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The relationship between milk fat progesterone concentration at first artificial insemination (AI) and reproductive performance of Norwegian Red Cattle dairy cows was investigated in a field study. Fifteen AI technicians collected milk samples from 2250 cows from 458 herds and progesterone was analysed in the milk fat portion of the samples. Logistic regression revealed decreased probability of non-return, and increased likelihood of repeat-breeding, with increasing progesterone concentrations at insemination. The odds ratio for non-return and for repeat-breeding between the minimum and maximum value for milk fat concentrations between 4 and 32.2ng/ml was 4.5 (P0.01) and 3.2 (P = 0.01), respectively. The variable 'Technician' did not significantly influence non-return rate or repeat-breeding. Progesterone concentration at AI was positively related to calving to last insemination interval, and to number of inseminations per cow (P0.05). The association between the progesterone concentration at insemination and calving interval was only marginally significant. Technician was significantly (P0.01) associated with interval from calving to first AI, interval from calving to last AI (P = 0.05), and number of AI per cow (P = 0.01). Technician was not significantly related to the calving interval. We conclude that increased suprabasal progesterone concentrations at the time of first insemination, and higher return rate at AI may, at least partly, be due to endocrinological asynchrony at AI, a condition which may lead to decreased fertility.

Milk samples of 59 cows of the Norwegian Red Cattle breed receiving three different supplementary concentrates, were analysed for genotypes of caseins and whey proteins, the content of different milk salts (Ca 2+ , Ca, Mg and citrate), the content of total protein, casein and whey protein and the mean micellar size of native and heated casein micelles. The genotype of α s1 -casein had a statistically significant effect on the content of protein and casein, and the content of whey protein and the casein number were significantly influenced by different feeding regimes, and the content of citrate. The mean size of native and heated casein micelles was significantly influenced by the feeding regimes, genotype of α s1 -casein (native mean size only) and κ -casein, pH and the content of casein, whey protein and casein number. The heat-induced changes in mean micellar size were significantly affected by the calcium ion activity which accounted for approximately 40% of the total variation.

A Norwegian dairy herd was examined during and after conversion to ecological management and low concentrate feeding (maximum 3 FEm per day and cow) in 1989–1994. Records from 34 Norwegian Red cows representing 81 lactations were subjected to ANOVA. Mean annual milk yields of 4667 kg, 5478 kg and 6155 kg per cow during first, second and later lactations, respectively, were obtained after conversion. A reduction in number of cows and hence, total milk yield resulted from decreased feed import and reduced forage yields. Decreasing milk yield and increasing protein percentage was found during conversion when effects of calving season, lactation month and number, concentrate ration and cow were corrected for. However, years with large deviations from this trend were observed, demonstrating sensitivity to variations in the farm’s feed production. Periods with pasturing showed the highest milk yield and protein percentage for most seasons of calving, and hence spring calvers obtained the highest milk yield for the whole lactation. Lower somatic cell count in milk was also observed for cows calving in spring and summer compared to calving in autumn and winter. With respect to daily milk yield and protein percentage, cows with higher sire index for protein yield and protein percentage were found superior in this production system. Cows sired by high genetic merit bulls for protein yield also showed higher somatic cell counts than cows sired by low genetic merit bulls, but cows sired by bulls with high overall breeding value showed lower somatic cell counts.

This commentary deals briefly with various topics discussed in the recent annual course in Transfusion Medicine, organized by the Norwegian Red Cross Blood Programme.

4-methylimidazolc (4-MeI) has until now been the only identified toxic compound in ammoniated forage considered to be of possible etiological significance in ammoniated forage poisoning. However, several authors have concluded that 4-MeI alone cannot explain the toxicity observed (Morgan & Edwards 1986, Nielsen et al 1986, Motoi et at. 1997). On this background, we have examined samples of ammoniated forage and of milk, plasma, and urine collected from ewes and lambs during a previous experimental poisoning study (Sivertsen et al. 1993). We have also studied similar samples from a Norwegian Red Cattle dairy cow fed ammoniated hay for the first 5 days after calving. We have, in addition to the previously known ingredients 4-MeI and 2-methylimida-zole (2-MeI), identified 5 new compounds in these samples, all of which were found to be di-alkylsubstituted imidazoles: 1,2-dimcthylimi-dazole (1,2-diMeI), 1,4-dimethyl imidazole (1,4-diMeI), 1,5-dimethyl imidazole (1,5-diMeI), 2,4-dimethylimidazole (2,4-diMeI) and 2-ethyl, 4-methylimidazole (2Et-4MeI) (Müller et al. 1998). In this paper we present quantitative measurements of these di- and monoalky 1 imidazoles in a selection of the collected samples.

We present a model on plant-deer climate interactions developed for improving our understanding of the temporal dynamics of deer abundance and, in particular, how intrinsic (density-dependent) and extrinsic (plants, climate) factors influence these dynamics. The model was tested statistically by analysing the dynamics of five Norwegian red deer populations between 1964 and 1993. Direct and delayed density-dependence significantly influenced the development of the populations: delayed density-dependence primarily operated through female density, whereas direct density-dependence acted through both female and male densities. Furthermore, population dynamics of Norwegian red deer were significantly affected by climate (as measured by the global weather phenomenon, the North Atlantic Oscillation: NAO). Warm, snowy winters (high NAO) were associated with decreased deer abundance, whereas the delayed (two-year) effect of warm, snowy winters had a positive effect on deer abundance. Our analyses are argued to have profound implications for the general understanding of climate change and terrestrial ecosystem functioning.

Nine Norwegian Red cattle, aged 7-14 months, persistently infected with bovine virus diarrhoea virus (BVDV) were inoculated with a Swedish strain of Babesia divergens. Six persistently infected cattle of the same age and breed were kept as controls. Blood and serum samples were collected regularly during the observation period. Rectal temperatures were recorded every morning for 25 days post infection, and the animals were examined clinically on a daily basis. Sera were examined for antibodies to B. divergens by indirect immunofluorescence antibody test (IFAT). Eight of the infected animals developed fever of 2-5 days duration. Babesia divergens organisms appeared in the erythrocytes of all infected animals on the day after inoculation. The parasitaemia lasted for 4-11 days. One animal had a transient haemoglobinuria. Compared with the control group, there was a 20% decrease in the haematocrit. There was a transient lymphopenia and thrombocytopenia during the period of fever. There were no differences in mean numbers of neutrophils between the two persistently infected groups. Compared with cattle free of BVDV, the persistently infected cattle had consistently lower total leucocyte count that was mainly due to decreased mean numbers of neutrophils and monocytes. All infected animals develop antibodies > or = 1:1280 between day 7 and 10 post infection. The magnitude of the antibody response was considerably lower than that of BVDV-free animals inoculated with the same strain and dosage of B.divergens.

In the following pages I will give an account on how the Norwegian Red Cross reacted to the tragic killing of six ICRC delegates in Chechnya in December 1996. Then was the time for action; now is the time for reflection.For the Movement as a whole it is vital that we now focus on the security issue as it affects our Red Cross/Red Crescent workers. At the same time, we must never let evil acts like this paralyse our ability to operate in conflict areas.

SUMMARYIn a previous investigation, fiveSarcocystisspecies were described from Norwegian red deer and believed to be conspecific with species occurring in either reindeer or moose based on sarcocyst morphology and nucleotide sequences of the nuclear ribosomal DNA unit. The aim of the present study was to characterize numerous isolates of these sarcocyst types at the mitochondrial cytochrome c oxidase subunit I gene (cox1) in order to corroborate or refute previous species designations ofSarcocystisin red deer. TheSarcocystis tarandi- andSarcocystis rangiferi-like taxa in red deer and reindeer, respectively, were thoroughly compared by sequencing 14–27 isolates of each type. Sequence comparisons revealed four distinct sequence types, which by phylogenetic analyses were placed in four monophyletic groups according to host origin, and they were therefore considered to represent four separate species. The two taxa of this type in red deer were namedSarcocystis elongataandSarcocystis truncata, respectively. Sequencing of many isolates ofSarcocystis hjortiandSarcocystis ovalisfrom red deer and moose confirmed that these species occur in both hosts. A revised description of the two new species is given and the current knowledge concerning all sixSarcocystisspecies in red deer is reviewed.

Disinfecting footbaths are used to treat and prevent interdigital dermatitis (ID) and heel horn erosion (HHE). However, many disinfectants are disadvantageous for the environment and, as an alternative, washing of the feet has been introduced. Our aim was to investigate the effect of water footbaths (trial 1), footbaths with CuSO4 (trial 2), automatic water flushing (trial 3), and water flushing followed by disinfection with a glutaraldehyde-based compound (trial 4) in 4 randomized controlled clinical trials performed in a freestall dairy herd of approximately 45 Norwegian Red cows. At trimming before and after each trial, hind foot diseases, hardness of the claw horn (in D-units), locomotion, and cleanliness of the claws were recorded. Before each trial, the cows were divided in comparable study and control groups, based on prevalence of ID and HHE, parity, and days in milk. Using a transponder-regulated gate, the study groups were led through a footbath (trials 1 and 2) or an automatic washer (trials 3 and 4), whereas the control groups were left untreated. Each trial lasted 3 mo and the curative effect in diseased cows and the preventive effect in cows with healthy feet on ID, HHE, and ID + HHE were analyzed. In trial 2, a preventive effect of CuSO4 on HHE compared with the untreated cows was observed. During trial 1, 100% (11/11) of the treated cows with ID got better and 22% (2/9) without ID became diseased, whereas 92% (11/12) of the treated cows with ID + HHE got better and 38% (3/8) without ID + HHE became diseased. During trial 2, 69% (9/13) of the treated cows with ID got better and 11% (1/9) without ID became diseased. During trial 4, 19% (3/16) of the untreated cows with ID + HHE got better and 71% (5/7) without ID + HHE became diseased. In trial 3, no significant effects on ID, HHE, or ID + HHE were revealed. In trial 2 (CuSO4), the treated cows’ claw horn was harder after the trial compared with the controls (D-unit difference: 13.25). In trial 3 (stationary water flushing) the treated cows’ claw horn was softer after the trial when compared with the controls (D-unit difference: −15.66). The CuSO4 footbaths were useful to prevent HHE and indicate that automatic stationary flushing with only water had no beneficial effect on ID or HHE. The claw horn of cows walking through CuSO4 became harder and the claw horn of cows that had their hind feet flushed with water became softer compared with the controls.

The aim of this study was genetic analyses of claw health in Norwegian Red. Claw health status at claw trimming has, since 2004, been recorded in the Norwegian Dairy Herd Recording System. The claw trimmer records whether the cow has normal (healthy) claws or if one or more claw disorders are present. Nine defined claw disorders were recorded: corkscrew claw (CSC), heel horn erosion (HH), dermatitis (DE), sole ulcer (SU), white line disorder (WLD), hemorrhage of sole and white line (HSW), interdigital phlegmon (IDP), lameness (LAME), and acute trauma (AT). Data from 2004 to 2011, with a total of 204,892 claw health records, were analyzed. The disorders were defined as binary traits with 1 record per cow per lactation. Further, 3 groups of claw disorders were analyzed: infectious claw disorders (INFEC, containing HH, DE, and IDP); laminitis-related claw disorders (LAMIN, containing SU, WLD, and HSW); and overall claw disorder. The 9 single traits and the 3 groups were analyzed using univariate threshold sire models. Multivariate threshold models were performed for the 5 most frequent single traits (CSC, HH, DE, SU, and WLD) and for CSC together with the grouped traits INFEC and LAMIN. Posterior mean of heritability of liability ranged from 0.04 to 0.23, where CSC had the highest heritability. The posterior standard deviations of heritability were low, between 0.01 and 0.03, except for IDP (0.06). Heritability of liability to INFEC and LAMIN were both 0.11 and for overall claw disorders, the heritability was 0.13. Posterior means of the genetic correlation among the 5 claw disorders varied between 0.02 and 0.79, and the genetic correlations between DE and HH (0.65) and between WLD and SU (0.79) were highest. Genetic correlation between INFEC and CSC was close to zero (0.06), between LAMIN and CSC it was 0.31, and between LAMIN and INFEC it was 0.24. The results show that claw disorders are sufficiently heritable for genetic evaluation and inclusion in the breeding scheme. At present, data are scarce with few recorded daughters per sire. Claw trimming records from more herds would therefore be beneficial for routine genetic evaluation of claw health.

International audience; The objective of this study was to evaluate genome-enabled predictions of daughter yield deviations for clinical mastitis in Norwegian Red cows within and between environments according to mastitis pathogen status. Genome-based predictions of daughter yield deviations for clinical mastitis for 1,126 bulls within and between 5 environments were performed using Bayesian ridge regression. The environments were defined as herd-5-yr classes with the following prevalence of bacteriological milk samples found positive for contagious mastitis pathogens: 25% and

SummaryAdventitious shoots were regenerated, using leaf segments from in vitro cultures of a Norwegian red raspberry (Rubus idaeus L.) selection, N71-B3 (‘Distad’ × ‘Viking’). 1H-indole-3-butanoic acid (IBA) in combination with either N-(phenylmethyl-1H-purin-6-amine (BA) or N-phenyl-N’-l,2,3-thiadiazol-5-ylurea(thidiazuron or TDZ), were added to MS media. Media were evaluated for regeneration rates, rooting ability of regenerants, genetic stability of phenotype and DNA fragment profiles. There was a positive association between low growth regulator concentration as compared with high regeneration and high rooting percentages as well as low off-type production. B A gave a higher regeneration and rooting percentage than TDZ, while the development of off-types was at a comparable level at similar cytokinin concentrations (BAhigh vs. TDZlow). When DNA fingerprinting was used to compare divergent phenotypes with control plants, no variation in fragment patterns could be detected by hybridization of Hae III di...

To measure the background concentration of phylloerythrin in plasma from clinically healthy sheep, goats, cattle and horses on pasture.Blood samples were taken from 34 sheep of the Dala breed, 20 female Norwegian dairy goats, 35 Norwegian Red cows and 34 horses of different breeds. All animals were grazing green pasture when blood samples were taken. Blood samples were collected from each of four clinically healthy newborn lambs, goats, calves and foals, and pooled into one sample per species. Plasma samples were analysed for phylloerythrin by fluorescence spectroscopy, using a Perkin-Elmer LS-50B luminescence spectrometer equipped with a red-sensitive photomultiplier. The fluorescence spectra of phylloerythrin in plasma from the adult ruminants were compared with those in plasma from the neonatal ruminants, to which a known concentration of phylloerythrin had been added.Plasma obtained from the adult ruminants had spectral characteristics similar to those of phylloerythrin, namely weak emission peaks at 650 and 711 nm, when excited at 425 nm. Emission spectra obtained from plasma from the neonatal ruminants showed no fluorescence at these wavelengths. On average, 0.012 (SD 0.004), 0.06 (SD 0.04), and 0.05 (SD 0.03) micromol/l phylloerythrin were present in plasma samples from the sheep, goats, and cattle, respectively. The fluorescence spectra of plasma from the newborn foals were similar to spectra of plasma from adult horses, with weak emission at 669 nm.Small concentrations of phylloerythrin were detected in plasma from clinically healthy sheep, goats and cattle, but none could be detected in plasma from clinically healthy horses.

Phytoestrogens are hormone-like substances in plants that can substantially influence human health (positively or negatively), and when fed to dairy cows are partly transferred to their milk. The aim of this study was to investigate the effects of varying the botanical composition and regrowth interval of legume-grass silage on phytoestrogen intake and milk phytoestrogen concentrations. In one experiment, 15 Swedish Red dairy cows were fed 2- or 3-cut red clover-grass silage, or 2-cut birdsfoot trefoil-grass silage. In a second experiment, 16 Norwegian Red dairy cows were fed short-term ley silage with red clover or long-term ley silage with white clover, and the effects of supplementation with α-tocopherol were also tested. High concentrations of formononetin and biochanin A were found in all silage mixtures with red clover. The milk concentration of equol was highest for cows on the 2-cut red clover-grass silage diet (1,494 μg/kg of milk). Because of the metabolism of biochanin A, genistein, and prunetin, their concentrations in milk and the apparent recovery were low. Coumestrol was detected in only short-term and long-term ley silage mixtures, and its milk concentration was low. Concentrations of secoisolariciresinol and matairesinol were higher in 2-cut birdsfoot trefoil-grass and long-term ley silage mixtures, those with legume species other than red clover, and the highest grass proportions. The 2-cut birdsfoot trefoil-grass silage diet also resulted in higher enterolactone concentration than the other diets (226 μg/kg of milk). Lengthening the regrowth interval increased the intake of secoisolariciresinol and decreased the recovery of lignans. Feeding long-term ley silage resulted in higher milk lignan concentrations but lower milk isoflavone concentrations than feeding short-term ley silage. The apparent recovery of all phytoestrogens except prunetin was highest on the 2-cut birdsfoot trefoil-grass silage diet. No effect of α-tocopherol supplementation was observed on milk concentrations of any of the measured phytoestrogens. Variations were observed in milk concentrations of phytoestrogens, especially of equol, among cows, which could not be explained by variations in diet composition or phytoestrogen intake. The results show that milk phytoestrogen concentration is strongly influenced by silage botanical composition, but questions regarding phytoestrogen metabolism remain to be answered.

Conventionally, levels of inbreeding for livestock animals are estimated analyzing pedigree data. The recent availability of high density SNP arrays for the bovine genome has provided the opportunity for investigation of levels of autozygosity based on runs of homozygosity (ROH). A run of homozygosity is a continuous segment of DNA sequence without heterozygosity in the diploid state. Levels of homozygosity derived from ROH are recognized as potential inbreeding measure in livestock animals. Here we compare levels of homozygosity derived from proportion of ROH in genome (FROH) and pedigree inbreeding estimates (Fped). We analyzed genotype and pedigree data from 1687 animals from four cattle breeds (Brown Swiss, Norwegian Red, Tyrol Grey and Fleckvieh – dual purpose Simmental). Distribution of ROH was obtained by PLINK software, while proportions of genome in ROH were calculated for five cut-off lengths (1Mb, 2Mb, 4Mb, 8Mb, 16Mb). Pedigree data was analyzed by PEDIG software and inbreeding coefficients for complete pedigree (average complete generation equivalents ranging from 6.46 to 9.02 for the four breeds) and over 5 generations were calculated. Levels of autozygosity from pedigree information were similar to those derived from ROH >8Mb. The strongest correlations with pedigree inbreeding coefficients were obtained between levels of homozygosity derived from ROH for cut-off length of 4Mb (FROH4 ) and inbreeding coefficients for complete pedigrees (FpedT) varying from 0.619 for Norwegian Red up to 0.705 for Tyrol Grey. We conclude that proportion of the genome arranged in long homozygous segments provides a good indication of inbreeding levels and that the choice of cut-off lengths of ROH allows determining autozygosity derived from recent or remote ancestors.

Twenty-four low dry matter (DM) silages differing in fermentation quality were harvested at the same time from a crop that consisted mainly of timothy (Phleum pratense), and meadow fescue (Festuca pratensis). The silage samples were analysed by gas chromatography (GC) – mass spectrometry and gas chromatography – flame ionisation detection in order to determine and quantify volatiles present in silage. The voluntary intake of the 24 silages had been measured in a previous feeding trial with growing steers of Norwegian Red. Thirteen esters, five aldehydes, three alcohols, and one sulphide were identified and quantified. A total of 51 variables describing the chemical composition of the silages were included in a partial least-squares regression, and the relationship of silage fermentation quality to voluntary intake was elucidated. The importance of variables describing silage fermentation quality in relation to intake was judged from a best combination procedure, jack-knifing, and empirical correlations of the variables to intake. The GC-analysed compounds were mainly present in poorly fermented silages. However, compared with other explanatory chemical variables none of these compounds was of importance for the voluntary intake as evaluated by partial least-squares regression. A validated variance of 71% in silage DM intake was explained with the selected variables: total acids (TA), total volatile fatty acids (TVFA), lactic acid/total acid ratio and propionic acid. In this study extent (by the variable TA) and type of silage fermentation (by TVFA) influenced intake. Further, it is suggested that by restricting the fermentation in low DM grass silages the potential intake of silage DM is maximised.

Background Red deer (Cervus elaphus) have been an important human resource for millennia, experiencing intensive human influence through habitat alterations, hunting and translocation of animals. In this study we investigate a time series of ancient and contemporary DNA from Norwegian red deer spanning about 7,000 years. Our main aim was to investigate how increasing agricultural land use, hunting pressure and possibly human mediated translocation of animals have affected the genetic diversity on a long-term scale. Results We obtained mtDNA (D-loop) sequences from 73 ancient specimens. These show higher genetic diversity in ancient compared to extant samples, with the highest diversity preceding the onset of agricultural intensification in the Early Iron Age. Using standard diversity indices, Bayesian skyline plot and approximate Bayesian computation, we detected a population reduction which was more prolonged than, but not as severe as, historic documents indicate. There are signs of substantial changes in haplotype frequencies primarily due to loss of haplotypes through genetic drift. There is no indication of human mediated translocations into the Norwegian population. All the Norwegian sequences show a western European origin, from which the Norwegian lineage diverged approximately 15,000 years ago. Conclusions Our results provide direct insight into the effects of increasing habitat fragmentation and human hunting pressure on genetic diversity and structure of red deer populations. They also shed light on the northward post-glacial colonisation process of red deer in Europe and suggest increased precision in inferring past demographic events when including both ancient and contemporary DNA. © 2012 Rosvold et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The main objective was to evaluate the potential of grass silages of very high quality to support a high milk yield with a low or moderate, or even without concentrate supplementation. Production responses to increased levels of concentrate supplementation with 3 primary growth grass silages differing in digestibility were studied using 66 Norwegian Red dairy cows. Roundbale silage was produced from a timothy-dominated sward at very early (H1), early (H2), and normal (H3) stages of crop maturity. Crops were rapidly wilted (

In order to conform with current EC standards with regard to antibiotic cover, the Norwegian Cattle Association is currently investigating the use of Biladyl® as an alternative to the milk-based extender which has been traditionally used in Norway. A study was carried out to investigate the effect of using semen frozen with either milk extender or Biladyl® on the outcome of in vitro fertilization and embryo culture. Semen from 6 Norwegian Red bulls was used. There was a significant difference p

Klauvlidelser forekommer hyppigere i løsdriftsfjøs enn i båsfjøs. På grunn av stadig økt andel storfe som er oppstallet i løsdrift blir det mer og mer aktuelt å forebygge klauvlidelser. I 2004 ble helsekort klauv innført i Norge. Helsekort klauv er en integrert del av kukontrollen, der veterinærer, klauvskjærer eller bonden selv registrerer hendelser ved klauvskjæring. Helsekort klauv har ti koder, normal, halthet, akutt skade, korketrekkerklauv, v-formet hornforråtnelse, hudbetennelse, klauvspalteflegmone, såleknusning, løsning/byll i den hvite linje og blødning i såle/den hvite linje. Denne masteroppgaven er basert på data som er samlet inn gjennom registreringer i helsekort klauv. Dette er den første genetiske analysen av norske klauvhelsedata. Målet med denne masteroppgaven var å beregne arvegrader og genetiske korrelasjoner for klauvlidelsene, samt estimere avlsverdier for oksene og beregne genetisk trend, korrelasjoner mellom avlsverdier for klauvlidelser og avlsverdier for andre egenskaper, og vurdere potensialet for videre bruk av dataene. Totalt 201 186 klauvdata var rapportert inn i perioden fra januar 2004 til november 2010. I løpet av årene har det vært en stadig økning av antall rapporteringer. Omlag 79 % av observasjonene var friske klauver. Den mest utbredte klauvlidelsen er korketrekkerklauv (8,9 %). Dataene ble analysert med en lineær farmodell. Arvegradene som ble funnet var: 0,055 for alle klauvlidelsene samlet, 0,002 for halthet, 0,071 for korketrekkerklauv, 0,01 for v-formet hornforråtnelse, 0,015 for hudbetennelse, 0,019 for såleknusning, 0,008 for løsning/byll i den hvite linje og 0,005 for blødning i såle/den hvite linje. For akutt skade og klauvspalteflegmone var arvegraden null. Det ble beregnet genetiske korrelasjoner mellom korketrekkerklauv, v-formet hornforråtnelse, såleknusning og løsning/byll i den hvite linje, som er de fire mest vanlige klauvlidelsene. De genetiske korrelasjonene var: 0,759 ± 0,09 mellom såleknusning og løsning/byll i den hvite linje, 0,497 ± 0,084 mellom såleknusning og korketrekkerklauv og 0,340 ± 0,131 mellom såleknusning og v-formet hornforråtnelse. De øvrige genetiske korrelasjonene var nær null. Genetisk trend for v-formet hornforråtnelse var relativt flat for perioden 1998 til 2005. De øvrige klauvlidelsene varierte mellom år, men viste ingen klar utvikling for perioden. Det var liten sammenheng mellom avlsverdiene for klauvlidelser og den offisielle beinindeksen (korrelasjon < 0,21). Forutsatt flere registreringer, ser de norske klauvdataene ut til å være egnet som grunnlag for til å lage indekser for de enkelte klauvlidelsene. Claw disorders occur more frequently in loose housing systems than in tie-stall barns. Due to the increasing number of cattle being housed in loose housing barns, the need to prevent claw disorders increases. In 2004 the claw health recording card was introduced in Norway. The claw health card is an integrated part of the Norwegian Dairy Herd Recording System. Veterinarians, claw trimmers or the farmers enter information at claw trimming. The claw health recording has ten codes: normal, lameness, acute injury, corkscrew claw, v-shaped heel horn erosion, dermatitis, interdigital phlegmon, sole ulcer, white line disease, and sole and white line haemorrhage. This thesis is based on data from the claw health recordings, and is the first genetic analysis of the Norwegian claw health data. The objectives of this thesis were to estimate the heritabilities of and genetic correlations among claw disorders, and to estimate breeding values of the sires and calculate genetic trends for claw disorders in Norwegian Red, to calculate correlations between breeding values for claw disorders and breeding values for other traits, and evaluate the potential for further use of the data. There were a total of 201,186 observations in the data set, which were reported in the period January 2004 to November 2010. Over the years there has been a steady increase in the number of records. About 79 % of the observations were healthy claws. The most common claw disorder was corkscrew claw (8.9 %). The data were analyzed with linear sire models. The heritabilities were 0.055 for all claw disorders combined, 0.002 for lameness, 0.071 for corkscrew claw, and 0.010 for v-shaped heel horn erosion, 0.015 for dermatitis, 0.019 for sole ulcer, 0.008 for white line disease and 0.005 for sole and white line haemorrhage. Heritabilities for acute injury and interdigital phlegmon were found to be zero. Genetic correlations were estimated among corkscrew claw, v-shaped heel horn erosion, sole ulcer and white line disease, the four most frequent claw disorders in Norway. The genetic correlations were 0.759 ± 0.09 between sole ulcer and white line disease, 0.497 ± 0.084 between sole ulcer and corkscrew claw, 0.340 ± 0.131 between sole ulcer and v-shaped heel horn erosion, others were close to zero. The genetic trend for v-shaped heel horn erosion was flat for the period 1998 to 2005. The other claw disorders showed more variation between years, but there were no obvious trend. The correlations between the breeding values for claw disorders and the official leg index were rather weak (< 0.21). Assuming increasing number of registrations, the Norwegian claw data should be suitable as a basis for genetic evaluation for the claw disorders in the future.

Normande (NO) × Holstein (HO) crossbred cows (n = 251), Montbeliarde (MO) × HO crossbred cows (n = 503), and Scandinavian Red (SR) × HO crossbred cows (n = 321) were compared with pure HO cows (n = 416) for fertility, somatic cell score (SCS), and 305-d projected milk, fat, and protein production during their first 5 lactations. The SR was a combination of Swedish Red and Norwegian Red. Cows were housed in 6 commercial herds in California and calved from June 2002 to January 2009. The NO, MO, and SR sires of crossbred cows were artificial insemination bulls via imported semen. The NO × HO, MO × HO, and SR × HO cows had fewer days to first breeding, enhanced first-service conception rates, higher pregnancy rates, and 12 to 26 fewer days open than did pure HO cows during their first 5 lactations. Mean SCS across lactations was similar for NO × HO (3.25) and pure HO (3.27) cows; however, MO × HO (2.98) and SR × HO (3.12) cows were significantly lower for SCS than were pure HO cows. The MO × HO cows and SR × HO cows were only 3 and 4% lower, respectively, than pure HO cows for 305-d projected production of fat (kg) plus protein (kg); however, NO × HO cows were 10% lower than pure HO cows for fat plus protein production. Therefore, the MO and SR are candidate breeds for crossbreeding with HO to improve the fertility and udder health of herds with high mean production.

Background This study was carried out to determine if an association exists between the shape of the lactation curve before it is influenced by the event of conception and the time from calving to conception in Norwegian dairy cattle. Lactation curves of Norwegian Red cows during 5 to 42 days in milk (DIM) were compared between cows conceiving between 43 and 93 DIM and cows conceiving after 93 DIM. Methods Data from 23,049 cows, represented by one lactation each, with 219,538 monthly test days were extracted from the Norwegian Dairy Herd Recording System, which represents 97% of all Norwegian dairy cows. Besides veterinary treatments, these records also included information on daily milk yield at monthly test days. The data were stratified by parity groups (1, 2, and 3 and higher) and time to conception periods (43-93 DIM and >93 DIM). The sample was selected using the following selection criteria: conception later than 42 DIM, calving season July to September, no records of veterinary treatment and the level of energy fed as concentrates between 8.69 and 12.83 MJ. The shape of the lactation curves were parameterized using a modified Wilmink-model in a mixed model analysis. Differences in the parameters of the lactation curves with different conception times were evaluated using confidence intervals. Results Lactation curves characterized by a low intercept and a steep ascending slope and a steep descending slope were associated with early conception across all parities. The peak milk yield was not associated with time of conception. Conclusions A practical application of the study results is the use of the shape of the lactation curve in future herd management. Groups of cows with impaired reproductive performance may be identified due to an unfavorable shape of the lactation curve. Monitoring lactation curves and adjusting the feeding strategy to adjust yield therefore may be useful for the improvement of reproductive performance at herd level.

Background Previous fine mapping studies in Norwegian Red cattle (NRC) in the region 86-90.4 Mb on Bos taurus chromosome 6 (BTA6) has revealed a quantitative trait locus (QTL) for protein yield (PY) around 88 Mb and a QTL for clinical mastitis (CM) around 90 Mb. The close proximity of these QTLs may partly explain the unfavorable genetic correlation between these two traits in NRC. A long range haplotype covering this region was introduced into the NRC population through the importation of a Holstein-Friesian bull (1606 Frasse) from Sweden in the 1970s. It has been suggested that this haplotype has a favorable effect on milk protein content but an unfavorable effect on mastitis susceptibility. Selective breeding for milk production traits is likely to have increased the frequency of this haplotype in the NRC population. Results Association mapping for PY and CM in NRC was performed using genotypes from 556 SNPs throughout the region 86-97 Mb on BTA6 and daughter-yield-deviations (DYDs) from 2601 bulls made available from the Norwegian dairy herd recording system. Highest test scores for PY were found for single-nucleotide polymorphisms (SNPs) within and surrounding the genes CSN2 and CSN1S2, coding for the β-casein and αS2-casein proteins. High coverage re-sequencing by high throughput sequencing technology enabled molecular characterization of a long range haplotype from 1606 Frasse encompassing these two genes. Haplotype analysis of a large number of descendants from this bull indicated that the haplotype was not markedly disrupted by recombination in this region. The haplotype was associated with both increased milk protein content and increased susceptibility to mastitis, which might explain parts of the observed genetic correlation between PY and CM in NRC. Plausible causal polymorphisms affecting PY were detected in the promoter region and in the 5'-flanking UTR of CSN1S2. These polymorphisms could affect transcription or translation of CSN1S2 and thereby affect the amount of αS2-casein in milk. Highest test scores for CM were found in the region 89-91 Mb on BTA6, very close to a cluster of genes coding for CXC chemokines. Expression levels of some of these CXC chemokines have previously been shown to increase in bovine mammary gland cell lines after exposure to bacterial cell wall components. Conclusion Molecular characterization of the long range haplotype from the Holstein-Friesian bull 1606 Frasse, imported into NRC in the 1970s, revealed polymorphisms that could affect transcription or translation of the casein gene CSN1S2. Sires with this haplotype had daughters with significantly elevated milk protein content and selection for milk production traits is likely to have increased the frequency of this haplotype in the NRC population. The haplotype was also associated with increased mastitis susceptibility, which might explain parts of the genetic correlation between PY and CM in NRC.

Grazing Norwegian Red dairy cows were offered one of two roughage supplements ‘baled silage (S) or ryegrass (R)’ on an ad libitum basis for a limited period daily. A third group received concentrate (C). Milk and FCM yields were significantly (P

Three experiments were carried out to study the long-term effects of four combinations of HIGH and LOW concentrate supplementation during the first and second lactation of 72 cows of the Norwegian Red breed: HIGH-HIGH (HH); HIGH-LOW (HL); LOW-HIGH (LH) and LOW-LOW (LL). Basal diets were 0–2 kg hay per cow daily and grass silage ad libitum. Compared with HIGH level supplementation, the LOW level cows consumed 0.4 kg DM more forage in the first lactation and 0.4–1.0 kg DM in the second lactation. Milk yield was lower, respectively 2.5 kg and 1.9 kg 4% FCM (fat-corrected milk) daily, between HIGH and LOW in the first and second lactation, with significant cumulative and residual effects from the first to the second lactation. The difference in daily yield was 3.1 kg 4% FCM between HH and LL vs. 1.8 kg between HH and HL. During weeks 1–4 and 5–14 of the second lactation the average daily production was 2.0 and 1.6 kg higher for HH and HL as compared to LH and LL. Milk protein percentage in the second...

As an extension of a former study, the objectives of this study were to evaluate purebred Holstein (HO; n=140) and crossbred Norwegian Red × Holstein (NRFX; n=142) calves for antibody (AMIR) and cell-mediated immune responses (CMIR) as well as survival. Blood was collected on d 0, 14, and 21, and calves were immunized on d 0 and 14 with type 1 (Candida albicans) and type 2 (hen egg white lysozyme) antigens, which have been shown to induce CMIR and AMIR, respectively. Day 21 background skin-fold measurements of either side of the tail-fold were taken and intradermal injections of test (type 1 antigen) and control (phosphate saline buffer) were administered. Day 23 final skin-fold measurements were taken to assess delayed type hypersensitivity as an indicator of CMIR. Survival data were obtained from CanWest Dairy Herd Improvement. Statistical Analysis System general linear models were used to analyze all immune response and survival data and to determine statistical significance between breeds. Results showed that NRFX had greater primary IgM, IgG, IgG1, and secondary IgG1 antibody response, as well as greater primary IgG1:IgG2 ratio to the type 2 antigen compared with HO. The NRFX also had greater primary IgG1 and IgG2, and secondary IgG2 antibody response as well as greater primary IgG1:IgG2 ratio to the type 1 antigen. The NRFX calves had a tendency toward greater survival from age at immune response testing to calving. No difference was observed between breeds for other secondary antibody response traits or delayed type hypersensitivity. Results indicate NRFX have greater AMIR and therefore may have enhanced defense against extracellular pathogens. This may contribute to increased survival compared with HO. Both breeds, however, likely have similar defense against intracellular pathogens, because no differences in CMIR were observed. In general, these results may suggest that crossbreeding could improve resistance to certain diseases in dairy calves, resulting in decreased input costs to producers for crossbred calves compared with purebred calves. However, more research with larger sample sizes and different breeds should be conducted to confirm these results and obtain a complete picture of the benefits of crossbreeding on immune response traits in calves.

An investigation was made of the potential for attaining high daily live weight (LW) gain, high feeding efficiency and carcass quality in Norwegian Red (NRF) bulls fed grass silage harvested at early stages of maturity, supplemented with minimal amounts of concentrates. Roundbale silage was produced from timothy-dominated swards harvested at three stages of maturity: stages 1 and 2 were dominated by tillers in stem elongation with two and three visible nodes, respectively, and stage 3 by tillers at early heading, with visible heads, but without head stems. Crops were wilted rapidly and a formic acid-based additive applied. All silages were preserved with restricted fermentation. Silage DOMD values were 0·747, 0·708 and 0·647 for harvesting time (H) 1, 2 and 3 respectively. Silages were fed ad libitum as sole feed, or supplemented with 2–4 kg concentrate at increasing LW, to six bulls per treatment from age 7 months (288 kg) to slaughter at 575 kg. Daily LW gain for bulls fed unsupplemented silage was 1423, 1262 and 936 g, respectively, for H1, H2 and H3, and 1570, 1567 and 1357 g for supplemented bulls. For concentrate-supplemented bulls, higher energy intake increased proportions of carcass fat more for H1 (0·163) than for H2 (0·134) but the same amount of energy was retained in the carcass per MJ net energy growth intake for these two groups. It is concluded that, apart from milk and concentrate fed to calves during the first months, an intensive beef production with NRF bulls finished before 15 months of age may be based entirely on local grass resources.

Background The objectives of this study were to investigate whether there were differences between Norwegian Red cows in conventional and organic farming with respect to reproductive performance, udder health, and antibiotic resistance in udder pathogens. Methods Twenty-five conventional and 24 organic herds from south-east and middle Norway participated in the study. Herds were matched such that geographical location, herd size, and barn types were similar across the cohorts. All organic herds were certified as organic between 1997 and 2003. All herds were members of the Norwegian Dairy Herd Recording System. The herds were visited once during the study. The relationship between the outcomes and explanatory variables were assessed using mixed linear models. Results There were less > 2nd parity cows in conventional farming. The conventional cows had higher milk yields and received more concentrates than organic cows. Although after adjustment for milk yield and parity, somatic cell count was lower in organic cows than conventional cows. There was a higher proportion of quarters that were dried off at the herd visit in organic herds. No differences in the interval to first AI, interval to last AI or calving interval was revealed between organic and conventional cows. There was no difference between conventional and organic cows in quarter samples positive for mastitis bacteria from the herd visit. Milk yield and parity were associated with the likelihood of at least one quarter positive for mastitis bacteria. There was few S. aureus isolates resistance to penicillin in both management systems. Penicillin resistance against Coagulase negative staphylococci isolated from subclinically infected quarters was 48.5% in conventional herds and 46.5% in organic herds. Conclusion There were no large differences between reproductive performance and udder health between conventional and organic farming for Norwegian Red cows.

The data set used in the present study was obtained from 20 energy metabolism studies involving 579 lactating dairy cows (511 Holstein-Friesian, 36 Norwegian Red, and 32 Jersey-Holstein crossbreds) varying in genetic merit, lactation number, stage of lactation, and live weight. These cows were offered diets based on grass silage (n=550) or fresh grass (n=29), and their energy intake and outputs, including methane energy (CH 4 -E), were measured in indirect open-circuit respiration calorimeter chambers. The objective was to use these data to evaluate relationships between CH 4 -E output and a range of factors in animal production and energetic efficiency in lactating dairy cows under normal feeding regimens. The CH 4 -E as a proportion of milk energy output (E l ), E l adjusted to zero energy balance (E l(0) ), or intakes of gross energy (GE), digestible energy (DE), or metabolizable energy (ME) was significantly related to a wide range of variables associated with milk production (E l and E l(0) ) and energy parameters (energy intake, metabolizability, partitioning, and utilization efficiencies). Three sets of linear relationships were developed with experimental effects removed. The CH 4 -E/GE intake (r 2 =0.50–0.62) and CH 4 -E/E l (r 2 =0.41–0.68) were reduced with increasing feeding level, E l /metabolic body weight (MBW; kg 0.75 ), E l(0) /MBW, GE intake/MBW, DE intake/MBW, and ME intake/MBW. Increasing dietary ME/DE decreased CH 4 -E/E l (r 2 =0.46) and CH 4 -E/GE intake (r 2 =0.72). Dietary ME concentration and ME/GE were also negatively related to CH 4 -E/GE intake (r 2 =0.47). However, increasing heat production/ME intake increased CH 4 -E as a proportion of E l (r 2 =0.41), E l(0) (r 2 =0.67) and energy intake (GE, DE, and ME; r 2 =0.62 and 0.70). These proportional CH 4 -E variables were reduced with increasing ratios of E l /ME intake and E l(0) /ME intake and efficiency of ME use for lactation (r 2 =0.49–0.70). Fitting CH 4 -E/E l or CH 4 -E/E l(0) against these energetic efficiencies in quadratic rather than linear relationships significantly increased r 2 values (0.49–0.67 vs. 0.59–0.87). In conclusion, CH 4 -E as a proportion of energy intake (GE, DE, and ME) and milk production (E l and E l(0) ) can be reduced by increasing milk yield and energetic efficiency of milk production or by reducing energy expenditure for maintenance. The selection of dairy cows with high energy utilization efficiencies and milk productivity offers an effective approach to reducing enteric CH 4 emission rates.

Physical restrictions at the feed barrier may cause skin lesions, especially on the necks of cows. Thus, some aspects of the functionality of feed barrier design can be assessed by observing skin lesions on the necks of dairy cattle. In this study, 232 herds, mainly of the breed Norwegian Red (94%), were investigated between September 2006 and June 2007. The herds were categorized into 2 groups. One data set, based on 1,148 cows (115 herds), was from farms using feed barriers with post-and-rail designs (86 herds) and combinations of barriers (29 herds). The second data set, which included 1,168 cows (117 herds), was from farms using only vertical feed barriers such as tombstone, diagonal, and headlock. Skin lesions were observed on the necks of 21% of the 2,335 cows observed. Forty-two percent of cows had neck lesions in herds with post-and-rail barriers, whereas 4% of cows had neck lesions in herds with vertical barriers and 30% of cows had neck lesions in herds with a combination of both horizontal and vertical barriers. Alternating logistic regression was used to identify factors associated with skin lesions on the necks of cows. The estimates were transformed into odds ratios (OR). In herds in which post-and-rail design barriers were used, the following risk factors were associated with skin lesions: manger front lower than 63 cm versus higher (OR=3.6), no feed stalls versus feed stalls (OR=10.9), mixed rations versus no mixed ration (OR=3.8), cows later than 30 DIM versus earlier (OR=1.9), cows with low milk production versus those producing 1,000 kg higher (OR=1.4), feeding frequency of less than 3 times daily versus 3 and more times daily (OR=1.9), using only post-and-rail feed barriers (OR=1.8) versus using combinations, and farmer disagreement with the statement "animals experience physical pain as humans do" (OR=1.9) versus agreement. Diagonal barriers, categorized as vertical barriers, increased the association of skin lesions with increasing manger front heights. Feed barrier design was associated with skin lesions on the necks of cows, particularly for barriers of a post-and-rail design. To minimize the risk of neck lesions, the top rail height of post-and-rail barriers should be above 109 cm, especially for cows taller than 126 cm, and the manger front should not be lower than 63 cm. Feed stalls, feeding frequency of 3 or more times daily, and the feeding of unmixed rations were also identified as preventive factors.

Progesterone profiles in Norwegian Red cows were categorized, and associations between the occurrence of irregularities in the profiles and the commencement of luteal activity were investigated. The cows were managed in 3 feeding trials from 1994 to 2001 and from 2005 to 2008 at the Norwegian University of Life Sciences. The cows were followed from calving, and the milk samples collected represented 502 lactations from 302 cows. Milk samples for progesterone analysis were taken 3 times weekly from 1994 throughout 1998 and from 2005 to 2008 and 2 times weekly from 1999 to 2001. Commencement of luteal activity was defined as the first day of 2 consecutive measurements of progesterone concentrationor=3 ng/mL not earlier than 10 d after calving. Delayed ovulation type I was defined as consistently low progesterone concentration,3 ng/mL foror=50 d postpartum. Delayed ovulation type II was defined as prolonged interluteal interval with milk progesterone measurements3 ng/mL foror=12 d between 2 luteal phases. Persistent corpus luteum (PCL) type I was defined as delayed luteolysis with milk progesteroneor=3 ng/mL foror=19 d during the first estrous cycle postpartum. Persistent corpus luteum type II was defined as delayed luteolysis with milk progesteroneor=3 ng/mL foror=19 d during subsequent estrous cycles before first artificial insemination. Delayed ovulation type I was present in 14.7%, delayed ovulation type II in 2.8%, PCL type I in 6.7%, and PCL type II in 3.3% of the profiles. Commencement of luteal activity was related to milk yield, parity, PCL type I, and the summated occurrence of PCL type I and II. The least squares means for the interval to commencement of luteal activity were 24.2 d when PCL type I and II were present and 29.5 d when PCL type I and II were absent. The likelihood of pregnancy to first service was not affected in cows with a history of PCL when artificial insemination was carried out at progesterone concentrations3 ng/mL (i.e., during estrus); however, cows that had experienced PCL were more likely to be inseminated during a luteal phase. The occurrence of delayed ovulation and PCL in Norwegian Red cows was less than that reported in most other dairy populations.

Relationships among commencement of luteal activity (C-LA), milk yield, and energy balance (EB) were investigated in 3 selection lines of Norwegian Red cows at the Norwegian University of Life Sciences from 1994 through 2001. The cows were selected for low genetic merit for milk yield (LMP), high genetic merit for milk yield (HMP), and a combination of high indices for milk yield and fertility (HI). Breeding values for fertility were based on 56-d nonreturn rate. The material included 268 lactations from 147 cows. Milk samples for progesterone analysis were drawn 3 times weekly from 1994 through 1998, and 2 times weekly from 1999 to 2001. Commencement of luteal activity was defined as the first 2 consecutive measurements of progesterone concentration >3 ng/mL not earlier than 10 d after calving. Selection line was significantly related to C-LA, so that the least squares mean days from calving to C-LA were 22.5, 30.4, and 27.2 d for LMP, HMP, and HI cows, respectively. The HMP cows produced more milk than the LMP cows. The average milk yield in the sixth week of lactation was 24.0, 27.1, and 25.3 kg for LMP, HMP, and HI cows, respectively. The interval to C-LA decreased for the HMP and HI cows after phenotypic adjustment for EB in the model. Least squares means for the interval to C-LA were 23.2, 29.7, and 25.6 d for the LMP, HMP, and HI cows, respectively, in a model that included parity, selection lines, and EB as covariates. Cumulated EB during the first 4 wk of lactation, which itself differed between selection lines, did not fully account for differences in interval to C-LA between selection lines. Thus, the results of the present investigation indicate that selection for milk yield negatively affects C-LA over and above the effects caused by concurrent changes in EB. The increase in days to C-LA caused by selection for high yields can be reduced if selection for milk yield is combined with fertility in the breeding program.

Health and fertility are complex traits, and the phenotype for one trait may affect the phenotype of one or more other traits. For instance, disease in early lactation may impair a cow's ability to show estrus and to conceive after insemination. The objectives of the present study were to explore phenotypic and genetic relationships among health and fertility traits in Norwegian Red cows using a recursive effects model, which allows disentangling causal effects of phenotypes from the genetic and environmental correlations among traits. Records of interval from calving to first insemination (CFI), nonreturn rate within 56 d after first insemination (NR56), clinical mastitis (CM), ketosis (KET), and retained placenta from 55,568 first-lactation daughters of 1,577 Norwegian Red sires were analyzed. Trivariate recursive Gaussian-threshold models were used to analyze the 2 fertility traits (CFI and NR56) together with 1 disease trait in each analysis. The estimated structural coefficients of the recursive models imply that presence of KET or retained placenta lengthened CFI, whereas causal effects from CM to fertility were negligible. Recursive effects of disease on NR56, and of CFI on NR56, were all close to zero. Genetic correlations between health and fertility traits were low or moderate. The strongest genetic correlation was between KET and CFI (0.29), whereas genetic correlations between CM and NR56 and between CFI and NR56 were nil. In general, selection against disease is expected to slightly improve fertility (shorter CFI and higher NR56) as a correlated response and vice versa. The present results suggest that the use of structural-equation models, such as the one used here, may enhance our understanding of complex relationships among traits.

A high resolution SNP map was constructed for the bovine casein region to identify haplotype structures and study associations with milk traits in Norwegian Red cattle. Our analyses suggest separation of the casein cluster into two haplotype blocks, one consisting of the CSN1S1, CSN2 and CSN1S2 genes and another one consisting of the CSN3 gene. Highly significant associations with both protein and milk yield were found for both single SNPs and haplotypes within the CSN1S1-CSN2-CSN1S2 haplotype block. In contrast, no significant association was found for single SNPs or haplotypes within the CSN3 block. Our results point towards CSN2 and CSN1S2 as the most likely loci harbouring the underlying causative DNA variation. In our study, the most significant results were found for the SNP CSN2_67 with the C allele consistently associated with both higher protein and milk yields. CSN2_67 calls a C to an A substitution at codon 67 in β-casein gene resulting in histidine replacing proline in the amino acid sequence. This polymorphism determines the protein variants A1/B (CSN2_67 A allele) versus A2/A3 (CSN2_67 C allele). Other studies have suggested that a high consumption of A1/B milk may affect human health by increasing the risk of diabetes and heart diseases. Altogether these results argue for an increase in the frequency of the CSN2_67 C allele or haplotypes containing this allele in the Norwegian Red cattle population by selective breeding.

The objective of this study was to compare the immune response of Holstein and Norwegian Red × Holstein calves on 13 commercial Canadian dairy farms. Data were collected on 135 calves, 68 Holstein and 67 Norwegian Red × Holstein calves aged between 2 and 6 mo. The calves were immunized with hen egg white lysozyme to induce antibody-mediated immune response. Candida albicans was used as an in vivo indicator of cell-mediated immune response, with delayed-type hypersensitivity used as the indicator. Antibody response to hen egg white lysozyme (IgG, IgG1, and IgG2) was measured by ELISA. Calves of both breed groups produced a significant primary and secondary antibody-mediated immune response, as well as a delayed-type hypersensitivity reaction. The Norwegian Red×Holstein produced a greater primary IgG antibody-mediated immune response (d 14, and d 14 minus d 0) when compared with the Holstein. No differences were observed between the breeds for secondary response or anti-hen egg white lysozyme isotype (IgG1 or IgG2) production or the ratio of IgG1:IgG2. There was no effect of breed on delayed-type hypersensitivity. Nonetheless, high and low immune responders could be identified in both breed groups, but with no difference in the proportion of high and low responders observed for either antibody-mediated immune response or cell-mediated immune response between breed groups.

A Gaussian-threshold model is described under the general framework of structural equation models for inferring simultaneous and recursive relationships between binary and Gaussian characters, and estimating genetic parameters. Relationships between clinical mastitis (CM) and test-day milk yield (MY) in first-lactation Norwegian Red cows were examined using a recursive Gaussian-threshold model. For comparison, the data were also analyzed using a standard Gaussian-threshold, a multivariate linear model, and a recursive multivariate linear model. The first 180 days of lactation were arbitrarily divided into three periods of equal length, in order to investigate how these relationships evolve in the course of lactation. The recursive model showed negative within-period effects from (liability to) CM to test-day MY in all three lactation periods, and positive between-period effects from test-day MY to (liability to) CM in the following period. Estimates of recursive effects and of genetic parameters were time-dependent. The results suggested unfavorable effects of production on liability to mastitis, and dynamic relationships between mastitis and test-dayMYin the course of lactation. Fitting recursive effects had little influence on the estimation of genetic parameters. However, some differences were found in the estimates of heritability, genetic, and residual correlations, using different types of models (Gaussian-threshold vs. multivariate linear).

First-lactation records on 836,452 daughters of 3,064 Norwegian Red sires were used to examine associations between culling in first lactation and 305-d protein yield, susceptibility to clinical mastitis, lactation mean somatic cell score (SCS), nonreturn rate within 56 d in heifers and primiparous cows, and interval from calving to first insemination. A Bayesian multivariate threshold-linear model was used for analysis. Posterior mean of heritability of liability to culling of primiparous cows was 0.04. The posterior means of the genetic correlations between culling and the other traits were -0.41 to 305-d protein yield, 0.20 to lactation mean SCS, 0.36 to clinical mastitis, 0.15 to interval from calving to first insemination, -0.11 to 56-d nonreturn as heifer, and -0.04 to 56-d nonreturn as primiparous cow. As much as 66% of the genetic variation in culling was explained by genetic variation in protein yield, clinical mastitis, interval of calving to first insemination, and 56-d nonreturn in heifers, whereas contribution from the SCS and 56-d nonreturn as primiparous cow was negligible, after taking the other traits into account. This implies that for breeds selected for a broad breeding goal, including functional traits such as health and fertility, most of the genetic variation in culling will probably be covered by other traits in the breeding goal. However, in populations where data on health and fertility is scarce or not available at all, selection against early culling may be useful in indirect selection for improved health and fertility. Regression of average sire posterior mean on birth-year of the sire indicate a genetic change equivalent to an annual decrease of the probability of culling in first-lactation Norwegian Red cattle by 0.2 percentage units. This genetic improvement is most likely a result of simultaneous selection for improved milk yield, health, and fertility over the last decades First-lactation records on 836,452 daughters of 3,064 Norwegian Red sires were used to examine associations between culling in first lactation and 305-d protein yield, susceptibility to clinical mastitis, lactation mean somatic cell score (SCS), nonreturn rate within 56 d in heifers and primiparous cows, and interval from calving to first insemination. A Bayesian multivariate threshold-linear model was used for analysis. Posterior mean of heritability of liability to culling of primiparous cows was 0.04. The posterior means of the genetic correlations between culling and the other traits were -0.41 to 305-d protein yield, 0.20 to lactation mean SCS, 0.36 to clinical mastitis, 0.15 to interval from calving to first insemination, -0.11 to 56-d nonreturn as heifer, and -0.04 to 56-d nonreturn as primiparous cow. As much as 66% of the genetic variation in culling was explained by genetic variation in protein yield, clinical mastitis, interval of calving to first insemination, and 56-d nonreturn in heifers, whereas contribution from the SCS and 56-d nonreturn as primiparous cow was negligible, after taking the other traits into account. This implies that for breeds selected for a broad breeding goal, including functional traits such as health and fertility, most of the genetic variation in culling will probably be covered by other traits in the breeding goal. However, in populations where data on health and fertility is scarce or not available at all, selection against early culling may be useful in indirect selection for improved health and fertility. Regression of average sire posterior mean on birth-year of the sire indicate a genetic change equivalent to an annual decrease of the probability of culling in first-lactation Norwegian Red cattle by 0.2 percentage units. This genetic improvement is most likely a result of simultaneous selection for improved milk yield, health, and fertility over the last decades

SummaryTeat penetration into transparent silicone rubber teatcup liners was recorded during milking of 90 cows on 15 farms. The cows were of Norwegian Red Cattle breed with mean teat length close to the average for the breed. The mean teat length was 54 and 48 mm for fore and rear teats respectively, and mean penetration at the end of milking was 104 and 94 mm. No significant correlation between initial teat length and teat elongation (difference between penetration and initial length) was found. This indicated that the teatcup liner was more stable on long teats than on short. The distribution of teat penetration at the end of milking showed that a large proportion of fore teats penetrated too far into the liner at the end of milking. The optimal liner length for Norwegian Red Cattle was ~ 150 mm.

A genetic analysis of respiratory disease (RD) in Norwegian Red calves was conducted. This is the first genetic study of calf diseases based on data from the Norwegian health recording system, and RD was chosen because it is the most frequent disease in calves. Records on absence or presence of RD before 180 d of age for 250,212 calves (progeny of 728 Norwegian Red artificial insemination sires) were analyzed with a threshold model. A total of 0.7% of the calves had veterinary treatment of RD before 180 d of age. Heritability of RD in the underlying scale was 0.05, with a 95% credibility interval from 0.02 to 0.09. The "worst" sire had a predicted probability of RD that was more than twice as high as that of the "best" one (1.15 vs. 0.44%). Their 95% credibility intervals for predicted probability of RD, however, overlapped. With improved and more extensive recording of calf diseases, the precision in genetic evaluation of sires could increase considerably. The frequency of RD is very low in the Norwegian Red population at present, so there is not much scope for genetic improvement. However, this study indicates that reasonably precise genetic evaluation of sires for resistance to RD could be feasible.

Genotyping of bovine leucocyte antigen DRB3.2 (BoLA-DRB3.2) in a total of 523 Norwegian Red (NR) cows from two groups selected for high protein yield and low clinical mastitis, respectively, identified 27 previously reported BoLA-DRB3.2 alleles across the groups. Significant differences in BoLA-DRB3.2 allele frequencies were found between the selection groups. Alleles *13, *18, *22 and *27 had a significantly higher frequency in cows selected for low clinical mastitis, while alleles *3, *9, *11 and *26 had a higher frequency in cows selected for high protein yield. Associations between BoLA-DRB3.2 alleles and clinical mastitis were analysed based on mastitis data from 741,072 first-lactation NR cows, of which 452 were genotyped. Alleles *22 and *26 were found to be associated with increased clinical mastitis, while alleles *7, *11, *18 and *24 had a favourable effect on mastitis resistance. Contradictory results from different studies investigating associations between BoLA-DRB3.2 alleles and mastitis indicate that future studies should focus on associations of mastitis with BoLA haplotypes rather than with single BoLA genes.

A Bayesian analysis via Markov chain Monte Carlo methods extending the simultaneous and recursive model of Gianola and Sorensen (2004) was proposed to account for possible population heterogeneity. The method was used to infer relationships between milk yield and somatic cell scores of Norwegian Red cows. Data consisted of test-day records of milk yield and somatic cell score of first-lactation cows during the first 120 d of lactation. Results suggested large negative direct effects from somatic cell score to milk yield and small reciprocal effects from milk yield to somatic cell score. The direct effects were larger in the first 60 d of lactation than in the subsequent period. Bayesian model selection strongly favored the simultaneous and recursive models for milk yield and somatic cell score over the corresponding mixed model without considering simultaneity or recursiveness. Estimated effects between milk yield and somatic cell score seemed to be yield-dependent, larger in higher producing cows than in lower producing cows. Heritability estimates from the simultaneous and recursive models were similar to those from the mixed model, but some genetic correlations differed considerably among models.

Declining reproductive performance is a serious breeding concern in many countries. To reveal the situation in Norwegian cattle, trends in reproductive performance were studied using insemination reports from 1985 to 2005 and data based on herd recording files from 1989 to 2005. The total number of first services was 469.765 in 1985 declining to 335.712 in 2005. The number of recorded herds and animals declined from 21.588 to 14.718 and 360.289 to 309.452 from 1989 to 2005, respectively. Sixty days non-return rate after single inseminations (NR60) increased from 68.1 in 1985 to 72.7% in 2005 (p < 0.001) and the number of services per inseminated animal (NIA) decreased from 1.8 to 1.6 (p < 0.001) from 1985 to 2005. However, return rates 0–3 days post insemination (RR0-3) increased from 6 to 12% in the same period (p < 0.001). NR60 was higher and the RR0-3 was lower in the summer season compared to the winter season during the whole period. A fertility index (FS), has been calculated from the herd recording files each year from 1989 to 2005. The average FS-index did not show a significant trend and the calving interval was also fairly constant between 12.4 and 12.6 months during this period. The average interval from calving to first and last insemination, respectively, increased from a low of 79 and 102 days in 1990 to a high of 86 and 108 days in 2005. Both intervals were consistently longer for cows in first lactation than for cows in later lactations. The percentage of inseminated animals reported culled because of poor fertility decreased from 6.0% in 1989 to 4.6% in 1996 and thereafter again increased to 6% in 2005. In conclusion, most fertility measures, mainly comprising the Norwegian Red (NRF) breed, show a relatively high level of reproductive performance with a positive or a relatively constant trend during the last two decades.

As part of a cross-sectional study of Norwegian Red Cattle, associations of lameness, lesions at the tarsus, claw shapes, claw lesions, and claw trimming with milk yield were examined. Fifty-five tie-stall herds and 57 free-stall herds were sampled by computerized systematic selection, and 2,665 cows were trimmed and limb and claw disorders recorded. After exclusions, 2,599 cows were included in this study. Monthly recordings of milk were extracted from the Norwegian Dairy Herd Recording System. Most claw lesions were mild (score 1). The prevalence of moderate and severe lesions (score 2 and 3) did not exceed 5% for any of the lesions. Associations with test-day milk yield were identified using regression analyses (Proc Mixed) with repeated measurements within lactation and herd as random effect. Lactation curves were fit to visualize differences in changes in milk yield throughout the lactation. Milk yield was generally higher in cows with lesions at the tarsus, heel-horn erosions, and hemorrhages of the white line and the sole than in cows without these lesions, whereas cows with dermatitis yielded less than cows without dermatitis. Cows in lactation 1 with corkscrewed claws had reduced milk yield, whereas cows in lactations 2 and above had increased milk yield compared with cows without corkscrewed claws in respective lactations. Cows in lactation 1 yielded 0.47 +/- 0.15 kg and cows in later lactations yielded 0.51 +/- 0.15 kg more milk on test days after claw trimming than they did before when adjusted for days in milk. In this study, where most disorders were mild, the most evident results were higher milk yield in cows with lesions at the tarsus, heel-horn erosions, and hemorrhages of the white line and the sole than in cows without these lesions. Cows yielded more milk after claw trimming than they did before trimming.

Associations between clinical mastitis (CM) and nonreturn rate within 56 d after first insemination (NR56) were examined in Norwegian Red (NRF) cows. Records on absence or presence of CM within each of the intervals, -30 to 30, 31 to 150, and 151 to 300 d after first calving, and records on NR56 for 620,492 first-lactation daughters of 3,064 NRF sires were analyzed with a Bayesian multivariate threshold liability model. Point estimates of genetic correlations between NR56 and the 3 CM traits were between -0.05 and -0.02. Residual correlations were close to zero, and correlations between herd-5-yr effects on NR56 and CM in the 3 lactation intervals ranged from -0.15 to -0.17. It appears that CM and NR56 in first lactation are independent traits.

Robust threshold models with multivariate Student's t or multivariate Slash link functions were employed to infer genetic parameters of clinical mastitis at different stages of lactation, with each cow defining a cluster of records. The robust fits were compared with that from a multivariate probit model via a pseudo-Bayes factor and an analysis of residuals. Clinical mastitis records on 36 178 first-lactation Norwegian Red cows from 5286 herds, daughters of 245 sires, were analysed. The opportunity for infection interval, going from 30 days pre-calving to 300 days postpartum, was divided into four periods: (i) -30 to 0 days pre-calving; (ii) 1-30 days; (iii) 31-120 days; and (iv) 121-300 days of lactation. Within each period, absence or presence of clinical mastitis was scored as 0 or 1 respectively. Markov chain Monte Carlo methods were used to draw samples from posterior distributions of interest. Pseudo-Bayes factors strongly favoured the multivariate Slash and Student's t models over the probit model. The posterior mean of the degrees of freedom parameter for the Slash model was 2.2, indicating heavy tails of the liability distribution. The posterior mean of the degrees of freedom for the Student's t model was 8.5, also pointing away from a normal liability for clinical mastitis. A residual was the observed phenotype (0 or 1) minus the posterior mean of the probability of mastitis. The Slash and Student's t models tended to have smaller residuals than the probit model in cows that contracted mastitis. Heritability of liability to clinical mastitis was 0.13-0.14 before calving, and ranged from 0.05 to 0.08 after calving in the robust models. Genetic correlations were between 0.50 and 0.73, suggesting that clinical mastitis resistance is not the same trait across periods, corroborating earlier findings with probit models.

Background: Age-related changes in hematologic values are known to occur in many species. Few published studies include repeated measurements of hematologic parameters in calves during the first months of life. Objective: The aim of the present study was to monitor hematologic values by sequential measurements from birth to 6 months of age in 15 healthy calves of the Norwegian Red breed, and compare the results to reference intervals for adult, lactating dairy cows. Methods: Fifteen clinically healthy calves were sampled every week during the first 5 weeks of life and every month thereafter until 6 months of age. Hematologic values were measured using the ADVIA 120 hematology system. Reference intervals were determined for 75 healthy adult cows of the same breed. Results: Compared with adult reference intervals, the MCV was lower and the RBC count was higher in calves throughout the investigation period. Hemoglobin concentration stayed largely within the adult reference interval. Mean MCHC was lower than adult values for 5 weeks, then increased and reached adult values by weeks 10‐ 12. The mean lymphocyte count for calves reached adult reference values at weeks 6‐8, and the mean monocyte count increased steadily until weeks 14‐16. For most leukocytes, interindividual variation was larger during the first 5‐8 weeks of life. The mean platelet count for calves was higher than the adult reference interval until weeks 19‐21 of age. Conclusions: Age-specific reference intervals for calves from birth to 6 month of age are needed for RBC count, MCV, MCHC, red cell distribution width, and platelet and lymphocyte counts. (Vet Clin Pathol. 2006;35:182‐187) � 2006 American Society for Veterinary Clinical Pathology

Pure Holsteins (n = 380) were compared to Normande/Holstein crossbreds (n = 245), Montbeliarde/Holstein crossbreds (n = 494), and Scandinavian Red/Holstein crossbreds (n = 328) for 305-d milk, fat, and protein production during first lactation. Scandinavian Red was a mixture of Swedish Red and Norwegian Red. Cows were housed at 7 commercial dairies in California and calved from June 2002 to January 2005. All Holstein sires and all Holstein maternal grandsires were required to have a code assigned by the National Association of Animal Breeders to assure they were sired by artificial insemination bulls. Daughters of Normande, Montbeliarde, and Scandinavian Red sires were artificial insemination bulls via imported semen. Best prediction was used to calculate actual production (milk, fat, and protein) for 305-d lactations. Adjustment was made for age at calving and milking frequency, and records less than 305 d were projected to 305 d. Herd-year-season (4-mo seasons) and the genetic level of each cow's Holstein maternal grandsire were included in the model for statistical analysis. Pure Holsteins had significantly higher milk (9,757 kg) and protein (305 kg) production than all crossbred groups, but pure Holsteins (346 kg) were not significantly different from Scandinavian Red/Holstein (340 kg) crossbreds for fat production. Fat plus protein production was used to gauge the overall productivity of pure Holsteins vs. crossbreds. The Scandinavian Red/Holstein (637 kg) crossbreds were not significantly different from the pure Holstein (651 kg) for fat plus protein production; however, the Normande/Holstein (596 kg) and the Montbeliarde/Holstein crossbreds (627 kg) had significantly lower fat plus protein production than pure Holsteins.