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While angiotensin II (Ang II) has been shown to inhibit migration of extravillous trophoblasts via plasminogen activator inhibitor-1 (PAI-1) activation, it has remained unclear whether it stimulates or inhibits malignant behavior of choriocarcinoma cells. Since we previously found an involvement of the renin-angiotensin system (RAS) in the proliferative potential in choriocarcinoma cells (BeWo), mediated via the Ang II type 1 receptor (AT1R), in the present study we investigated the effects of Ang II on choriocarcinoma cell migration/invasion in vitro using Transwell cell culture chambers. Ang II (10(-8)M) promoted migration and invasion by a choriocarcinoma cell line and augmented random cell mobility on checkerboard analysis. Immunoblotting showed Ang II to activate the phosphorylation of FAK and Akt in BeWo cells. Furthermore Ang II effects on cell migration were abolished by a selective AT1R antagonist and a phosphatidylinositol 3-kinase (PI3K) inhibitor. The present results suggest that Ang II-induced migration and invasion of choriocarcinoma cells probably involves PI3K following binding to the AT1R.

Oxytocin (OT) is known to cause vascular relaxation via nitric oxide (NO) production and proliferation of endothelial cells. Previously we revealed that human umbilical vascular endothelial cells (HUVECs) expresses placental leucine aminopeptidase (P-LAP)/insulin regulated aminopeptidase (IRAP), which becomes translocation to the cell surface on activation of oxytocin receptor (OTR). However, the physiological roles of P-LAP in HUVECs have not been elucidated.Actions of OT on HUVECs transfected with a copy of the human P-LAP gene were therefore examined with a focus on changes in parameters linked to OTR such as calcium mobilization, NO production and cell proliferation.Cell surface P-LAP activity was significantly elevated (approximately 3 fold) in P-LAP overexpressing-HUVECs and overexpression of P-LAP resulted in remarkable inhibition of OT effects on HUVECs such as cell proliferation, [Ca2+]i, and NO production.These findings suggested that P-LAP on the plasma membrane in HUVECs regulates the effects of OT with resolution around OTR.

s / Placenta 35 (2014) A1eA112 A50 MTS assay. (3) Migration assay, invasion assay, and adhesion assay were performed. Results: C2GnT was highly expressed in trophoblasts of choriocarcinoma but not in hydatidiform mole. (1) Expression level of C2GnT protein in siRNA transfectants were significantly reduced compared to WT and controls at 24 hours after transfection. (2) MTS assay showed no affection on cell proliferation in Jar after C2GnT siRNA transfection for 72 hours. (3) Suppression of C2GnT expression reduced migration and invasive abilities significantly. The rate of cell attachment to fibronectin, collagen type I, and collagen type IV were decreased by C2GnT knockdown. Conclusion: These findings suggest that C2GnT is involved in regulating invasion of choriocarcinoma and it might be through modifications of the oligosaccharide chains of hCG. P1.127-N. TRANSCRIPTIONAL REGULATION OF HUMAN NACETYLGLUCOSAMINYLTRANSFERASE IVA GENE IN TROPHOBLASTS Yoko Miwa , Eiko Yamamoto , Kanako Shinjo , Kaoru Niimi , Kimihiro Nishino , Yukio Mano , Seiji Sumigama , Tomomi Kotani , Fumitaka Kikkawa a Nagoya University, Nagoya City, Japan; b Toyota Kosei Hospital

s / Placenta 35 (2014) A1eA112 A65 and non-transmitting dams were compared. However, significant increases in TNFa protein (p < 0.05, t-test) were observed by ELISA in serum of CMV-transmitting dams (n1⁄43) at 21 dpi (mean 980 pg/ml), compared to non-transmitters (n1⁄43

Objective To investigate the protective effect of sphingosine-1-phosphate (S1P) against H 2 O 2 -induced apoptosis in human granulosa cell cultures with freshly harvested granulosa cells. Design Experimental study. Setting Academic medical center for reproductive medicine. Patient(s) Cultures of primary granulosa cells isolated from women undergoing in vitro fertilization (IVF). Intervention(s) None. Main Outcome Measure(s) Cell apoptosis and Western blot analysis of signaling pathway proteins. Result(s) We found that S1P (1 and 10 mM) statistically significantly decreased granulosa cell apoptosis after H 2 O 2 treatment. The decreased cell apoptosis induced by S1P was abolished after treatment with VPC23019, an inhibitor of S1P1 and S1P3 receptors, W146, an inhibitor of S1P1 receptors, and CAY10444, an inhibitor of S1P3 receptors. A Western blot analysis revealed that the level of phospho-Akt increased and peaked at 10 minutes after 10 mM S1P exposure. Conclusion(s) Treatment with S1P can inhibit the apoptosis of granulosa cells in response to oxidative stress induced by H 2 O 2 . The protective effect of S1P is mediated by activating the PI3K/Akt pathway, and the antiapoptotic effect of S1P is mainly mediated through the S1P1 and S1P3 receptor.

Objective To report the case of a patient with factor V deficiency who achieved pregnancy with the planned transfusion of fresh frozen plasma (FFP) while monitoring follicle development and ovulation induction using gonadotropin. Design Case report. Setting University hospital. Patient(s) A 28-year-old nulliparous female. Intervention(s) Medical management including infertility treatment. Main Outcome Measure(s) Clinical follow-up. Result(s) A patient with factor V deficiency experienced repeated ovulation-related hemoperitoneum following the withdrawal of oral contraceptive pills. The monitoring of follicle development and ovulation induction using gonadotropin followed by FFP transfusion was useful to avoid hemoperitoneum. Pregnancy was achieved within a relatively short period using intrauterine insemination. Conclusion(s) Planned prophylactic FFP administration and intervention with infertility treatment might be useful to minimize the risk of ovulation-related hemoperitoneum in patients with factor V deficiency.

normalized platelet number. IL-6 and IL-10 levels did not show differences between L-NAME and silibinin groups. Histopathologic lesions in liver of the L-NAME group showed loss of polyedric shape of hepatocyte and acinar architecture. Intense mononuclear inflammatory infiltrate and thickening of muscle tunica of arterial vessel were observed mainly in periportal area. Silibinin treatment produced significant attenuation of the periportal inflammatory infiltrate observed in the L-NAME group, showing an association between inflammatory infiltrate and TNF-alpha, IL-1 and IFN-gamma levels. Conclusion: The silibinin treatment in different periods of pregnancy showed that this flavonoid displays anti-inflammatory and immunomodulatory actions that may contribute to its hepatoprotective effects. FAPESP: Grant no. 2006/07095-3

The requirement of safe child delivery is increasing in developed countries in these decades. We have developed an obstetric forceps contouring the infant's head surface with stiffness-controlled pads. The stiffness of the pad is controlled by using air pressure regulation based on jamming transition. The proposed forceps has two soft material pads facing to the infant's head. The proposed forceps is first thin to get safely inserted into the delivery cavity. The granular tetrapod particles are then injected into the pads to contour the infant's head surface. By vacuuming the inside air of the pads, the particles interlock each other and the pads move onto the solid-like phase. In the experiments, the proposed method worked effectively to decrease the pressure onto the infant's head during delivery assistance and consequently made the operation safer.