201. Molecular cloning of a human cDNA for the 41-kDa phosphoribosylpyrophosphate synthetase-associated protein
- Author
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Toshiharu Ishizuka, Hiroyuki Iwahana, Rumi Katashima, Takashi Yamaoka, Mitsuo Itakura, Miwa Fujimura, and Masamiti Tatibana
- Subjects
DNA, Complementary ,Molecular Sequence Data ,Biophysics ,Biology ,Molecular cloning ,Adenocarcinoma ,Biochemistry ,Cell Line ,Structural Biology ,Complementary DNA ,Genetics ,Ribose-Phosphate Pyrophosphokinase ,Tumor Cells, Cultured ,Animals ,Humans ,Northern blot ,Amino Acid Sequence ,Carcinoma, Small Cell ,Cloning, Molecular ,Peptide sequence ,Gene ,Southern blot ,Expressed sequence tag ,Base Sequence ,Sequence Homology, Amino Acid ,Intracellular Signaling Peptides and Proteins ,Proteins ,Fibroblasts ,Molecular biology ,Rats ,Open reading frame ,Blotting, Southern ,HeLa Cells - Abstract
A human cDNA encoding 41-kDa phosphoribosylpyrophosphate (PRPP) synthetase (PRS)-associated protein (PAP41) was cloned from two expressed sequence tag (EST) clones having the nucleotide similarity of 61.5 and 70.0% to human PAP39 cDNA. The predicted open reading frame of 1107 base pairs (bp) has the nucleotide identity of 91.8% to rat PAP41 and encodes a protein of 369 amino acids with a calculated molecular weight (MW) of 40,925. The deduced amino acid sequence exhibits the 98.9% identity to rat PAP41 and 72.2, 50.6, and 50.0% identity with human PAP39, PRS I, and PRS II, respectively, but lacks the PRPP binding site. Southern blot analysis suggested that the PAP41 gene exists as a single copy in the human genome. The single PAP41 mRNA of about 2.1 kb was shown to be present in five human cell lines by Northern blot analysis.
- Published
- 1998