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788 results on '"Pisegna, Joseph R."'

203. Cloning and Characterization of the Signal Transduction of Four Splice Variants of the Human Pituitary Adenylate Cyclase Activating Polypeptide Receptor: EVIDENCE FOR DUAL COUPLING TO ADENYLATE CYCLASE AND PHOSPHOLIPASE C

Author

Pisegna, Joseph R. and Wank, Stephen A.

Subjects
Inositol Phosphates, Molecular Sequence Data, Transfection, Polymerase Chain Reaction, Second Messenger Systems, Article, Protein Structure, Secondary, Mice, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, DNA Primers, Neurotransmitter Agents, Base Sequence, Neuropeptides, Genetic Variation, 3T3 Cells, Exons, Recombinant Proteins, Rats, Alternative Splicing, Kinetics, Pituitary Gland, Type C Phospholipases, Pituitary Adenylate Cyclase-Activating Polypeptide, Adenylyl Cyclases, Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I, Signal Transduction
Abstract

Alternative splicing of two exons of the rat pituitary adenylate cyclase activating polypeptide (PACAP) receptor gene generates four major splice variants that are differentially expressed in specific tissues and variably coupled to intracellular second messengers. To evaluate the potential implications of these findings in human physiology, the human PACAP receptor gene was cloned. Alternative splicing about two exons of the gene allowed for four major splice variants that were subsequently identified on cDNA cloning. Each of the four splice variant cDNAs (null, SV-1, SV-2, and SV-3) was stably expressed in NIH/3T3 cells at similar receptor densities. For each splice variant, PACAP (both PACAP-38 and PACAP-27) had similar affinity and potency for stimulating either adenylate cyclase or phospholipase C. However, each receptor splice variant differed in their ligand-stimulated maximal response (efficacy) for total inositol phosphate accumulation with the SV-2 showing the greatest efficacy, followed by the null, SV-1, and SV-3 splice variants. Therefore, unlike the rat, PACAP binds and stimulates signal transduction with nearly equal affinity and potency for each of the receptor splice variants although with varying efficacy for the stimulation of phospholipase C. These results suggest a novel and potentially important mechanism for a single hormone to not only couple to dual signal transduction cascades but also elicit tissue-specific differential activation of phospholipase C in humans.

Published
1996

206. Expression of gastrin in the thin descending limb of Henle's loop in the mouse kidney: a molecular, localization, and functional study

Author

Yang, Yu, primary, Evans, Sarah, additional, Escano, Crisanto, additional, Asico, Laureano, additional, Zhang, Yanrong, additional, Gonzalez, Santiago Cuevas, additional, Villar, Van Anthony, additional, Wang, Xiaoyan, additional, Pisegna, Joseph R., additional, Wank, Stephen A., additional, Armando, Ines, additional, and Jose, Pedro, additional

Published
2012
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217. Molecular Cloning, Functional Expression and Chromosomal Localization of the Human Cholecystokinin Type A Receptor

Author

Deweerth, A., Weerth, Andreas, Pisegna, Joseph R., Huppi, Konrad, and Wank, Stephen A.

Subjects
hormones, hormone substitutes, and hormone antagonists
Abstract

The cholecystokinin (CCK) family of peptides and receptors are present throughout the brain and gastrointestinal tract and can be pharmacologically subdivided into two subtypes: CCKA and CCKB. Little is known about the localization, pharmacology and function of CCKA receptors (CCKAR) in humans. We used the rat CCKAR cDNA to isolate the human CCK receptor cDNA homologue from human gallbladder which encodes a unique 428 amino acid protein having >90% homology to the rat and guinea pig CCKAR. Expression of the recombinant CCKAR in COS-7 cells displayed a pharmacological profile characteristic of a CCKAR subtype and mediated agonist stimulated increase in total inositol phosphates. Northern hybridization identified a transcript measuring 6 Kb. The human CCKAR gene maps to chromosome 4. These results describe for the first time the molecular cloning, expression and chromosomal localization of the human CCKA receptor.

Published
1993

220. Good Samaritan bad act?

Author

Cain, May L. and Pisegna, Joseph R.

Subjects
Assistance in emergencies -- Laws, regulations and rules
Published
1989

250. PACAP in enteric neurons of the rat stomach

Author

Sternini, Catia, primary, Pham, Thomas, additional, Wong, Helen E., additional, Miampamba, Marcel, additional, Pisegna, Joseph R., additional, Brecha, Nicholas C., additional, and Walsh, John H., additional

Published
2000
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