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A retrospective study involving 14 pet rabbits histopathologically diagnosed with malignant peripheral nerve sheath tumors (MPNSTs) was conducted. The age at diagnosis was 4-12 years, with a median age of 8.6 years. All rabbits had solid subcutaneous tumor masses in varied locations. Surgical excision of the tumors was performed in all cases. Recurrence was observed in 10 cases (71%), and postoperative metastasis to the lung was suspected in 4 cases (29%). The postoperative mean and median survival times were 11 months and 9 months, respectively. Hence, MPNSTs should be considered in the differential diagnosis for subcutaneous masses in rabbits and it is essential to inform the owners of the potentially high recurrence and metastasis rates. [ABSTRACT FROM AUTHOR]

Copyright of Harran University Journal of the Faculty of Veterinary Medicine / Harran Üniversitesi Veteriner Fakültesi Dergisi is the property of Harran University, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

An experiment was carried out to investigate the effects of dietary substitution of soybean meal in different ratios by dried Azolla (Azolla pinnata) on productive and reproductive performances, hematological and serum traits, and economic efficiency of rabbit does as well as semen quality of males. Forty mature does and eight males of Black Balady rabbits aged seven and eight months were assigned to 1 of 4 dietary groups: 0 (control), 20, 30, and 40% of soybean protein substituted with dried Azolla protein. Data were analyzed using repeated measures of statistical software computer program package. There were no significant differences among groups in number of services per conception as well as parturition intervals (days) with a superiority of 30% group over the control and other two groups. Average litter weight was significantly (P<0.05) superior in the 30% replacement group. There were no significant differences in live body weight either at birth or at weaning among the four tested groups and the 30% replacement groups recorded the highest significant daily weight gain of bunnies during the whole experimental period. All Azolla groups recorded better results in the studied traits of bucks' semen compared to the control. Azolla diets did not show any adverse effects on the studied blood parameters. The 30% replacement of the soybean protein group showed the best economic return compared to the other two replacement groups and the control. In conclusion, Azolla can safely and economically replace soybean protein at the rate of 30% in adult female rabbits' diets. [ABSTRACT FROM AUTHOR]

Copyright of Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica is the property of West Pomeranian University of Technology in Szczecin and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Simple Summary: In order to reduce mortality rates during the suckling period, increasing the kit's weight during the first days of life could be a selection criterion in rabbit meat breeding programs. The response to selection is directly related to the heritability of the selected trait. The estimates of heritability (h2) for individual weight were low during the first days of life (0.11 at birth, 0.16 at 5 days, and 0.17 at 7 days) and moderate in the preweaning period (0.21, 0.21, 0.24, and 0.21 at 14, 21, 28, and 35 days, respectively). The weight of the kit at birth showed a strong and positive genetic correlation with weights at 5 and 7 days of age (higher than +0.70). However, the correlation was comparatively low in relation to the remaining weight measurements (less than +0.54). Notably, genetic correlations of weight at 5 and 7 days with the rest of the weight measurements were higher than +0.83. In conclusion, selection for body weight at 5 or 7 days of age would have a significant impact on body weight at birth and at weaning, consequently reducing preweaning losses. The preweaning weight of kits has been related to their mortality during the suckling period. Selecting rabbit kits for individual body weight in the first days of life could be interesting; however, better knowledge of body weight's heritability during the preweaning period is necessary to determine the opportune moment for selection. A total of 1696 growth records of kits from 81 females of the ITLEV2006 synthetic line were analysed in order to estimate the genetic and non-genetic parameters for individual body weight at birth as well as at 5, 7, 14, 21, 28, and 35 days of age. The estimates of heritability (h2) for individual weight were between low (0.11 at birth, 0.16 at 5 days, and 0.17 at 7 days) and moderate (0.21, 0.21, 0.24, and 0.21 at 14, 21, 28, and 35 days, respectively). Weight at birth showed a strong and positive genetic correlation with weight at 5 days (+0.79) and 7 days of age (+0.78), but the correlation was low for the rest of the weight measurements (+0.41, +0.49, +0.54, and +0.54 with weight at 14, 21, 28, and 35 days, respectively). Weight at 5 days and 7 days displayed strong and positive genetic correlations with the rest of the weight measurements (higher than +0.83). The values of the common litter effect (c2) were high, and they increased with age from 0.43 at birth to 0.66 at 35 days of age. The values of the maternal permanent effect (p2) were low compared to those of the common litter effect (c2), varying between 0.04 and 0.11. In conclusion, opting to select for body weight at 5 or 7 days of age would yield a greater response compared to selecting for birth weight. This approach would indirectly increase the kits' weight at birth and at weaning, thereby reducing preweaning losses. [ABSTRACT FROM AUTHOR]

Raising rabbits has vital sustainable development features, as a sustainable development system of meat production strongly relies on its efficacy. The development system of meat production is correlated with the animals' capability for converting feed to meat. Weaning possess a marked stress on rabbits, because of lack of their normal caecal microbiota, hence young rabbits became more vulnerable to diseases. Numerous antibiotics alternatives have been successfully utilized, such as herbal medicines as natural supplements in animal feed. The leaves of Moringa Oleifera could potentially be utilized as a growth promoter due to their antimicrobial activity, antioxidant properties, hepato-protective effect, preservation of renal function, and hypocholesterolemic activity. Twelve recently weaned V-Line rabbit bunnies about four weeks of age and average body weight of 0.74±0.07 were separated equally to feed either normal-prepared rabbit feed (Control group (C); n=6) or moringa-prepared feed (Moringa Oleifera group (MO); n=6) for 4 weeks. Performance (feed intake, body weight, and weight gain), behavior, and serum biochemical analysis (oxidative stress, liver, and kidney function) were assessed. Results revealed that the incorporation of MO in recently weaned rabbit feed enhanced their performance and behavior, as well as lipid peroxidation and glutathione didn't differ significantly and preserved liver and kidney function. In conclusion, MO plays a crucial role in weaning stress alleviation via their antioxidant, hepatic and renal function preservation and their positive effects on rabbit bunnies' performance and behavior. Hence, the data suggests the use of MO leaves as a feed additive for rabbit bunnies to alleviate weaning stress. [ABSTRACT FROM AUTHOR]

The aim of this work was to analyse the effect of incubation of a rabbit insemination dose (ID) with dimethyl sulfoxide (DMSO) on reproductive parameters (kindling rate, number of live-born and stillborn kits, and birth weight of kits). The experiments were carried out using two rabbit breeds. Each ejaculate was diluted to a concentration of 40 to 50 million sperm/0.5 ml ID. DMSO was added to the ID in a concentration of 0.5 M. The control ID did not contain any DMSO. Kindling rates obtained from two different breeds (P1, P2) showed minor improvement (+4.36% P1; +4.06% P2) compared to the controls, but without statistically significant differences. The number of live-born kits per litter obtained from breeds P1 and P2 showed minor improvement (P1 +0.61; P2 +0.13) compared to the control groups (p > 0.05). The weight of live-born kits was higher in the experimental groups (+3.85 g P1 = p = 0.001; +1.18 g P2 = p>0.05) than in the control groups. Monitoring of sperm quality indicators (1, 3 and 6 hours incubation) in in vitro conditions (CASA - Computer Assisted Semen Analysis) showed the best results in the control group that was not treated with DMSO. The data obtained in our experiments suggest positive effects of DMSO incubation on reproduction parameters, especially in vivo. [ABSTRACT FROM AUTHOR]

The purpose of the study was to determine the influence of breed on the growth, slaughter traits, meat quality traits and meat texture parameters of Blanc de Termonde and Flemish Giant rabbits. Young animals were weaned at 35 days of age. Their weight was recorded from birth to 84 days of age at 7-day intervals. The rabbits were slaughtered between 85 and 90 days of age. Hot carcass weight, cold carcass weight and giblets weight (liver, lungs, heart, and kidneys) were recorded. The pH and colour of the longissimus lumborum and biceps femoris muscles were measured at 45 minutes and 24 hours after slaughter. Shear force and texture parameters were measured. Flemish Giant rabbits attained a higher final body weight but a lower dressing out percentage than Blanc de Termonde rabbits. Carcasses of Blanc de Termonde rabbits had higher loin weight and fat content in the carcass. The effect of breed was found to be significant for some components of meat colour: yellowness (b*) at 45 minutes and lightness at 24 hours post mortem. The effect of sex was found to be significant for meat acidity and the b* component at 45 minutes after slaughter and for redness (a*) at 24 hours post mortem. There were statistically significant differences between Flemish Giant and Blanc de Termonde rabbits in shear force and texture profile analysis (TPA). The sex of the animals affected only the chewiness parameter in the TPA of rabbit meat. Blanc de Termonde and Flemish Giant rabbits attained a high final body weight and dressing out percentage and optimal meat quality traits, and therefore can be recommended as breeding material for the production of slaughter rabbits. [ABSTRACT FROM AUTHOR]

Background: Gingiva is a delicate tissue that protects alveolar bone against external stimuli. Gingival breaks expose alveolar bone, and the fast wounds heal the betterment of the teeth frame. We aimed to compare the influence of omega-3 on the gingival healing period after trauma and the potential involvement of cytokeratin-19 protein expression. Methods: A total of 18 rabbits were used, after trauma-induced, these rabbits were subdivided into three groups of 6 each. Group 1 is the control group that received normal saline only, the second group received omega 3 for 10 days before the trauma, while the third group received omega 3 supplements for 10 days before the gingival injury and continued receiving them for an additional 10 days afterwards. For each time point (third and seventh day of healing), 3 rabbits were sacrificed, and tissue was collected for total antioxidant capacity and malondialdehyde measurement. Tissue fixed for histopathology and immunohistochemistry analysis. Results: Omega 3 has shown improvement in the healing process regarding the period of healing, antioxidant parameters, and intensity of cytokeratin-19 expression compared to the control group. Moreover, rabbit groups exposed to omega 3 before trauma has shown better healing response compared to those initiated therapy with trauma. Conclusion: Omega 3 is notably beneficial when used for both prevention and treatment since it reduces oxidative stress and improves healing processes measured by increased expression of cytokeratin-19 in epithelial tissue. Omega 3 can therefore be thought of as a possible choice for the treatment of gingival wounds and gingivitis. [ABSTRACT FROM AUTHOR]

This study highlights the current status of rabbit production, potential challenges facing the industry and the prospects for rabbit farming in Dodoma city, Tanzania. Data from 60 individuals keeping rabbits were collected through a structured questionnaire and direct observation. Results showed that 60% of the respondents were men and 40% women. Rabbits were kept mainly as a source of meat for families and income generation. Main sources of rabbit stock were local breeders (55%) and missionaries (25%), while the remainder were from agricultural institutes. The dominant rabbit breeds reared were California white (50%), New Zealand white (30%), Chinchilla (10%) and Dutch (10%). Ninety-five percent of the farmers kept rabbits in cages, while 5% confined them at night and let them out during the day to forage for feed. Fifteen percent of the respondents fed commercial rabbit pellets, 5% vegetables and kitchen waste only, whereas 80% fed both rabbit pellets, vegetables and kitchen waste. The number of rabbits kept by an individual farmer ranged from 5 to 180, with mean stock size of 22.80±11.20 (mean±standard deviation). Rabbits were sold at 8 to 12 wk of age and each rabbit was sold for 20 000 to 30 000 Tanzanian shillings (8.00 - 12.00 USD). Major challenges in rabbit production were lack of feed resources, lack of readily available market, poor quality of breeding stock, low knowledge of rabbit production techniques and lack of technical support from extension services. Although rabbit farming in Dodoma is carried out at subsistence level, it is associated with a decrease in poverty among urban and peri-urban farmers. In conclusion, rabbit production in urban and peri-urban areas of Tanzania has the potential to contribute to food and nutrition security and poverty alleviation, thus improving living standards. [ABSTRACT FROM AUTHOR]

Copyright of Acta Veterinaria Brasilica is the property of Acta Veterinaria Brasilica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study was designed to evaluate the efficacy of xylazine-ketamine anesthesia on reflexes and vital signs during and after tendon surgery in rabbits. Twenty healthy adult male rabbits (2-2.5 kg) were randomly assigned to two groups Xylazine-ketamine (XK) group and ketamine (K) group. Intramuscular injections of xylazine (5 mg/kg) and ketamine (35 mg/kg), were given to rabbits. The clinical parameters including rectal temperature, heart rate, and respiratory rate were estimated prior to injection 0, 5, 15, 30, 45, 60, 75, 90, and 120 minutes post-anesthetic injection, As well as reflexes ear pinch reflex, and pedal reflex were measured before and after anesthetic injection. In the XK group, we observed that rectal temperature increased considerably (P < 0.05) at 5 and 15 minutes after induction and then gradually fell to preanesthetic control values. During the anesthetic phase, both groups' heart rates and respiration rates were reduced significantly. In XK-injected rabbits, the return of reflexes was delayed compared to K injected groub. Surgical anesthesia in XK group lasted longer than K group. The XK combination provided sufficient anesthesia for rabbits, as evidenced by a prolonged anesthetic period, and good cardiovascular and other clinical indices. [ABSTRACT FROM AUTHOR]

Pseudomonas aeruginosa is one of the most important opportunistic pathogens in humans and animals. Natural antibacterial compounds like propolis are the best substitute for antibiotics. The aim of this study was to evaluate the antibacterial effect of ethanolic extract of propolis, propolis nanoemulsion, and their combinations with ciprofloxacin in the treatment of experimental wound infection contaminated with P. aeruginosa in rabbits. Propolis was obtained from a different region of Western Azerbaijan in the year 2018. Then the ethanol extract of propolis was prepared. And so, nanoemulsion of propolis was prepared. The broth microdilution method was used to determine the MIC of propolis and propolis nanoemulsion on P. aeruginosa. 28 rabbits used in 7 groups: Negative control (CO), Tween 20 (T), extract of propolis (P), nanoemulsion of propolis (NP), ciprofloxacin (C), ciprofloxacin+ extract of propolis (C + P), ciprofloxacin + nanoemulsion of propolis (C + NP). Full-thickness skin wound was created under general anesthesia and bacterial suspension (108 CFU/ml) was inoculated to each wound site. Macroscopic and microscopic characteristics and Superficial bacterial load of wounds were studied on days 7, 14, and 21. The number of bacteria in treatment groups was significantly lower than in negative control groups (p < 0.05). The macroscopic evaluation of wounds showed that C+P and C+NP enhanced wound closure in comparison with the negative control group and ciprofloxacin (p < 0.05). Histopathology assessment of the wound showed that the combination of C+P, and C+NP had a better and faster healing effect than the other groups, however, its difference was significant only when compared to CO, C, and T groups (p < 0.05). The results of the present study showed that a combination of ciprofloxacin + ethanolic extract of propolis and ciprofloxacin + propolis nanoemulsion had better therapeutic effects than either agent alone. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To observe the effect of Gubi Powder, an in-hospital prescription, on inflammatory factors and Notch3/Hes1 and Wnt/β-catenin signaling pathways in rabbits with knee osteoarthritis model, providing new drug ideas for clinical treatment. METHODS: Forty New Zealand rabbits were divided into sham operation group, model group, positive group (diclofenac potassium gel) and Gubi Powder group, with 10 rabbits in each group. The modified Hulth method was used to construct the rabbit knee osteoarthritis model, which lasted for 8 weeks. After modeling, normal saline was applied to the knee joints of the sham operation group and model group, diclofenac potassium gel was applied to the positive group, and Gubi Powder was applied to the Gubi Powder group, 8 hours a day, for 1 week. After administration, animal activity and behaviors were evaluated and scored; serum levels of prostaglandin E2, cyclooxygenase-2, interleukin 1β and tumor necrosis factor α were determined by ELISA; pathological changes of cartilage tissue were observed by hematoxylin-eosin staining and Safranin-O staining; protein levels of matrix metalloproteinases 1, 2, 3, and 13 in cartilage tissue of the knee joint were determined by immunohistochemistry; and protein levels of Notch3, Hes1, Wnt5a, β-catenin, Bcl-2, Bax, and Caspase-3 in cartilage tissue of the knee joint were determined by western blot assay. RESULTS AND CONCLUSION: Compared with the sham operation group, animals in the model group had difficulties in walking, serious cartilage injury in the knee joint, significantly elevated levels of serum prostaglandin E2, cyclooxygenase 2, interleukin 1β and tumor necrosis factor α (P < 0.05), significantly increased protein expression levels of matrix metalloproteinases 1, 2, 3, and 13, Bax, Caspase-3, Wnt5a and β-catenin in cartilage tissue (P < 0.05), and decreased protein expression levels of Notch3, Hes1, and Bcl-2 (P < 0.05). Compared with the model group, the walking condition of animals in the positive group and Gubi Powder group was improved, the serum levels of prostaglandin E2, cyclooxygenase-2, interleukin 1β and tumor necrosis factor α were decreased, cartilage damage of the knee was alleviated, the protein expression levels of matrix metalloproteinases 1, 2, 3, and 13, Bax, Caspase-3, Wnt5a and β-catenin in cartilage tissue were reduced (P < 0.05), and the protein expression levels of Notch3, Hes1, and Bcl-2 were increased (P < 0.05). To conclude, Gubi Powder can alleviate the symptoms of knee osteoarthritis in rabbits, improve the pathological changes of the cartilage, and reduce inflammatory factor levels in serum. The mechanism of action may be related to the inhibition of Notch3/Hes1 and Wnt/β-catenin signaling pathways. [ABSTRACT FROM AUTHOR]

Hair follicle (HF) undergo periodic growth and development in mammals, which regulated by dermal papilla cells (DPCs) are reported to play an important role in HF morphogenesis and development. However, primary DPCs have low proliferative activity, age quickly, and fresh cell isolation is both time-consuming and laborious. In this study, we introduced the SV40 large T antigen (SV40T) into dissociated early passage rabbit vibrissae DPCs with lentiviral vectors and established seven immortalized DPC lines (R-1, R-2, R-3, R-4, R-5, R-6 and R-7). These cell lines displayed early passage morphology and high alkaline phosphatase activity. RT-PCR and immunofluorescence staining showed that all the immortalized cell lines expressed the DPC markers (α-SMA, IGF1, ALPL, FGF2, BMP2 and TGFβ2), but α-SMA was only expressed well in R-3, R-4, and R-7. Furthermore, it was found that R-7 was the only line to survive beyond 50 passages. Compared to melanoma cells, R-7 did not undergo malignant transformation. Karyotyping and cell growth viability analysis illustrated that the R-7 cell line preserved the basic characteristics of primary DPCs. The R-7 DPCs established have potential application for future hair research. The study provides the theoretical basis in the cell research of HF growth and development. [ABSTRACT FROM AUTHOR]

In a simple randomized design trial, 420 growing male V-Line rabbits were randomly distributed into four groups to investigate the impact of exogenous dietary lysozyme on some physiological and nutritional parameters of male growing rabbits supplemented with exogenous dietary lysozyme. The witness group received a basal diet without exogenous dietary lysozyme (LYZ0), while the exogenous dietary lysozyme groups received 50, 100 and 150 mg/kg of basal diet (Groups; LYZ50, LYZ100 and LYZ150), respectively. The results showed significantly increased in blood cell count, hemoglobin concentration, total white blood cell, lipase, protease, amylase, total protein, triiodothyronine and thyroxine levels, while thyroid stimulating hormone levels significantly lessened in rabbits received LYZ. The LYZ- rabbit diets improved total digestible nutrient, digestible crude protein, and digestible energy values, with the LYZ100 group outperforming the others. LYZ-treated rabbits had significantly higher nitrogen intake, digestible nitrogen, and nitrogen balance than the witness group. The lysozyme in a rabbit's diet is taking on a new role as a digestive enzyme, enhancement thyroid hormones, as well as improvement hematology, daily protein efficiency ratio, daily performance index, hot carcass, total edible parts, nutritional value, and nitrogen balance, with decreasing the daily caloric conversion ratio and total non-edible parts. [ABSTRACT FROM AUTHOR]

Effects of dietary inclusion of spirulina platensis (SP) and selenium nanoparticles (SeNPs) combination (SP-SeNPs) on the reproductive performance in vivo and in vitro, reproductive and metabolic hormones, hemato-bichemical parameters, oxidative stress, and immunity of heat-stressed doe rabbis were evaluated. All supplements significantly increased live litter size at birth and weaning, viability rate at birth, hemoglobin and red blood cells, and plasma T3, T4, insulin, total proteins and albumin compared with control. Plasma estradiol 17-β (pre-mating), progesterone (mid-pregnancy), and prolactin (day −7 postpartum) were significantly increased only by SeNPs (0.3, 0.4, and 0.5 mg/kg). All dietary supplements significantly reduced WBCs, cortisol, lipid profile, and improved liver and kidney functions. Immunoglobulins levels, antioxidants capacity were significantly increased, superoxide dismutase was increased by SeNPs (0.4 and 0.5 mg/kg), while malondialdehyde was reduced by 0.3, 0.4 and 0.5 SeNPs mg/kg. Sexual receptivity, pregnancy rate, viability rate at weaning, ovulation rate, and embryo quality were significantly increased by increasing SeNPs above 0.1 mg, while embryo yield was increased by >0.2 mg SeNPs/kg. A combination of SP and SeNPs, could be potentially used as a strong antioxidant to enhance heat regulation and doe rabbit reproduction via improving reproductive and metabolic hormones, antioxidant status and immunological parameters. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To report the prevalence and document the treatment and outcome of odontogenic abscesses and associated jaw osteomyelitis in pet rabbits. ANIMALS: 200 client-owned rabbits. METHODS: Pet rabbits surgically treated using extraoral teeth extraction with marsupialization for abscesses of dental origin and jaw osteomyelitis were included in the study (February 2018 to February 2023). A case must have had pre- and postoperative computed tomographic study and a follow-up period of at least 4 months. RESULTS: In total, 113 male and 87 female rabbits were treated surgically. The mean age at the time of the diagnosis at the authors' clinic was 3 years and 11 months. Male rabbits suffered from odontogenic abscesses significantly more often than females. The mean surgical time varied from 25 to 95 minutes, based on theseverity of the pathologyand location. The wound healed completely in an average of 39.7 days (range, 14 to 145 days; 95% Cl, 36.9 to 42.5 days). Major complications detected in 18.5% (37/200) cases were associated with prolonged healing time mostly due to the formation of a bone sequestrum and gingival suture failure. The disease-free time following abscess resolution was on average 29 months (range, 4 to 60 months). The recurrence of the odontogenic infection was 8% (16/200 cases). CLINICAL RELEVANCE: The radical surgical technique with the extraction of all the infected teeth with the removal of all affected tissue and osteomyelitic bone and regular follow-up wound management is an effective method for the treatment of odontogenie abscesses with jaw osteomyelitis. [ABSTRACT FROM AUTHOR]

Augmentation rhinoplasty sometimes is required for patients with saddle nose deformity caused by failed rhinoplasty or facial trauma; finding appropriate grafting material remains a significant problem for this procedure. We investigated hyaluronic acid matrix as an allograft for dorsal augmentation rhinoplasty in a rabbit model. We performed an osteotomy on the nasal bones of eight rabbits. Four animals were sham operated as the control group and four were administered a mixture of saline-gelled hyaluronic acid matrix and sliced cartilage. Ultrasonography and three-dimensional reconstruction tomography were performed at the end of the experimental period. After sacrifice of the animals, nasal tissues were examined for histopathology, and both collagen scores and number of capillaries were compared between the two groups. Increased collagen and capillaries were apparent in the hyaluronic acid matrix group compared to controls. The median collagen score was significantly greater for the hyaluronic acid matrix group than for the control group. Although the number of capillaries for the hyaluronic acid matrix group was greater than for the control group, the difference was not statistically significant. Three weeks is sufficient for adhesion of ends of fractures in clinical practice; however, we found no ossification at this time in either group. A hyaluronic acid matrix may be a useful alternative supplement for dorsal augmentation rhinoplasty. Development of collagen was commensurate with membranous ossification; however, assessment of complete ossification requires a longer experimental period. [ABSTRACT FROM AUTHOR]

This study was conducted to evaluate the impact of dried moringa leaves (DML) on semen quality, immunoglobulin measurements, blood metabolic analysis, antioxidant status and sex hormones of V-line rabbit bucks. Twenty-four mature rabbit bucks of 7-8 months-old of proven fertility with an average weight (2.8 ± 0.4 kg) were classified into four treatments (basal diet with 0, 750, 1500 and 3000 mg DML/kg diet) and 6 bucks per treatment were used. Seminal plasma cholesterol (p = 0.013) and triglycerides (p = 0.0001) of all moringa supplemented rabbits were higher than the control. Moringa leaves enhanced (p < 0.05) serum testosterone, follicle stimulating hormone and luteinizing hormone. Total antioxidant capacity, superoxide oxidase, glutathione peroxidase and catalase in seminal plasma of moringa rabbits were higher (p < 0.05) than the control. Serum total lipid (p = 0.002), cholesterol (p = 0.008), triglycerides (p = 0.019) and blood urea (p < 0.05) were lower in DML rabbits. Moringa leaves improved total antioxidant capacity (p = 0.0001), glutathione peroxidase (p = 0.0001) and superoxide oxidase (p = 0.037) in rabbits versus control. Rabbits consuming DML had higher (p < 0.05) immunoglobulin IgG and IgM than control. Results suggest that DML improved rabbits' antioxidant, and immunological status, semen quality, and sex hormones, but DML (750 mg/kg diet) is recommended because it was more effective than the other two high doses. [ABSTRACT FROM AUTHOR]

N6-methyladenosine (m6A), the most abundant internal mRNA modification in eukaryotes, plays a vital role in regulating adipogenesis. However, its underlying mechanism remains largely unknown. Our previous study found that ADRB1 gene has m6A modification in both muscle and fat tissue. In this study, we interfered with FTO and ADRB1 genes After we cultured rabbit preadipocytes respectively. Oil red O staining and triglyceride assay were used to detect adipocyte differentiation. RT-qPCR was used to detect gene expression level and MeRIP-qPCR was used to detect the m6A modification level of gene. The results showed that FTO promoted the differentiation of adipocytes. At the same time, FTO up regulated the expression of ADRB1 gene and down regulated the m6A modification level of ADRB1 gene. Finally, we found that ADRB1 inhibited adipocyte differentiation. Together, we showed that FTO promoted adipocyte differentiation by regulating ADRB1 gene through m6A modification. [ABSTRACT FROM AUTHOR]

Severe heat stress is recognized as a factor that severely influences the semen quality and antioxidant status of rabbits. In this context, fresh onion juice (FOJ) may be a safe and useful option to improve reproductive performance. This research was designed to evaluate the anti-stress effects of FOJ on physiological and semen parameters in heat-stressed bucks. Thirty-six V-line rabbit bucks were randomly distributed into three groups. The bucks received FOJ orally at different doses [0 (water), 1.5, and 3 ml/kg live body weight] every 2 days over a period of 2 months, with 3 weeks as an adaptation period. FOJ treatments significantly improved semen characteristics, such as libido, mass and individual sperm motility, semen concentration, sperm viability, and acrosome reaction with increased initial seminal fructose, via the oral administration of 1.5 and 3 mL FOJ/kg body weight, compared with the findings in control bucks. Seminal plasma antioxidant status was significantly enhanced by FOJ treatments. It was concluded that the oral administration of FOJ under severe heat stress can improve bucks' semen characteristics and sex hormone concentrations except for testosterone, and it is considered a good strategy for improving the heat resistance of rabbit bucks, possibly due to its antioxidant activity. [ABSTRACT FROM AUTHOR]

Human respiratory syncytial virus (hRSV) is one of the major contagious viruses and causes complicated respiratory issues, especially in young children. The sensitive and fast detection of hRSV is critical for taking the most effective actions. In the present study, rabbit antibodies against the hRSV nucleoprotein (NP) were developed using phage display technology. A female rabbit was immunized with an hRSV strain A2 recombinant NP. A Fab library was built and sorted during two successive panning rounds for strain B and the A2 NP (recombinant preparations), respectively. The choice of candidates was performed using ELISA on the two NP strains. The obtained library was 3 × 106 cfu/mL, with an insertion rate of >95%. The two panning rounds permitted an enrichment factor of 100. ELISA screening allowed us to obtain 28 NP-specific Fab candidates. Among them, 10 retained candidates were reformatted into rabbit full IgG; thereafter, pairing tests on the recombinant strains and native lysate samples were performed. After the pairing tests on the recombinant strains, 53 pairs were identified. Eleven pairs were identified as being able to detect RSVs from native lysates. This work presents new high-potential monoclonal antibodies mAbs (mAbs), which would benefit from lateral flow testing data with patient materials. [ABSTRACT FROM AUTHOR]

A meta-analysis was conducted to determine the effects of probiotics on the performance, blood parameters, nutrient digestibility and carcase quality of domesticated rabbits. A dataset was constructed based on relevant published papers. An algorithm was constructed from 2004 to 2022, with a search in Scopus, Web of Science, Pub Med, and Medline using the MESH terms 'probiotics', 'rabbit', 'performance', 'blood parameters', 'nutrient digestibility', and 'carcasses'. After carefully evaluation, the final dataset consisted 35 in-vivo studies comprising 964 treatment units. The data analysis and coding were performed using software R version 4.2.1 'Funny-looking kid' computing with library mode (cowplot); (tidyverse); and (viridis); and (nlme). The results showed the level of probiotics increased body-weight gain with a linear pattern (p < 0.001). With regard to blood parameters, probiotics decreased triglycerides (p < 0.001) and concomitantly decreased albumin (p < 0.01) in domesticated rabbits. In conclusion, probiotics positively affect parameters for production performance and blood metabolites in domesticated rabbits. Probiotics helps to increased body-weight gain, suppressed triglycerides and albumin Elevated to carcase yield after exposure to probiotics Probiotic showed protective effects and enhanced immune systems in domesticated rabbit [ABSTRACT FROM AUTHOR]

The aim of the present research is to investigate breeders' management strategies and their effects on reproductive performances in heritage breeds. A total of 468 litters were analysed: Belgian Hare (N = 151), Burgundy Fawn (N = 105), Californian (N = 212). Litter size (born alive), percentage of weaned kits and percentage of selected kits were analysed using a model that included the effects of breed, year of birth, litter's origin (with three levels: both internal parents = 0, one outside parent = 1 both outside parents = 2) and their interactions (breed*year of birth, breed*litter's origin, year of birth*litter's origin, breed*year of birth*litter's origin). Besides, number of litters per buck and number of litters per doe were analysed using a model with only effect of breed. All these analyses were carried out with SPSS software. No effects of breeds on litter size, percentage of weaned and percentage of selected kits were found. Year of birth presented significant effects on litter size and percentage of weaned kits (p ≤ 0.05). The inclusion of external breeders didn't significantly influence analysed reproductive data. The number of litters per buck and per doe were not influenced by breed. The frequency of external breeders' inclusion is breed specific. Data recording is a basic step in phenotypical characterization, our results supply some information about breeders' management strategies and their effects on some reproductive parameters. Our results show breed specific frequencies of inclusions of external breeders and a positive effect of management procedures (genetic plus environment) on percentage of weaned kits according to the year of birth. Breeders management and reproductive parameters have been described The most frequent combination is a home bred doe mated to an external male A mean of 2 litters per doe and per buck have calculated in all the breeds The frequency of use of external breeders is breed specific [ABSTRACT FROM AUTHOR]

Bone substitutes are biomaterials applied in surgical therapy to repair many cases of a damaged bone. More than two million bone surgeries using biomaterials are realised each year worldwide. In this line of research, the researchers of this project study developed a bioresorbable synthetic bone substitute designated by (β-TCP/DCPD)-PHBV which is a mixture of the two materials: β-TCP/DCPD and PHBV in a 60/40 weight fraction. This research study attempts to categorize the (β-TCP/DCPD)-PHBV and evaluate its ability on triggering bone regeneration by making bone implants on 12 New Zealand white male rabbits. The RESEARCHERS performed multi-scale studies namely; a uniaxial compression test, an examination by scanning electron microscopy, and analysis with infrared spectroscopy in order to get the characteristics. The neoformed bone is examined by SEM coupled with energy dispersive spectroscopy (EDS). In addition to the salient mechanical benefits; attenuation of the modulus of elasticity and limitation of brittleness, the biomaterial has also proven to be effective in giving rise to new bone tissues having a suitable mineral composition. [ABSTRACT FROM AUTHOR]

Among the currently available commercial eye drops with cyclosporine A (Cs) there is a lack of long-acting dosage forms and products with a concentration of the drug substance higher than 0.1%, although Cs is widely used in ophthalmology. The aim of the research was to conduct the microscopic and biopharmaceutical evaluation of two formulations, an emulsion (EM) and a self-emulsifying oil (SEO), both with 0.5% of Cs, proposed for use in eye drops, and the comparison of both. SEO eye drops with Cs or any other drug substance are currently not available as marketed products, and the highest concentration of Cs in the ocular emulsion is only 0.1%. The microscopic evaluation of the emulsion and the SEO after emulsification with water was carried out using a high-resolution digital microscopy. The properties of both preparations were compared using the high dynamic range function or optical shadow effect mode. Images in the 3D composition mode were also recorded. The in vivo study of the Cs formulations was performed on male albino rabbits. The eye tolerance of the preparations was assessed using the ocular irritation test, which is a modified Draize test. Placebo carriers (without the drug substance) were also subjected to irritation testing. The concentration of Cs in the tissues (cornea and conjunctiva) and fluids (tear fluid and aqueous humor) of the rabbit eye was determined after multiple instillations of Cs–EM or Cs–SEO. The tested preparations were compared using the digital microscopy technique, which highlights the features of the formulations and eliminates the risk of unnoticeable properties that are difficult to observe in classical optical microscopy. Both tested Cs-loaded formulations are classified as practically non-irritating. There were also no significant differences when testing the placebo carriers. After a topical administration, Cs was widely distributed in all tissues (e.g., in cornea 1.3 ng/mg and 1.0 ng/mg) and fluids of the eye (e.g., in tear fluid 11.6 µg/mL and 4.3 µg/mL), after the administration of Cs–SEO and Cs–EM, respectively. The obtained results allow us to recognize both tested formulations, the emulsion and the self-emulsifying oil with 0.5% Cs content, as carriers safe for ophthalmic use and effective in delivering the drug substance to the structures of the eye. [ABSTRACT FROM AUTHOR]

Objective　To study the effects of different types of fluid resuscitation on mesenteric microcirculation and inflammatory factors in rabbits with hemorrhagic shock. Methods　The model of hemorrhagic shock rabbits was established by reducing the basic mean arterial pressure by 40% through draining the blood from the common carotid artery. Animals were randomly divided into control group，saline group，lactate Ringer group， acetic acid Ringer group， hydroxyethyl starch group and succinyl gelatin group with 8 animals in each group. Mesenteric microcirculation was monitored with microcirculation monitor. Mean arterial pressure（ MAP）， heart rate（HR），microvascular perfusion ratio（PPV） and microvascular blood flow index（MFI） were recorded before bleeding（T0），at hemorrhagic shock（T1），at the beginning of fluid resuscitation（T2），at the completion of fluid resuscitation（T3），and at the end of the experiment（T4） . The contents of tumor necrosis factor-α（TNF-α）， interleukin-1（IL-1） and lactic acid（Lac） were measured at T0，T2 and T4. Results　Compared with hydroxyethyl starch group，there were statistically significant differences in T3 MAP（P < 0.05），except for succinyl gelatin group， hydroxyethyl starch group had higher MAP at T4 than other groups，the difference was statistically significant（P < 0.05）. The differences in MAP between experimental control group and other groups were statistically significant at T4 （P < 0.05） . PPV and MFI of hydroxyethyl starch group and succinyl gelatin group were higher than those of normal saline group，lactic acid Ringer group and acetic acid Ringer group at T4（P < 0.05），and the lactic acid value of hydroxyethyl starch group at T4 was the lowest，compared with lactic acid Ringer group and normal saline group，the difference was statistically significant（P < 0.05）. There were statistical significances between all groups and experimental control group at T4 （P < 0.05）. There were no significant differences in TNF-αand IL-1 in T0， T2 and T4 among all groups（P < 0.05）. Conclusion　Hydroxyethyl starch solution and succinyl gelatin solution can improve the microcirculation of rabbits with hemorrhagic shock， but can not improve the level of inflammatory factors. [ABSTRACT FROM AUTHOR]

The present study aimed to investigate the effect of emulsifier lysophospholipids (LP), enzymatically modified from soy phospholipids, on the growth performance, nutrient digestibility, lipid metabolism and meat quality of fattening rabbits. The LP was added in control (CON), LP1, LP2 and LP3 at 0, 200, 400 and 600 mg/kg, respectively. A total of 240 rabbits at approximately 52 d of age were divided into 4 groups with 6 replicates of 10 rabbits each. The feeding trial lasted for 42 d. Results showed that compared to CON, LP1, LP2 and LP3 increased (p < 0.05) body weight gain, feed efficiency, the apparent faecal digestibility of gross energy, crude protein and ether extract, the percentages of dissectible fat and ether extract in the longissimus and legs, the serum contents of apolipoprotein B, free fatty acid and total phospholipids in the longissimus, but decreased (p < 0.05) serum total triglyceride and total cholesterol. Meanwhile, LP1, LP2 and LP3 had higher (p < 0.05) carcass weight, longissimus weight and percentages of foreleg and hindleg than the CON; and the three LP diets also increased (p < 0.05) the tenderness, lightness and redness of longissimus. It is concluded that soy LP as an emulsifier can improve the growth, digestibility and meat quality of fattening rabbits. [ABSTRACT FROM AUTHOR]

Simple Summary: Seasonal parameters, especially temperature and humidity, are reasonably speculated to affect livestock production performance. Among farmed animals, rabbits in particular are sensitive to extreme environmental temperature and humidity. This is not conducive to the performance of rabbit production and the improvement of product quality. Thus, a better understanding of the effects of the seasons on rabbits' physiology is fundamental to ensuring rabbit welfare, production efficiency, and fur quality improvement. Our study proved that the skinning season has a significant influence on the fur quality and hair follicle traits of Rex rabbits. The skin quality of rabbits slaughtered in winter was the best. Seasons may regulate hair follicle development via the Wnt10b/β-catenin, TGFβ-BMP, IGF1, and EGF signaling pathways in Rex rabbits. This experiment was conducted to study the effects of different skinning seasons on the fur quality and hair follicle development of Rex rabbits. A total of 80,150-day-old Rex rabbits were slaughtered on 15 July 2022 (summer), 15 October 2022 (autumn), 15 January 2023 (winter), and 15 April 2023 (spring) in Shandong Province (10 males and 10 females in each season). The results show that the skin weight, skin area, skin thickness, and hair follicle density of the Rex rabbits (at 150 days of age) were lower in summer than in winter (p < 0.05). Moreover, the coat length was shorter in summer than in spring, autumn, and winter (p < 0.05). The shoulder fat weight, perirenal fat weight, and perigastric fat weight of the Rex rabbits in winter were higher than those in summer (p < 0.05). Furthermore, the leptin levels in serum were higher in winter than in summer in the Rex rabbits (p < 0.05). In terms of serum biochemistry, the glucose levels were higher in autumn and winter than in spring and summer (p < 0.05). The cholesterol, high-density lipoprotein cholesterol (HDL), and low-density lipoprotein cholesterol (LDL) in summer had higher values than in winter in the Rex rabbits (p < 0.05). In winter, the expression of the Wnt10b, catenin beta 1 (CTNNB1), glycogen synthase kinase 3 beta (GSK3β), insulin like growth factor I (IGF-I), Type I insulin-like growth factor receptor (IGF-IR), and epidermal growth factor (EGF) genes was higher (p < 0.05), and the expression of the dickkopf-1 (DDK1), transforming growth factor beta 1 (TGFβ-1), bone morphogenetic protein 2 (BMP2), and bone morphogenetic protein 4 (BMP4) genes was lower than in summer (p < 0.05). In summer, the heat shock 70 kDa protein (HSP70) expression and CTNNB1 protein phosphorylation levels in skin tissue were higher than in spring, autumn, and winter (p < 0.05). In winter, Wnt10b protein expression was higher (p < 0.05), and GSK-3β protein phosphorylation levels were lower than in spring, autumn, and winter (p < 0.05). These results show that the skinning season can affect the production performance and hair follicle development of Rex rabbits. Compared with other seasons, the quality of skin from rabbits slaughtered in winter is better. Seasons may regulate hair follicle development via the Wnt10b/β-catenin, TGFβ-BMP, IGF1, and EGF signaling pathways in Rex rabbits. [ABSTRACT FROM AUTHOR]

Simple Summary: There is evidence that the genetic progress in paternal rabbit lines is lower than expected due to animal- and environmental-related factors, as well as founding factors intrinsically linked to the effectiveness of the selection process. In response to this question, we conducted a trial to evaluate the response after 18 generations of selection for increased growth rate within a paternal line on rabbit performance. Our results indicate that there were no differences in the key indicators in selection for growth rate (average daily gain and feed conversion ratio) between both populations differing in the generation of selection. These findings indicate a lack of effectiveness in the genetic progress of paternal rabbit lines based on different works carried out with these same genetic lines and generations. A total of 338 weaned rabbits (from the R line, selected for post-weaning growth rate) were used to evaluate the response to 18 generations of selection for increased growth rate on rabbit performance. Animals were obtained from two vitrified populations of the R line: R19V, belonging to the 18th generation (n = 165), and R37V, belonging to the 36th generation (n = 173), were allocated in individual and collective pens (178 and 160, respectively). A fattening trial was conducted from weaning (28 d of age until 63 d of age). During the trial, the body weight (BW), daily feed intake (DFI), average daily gain (ADG) and feed conversion ratio (FCR) were weekly monitored. Additionally, mortality and morbidity were daily registered. On days 49 to 53, an apparent faecal digestibility trial was also performed (12 animals per generation). Our results indicate that the generation of selection for growth rate did not affect mortality and morbidity. There were no differences in the diet digestibility according to the generation of selection. Regarding performance traits, R37V animals showed higher global BW (+6.7%; p = 0.0011) than R19V animals. R37V animals showed the same BW at weaning; however, R37V animals showed higher BW values in the last three weeks compared with R19V animals. Animals from the R37V generation also showed a higher DFI from 56 to 63 d of age (+12%; p = 0.0152) than R19V animals. However, there were no differences in global ADG and FCR between generations. These results indicate that the selection for growth rate in growing rabbits has slowed down, suggesting a lack of effectiveness in the genetic progress. [ABSTRACT FROM AUTHOR]

Keratoconus (KC) is characterized by localized, central thinning and cone-like protrusion of the cornea. Its precise etiology remains undetermined, although both genetic and environmental factors are known to contribute to disease susceptibility. Due to KC's complex nature, there is currently no ideal animal model to represent both the corneal phenotype and underlying pathophysiology. Attempts to establish a KC model have involved mice, rats, and rabbits, with some additional novel animals suggested. Genetic animal models have only been attempted in mice. Similarly, spontaneously occurring animal models for KC have only been discovered in mice. Models generated using chemical or environmental treatments have been attempted in mice, rats, and rabbits. Among several methods used to induce KC in animals, ultraviolet radiation exposure and treatment with collagenase are some of the most prevalent. There is a clear need for an experimental model animal to elucidate the underlying mechanisms behind the development and progression of keratoconus. An appropriate animal model could also aid in the development of treatments to slow or arrest the disorder. [ABSTRACT FROM AUTHOR]

The process of domestication of animals allowed for a change in the way of human life. Farm animals provided easy and relatively constant access to milk, meat, leather and wool. Farm animals are also increasingly used as pets and companions. Unfortunately, the appearance of animals in the human environment has also provided contact with new allergens. Allergy to fur animals has become a significant problem in the practice of an allergist. At present, it is a relatively common form of allergy and affects both adults and children. In the second part of the review of fur allergens, approved by the WHO/IUIS Subcommittee on allergen nomenclature, the allergens of rabbit, domestic cattle, horse, donkey, pig, goat and sheep were characterized. [ABSTRACT FROM AUTHOR]

Recent research in rabbit farming has focused on studying the impact of proper nutrition on rabbits during the growing stage. This is done in a proposal to find an effective strategy to reduce health problems in animals during weaning, while also considering the technical and economic aspects of this type of farming. Our study aims to clarify the effects of different types and quantities of dietary restriction on rabbits and their performance, based on various studies and research that have addressed this topic. Therefore, this research serves as a comprehensive summary of previous studies on dietary restriction in rabbits during the fattening stage. The results of these studies demonstrate the effectiveness of dietary restriction as a strategy, which can be adopted to achieve the desired economic goals of markets and consumers, as well as health-related objectives and overall profitability in rabbit farming projects. [ABSTRACT FROM AUTHOR]

Objectives] To observe the effect of Xianlingguhao Capsule on osteoporotic vertebral compression fracture (OVCF) in rabbits and the influence mechanism of the repair of fractures. [Methods] Female June age 30 rabbits were randomly divided into control group, model control group and Xianlinggubao group. After bilateral ovariectomy, the model control group and Xianlinggubao group were injected with dexa- methasone continuously for 4 weeks, and then the OVCF compound model was established by surgery. The Xianlinggubao group was treated with Xianlinggubao at a dose of 300 mg/(kg.d) for 60 d, while the blank control group and the model control group were treated with the same amount of normal saline for 60 d. The number of blood vessels and the expression of bone morphogenetic protein-2 (BMP-2) were detected by immunohistochemical staining and the bone mineral density (BMD) in the callus of the third lumbar fracture area of rabbits was meas ured. The content of serum phosphorus (P), alkaline phosphatase (ALP) and total calcium (TCa) in rabbit venous blood were measured by automatic biochemical analyzer. The content of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) in rabbit venous blood were measured by ELISA kit. [Results] The number of blood vessels and the expression of BMP-2 in the callus of the third lum- bar fracture area of rabbits was high in Xianlinggubao group, the content of serum P, ALP, TCa, VEGF and PDGF was obviously increased, BMD was obviously increased, the bone microstructure of the third lumbar vertebrae fracture area of rabbits was basically restored. Compared with the model control group (P<0.05), the difference was statistically significant. [Conclusions] Xianlinggubao Capsule can increase calcium and phosphorus deposition, promote the formation of blood vessels in the fracture area of OVCF in rabbits, and have a strong repair effect on OVCF in rabbits. [ABSTRACT FROM AUTHOR]

The aim of the study was to provide diagnostic imaging data on rabbit prostate complex by means of magnetic resonance imaging (MRI) with regard to their use in morphological investigations of the gland. Six anaesthetised sexually mature clinically healthy New Zealand White rabbits, 8 months of age, with body weight 2.8x3.2 kg were used. The pelvic cavity and pelvic organs were investigated in the sagittal, dorsal and transversal planes from the 7th lumbar (L7) to the 1st coccygeal (C1) vertebra with 2-mm slice thickness using a Magnetom Essenza 1.5T tunnel MRI scanner. The prostate complex (proprostate, prostate and paraprostate parts) demonstrated signal hyperintensity on T1-weighted MRI images. The prostate capsule was hyperintense compared to glandular parenchyma. The prostate complex was visualised on slices between the caudal part of the first sacral and cranial part of the third sacral vertebrae. The prostate part signal was hyperintense on T2-weighted MRI images. The shape of the prostate complex in dorsal view was craniocaudally elongated, oval and localised bilaterally from prostatic urethra in ventrodorsal view. The transverse T2 image of the pelvis through the 2nd sacral vertebra delineated the prostate part of the gland as a bilateral bilobed oval structure with hypointense parenchyma, embraced by a hyperintense capsule. The intrapelvic localisation and the shape of MRI image of healthy rabbit prostate complex is a species-specific morphological feature. The signal hyperintensity of rabbit prostate complex was higher on T2-weighted images. The T2 hyperintensity of prostate part vs hypointensity of proprostate and paraprostate parts provide evidence for the presence of substantial amount of glandular elements, presuming a dominating role of prostate part in the secretory function of the glandular complex. [ABSTRACT FROM AUTHOR]

Simple Summary: Computed tomography (CT) is a diagnostic imaging method frequently used for studying the heads of rabbits and guinea pigs, and dental pathologies are a common reason for requesting head CT examinations in these species. This study aimed to identify and characterize dental-related lesions in both species. All dental abnormalities were associated with secondary alteration of the perialveolar bone in rabbits and guinea pigs. The involvement of mandibular teeth was prevalent in both rabbits (81.2%) and guinea pigs (98%), compared to the maxilla. In the maxilla, increased nasal cavity attenuation (rhinitis) was the most frequent abnormality, observed in 60% of rabbits and 83.3% of guinea pigs; moreover, the exophthalmos was more frequent in rabbits (53.3%) compared to guinea pigs. In the mandible, cavernous space-occupying lesions were predominant in both rabbits (92.3%) and guinea pigs (73.3%). Dental issues often lead to secondary lesions of adjacent anatomical structures in both species, and CT examinations proved valuable for detecting these alterations. (1) Background: dental pathologies are the most frequent reason for requesting a CT scan of the head in rabbits and guinea pigs. The study aimed to review head CT exams of both species to identify and characterize lesions secondary to dental disease. (2) Methods: head CT studies of 48 rabbits and 52 guinea pigs with dental pathologies were reviewed. (3) Results: dental abnormalities of mandibular teeth were the most represented, both in rabbits (81.2%) and guinea pigs (98%). The aggressive bone lesion associated with teeth was the more frequently observed bone lesion in rabbits' mandible and maxilla; in guinea pigs, the more frequent bone lesions were bulging around the roots of the teeth with focal lysis in the maxilla, and without focal lysis in the mandible. In the maxilla, the increased attenuation of nasal cavities (rhinitis) was the most frequently observed abnormality both in rabbits (60%) and guinea pigs (83.3%); the exophthalmos was more represented in rabbits (53.3%). In the mandible, the cavernous space-occupying lesion was more represented both in rabbits (92.3%) and guinea pigs (73.3%). (4) Conclusions: lesions secondary to dental pathologies were often observed both in rabbits and guinea pigs; CT examination has proven to be valuable in detecting secondary alterations in both species. [ABSTRACT FROM AUTHOR]

A 2.5‐year‐old, 2.9 kg, neutered, male domestic rabbit presented for sudden onset of sneezing and wheezing of 2‐day duration. Upon physical examination, there was mild dyspnoea characterised by mild to moderate increased inspiratory effort and audible wheeze, without evidence of ocular or nasal discharge. An aggressive expansile lytic mass lesion of the right caudal maxilla extending into the zygomatic arch was identified on skull radiographs. Computed tomography confirmed a large heterogeneously contrast enhancing soft tissue mass associated with the lytic lesion, likely originating from the right alveolar bulla, extending into the zygomatic arch. Histopathological diagnosis was osteosarcoma with areas of osteogenic and fibroblastic patterns. Diseases of the nasal cavity are common in rabbits; while infectious rhinitis remains the most common diagnosis, increasing number of cases of neoplasms involving the maxilla and nasal cavity should remind clinicians to include it in the differential diagnosis list. [ABSTRACT FROM AUTHOR]

Indoxyl sulfates are uremic indolic toxins known to participate in the pathogenesis of cardiovascular diseases during chronic kidney disease in humans and some animal species. However, nothing is known about the indoxyl sulfate effect on the thyroid gland which is especially responsible for the general organism metabolism. This study determines the morpho-functional status of the thyroid gland after exposure to indoxyl sulfate (10, 25, and 50 mM) with the use of an ex vivo system and rabbit (n=10) as an experimental model thyroid gland histology, immunoexpression of thyrotropin receptor (TSHR), and concentrations of thyroxine (T4) and triiodothyronine (T3) were evaluated. Statistical analyses were performed using one-way analysis of the variance (ANOVA) followed by Tukey's post hoc comparison test. Minor alterations in thyroid tissue structure e.g. very rare exfoliated epithelial cells, condensed colloid fluid, or slight loosening of the epithelium were found. In addition, modulated dose dependent-expression of TSHR (p<0.01, p<0.001) together with a decreased level of T4 and T3 (p<0.001, p<0.01) exception of an increased level of T4 after the middle dose of indoxyl sulfate were revealed. We report here, for the first time, that indoxyl sulfate affects the thyroid gland mainly at the molecular level. The rabbit thyroid gland ex vivo system seems to be suitable for further studies on the thyroid gland in health and disease. However, the effect of TSH-TSHR signaling at ultrastructural, and epigenetic levels needs supplementary appraisal. [ABSTRACT FROM AUTHOR]

Objective: Biological data on the beneficial effects of vocal fold rehydration are lacking. This study aimed to examine the effects of acute systemic dehydration on vocal fold gene expression and determine whether rehydration would reverse these changes. Methods: Male New Zealand White rabbits (N = 24, n = 8/group) provided the animal model. Systemic dehydration was induced by 5 days of water volume restriction. Rehydration was provided by ad‐lib water for 3 days following dehydration. Euhydrated rabbits were used as the control group. Vocal fold tissue was dissected. Seventeen genes were selected based on physiological function and role in supporting vocal fold structure, oxidative stress, hemodynamics, and extracellular matrix turnover. Relative gene expression was assessed by RT‐qPCR. Results: Rehydration following systemic dehydration can modulate gene expression, with expression patterns suggesting that rehydration reverses dehydration‐induced changes in over half of the tested genes. CLIC5 (chloride intracellular channel 5) and EFEMP1 (EGF containing fibulin extracellular matrix protein 1) genes were significantly upregulated in the dehydration group compared with the euhydrated control. A1BG (alpha‐1B‐glycoprotein) and IL1RAP (interleukin 1 receptor accessory protein) were downregulated by rehydration compared with the dehydration group. Conclusion: This study provides molecular evidence for a transcriptional response to rehydration following acute systemic dehydration in the vocal folds. These data are the first to study gene expression following realistic dehydration and rehydration paradigms and provide biological data to support clinical recommendations to increase water intake after acute dehydration. Level of Evidence: NA Laryngoscope, 133:3499–3505, 2023 [ABSTRACT FROM AUTHOR]

This experiment aimed to investigate changes, in the structural-functional aspects of the pineal and pituitary glands as well as the ovary and uterus, that might associate with trypanosomiasis induced by T. evansi in female rabbit. The ovarian and uterine response to the luteinizing hormone-like activity (Human Chorionic Gonadotropin, HCG) was also tested. Trypanosomiasis did not cause significant changes in body, ovarian and uterine weights. There were degenerative changes in the structural-functional picture of the studied organs. T. evansi resulted in decreased response of the ovaries and uteri to HCG. The direct and indirect effects, including somatic and autoantibodies production, of this species of trypanosomes might be responsible for the reported findings.

ABSTRACTPasteurella multocida serogroup F can infect a number of animals. However, the pathogenicity and genomic features of this serogroup are still largely unknown. In the present study, the pathogenicity and genomic sequences of 19 rabbit-sourced P. multocida serogroup F isolates were determined. The 19 isolates were highly pathogenic for rabbits causing severe pathologic lesions and high mortality in inoculated rabbits. Nevertheless, the pathologic lesions in rabbits caused by the 19 isolates were distinct from those caused by the previously reported high-virulent serogroup F strains J-4103 (rabbit), P-4218 (turkey), and C21724H3km7 (chicken). Moreover, the 19 isolates were avirulent to white feather broilers. The genomes of the 19 isolates were determined to understand the pathogenicity of these isolates. The finding of a number of functional genes in the 19 isolates by comparison with the low-virulent rabbit-sourced serogroup F strain s4 might contribute to the high virulence of these isolates. Notably, polymorphisms were determined in the lipopolysaccharide outer core biosynthetic genes natC and gatF among the serogroup F strains of different hosts. However, the sequences of natC and gatF from rabbit-sourced strains (except for SD11) were identical, which might be responsible for the host specific of the 19 isolates. The observations and findings in this study would be helpful for the understanding of the pathogenicity variation and host predilection of P. multocida.IMPORTANCEThe 19 rabbit-sourced Pasteurella multocida serogroup F isolates showing high virulence to rabbits were avirulent to the broilers. Notably, polymorphisms were determined in the lipopolysaccharide outer core biosynthetic genes natC and gatF among all serogroup F strains of different hosts. However, the sequences of natC and gatF from rabbit-sourced strains (except for SD11) were identical, which might be responsible for the host specific of the 19 isolates.

Abstract Background Rabbits are extremely sensitive to pain. There is evidence on whether non‐steroidal anti‐inflammatory medication should be administered before or after surgery in rabbits. Objectives In this study, we compared the pain scores and behaviours of rabbits administered meloxicam before or after routine castration. Methods Forty‐two healthy rabbits undergoing castration were randomly assigned to receive meloxicam (1 mg/kg SC) 1 h before or upon recovery from anaesthesia. Several parameters were recorded, including the occurrence of eating, defecating and pain scores postoperatively and change in heart rate intraoperatively. Results At 4 h after surgery, eating was observed in 43% (9/21) of rabbits that received meloxicam before neutering and in 29% (6/21) of rabbits that received meloxicam after. The morning after the surgery, eating was observed in 62% (13/21) of rabbits that received meloxicam before neutering and in 57% (12/21) of rabbits that received meloxicam after. The odds of not eating increased by 1.16 times (1.03–1.29; p = 0.01) per month of age, whereas there was no significant association between eating and administration of meloxicam before or after surgery, body weight and time. Similarly, defecation, pain score and change in heart rate did not differ in animals receiving meloxicam before or after surgery. Conclusions Administration of meloxicam in rabbits undergoing castration before or after surgery does not have a relevant effect on eating, defecating or pain score. Younger rabbits show less obvious signs of pain and are more likely to resume eating habits sooner after surgery compared to older rabbits.

IntroductionHeat-killed probiotics, as a type of inactivated beneficial microorganisms, possess an extended shelf life and broader adaptability compared to their live counterparts. This study aimed to investigate the impact of heat-killed Lactobacillus acidophilus (L. acidophilus, LA) - a deactivated probiotic on the growth performance, digestibility, antioxidant status, immunity and cecal microbiota of rabbits.MethodsTwo hundred weaned Hyla rabbits were randomly allocated into five equal groups (CON, L200, L400, L600, and L800). Over a 28-day period, the rabbits were fed basal diets supplemented with 0, 200, 400, 600, and 800 mg/kg of heat-killed LA, respectively.ResultsResults revealed a significant reduction in the feed-to-gain ratio (F/G) in the L600 and L800 groups (p

Background and Aim: Fungal infections are a growing problem for both humans and animals due to the emergence of pathogenic strains resistant to modern antifungal treatments. To evaluate the efficacy of new antifungal drugs, it is essential to develop animal models that demonstrate typical responses to both the infection (pathogenesis and clinical course) and to the treatment, including adverse effects. In this study, we established a rabbit otitis model by infection of an aggressive multidrug-resistant strain from dogs, Malassezia pachydermatis C23, with no need for concomitant immunosuppression. Materials and Methods: Twenty healthy adult male gray giant rabbits (1 year old, 5.5 kg) were inoculated once with M. pachydermatis C23 at 108 colony-forming units/mL. We observed the clinical signs of the disease and collected ear smears and blood samples every 5 days. Results: The infection progressed rapidly and exhibited characteristic clinical signs without spontaneous recovery for at least 1 month. In fact, substantial deterioration was observed as evidenced by blood parameters. Conclusion: This rabbit otitis model established using an aggressive drug-resistant fungus strain without immunosuppression could prove valuable for testing novel antifungal agents.

Abstract Objective To explore the mechanism of the p38MAPK signaling pathway in repairing articular cartilage defects with biological collagen membranes. Methods Thirty-two healthy adult male rabbits were randomly divided into a control group (n = 8), model group (n = 8), treatment group (n = 8) and positive drug group (n = 8). The control group was fed normally, and the models of bilateral knee joint femoral cartilage defects were established in the other three groups. The knee cartilage defects in the model group were not treated, the biological collagen membrane was implanted in the treatment group, and glucosamine hydrochloride was intragastrically administered in the positive drug group. Twelve weeks after the operation, the repair of cartilage defects was evaluated by histological observation (HE staining and Masson staining), the degree of cartilage repair was quantitatively evaluated by the Mankin scoring system, the mRNA expression levels of p38MAPK, MMP1 and MMP13 were detected by real-time fluorescence quantitative PCR (qRT-PCR), and the protein expression levels of p38MAPK, p-p38MAPK, MMP1 and MMP13 were detected by Western blotting. The results after the construction of cartilage defects, histological staining showed that the articular cartilage wound was covered by a large capillary network, the cartilage tissue defect was serious, and a small amount of collagen fibers were formed around the wound, indicating the formation of a small amount of new bone tissue. In the treatment group and the positive drug group, the staining of cartilage matrix was uneven, the cytoplasmic staining was lighter, the chondrocytes became hypertrophic as a whole, the chondrocytes cloned and proliferated, some areas were nest-shaped, the cells were arranged disorderly, the density was uneven, and the nucleus was stained deeply. The Mankin score of the model group was significantly higher than that of the control group, while the Mankin scores of the treatment group and positive drug group were significantly lower than that of the model group. The results of qRT-PCR detection showed that compared with the control group, the expression level of the p38MAPK gene in the model group did not increase significantly, but the gene expression levels of MMP1 and MMP13 in the model group increased significantly, while the gene expression levels of MMP1 and MMP13 decreased significantly in the treatment group and positive drug group compared with the model group. The results of Western blot detection showed that compared with the control group, the expression level of p38MAPK protein in the model group was not significantly increased, but the phosphorylation level of p38MAPK protein and the protein expression levels of MMP1 and MMP13 were significantly increased in the model group, while the phosphorylation level of p38MAPK protein and the protein expression levels of MMP1 and MMP13 in the treatment group and positive drug group were significantly lower than those in the model group. Conclusion The biological collagen membrane can regulate the expression of MMP1 and MMP13 and repair the activity of chondrocytes by reducing the phosphorylation level of p38MAPK and inhibiting the activation of the p38MAPK signaling pathway, thus improving the repair effect of articular cartilage defects in rabbits. The P38MAPK signaling pathway is expected to become an important molecular target for the clinical treatment of cartilage defects in the future.

Background: The effects of post mortem interval on tryptase levels are unclear and have been challenging to quantify due to limited and at times contradictory research. Purpose: Analyze the effects of Post Mortem Interval (PMI) due to anaphylactic shock on the expression of β-tryptase and mast cell chymase in the heart organ. Method: This type of research was an experimental study with a randomized block design (RBD) with the same subjects and a time series for 24 hours, using 5 (five) rabbits. The measurement of β-tryptase and chymase expression was based on immunohistochemical IRS (immunoreactive score) in the heart organs of rabbits experiencing anaphylactic shock sensitization and induction of anaphylactic shock using ovalbumin. Result: Based on the results of statistical tests using univariate analysis, there was a relationship between the length of death (post mortem interval) and the expression of mast cells β-Tryptase and cardiac chymase (p-value = 0.006) and chymase (p-value = 0.002) with (p-value

The study aimed to evaluate the effectiveness of Gabapentin in controlling postoperative pain in New Zealand white rabbits after ovariohysterectomy. Twenty sexually mature and healthy female rabbits were divided into four groups: a negative control, Meloxicam treatment, Gabapentin treatment, and Meloxicam plus Gabapentin treatment. After the surgery, the rabbits" pain levels were assessed using the Grimace Scale at various time points. The Gabapentin group consistently had the lowest Grimace Scale scores. Statistical analysis showed significant differences between the Meloxicam group and the negative control, the Gabapentin plus Meloxicam group and the negative control, the Gabapentin group and the negative control, and the Gabapentin group and the Gabapentin plus Meloxicam group. Postoperative analgesia was significantly better in the Meloxicam, Gabapentin, or combined treatment groups compared to the negative control. Gabapentin was found to be equally effective as Meloxicam in controlling pain. However, the combination of Meloxicam and Gabapentin was not as effective as Gabapentin alone. In conclusion, Gabapentin showed preventive efficacy in controlling postoperative pain after ovariohysterectomy in New Zealand white rabbits. These findings suggest that Gabapentin could be a valuable analgesic option for surgeons to provide adequate pain control in this surgical context. Further research is needed to explore optimal dosing and potential synergistic effects when combining Gabapentin with other analgesics.

AIM: To improve the standard three-port vitrectomy for establishing and evaluating an endotamponade model in rabbits. METHODS: Three ports were prepared near the third eyelid of rabbits, and the infusion port was placed at the inferior nasal quadrant with the inserted cannula linking with a self-designed handheld rigid infusion catheter. All right eyes of rabbits underwent a modified 25-gauge vitrectomy and were subsequently filled with balanced salt solution, silicone oil, and eight-arm polyethylene glycols (8-arm PEGs) hydrogel separately for comparison. Ophthalmic examinations were performed regularly to record the changes after the surgery. RESULTS: Successful vitrectomy was achieved among 44 chinchilla rabbits. The mean operation time was 4.51±1.25min. Four eyes (9.1%) presented limited lens touch and two eyes (4.5%) showed retinal touch during surgery. Incision leakage was found in three eyes (6.8%) after surgery. There was no endophthalmitis, hemorrhage, or retinal detachment during the observation period and ophthalmic examinations after the implantation of vitreous substitutes. CONCLUSION: The modified technique of the standard vitrectomy applied in the endotamponade model in rabbits shows excellent safety and practicality.