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557 results on '"Urata, Yasuo"'

203. Abstract LB-276: A novel approach using the telomerase-selective adenoviral marker (OBP-401) for detection of circulation tumor cells in breast cancer patients

Author

Kim, Seung Jin, primary, Sato, Jun, additional, Taguchi, Tetsuya, additional, Tamaki, Yasuhiro, additional, Tsukamoto, Fumine, additional, Akazawa, Kenji, additional, Masago, Akinori, additional, Tsujino, Yukio, additional, Urata, Yasuo, additional, and Noguchi, Shinzaburo, additional

Published
2010
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205. A Phase I Study of Telomerase-specific Replication Competent Oncolytic Adenovirus (Telomelysin) for Various Solid Tumors

Author

Nemunaitis, John, primary, Tong, Alex W, additional, Nemunaitis, Michael, additional, Senzer, Neil, additional, Phadke, Anagha P, additional, Bedell, Cynthia, additional, Adams, Ned, additional, Zhang, Yu-An, additional, Maples, Phillip B, additional, Chen, Salina, additional, Pappen, Beena, additional, Burke, James, additional, Ichimaru, Daiju, additional, Urata, Yasuo, additional, and Fujiwara, Toshiyoshi, additional

Published
2010
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210. Telomerase-Specific Virotheranostics for Human Head and Neck Cancer

Author

Kurihara, Yuji, primary, Watanabe, Yuichi, additional, Onimatsu, Hideki, additional, Kojima, Toru, additional, Shirota, Tatsuo, additional, Hatori, Masashi, additional, Liu, Dong, additional, Kyo, Satoru, additional, Mizuguchi, Hiroyuki, additional, Urata, Yasuo, additional, Shintani, Satoru, additional, and Fujiwara, Toshiyoshi, additional

Published
2009
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212. A Novel Antiangiogenic Effect for Telomerase-Specific Virotherapy through Host Immune System

Author

Ikeda, Yoshihiro, primary, Kojima, Toru, additional, Kuroda, Shinji, additional, Endo, Yoshikatsu, additional, Sakai, Ryo, additional, Hioki, Masayoshi, additional, Kishimoto, Hiroyuki, additional, Uno, Futoshi, additional, Kagawa, Shunsuke, additional, Watanabe, Yuichi, additional, Hashimoto, Yuuri, additional, Urata, Yasuo, additional, Tanaka, Noriaki, additional, and Fujiwara, Toshiyoshi, additional

Published
2009
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215. Okayama Igakkai Zasshi (Journal of Okayama Medical Association)

Author

Endo, Yoshikatsu, primary, Sakai, Ryo, additional, Ouchi, Masaaki, additional, Onimatsu, Hideki, additional, Hioki, Masayoshi, additional, Kagawa, Shunsuke, additional, Uno, Futoshi, additional, Watanabe, Yuichi, additional, Urata, Yasuo, additional, Tanaka, Noriaki, additional, and Fujiwara, Toshiyoshi, additional

Published
2008
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216. Okayama Igakkai Zasshi (Journal of Okayama Medical Association)

Author

Kishimoto, Hiroyuki, primary, Kojima, Toru, additional, Watanabe, Yuichi, additional, Kagawa, Shunsuke, additional, Fujiwara, Toshiya, additional, Uno, Futoshi, additional, Teraishi, Fuminori, additional, Kyo, Satoru, additional, Mizuguchi, Hiroyuki, additional, Hashimoto, Yuuri, additional, Urata, Yasuo, additional, Tanaka, Noriaki, additional, and Fujiwara, Toshiyoshi, additional

Published
2008
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219. Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model.

Author

Yano, Shuya, Takehara, Kiyoto, Miwa, Shinji, Kishimoto, Hiroyuki, Hiroshima, Yukihiko, Murakami, Takashi, Urata, Yasuo, Kagawa, Shunsuke, Bouvet, Michael, Fujiwara, Toshiyoshi, and Hoffman, Robert M.

Subjects
LIVER metastasis, COLON cancer treatment, GREEN fluorescent protein, TELOMERASE, ADENOVIRUSES, LIVER surgery, SURGICAL excision, HEALTH outcome assessment, THERAPEUTICS
Abstract

Fluorescence-guided surgery (FGS) of cancer is an area of intense development. In the present report, we demonstrate that the telomerase-dependent green fluorescent protein (GFP)-containing adenovirus OBP-401 could label colon-cancer liver metastasis in situ in an orthotopic mouse model enabling successful FGS. OBP-401-GFP-labeled liver metastasis resulted in complete resection with FGS, in contrast, conventional bright-light surgery (BLS) did not result in complete resection of the metastasis. OBP-401-FGS reduced the recurrence rate and prolonged over-all survival compared with BLS. In conclusion, adenovirus OBP-401 is a powerful tool to label liver metastasis in situ with GFP which enables its complete resection, not possible with conventional BLS. [ABSTRACT FROM AUTHOR]

Published
2016
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221. In vivo imaging of lymph node metastasis with telomerase-specific replication-selective adenovirus

Author

Kishimoto, Hiroyuki, primary, Kojima, Toru, additional, Watanabe, Yuichi, additional, Kagawa, Shunsuke, additional, Fujiwara, Toshiya, additional, Uno, Futoshi, additional, Teraishi, Fuminori, additional, Kyo, Satoru, additional, Mizuguchi, Hiroyuki, additional, Hashimoto, Yuuri, additional, Urata, Yasuo, additional, Tanaka, Noriaki, additional, and Fujiwara, Toshiyoshi, additional

Published
2006
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222. Enhanced antitumor efficacy of telomerase‐selective oncolytic adenoviral agent OBP‐401 with docetaxel: Preclinical evaluation of chemovirotherapy

Author

Fujiwara, Toshiya, primary, Kagawa, Shunsuke, additional, Kishimoto, Hiroyuki, additional, Endo, Yoshikatsu, additional, Hioki, Masayoshi, additional, Ikeda, Yoshihiro, additional, Sakai, Ryo, additional, Urata, Yasuo, additional, Tanaka, Noriaki, additional, and Fujiwara, Toshiyoshi, additional

Published
2006
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223. Histone deacetylase inhibitor FR901228 enhances the antitumor effect of telomerase-specific replication-selective adenoviral agent OBP-301 in human lung cancer cells

Author

Watanabe, Takanori, primary, Hioki, Masayoshi, additional, Fujiwara, Toshiya, additional, Nishizaki, Masahiko, additional, Kagawa, Shunsuke, additional, Taki, Masaki, additional, Kishimoto, Hiroyuki, additional, Endo, Yoshikatsu, additional, Urata, Yasuo, additional, Tanaka, Noriaki, additional, and Fujiwara, Toshiyoshi, additional

Published
2006
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224. 639. In Vivo Imaging of Lymph Node Metastasis with Telomerase-Specific Replication-Competent Adenovirus Containing Green Fluorescent Protein Gene

Author

Kojima, Toru, primary, Kishimoto, Hiroyuki, additional, Watanabe, Yuichi, additional, Kagawa, Shunsuke, additional, Teraishi, Fuminori, additional, Hioki, Masayoshi, additional, Ikeda, Yoshihiro, additional, Sakai, Ryo, additional, Urata, Yasuo, additional, Tanaka, Noriaki, additional, and Fujiwara, Toshiyoshi, additional

Published
2006
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225. 645. Combination of Oncolytic Adenovirotherapy and Bax Gene Therapy Does Not Augments Antitumor Efficacy

Author

Hioki, Masayoshi, primary, Kagawa, Shunsuke, additional, Fujiwara, Toshiya, additional, Ikeda, Yoshihiro, additional, Kojima, Toru, additional, Sakai, Ryo, additional, Uno, Futoshi, additional, Teraishi, Fuminori, additional, Shirakiya, Yoshiko, additional, Watanabe, Yuichi, additional, Hashimoto, Yuuri, additional, and Urata, Yasuo, additional

Published
2006
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228. 298. Combination Effect of Telomerase-Specific Replication-Competent Adenovirus (Telomelysin, OBP-301) and p53 Gene Therapy in Human Non- Small Cell Lung and Colon Cancer Cells

Author

Sakai, Ryo, primary, Teraishi, Fuminori, additional, Kojima, Toru, additional, Ikeda, Yoshihiro, additional, Hioki, Masayoshi, additional, Fujiwara, Toshiya, additional, Uno, Futoshi, additional, Kagawa, Shunsuke, additional, Hashimoto, Yuuri, additional, Watanabe, Yuichi, additional, Urata, Yasuo, additional, Tanaka, Noriaki, additional, and Fujiwara, Toshiyoshi, additional

Published
2006
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231. Visualization of Intrathoracically Disseminated Solid Tumors in Mice with Optical Imaging by Telomerase-Specific Amplification of a Transferred Green Fluorescent Protein Gene

Author

Umeoka, Tatsuo, primary, Kawashima, Takeshi, additional, Kagawa, Shunsuke, additional, Teraishi, Fuminori, additional, Taki, Masaki, additional, Nishizaki, Masahiko, additional, Kyo, Satoru, additional, Nagai, Katsuyuki, additional, Urata, Yasuo, additional, Tanaka, Noriaki, additional, and Fujiwara, Toshiyoshi, additional

Published
2004
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234. Telomerase-specific oncolytic adenovirus: Antitumor effects on radiation-resistant head and neck squamous cell carcinoma cells.

Author

Takahashi, Hideaki, Hyakusoku, Hiroshi, Horii, Chihiro, Takahashi, Masahiro, Nishimura, Goshi, Taguchi, Takahide, Kondo, Norio, Sakakibara, Atsuko, Urata, Yasuo, and Sano, Daisuke

Subjects
TELOMERASE, ADENOVIRUS diseases, HEAD & neck cancer, SQUAMOUS cell carcinoma, APOPTOSIS, DNA repair, GENE expression
Abstract

Background Radioresistance remains a critical issue in the use of radiotherapy for the treatment of head and neck squamous cell carcinoma (HNSCC). This study evaluated the efficacy of combination treatment with OBP-301, a telomerase-specific replication-selective adenovirus, and radiotherapy in overcoming radioresistance by examining its effect on radiation-resistant HNSCC cells. Methods Radiation-resistant HNSCC cells were treated with OBP-301 and radiation in vitro and in an orthotopic nude mouse model in vivo and synergism was assessed. Apoptosis and expression of MRN complex, which plays a key role in DNA repair machinery, were also analyzed. Results Infection with OBP-301 was found to enhance the antitumor efficacy of radiation both in vitro and in vivo by inhibiting MRN complex expression and increasing apoptosis induction. Conclusion Combined OBP-301 and radiation therapy seems to overcome radioresistance in HNSCC cells by inhibiting DNA repair machinery, and may thus be a novel therapeutic strategy for treating HNSCC. © 2013 Wiley Periodicals, Inc. Head Neck 36: 411-418, 2014 [ABSTRACT FROM AUTHOR]

Published
2014
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236. Safety and dose escalation of the targeted oncolytic adenovirus OBP-301 for refractory advanced liver cancer: Phase I clinical trial

Author

Heo, Jeong, Liang, Ja-Der, Kim, Chang Won, Woo, Hyun Young, Shih, I-Lun, Su, Tung-Hung, Lin, Zhong-Zhe, Yoo, So Young, Chang, Stanley, Urata, Yasuo, and Chen, Pei-Jer

Abstract

OBP-301 is an oncolytic adenovirus modified to replicate within cancer cells and lyse them. This open-label, non-comparative, phase I dose-escalation trial aimed to assess its safety and optimal dosage in 20 patients with advanced hepatocellular carcinoma. Good tolerance was shown with a maximum tolerated dose of 6 × 1012viral particles. The most common treatment-emergent adverse events were influenza-like illness, pyrexia, fatigue, decreased platelet count, abdominal distension, and anemia. Cohorts 4 and 5 had approximately 50% higher levels of CD8+ T cells in the peripheral blood after injection. The best target response occurred in 14 patients, 4 of whom had progressive disease. Multiple intratumoral injections of OBP-301 were well tolerated in patients with advanced hepatocellular carcinoma. The stable disease rate for the injected tumors was greater than the overall response rate, even with no obvious tumor response. OBP-301 might have a greater impact on local response as histological examination revealed that the presence of OBP-301 was consistent with the necrotic area at the injection site. Increased infiltration of CD8+ T cells and <1% PD-L1 expression were observed in tumors after injection. Improved antitumor efficacy might be achieved in future studies via viral injection with volume adjustment and in combination with other immuno-therapeutics.

Published
2023
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244. Telomelysin shows potent antitumor activity through apoptotic and non-apoptotic cell death in soft tissue sarcoma cells.

Author

Li, Gui‐Dong, Kawashima, Hiroyuki, Ogose, Akira, Ariizumi, Takashi, Hotta, Tetsuo, Kuwano, Ryozo, Urata, Yasuo, Fujiwara, Toshiyoshi, and Endo, Naoto

Abstract

This study investigated the pathway underlying the antitumor activity of telomelysin, a telomerase-dependent, replication-selective oncolytic adenovirus, in soft tissue sarcoma cells. Treatment with telomelysin alone resulted in simultaneous induction of apoptosis and autophagy, whereas cotreatment with telomelysin and 3-methyladenine significantly reduced cell viability and increased apoptosis and the cellular ATP level compared to treatment with telomelysin alone, indicating that telomelysin-mediated autophagy is a death-protective but not death-promoting process. Cotreatment with Z-Val-Ala-Asp- CH2F significantly increased cellular ATP depletion compared to telomelysin-alone treatment while inhibiting telomelysin-induced apoptosis and having no significant effect on cell viability, indicating that it promotes transition from apoptotic to necrotic cell death. [ABSTRACT FROM AUTHOR]

Published
2013
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246. Genetically engineered oncolytic adenovirus induces autophagic cell death through an E2F1- microRNA-7-epidermal growth factor receptor axis.

Author

Tazawa, Hiroshi, Yano, Shuya, Yoshida, Ryosuke, Yamasaki, Yasumoto, Sasaki, Tsuyoshi, Hashimoto, Yuuri, Kuroda, Shinji, Ouchi, Masaaki, Onishi, Teppei, Uno, Futoshi, Kagawa, Shunsuke, Urata, Yasuo, and Fujiwara, Toshiyoshi

Abstract

Autophagy is known to have a cytoprotective role under various cellular stresses; however, it also results in robust cell death as an important safeguard mechanism that protects the organism against invading pathogens and unwanted cancer cells. Autophagy is regulated by cell signalling including microRNA (miRNA), a post-transcriptional regulator of gene expression. Here, we show that genetically engineered telomerase-specific oncolytic adenovirus induced miR-7 expression, which is significantly associated with its cytopathic activity in human cancer cells. Virus-mediated miR-7 upregulation depended on enhanced expression of the E2F1 protein. Ectopic expression of miR-7 suppressed cell viability and induced autophagy by inhibiting epidermal growth factor receptor (EGFR) expression. Our results suggest that oncolytic adenovirus induces autophagic cell death through an E2F1- miR-7-EGFR pathway in human cancer cells, providing a novel insight into the molecular mechanism of an anticancer virotherapy. [ABSTRACT FROM AUTHOR]

Published
2012
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247. Efficient virotherapy for osteosarcoma by telomerase-specific oncolytic adenovirus.

Author

Li, Guidong, Kawashima, Hiroyuki, Ogose, Akira, Ariizumi, Takashi, Xu, Yongjun, Hotta, Tetsuo, Urata, Yasuo, Fujiwara, Toshiyoshi, and Endo, Naoto

Subjects
OSTEOSARCOMA, TELOMERASE, ADENOVIRUSES, CANCER cells, CELL lines, LABORATORY mice, BIOTECHNOLOGY, APOPTOSIS, XENOGRAFTS, THERAPEUTICS
Abstract

Purpose: A telomerase-specific oncolytic adenovirus, Telomelysin, can selectively kill cancer cells, and be attenuated in normal cells. We herein describe the oncolytic effect of Telomelysin on human osteosarcoma both in vitro and in vivo. Methods: The anti-tumor effects of Telomelysin were evaluated on human osteosarcoma cell lines in vitro and in a mouse xenograft model of human osteosarcoma in vivo. The replication efficiencies of Telomelysin in human osteosarcoma cell lines and normal cell lines and in osteosarcoma xenografts were determined by the expression levels of E1 mRNA and E1A protein using real-time quantitative PCR, Western blot analysis and immunohistochemistry. The in vitro telomerase-specific replication and the viral infection rate were also confirmed by TelomeScan (Telomelysin-GFP), using fluorescent microscopy and flow cytometry, respectively. The cell viabilities were examined by XTT assay, and the tumor volumes were measured every 2 days. The induction of apoptosis was assessed by Western blot analysis, as well as by TUNEL assay. Results: TelomeScan and Telomelysin were efficiently replicated in human osteosarcoma cell lines and led to a dose- and time-dependent expression of GFP, E1 mRNA and E1A protein. Telomelysin infection induced marked cytolysis and apoptosis in osteosarcoma cell lines in vitro. Neither cytotoxicity nor apoptosis were induced in normal human cell lines. In the human osteosarcoma cell xenograft model, intratumoral injection of Telomelysin resulted in increased viral replication, significant tumor growth suppression and distinct apoptotic cell death. Conclusions: This study indicated that virotherapy with Telomelysin may provide a promising strategy for the treatment of human osteosarcoma. [ABSTRACT FROM AUTHOR]

Published
2011
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249. Dendritic cell maturation is induced by p53-armed oncolytic adenovirus via tumor-derived exosomes enhancing systemic antitumor immunity.

Author

Ohtani, Tomoko, Kuroda, Shinji, Kanaya, Nobuhiko, Kakiuchi, Yoshihiko, Kumon, Kento, Hashimoto, Masashi, Yagi, Chiaki, Sugimoto, Ryoma, Kikuchi, Satoru, Kagawa, Shunsuke, Tazawa, Hiroshi, Urata, Yasuo, and Fujiwara, Toshiyoshi

Abstract

Dendritic cells (DCs) are crucial in cancer immunity, because they activate cytotoxic T cells by presenting tumor antigens. Recently, oncolytic virus therapy has been recognized as a systemic immune stimulator. We previously developed a telomerase-specific oncolytic adenovirus (OBP-301) and a p53-armed OBP-301 (OBP-702), demonstrating that these viruses strongly activate systemic antitumor immunity. However, their effects on DCs remained unclear. In the present study, the aim was to elucidate the mechanisms of DC activation by OBP-702, focusing particularly on tumor-derived exosomes. Exosomes (Exo53, Exo301, or Exo702) were isolated from conditioned media of human or murine pancreatic cancer cell lines (Panc-1, MiaPaCa-2, and PAN02) after treatment with Ad-p53, OBP-301, or OBP-702. Exo702 derived from Panc-1 and MiaPaCa-2 cells significantly upregulated CD86, CD80, CD83 (markers of DC maturation), and IFN-γ in DCs in vitro. Similarly, Exo702 derived from PAN02 cells upregulated CD86 and IFN-γ in bone marrow-derived DCs in a bilateral PAN02 subcutaneous tumor model. This DC maturation was inhibited by GW4869, an inhibitor of exosome release, and anti-CD63, an antibody targeting the exosome marker. Intratumoral injection of OBP-702 into PAN02 subcutaneous tumors significantly increased the presence of mature DCs and CD8-positive T cells in draining lymph nodes, leading to long-lasting antitumor effects through the durable activation of systemic antitumor immunity. In conclusion, tumor-derived exosomes play a significant role in DC maturation following OBP-702 treatment and are critical for the systemic activation of antitumor immunity, leading to the abscopal effect. [ABSTRACT FROM AUTHOR]

Published
2025
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