy ozimej. Genotypy te pochodziły z programów hodowlanych pszenicy ozimej oraz z kolekcji utworzonej w wyniku badań nad odpornością na fuzariozę kłosów w latach 2008–2013. Kłosy pszenicy inoklulowane były izolatmi Fusarium culmorum. Oceniano porażenie kłosa oraz stopień uszkodzenia ziarniaków. W ziarnie wybranych 61 genotypów analizowano zawartość ergosterolu, trichotecenów z grupy B oraz zearalenonu. Genotypy pszenicy były istotnie zróżnicowane pod względem odporności na fuzariozę kłosów. Średni indeks fuzariozy wyniósł 23,7%. Stwierdzono istotny wpływ wysokości roślin na wartość indeksu fuzariozy kłosów (r = -0,528). Badane genotypy wykazały zróżnicowaną odporność na uszkodzenie ziarniaków przez Fusarium (średnio 48,4%) oraz zróżnicowaną zawartość ergosterolu w ziarnie (średnio 30,9 mg·kg-1). Nasilenie fuzariozy kłosów istotnie korelowało z uszkodzeniem ziarniaków pszenicy (r = 0,768). Genotypy pszenicy wykazały zróżnicowaną odporność na akumulację toksyn fuzaryjnych. Średnia zwartość deoksyniwalenol (+pochodne acetylowe) wyniosła 20,515 mg·kg-1, natomiast zearalenonu 932 g·kg-1. Stopień porażenia kłosów, odporność typu II oraz uszkodzenie ziarniaków korelowały istotnie z zawartością ergosterolu, deoksyniwalenolu i zearalenonu w ziarnie. Zidentyfikowano genotypy łączące odporności różnych typów na fuzariozę kłosów.

Acta of Bioengineering and Biomechanics; 03/2018; ISSN 1509-409X

The article regards the cultural role of Bydgoszcz hydrography from a historical perspective. In old times, the layout of the hydrographic system of the Bydgoszcz area was conducive to long-distance travels of hunting tribes in the Upper Paleolithic. Along with stabilization of the Holocene climate, it attracted representatives of hunting and fishing Mesolithic cultures, followed by settled Neolithic communities. During the Bronze Age and Early Iron Age, settlement gradually developed in the proximity of smaller water bodies feeding the Brda and Vistula rivers within their Bydgoszcz courses. During the Roman period, the Brda served as a trail connecting the regions of Pomerania and Wielkopolska. Starting from the Middle Ages, local people had been using all advantages of the Bydgoszcz hydrographic network. It can be confirmed by discoveries of dugout canoe and Vistulan punt wrecks in downtown area. Close relations between the Brda and Vistula rivers and the Old-Polish culture are best described by a poem dedicated to “ship launches on the Vistula” called Flis, which is the traditional name of the water body and district situated in the western part of Bydgoszcz., Kronika Bydgoska, Tom 38 (2017): Kronika Bydgoska

We investigate a SAT-based bounded model checking (BMC) method for MTL (metric temporal logic) that is interpreted over linear discrete infinite time models generated by discrete timed automata. In particular, we translate the existential model checking problem for MTL to the existential model checking problem for a variant of linear temporal logic (called HLTL), and we provide a SAT-based BMC technique for HLTL. We show how to implement the BMC technique for HLTL and discrete timed automata, and as a case study we apply the technique in the analysis of GTPP, a Generic Timed Pipeline Paradigm modelled by a network of discrete timed automata.

Simple synthetic procedure, reaction of Os[P(C 6 H 5 ) 3 ] 2 (CO) 2 Cl 2 with 2-phenylpyridine (or other similar N∩CH chelating ligands) in boiling 2-(2-ethoxyethoxy)ethanol solution leads to Os[P(C 6 H 5 ) 3 ] 2 (CO)(N∩C − )Cl complexes. Structures of the obtained species have been elucidated by means of 31 P NMR and FT-IR techniques as well as elemental analysis data. The UV–vis absorption and emission properties of the newly synthesized complexes reveal the MLCT character of their lowest singlet and triplet excited states.

SummaryThe current study aimed to investigate differential expression of inhibin βA (INHβA) and inhibin βB (INHβB) in porcine oocytes before or after in vitro maturation (IVM) isolated from follicles of various sizes. Porcine oocytes isolated from large, medium and small follicles (40 from each) were used to study the INHβA and INHβB protein expression pattern using western blot analysis before or after 44 h of oocyte IVM. An increased expression of INHβA was found in oocytes collected from large and medium follicles compared with small follicles before or after IVM (P < 0.001, P < 0.05, respectively). Similarly, higher INHβB levels were observed in oocytes recovered from large follicles compared with small (P < 0.01). As INHβA and INHβB are expressed in both porcine follicular somatic cells and oocytes, it can be assumed that these transforming growth factor beta (TGFβ) superfamily factors are involved in the regulation of molecular bi-directional pathways during follicle and oocyte development, and can be recognized as markers of follicle and oocyte maturation. Moreover, the current study clearly demonstrated that inhibin expression is substantially associated with porcine follicle growth and development.

Celem badań było porównanie wyników oceny mrozoodporności i zimotrwałości 426 rodów i odmian pszenicy ozimej badanych w latach 2010–2012. Stwierdzono wysoce istotną korelację pomiędzy wynikami ocen obu metod wynoszącą w 2010 roku, r = 0,683, w 2011 roku, r=0,561, a w 2012 roku r=0,614. Ważnymi czynnikami w ocenie mrozoodporności i zimotrwałości okazały sie warunki środowiska, które należy uwzględnić w trakcie prowadzenia badań oraz podczas analizy otrzymanych wyników.

The TGFB superfamily genes are involved in several important cell functions, including proliferation and differentiation, and the role of the expression of these genes in growth and development of theca and granulosa cells is well recognised. However, the dependence between the stage of oocyte maturation or follicular size and the expression of these genes in pigs is still not entirely known. This study was aimed at investigating the expression pattern of GDF9, TGFB1, TGFB2 and TGFB3 in porcine oocytes before and after in vitro maturation (IVM) as well as in oocytes collected from follicles of different sizes. RQ-PCR was performed to analyse the expression of GDF9, TGFB1, TGFB2 and TGFB3 in oocytes before and after IVM (oocytes cultured for 44 h in TCM-199), isolated from large (> 5 mm), medium (3–5 mm) and small (< 3 mm) follicles collected from ovaries of 28 puberal crossbred Landrace gilts after slaughter. We found an increased expression of both TGFB1 and TGFB2 in oocytes before IVM collected from large as compared to medium and small follicles (P < 0.05, P < 0.001, P < 0.01, P < 0.05, respectively). In these groups of oocytes we did not observe differences in GDF9 and TGFB3 mRNA levels. However, after IVM, GDF9 protein distribution in oocytes was significantly higher in large and medium follicles as compared to small ones (P < 0.01, P < 0.001, respectively). Moreover, an increased TGFB1, TGFB2 and TGFB3 proteins pattern was observed in oocytes of large compared to small follicles. The highest GDF9 and TGFB1 mRNA levels were found in oocytes after IVM compared to those before IVM. Based on our study we can suppose that the distribution pattern of TGFB superfamily genes is associated with the stage of maturation of porcine oocytes and the follicle size. Furthermore, GDF9 and TGFB1 may serve as molecular markers of the develop-mental potential of porcine oocytes. The confocal microscopic observation revealed that TGFB1 and TGFB3 were translocated between the zona and the cytoplasm of oocytes, depending on the stage of maturation and follicle size.

The CC (cumulus cell) proliferation index in relation to the expression and distribution of Cdk4 and Cx43 proteins, which are crucial factors for oocyte maturation, was investigated. Cumulus-oocyte complexes (COCs) were recovered from pubertal crossbred Landrace gilts and treated with collagenase, and separated CCs were cultured in standard TCM199 medium for 44 h. At each step of in vitro cultivation (IVC) of CCs (0, 12, 24 and 44 h), a normalized proliferation index was assessed. Cdk4 and Cx43 protein expression and the CC-specific cellular distribution were analyzed by confocal microscopic observation. The normalized proliferation index (number of cells attached, measured by impedance) was increased in the first 12 h of IVC (P

In the paper we propose a translation of the existential model checking problem for timed automata and properties expressible in MITL to the existential model checking problem for HLTL. Such a translation, for example, allows to adopt LTL bounded model checking method for verification of the MITL properties.

This paper makes two contributions to the verification of multi-agent systems modelled by interleaved interpreted systems. Firstly, the paper presents theoretical underpinnings of the SAT-based bounded model checking (BMC) approach for LTL extended with the epistemic component (LTLK) over interleaved interpreted systems. Secondly, the BMC method has been implemented and tested on several benchmarks for MAS. The preliminary experimental results reveal advantages and disadvantages of our SAT-based BMC for LTLK and show that the method has a significant potential.

Abstract Gap junction connections are formed by proteins which play an important role in oocyte developmental competency but there is little information on the relationship between follicle size and the expression of genes encoding these proteins. The aim of this study was to investigate the potential association between follicle size and the levels of Cdk4 and Cx43 proteins using western blot analysis and confocal microscopic observations. Cumulus-oocyte complexes (COCs) were collected from puberal gilts (n = 20) of large (>5 mm), medium (3-5 mm), and small (

We hypothesized that oocyte morphology may be associated with the accumulation of specific mRNAs encoding proteins responsible for the gamete fertilization ability. Therefore, the aim of the study was to evaluate the transcript levels of porcine zona pellucida (pZP1, pZP2, pZP3 and pZP4) glycoproteins in oocytes classified by a four-grade morphological scale (I-IV) accounting for either a homogeneous cytoplasm and a complete cumulus oophorus (grade I) or a heterogenous cytoplasm and decreased number of cumulus layers in the other grades (II, III and IV). We observed a significant increase of all investigated pZP glycoprotein mRNAs in grade I oocytes as compared to other grades (p

The model checking tools Uppaal and VerICS accept a description of a network of Timed Automata with Discrete Data (TADDs) as input. Thus, to verify a concurrent programwritten in Java by means of these tools, first a TADD model of the program must be build. Therefore, we have developed the J2TADD tool that translates a Java program to a network of TADDs; the paper presents this tool. The J2TADD tool works in two stages. The first one consists in translation of a Java code to an internal assembly language (IAL). Then, the resulting assembly code is translated to a network of TADDs. We exemplify the use of the translator by means of the following well-known concurrency examples written in Java: race condition problem, dining philosophers problem, single sleeping barber problem and readers and writers problem.

This paper aims to present the involvement of Cracovian musicologists and musicians in a series of lectures organised between 1904 and 1939 for the common public in and outside Kraków by the Jagiellonian University. The questions addressed in this paper have been divided into four sections: 1. The Outline of Public University Lectures in and outside Kraków; 2. The first talks by the music journalist and pianist Franciszek Bylicki; 3. Regular lectures on history of music run by musicologists and musicians (Zdzisław Jachimecki, Józef Reiss, Stanisław Bursa, Stanisław Golachowski); 4. Popularity of music lectures and their topics. The tables show detailed lists of locations, dates and subjects discussed during the talks on the history of music, as well as representative numbers of their audiences, all supported with source references. The type of lecturers’ involvement and topics of their talks have been presented in the context of their academic, writing and artistic achievements.

We evaluate the downstream performance of our novel interoperator fixed-mobile network sharing, in which operators exchange data in their access networks. We propose a performance evaluation algorithm, and report credible performance evaluation results obtained for 204600 randomly-generated passive optical networks. We show that with the proposed sharing, operators can increase their access network performance even twofold with software-defined upgrades, and with no or minimal new hardware required., Comment: submitted to a conference

The luminescence properties of the heteroleptic fac-Re(CO)3(+) complexes with α-diimine N∩N ligands, neutral [Re(CO)3(N∩N)Cl] and cationic [Re(CO)3(N∩N)(CH3CN)](+) species, have been studied in acetonitrile solutions at room temperature. The investigated complexes exhibit the metal to ligand charge-transfer (MLCT) phosphorescence with the emission characteristics strongly affected by the nature of coordinated α-diimine N∩N ligands. The observed trends can be quantitatively described by invoking the electronic interactions between (3)*LC and (3)*MLCT states as well as the spin-orbit interactions between (3)*MLCT and (1)*MLCT states, respectively. All quantities necessary for the description can be straightforwardly accounted from analysis of the radiative (1)*MLCT ← S0 and (3)*MLCT → S0 charge transfer processes. It is also demonstrated that the radiative kr and non-radiative knr decay rate constants of the excited (3)*MLCT states can be interpreted within the same set of parameters. As expected from the Mulliken-Hush formalism the both processes are strictly related that allows prediction of the non-radiative knr rate constants using the parameters available from analysis of the radiative (1)*MLCT ← S0 and (3)*MLCT → S0 charge transfer processes.

Background The utilization of the radial access (RA) for percutaneous coronary intervention (PCI) has gradually increased. Recent studies suggest an advantage over femoral access (FA) in high-risk patients presenting with ST-segment elevation myocardial infarction (STEMI). The aim was to evaluate bleeding complications and periprocedural outcomes with safety and efficacy of RA compared with FA for PCI with stent implantation in “real-world” patients with STEMI presentation from Polish National Registry. Methods The study group consisted of 22,812 consecutive patients with STEMI treated with PCI and stent implantation between January 2014 and June 2015 in 151 tertiary invasive cardiology centers in Poland. All data were stored in electronic database of National PCI Registry (ORPKI). Patients treated with RA and FA were compared using a propensity score analysis to best match between groups. The analysis was done in the “as-treated” manner. Results Femoral access and RA were used in 9,334 (40.9%) and 13,478 (59.1%) patients, respectively. After propensity score matching no differences in baseline characteristic between 6,542 matched pairs was found. Significantly higher total amount of contrast (191.8 ±8 ml vs. 174.8 ±68.8 ml, p = 0.0001) and lower radiation doses were used in FA (1279.5 ±1346.3 mGy vs. 1182.6 ±887 mGy, p = 0.02). More bleeding complications at puncture site after both angiography (0.17% vs. 0.02%; p = 0.004) and PCI (0.23% vs. 0.09%, p = 0.049) were reported in FA group. Periprocedural death (1.94% vs. 0.93%, p = 0.0001) and periprocedural cardiac arrest (1.44% vs. 0.96%, p = 0.01) occurred significantly more often after PCI performed with FA. Conclusions Radial access was associated with lower incidence of periprocedural death in STEMI patients as well as a significant reduction of bleeding complications at access site., Background Little is known on the impact of psoriasis on the outcome of percutaneous cardiovascular procedures. The aim of this analysis was to identify the incidence of active psoriasis, baseline characteristic, and periprocedural to in-hospital outcomes in this subgroup of patients. Methods The Polish National PCI database (ORPKI) is a mandatory registry for all percutaneous cardiology procedures performed in Poland since 2004. All consecutive patients who had coronary angiography or coronary angiography with immediate PCI in 155 interventional cardiology centers in Poland in 2014 for either stable angina or acute coronary syndrome were included. Patients with active psoriasis on admission were identified. Allergic origin of the periprocedural complication was defined if a typical reaction from rash to anaphylaxis was diagnosed. Results There were 206,335 patients with complete records in the database. Active psoriasis was diagnosed in 830 of them (0.4%). Patients with psoriasis were younger (63.1 ±10.6 years vs. 66.2 ±10.9 years, p < 0.01), significantly more often with diabetes mellitus, arterial hypertension and chronic kidney disease. The periprocedural mortality for patients with psoriasis (0.77%) was similar to those without (0.55%; p = 0.6). Interestingly, allergic reaction occurrence during angiography was 0.77% in patients with psoriasis vs. 0.07% in patients without psoriasis (p < 0.001). In multivariate regression analysis, the diagnosis of active psoriasis was an independent predictor of the occurrence of an allergic reaction during coronary angiography/PCI with OR = 8.3 and 95% CI: 1.98–34.93 (p = 0.004). Conclusions Psoriasis is associated with a different patient baseline profile and an increased risk for allergic reaction occurrence during the procedure. Best to our knowledge, this is the first report of such relationship and requires further study., Background Neovascularization in restenotic tissue may play a key role in neointimal proliferation and progression of neoatherosclerosis. There are still insufficient in vivo data regarding in-stent neovascularization. Methods We evaluated 48 stent segments from 24 stents implanted in porcine model of coronary artery injury. Optical coherence tomography (OCT) was performed 90 days post implantation. All measurements and qualitative analysis were performed by two independent investigators. Microvessels were defined as well delineated low backscattering structures with diameter less than 200 microns that show a trajectory within the vessel. There were two groups: with (1) or without (2) occurrence of neovascularization. Optical coherence tomography findings were compared between both groups. Results There were no statistically significant differences between the two groups, but lesions with microvessels had a larger average stent area (8.83 ±1.39 vs. 8.27 ±1.36, p = NS) and larger average neointimal area (2.62 ±1.46 vs. 1.95 ±0.87, p = NS) than those without microvessels. Minimal lumen cross-sectional area (CSA) were almost identical in both groups (2.56 ±0.47 vs. 2.57 ±0.33, p = NS). Conclusions Occurrence of neovascularization might be associated with the extent of neointimal area, but further studies are required to estimate factors associated with strut neovascularization., Background The previous study using intravascular imaging presented that the part of the treated atheroma is squeezed out of the vessel by the stent and its remnants may embolize. The present study aimed to characterize the plaque redistribution with relation to its composition. Methods The combined near-infrared spectroscopy (NIRS) and intravascular ultrasound (IVUS) imaging analyzed the plaque distribution after the stent implantation in vivo in patients with a stable coronary artery disease (CAD) (n = 33) and acute coronary syndromes (n = 16). Near-infrared spectroscopy detected lipids in the lesion and IVUS presented the redistribution of the plaque. The maximal lipid core burden index in a 4 mm long segment (LCBI4mm) was estimated by NIRS before and after the stent implantation, and IVUS estimated minimal lumen area (MLA), plaque burden (PB) and plaque volume pre and post implantation. Results NIRS-IVUS imaging was performed before and after the implantation of 50 stents in 49 patients. The median plaque volume pre and post implantation was 127.45 (74.5, 186.8) mm2 vs. 99.35 (67.5, 171.5) mm2 (p < 0.001) with a median difference 19.25 (3.9, 72) mm2. The median LCBI4mm pre and post stent was 351 (157, 589) vs. 77 (3, 231) (p < 0.001) in the treated lesions, and 34 (0, 207) vs. 0 (0, 45) (p < 0.04) in segment 4 mm long proximally to the stent. Plaque burden in 4 mm proximally to the implanted stent increased from 46.23 ±11.79 to 50.36 ±9.93 post stenting (p = 0.02). The difference in the plaque volume pre and post stenting correlated with the value of LCBI4max of the treated lesion (r = 0.457, p = 0.001). The median difference in the plaque volume was higher for stented lesions with LCBI 4mm > 265 (8.2 (1.4, 19.3) vs. 24.3 (12.1, 45.0), p = 0.012) (Figure 1). Figure 1 Case example of the combined near-infrared spectroscopy and intravascular ultrasound imaging Conclusions Lipid-rich lesions characterized greater release of the atheroma remnants to the coronary artery lumen as compared to non-lipid rich lesions. The stent implantation causes plaque shift proximally to the implanted stent., Background Adenosine is commonly used to induce maximal hyperemia to evaluate the hemodynamic significance of coronary stenoses with measuring fractional flow reserve (FFR). So far, limited number of studies validated the intracoronary application of adenosine against standard intravenous infusion. Thus, we aimed to assess agreement and reproducibility of FFR measurements during intracoronary vs. intravenous administration of adenosine. Methods Consecutive patients with angiographically borderline coronary lesions (40–90%) diameter stenosis in visual assessment), who were scheduled for FFR were enrolled. Patients received intravenous adenosine infusion via a 6-F femoral venous sheath. Adenosine was administered at 140 at 280 μg/kg/min. Intracoronary adenosine bolus injection was performed with 100 μg, 200 μg, 400 μg and 600 μg. Results Femoral vein adenosine at 140 and 280 μg/ kg/min as well as intracoronary boluses recordings from 81 vessels in 32 patients were suitable for blinded analysis. The median FFR measured using adenosine administered via femoral routes at 140 μg/kg/min was 0.82 (IQR: 0.76–0.89) and at 280 μg/kg/min – 0.82 (IQR: 0.75–0.88), p = 0.001. However, a strong correlation between them was found r = 0.99. Median FFR values for 100 μg, 200 μg, 400 μg and 600 μg bolus were: 0.84, 0.83, 0.83 and 0.83, respectively. We found numerically higher values of FFR assessed with boluses comparing to infusion with the mean difference of FFR from 0.01 (bolus 600 μg) to 0.02 (bolus 100 μg). We did not find any differences between 400 and 600 μg bolus (p = 0.21). Differences between boluses and infusion are shown in details on Figure 1. Figure 1 Mean values of fractional flow reserve assessed with adenosine i.c. boluses and i.v. infusion Conclusions Values of FFR achieved with adenosine infusion via femoral vein and with intracoronary boluses are consistent. However, there seems to be a grey zone for FFR assessed with boluses which may indicate, in selected cases, the use of femoral vein infusion to confirm the results., Background Rotational atherectomy (RA) is often used as a bailout technique after unsuccessful percutaneous coronary intervention (PCI). This study was created to evaluate outcomes of patients undergoing RA depending on reason of previous PCI failure. Methods We retrospectively evaluated data of 210 consecutive patients undergoing RA in our tertiary center. From our analysis we excluded patients undergoing RA without previous PCI attempt (n = 42), those referred from other centers without description of PCI failure cause (n = 10) or lacking complete clinical data (n = 6). Remaining patients (n = 152) were divided into two groups: those with uncrossable (32%) or undilatable lesions (68%). Follow- up data concerning death from any cause was acquired from the Polish National Health Found. Results Patients with uncrossable lesions were older (72 vs. 69 years; p = 0.03) and more often suffered from stroke or transient ischemic attack in anamnesis (20% vs. 8%, p = 0.04). The groups did not differ in any other clinical characteristics. We also did not show any differences in procedure characteristics, success and complications rates or in-hospital adverse events. Patients with uncrossable lesions had higher all-cause mortality after 1 year (18% vs. 8%; p = 0.05), 2 years (24% vs. 10%; p = 0.02) and 3 years (29% vs. 14%; p = 0.03). Multivariate Cox regression model showed uncrossable coronary lesions to be an independent predicting factor of all-cause mortality 2 years after procedure (HR = 2.8; 95% CI: 1.13–7.15; p = 0.03). Conclusions Patients undergoing RA after failed PCI due to uncrossable lesions have poorer long term prognosis. Further studies evaluating causes of higher mortality in this population are needed., Background This study was created to assess the frequency and influence of severe coronary artery calcification (CAC) in target lesions on adverse cardiac events in patients undergoing percutaneous coronary interventions (PCI) due to acute myocardial infarction. Methods We prospectively evaluated data of 206 consecutive patients who underwent PCI due to acute myocardial infarction. The pre-specified angiographic definition of severe calcifications was the presence of radiopaque densities visible without heart motion and affecting both sides of the treated lesion. Follow-up data concerning primary end points was acquired from the Polish National Health Found. Results Severe CAC in target lesions were present in 35 (17%) patients. These patients were older (71 vs. 65 years, p = 0.02), were more often diagnosed with non-ST elevation myocardial infarction (NSTEMI, 77% vs. 58%, p = 0.03) and had higher levels of NT-proBNP at admission (2006 pg/ml vs. 745 pg/ml, p = 0.01). Full revascularization during index procedure was achieved less often in patients with severe CAC (14% vs. 41%, p = 0.003). During 30 months of follow-up patients with severe CAC more often suffered from another ACS (37% vs. 13%, p = 0.0005) and had higher all-cause mortality (31% vs. 16%, p = 0.04). Multivariate Cox regression model showed severe CAC in target lesions to be the only independent predicting factor of another ACS in studied population (hazard ratio = 2.78; 95% confidence interval: 1.39–5.56; p = 0.004). Conclusions In everyday practice, almost every sixth patient with myocardial infarction has severe CAC in target lesions. This group has poorer prognosis then the rest of the population. Presence of severe CAC in target lesions independently predicts recurrent ACS., Background Rotational atherectomy (RA) is s method of treatment of highly calcified lesions that cannot be treated with traditional percutaneous coronary intervention (PCI). However, RA in patients with acute coronary syndromes (ACS) remains controversial and may be contraindicated. Methods The study included 207 consecutive patients, who underwent RA. A retrospective analysis of data in subjects presenting with ACS (n = 42) or treated electively (n = 165) was done. Results Patients presenting with ACS were older, had higher systolic blood pressure and heart rate on admission, they were less likely to undergo PCI before and had lower total left ventricular ejection fraction (LVEF). Estimated risk and lesion complexity were higher in the ACS population. We found no differences in procedural aspects. We noted higher rates of contrast-induced nephropathy and bleeding in the ACS group. During in-hospital observation 2 cases of death occurred in the ACS group, but we found no difference in total mortality, ACS, decompensated HF or stroke rates in 1-year follow-up. Analysis of Cox hazard risk model revealed PCI failure due to uncrossable lesion to be risk factor of total 1-year mortality in the whole studied group; increasing LVEF improved the prognosis. Detailed data are presented in the Table I (see page 396). Table I General characteristics, risk factors and comorbidities Parameter All patients (n = 207) Elective (n = 165) ACS (n = 42) P-value Age, mean ± SD [years] 71 ±2 70 ±1 75 ±4 < 0.001 Previous ACS, n (%) 130 (63) 99 (60) 31 (74) NS Previous PCI, n (%) 152 (74) 128 (78) 24 (57) < 0.01 Previous CABG, n (%) 31 (15) 26 (15) 6 (14) NS Heart failure, n (%) 70 (34) 50 (30) 20 (48) < 0.05 LVEF, mean ± SD [%] 50 ±11 51 ±11 47 ±13 < 0.05 LVEF < 35%, n (%) 34 (17) 22 (14) 12 (29) < 0.05 Procedural data EuroScore II mortality rate, median (IQR) 2.4 (1.4-4.9) 2.1 (1.3-3.8) 5.15 (2.6-9.3) < 0.0001 Syntax Score, median (IQR) 17 (11-24) 17 (11-24) 21.5 (15-29) < 0.05 CABG inoperable, n (%) 116 (56) 86 (52) 30 (41) < 0.05 Attempted PCI failure, n (%): Nondilatable lesion 105 (51) 86 (52) 19 (45) NS Uncrossable lesion 48 (23) 38 (23) 10 (24) NS Unknown 11 (5) 9 (6) 2 (5) NS Procedure success, n (%) 184 (89) 147 (89) 37 (88) NS Ad hoc procedure, n (%) 6 (4) 7 (4) 2 (5) NS Radial access, n (%) 125 (60) 99 (60) 26 (62) NS GP IIb/IIIa inhibitor, n (%) 5 (2) 5 (3) 0 NS IABP n (%) 4 (2) 2 (1) 2 (5) NS Outcomes CIN, n (%) 6 (3) 2 (1) 4 (10) < 0.05 ARC major bleeding, n (%) 14 (7) 8 (5) 6 (14) < 0.05 In-hospital mortality, n (%) 2 (1) 0 2 (5) < 0.05 Inhospital stroke/TIA, n (%) 1 (0.5) 1 (1) 0 NS Inhospital MI, n (%) 28 (14) 22 (13) 6 (14) NS Inhospital TVR, n (%) 1 (0.5) 0 1 (2) NS Total 1-year mortality, n (%) 20 (10) 14 (8) 6 (14) NS 1-year MI, n (%) 20 (8) 15 (9) 5 (12) NS 1-year ADHF n (%) 12 (6) 9 (5) 3 (7) NS 1-year stroke/TIA, n (%) 2 (1) 2 (1) 0 NS Predictors of 1-year mortality (n = 207) Parameter HR 95% CI P-value LVEF (%) 0.95 0.91-0.99 < 0.05 PCI failure: uncrossable lesion 4.00 1.57-10.20 < 0.01 Conclusions One-fifth of RA candidates present with ACS. Despite higher periprocedural mortality rate in ACS group, we found no significant difference in 1-year outcomes in comparison with elective patients. Rotational atherectomy in patients with ACS in experienced centre should be considered in that particular setting if no other options of treatment exist., Background Some patients receiving dual antiplatelet therapy after percutaneous coronary interventions (PCI) with drug-eluting stent (DES) implantation require surgery to be performed during dual antiplatelet therapy (DAPT) administration period. Aim of our research was to assess the risk of premature discontinuation of DAPT after PCI with second generation DES implantation in patients undergoing major vascular surgeries. Methods Our study group consist of 88 patients who underwent PCI with second generation DES implantation between 2010 and 2014 and underwent major vascular surgery up to 12 months after PCI, requiring discontinuation of DAPT in perioperative period. We assessed the frequency of death, myocardial infarction (MI), major bleeding episodes and other adverse events in short and long-term observation. Results Patient characteristics are shown in Table I (see page 397). In perioperative period, we reported no MI, death, or major bleeding. During the follow up period of mean 2.71 (0.16–5.75) years, we recorded 14 (15.9%) deaths: 7 (7.9%) heart related, 4 (4.5%) cancer related, 3 (3.4%) others. In a multivariate analysis, the only independent factor of mortality was left ventricular ejection fraction (LVEF; OR = 0.93; 95 CI: 0.87–0.99; p = 0.02). Time between procedures was not a predictor of mortality and MI. Table I Patient characteristics Variables Values Study group 88 Mean age [years] 69 ±8.3 Sex, male 58 (66%) Mean time between procedures [days] 94.4 ±84.4 Diabetes 31 (35.2%) Mean EF 49.4% Arterial hypertension 73 (83.0%) Atrial fibrillation 8 (9.1%) Recent MI 32 (36.4%) PCI in history 31 (35.2%) CABG in history 9 (10.2%) PAD 67 (76.1%) Stenotic carotid arteries 23 (26.1%) Conclusions In patients with peripheral atherosclerosis requiring major vascular surgery after PCI with second generation DES implantation, early discontinuation of DAPT – on average 90 days after PCI is not associated with increased risk of death, MI, or major bleeding., Background This study aimed to evaluate gender-related differences in short- and long-term outcomes of patients undergoing balloon aortic valvuloplasty (BAV) for severe aortic stenosis (AS). Methods Between October 2012 and July 2015, a cohort of 112 patients with severe aortic AS underwent 114 BAV procedures as palliative procedure, bridge to definitive treatment or before urgent non-cardiac surgery. Clinical and procedural characteristics, in-hospital outcomes and 2-year mortality data were collected. Kaplan- Meier curves for women and men were constructed and compared by the log-rank test. Multivariable Cox regression analysis was performed to identify the independent predictors of long-term mortality. Results Of the 112 patients, 62.5% (n = 70) were women. As compared with men, women were older, had higher STS score, higher prevalence of chronic kidney disease, arterial hypertension and lower aortic valve area (all p < 0.05). Indications for BAV did not differ by gender. Women had higher risk of vascular complications than man (15.7% vs. 0.0%, p = 0.007) however with similar major periprocedural complications rate (17.1% vs. 9.5%, p = 0.40). Transcatheter aortic valve implantation (TAVI) was performed in 22.8% (n = 16) women and 26.2% (n = 11) men, p = 0.61, surgical aortic valve replacement (AVR) in 10% (n = 7) women and 11.9% (n = 5) men, p = 0.70. Women and men treated with TAVI/AVR had lower mortality as compared with conservative treatment (p = 0.004). Peri-procedural, in-hospital and 24- month mortality before definitive treatment were comparable between women and men (2.9% vs. 2.4%, p = 1.00; 11.4% vs. 4.9%, p = 0.26; 63.3% vs. 39.0%, p = 0.22, respectively). Kaplan-Meier curves for survival stratified by gender are shown in Figure 1. In a multivariable Cox model, STS above 9.8 (HR = 2.29; 95% CI: 1.09–4.83, p = 0.028) was an independent predictor of all-cause mortality only in women. Figure 1 Kaplan-Meier curves for survival stratified by gender Conclusions Presence of significant gender-related differences in baseline characteristics, procedural data and risk of vascular complications were confirmed for patients with severe AS undergoing BAV. However, no difference in major procedural complications as well as long-term mortality between women and men was observed., Background Patent foramen ovale (PFO) with occasional right-to-left shunt is associated with an increased risk of decompression illness (DCI). Divers with a history of repetitive or severe DCI and diagnosed PFO should be considered for the percutaneous closure of the defect if they wish to continue with unrestricted diving. In this study we aimed to summarise our experience in transcatheter PFO closure in professional divers with the history of DCI, based on a long-term follow-up of 11 divers treated in our centre. Methods A follow-up of 11 consecutive divers (9 males, 2 females) in whom percutaneous closure of PFO was performed between 2001–2015 for the secondary prevention of DCI was carried out by phone contact (Table I). Table I Characteristics of the investigated group No. Gender Age [years] Observation time [months] Occluder implanted Time from intervention to next diving [days] Number of divings after intervention Max. depth before intervention [m] Max. depth after intervention [m] 1 Male 41 172 APFO 25 5 10,000* 70 160 2 Female 36 121 APFO 25 7 100 110 70 3 Male 35 115 APFO 25 270 300 55 60 4 Male 32 101 APFO 25 180 700 115 120 5 Male 44 99 CARDIA PFO 30 90 800 60 40 6 Female 35 91 APFO 25 365 30 100 70 7 Male 44 60 APFO 25 90 350 75 90 8 Male 40 60 APFO 25 120 150 80 102 9 Male 49 58 APFO 25 30 1,000 140 140 10 Male 30 40 APFO 25 120 150 80 100 11 Male 47 9 APFO 25 180 30 102 80 * Saturation diving technique: total time (hours) from compression to complete decompression. Results The median follow-up at the time of the analysis was 91 (minimum 9, maximum 172) months. No complications of the procedure were observed in the investigated group. All patients returned to unrestricted, deep diving after the procedure, performing a total of 3,610 dives with the median number of 225 dives (lower quartile value: 82.5, upper quartile value: 72.5 dives). The majority of subjects dived as deep as they did before the intervention, or deeper, achieving mean maximum depth of 93.8 ±35.6 m (vs. 89.73 ±25.92 m before the intervention, p = 0.71). No episode of DCI was observed in the follow-up. Conclusions Percutaneous closure of PFO appears reasonably effective in secondary prevention of DCI associated with intra-cardiac shunt., Background In the light of randomized trials, renal artery stenting (PTA) seems to have questionable effect on clinical outcome. However, smaller observational studies indicated that still there might be a field for intervention in the selected subset of subjects. Therefore, the present study aimed to investigate what features distinguish subjects who improved in terms of the systolic blood pressure (SBP) reduction after PTA from those who did not benefit. Methods Study enrolled 118 (57 male, 63.9 ±8.8 years) consecutive patients referred to PTA for ill-controlled hypertension. Blood pressure control was obtained using 24-hour ambulatory blood pressure monitoring (ABPM), with assessment of the mean SBP and diastolic blood pressure (DBP), the loads of SBP and DBP in patients on antihypertensive drugs, before PTA and 12 months after PTA. Study group was divided retrospectively with regard to achieved SBP control. Subjects in whom SBP decreased by at least 20 mm Hg during 12 month FU after PTA were comprised in group I: 25 (21%) patients. Group II comprised 93 (79%) subjects in whom SBP reduction was lower than 20 mm Hg or deteriorated. Results Analysis showed that group I vs. group II patients had higher renal artery stenosis (RAS) degree (75.2 ±14.7% vs. 66.7 ±10.5%, p = 0.001). The initial SBP, DBP, SBP load and DBP load were significantly higher in group I vs. group II subjects (154.8 ±16.7 mm Hg vs. 130.6 ±16.4 mm Hg, p < 0.001; 86.1 ±11.7 mm Hg vs. 72.7 ±8.8 mm Hg, p < 0.001; 77.9 ±18.7 mm Hg vs. 40 ±28.2 mm Hg, p < 0.001, and 50 ±26.4 mm Hg vs. 21 ±18.5 mm Hg, p < 0.001, respectively). The mean number of blood pressure lowering agents was similar in group I and group II subjects, both before (3.44 ±1.3 vs. 3.26 ±1.1, p = 0.479) and 12 months following PTA (3.12 ±1.4 vs. 2.95 ±1.2, p = 0.541). This may indicate that the effect of blood pressure (BP) values decrease was associated rather with PTA, than drug regiments. On backward logistic multivariable analysis, independent predictors of significant blood pressure reduction after the PTA were: higher RAS degree (OR = 1.24, 95% CI: 1.07–1.44; p = 0.006), higher initial SBP (OR = 1.27, 95% CI: 1.03–1.56; p = 0.026), higher initial DBP load (OR = 1.33, 95% CI: 1.09–1.64; p = 0.007) and bilateral RAS or RAS of single functioning kidney (OR = 1.16, 95% CI: 1.00–1.36; p = 0.053). Conclusions Among patients after PTA, about 20% are high responders with regard to blood pressure control. Positive effect following PTA of RAS can be expected in patients with higher initial SBP and DBP parameters, with high-grade RAS and subjects with bilateral RAS or RAS of single functioning kidney. Our results indicate that PTA of RAS is justified in carefully selected patients., Background Studies indicate that patients with renal artery stenosis (RAS) are at high risk of cardiovascular events (CVE), e.g. myocardial infarction (MI), stroke (IS), and cardiovascular death (CVD). However, the impact of renal artery stenting (PTA) in subjects with RAS on the long-term prognosis and factors associated with this unfavourable prognosis are still debated. The present study aimed to evaluate impact of PTA on renal function, blood pressure and survival in patients treated with PTA for RAS, as well as to identify independent risk factors of CVE in long-term follow-up. Methods Two-hundred-and-forty renal artery angioplasty procedures were performed in 208 consecutive subjects (113 male, mean age: 64.5 ±11.9 years) for the mean RAS degree of 70.3 ±13.4% between June 1997 and December 2015. The immediate and long term outcome of PTA was assessed with regard to renal function, blood pressure values and incidence of restenosis. Prevalence of CVE, including CVD, MI, and IS were recorded prospectively. The potential prognostic risk factors of CVD/MI/IS were analyzed. Results The procedural success was 239/240 procedures. Mean creatinine level decreased from pre-PTA: 133 ±62 to 116 ±55.9 µmol/l at 6 month, and to 117 ±58.6 µmol/l at 12 months after PTA (p = 0.003). Systolic blood pressure changed from 142 ±21.8 mm Hg before PTA to 130 ±15.9 mm Hg, at 6 month, and to 130 ±15 mm Hg at 12 month (p < 0.001), while diastolic blood pressure from 78 ±12.3 to 74 ±9.2 mm Hg, and to 73.7 ±9.5 mm Hg (p < 0.001) respectively. The mean number of blood lowering agents was reduced from 3.33 ±1.2 before PTA to 3.06 ±1.3 at 12 month following PTA (p = 0.003). Restenosis occurred in 24/207 (11.6%) patients and it was treated with repeated PTA. Factors associated with restenosis were: hyperlipidemia (OR = 1.23, 95% CI: 1.06–1.42, p = 0.005) and diabetes (OR = 1.15, 95% CI: 1.0–1.33, p = 0.055). During the mean follow-up period of 62.1 ±37 (range: 1–213) months, CVE occurred in 54 (26%) patients (24 CVDs, 17 non-fatal MIs, 13 non-fatal ISs). Initial serum creatinine (OR = 1.25; 95% CI: 1.04–1.51, p = 0.02) and creatinine at 12 month (OR = 1.18; 95% CI: 1.03–1.35, p = 0.015), concomitant coronary artery disease (OR = 1.19; 95% CI: 1.04–1.36, p = 0.011), and SBP at 12 months after PTA (OR = 1.16; 95% CI: 1.02–1.33, p = 0.025) were independently associated with CVD/MI/IS risk. Conclusions PTA of RAS resulted in significant reduction of creatinine level, blood pressure values and the number of blood lowering agents. During mean 5-year period following PTA of RAS, the CVE rate was 26%. Factors independently associated with CVE risk were pre procedural and 12 month levels of serum creatinine, SBP at 12 months following PTA and concomitant coronary artery disease. Restenosis occurred in 11.6% and it was associated with hyperlipidemia and diabetes., Aim To evaluate the role of carotid artery stenting (CAS) performed according to the ‘tailored’ algorithm in the revascularization of severe internal carotid artery (ICA) stenosis in patients requiring (urgent or elective) cardiac surgery. Methods From 2009 to 2015, 100 patients (69.2 ± 6.7 years, range: 53–84 years; 77% male) underwent CAS combined with cardiac surgery: coronary artery bypass grafting (CABG) and/or valve surgery (Table I). The decision on the type and timing of the procedures was made individually in the vascular heart team. Patients with non-ST elevation myocardial infarction (NSTEMI)/unstable angina/NYHA III–IV had a hybrid procedure (HP): CAS simultaneous with cardiac surgery. Those with severe, but stable cardiac disease had staged procedure (SP): CAS in the first step and cardiac surgery ≥ 5 weeks later. Carotid artery stenting was done according to the ‘tailored’ strategy – to fit proper neuroprotection (proximal or distal) and stent type (open, closed-cell or mesh-covered) to the ICA stenosis severity and neurological symptoms. In the HP group CAS was performed on acetylsalicylic acid and unfractionated heparin; loading dose of clopidogrel (300 mg) was given in ≥ 6th postoperative hour, if major bleedings were excluded. Patients in SP group had dual antiplatelet therapy for a month, clopidogrel was withdrawn 5–7 days before cardiac surgery. Table I Patient and procedure characteristics Parameter HP (N = 58) SP (N = 42) P-value Age [years] 70.2 ±6.6, range: 53-83 67.9 ±6.8, range: 55-84 0.09 Ipsilateral stroke/TIA 17 (29%) 22 (52%) 0.13 ICA stenosis (%) 85.3 ±9.7; 60-99 85.3 ±8.1; 60-99 1.0 Proximal neuroprotection device 28 (48.3%) 21 (50%) 1.0 Closed-cell/mesh-covered stent 55 (95%) 37 (88%) 0.88 Left main stenosis 21 (36%) 12 (28.6%) 1.0 EuroSCORE II 2.74 ±1.39; 0.95-6.91 1.98 ±1.08; 0.85-4.95 0.004 Isolated CABG 52 (89.6%) 36 (86%) 0.88 Isolated valve surgery or CABG + other procedure* 6 (10.3%) 6 (12.5%) 0.75 * Valve/aortic surgery/left ventricle aneurysmectomy/ left atrial appendage closure. Results No major neurological in hospital or 30-day complications were noted. In the early postoperative period 1 myocardial infarction and 2 deaths as a result of multi-organ failure (3; 5.2%) occurred in the HP group and 1 (2.4%) death due to cardiogenic shock in SP group; p = 0.6. Conclusions Carotid artery stenting performed according to the ‘tailored’ algorithm prior to cardiac surgery is a low risk procedure and might be an option to prevent possible perioperative neurological complications of ICA stenosis. Patients requiring urgent cardiac surgery have slightly increased risk of the procedure., Background About 30% of patients with carotid artery stenosis (CAS) develop dementia after cerebral ischemic event (CIE), and 20–50% suffer from CIE recurrence during 6 months. Carotid artery revascularization (CAR) may prevent CIE recurrence, at the cost of new micro-embolic lesions (MES). The impact of CAR on cognitive function is debatable. The aim was to assess either functional and cognitive outcome, cerebral flow on transcranial Doppler (TCD) and brain magnetic resonance imaging (MRI) in patients with symptomatic CAS referred to CAR. Methods Fifteen patients (aged 68 ±8.2 years old, 9 male) with recent CIE (17.6 ±10.2 days to CAR) related to CAS of mean 88.9 ±3.9% lumen reduction were prospectively with TCD, diffusion and perfusion MRI, Montreal Cognitive Assessment (MoCA), Mini Mental State Examination (MMSE), modified Rankin Scale (mRs) and National Institutes of Health Stroke Scale (NIHSS) 24 h before, at 48–72 h and 1 month following CAR. Results New MES were found in 7 (46.7%) subjects following CAR. Carotid artery revascularization resulted in significant increase of cerebral flow velocity in middle and anterior cerebral arteries (p < 0.001 and p = 0.009; respectively) and cerebral perfusion measured by TTP and MTT (p = 0.005 and p = 0.002; respectively). Neurologic tests showed improvement in NIHSS (3.1 ±1.1 to 1.9 ±1.2; p = 0.003), mRs (from 1.6 ±0.8 to 0.9 ±0.9, p = 0.008), and MMSE (26.4 ±2.4 to 27.8 ±2.3; p = 0.012) at 1 month. Montreal Cognitive Assessment scores before and 1 month after CAR remained similar (22.8 ±3.6 vs. 23.4 ±3.3; p = 0.752). Conclusions Improvement of cerebral flow and perfusion, functional outcome, as well as no cognitive decline are observed after CAR for symptomatic CAS., Background Very early discharge after percutaneous coronary interventions (PCI) is now possible vascular closure devices (VCDs) when using the femoral access. Methods We retrospectively analyzed 261 consecutive procedures performed from femoral access with a VCD in a single center between January 2008 and December 2013. The primary endpoint was access site complication rate. The secondary endpoint was time from end of procedure to discharge. Results The complication rate was 2.68% (7/261). The following complications occurred: 3 hematomas, 1 pseudoaneurysm requiring treatment and 3 device failures. Mean time to discharge was 4 h 1 min (range: 2 h 19 min to 6 h 48 min). Conclusions Utilization of VCDs allows for safe and early same-day discharge of patients undergoing planned PCI procedures., Background Previous studies of same-day discharge after percutaneous coronary intervention (PCI) demonstrated favorable short term outcomes. There are limited data evaluated the long term results, especially for unselected population. Methods We retrospectively studied and followed up for 12 months 396 consecutive patients undergoing planned PCI with same-day discharge between December 2007 and May 2015 in two stand-alone centers. Results The mean age of the study population was 56.6 ±7.59 years (males 77.22%). 10.1% of the lesions were on bifurcation, 4.29% were CTO, 5.3% were in a bypass graft and 1.26% were in protected LM. Procedural success occurred in 96.21%. Groin access was used in 83.08% with VCD in 93.01% of procedures. Access site complications (uncomplicated VCD failure, hematoma, small dissection, pseudoaneurysm, spasms of radial) occurred in 1.77% of cases. Only 1 patient required hospitalization due to anaphylactic drug reaction in a peri-procedural period. The mean time from procedure end to discharge was 3 h 59 min. No death or major bleeding occurred during 30-day observation. Two patients had early stent thrombosis with MI, 1 patient was re-admitted for repeat angiography without intervention, 1 had TVR and one patient had stroke. At 12 months there were 15 ISR required angioplasty or CABG and 28 TVR. One patient had stroke and one had transient ischemic attack. Seven patients had MI at 12 months (1.77%). One patient died due to non-cardiac reasons. Conclusions Our study shows excellent long term safety outcomes for unselected population of patients undergoing elective PCI in stand-alone centers without overnight stay., Background We aimed to evaluate safety and efficacy of hemostatic polyurethane matrix (Hematrix) for femoral artery access site closure. Methods and results In a series of 31 patients two sizes of Hematrix patch were used: 10 × 10 cm (body mass index > 30 kg/m2 or thick layer of fat at puncture site) or 5 × 5 cm. The arterial sheath (6 F in all cases) was removed directly after the procedure and then the access site was manually compressed for 10 min. In patients who had PCI (and heparin administered) a compression band was placed for 6 h. In the remaining cases no compression band was used, and the access site was checked for hematoma or bleeding during the first 6 h. Hemostasis was obtained in all patients. The blood flow in femoral artery (evaluated by ultrasound examination) was normal in all cases. Only one serious complication was seen – a pseudoaneurysm of femoral artery access site – probably due to administration of low molecular weight heparin 48 h after the procedure. Two cases of mild complications (6% of the group) were seen: superficial hematomas that required prolonged compression by pressure band. Conclusions The Hematrix patch appears to be efficient in closing femoral artery access site after cornarography or PCI. It offers similar advantages and has equally low incidence of complications as currently available mechanical closure devices (sheath removed directly after the procedure, short bed rest or compression time). It is distinguished from them by much lower price and requires less training.

There are at least several ways of detecting bovine heat, which vary in accuracy, as well as in the effort and cost associated with the implementation and operation of equipment designed for this purpose. Heat in cows can be most easily detected by thorough and systematic observation of animals in the herd. Other methods include the measurements of vaginal mucosal resistance, the fitting of balloons with paint at the base of the tail or on the back, temperature measurements in the vagina, rumen and milk, and determination of progesterone levels in milk and blood. Currently, controlled breeding programmes, including timed artificial insemination (TAI) and automated estrus detection (AED) or activity monitoring system (AMS), are routinely introduced in high-performance dairy herds. Most of the commercially available heat detection devices are based on accelerometers or pedometers. These devices are capable of detecting heat in 81.4% to 91.3% of cows. The sensitivity of heat detection was 58.9% for accelerometers (Heatime), 63.3% for pedometers (SAE Afikim, Kibbutz Afikim, Israel), 56.7% for KaMaR markers and 35.9% for heat detectors (Scratchcard). When two of the three systems were combined (different combinations), the sensitivity increased to 75.9%. Similarly, after adding visual detection, sensitivity increased to 96% and specificity to 90% (for visual observation combined with AMS). Activity monitoring systems are a proven tool for improving the management of cows. They are an alternative to reproductive management based solely on traditional methods of detecting ovulation or hormone programmes (TAI) and they respect the well-being of animals. It seems, however, that further improvement in cows’ fertility on industrial cattle farms will be achieved by combining several different methods of heat detection and synchronization, rather than by using a single modern heat detection system..

Pyometra, which is accompanied by bacterial contamination of the uterus, is defined as a complex disease associated with the activation of several systems, including the immune system. The objective of the study was to evaluate the gene expression profile in dogs with pyometra compared with those that were clinically normal. The study included uteri from 43 mongrel bitches (23 with pyometra, 20 clinically healthy). RNA used for the microarray study was pooled to four separated vials for control and pyometra. A total of 17,138 different transcripts were analyzed on the uteri of female dogs with pyometra and of healthy controls. From 264 inflammatory response-related transcripts, we found 23 transcripts that revealed a 10- to 77-fold increased expression. Thereby, the expression of interleukin 8 (IL8), interleukin-1-beta (IL1B), interleukin 18 receptor (IL18RAP), interleukin 1-alpha (IL1A), interleukin receptor antagonist (IL1RN) and interleukin 6 (IL6) increased 77-, 20-, 17-, 13-, 13- and 11-fold, respectively. Furthermore, the expression of the calcium binding proteins S100A8 was 44-fold higher, and that of S100A12 and S100A9 37-fold, respectively, in the uteri of canines with pyometra compared with that of the controls. Moreover, the expression of the transcripts of toll-like receptors (TLR8 and TLR2), integrin beta 2 (ITGB2), chemokine ligand 3 (CCL3), semaphorin 7A (SEMA7A), CD14 and prostaglandin-endoperoxide synthase 2 (PTGS2) was increased between 10- and 18-fold. Furthermore, after using RT-qPCR we found an increased expression of AOAH, IL1A, IL8, CCL3, IL1RN and SERPINE 1 mRNAs which can be served also as markers of the occurrence of pyometra in domestic bitches. In summary, it is concluded that up-regulation of interleukins may be used as a marker of the inflammatory response in dogs with pyometra. Moreover, all of the 23 up-regulated transcripts may be novel molecular markers of the pathogenesis of canine pyometra. Several proteins--–products of these genes--may be recognized as potential biomarkers of this disease or as therapeutic targets in other mammalian species, including humans.

SummaryThis study was aimed at investigating zona pellucida glycoproteins (ZP) ZP2, ZP3 mRNA expression as well as ZP3, ZP4 (ZPB) protein distribution before and afterin vitromaturation (IVM) in canine oocytes. The cumulus–oocyte complexes (COCs) were recovered from 27 anoestrous mongrel bitches and matured for 72 h in TCM199 medium. The canine COCs were analysed before and after IVM. Using real-time quantitative polymerase chain reaction (RQ-PCR), both groups of oocytes were analysed for detection of ZP2 and ZP3 mRNA profiles as well as using confocal microscopic analysis for observation of ZP3 and ZP4 protein distribution. In post-IVM canine oocytes an increase in transcript content of ZP2 and ZP3 genes as well as a decrease in ZP3 and ZP4 protein levels were observed when compared with pre-IVM oocytes. Moreover, the ZP4 protein before IVM was significantly distributed in the peripheral area of cytoplasm, whereas after IVM it was localized rather than in the entire cytoplasm. In contrast, the ZP3 protein was found both before and after IVM was distributed in the peripheral area of the cytoplasm. In conclusion, we suggest that the expression of ZP2 and ZP3 genes is associated with the maturation stage of canine oocytes, as higher mRNAs levels were found after IVM. However, a decreased expression of ZP3 and ZP4 proteins after IVM suggests maturation-dependent down-regulation of these protein translations, which may result in disturbed fertilization.