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With the exception of chloroform, the rest of trihalomethanes (THM), the so-called brominated THM (Br-THM) are composed of bromine-substituted molecules with increased lipophilicity and potency to biological tissues. The THM are formed within disinfected tap water and their health effects, under research, range from cancer to adverse reproductive outcomes. However, the association between human exposures to Br-THM and the risk of developing type II diabetes mellitus (T2DM) and metabolic co-morbidities, such as obesity and non-alcoholic steatohepatitis has never been previously explored. The objective of this exploratory analysis was to address obesity-mediated associations between urinary concentrations of brominated THM and incidences of T2DM in a Cypriot adult population (n = 326). First morning urine voids were collected once during summer and another time during winter while a detailed questionnaire was administered to participants. Creatinine-adjusted urinary Br-THM analyte concentrations were significantly (p < 0.05) higher in T2DM cases when compared with those in healthy individuals. Multivariate logistic regression models adjusted for potential confounders showed that participants with ≥ 30 kg m− 2 BMI were at a higher T2DM risk (OR = 8.42, 95% CI: 1.97, 45.5; p < 0.01) when compared with that of normal weight participants ( < 25 kg m− 2). About 4 times higher risk for developing T2DM was observed for individuals in the upper tertile of urinary Br-THM levels (OR = 3.99, 95% CI: 1.07, 19.7; p < 0.05) when compared with the lower tertile participants. Among the participants with BMI ≥ 25 kg m− 2, urinary Br-THM levels were significantly (p < 0.001) higher in diabetics than in healthy individuals. Ingestion and non-ingestion exposures to Br-THM deserve careful consideration in relevant epidemiological studies, as a possible environmental risk factor of T2DM.

Water contamination and noise have been consistently the least assessed environmental/lifestyle exposures in pregnancy-birth cohorts (PBC). Water quality surveillance data collected during the past decade within urban drinking-water distribution systems call for re-evaluation of water and health issues in the developed world. The objectives of this scientific commentary were to (i) highlight the extent of appraisal of water contamination in exposure assessment studies of PBC, worldwide, and (ii) propose recommendations to increase awareness of emerging water-related risks through their improved representation into PBC study designs in urban centers. Three scientific literature databases (Scopus, PubMed, and Web of Science) were used for a systematic search on worldwide PBC and their publications that considered water contamination and health outcomes. Publicly-available e-databases (ENRIECO, BIRTHCOHORTS, and CHICOS) were also employed for detailed exploration of existing European Union (EU)-based PBC. Out of the 76 PBC identified in the EU territory, only 12 of them incorporated water contamination into their study designs. Among which only 6 PBC published scientific articles that either included data on water contamination and/or water intake estimates. Trihalomethanes but not other disinfection by-products were mostly studied in the PBC around the globe, while fluoride, atrazine, perfluorinated compounds, tetrachloroethylene, and lead were studied to a lesser extent as water contaminants. It appears that chemical-based water contamination and corresponding human exposures represent a largely underappreciated niche of exposure science pertaining to pregnant mother and children's health in PBC. Future PBC studies should grasp this opportunity to substantially reform elements of water contamination in their exposure assessment protocols and effectively combine them with their epidemiological study designs.

Previous epidemiological studies linking drinking water total trihalomethanes (THM) with pregnancy disorders or bladder cancer have not accounted for specific household cleaning activities that could enhance THM exposures. We examined the relation between household cleaning activities (washing dishes/clothes, mopping, toilet cleaning, and washing windows/surfaces) and urinary THM concentrations accounting for water sources, uses, and demographics. A cross-sectional study (n = 326) was conducted during the summer in Nicosia, Cyprus, linking household addresses to the geocoded public water pipe network, individual household tap water, and urinary THM measurements. Household tap water THM concentrations ranged between 3-129 μg L -1, while the median (Q1, Q3) creatinine-adjusted urinary THM concentration in females (669 ng g-1 (353, 1377)) was significantly (p < 0.001) higher than that in males (399 ng g-1, (256, 681)). Exposure assessment, based on THM exposure equivalency units, showed that hand dishwashing, mopping, and toilet cleaning significantly (p < 0.001) increased urinary THM levels. The effect of dishwashing by females ≥36 y of age remained significant, even after adjusting for potential confounders. No significant (p > 0.05) association was observed between ingestion-based THM exposure equivalency units and urinary THM. Noningestion routes of THM exposures during performance of routine household cleaning activities were shown for the first time to exert a major influence on urinary THM levels. It is warranted that future pregnancy-birth cohorts include monitoring of noningestion household THM exposures in their study design.

Changes in disinfectant type could trigger a cascade of reactions releasing pipe-anchored metals/metalloids into finished water. However, the effect of pre-formed disinfection by-products on the release of sorbed contaminants (arsenic-As in particular) from drinking water distribution system pipe scales remains unexplored. A bench-scale study using a factorial experimental design was performed to evaluate the independent and interaction effects of trihalomethanes (TTHM) and haloacetic acids (HAA) on arsenic (As) release from either scales-only or scale-biofilm conglomerates (SBC) both anchored on asbestos/cement pipe coupons. A model biofilm (Pseudomonas aeruginosa) was allowed to grow on select pipe coupons prior experimentation. Either TTHM or HAA individual dosing did not promote As release from either scales only or SBC, detecting < 6 μg As L− 1 in finished water. In the case of scales-only coupons, the combination of the highest spike level of TTHM and HAA significantly (p < 0.001) increased dissolved and total As concentrations to levels up to 16 and 95 μg L− 1, respectively. Similar treatments in the presence of biofilm (SBC) resulted in significant (p < 0.001) increase in dissolved and total recoverable As up to 20 and 47 μg L− 1, respectively, exceeding the regulatory As limit. Whether or not, our laboratory-based results truly represent mechanisms operating in disinfected finished water in pipe networks remains to be investigated in the field.

Environmental factors, such as storage time, frequency of bottle reuse and temperature, have been shown to facilitate antimony (Sb) leaching from water- and food-packaging materials. The globally escalating consumption of water packaged in Sb-containing bottles, such as that of polyethylene terephthalate (PET), could increase human daily Sb doses. This study set out to investigate the relationship between drinking-water source, use characteristics, and urinary Sb concentrations (U-Sb) accompanied with survey responses of a healthy (n=35) Cypriot participant pool. One spot urine sample was collected during administration of questionnaire, while a second spot urine sample was collected from the same individual about 7 days later. Urinary and water Sb concentrations were measured with an inductively coupled plasma mass spectrometer. Survey responses showed that bottled water summed over various volumes and plastic types, such as polycarbonate and PET contributed to an average 61% of daily water consumption. Water sources such as tap, mobile stations (explained in a following section), and well water contributed to 24%, 14%, and 2% of an individual's daily water consumption pattern, respectively. Average daily potable water use of both bottled and tap water by individuals consisted of 65% drinking-water, while the remaining 35% was water used for preparing cold and hot beverages, such as, tea, coffee, and juices. A significant (P=0.02) association between per capita water consumption from PET bottles and urinary creatinine-unadjusted concentrations was observed, but this relationship did not remain after inclusion of covariates in a multivariate regression model. In the creatinine-adjusted regression model, only gender (female) was a significant (P

The globally escalating thyroid nodule incidence rates may be only partially ascribed to better diagnostics, allowing for the assessment of environmental risk factors on thyroid disease. Endocrine disruptors or thyroid-disrupting chemicals (TDC) like bisphenol A, phthalates, and polybrominated diphenyl ethers are widely used as plastic additives in consumer products. This comprehensive review studied the magnitude and uncertainty of TDC exposures and their effects on thyroid hormones for sensitive subpopulation groups like pregnant women, infants, and children. Our findings qualitatively suggest the mixed, significant (α = 0.05) TDC associations with natural thyroid hormones (positive or negative sign). Future studies should undertake systematic meta-analyses to elucidate pooled TDC effect estimates on thyroid health indicators and outcomes.

The current in vitro study was designed to investigate the anti-inflammatory, cytotoxic and antioxidant activities of boesenbergin A (BA), a chalcone derivative of known structure isolated from Boesenbergia rotunda. Human hepatocellular carcinoma (HepG2), colon adenocarcinoma (HT-29), non-small cell lung cancer (A549), prostate adenocarcinoma (PC3), and normal hepatic cells (WRL-68) were used to evaluate the cytotoxicity of BA using the MTT assay. The antioxidant activity of BA was assessed by the ORAC assay and compared to quercetin as a standard reference antioxidant. ORAC results are reported as the equivalent concentration of Trolox that produces the same level of antioxidant activity as the sample tested at 20 µg/mL. The toxic effect of BA on different cell types, reported as IC50, yielded 20.22 ± 3.15, 10.69 ± 2.64, 20.31 ± 1.34, 94.10 ± 1.19, and 9.324 ± 0.24 µg/mL for A549, PC3, HepG2, HT-29, and WRL-68, respectively. BA displayed considerable antioxidant activity, when the results of ORAC assay were reported as Trolox equivalents. BA (20 µg/mL) and quercetin (5 µg/mL) were equivalent to a Trolox concentration of 11.91 ± 0.23 and 160.32 ± 2.75 µM, respectively. Moreover, the anti-inflammatory activity of BA was significant at 12.5 to 50 µM and without any significant cytotoxicity for the murine macrophage cell line RAW 264.7 at 50 µM. The significant biological activities observed in this study indicated that BA may be one of the agents responsible for the reported biological activities of B. rotunda crude extract.

A fast-growing bottled water market is occasionally challenged by reports calling for contaminant leaching from water-contact materials (plastics). Our focus was on leaching of antimony (Sb) and brominated compounds expressed by total soluble bromine (Br) measurements, including those of polybrominated diphenyl ethers (PBDE). Studies are lacking on concomitant leaching of two or more inorganic plastic constituents from the same bottle. A market-representative basket survey of bottled water was initiated in Boston, USA supermarkets. Bottled water classes sampled were: i) non-carbonated (NCR), ii) carbonated (CR), and iii) non-carbonated and enriched (NCRE). Plastic bottle materials sampled were: polyethylene terephthalate (PET), high-density polyethylene (HDPE), polystyrene (PS), and polycarbonate (PC). Storage conditions for the 31 bottled water samples were: 23 °C temperature, no-shaking and 12 h/12 h light/dark for 60 days of equilibration. Average Br and Sb concentrations after 60-days of storage followed the order of NCR

Microbial-, and chemical-based burden of disease associated with lack of access to safe water continues to primarily impact developing countries. Cost-effective health risk-mitigating measures, such as of solar disinfection applied to microbial-contaminated water stored in plastic bottles have been increasingly tested in developing countries adversely impacted by epidemic water-borne diseases. Public health concerns associated with chemical leaching from water packaging materials led us to investigate the magnitude and variability of antimony (Sb) and bromine (Br) leaching from reused plastic containers (polyethylene terephthalate, PET; and polycarbonate, PC) subject to UV and/or temperature-driven disinfection. The overall objective of this study was to determine the main and interactive effects of temperature, UV exposure duration, and frequency of bottle reuse on the extent of leaching of Sb and Br from plastic bottles into water. Regardless of UV exposure duration, frequency of reuse (up to 27 times) was the major factor that linearly increased Sb leaching from PET bottles at all temperatures tested (13-47 °C). Leached Sb concentrations (∼360 ng L(-1)) from the highly reused (27 times) PET bottles (minimal Sb leaching from PC bottles

Non-carcinogenic effects in low-level (< 100 μgL(-1)) arsenic (As)-impacted populations, such as the development and progression of type-2 diabetes mellitus (T2DM), are often neglected given the primary emphasis of public health authorities on As carcinogenicity. We gathered studies reporting urinary biomarkers of As exposure (U-As) and biomarkers associated with T2DM and its complications (U-T2DM), such as renal damage, oxidation stress, low-grade inflammation, and endothelial damage. Studied U-T2DM biomarkers were: 8-hydroxy-2'deoxyguanosine, N-acetyl-β-d-glucosaminidase, β2-microglobulin, and albumin. Data was expressed as: either arithmetic means and standard deviations, or geometric means and geometric standard deviations, or correlation coefficients of U-As and U-T2DM. Urinary As concentrations were consistently associated with the aforementioned biomarkers of T2DM pathologic complications. Despite the limited selectivity of the selected T2DM biomarkers, a per unit change in As exposure level was reflected in the corresponding T2DM biomarker urinary concentrations. Our systematic review provides new evidence on the role of environmental As exposures influencing the T2DM disease process. Additional epidemiologic studies onto the association between As and T2DM should incorporate both urinary As and T2DM biomarkers, as suggested in this study, in order to evaluate subclinical effects of low-level As exposures.

Concentrated animal feeding operations around the globe generate large amounts of nitrous oxide (N(2)O) in the surrounding atmosphere. Liquid animal waste systems have received little attention with respect to N(2)O emissions. We hypothesized that the solution chemistry of animal waste aqueous suspensions would promote conditions that lead to N(2)O supersaturation at the liquid/air interface. The concentration of dissolved N(2)O in poultry litter (PL) aqueous suspensions at 25 degrees C was 0.36 microg N(2)O mL(-1), at least an order of magnitude greater than that measured in water in equilibrium with ambient air, suggesting N(2)O supersaturation. There was a nonlinear increase in the N(2)O Henry constants of PL from 2810 atm/mole fraction at 35 degrees C to 17 300 atm/mole fraction at 41 degrees C. The extremely high N(2)O Henry constants were partially ascribed to N(2)O complexation with aromatic moieties. Complexed N(2)O structures were unstable at temperatures > 35 degrees C, supplying the headspace with additional free N(2)O concentrations.

Animal wastewater lagoons nearby concentrated animal feeding operations (CAFOs) represent the latest tendency in global animal farming, severely impacting the magnitude of greenhouse gas emissions, including nitrous oxide (N(2)O). We hypothesized that lagoon wastewater could be supersaturated with N(2)O as part of incomplete microbial nitrification/denitrification processes, thereby regulating the N(2)O partitioning in the gaseous phase. The objectives of this study were: (i) to investigate the magnitude of dissolved N(2)O concentrations in the lagoon; and (ii) to determine the extent to which supersaturation of N(2)O occurs in wastewater lagoons. Dissolved N(2)O concentrations in the wastewater samples were high, ranging from 0.4 to 40.5 microg N(2)O mL(-1). Calculated dissolved N(2)O concentrations from the experimentally measured partition coefficients were much greater than those typically expected in aquatic systems (approximately 0.6 microg N(2)O mL(-1)). Knowledge of the factors controlling the magnitude of N(2)O supersaturation could potentially bridge mass balance differences between in situ measurements and global N(2)O models.

Lead (Pb)-based paints pose a serious health problem to people living in residential settings constructed prior to 1978. Children are at a greater risk to Pb exposure resulting from hand-to-mouth activity in Pb-contaminated residential soils. For soil Pb, the most environmentally friendly, potentially cheap, and visually unobtrusive in situ technology is phytoremediation. However, the limiting factor in a successful phytoremediation strategy is the availability of Pb for plant uptake. The purpose of this study was to establish a relationship between soil properties and the plant-available/exchangeable Pb fraction in the selected Pb-based paint-contaminated residential sites. We selected 20 such sites from two different locations (San Antonio, Texas and Baltimore, Maryland) with varying soil properties and total soil Pb concentrations ranging between 256 and 4,182 mg kg(-1). Despite higher Pb levels in these soils that exceeds US EPA permissible limit of 400 mg kg(-1), it is known that the plant-available Pb pools are significantly lower because of their sorption to soil components such as organic matter, Fe-Mn oxides, and clays, and their precipitation in the form of carbonates, hydroxides, and phosphates. Principal component analysis and hierarchical clustering showed that the potentially plant-available Pb fraction is controlled by soil pH in the case of acidic Baltimore soils, while soil organic matter plays a major role in alkaline San Antonio soils. Statistical models developed suggest that Pb is likely to be more available for plant uptake in Baltimore soils and a chelant-assisted phytoextraction strategy will be potentially necessary for San Antonio soils in mobilizing Pb from complexed pool to the plant-available pool. A thorough knowledge of site-specific factors is therefore essential in developing a suitable and successful phytoremediation model.

A growing body of literature reports 3-nitro-4-hydroxyphenylarsonic acid (roxarsone) degradation in poultry litter (PL) to the more toxic inorganic arsenic (As). Aluminum-based drinking-water treatment residuals (WTR) present a low-cost amendment technology to reduce As availability in PL, similar to the use of alum to reduce phosphorus availability. Batch experiments investigated the effectiveness of WTR in removing roxarsone and inorganic As species from PL aqueous suspensions. Incubation experiments with WTR-amended PL evaluated the effects of WTR application rates (2.5-15% by weight) and incubation time (up to 32 d) at two incubation temperatures (23 and 35 degrees C) on As availability in PL. Batch PL aqueous experiments showed the high affinity of As(V), As(III), dimethylarsinic acid (DMA), monomethylarsonic acid (MMA), and roxarsone for the WTR. The 10% WTR amendment rate decreased As availability in PL by half of that of the unamended (no WTR) PL-incubated samples. The reduction in dissolved As concentrations during incubation of WTR-amended PL samples was kinetically limited, being complete within 13 d. Parallel reductions in roxarsone, As(V), and DMA concentrations were observed with liquid chromatography-inductively coupled plasma mass spectrometry, whereas As(III) and MMA concentrations were always5% of dissolved As. Incubation temperature did not significantly (p0.05) influence dissolved As concentrations in the WTR-amended PL. Potential formation of a copper-containing roxarsone metabolite was considered in PL aqueous suspensions with the aid of electrospray mass spectrometry. Further experiments in the field are necessary to ensure that sorbed As is stable in WTR-amended PL.

Chelation of metals is an important factor in enhancing solubility and hence, availability to plants to promote phytoremediation. We compared the effects of two chelants, namely, ethylenediaminetetraacetic acid (EDTA) and ethylenediaminedisuccinic acid (EDDS) in enhancing mobilized lead (Pb) in Pb-based paint contaminated residential soils collected from San Antonio, Texas and Baltimore, Maryland. Batch incubation studies were performed to investigate the effectiveness of the two chelants in enhancing mobilized Pb, at various concentrations and treatment durations. Over a period of 1 month, the mobilized Pb pool in the San Antonio study soils increased from 52 mg kg −1 to 287 and 114 mg kg −1 in the presence of 15 mM kg −1 EDTA and EDDS, respectively. Stepwise linear regression analysis demonstrated that pH and organic matter content significantly affected the mobilized Pb fraction. The regression models explained a large percentage, from 83 to 99%, of the total variation in mobilized Pb concentrations.

Transverse joints in pavement structures accommodate contraction, expansion, and interruptions in construction. Mechanical load transfer devices, such as dowel bars, are often used in rigid pavements to transfer force from a loaded slab to an adjacent slab. An experimental study was conducted to investigate the effect of concrete strength, dowel diameter (smooth dowels), and joint width on transverse deflections of loaded dowels. Instrumentation was developed to measure the deflected shape of a portion of a dowel embedded in a slab. Laboratory mockups of pavement joints containing concrete with three nominal compressive strengths, three different dowel diameters, and two different joint widths were tested. Results indicated differences between measured dowel deflections and deflected shapes from those predicted by a widely used equation for a prismatic bar resting on an elastic foundation. Agreement between theory and the tests was also found to be quite sensitive to an elastic parameter used in the prediction equation.