201. SSR Marker-based Diversity among Elite Maize (Zea mays L.) Inbred Lines and Parents of Elite Single Cross Hybrids with Known Heterotic Groups.
- Author
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SIDDU, C. B., RAMESH, S., BASANAGOUDA, G., and KALPANA, M. P.
- Subjects
SINGLE parents ,MICROSATELLITE repeats ,GENETIC variation ,GENE frequency ,INBREEDING - Abstract
Exploiting heterosis through F
1 hybrids is considered one of the most effective methods to increase maize productivity. However, developing successful heterotic hybrids requires the use of the combination of diverse inbred parents to develop breeding populations (BPs) and desirable testers to identify new inbred lines for use in developing heterotic hybrids. As step towards this, the objectives of the present investigation were to (i) assess genetic diversity among elite inbred lines using simple sequence repeat (SSR)-based markers and (ii) to identify most desirable combinations of inbred lines. Sixteen lines which included eleven elite inbred lines with high general combining ability and five parents of three commercialised elite single cross hybrids - Hema, Nithyashree and MAH 14-5 were used in the study. These lines were genotyped using 132 SSR markers distributed across the maize genome. The results revealed polymorphism of hundred markers. The number of alleles per locus ranged from 2 to 8. Narrow difference between average (Na ) (3.31) and effective (Ne ) (2.40) number of alleles per locus suggest near-equal frequency of alleles detected at each poly morphic locus. This is amply reflected from high correlation of Na and Ne with polymorphic information content (PIC). Thirty-three markers with ≥3 alleles (multi-allelic) were more informative than bi-allelic markers as reflected from high magnitude of PIC. A wide range of the estimates of dissimilarity indices between pairs of inbred lines suggested differential frequency of alleles at the polymorphic SSR markers between the inbred lines. The best triplet combinations of two inbred parents (with high dissimilarity indices) and a tester (with high dissimilarity indices between parents and the tester) were identified to maximize the chances of recovering new elite inbred lines for use in heterotic hybrids. [ABSTRACT FROM AUTHOR]- Published
- 2023