Search

Your search keyword '"Reinhild Klein"' showing total 204 results
Start Over Author "Reinhild Klein"
204 results on '"Reinhild Klein"'

201. High antibody levels against mouse laminin with specificity for galactosyl-(alpha 1-3)galactose in patients with inner ear diseases

Author

Dietrich Plester, Reinhild Klein, Rupert Timpl, Fortunato R. Zanetti, and Peter A. Berg

Subjects
Adult, Male, Immunoblotting, Labyrinth Diseases, Radioimmunoassay, Fluorescent Antibody Technique, Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Disaccharides, Epitope, 03 medical and health sciences, Epitopes, Mice, 0302 clinical medicine, Laminin, Antibody Specificity, medicine, Animals, Humans, 030223 otorhinolaryngology, Laminin binding, medicine.diagnostic_test, biology, business.industry, General Medicine, medicine.disease, Titer, Otorhinolaryngology, 030220 oncology & carcinogenesis, Immunology, biology.protein, Sensorineural hearing loss, Female, Antibody, business
Abstract

Sera of 413 patients with inner ear diseases were examined for ELISA binding to mouse laminin and compared to a normal group. Strongly enhanced antilaminin antibody reactions were observed in a large number of patients with sensorineural hearing loss (68%), tinnitus (60%), and sudden deafness (46%), but not in those with Meniere's disease (14%) or normal individuals (8%). Absorption experiments demonstrated that these antibodies are also responsible for the antisarcolemmal antibody pattern in the immunofluorescence test and for the reaction with pig kidney microsomes in the ELISA. Immunochemical studies showed that laminin binding of high or low titer in sera from patients and normal individuals is due to the Galα1-3Gal epitope present in N-linked oligosaccharides of mouse laminin. No reactions were observed with human laminin, which lacks this epitope. The findings suggest that high levels of antibodies against carbohydrate structures are triggered by persistent infections, providing some new insights about the possible cause of these diseases.

Published
1989

202. Antimitochondrial antibodies in primary biliary cirrhosis

Author

Reinhild Klein, P.A. Berg, and Jutta Lindenborn-Fotinos

Subjects
medicine.medical_specialty, Pathology, Immunodiffusion, Biliary cirrhosis, Fluorescent Antibody Technique, Gastroenterology, Autoimmune Diseases, Epitopes, Primary biliary cirrhosis, Immunopathology, Internal medicine, medicine, Humans, Antigens, Connective Tissue Diseases, Autoantibodies, Hepatitis, Chronic, Hepatitis, Hepatology, biology, business.industry, Liver Cirrhosis, Biliary, Hepatobiliary disease, Complement Fixation Tests, medicine.disease, Prognosis, Mitochondria, Organ Specificity, biology.protein, Antibody, business
Abstract

Caracterisation de l'antigene M2, marqueur de la cirrhose biliaire primitive. Autres anticorps decelables dans d'autres affections hepato-biliaires

Published
1986

203. Immunologic findings in workers formerly exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin and its congeners

Author

Christina Koitka, Dietrich Fenner, Alfred Manz, Reinhild Klein, Axel Muttray, Peter A. Berg, Olaf Päpke, George W. Lucier, Dieter Flesch-Janys, Anne Thews, Donald G. Patterson, Michael Pietsch, Christopher J. Portier, Winfried Prellwitz, Johannes Konietzko, Lutz Edler, Larry L. Needham, Christoph Huber, W. Ehrenthal, Dirk M. Rose, and Detlev Jung

Subjects
Adult, Male, Polychlorinated Dibenzodioxins, Health, Toxicology and Mutagenesis, Polychlorinated dibenzodioxins, Lymphocyte proliferation, Lymphocyte Activation, Cohort Studies, chemistry.chemical_compound, Immune system, Immunity, Occupational Exposure, medicine, Chromates, Tetanus Toxoid, Humans, Pesticides, Phytohemagglutinins, Furans, Aged, Immunity, Cellular, biology, Tetanus, Public Health, Environmental and Occupational Health, Autoantibody, Middle Aged, medicine.disease, Vaccination, chemistry, Chemical Industry, Immunology, Antibody Formation, biology.protein, Female, Antibody, Research Article
Abstract

One hundred ninety-two workers in a German pesticide factory who were exposed to polychlorinated dibenzodioxins and -furans (PCDD/PCDF) were investigated for former and present diseases and laboratory changes of the immune system. Moreover, in a subgroup of 29 highly exposed and 28 control persons, proliferation studies were performed. In addition to assays such as blood count, immunoglobulins, serum electrophoresis, monoclonal bands, surface markers, autoantibodies, and lymphocyte proliferation, two new methods, the rise of tetanus antibody concentration after vaccination and the in vitro resistance of lymphocytes to chromate, were used to diagnose the morphologic and functional state of the immune system. There was no stringent correlation of actual PCDD/PCDF concentrations with the occurrence of infections or with one of the immune parameters. In addition, outcomes of the tetanus vaccination and the chromate resistance test were not correlated with PCDD/PCDF. However, the chromate resistance of lymphocytes stimulated by phytohemagglutinin of highly exposed persons was significantly lower than that for the control group. These findings indicate that the function of lymphocytes can be stressed and possibly impaired by high exposure to PCDD/PCDF. Images Figure 2

204. High incidence of antibodies to lens proteins in sera from patients with uveitis.

Author

Doycheva D, Preuss B, Klein R, and Zierhut M

Subjects
Blotting, Western, Enzyme-Linked Immunosorbent Assay, Humans, Incidence, Scleritis blood, alpha-Crystallins immunology, beta-Crystallins immunology, Autoantibodies blood, Autoantigens immunology, Crystallins immunology, Uveitis blood
Abstract

Background: Uveitis is an intraocular inflammatory disease in which autoimmune reactions have been discussed in playing an important role. Many data in this respect derived from the animal model of "experimental autoimmune uveitis", where several organ-specific autoantigens have been described such as the retinal S-antigen and the interphotoreceptor retinoid-binding protein. However, their diagnostic and pathogenic role in humans has been controversially discussed. In recent studies, the possible relevance of betaB1-crystallin, present in lens and ciliary body, has been outlined. We, therefore, wanted to analyse whether sera from patients with uveitis might contain antibodies to lens proteins, Methods: Human lenses from cadaveric eyes were shock frozen, homogenized, and resuspended. The resulting suspension (human lens protein fraction--HLPF) was analyzed for antigenicity by ELISA and Western blotting with patients' sera. A total of 165 patients with uveitis, 54 patients with scleritis and episcleritis, 56 patients with other eye diseases, and 112 healthy blood donors were studied., Results: Twenty-six (49%) of the 53 patients with anterior uveitis, 17 (32%) of the 53 patients with intermediate uveitis and 7 (22%) of 32 patients with posterior uveitis reacted in the ELISA with the HLPF. Antibodies to lens antigens were detected in one-third of patients with panuveitis and retinal vasculitis. In contrast, only 12% of the healthy blood donors were positive in the ELISA. The number of patients with an autoimmune response to alpha-crystallins in Western blot predominated in all investigated groups., Conclusions: These data indicate that antibodies to lens proteins occur in a high incidence in sera from patients with uveitis. Forty-nine percent of the patients with anterior uveitis and only 12% of healthy controls were positive in the ELISA. In our groups of patients and controls the autoantibodies reacted in the Western blot predominantly with alpha-crystallin. Further studies are required to analyze in more detail the clinical and etiopathogenetic relevance of the antilens antibodies in uveitis.

Published
2007
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results