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201. Molecular surveillance of pandemic influenza A(H1N1) viruses circulating in Italy from May 2009 to February 2010: association between haemagglutinin mutations and clinical outcome

Author

Puzelli, S, Facchini, M, De Marco, M. A, Palmieri, A, Spagnolo, D, Boros, S, Corcioli, F, Trotta, D, Bagnarelli, P, Azzi, Anna, Cassone, A, Rezza, G, Pompa, M. G, Oleari, F, Donatelli, I, Capobianchi, Mr, Fadda, Giuseppe, Palu', Giorgio, Vitale, F, D'Agaro, P, Esposito, C, Ghisetti, V, Ansaldi, F, Zanetti, A, Nelli, Lc, Baldanti, F., Puzelli, S., Facchini, M., De Marco, M. A., Palmieri, A., Spagnolo, D., Boros, S., Corcioli, F., Trotta, D., Bagnarelli, P., Azzi, A., Cassone, A., Rezza, G., Pompa, M. G., Oleari, F., Donatelli, I., Influnet Surveillance Group for Pandemic A., 2009 Influenza Virus in Italy, D'Agaro, Pierlanfranco, Puzelli, S, Facchini, M, De Marco, MA, Palmieri, A, Spagnolo, D, Boros, S, Corcioli, F, Trotta, D, Bagnarelli, P, Azzi, A, Cassone, A, Rezza, G, Pompa, MG, Oleari, F, Donatelli, I, and Vitale, F

Subjects
Male, Molecular surveillance, Pandemic influenza A(H1N1), Haemagglutinin mutations, Italy from May 2009 to February 2010, pandemic influenza, surveillance of pandemic influenza A(H1N1), Epidemiology, viruses, Settore MED/42 - Igiene Generale E Applicata, medicine.disease_cause, Severity of Illness Index, Influenza A Virus, H1N1 Subtype, Pandemic, Influenza A Virus, A(H1N1), Child, Mutation, Reverse Transcriptase Polymerase Chain Reaction, Transmission (medicine), Adolescent, Adult, Age Distribution, Aged, Amino Acid Substitution, Child, Preschool, Female, Hemagglutinins, Humans, Infant, Influenza, Human, Italy, Middle Aged, Population Surveillance, Sex Distribution, Young Adult, Pandemics, haemagglutinin mutations, Human, Biology, Disease cluster, Disease course, Virology, medicine, H1N1 Subtype, Preschool, Molecular epidemiology, Public Health, Environmental and Occupational Health, Pandemic influenza, Influenza, Mutational analysis
Abstract

Haemagglutinin sequences of pandemic influenza A(H1N1) viruses circulating in Italy were examined, focusing on amino acid changes at position 222 because of its suggested pathogenic relevance. Among 169 patients, the D222G substitution was detected in three of 52 (5.8%) severe cases and in one of 117 (0.9%) mild cases, whereas the D222E mutation was more frequent and evenly distributed in mild (31.6%) and severe cases (38.4%). A cluster of D222E viruses among school children confirms reported human-to-human transmission of viruses mutated at amino acid position 222.

Published
2010

202. Multilocus microsatellite polymorphism analysis to characterize Leishmania infantum strains isolated in Sicily

Author

Reale S., Lupo T., Migliazzo A., Di Mauro C., Ciprì V., Calderone S., Vitale F., MANNA, LAURA, Reale, S., Lupo, T., Migliazzo, A., Di Mauro, C., Ciprì, V., Calderone, S., Manna, Laura, and Vitale, F.

Subjects
Dogs, Polymorphism, Genetic, Animals, Dog Diseases, Leishmania infantum, Sicily, Microsatellite Repeats
Abstract

Different approaches are being developed to improve the differentiation of Leishmania genus using biochemical and molecular methods. In this study, 11 independent polymorphic microsatellites were used for the typing of strains of L. infantum isolated in Sicily. Polymerase chain reaction was employed to amplify the microsatellites contained in 12 DNA regions selected from among more investigated loci. A total of 51 isolates of L. infantum from dogs were tested by using the same locus panel. The products were successively analysed using an automatic sequence detector (ABI PRISM 3130 AB), to discover relevant microsatellite polymorphisms. It was possible to discriminate between MON-1 and non-MON-1 groups. Moreover, the method permitted to distinguish various genotypes of L. infantum isolates within each zymodema. Model- and distance-based analyses of the data set showed comparable results. The frequency of heterozygosity in the alleles analysed varied extremely between the different groups of isolates. As the method exhibits a high level of discrimination, it is suitable for characterization of closely related strains in epidemiological studies.

Published
2010

203. Evidence for a relationship between Leishmania load and clinical manifestations

Author

MANNA, LAURA, GRAVINO, ANGELO ELIO, Reale S., Picillo E., Vitale F., Manna, Laura, Reale, S., Picillo, E., Vitale, F., and Gravino, ANGELO ELIO

Abstract

Visceral leishmaniasis (VL) is a life-threatening disease of medical, social and economic importance in endemic areas. Dogs are the main reservoir of Leishmaniainfantum. In this study, the authors investigated a group of 56 natural infected dogs to establish the relationship between parasite load and various clinical forms of leishmaniasis. The sick dogs were monitored at the beginning from clinical and physiological point of view. Leishmania load was measured by real-time PCR assay on whole blood samples and lymph node aspirates, collected at the time of diagnosis. Our results indicate that a higher quantity of Leishmania DNA was found in the lymph nodes of dogs characterized by maximum clinical score. This interesting finding indicates the presence of a positive relationship between Leishmania load and clinical manifestations in dogs showing a severe clinical form of leishmaniasis.

Published
2009

205. High-frequency noise modeling of Si(Ge) bipolar transistors

Author

Vitale, F. and Heemink, A.W.

Subjects
parameter extraction, noise, non quasi static effects, circuit simulations, base resistance, modeling, compact modeling, bipolar transistors
Abstract

The design and the optimization of electronic systems often requires a detailed knowledge of the inherent noise generated within semiconductor active devices, constituting the core of such systems. Examples of applications in which noise is a key issue include receiver front-ends in radiofrequency (RF) and optoelectronic transmission systems, front-end stages in sensors and also oscillators and mixers. The rapid growth of the silicon- (Si-) based RF electronics has triggered increasing interest towards low-noise Si-based devices, such as silicon-germanium (SiGe) heterojunction bipolar transistors (HBT's). In this context, modeling of the high-frequency noise can be considered an essential tool for the optimization of noise performance at both technological level (device design) and circuit level (circuit design). This thesis addresses the problem of high-frequency noise modeling in Si(Ge) bipolar transistors. As remarked throughout the treatise, accurate and reliable noise predictions must rely on a careful analysis of the carrier transport in the intrinsic transistor as well as of the impact of the parasitics constituting the extrinsic part of the device. The relative impact of the extrinsic and intrinsic part on noise characteristics depends upon bias conditions. Chapter 2 begins with an experimental demonstration of the relevance of the extrinsic network to simulated noise characteristics, through a study of the impact of the base resistance distribution with respect to the base-collector capacitance. Furthermore, approximated analytical results for the real part of the feedback admittance y12 are presented, in terms of the small-signal equivalent circuit of the Mextram compact model. Such analytical results provide a method for the extraction of the base resistance distribution. From Chapter 3 on, the analysis steps into the intrinsic device level, focusing on the transport process of the minority carriers injected into the quasi-neutral base region. Chapter 3 provides a thorough review of selected most seminal contributions to the noise modeling theory of bipolar devices, namely Van der Ziel's collective approach, Polder-Baelde's equivalent network and Van Vliet's analytical approach based on the Green's function approach. Chapter 3 serves as theoretical basis for the subsequent chapters. In Chapter 4 a noise modeling approach is presented based on a lumped network. Such network is the result of a discretization of the partial differential equations governing the transport process of minority carriers injected into the quasi-neutral base region. The proposed approach is first verified based on analytical reasoning and then through the assessment of noise characteristics of a complete industrial SiGe HBT. Chapter 5 presents a comparative analysis of approximated high-frequency noise model formulations for the intrinsic transistor. The analysis includes the approximated transport noise model feasible for compact model implementations and a correlated noise model derived systematically from theoretical results discussed in Chapter 3, along with the non quasi-static theory of bipolar transistors. In the last part of the chapter a discussion over the impact of the high-frequency effects in the emitter region is profiled.

Published
2014

207. Influenza vaccination coverage among medical residents

Author

Costantino, C., Mazzucco, W., Azzolini, E., Baldini, C., Bergomi, M., Biafior, A., Bianco, M., Borsari, L., Cacciari, P., Cadeddu, C., Camia, P., Carluccio, E., Conti, A., De Waure, C., Di Gregori, V., Fabiani, L., Fallico, R., Filisetti, B., Flacco, M., Franco, E., Furnari, R., Galis, V., Gallea, M., Gallone, M., Gallone, S., Gelatti, U., Gilardui, F., Giuliani, A., Grillo, Orazio Claudio, Lanati, N., Mascaretti, S., Mattei, A., Micò, R., Morciano, L., Nante, N., Napoli, G., Nobile, C., Palladino, R., Parisi, S., Passaro, M., Pelissero, G., Quarto, M., Ricciardi, W., Romano, G., Rustico, E., Saponari, A., Schioppa, F., Signorelli, C., Siliquini, R., Trabacchi, V., Triassi, M., Varetta, A., Ziglio, A., Zoccali, A., Vitale, F., and Amodio, E.

Published
2014

208. Influenza vaccination coverage among medical residents: an Italian multi center survey

Author

Costantino, C, Mazzucco, W, Azzolini, E, Baldini, C, Bergomi, M, Biafiore, Ad, Bianco, M, Borsari, L, Cacciari, P, Cadeddu, C, Camia, P, Carluccio, E, Conti, A, De Waure, C, Di Gregori, V, Fabiani, Leila, Fallico, R, Filisetti, B, Fiacco, Me, Franco, E, Furnari, R, Galis, V, Gallea, Mr, Gallone, Mf, Gallone, S, Gelatti, U, Gilardi, F, Giuliani, Anna Rita, Grillo, Oc, Lanati, N, Mascaretti, S, Mattei, Antonella, Micò, R, Morciano, L, Nante, N, Napoli, G, Nobile, Cg, Palladino, R, Parisi, S, Passaro, M, Pelissero, G, Quarto, M, Ricciardi, W, Romano, G, Rustico, E, Saponari, A, Schioppa, Fs, Signorelli, C, Siliquini, R, Trabacchi, V, Triassi, M, Varetta, A, Ziglio, A, Zoccali, A, Vitale, F, and Amodio, E.

Subjects
medical residents, Italy, vaccination, multicentre survey, coverage rate
Published
2014

209. Cancer incidence in Italian contaminated sites

Author

Comba, Pietro, Ricci, Paolo, Iavarone, Ivano, Pirastu, Roberta, Buzzoni, Carlotta, Fusco, Mario, Ferretti, Stefano, Fazzo, Lucia, Pasetto, Roberto, Zona, Amerigo, Crocetti, Emanuele, Autelitano, M., Beccaloni, E., Benedetti, M., Benfatto, L., Biggeri, A., Binazzi, A., Bianconi, Fortunato, Bidoli, E., Bruno, LAURA CAMILLA, Buzzoni, C., Candela, G., Carere, M., Catelan, D., Cocchioni, M., Comba, P, Conti, S., Corfiati, M., Coviello, Eleonora, Cremone, L., Crocetti, E., Dei Tos, A. P., De Santis, M., Falcini, F., Falleni, F., Fazzo, L., Federico, M., Fusco, M., Giacomin, A., Gola, G., Grisotto, L., Guzzinati, S., Iavarone, I., LA ROSA, Francesco, Lillini, Lucia, Madeddu, A., Magoni, M., Mangone, L., Manno, V., Marcello, I., Marinaccio, A., Marsili, G., Maspero, S., Maule, M., Mazzoleni, Giorgio, Merletti, F., Minelli, G., Minerba, A., Michiara, M., Nicita, C., Pannozzo, F., Pasetto, R., Piccardi, A., Piffer, S., Pirastu, R., Pisani, P., Ricci, P., Santoro, M., Scaini, F., Sciacca, S., Sechi, O., Serraino, D., Soggiu, M. E., Stracci, Fabrizio, Sardo, A. Sutera, Tagliabue, G., Tisano, F., Usala, M., Vercelli, Marina, Vitale, F., Vitarelli, S., Zambon, P., and Zona, A.

Subjects
Contaminated sites, Cancer incidence, Environmental pollution, Public Health, Environmental and Occupational Health, Environmental and Occupational Health, Public Health
Published
2014

210. Cancer incidence in Italian contaminated sites

Author

Comba, P., Ricci, P., Iavarone, I., Pirastu, R., Buzzoni, C., Fusco, M., Ferretti, S., Fazzo, L., Pasetto, R., Zona, A., Crocetti, E., Autelitano, M., Beccaloni, E., Benedetti, M., Benfatto, L., Biggeri, A., Binazzi, A., Bianconi, F., Bidoli, E., Bruno, C., Candela, G., Carere, M., Catelan, D., Cocchioni, Mario, Conti, S., Corfiati, M., Coviello, E., Cremone, L., Dei Tos, A. P., De Santis, M., Falcini, F., Falleni, F., Federico, M., Giacomin, A., Gola, G., Grisotto, L., Guzzinati, S., La Rosa, F., Lillini, L., Madeddu, A., Magoni, M., Mangone, L., Manno, V., Marcello, I., Marinaccio, A., Marsili, G., Maspero, S., Maule, M., Mazzoleni, G., Merletti, F., Minelli, G., Minerba, A., Michiara, M., Nicita, C., Pannozzo, F., Piccardi, A., Piffer, S., Pisani, P., Santoro, M., Scaini, F., Sciacca, S., Sechi, O., Serraino, D., Soggiu, M. E., Stracci, F., Sutera Sardo, A., Tagliabue, G., Tisano, F., Usala, M., Vercelli, M., Vitale, F., Vitarelli, Susanna, and Zambon, P.

Published
2014

211. Confronto fra registri specializzati e registri tumori di popolazione: i risultati del progetto ReNaM-AIRTUM = A comparative analysis between regional mesothelioma registries and cancer registries: results of the ReNaM-AIRTUM project

Author

Nicita, C, Buzzoni, C, Chellini, E, Ferretti, S, Marinaccio, A, Mensi, C, AIRTUM Working Group, ReNaM Working Group, Progetto ReNaM AIRTUM Working Group, Autelitano, M, Bizzoco, S, Bonelli, L, Borciani, E, Candela, G, Cavalieri D’oro, L, Cocchioni, Mario, Coviello, V, Cremone, L, Crocetti, E, Cusimano, R, Dei Tos AP, Falcini, F, Fusco, M, Gennaro, V, Giacomin, A, Gola, G, La Rosa, F, Madeddu, A, Magoni, M, Mangone, L, Marchesi, C, Maspero, S, Mazzoleni, G, Melcarne, A, Merletti, F, Michiara, M, Minerba, S, Pannozzo, F, Piffer, S, Ponz de Leon, M, Quarta, F, Ricci, P, Russo, A, Sampietro, G, Sciacca, S, Sechi, O, Serraino, D, Sutera Sardo, A, Tagliabue, G, Tisano, F, Traina, A, Tumino, R, Usala, M, Vitale, F, Vitarelli, Susanna, Zanetti, R, and Progetto ReNaM AIRTUM Working

Published
2014

213. INCIDENZA DELLE INFLUENZA LIKE-ILLNESSES TRA I MEDICI IN FORMAZIONE SPECIALISTICA ITALIANI: RISULTATI DI UNO STUDIO MULTICENTRICO

Author

Restivo, V, Costantino, C, Amodio, E, Azzolini, E, Baldini, C, Bergomi, M, Biafiore, Ad, Bianco, M, Borsari, L, Cacciari, P, Cadeddu, C, Camia, P, Carluccio, E, Conti, A, De Waure, C, Di Gregori, V, Fabiani, Leila, Fallico, R, Filisetti, B, Flacco, Me, Franco, E, Furnari, R, Galis, V, Gallea, Mr, Gallone, Mf, Gallone, S, Gelatti, U, Gilardi, F, Giuliani, Anna Rita, Grillo, Oc, Lanati, N, Mascaretti, S, Mattei, Antonella, Micò, R, Morciano, L, Nante, N, Napoli, G, Nobile, C, Palladino, R, Parisi, S, Passaro, M, Pelissero, G, Quarto, M, Ricciardi, W, Romano, G, Rustico, E, Saponari, A, Schioppa, Fs, Signorelli, C, Siliquini, R, Trabacchi, V, Triassi, M, Varetta, A, Ziglio, A, Zoccali, A, Calamusa, G, Firenze, A, and Vitale, F.

Published
2014

214. Increase in transmitted resistance to non-nucleoside reverse transcriptase inhibitors among newly diagnosed HIV-1 infections in Europe

Author

Frentz, D. Van de Vijver, D.A.M.C. Abecasis, A.B. Albert, J. Hamouda, O. Jørgensen, L.B. Kücherer, C. Struck, D. Schmit, J.-C. Vercauteren, J. Åsjö, B. Balotta, C. Beshkov, D. Camacho, R.J. Clotet, B. Coughlan, S. Griskevicius, A. Grossman, Z. Horban, A. Kolupajeva, T. Korn, K. Kostrikis, L.G. Liitsola, K. Linka, M. Nielsen, C. Otelea, D. Paraskevis, D. Paredes, R. Poljak, M. Puchhammer-Stöckl, E. Sönnerborg, A. Stanekova, D. Stanojevic, M. Van Wijngaerden, E. Wensing, A.M.J. Boucher, C.A.B. Puchhammer-Stockl, E. Sarcletti, M. Schmied, B. Geit, M. Balluch, G. Vandamme, A.-M. Vercauteren, J. Derdelinckx, I. Sasse, A. Bogaert, M. Ceunen, H. De Roo, A. De Wit, S. Echahidi, F. Fransen, K. Goffard, J.-C. Goubau, P. Goudeseune, E. Yombi, J.-C. Lacor, P. Liesnard, C. Moutschen, M. Pierard, D. Rens, R. Schrooten, Y. Vaira, D. Vandekerckhove, L.P.R. Van den Heuvel, A. Van Der Gucht, B. Van Ranst, M. Vandercam, B. Vekemans, M. Verhofstede, C. Clumeck, N. Van Laethem, K. Demetriades, I. Kousiappa, I. Demetriou, V. Hezka, J. Bruckova, M. Linka, M. Machala, L. Nielsen, C. Jørgensen, L.B. Gerstoft, J. Mathiesen, L. Pedersen, C. Nielsen, H. Laursen, A. Kvinesdal, B. Salminen, M. Ristola, M. Liitsola, K. Suni, J. Sutinen, J. Korn, K. Kücherer, C. Berg, T. Braun, P. Poggensee, G. Däumer, M. Eberle, J. Heiken, H. Kaiser, R. Knechten, H. Müller, H. Neifer, S. Schmidt, B. Walter, H. Gunsenheimer-Bartmeyer, B. Harrer, T. Paraskevis, D. Hatzakis, A. Magiorkinis, E. Hatzitheodorou, E. Haida, C. Zavitsanou, A. Magiorkinis, G. Lazanas, M. Chini, M. Magafas, N. Tsogas, N. Paparizos, V. Kourkounti, S. Antoniadou, A. Papadopoulos, A. Panagopoulos, P. Poulakou, G. Sakka, V. Chryssos, G. Drimis, S. Gargalianos, P. Lelekis, M. Chilomenos, G. Psichogiou, M. Daikos, G.L. Panos, G. Haratsis, G. Kordossis, T. Kontos, A. Koratzanis, G. Theodoridou, M. Mostrou, G. Spoulou, V. Coughlan, S. De Gascun, C. Byrne, C. Duffy, M. Bergin, C. Reidy, D. Farrell, G. Lambert, J. O'Connor, E. Rochford, A. Low, J. Coakely, P. O'Dea, S. Hall, W. Grossman, Z. Levi, I. Chemtob, D. Balotta, C. Riva, C. Mussini, C. Caramma, I. Capetti, A. Colombo, M.C. Rossi, C. Prati, F. Tramuto, F. Vitale, F. Ciccozzi, M. Angarano, G. Rezza, G. Schmit, J.C. Struck, D. Hemmer, R. Arendt, V. Staub, T. Schneider, F. Roman, F. Wensing, A.M.J. Boucher, C.A.B. van Kessel, A. van Bentum, P.H.M. Brinkman, K. op de Coul, E.L. van der Ende, M.E. Hoepelman, I. van Kasteren, M. Juttmann, J. Kuipers, M. Langebeek, N. Richter, C. Santegoets, R. Schrijnders-Gudde, L. Schuurman, R. van de Ven, B.J.M. Åsjö, B. Ormaasen, V. Aavitsland, P. Horban, A. Stanczak, J.J. Stanczak, G.P. Firlag-Burkacka, E. Wiercinska-Drapalo, A. Jablonowska, E. Malolepsza, E. Leszczyszyn-Pynka, M. Szata, W. Camacho, R. Palma, C. Borges, F. Paixão, T. Duque, V. Araújo, F. Jevtovic, D. Salemovic, D. Stanekova, D. Habekova, M. Mokras, M. Truska, P. Poljak, M. Lunar, M. Babic, D. Tomazic, J. Vidmar, L. Vovko, T. Karner, P. Clotet, B. Domingo, P. Galindo, M.J. Miralles, C. del Pozo, M.A. Ribera, E. Iribarren, J.A. Ruiz, L. de la Torre, J. Vidal, F. Garcia, F. Paredes, R. Albert, J. Heidarian, A. Aperia-Peipke, K. Axelsson, M. Mild, M. Karlsson, A. Sönnerborg, A. Thalme, A. Navér, L. Bratt, G. Karlsson, A. Blaxhult, A. Gisslén, M. Svennerholm, B. Bergbrant, I. Björkman, P. Säll, C. Mellgren, Å. Lindholm, A. Kuylenstierna, N. Montelius, R. Azimi, F. Johansson, B. Carlsson, M. Johansson, E. Ljungberg, B. Ekvall, H. Strand, A. Mäkitalo, S. öberg, S. Holmblad, P. Höfer, M. Holmberg, H. Josefson, P. Ryding, U. on behalf of the SPREAD Programme

Abstract

Background: One out of ten newly diagnosed patients in Europe was infected with a virus carrying a drug resistant mutation. We analysed the patterns over time for transmitted drug resistance mutations (TDRM) using data from the European Spread program.Methods: Clinical, epidemiological and virological data from 4317 patients newly diagnosed with HIV-1 infection between 2002 and 2007 were analysed. Patients were enrolled using a pre-defined sampling strategy.Results: The overall prevalence of TDRM in this period was 8.9% (95% CI: 8.1-9.8). Interestingly, significant changes over time in TDRM caused by the different drug classes were found. Whereas nucleoside resistance mutations remained constant at 5%, a significant decline in protease inhibitors resistance mutations was observed, from 3.9% in 2002 to 1.6% in 2007 (p = 0.001). In contrast, resistance to non-nucleoside reverse transcriptase inhibitors (NNRTIs) doubled from 2.0% in 2002 to 4.1% in 2007 (p = 0.004) with 58% of viral strains carrying a K103N mutation. Phylogenetic analysis showed that these temporal changes could not be explained by large clusters of TDRM.Conclusion: During the years 2002 to 2007 transmitted resistance to NNRTI has doubled to 4% in Europe. The frequent use of NNRTI in first-line regimens and the clinical impact of NNRTI mutations warrants continued monitoring. © 2014 Frentz et al.; licensee BioMed Central Ltd.

Published
2014

215. Identificazione dei ceppi di Leishmania mdiante analisi dei microsatelliti

Author

Reale S., Lupo T., Rea S., Migliazzo A., Piazza M., Ciprì V., Vitale F., MANNA, LAURA, GRAVINO, ANGELO ELIO, Società di Diagnostica di Laboratorio Veterinaria, Reale, S., Lupo, T., Manna, Laura, Gravino, ANGELO ELIO, Rea, S., Migliazzo, A., Piazza, M., Ciprì, V., and Vitale, F.

Published
2008

216. Identification of microbial taxa involved in cultural heritage deterioration and able to produce health hazardous substances by molecular techniques

Author

PALLA, Franco, Vitale F, Reale S., and Palla Franco, Vitale F, Reale S

Subjects
detection, molecular techniques, health hazardous substances, biodeterioration, Settore BIO/11 - Biologia Molecolare
Abstract

Fungi and bacterial, wide-spread in biosphere environments, are the main microorganisms related to the deterioration of cultural assets but, moreover, complex microbial communities may emit mixed aerosol into indoor air. In this study the microbial colonization is investigated from to point of view, conservation of cultural heritage and related potential illness to visitors or professionals. The sampling was performed by non-destructive procedures on works of art surfaces, and by the gelatin membrane filter method (Sartorius) for aerosol. The identification of microbial taxa was performed by molecular analyses based on PCR, sequencing, sequence comparison techniques and, particularly for fungi identification, we report the development of a microarray method. Specific oligonucleotide probes targeted to internal transcribed spacer of rRNA genes, were designed in order to set up a rapid and simple identification method. We focused the attention on fungal species, related to cultural asset deterioration and able to produce and release toxic substances. Under this light, the characterization of microbial consortium and the related metabolic activities are essential for understanding if the conservation procedure is convenient, outlining the possible risk for both works of arts and operators/visitors.

Published
2008

217. Urine sampling for real-time polymerase chain reaction based diagnosis of canine leishmaniasis

Author

MANNA, LAURA, GRAVINO, ANGELO ELIO, REALE S, PICILLO E, VITALE F, Manna, Laura, Reale, S, Picillo, E, Vitale, F, and Gravino, ANGELO ELIO

Abstract

In this study, a real-time PCR assay was exploited for detecting and quantitating Leishmania infantum DNA in urine samples from naturally infected dogs. Forty-one dogs of different breeds and age, showing symptoms compatible with the infection, were employed in the investigation. The dogs were divided into three groups on the basis of the following criteria: 22 dogs showing only cutaneous signs (group 1), 12 dogs showing hematuria but no cutaneous signs (group 2), 7 dogs affected by severe nephropathy (group 3). Three healthy dogs living in the area of Abruzzo Region (Italy) where the disease is not endemic were selected as negative control. Infected dogs belonging to both group2 and 3 showed altered laboratoryparameters related to an impairment of renal function, eventhough were more pronunced in the group 3 of dogs.The real-time PCR analysis showed higher levels of Leishmania DNA in the lymph node aspirates from all the groups of infected dogs than those measured in their blood or urines. Interestingly, urine samples from dogs belonging to group 2 and 3 contained an higher Leishmania DNA load than that detected in their blood. This finding suggests that a real-time PCR analysis of urines from infected dogs could be useful and non invasive tool for monitoring the severity of the disease.

Published
2008

219. Sorveglianza della mortalità infantile nella provincia di Palermo. Dati prelminari

Author

Mazzucco, W., Cusimano, R., LA SCOLA, C., Sammarco, S., Casuccio, N., Pitarresi, A., Scondotto, S., Dardanoni, G., Cassata, N., Corsello, G., Vitale, F., MAZZUCCO W, CUSIMANO R, LA SCOLA C, SAMMARCO S, CASUCCIO N, PITARRESI A, SCONDOTTO S, DARDANONI G, CASSATA N, CORSELLO G, and VITALE F

Subjects
mortalità infantile, sorveglianza, cause di morte, Settore MED/42 - Igiene Generale E Applicata
Published
2007

221. Il metodo Joint Commission International applicato nell’ambito del Progetto Biennale per 'La gestione del Rischio Clinico presso le strutture del Sistema Sanitario della Regione Siciliana: risultati preliminari'

Author

Mazzucco, W., Cantaro, P., Saglimbeni, G., Parrinello, V., Banks, H., Zangrandi, A., Urso, S., Vitale, F., Romano, N., Mazzucco, W, Cantaro, P, Saglimbeni, G, Parrinello, V, Banks, H, Zangrandi, A, Urso, S, Vitale, F, and Romano, N

Subjects
joint commission, accreditamento, gestione rischio clinico
Published
2007

222. Cats as reservoir of Babesia microti

Author

Pennisi, Maria Grazia, Alongi, A, Agnone, A, Vitale, F, Reale, S, Torina, A., Pennisi, MG, Alongi, A, Agnone, A, Vitale, F, Reale, R, and Torina, A

Subjects
Babesia microti
Published
2007

229. Sorveglianza della mortalità infantile nella Provincia di Palermo

Author

Cusimano, R., Mazzucco, W., LA SCOLA, C., Sammarco, S., Casuccio, N., Pitarresi, A., Scondotto, S., Dardanoni, G., Cassata, N., Corsello, G., Vitale, F., CUSIMANO R, MAZZUCCO W, LA SCOLA C, SAMMARCO S, CASUCCIO N, PITARRESI A, SCONDOTTO S, DARDANONI G, CASSATA N, CORSELLO G, and VITALE F

Subjects
registri tumori, mortalità infantile, sorveglianza
Published
2006

232. Associations of classic Kaposi sarcoma with common variants in genes that modulate host immunity

Author

Brown, E. E., Fallin, D., Ruczinski, I., Hutchinson, A., Staats, B., Vitale, F., Lauria, C., Serraino, D., Rezza, G., Mbisa, G., Whitby, D., Messina, A., Goedert, J. J., Chanock, S. J., Romano, N., Ajello, F., Bonura, F., Perna, A. M., Viviano, E., Tramuto, F., Villafrate, M. R., Di Benedetto, M. A., Tamburini, M., Montella, M., Crispo, A., de Sicato, S., de Marco, M. R., Ascierto, P., Piselli, P., Valdarchi, C., Farchi, F., Corona, R. M., Di Carlo, A., Castilletti, C., Gafa, L., Stella, S., Massimino, M., Kroner, B., Chatterjee, N., Chen, J., Kiley, M., Chen, R., BROWN EE, FALLIN D, RUCZINSKI I, HUTCHINSON A, STAATS B, VITALE F, LAURIA C, SERRAINO D, REZZA G, MBISA G, WHITBY D, MESSINA A, GOEDERT JJ, CHANOCK SJ, KAPOSI SARCOMA WORKING GROUP, and Tramuto, F.

Subjects
Adult, Male, Genotype, Epidemiology, Population, Single-nucleotide polymorphism, Biology, Settore MED/42 - Igiene Generale E Applicata, IL12A, medicine, Humans, Genetic Predisposition to Disease, Risk factor, education, Sarcoma, Kaposi, Aged, Aged, 80 and over, education.field_of_study, Classic Kaposi Sarcoma, Polymorphism, Genetic, Case-control study, Cancer, Herpesvirus Infection, Odds ratio, Middle Aged, medicine.disease, Oncology, Haplotypes, Italy, Genetic Variant, Case-Control Studies, Immunology, Herpesvirus 8, Human, Cytokines, Female
Abstract

Classic Kaposi sarcoma (CKS) is an inflammatory-mediated neoplasm primarily caused by Kaposi sarcoma–associated herpesvirus (KSHV). Kaposi sarcoma lesions are characterized, in part, by the presence of proinflammatory cytokines and growth factors thought to regulate KSHV replication and CKS pathogenesis. Using genomic DNA extracted from 133 CKS cases and 172 KSHV-latent nuclear antigen-positive, population-based controls in Italy without HIV infection, we examined the risk of CKS associated with 28 common genetic variants in 14 immune-modulating genes. Haplotypes were estimated for IL1A, IL1B, IL4, IL8, IL8RB, IL10, IL12A, IL13, and TNF. Compared with controls, CKS risk was decreased with 1235T/−1010G–containing diplotypes of IL8RB (odds ratio, 0.49; 95% confidence interval, 0.30-0.78; P = 0.003), whereas risk was increased with diplotypes of IL13 containing the promoter region variant 98A (rs20541, alias +130; odds ratio, 1.88; 95% confidence interval, 1.15-3.08; P = 0.01) when considered in multivariate analysis. Risk estimates did not substantially vary by age, sex, incident disease, or disease burden. Our data provide preliminary evidence for variants in immune-modulating genes that could influence the risk of CKS. Among KSHV-seropositive Italians, CKS risk was associated with diplotypes of IL8RB and IL13, supporting laboratory evidence of immune-mediated pathogenesis. (Cancer Epidemiol Biomarkers Prev 2006;15(5):926–34)

Published
2006

233. Therapy monitoring by real time PCR in patients with Brucellosis

Author

Francesco Scarlata, Titone Lanza Di Scalea, L., Rubino, R., Reale, S., Iaria, C., Antonio Cascio, Vitale, F., Rubino, F., SCARLATA, F, TITONE LANZA DI SCALEA, L, RUBINO, R, REALE, S, IARIA, C, CASCIO, A, VITALE, F, and Rubino, F.

Published
2006

234. LEISHMANIA DNA CHARACTERIZATION IN BIOLOGICAL SAMPLES

Author

Reale S., Vitale F., Pennisi M. G., Vitale M., Polizzi D., Caracappa S., MANNA, LAURA, GRAVINO, ANGELO ELIO, Reale, S., Vitale, F., Pennisi, M. G., Manna, Laura, Gravino, ANGELO ELIO, Vitale, M., Polizzi, D., and Caracappa, S.

Published
2006

238. Correlates of Human Herpesvirus-8 DNA detection among adults in Italy without Kaposi sarcoma

Author

Brown, E. E., Whitby, D., Vitale, F., Fei, P. C., Del Carpio, C., Marshall, V., Alberg, A. J., Serraino, D., Messina, A., Gafa, L., Goedert, J. J., Romano, N., Ajello, F., Bonura, F., Perna, A. M., Viviano, E., Tramuto, F., Villafrate, M. R., Di Benedetto, M. A., Tamburini, M., Montella, M., Crispo, A., De Sicato, S., De Marco, M. R., Ascierto, P., Piselli, P., Rezza, G., Valdarchi, C., Corona, R. M., Giuliani, M., Castilletti, C., Lauria, C., Laura, C., Stella, S., Massimino, M., Kroner, B., Biggar, R. J., Rabkin, C. S., BROWN EE, WHITBY D, VITALE F, CORDIALI FEI P, DEL CARPIO C, MARSHALL V, ALBERG AJ, SERRAINO D, MESSINA A, GAFA L, GOEDERT JJ, THE CLASSICAL KAPOSI SARCOMA WORKING GROUP, and Tramuto, F.

Subjects
Male, Epidemiology, medicine.medical_treatment, Comorbidity, Settore MED/42 - Igiene Generale E Applicata, medicine.disease_cause, Kaposi sarcoma herpesvirus, 80 and over, Leukocytes, Gammaherpesvirinae, Medicine, HHV8, Viral, Aged, 80 and over, education.field_of_study, biology, virus diseases, Immunosuppression, General Medicine, Herpesviridae Infections, Middle Aged, Viral Load, Italy, Herpesvirus 8, Human, Female, Antibody, Kaposi sarcoma herpesviru, Viral load, Human, Adult, Viral DNA, Population, Mononuclear, KSHV, Peripheral blood mononuclear cell, Herpesviridae, Age Distribution, Antigen, Humans, Herpesvirus 8, Sex Distribution, education, Aged, business.industry, Hemodynamics, DNA, biology.organism_classification, Blood Cell Count, Socioeconomic Factors, Immunology, DNA, Viral, biology.protein, Leukocytes, Mononuclear, business
Abstract

Background: The presence of Human Herpesvirus-8 (HHV8) DNA is predictive of Kaposi sarcoma (KS) among patients with HIV-associated or iatrogenic immunosuppression. However, correlates of HHV8-DNA detection in the general population remain undefined. Methods: We assessed correlates of HHV8-DNA detection among Italian adults without KS who had antibodies against HHV8-latent nuclear antigen by immunofluorescence assay. HHV8-K6 DNA sequences were detected in peripheral blood mononuclear cells using TaqMan CR. Results: Of the 158 subjects 26 (16.5%) had detectable HHV8-DNA [median copies/million cells, 53; (13-2128)]. Adjusted for age, sex, and laboratory, HHV8-DNA was detected more frequently in participants with >7 total residents in the childhood home [OR = 3.7 (1.5-9.1)], >2 younger siblings [OR = 2.6 (1.1-6.5)], and current cardiovascular [OR = 3.6 (1.3-9.7)] or renal [OR = 3.1 (1.2-8.0)] disease. Excluding the participants using immune modulating drugs, HHV8-DNA was more frequent among those with low red blood cells (RBC) [92 μm3/red cell; OR = 2.8 (1.0-7.8)], and mild thrombocytopenia [

Published
2005

241. Risultati preliminari del monitoraggio di alcuni parametri biochimici pre e post partum nella vacca in relazione alla produzione lattea ed alla temperatura ambientale

Author

PELI, ANGELO, GENTILINI, FABIO, INGRA', LAURA, FORMIGONI, ANDREA, FAMIGLI BERGAMINI, PAOLO, Cascone G., Lo Magno G., Vitale F., Caracappa S., A. PELI, Peli A., Cascone G., Gentilini F., Lo Magno G., Ingrà L., Vitale F., Formigoni A., Caracappa S., and Famigli-Bergamini P.

Subjects
VACCA DA LATTE, PRODUZIONE, TEMPERATURA AMBIENTALE, CHIMICA CLINICA
Abstract

La presente nota costituisce un rapporto preliminare sui primi risultati ottenuti nell'ambito di un ampio programma di studio in corso nella provincia di Ragusa (Sicilia), condotto congiuntamente all'Istituto Zooprofilattico Sperimentale della Sicilia, dalla Facoltà di Medicina Veterinaria di Teramo, sull'effetto dello "stress da calore" nella vacca da latte. Sono illustrate ed analizzate statisticamente le cinetiche di 21 paramentri ematobiochimici misurati settimanalmente, nelle 4 settimane pre partum e post partum, in 21 bovine di razza frisona italiana. Sono altresì analizzatele cinetiche ematiche dei suddetti parametri in base all'entità della produzione di latte nel primo e nel secondo mese di lattazione. Infine viene riportata l'analisi sulla relazione tra temperatura ambientale, registrata nell'arco dell'intera prova (147gg) e la produzione di latte.

Published
2005

246. A rare mutation in MYH7 gene occurs with overlapping phenotype

Author

Ruggiero, L, Fiorillo, C, Gibertini, S, De Stefano, F, Manganelli, F, Iodice, R, Vitale, F, Zanotti, S, Galderisi, M, Mora, M, Santoro, L, Santoro, L., GIBERTINI, SARA, ZANOTTI, SIMONA, Ruggiero, L, Fiorillo, C, Gibertini, S, De Stefano, F, Manganelli, F, Iodice, R, Vitale, F, Zanotti, S, Galderisi, M, Mora, M, Santoro, L, Santoro, L., GIBERTINI, SARA, and ZANOTTI, SIMONA

Abstract

Mutations in the beta-myosin heavy chain gene (MYH7) cause different muscle disorders. The specific molecular pathobiological processes that cause these different phenotypes remains unexplained. We describe three members of a family with an autosomal dominant mutation in the distal rod of MYH7 [c.5401G> A (p.Glu1801Lys)] displaying a complex phenotype characterized by Laing Distal Myopathy like phenotype, left ventricular non compaction cardiomyopathy and Fiber Type Disproportion picture at muscle biopsy. We suggest that this overlapping presentation confirm the phenotypic variability of MYH7 myopathy and may be helpful to improve the genotype phenotype correlation.

Published
2015

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