201. Effective transfer of a 47 kb NDM-1-positive plasmid among Acinetobacter species.
- Author
-
Tzu-Wen Huang, Tsai-Ling Lauderdale, Tsai-Lien Liao, Ming-Chia Hsu, Feng-Yee Chang, Shan-Chwen Chang, Wei Xin Khong, Oon Tek Ng, Ying-Tsong Chen, Shu-Chen Kuo, Te-Li Chen, Jung-Jung Mun, Shih-Feng Tsai, Huang, Tzu-Wen, Lauderdale, Tsai-Ling, Liao, Tsai-Lien, Hsu, Ming-Chia, Chang, Feng-Yee, Chang, Shan-Chwen, and Khong, Wei Xin
- Subjects
ACINETOBACTER infections ,ANTIBIOTICS ,CROSS infection ,DNA ,DRUG resistance in microorganisms ,GENES ,GENETICS ,GENOMES ,HYDROLASES ,MICROBIAL sensitivity tests ,GRAM-negative aerobic bacteria ,PHARMACODYNAMICS - Abstract
Objectives: To investigate the link between two NDM-1-positive Acinetobacter isolates from the same hospital, the plasmid profiles of the isolates were examined. These two isolates were found from a surveillance programme within 3 months from two patients without obvious physical contact or hospitalization time overlap.Methods: Antimicrobial susceptibility tests, genome sequencing of both isolates and plasmid transfer experiments were performed. A comparative study of similar plasmids was performed using BLAST analysis.Results: The antimicrobial susceptibility of the isolates (Acinetobacter soli M131 and Acinetobacter pittii MS32) and their Escherichia coli transconjugants revealed a conjugative plasmid that carried the carbapenem resistance determinant. Eleven plasmids were observed in M131 and three in MS32. Each isolate shared an identical plasmid that carried the blaNDM-1 gene. This 47 271 bp plasmid harbours a conserved blaNDM-1-containing region that is flanked by ISAba125 and ISAba11 elements, and also contains a Ti-type conjugative operon. The plasmid is nearly identical in sequence to those of Acinetobacter isolates from China. In contrast to the mobilization of the blaNDM-1 sequence in Enterobacteriaceae, which is mainly by transposition, this plasmid moves as a whole among Acinetobacter species. Consistently, this plasmid was found to transfer effectively by in vitro conjugation to several Acinetobacter species.Conclusions: The clinical and laboratory findings suggest that Acinetobacter species may serve as a reservoir of this blaNDM-1 plasmid. Our study demonstrates the potential of applying genome sequencing to the surveillance of antimicrobial-resistant bacteria. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF