Search

Brain AVMs are rare but serious vascular lesions that often pose a management dilemma between the risk of various treatment modalities and uncertain natural history during observation. We describe preliminary data on the use of focused ultrasound as a novel therapeutic strategy. In an AVM model, one session of ultrasound gradually reduced flow through the lesion without inducing rupture. Due to its non-invasive yet immediate ablative effects, focused ultrasound may allow safer treatment of AVMs. However, further studies are needed to clarify its efficacy and side effect profile.

We present a transmission spectrum for the warm (500-600 K) sub-Neptune HD 3167c obtained using the Hubble Space Telescope Wide Field Camera 3 infrared spectrograph. We combine these data, which span the 1.125-1.643 μm wavelength range, with broadband transit measurements made using Kepler/K2 (0.6-0.9 μm) and Spitzer/IRAC (4-5 μm). We find evidence for absorption by at least one of H2O, HCN, CO2, and CH4 (Bayes factor 7.4; 2.5σ significance), although the data precision does not allow us to unambiguously discriminate between these molecules. The transmission spectrum rules out cloud-free hydrogen-dominated atmospheres with metallicities ≤100 solar at >5.8σ confidence. In contrast, good agreement with the data is obtained for cloud-free models assuming metallicities >700 solar. However, for retrieval analyses that include the effect of clouds, a much broader range of metallicities (including subsolar) is consistent with the data, due to the degeneracy with cloud-top pressure. Self-consistent chemistry models that account for photochemistry and vertical mixing are presented for the atmosphere of HD 3167c. The predictions of these models are broadly consistent with our abundance constraints, although this is primarily due to the large uncertainties on the latter. Interior structure models suggest that the core mass fraction is >40%, independent of a rock or water core composition, and independent of atmospheric envelope metallicity up to 1000 solar. We also report abundance measurements for 15 elements in the host star, showing that it has a very nearly solar composition.

Finding and exploiting loopholes is a familiar facet of fable, law, and everyday life. But cognitive, computational, and empirical work on this behavior remains scarce. Engaging with loopholes requires a nuanced understanding of goals, social ambiguity, and value alignment. We trace loophole behavior to early childhood, and we propose that exploiting loopholes results from a conflict in actors' goals combined with a pressure to cooperate. A survey of 260 parents reporting on 425 children reveals that loophole behavior is prevalent, frequent, and diverse in daily parent-child interactions, emerging around ages five to six and tapering off from around ages nine to ten into adolescence. A further experiment shows that adults consider loophole behavior in children as less costly than non-compliance, and children increasingly differentiate loophole behavior from non-compliance from ages four to ten. We discuss limitations of the current work together with a proposal for a formal framework for loophole behavior.

While educators, curriculum authors and policy makers alike are influenced by assumptions about parents' dis/approval of gender and sexuality diversity, both generally, as well as specifically in relation to this topic's appropriateness for K-12 classrooms, little empirical data is available to support these assumptions. What data does exist suggests that parents generally support the implementation of a comprehensive sexuality curriculum, inclusive of same-sex attraction, and view sexuality education as a school-parent partnership. Surveying a sample of Australian parents of children attending a government (public) school (N = 2093), whose responses were weighted to produce nationally representative estimates, this study sought to expand on previous findings by exploring the complexities of parents' attitudes in relation to gender and sexuality diversity and its place within relationships and sexual health education. This paper provides a descriptive overview of parents' ideas about the purpose of relationships and sexual health education and their views on the importance of including gender and sexuality diversity-inclusive content within related curriculum areas. Notably, over 80% of parents supported the inclusion of gender and sexuality diversity-inclusive relationships and sexual health education topics across primary and secondary government schools.

Objective: Examine uptake (e.g., initial session participation) and retention (e.g., booster session participation) in an intervention about responding to sexual assault and partner abuse disclosures. Participants: Participants were 836 students (primarily White; upper-middle class) at a medium-sized university. Method: Participants completed baseline surveys, were invited to a two-session intervention, and responded to a follow-up survey. Results: Initial session attendance was 36.2% (n = 303); of those, 83.1% (n = 252) attended the booster. Female, sexual minority students, and students with fewer prior negative reactions, and higher initial session satisfaction were more likely to attend than other students. Participants' reported reasons for not attending included scheduling problems and topic discomfort. Participants reported that remote attendance and higher cash incentives would have made attendance more likely. Conclusion: Findings indicate the draw of cash incentives, a need to reach high-risk students and integrate into existing organizations, and the potential for individualized prevention.

An amendment to this paper has been published and can be accessed via the original article.

BackgroundThe western flower thrips, Frankliniella occidentalis (Pergande), is a globally invasive pest and plant virus vector on a wide array of food, fiber, and ornamental crops. The underlying genetic mechanisms of the processes governing thrips pest and vector biology, feeding behaviors, ecology, and insecticide resistance are largely unknown. To address this gap, we present the F. occidentalis draft genome assembly and official gene set.ResultsWe report on the first genome sequence for any member of the insect order Thysanoptera. Benchmarking Universal Single-Copy Ortholog (BUSCO) assessments of the genome assembly (size = 415.8 Mb, scaffold N50 = 948.9 kb) revealed a relatively complete and well-annotated assembly in comparison to other insect genomes. The genome is unusually GC-rich (50%) compared to other insect genomes to date. The official gene set (OGS v1.0) contains 16,859 genes, of which ~ 10% were manually verified and corrected by our consortium. We focused on manual annotation, phylogenetic, and expression evidence analyses for gene sets centered on primary themes in the life histories and activities of plant-colonizing insects. Highlights include the following: (1) divergent clades and large expansions in genes associated with environmental sensing (chemosensory receptors) and detoxification (CYP4, CYP6, and CCE enzymes) of substances encountered in agricultural environments; (2) a comprehensive set of salivary gland genes supported by enriched expression; (3) apparent absence of members of the IMD innate immune defense pathway; and (4) developmental- and sex-specific expression analyses of genes associated with progression from larvae to adulthood through neometaboly, a distinct form of maturation differing from either incomplete or complete metamorphosis in the Insecta.ConclusionsAnalysis of the F. occidentalis genome offers insights into the polyphagous behavior of this insect pest that finds, colonizes, and survives on a widely diverse array of plants. The genomic resources presented here enable a more complete analysis of insect evolution and biology, providing a missing taxon for contemporary insect genomics-based analyses. Our study also offers a genomic benchmark for molecular and evolutionary investigations of other Thysanoptera species.

Objetivo: analisar as vivências do diagnóstico de Doença Renal Crônica sob a ótica dos pacientes. Método: estudo qualitativo realizado por meio de entrevistas semiestruturadas realizadas junto aos pacientes atendidos em um serviço de hemodiálise; os dados foram submetidos à análise de Conteúdo de Bardin. Resultados: as vivências foram organizadas nas seguintes categorias significativas: Sentimentos vivenciados no momento do diagnóstico; Impactos do diagnóstico; Negação da doença; O paradoxo da gratidão e Enfrentamento da doença. Considerações finais: a vivência do diagnóstico da Doença Renal Crônica adquire uma perspectiva singular para cada paciente subsidiada por aspectos de ordem subjetiva, pelo suporte assistencial e familiar ao qual ele tem acesso.

Environmental and climatic changes in northern Europe have shaped a geographical area in which new tick species may become established and introduce new tick-borne pathogens. In recent decades, ticks have expanded their latitudinal and altitudinal range limits in northern Sweden. In this study, ticks were collected in 2018 and 2019 in northern Sweden from different hosts, mainly from dogs, cats and humans. The ticks in 2018 (n = 2141, collected from 65 municipalities in 11 provinces) were identified as Ixodes ricinus (n = 2108, 98.5%), Ixodes persulcatus (n = 18, 0.8%), Ixodes trianguliceps (n = 14, 0.7%) and Hyalomma marginatum (n = 1, 0.05%). The ticks collected in 2019 (n = 519, across a smaller area than in 2018, i.e. Sweden's four northernmost provinces) were identified as I. ricinus (n = 242, 46.6%) and I. persulcatus (n = 277, 53.4%). Among those collected in 2019, the majority of I. ricinus (n = 111, 45.9%) were submitted from the province of Västerbotten, while most I. persulcatus (n = 259, 93.5%) were collected in the province of Norrbotten. This study provides updated figures on the geographical distribution of two Ixodes species in northern Sweden. The results confirmed I. ricinus to be the dominant species and that I. persulcatus has enlarged its distributional area compared with previous reports. Updated knowledge of tick distribution is fundamental for the creation of risk maps and will allow relevant advice to be provided to the general public, suggesting measures to prevent tick bites and consequently tick-borne diseases.

The Endosomal Sorting Complexes Required for Transport (ESCRT) machinery mediates the membrane fission step that completes cytokinetic abscission and separates dividing cells. Filaments composed of ESCRT-III subunits constrict membranes of the intercellular bridge midbody to the abscission point. These filaments also bind and recruit cofactors whose activities help execute abscission and/or delay abscission timing in response to mitotic errors via the NoCut/Abscission checkpoint. We previously showed that the ESCRT-III subunit IST1 binds the cysteine protease Calpain-7 (CAPN7) and that CAPN7 is required for both efficient abscission and NoCut checkpoint maintenance (Wenzel et al., 2022). Here, we report biochemical and crystallographic studies showing that the tandem microtubule-interacting and trafficking (MIT) domains of CAPN7 bind simultaneously to two distinct IST1 MIT interaction motifs. Structure-guided point mutations in either CAPN7 MIT domain disrupted IST1 binding in vitro and in cells, and depletion/rescue experiments showed that the CAPN7-IST1 interaction is required for (1) CAPN7 recruitment to midbodies, (2) efficient abscission, and (3) NoCut checkpoint arrest. CAPN7 proteolytic activity is also required for abscission and checkpoint maintenance. Hence, IST1 recruits CAPN7 to midbodies, where its proteolytic activity is required to regulate and complete abscission.

The global distribution of footpaths and their inferred antiquity implies that they are widespread spatial and temporal anthropogenic landscape units. Arid environments are of special interest for investigating historically used footpaths, as older routes may preserve better due to minimal modern impact and slower pedogenic processes. Here we examine footpaths in the Judean Desert of the southern Levant, a human hotspot throughout the Holocene. We studied one modern and two archaeological footpaths (one attributed to the Early Bronze Age and one to the Roman period) using micromorphology, bulk samples laboratory analysis, and remote sensing. Field observations and color analysis indicate that footpaths in the studied arid limestone environment can result in brighter surface color than their non-path surroundings. Similar color changes are reflected using both laboratory analysis and high-resolution remote sensing, where the difference is also significant. Microscopically, the footpaths studied tend to be less porous and with fewer biogenic activities when compared to their non-path controls. However, the two ancient footpaths studied do exhibit minimal indicators of biogenic activities that are not detectable in the modern footpath sample. Our study shows that high-resolution remote sensing coupled with micromorphology, while using appropriate local modern analogies, can help to locate and assess both the environmental effect and the antiquity of footpaths.

The advent of data-based modeling has provided new methods and algorithms for analyzing the complex flow fields in high-speed combustion applications. These techniques can be used to study the fluid dynamics and reaction progress in different regions of the flow, which can provide insight into the underlying physics of the system while helping one identify avenues for the development and application of new models. The present work implements two such algorithms – the standard K-means clustering algorithm and a modified Jacobian-scaled K-means clustering algorithm – to analyze a high-speed reacting flow field. The results show that the Jacobian-scaled K-means algorithm allocates more clusters in the regions of the thermochemical state space where chemical source term is highest. In physical space, this concentrates the flow partitions in regions where heat release rate is higher and chemical timescales are lower. This is observed for different cluster numbers and data sets. When moving between data sets that occupy different portions of the state space, the cluster centroid locations are found to be less sensitive to the data set when the Jacobian-scaled K-means algorithm is used. Altogether, the modified K-means algorithm provides a new tool for identifying thermodynamic and thermochemical similarities in and across compressible reactive flow fields.

Tomato spotted wilt virus (TSWV; species Tomato spotted wilt orthotospovirus; genus Orthotospovirus; family Tospoviridae) is a thrips-transmitted virus that can cause substantial economic losses to many crops, including tomato (Solanum lycopersicum). Since 2005, TSWV emerged as an economically important virus of processing tomatoes in the Central Valley of California, in part due to increased populations of the primary thrips vector, western flower thrips (WFT; Frankliniella occidentalis). To develop an understanding of the epidemiology of TSWV in this region, population densities of WFT and incidence of TSWV were monitored in California's processing tomato transplant-producing greenhouses and associated open fields from 2007 to 2013. Thrips were monitored with yellow sticky cards and in tomato flowers, whereas TSWV incidence was assessed with indicator plants and field surveys for virus symptoms. All thrips identified from processing tomato fields were WFT, and females were three-fold more abundant on sticky cards than males. Symptoms of TSWV infection were observed in all monitored processing tomato fields. Incidences of TSWV ranged from 1 to 20%, with highest incidence found in late-planted fields. There was no single primary inoculum source, and inoculum sources for thrips/TSWV varied depending on the production region. These results allowed us to develop a model for TSWV infection of processing tomatoes in the Central Valley of California. The model predicts that low levels of primary TSWV inoculum are amplified in early-planted tomatoes and other susceptible crops leading to highest levels of infection in later-planted fields, especially those with high thrips populations. Based upon these findings, an integrated pest management (IPM) strategy for TSWV in processing tomatoes in California was devised. This IPM strategy focuses on strategic field placement (identification of high-risk situations), planting TSWV- and thrips-free transplants, planting resistant varieties, monitoring for TSWV symptoms and thrips, roguing infected plants, thrips management targeting early generations, extensive sanitation after harvest, and strategic cropping to avoid overlap with winter bridge crops.

During cell division, remodelling of the nuclear envelope enables chromosome segregation by the mitotic spindle1. The reformation of sealed nuclei requires ESCRTs (endosomal sorting complexes required for transport) and LEM2, a transmembrane ESCRT adaptor2-4. Here we show how the ability of LEM2 to condense on microtubules governs the activation of ESCRTs and coordinated spindle disassembly. The LEM motif of LEM2 binds BAF, conferring on LEM2 an affinity for chromatin5,6, while an adjacent low-complexity domain (LCD) promotes LEM2 phase separation. A proline-arginine-rich sequence within the LCD binds to microtubules and targets condensation of LEM2 to spindle microtubules that traverse the nascent nuclear envelope. Furthermore, the winged-helix domain of LEM2 activates the ESCRT-II/ESCRT-III hybrid protein CHMP7 to form co-oligomeric rings. Disruption of these events in human cells prevented the recruitment of downstream ESCRTs, compromised spindle disassembly, and led to defects in nuclear integrity and DNA damage. We propose that during nuclear reassembly LEM2 condenses into a liquid-like phase and coassembles with CHMP7 to form a macromolecular O-ring seal at the confluence between membranes, chromatin and the spindle. The properties of LEM2 described here, and the homologous architectures of related inner nuclear membrane proteins7,8, suggest that phase separation may contribute to other critical envelope functions, including interphase repair8-13 and chromatin organization14-17.

BackgroundCurrent guidelines for the treatment of adult severe traumatic brain injury (sTBI) consist of high-quality evidence reports, but they are no longer accompanied by management protocols, as these require expert opinion to bridge the gap between published evidence and patient care. We aimed to establish a modern sTBI protocol for adult patients with both intracranial pressure (ICP) and brain oxygen monitors in place.MethodsOur consensus working group consisted of 42 experienced and actively practicing sTBI opinion leaders from six continents. Having previously established a protocol for the treatment of patients with ICP monitoring alone, we addressed patients who have a brain oxygen monitor in addition to an ICP monitor. The management protocols were developed through a Delphi-method-based consensus approach and were finalized at an in-person meeting.ResultsWe established three distinct treatment protocols, each with three tiers whereby higher tiers involve therapies with higher risk. One protocol addresses the management of ICP elevation when brain oxygenation is normal. A second addresses management of brain hypoxia with normal ICP. The third protocol addresses the situation when both intracranial hypertension and brain hypoxia are present. The panel considered issues pertaining to blood transfusion and ventilator management when designing the different algorithms.ConclusionsThese protocols are intended to assist clinicians in the management of patients with both ICP and brain oxygen monitors but they do not reflect either a standard-of-care or a substitute for thoughtful individualized management. These protocols should be used in conjunction with recommendations for basic care, management of critical neuroworsening and weaning treatment recently published in conjunction with the Seattle International Brain Injury Consensus Conference.

When we reason about the goals of others, how do we balance the positive outcomes that actions led to, with the potentiallybad ways those actions could have ended? In a four-part experiment, we tested whether and how adults (full study) and6- to 8-year-old children (ongoing study) expect other agents to take account of the ways their goal-directed action couldhave failed. Across 4 different tasks, we found that adults expected others to negatively appraise perilous situations (deeptrenches), to minimize the danger of their actions, and to trade off danger and reward in their action plans. Our preliminarychildrens study shows similar trends. These results suggest that people appeal to peril-how badly things could go if onesactions fail-when explaining and predicting other peoples actions, and also make quantitative inferences that are finelytuned to the degree of peril and reward that others face.

People make fast, spontaneous, and consistent judgementsof social situations, even in complex physical contexts withmultiple-body dynamics (e.g. pushing, lifting, carrying, etc.).What mental computations make such judgments possible? Dopeople rely on low-level perceptual cues, or on abstract con-cepts of agency, action, and force? We describe a new exper-imental paradigm, Flatland, for studying social inference inphysical environments, using automatically generated interac-tive scenarios. We show that human interpretations of events inFlatland can be explained by a computational model that com-bines inverse hierarchical planning with a physical simulationengine to reason about objects and agents. This model out-performs cue-based alternatives based on hand-coded (multi-nomial logistic regression) and learned (LSTM) features. Ourresults suggest that humans could use a combination of intu-itive physics and hierarchical planning to interpret complex in-teractive scenarios encountered in daily life.

We are surprised when events violate our intuitive physicalexpectations. Even infants look longer when things seem tomagically teleport or vanish. This important surprise signalhas been used to probe what infants expect, in order to studythe most basic representations of objects. But these studiesrely on binary measures – an event is surprising, or not. Here,we study surprise in a more precise, quantitative way, usingthree distinct measures: we ask adults to judge how surprisinga scene is, when that scene is surprising, and why it is surpris-ing. We find good consistency in the level of surprise reportedacross these experiments, but also crucial differences in theimplied explanations of those scenes. Beyond this, we showthat the timing and degree of surprise can be explained by anobject-based model of intuitive physics.

Abstract Background The composition of the microbial flora associated with ixodid ticks has been studied in several species, revealing the importance of geographical origin, developmental stage(s) and feeding status of the tick, as well as substantial differences between tissues and organs. Studying the microbiome in the correct context and scale is therefore necessary for understanding the interactions between tick-borne pathogens and other microorganisms as well as other aspects of tick biology. Methods In the present study the microbial flora of whole Ixodes ricinus, I. persulcatus and I. trianguliceps ticks were analyzed with 16S rRNA amplicon sequencing. Additionally, tick organs (midguts, Malpighian tubules, ovaries, salivary glands) from flat and engorged I. ricinus female ticks were examined with the same methodology. Results The most abundant bacteria belonged to the group of Proteobacteria (Cand. Midichloria mitochondrii and Cand. Lariskella). 16S amplicon sequencing of dissected tick organs provided more information on the diversity of I. ricinus-associated microbial flora, especially when organs were collected from engorged ticks. Bacterial genera significantly associated with tick feeding status as well as genera associated with the presence of tick-borne pathogens were identified. Conclusions These results contribute to the knowledge of microbial flora associated with ixodid ticks in their northernmost distribution limit in Europe and opens new perspectives for other investigations on the function of these bacteria, including those using other approaches like in vitro cultivation and in vitro models. Graphical Abstract

Various natural products used for water treatment are becoming more popular due to their general safety, eco-friendly sludge production, ease of degradation, cost-effectiveness, and local availability. This research assessed the efficiency of powdered neem (Azadirachta indica) seeds in removing turbidity from the water. Batch experiments determined the optimum coagulant dose, pH level, mixing time, and mixing speed to reduce turbidity from kaolin-based synthetic turbid water. Powdered neem seeds with a pore size of about 0.45 mm were prepared and used in water treatment under optimum conditions. Results showed that a coagulant dose of 3 g of neem seeds/L, 13.2 pH level, 60 mins mixing time at 80 rpm mixing speed could reduce turbidity levels to 35 NTU from 250 NTU (86% removal). Findings suggest that powdered neem seeds can be a potential substitute for conventional chemical coagulants for drinking water treatment.

Predicting a workforce crisis for the past twenty years, the transportation field has launched a wide variety of initiatives to increase the future talent pipeline that would choose transportation as a field of choice for study and future careers. This project follows on the premise that increasing awareness of career opportunities is essential to attracting new entrants at an early age, and that awareness building should be dynamic. This includes providing views of who works in the transportation field, what their experiences are, and what they value about their work. This paper hypothesizes that first-person glimpses into transportation careers are just as essential for job seekers as knowing job specs, qualifications, pay scales and opportunities for advancement. In-depth career profiles of workers were developed and showcase individuals, from diverse backgrounds and interests, engaged in work critical to the future of our transportation systems and infrastructure as an inducement to consider future education and training work needed to enter such a field. A number of other initiatives have created occupation and career profiles in public and private agencies. As with other awareness building and talent pipeline development initiatives such efforts need to be made accessible to a wider more diverse audience and metrics for tracking their effectiveness should be developed and implemented. Coordinating and testing these efforts are part of a future stage of program.View the NCST Project Webpage

BackgroundManagement algorithms for adult severe traumatic brain injury (sTBI) were omitted in later editions of the Brain Trauma Foundation's sTBI Management Guidelines, as they were not evidence-based.MethodsWe used a Delphi-method-based consensus approach to address management of sTBI patients undergoing intracranial pressure (ICP) monitoring. Forty-two experienced, clinically active sTBI specialists from six continents comprised the panel. Eight surveys iterated queries and comments. An in-person meeting included whole- and small-group discussions and blinded voting. Consensus required 80% agreement. We developed heatmaps based on a traffic-light model where panelists' decision tendencies were the focus of recommendations.ResultsWe provide comprehensive algorithms for ICP-monitor-based adult sTBI management. Consensus established 18 interventions as fundamental and ten treatments not to be used. We provide a three-tier algorithm for treating elevated ICP. Treatments within a tier are considered empirically equivalent. Higher tiers involve higher risk therapies. Tiers 1, 2, and 3 include 10, 4, and 3 interventions, respectively. We include inter-tier considerations, and recommendations for critical neuroworsening to assist the recognition and treatment of declining patients. Novel elements include guidance for autoregulation-based ICP treatment based on MAP Challenge results, and two heatmaps to guide (1) ICP-monitor removal and (2) consideration of sedation holidays for neurological examination.ConclusionsOur modern and comprehensive sTBI-management protocol is designed to assist clinicians managing sTBI patients monitored with ICP-monitors alone. Consensus-based (class III evidence), it provides management recommendations based on combined expert opinion. It reflects neither a standard-of-care nor a substitute for thoughtful individualized management.

Humans routinely make inferences about both the contents and the workings of other minds based on observed actions. People consider what others want or know, but also how intelligent, rational, or attentive they might be. Here, we introduce a new methodology for quantitatively studying the mechanisms people use to attribute intelligence to others based on their behavior. We focus on two key judgments previously proposed in the literature: judgments based on observed outcomes (you're smart if you won the game) and judgments based on evaluating the quality of an agent's planning that led to their outcomes (you're smart if you made the right choice, even if you didn't succeed). We present a novel task, the maze search task (MST), in which participants rate the intelligence of agents searching a maze for a hidden goal. We model outcome-based attributions based on the observed utility of the agent upon achieving a goal, with higher utilities indicating higher intelligence, and model planning-based attributions by measuring the proximity of the observed actions to an ideal planner, such that agents who produce closer approximations of optimal plans are seen as more intelligent. We examine human attributions of intelligence in three experiments that use MST and find that participants used both outcome and planning as indicators of intelligence. However, observing the outcome was not necessary, and participants still made planning-based attributions of intelligence when the outcome was not observed. We also found that the weights individuals placed on plans and on outcome correlated with an individual's ability to engage in cognitive reflection. Our results suggest that people attribute intelligence based on plans given sufficient context and cognitive resources and rely on the outcome when computational resources or context are limited.