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Qi Xu, Fen Pan, Yan Sun, Chun Wang, Yingying Shi, Tiandong Zhang, Fangyuan Yu, Hong Zhang Department of Clinical Laboratory, Shanghai Children’s Hospital, Shanghai Jiaotong University, Shanghai, People’s Republic of ChinaCorrespondence: Hong Zhang Department of Clinical LaboratoryShanghai Children’s Hospital, Shanghai Jiaotong University, No. 24, Lane 1400, West Beijing Road, Shanghai 200040, People’s Republic of ChinaTel/ Fax +86 21 6247 4880 Ext 82301Email schjyk2015@126.comPurpose: To determine the epidemiology characteristics of intestinal colonization of carbapenem-resistant Enterobacteriaceae (CRE) among inpatients in a pediatric hospital in China.Methods: A retrospective study was conducted from April to December 2019. Medical records were reviewed to extract the clinical information. Antimicrobial susceptibility was performed by broth microdilution method. Drug resistance determinants and plasmid types were analyzed using polymerase chain reaction (PCR) assays. Multilocus sequence typing (MLST) and Enterobacterial repetitive intergenic consensus sequences PCR (ERIC-PCR) were employed to determine the genetic relationships between strains.Results: A total of 90 CRE strains were isolated, with a fecal carriage rate of 8.6% (90/1052), and mainly distributed in E. aerogenes (n=30), K. pneumoniae (n=25) and E. coli (n=23). More than 50% of CRE colonizers had a history of invasive procedures and antibiotic exposures. As high as 91.1% (82/90) of CRE isolates carried carbapenemase genes, with blaNDM-5 (n=56) being the most common, and mainly found in E. aerogenes (51.8%, 29/56) and E. coli (32.1%, 18/56) isolates, which primarily belonged to ST4 (100%, 29/29) and ST692 (55.6%, 10/18), respectively. Followed by blaKPC-2 (n=12), and all found in K. pneumoniae ST11 isolates. Other carbapenemase genes including blaNDM-1, blaIMP-4 and blaIMP-26. Meanwhile, ESBL genes (blaCTX-M, blaTEM-1 and blaSHV) and AmpC genes (blaDHA-1 and blaEBC) were also detected. All CRE isolates showed high resistance to cephalosporins and carbapenemases (97.8%-100.0%) but remained susceptible to tigecycline (98.9%). IncX3 was a major plasmid type in NDM-containing strains (91.3%), and 91.7% of KPC-2-producing K. pneumoniae harboring IncFII and IncFIB plasmids. The ERIC-PCR revealed that several strains with identical STs were genetically similar.Conclusion: This study revealed a major intestinal colonization of ST4 NDM-5 E. aerogenes, ST11 KPC-2 K. pneumoniae and ST692 NDM-5 E. coli strains among inpatients in a pediatric hospital. Infection control measures should be implemented immediately to prevent the spread of these strains in clinical settings.Keywords: intestinal colonization, hospitalized children, ST4, E. aerogenes, NICU, ERIC-PCR

Xiaofang Jiang,1,* Lin Zhou,2,3,* Lihua Zuo,2,3 Xiaohui Wang,4 Yingying Shi,2,3 Xiangyu Du,5 Jun Zhang,2,3 Liwei Liu,2,3 Zhuolun Li,2,3 Lianping Xue,2,3 Xin Liu,1 Zhi Sun2,3 1Department of Traditional Chinese Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province 450052, People’s Republic of China; 2Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province 450052, People’s Republic of China; 3Henan Key Laboratory of Precision Clinical Pharmacy, Zhengzhou, Henan Province 450052, People’s Republic of China; 4Department of Ultrasonography, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province 450052, People’s Republic of China; 5School of Basic Medical Science, Henan University, Kaifeng, Henan Province 475001, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhi Sun; Lin ZhouDepartment of Pharmacy, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Erqi District, Zhengzhou 450052, Henan Province, People’s Republic of ChinaTel/Fax +86 371 66862570Email sunzhi2013@163.com; fcczhoul@zzu.edu.cnPurpose: Shenkang injection, a traditional Chinese herbal prescription, had been widely used in renal disease due to its perfect curative effect. In this research, a novel, sensitive, accurate and rapid liquid chromatography-tandem mass spectrometric method was developed to simultaneously detect the seven active ingredients in rat plasma of Shenkang injection and investigate its pharmacokinetic behaviors with metabolism profiling meanwhile.Methods: For accurate pharmacokinetic quantitation, a WATERS ACQUITY UPLC® BEH C18 column was used to perform a separation and acetonitrile-water (0.1% formic acid) was selected as mobile phase for gradient elution with a flow rate of 0.20 mL/min. A heated electrospray ionization with selective reaction monitoring mode was used to monitor the precursor-product ion transitions for all the analytes and IS.Results: They all showed good linearity over a wide concentration range (r> 0.996 3) and the lower limit of quantification (LLOQ) was 0.1– 1.0 ng/mL for analytes. The validation parameters were all within the acceptable limits. Furthermore, for metabolism profiling study, metabolites of the seven ingredients were identified from the rat plasma based on the accurate mass and fragment ions. The metabolic pathways mainly focus on reduction, dehydration and conjugation.Conclusion: This study provided an overview of disposition of Shenkang injection, which is highly instructive for better understanding the effectiveness and toxicity of this drug.Keywords: Shenkang injection, pharmacokinetics, metabolism profiling, UHPLC-MS/MS, UHPLC-Q-orbitrap HRMS

Additional file 1: Figure S1. The structure of DBMN. Figure S2. Size (A) and zeta potential (B) distributionof MN, HMN and DBMN tested by DLS. Figure S3. TGA (A) and DSC (B) curves of MN, HMN andDBMN. C) The image of the samples after being subjected to a high temperatureof 1000 °C. Figure S4. UV-Vis absorption spectra of the mainreactants and products. Figure S5. MN and DBMN accumulated over time under the magnetic field. Figure S6. Magnetic responsiveness of T cells after incubatingwith DBMN. Culture plate was shaken every 10 minutes for 0.5 hours. Figure S7. A)The scheme of circulation of DBMN-HEK293T-GFP cells in vitro under the magnetic field. B) Fluorescence image ofcatheter near the magnet (This image was composed of three pictures from top tobottom). Figure S8. The photos of thedevice for verifying the magnetic responsiveness of magnetic cells by IVIS. Figure S9.Fluorescence images of DBMN-HEK293T-GFP cells undermagnetic field, captured by IVIS. Blue dashed box, the magnet. Figure S10.Representative flow cytometry pictures of CD8+ T cells proportion inblood (A), spleens (B) and tumors (C). Figure S11.Representative immunofluorescence images of CD8+T cells and IFN-γ in DBMN-T group. Scale bar, 50 µm. FigureS12.Representative H&E staining photographs of hearts, livers, spleens,lungs and kidneys in each group of mice. Yellow arrow, tumor metastasis site. Scale bar,100 µm. Figure S13. The expression of CD44 on 4T1 cells and E.G7-OVA cells. Left is blank.

PURPOSE: The retinal pigment epithelium (RPE) to Bruch’s membrane (BM) distance around geographic atrophy (GA) was measured using an optical attenuation coefficient (OAC) algorithm to determine if this measurement could serve as a clinical biomarker to predict the annual square root enlargement rate (ER) of GA. DESIGN: A retrospective analysis of a prospective, observational case series. METHODS: Eyes with GA secondary to age-related macular degeneration (AMD) were imaged with swept-source OCT (SS-OCT) using a 6×6 mm scan pattern. GA lesions were identified and measured using customized en face OCT images, and GA annual square root ERs were calculated. At baseline, the OACs were calculated from OCT datasets to generate customized en face OAC images for GA visualization. RPE-BM distances were measured using OAC data from different sub-regions around the GA. RESULTS: A total of 38 eyes from 27 subjects were included in this study. Measured RPE-BM distances were the highest in the region closest to GA. The RPE-BM distances immediately around the GA were significantly correlated with GA annual square root ERs (r = 0.595, p

Understanding gluon density distributions and how they are modified in nuclei are among the most important goals in nuclear physics. In recent years, diffractive vector meson production measured in ultra-peripheral collisions (UPCs) at heavy-ion colliders has provided a new tool for probing the gluon density. In this Letter, we report the first measurement of $J/\psi$ photoproduction off the deuteron in UPCs at the center-of-mass energy $\sqrt{s_{_{\rm NN}}}=200~\rm GeV$ in d$+$Au collisions. The differential cross section as a function of momentum transfer $-t$ is measured. In addition, data with a neutron tagged in the deuteron-going Zero-Degree Calorimeter is investigated for the first time, which is found to be consistent with the expectation of incoherent diffractive scattering at low momentum transfer. Theoretical predictions based on the Color Glass Condensate saturation model and the gluon shadowing model are compared with the data quantitatively. A better agreement with the saturation model has been observed. With the current measurement, the results are found to be directly sensitive to the gluon density distribution of the deuteron and the deuteron breakup, which provides insights into the nuclear gluonic structure., Comment: Final published version Phys. Rev. Lett. 128, 122303

PURPOSE: Widefield (WF) swept source (SS) OCT angiography (SS-OCTA) was used to image diabetic tractional retinal detachments (TRDs) before and after pars plana vitrectomy (PPV). The clinical utility of SS-OCTA was assessed. METHODS: Patients with diabetic TRDs were imaged prospectively with SS-OCTA. Ultrawide field (UWF) imaging was obtained when possible. Post-operative WF SS-OCTA imaging was performed. RESULTS: From January 2018 through December 2019, 31 eyes of 21 patients with diabetic TRDs were imaged. WF SS-OCTA en face images captured all areas of TRD and fibrovascular proliferation within the posterior pole that were visualized on UWF imaging. OCTA B-scans revealed the vascularity of preretinal membranes and identified areas of vitreoretinal traction and posterior vitreous detachment. Ten eyes underwent PPV. Post-operative SS-OCTA imaging demonstrated removal of fibrovascular membranes, relief of traction, and resolution of TRDs. Retinal ischemia before and after surgical repair appeared similar. CONCLUSIONS: All clinically relevant features of diabetic TRDs were identified at baseline and assessed longitudinally after PPV using WF SS-OCTA, which showed resolution of vitreo-retinal traction and no apparent change in the status of retinal perfusion after surgery. If the media are clear and fixation is adequate, WF SS-OCTA is likely the only imaging modality needed for the diagnosis and longitudinal evaluation of diabetic TRDs. SUMMARY STATEMENT: Wide field swept-source OCT angiography revealed all clinically relevant features of diabetic tractional retinal detachments. There were no apparent changes in macular perfusion after surgical repair.