201. MEG3 polymorphisms associated with peripheral blood leukocyte mitochondrial DNA copy number in PAHs-exposure workers.
- Author
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Li X, Duan X, and Wang W
- Subjects
- Male, Humans, DNA, Mitochondrial genetics, DNA Copy Number Variations, Mitochondria genetics, Leukocytes chemistry, Polycyclic Aromatic Hydrocarbons toxicity, Polycyclic Aromatic Hydrocarbons analysis, Coke analysis, Occupational Exposure analysis
- Abstract
Background: Epidemiological studies have shown that exposure to Polycyclic aromatic hydrocarbons (PAHs) is associated with reduced mitochondrial DNA copy number (mtDNA-CN). Long non-coding RNA maternally expressed gene 3 (MEG3) is involved in mitochondrial function regulation. However, it is unknown whether single-nucleotide polymorphisms in the MEG3 can regulate the mtDNAcn in PAHs exposed populations. The aim of this study was to examine the effect of MEG3 genetic polymorphisms on the mtDNA-CN in PAHs exposed populations., Materials and Methods: We recruited 544 coke oven workers and 238 controls using random cluster sampling. High-performance liquid chromatography was used to detect the concentrations of four OH-PAHs (1-hydroxypyrene [1-OHPyr], 1-hydroxynathalene [1-OHNap], 2-hydroxynathalene [2-OHNap], and 3-hydroxyphenanthrene [3-OHPhe]) in urine. The mtDNA-CN of peripheral blood leukocytes was measured using the quantitative polymerase chain reaction method. Sequenom Mass ARRAY matrix-assisted laser desorption/ionization-time of flight mass spectrometry platform was used to detect ten polymorphisms in MEG3., Results: The OH-PAHs levels in the exposure group were significantly higher than those in the control group (P < 0.001). The mtDNA-CN in the exposure group was significantly lower than that in the control group (P < 0.001). A linear regression model revealed that PAHs-exposure (β [95% confidence interval, CI], -0.428 [-0.475, -0.381], P < 0.001), male gender (-0.052 [-0.098, -0.005], P = 0.029), genotype TT for MEG3 rs11859 (-0.088 [-0.142, -0.035], P = 0.001), and genotype GG for MEG3 rs7155428 (-0.114 [-0.210, -0.017], P = 0.021) were associated with decreased mtDNA-CN., Conclusion: PAHs-exposure, male gender, genotype TT for rs11859, and genotype GG for rs7155428 were risk factors for mtDNA-CN., Competing Interests: Declaration of competing interest All the authors declare that they have no conflicts of interest., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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