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Introduction: Platelet additive solutions support ex vivo storage of platelet concentrates used for transfusion. The composition of platelet additive solutions within one generation (i.e., PAS-E) is similar but not identical. Additionally, the platelet additive solution storage bag may contain different plasticizers. This study compares the effect of two PAS-E solutions (SSP+ vs. T-PAS+, stored in a DEHP-containing and DEHP-free bag, respectively) to investigate if both additive solutions are interchangeable for platelet concentrate storage. Methods: Platelet concentrates stored in plasma supplemented with SSP+ or T-PAS+ were compared by using a pool-and-split design. Platelet metabolism was investigated using a blood gas analyzer. The degree of platelet storage lesion was determined by flow cytometry to measure granule release and phosphatidylserine scrambling. Results: The quality of platelet concentrates stored in either SSP+ or T-PAS+ is acceptable as pH decreased only slightly as a function of time. PH remained above 7.2 on exiration day +1 (day 6), which is far above the minimal criterion of 6.4. Platelet storage lesion was comparable between the two study groups with only limited α-granule release and phosphatidylserine surface expression in both groups after storage for 5 days, p = 0.547 and p = 0.825, respectively. Conclusion: This study supports a safe switch between SSP+ and T-PAS+ storage solutions for platelet concentrates despite slight differences in storage solution composition and DEHP content. [ABSTRACT FROM AUTHOR]

Agricultural landscapes are multifunctional and closely connected to the much wider food system. In our review, we explore three specific aspects of modelling approaches for agricultural landscape assessments: (a) how multifunctionality is commonly analysed to support decision-making for sustainable agricultural land management; (b) how the modelling approaches relate to the wider food systems; and (c) how gaps in the existing modelling approaches might be addressed. For this, we identified key elements of agricultural landscape assessments, including ecosystem services (ESS) provided, driving factors, and linkages between crop and livestock production, and to the wider food system. We reviewed 238 publications with respect to these elements. While biodiversity and the ESS ‘water conditions’ and ‘atmospheric composition/conditions’ are widely covered, they are rarely used in combination. Other ESS, such as ‘pest and disease control’, are largely missing. Our results further indicate strong differences between individual approaches regarding model parameterisation and consideration of abiotic, biotic, and management driving factors. Our analysis also shows that the interconnectedness of crop and livestock production is rarely considered and that return flows from the food system are not considered. Furthermore, impacts from the production of external inputs and off-site effects are not considered. Consequently, existing models might overlook trade-offs and synergies between landscape functions. Failure to consider variations in relevant driving factors and food system linkages likely results in studying incorrect levers for change and failing to show decision-makers the full scope of available action. We thus suggest adopting more encompassing modelling approaches to ensure coverage of the full scope of potential actions, whilst hedging against overly costly data requirements by, e.g. employing well-designed sensitivity analyses. In this way, the most relevant components and thus the most important leverage points for interventions can be identified.

BackgroundMutation of the IL2RG gene results in a form of severe combined immune deficiency (SCID-X1), which has been treated successfully with hematopoietic stem cell gene therapy. SCID-X1 gene therapy results in reconstitution of the previously lacking T cell compartment, allowing analysis of the roles of T cell immunity in humans by comparing before and after gene correction.MethodsHere we interrogate T cell reconstitution using four forms of high throughput analysis. (1) Estimation of the numbers of transduced progenitor cells by monitoring unique positions of integration of the therapeutic gene transfer vector. (2) Estimation of T cell population structure by sequencing of the recombined T cell receptor (TCR) beta locus. (3) Metagenomic analysis of microbial populations in oropharyngeal, nasopharyngeal, and gut samples. (4) Metagenomic analysis of viral populations in gut samples.ResultsComparison of progenitor and mature T cell populations allowed estimation of a minimum number of cell divisions needed to generate the observed populations. Analysis of microbial populations showed the effects of immune reconstitution, including normalization of gut microbiota and clearance of viral infections. Metagenomic analysis revealed enrichment of genes for antibiotic resistance in gene-corrected subjects relative to healthy controls, likely a result of higher healthcare exposure.ConclusionsThis multi-omic approach enables the characterization of multiple effects of SCID-X1 gene therapy, including T cell repertoire reconstitution, estimation of numbers of cell divisions between progenitors and daughter T cells, normalization of the microbiome, clearance of microbial pathogens, and modulations in antibiotic resistance gene levels. Together, these results quantify several aspects of the long-term efficacy of gene therapy for SCID-X1. This study includes data from ClinicalTrials.gov numbers NCT01410019, NCT01175239, and NCT01129544.

Fractionation of soil organic carbon (SOC) is crucial for mechanistic understanding and modeling of soil organic matter decomposition and stabilization processes. It is often aimed at separating the bulk SOC into fractions with varying turnover rates, but a comprehensive comparison of methods to achieve this is lacking. In this study, a total of 20 different SOC fractionation methods were tested by participating laboratories for their suitability to isolate fractions with varying turnover rates, using agricultural soils from three experimental sites with vegetation change from C3 to C4 22–36 years ago. Enrichment of C4-derived carbon was traced and used as a proxy for turnover rates in the fractions. Methods that apply a combination of physical (density, size) and chemical (oxidation, extraction) fractionation were identified as most effective in separating SOC into fractions with distinct turnover rates. Coarse light SOC separated by density fractionation was the most C4-carbon enriched fraction, while oxidation-resistant SOC left after extraction with NaOCl was the least C4-carbon enriched fraction. Surprisingly, even after 36 years of C4 crop cultivation in a temperate climate, no method was able to isolate a fraction with more than 76% turnover, which challenges the link to the most active plant-derived carbon pools in models. Particles with density >2.8 g cm−3 showed similar C4-carbon enrichment as oxidation-resistant SOC, highlighting the importance of sesquioxides for SOC stabilization. The importance of clay and silt-sized particles (

Fractionation of soil organic carbon (SOC) is crucial for mechanistic understanding and modeling of soil organic matter decomposition and stabilization processes. It is often aimed at separating the bulk SOC into fractions with varying turnover rates, but a comprehensive comparison of methods to achieve this is lacking. In this study, a total of 20 different SOC fractionation methods were tested by participating laboratories for their suitability to isolate fractions with varying turnover rates, using agricultural soils from three experimental sites with vegetation change from C3 to C4 22–36 years ago. Enrichment of C4-derived carbon was traced and used as a proxy for turnover rates in the fractions. Methods that apply a combination of physical (density, size) and chemical (oxidation, extraction) fractionation were identified as most effective in separating SOC into fractions with distinct turnover rates. Coarse light SOC separated by density fractionation was the most C4-carbon enriched fraction, while oxidation-resistant SOC left after extraction with NaOCl was the least C4-carbon enriched fraction. Surprisingly, even after 36 years of C4 crop cultivation in a temperate climate, no method was able to isolate a fraction with more than 76% turnover, which challenges the link to the most active plant-derived carbon pools in models. Particles with density >2.8 g cm−3 showed similar C4-carbon enrichment as oxidation-resistant SOC, highlighting the importance of sesquioxides for SOC stabilization. The importance of clay and silt-sized particles (

The Century Experiment at the Russell Ranch Sustainable Agriculture Facility at the University of California, Davis provides long-term agroecological data from row crop systems in California's Central Valley starting in 1993. The Century Experiment was initially designed to study the effects of a gradient of water and nitrogen availability on soil properties and crop performance in ten different cropping systems to measure tradeoffs and synergies between agricultural productivity and sustainability. Currently systems include 11 different cropping systems-consisting of four different crops and a cover crop mixture-and one native grass system. This paper describes the long-term core data from the Century Experiment from 1993-2014, including crop yields and biomass, crop elemental contents, aerial-photo-based Normalized Difference Vegetation Index data, soil properties, weather, chemical constituents in irrigation water, winter weed populations, and operational data including fertilizer and pesticide application amounts and dates, planting dates, planting quantity and crop variety, and harvest dates. This data set represents the only known long-term set of data characterizing food production and sustainability in irrigated and rainfed Mediterranean annual cropping systems. There are no copyright restrictions associated with the use of this dataset.

ABSTRACTSustainability certification has been posited as a key governance mechanism to enhance the climate resilience of smallholder farmers. Whilst many certifications now include climate resilience in their standards, their ability to deliver this for smallholders remains untested. We take the case of the 2015–16 drought-shock to cocoa production in Ghana to examine whether certification can enhance smallholder climate resilience. We used a novel transdisciplinary methodology combining participatory outcome definition with household surveys, biophysical measurements, satellite data and counterfactual analysis. Utilising our climate resilience framework, we find that certification has a strong effect on the adoption of basic management, e.g. fertilization, but a weak influence on more complex resilience strategies, e.g. agroforest diversification. Beyond certification, we identify strong regional patterns in resilience. These findings suggest that certification has some potential to enhance climate resilience but greater focus on facilitating diversification and adapting to sub-national contexts is required for improved effectiveness.

ABSTRACT Marine Synechococcus comprise a numerically and ecologically prominent phytoplankton group, playing a major role in both carbon cycling and trophic networks in all oceanic regions except in the polar oceans. Despite their high abundance in coastal areas, our knowledge of Synechococcus communities in these environments is based on only a few local studies. Here, we use the global metagenome data set of the Ocean Sampling Day (June 21st, 2014) to get a snapshot of the taxonomic composition of coastal Synechococcus communities worldwide, by recruitment on a reference database of 141 picocyanobacterial genomes, representative of the whole Prochlorococcus, Synechococcus, and Cyanobium diversity. This allowed us to unravel drastic community shifts over small to medium scale gradients of environmental factors, in particular along European coasts. The combined analysis of the phylogeography of natural populations and the thermophysiological characterization of eight strains, representative of the four major Synechococcus lineages (clades I to IV), also brought novel insights about the differential niche partitioning of clades I and IV, which most often co-dominate the Synechococcus community in cold and temperate coastal areas. Altogether, this study reveals several important characteristics and specificities of the coastal communities of Synechococcus worldwide. IMPORTANCE Synechococcus is the second most abundant phytoplanktonic organism on Earth, and its wide genetic diversity allowed it to colonize all the oceans except for polar waters, with different clades colonizing distinct oceanic niches. In recent years, the use of global metagenomics data sets has greatly improved our knowledge of “who is where” by describing the distribution of Synechococcus clades or ecotypes in the open ocean. However, little is known about the global distribution of Synechococcus ecotypes in coastal areas, where Synechococcus is often the dominant phytoplanktonic organism. Here, we leverage the global Ocean Sampling Day metagenomics data set to describe Synechococcus community composition in coastal areas worldwide, revealing striking community shifts, in particular along the coasts of Europe. As temperature appears as an important driver of the community composition, we also characterize the thermal preferenda of 8 Synechococcus strains, bringing new insights into the adaptation to temperature of the dominant Synechococcus clades.