201. Tracking spatio-temporal distribution and transmission of antibiotic resistance in aquatic environments by using ESBL-producing Escherichia coli as an indicator.
- Author
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Li, Qi, Zou, Huiyun, Wang, Di, Zhao, Ling, Meng, Min, Wang, Zhongyi, Wu, Tianle, Wang, Shuang, and Li, Xuewen
- Subjects
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DRUG resistance in bacteria , *PULSED-field gel electrophoresis , *SEWAGE disposal plants , *ESCHERICHIA coli , *SPATIO-temporal variation , *WATERSHEDS , *ENTEROBACTERIACEAE - Abstract
Wastewater treatment plants (WWTPs) play an important role in the production, and transmission of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) as a convergence for human, animal, and environmental wastewater. The aim of this study was to investigate the spatio-temporal variation and influencing factors of ARB in different functional areas of the urban WWTP and the connecting rivers for 1-year monitoring using extended-spectrum β-lactamase-producing Escherichia coli (ESBL-Ec) as an indicator bacteria, and to study the transmission patterns of ARB in the aquatic environment. The results showed that ESBL-Ec isolates were identified from the WWTP (n = 219), including influent (n = 53), anaerobiotic tank (n = 40), aerobiotic tank (n = 36), activated sludge tank (n = 31), sludge thickner tank (n = 30), effluent (n = 16), and mudcake storage area (n = 13). The dehydration process can significantly remove the ESBL-Ec isolates; however, ESBL-Ec was still detected in samples collected from the effluent of the WWTP (37.0%). The detection rate of ESBL-Ec was significantly different across seasons (P < 0.05), and ambient temperature was negatively correlated with the detection rate of ESBL-Ec (P < 0.05). Furthermore, a high prevalence of ESBL-Ec isolates (29/187, 15.5%) was detected in samples collected from the river system. These findings emphasize that the high majority of ESBL-Ec in aquatic environments is alarming because it poses a significant threat to public health. Clonal transmission of ESBL-Ec isolates between the WWTP and rivers based on the spatio-temporal scale was observed by pulsed-field gel electrophoresis analysis, ST38 and ST69 ESBL-Ec clone were selected as prioritized isolates for antibiotic resistance monitoring in the aquatic environment. Further phylogenetic analysis showed human-associated (feces, blood) E. coli was the main source contributing to the presence of antibiotic resistance in aquatic environments. Longitudinal and targeted monitoring of ESBL-Ec in WWTPs and the development of effective wastewater disinfection strategies before effluent discharge from WWTPs are urgently required, to prevent and control the spread of antibiotic resistance in the environment. • Higher prevalence of ESBL-Ec was identified in samples collected in spring, winter. • Temperature and humidity were negatively correlated with prevalence of ESBL-Ec. • Clonal transmission of ESBL-Ec based on the spatio-temporal scale was confirmed. • ST38 and ST69 ESBL-Ec were prioritized for antibiotic resistance monitoring. • Human-associated E. coli was the main origin of ESBL-Ec in the WWTP. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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