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Phytase is enzyme which hydrolysis phytic acid to anorganic phosphate and myo-inositol pentakis-, tetrakis-, tris-, bis-, and monophosphate. The use of phytase in feed industry can overcome environment and nutrition problems which were arisen from unmetabolism phytic acid or its salt by poultry, swine and fish. The feed industry needs a thermostable enzyme due to the need of high temperature in pelleting process, i.e. 81 °C. By using thermostabile phytase, the pelleting process will not affect the enzyme activity. Thermostabile phytase can be isolated from microorganism live in hot spring water or volcano crater. In this study, the screening of thermophylic microorganism having thermostabile phytase activity in Ijen Crater, Banyuwangi, has been done. From this process, it was obtained 33 isolates that produce phytase enzyme. Isolate was code by AP-17 yields highest phytase activity, that is 0.0296 U/mL, so this isolate was choosen for further study. The activity of crude phytase enzyme was measured based on the amount of anorganic phosphate that was produced in enzymatic reaction using UV-VIS spectrophotometer at 392 nm. Based on morphology test to identify the gram type of microorganism, isolate AP-17 has a bacill cell type and identified as positive gram bacteria. This isolate was assumed as Bacillus type. Keywords: Phytase, thermophilic microorganism, phytase activity

This research’s aim is to increase the problem solving skill of the students in X-A of SMA Negeri Sragen Billingual Boarding School Gemolong academic Year 2013/2014. The matter is about Enviroment Pollution through Problem Based Learning application by using local area potention. This research is a class action research which consists of three cycles. Each cycle contains of planning, implementing, observing and reflecting. The research subject is the students in X-A of SMA Negeri Sragen Billingual Boarding School Gemolong academic Year 2013/2014. Data of research were obtained by test and non test technique. Data validation were used triangulation of methods and triangulation of observers. The obtained data were analyzed using descriptive technique.The results of this research showed that according triangulation the average percentage for IDEAL problem solver. IDEAL problem solver design measured with performance assement consist of IDEAL process and IDEAL product. The average of IDEAL problem solver become value of problem solving skill for every student. The result of the research shows that the implementation of problem based learning increase the students’ problem solving skill that covers the IDEAL problem solver. The total average of IDEAL problem solver in the X-A class SBBS increased from 42,0 move up into 85,7 score. This research’s conclusion is the implementation of problem based learning can increase the problem solving skill of the students in of SMA Negeri Sragen Billingual Boarding School Gemolong academic Year 2013/2014 about enviroment pollution material. Key Words: Problem based learning, problem solving skill

The purposes of this research are to increase quality of biology learning considered by utility of facilities in the classroom, classroom climate, and student motivation of achievement in class XI IPA 1 at State Senior High School 2 Surakarta in academic year 2013/2014. This research was a classroom action research which performed in two cycles. Each cycle consisted of 4 phases, that is planning, action, observation, and reflection. Subject of research is students of XI IPA 1 at State Senior High School 2 Surakarta. Data of research was obtained by test and non test technique. Data validation was used triangulation of methods and triangulation of observers. The obtained data were analyzed using descriptive technique. The results of this research showed the average percentage for classroom climate pre cycle (62,59 %), cycle I (75,86 %), and cycle II (82,92 %); student motivation of achievement pre cycle (63,22 %), cycle I (74,88 %) and cycle II (78,62 %); and utility of learning facilities in the classroom have been optimally, that are usage of microscope and other learning media included LCD, pictures, and video as a media to support learning activities. The results showed that the average percentage quality of biology learning aspect such as pre cycle (62,90 %), cycle I (75,37 %), and cycle II (80,77 %). The level increase of quality aspect of biology learning from pre cycle to cycle II is 17,87 %. The conclusion of this research is that the implementation of experiential learning model can improve quality of biology learning considered by utility of facilities in the classroom, classroom climate, and student motivation of achievement in class XI IPA 1 State Senior High School 2 Surakarta in academic year 2013/2014. Key Words: Experiential learning, Quality of biology learning

The aim of this research is improve quality of biology learning for teacher performance, conditioning class, student attitude in class, and student motivation of learning in student grade of X-E SMA Negeri 1 Kartasura academic year 2011/2012. This research refers to The Classroom Action Research with subject were 39 students in grade of X-E SMA Negeri I Kartasura. The sources of data consist of information from teacher and student, places and events that happened during teaching and learning process, and also some documents. The data of research is collected through observation sheet, questionnaire and interview. The research uses triangulation as validation data. The research uses descriptive as analytic data. The result of this research shows that there has been improvement percentage of the quality of biology learning in every aspect which includes teacher performance, conditioning of class, student attitude, and student motivation of learning. The percentage’s mean of the questioner result in sequence I is conditioning of class 72,56%; student attitude 65,14%; student motivation of learning 75,02%, and of observasi data are teacher performance 83,33%; conditioning of class 71,54%; student attitude 63,37%; student motivation 75%. The reflection of the first sequence shows that students having low of argument explanation in the learning goal, so the researcher gave further treatment in sequence II. In the second sequence the percentage’s mean of the questioner result is teacher performance 93,33%; conditioning of class 82,69%; student attitude 86,08%; student motivation 86,22%, in this cycle every quality of biology learning aspects improved in line with the target. It can be concluded from the research that Contextual Teaching Learning (CTL) approach application accompained by Subject Specific Pedagogy (SSP) can improve quality of biology learning of students in grade X-E SMA Negeri 1 Kartasura academic year 2011/2012. Key Words: Quality of biology learning, Contextual Teaching Learning, Subject Specific Pedagogy

Tribadi, Suranto, Sajidan. 2009. Variation of morphological and protein pattern of cassava (Manihot esculenta) varieties of Adira1 and Cabak makao in Ngawi, East Java. Nusantara Bioscience 2: 14-22. This research is intended to find out the morphological and anatomical variation as well as the protein band pattern of cassava (Manihot esculenta Crantz) widely spread in three different areas of height. The sample collecting is done using simple random sampling in the three different areas of height that is 50, 300, 1000 meters asl in Ngawi District, East Java while the analysis of protein band pattern is done using SDS-PAGE. The result of the research of morphology and anatomy is analyzed descriptively and presented in the form of tables, histograms, and figures. The analysis of protein band pattern is done using quantitative and qualitative analysis that is based on the appearance or not the gel band pattern by counting the molecular weights based on code marker S 8445 and qualitative method based on the quality of the band formed. The band pattern formed is istimated and presented in the form of zimogram. The result of the research shows that the height of the cultivating site very much influences toward variations of root, stem and leaf morphology. The longest root is at 50-meter heights asl (Cabak makao local variety, the widest stem diameter is at 50 meters asl (Cabak makao local variety) the longest leaf and branch is at 300 meters asl (Cabak makao local variety) and 1000 meters asl (Cabak makao local variety). There is no difference of anatomy in the root, stem, and leaf and no difference of protein band pattern either in Adira1 or Cabak makao local variety.

In the 21st century, the pre-service science teachers faced on extremely global competitiveness, globalization, technologically driven by information and rapidly media-saturated that needs appropriate skills to meet these challenges. The aims of this study is to identify pre-service science teacher perception about high order thinking skills (HOTs) in 21st century. This study employed quantitative design using a survey research method involved 120 pre-service science teachers from Tanjungpura University. The results of this study indicated that prospective science teachers have an awareness of the importance of HOTs and their also knowing that the learning do emphasized on the aspects of HOTs is essential to face the challenges of the 21st century. It is indicated by the mean score of pre-service science teacher perception about the important of HOTs to meet the challenges in the 21st century (M = 4.29, SD = 0.61) and the skills of HOT that their will be required to becomes a teacher in the 21st century (M = 4.31; SD = 0.47) in the high level. This result provides the reasons why we need cultivating a positive awareness of the importance of HOTs in order to fostering the need for teaching that emphasizes the aspects of HOTs during pre-service education.